AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1265890

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265890 MtrunA17_Chr4g0071311 96.599 147 4 1 1 146 1 147 4.77e-99 281
Msa1265890 MtrunA17_Chr8g0341971 40.441 136 58 4 16 144 62 181 2.33e-20 82.4
Msa1265890 MtrunA17_Chr8g0341991 66.667 51 17 0 94 144 123 173 6.27e-18 75.9
Msa1265890 MtrunA17_Chr8g0338031 59.615 52 21 0 93 144 435 486 8.44e-15 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1265890 AT1G55160.2 43.846 130 63 2 17 146 18 137 5.26e-28 101
Msa1265890 AT1G55160.1 43.846 130 63 2 17 146 69 188 1.67e-27 101
Msa1265890 AT2G19530.1 36.022 186 76 5 1 146 1 183 5.26e-25 95.1
Msa1265890 AT1G55160.3 37.931 145 75 3 17 146 69 213 1.95e-22 89.0

Find 58 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTTAATATCTATGTTTA+AGG 0.141011 8_2:+70499013 None:intergenic
CCAAGAAATCTTCGTAATTT+AGG 0.160954 8_2:+70499460 None:intergenic
AAAGTTTCAATCTGATAAAT+AGG 0.234106 8_2:-70499210 Msa1265890:three_prime_UTR
CCTTCACCTGCACCTCCAAC+TGG 0.257972 8_2:-70501170 Msa1265890:CDS
CCTAAATTACGAAGATTTCT+TGG 0.289145 8_2:-70499460 Msa1265890:CDS
CGTTGTTGTTCGTCAGGAAT+TGG 0.294229 8_2:+70499405 None:intergenic
ACAGGACAGTAACGGGTTAA+TGG 0.339171 8_2:-70499138 Msa1265890:three_prime_UTR
ACTGAACTTGTGCTGGGTTC+AGG 0.350977 8_2:+70499486 None:intergenic
TTGCATTATTAATAACTGAT+AGG 0.355557 8_2:-70499235 Msa1265890:three_prime_UTR
CTGACCTCCAAGTGCAAGAT+TGG 0.362880 8_2:+70501100 None:intergenic
CTTTCCAATCTTGCACTTGG+AGG 0.378838 8_2:-70501104 Msa1265890:intron
AACGAATTTGAAGCGTTTCA+TGG 0.395000 8_2:+70501142 None:intergenic
TAGGTCCACTGAACTTGTGC+TGG 0.400803 8_2:+70499479 None:intergenic
TTGCTTTCCAATCTTGCACT+TGG 0.407455 8_2:-70501107 Msa1265890:CDS
ACTTCACGTTGTTGTTCGTC+AGG 0.414100 8_2:+70499399 None:intergenic
CCATACCAATCGTGCATTGT+TGG 0.426333 8_2:-70499046 Msa1265890:three_prime_UTR
ATGGTTACAAGAATGTAGTT+AGG 0.427854 8_2:+70499065 None:intergenic
TCCCTAAAGGCTAAAACTGT+TGG 0.435819 8_2:+70499105 None:intergenic
TTGCAGAACAGGACAGTAAC+GGG 0.439281 8_2:-70499145 Msa1265890:three_prime_UTR
TGAAACGCTTCAAATTCGTT+TGG 0.442328 8_2:-70501139 Msa1265890:CDS
ATATCTGTTGGTTGTATGCC+TGG 0.449142 8_2:-70498877 Msa1265890:three_prime_UTR
GACCTACCAAGCGGGCATAA+TGG 0.466729 8_2:+70498946 None:intergenic
TTTGATGCTTGCTTCCCTAA+AGG 0.470226 8_2:+70499092 None:intergenic
GGTTCCTGCCACAATGCCTA+TGG 0.483180 8_2:-70499439 Msa1265890:CDS
AGGTCCACTGAACTTGTGCT+GGG 0.487155 8_2:+70499480 None:intergenic
GTCAAGAGGAAGTTAAAACT+TGG 0.487763 8_2:+70499260 None:intergenic
ACGAATTTGAAGCGTTTCAT+GGG 0.492853 8_2:+70501143 None:intergenic
GGTAACCCAGTTGGAGGTGC+AGG 0.493808 8_2:+70501164 None:intergenic
CACAATGCCTATGGAAGTGC+GGG 0.493963 8_2:-70499430 Msa1265890:CDS
TGCAAAACGGAGAGATTCAA+TGG 0.496763 8_2:-70499571 Msa1265890:CDS
TCTTCATGTGATATATCTGT+TGG 0.497217 8_2:-70498889 Msa1265890:three_prime_UTR
CCAACAATGCACGATTGGTA+TGG 0.503864 8_2:+70499046 None:intergenic
AGGAAGTTAAAACTTGGGTA+TGG 0.515988 8_2:+70499266 None:intergenic
TTCTGTTTGCAAGTGCAAAA+CGG 0.520014 8_2:-70499584 Msa1265890:CDS
ATTGGAGCCCGCACTTCCAT+AGG 0.522709 8_2:+70499423 None:intergenic
ATAACGATGATGATGATCAC+TGG 0.533190 8_2:+70501218 None:intergenic
TTCATGGGTAACCCAGTTGG+AGG 0.533424 8_2:+70501158 None:intergenic
CGTTTCATGGGTAACCCAGT+TGG 0.537789 8_2:+70501155 None:intergenic
TGAGAACCATTATGCCCGCT+TGG 0.543296 8_2:-70498952 Msa1265890:three_prime_UTR
GTTATCCAACAATGCACGAT+TGG 0.544948 8_2:+70499041 None:intergenic
GTTGGAGGTGCAGGTGAAGG+AGG 0.550866 8_2:+70501173 None:intergenic
CTTCACCTGCACCTCCAACT+GGG 0.560809 8_2:-70501169 Msa1265890:CDS
CCAGTTGGAGGTGCAGGTGA+AGG 0.567865 8_2:+70501170 None:intergenic
AGTGAAACCAAAGGATGCTA+CGG 0.578798 8_2:-70499346 Msa1265890:CDS
TTTGCAGAACAGGACAGTAA+CGG 0.579303 8_2:-70499146 Msa1265890:three_prime_UTR
ACTTCCATAGGCATTGTGGC+AGG 0.581843 8_2:+70499435 None:intergenic
AACCATTATGCCCGCTTGGT+AGG 0.589286 8_2:-70498948 Msa1265890:three_prime_UTR
TGAACCCAGCACAAGTTCAG+TGG 0.591880 8_2:-70499484 Msa1265890:CDS
CCACAATGCCTATGGAAGTG+CGG 0.595839 8_2:-70499431 Msa1265890:CDS
TCAAGAGGAAGTTAAAACTT+GGG 0.599682 8_2:+70499261 None:intergenic
TTAATAATGCAAGAGTCAAG+AGG 0.603817 8_2:+70499246 None:intergenic
AAAGTAGAGACCTACCAAGC+GGG 0.606497 8_2:+70498938 None:intergenic
TAACGATGATGATGATCACT+GGG 0.607328 8_2:+70501219 None:intergenic
GAACTTGTGCTGGGTTCAGG+AGG 0.607480 8_2:+70499489 None:intergenic
CCGCACTTCCATAGGCATTG+TGG 0.608838 8_2:+70499431 None:intergenic
GAAAAGAAAAGTGAAACCAA+AGG 0.630528 8_2:-70499355 Msa1265890:CDS
TGGGTATGGAATTTGCACGC+AGG 0.712652 8_2:+70499280 None:intergenic
AAAAGTAGAGACCTACCAAG+CGG 0.734554 8_2:+70498937 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATTTAATAAAATATTA+TGG - chr8_2:70500184-70500203 Msa1265890:intron 0.0%
!!! AAATATTAACAAATTTTGAT+TGG - chr8_2:70500483-70500502 Msa1265890:intron 10.0%
!!! AATATTAACAAATTTTGATT+GGG - chr8_2:70500484-70500503 Msa1265890:intron 10.0%
!! TATTTCTTATAATTGATTGA+TGG - chr8_2:70499417-70499436 Msa1265890:CDS 15.0%
!!! AACTTTTTATCAGTTTAAAA+AGG - chr8_2:70500081-70500100 Msa1265890:intron 15.0%
!!! ACTTTTTATCAGTTTAAAAA+GGG - chr8_2:70500082-70500101 Msa1265890:intron 15.0%
!!! ATATTATTTGAAATCCTGAA+TGG + chr8_2:70499343-70499362 None:intergenic 20.0%
!!! GTTTTTTTTTTTCCTTTTAC+CGG + chr8_2:70499448-70499467 None:intergenic 20.0%
!! TCATATTTACTAAAGACAAT+CGG + chr8_2:70499475-70499494 None:intergenic 20.0%
!!! AAGGAAATTTTTGAATTCAA+AGG - chr8_2:70499560-70499579 Msa1265890:CDS 20.0%
!!! CAATGATTTTATTGCAAAAT+TGG - chr8_2:70499671-70499690 Msa1265890:intron 20.0%
!!! TGTACGTTTTGTATTTTAAA+AGG - chr8_2:70499809-70499828 Msa1265890:intron 20.0%
!!! GTATTTTAAAAGGAAACTTT+AGG - chr8_2:70499819-70499838 Msa1265890:intron 20.0%
!! TATATAATATCTCACTAAGT+AGG + chr8_2:70500234-70500253 None:intergenic 20.0%
!! ACAAAACATTTATGCATAAA+AGG - chr8_2:70500256-70500275 Msa1265890:intron 20.0%
!!! CTTTTTGATGTTAAGAAAAA+AGG - chr8_2:70500303-70500322 Msa1265890:intron 20.0%
!! GTTAATATTTGAAAAGAGAT+AGG + chr8_2:70500473-70500492 None:intergenic 20.0%
!! AATTAAAAACATAGAATGAG+AGG - chr8_2:70500537-70500556 Msa1265890:intron 20.0%
!! TTGCATTATTAATAACTGAT+AGG - chr8_2:70500968-70500987 Msa1265890:intron 20.0%
!! AAAGTTTCAATCTGATAAAT+AGG - chr8_2:70500993-70501012 Msa1265890:intron 20.0%
!! TAGCTTAATATCTATGTTTA+AGG + chr8_2:70501193-70501212 None:intergenic 20.0%
! TCCTTGAAACACAATAAAAA+TGG + chr8_2:70498937-70498956 None:intergenic 25.0%
!! TTTTCAATCACTGTAATTGT+TGG - chr8_2:70499188-70499207 Msa1265890:three_prime_UTR 25.0%
! CAATATATCAAACAGATGAT+AGG + chr8_2:70499247-70499266 None:intergenic 25.0%
! AAAGCTATAAAAGATGTGTT+AGG + chr8_2:70499270-70499289 None:intergenic 25.0%
! AGTTGGACAAAATTTCAATT+AGG + chr8_2:70499789-70499808 None:intergenic 25.0%
! AAAATACAAAACGTACAAGT+TGG + chr8_2:70499806-70499825 None:intergenic 25.0%
! ACAAAAAAACATCTTTCAAC+TGG + chr8_2:70499867-70499886 None:intergenic 25.0%
! AATATAACCGTAAGTACTAT+CGG + chr8_2:70500132-70500151 None:intergenic 25.0%
!! CCTTTTTATAGCTATTATAC+AGG + chr8_2:70500515-70500534 None:intergenic 25.0%
! CCTGTATAATAGCTATAAAA+AGG - chr8_2:70500512-70500531 Msa1265890:intron 25.0%
! GAATTATCAAATACGTATCA+TGG + chr8_2:70500592-70500611 None:intergenic 25.0%
!!! GCCATTTTTATTGTGTTTCA+AGG - chr8_2:70498933-70498952 Msa1265890:three_prime_UTR 30.0%
AAGTTGACGAGAAATAAGAA+TGG + chr8_2:70499153-70499172 None:intergenic 30.0%
TCTCGTCAACTTATGATTAA+TGG - chr8_2:70499161-70499180 Msa1265890:three_prime_UTR 30.0%
CTCGTCAACTTATGATTAAT+GGG - chr8_2:70499162-70499181 Msa1265890:three_prime_UTR 30.0%
!! TTGTCTTTGTTCGAGTTTTT+GGG - chr8_2:70499607-70499626 Msa1265890:intron 30.0%
ACATTCATTTCGTGAGAAAA+GGG + chr8_2:70499711-70499730 None:intergenic 30.0%
AACATTCATTTCGTGAGAAA+AGG + chr8_2:70499712-70499731 None:intergenic 30.0%
! ATCGGAGAATTTGTACTTTT+TGG - chr8_2:70499758-70499777 Msa1265890:intron 30.0%
CAACAATGTAGTTTAGTGAT+TGG - chr8_2:70499916-70499935 Msa1265890:intron 30.0%
! AACCGTATCAACTCTTTTAA+GGG + chr8_2:70500048-70500067 None:intergenic 30.0%
! AAACCGTATCAACTCTTTTA+AGG + chr8_2:70500049-70500068 None:intergenic 30.0%
AATATCTCACTAAGTAGGAT+TGG + chr8_2:70500229-70500248 None:intergenic 30.0%
TAACATCAAAAAGCCTAGAA+GGG + chr8_2:70500296-70500315 None:intergenic 30.0%
TTAACATCAAAAAGCCTAGA+AGG + chr8_2:70500297-70500316 None:intergenic 30.0%
!! GTTAAGAAAAAAGGTGTCAA+TGG - chr8_2:70500312-70500331 Msa1265890:intron 30.0%
TACAAAAGATTTACCAAGAG+AGG + chr8_2:70500414-70500433 None:intergenic 30.0%
TTATAGCTATTATACAGGCT+TGG + chr8_2:70500510-70500529 None:intergenic 30.0%
CCAAGAAATCTTCGTAATTT+AGG + chr8_2:70500746-70500765 None:intergenic 30.0%
CCTAAATTACGAAGATTTCT+TGG - chr8_2:70500743-70500762 Msa1265890:intron 30.0%
GAAAAGAAAAGTGAAACCAA+AGG - chr8_2:70500848-70500867 Msa1265890:intron 30.0%
TCAAGAGGAAGTTAAAACTT+GGG + chr8_2:70500945-70500964 None:intergenic 30.0%
TTAATAATGCAAGAGTCAAG+AGG + chr8_2:70500960-70500979 None:intergenic 30.0%
!!! GTTCATTTTTTTTGCAGAAC+AGG - chr8_2:70501047-70501066 Msa1265890:intron 30.0%
ATGGTTACAAGAATGTAGTT+AGG + chr8_2:70501141-70501160 None:intergenic 30.0%
TCTTCATGTGATATATCTGT+TGG - chr8_2:70501314-70501333 Msa1265890:five_prime_UTR 30.0%
AAGCTCGAAAAAACAGAGAA+AGG - chr8_2:70498882-70498901 Msa1265890:three_prime_UTR 35.0%
AGCTCGAAAAAACAGAGAAA+GGG - chr8_2:70498883-70498902 Msa1265890:three_prime_UTR 35.0%
! TAACGATGATGATGATCACT+GGG + chr8_2:70498987-70499006 None:intergenic 35.0%
! ATAACGATGATGATGATCAC+TGG + chr8_2:70498988-70499007 None:intergenic 35.0%
! ACGAATTTGAAGCGTTTCAT+GGG + chr8_2:70499063-70499082 None:intergenic 35.0%
! AACGAATTTGAAGCGTTTCA+TGG + chr8_2:70499064-70499083 None:intergenic 35.0%
TGAAACGCTTCAAATTCGTT+TGG - chr8_2:70499064-70499083 Msa1265890:three_prime_UTR 35.0%
ACGAGAAATAAGAATGGAGA+TGG + chr8_2:70499147-70499166 None:intergenic 35.0%
AACTTTGCAAGAAACCATTC+AGG - chr8_2:70499326-70499345 Msa1265890:CDS 35.0%
! TAATTGATTGATGGTTGTGC+CGG - chr8_2:70499426-70499445 Msa1265890:CDS 35.0%
! AGTGTTTCTGTCACATCATT+TGG - chr8_2:70499528-70499547 Msa1265890:CDS 35.0%
CATCATTTGGCATTCAAGAA+AGG - chr8_2:70499541-70499560 Msa1265890:CDS 35.0%
AAGGTGATGCAGTTGAATTA+TGG - chr8_2:70499579-70499598 Msa1265890:CDS 35.0%
!! CTTGTCTTTGTTCGAGTTTT+TGG - chr8_2:70499606-70499625 Msa1265890:intron 35.0%
!! TGTCTTTGTTCGAGTTTTTG+GGG - chr8_2:70499608-70499627 Msa1265890:intron 35.0%
! TCTTTCAACTGGCTTAACAA+CGG + chr8_2:70499856-70499875 None:intergenic 35.0%
GGGACAAAATCATCAGTAAT+TGG + chr8_2:70500028-70500047 None:intergenic 35.0%
!! GTCCCTTAAAAGAGTTGATA+CGG - chr8_2:70500043-70500062 Msa1265890:intron 35.0%
AGTTTGACCGATAGTACTTA+CGG - chr8_2:70500122-70500141 Msa1265890:intron 35.0%
ACCTACAATATACTGATGTG+TGG - chr8_2:70500380-70500399 Msa1265890:intron 35.0%
CCTACAATATACTGATGTGT+GGG - chr8_2:70500381-70500400 Msa1265890:intron 35.0%
AATATACTGATGTGTGGGAA+TGG - chr8_2:70500386-70500405 Msa1265890:intron 35.0%
TTCTGTTTGCAAGTGCAAAA+CGG - chr8_2:70500619-70500638 Msa1265890:intron 35.0%
!! AACAACGTGAAGTTTTCAAG+TGG - chr8_2:70500814-70500833 Msa1265890:intron 35.0%
!!! TGAAGTTTTCAAGTGGATTC+TGG - chr8_2:70500821-70500840 Msa1265890:intron 35.0%
AGGAAGTTAAAACTTGGGTA+TGG + chr8_2:70500940-70500959 None:intergenic 35.0%
GTCAAGAGGAAGTTAAAACT+TGG + chr8_2:70500946-70500965 None:intergenic 35.0%
!! TTCCAACAGTTTTAGCCTTT+AGG - chr8_2:70501096-70501115 Msa1265890:intron 35.0%
!! TCCAACAGTTTTAGCCTTTA+GGG - chr8_2:70501097-70501116 Msa1265890:intron 35.0%
CAATAAAAATGGCGTGAGTG+TGG + chr8_2:70498926-70498945 None:intergenic 40.0%
TTGCTTTCCAATCTTGCACT+TGG - chr8_2:70499096-70499115 Msa1265890:three_prime_UTR 40.0%
GACAAGGAAACAACACATTG+TGG + chr8_2:70499214-70499233 None:intergenic 40.0%
CGTGTCTTTATTAAGCTAGC+AGG - chr8_2:70499296-70499315 Msa1265890:CDS 40.0%
ATGTAACTGCATGCTGTGAT+CGG - chr8_2:70499740-70499759 Msa1265890:intron 40.0%
! TTTCAACTGGCTTAACAACG+GGG + chr8_2:70499854-70499873 None:intergenic 40.0%
! CTTTCAACTGGCTTAACAAC+GGG + chr8_2:70499855-70499874 None:intergenic 40.0%
TAACACCATACTTTGCAGTC+TGG - chr8_2:70499954-70499973 Msa1265890:intron 40.0%
!! CTAAGTAGGATTGGCTACAT+GGG + chr8_2:70500220-70500239 None:intergenic 40.0%
!! ACTAAGTAGGATTGGCTACA+TGG + chr8_2:70500221-70500240 None:intergenic 40.0%
CCCACACATCAGTATATTGT+AGG + chr8_2:70500384-70500403 None:intergenic 40.0%
TGCAAAACGGAGAGATTCAA+TGG - chr8_2:70500632-70500651 Msa1265890:intron 40.0%
AGTGAAACCAAAGGATGCTA+CGG - chr8_2:70500857-70500876 Msa1265890:intron 40.0%
! TTTTTCTCCGTAGCATCCTT+TGG + chr8_2:70500867-70500886 None:intergenic 40.0%
TTTGCAGAACAGGACAGTAA+CGG - chr8_2:70501057-70501076 Msa1265890:intron 40.0%
TCCCTAAAGGCTAAAACTGT+TGG + chr8_2:70501101-70501120 None:intergenic 40.0%
TTTGATGCTTGCTTCCCTAA+AGG + chr8_2:70501114-70501133 None:intergenic 40.0%
GTTATCCAACAATGCACGAT+TGG + chr8_2:70501165-70501184 None:intergenic 40.0%
AAAAGTAGAGACCTACCAAG+CGG + chr8_2:70501269-70501288 None:intergenic 40.0%
CTTTCCAATCTTGCACTTGG+AGG - chr8_2:70499099-70499118 Msa1265890:three_prime_UTR 45.0%
GATAGGCAAAATGCGTGACA+AGG + chr8_2:70499230-70499249 None:intergenic 45.0%
! TTGATGGTTGTGCCGGTAAA+AGG - chr8_2:70499433-70499452 Msa1265890:CDS 45.0%
!! TGTTCGAGTTTTTGGGGACA+TGG - chr8_2:70499614-70499633 Msa1265890:intron 45.0%
!! GTTCGAGTTTTTGGGGACAT+GGG - chr8_2:70499615-70499634 Msa1265890:intron 45.0%
! TTCAACTGGCTTAACAACGG+GGG + chr8_2:70499853-70499872 None:intergenic 45.0%
ACCATACTTTGCAGTCTGGT+GGG - chr8_2:70499958-70499977 Msa1265890:intron 45.0%
GTAGAGACAGTGACTTCACA+TGG + chr8_2:70500006-70500025 None:intergenic 45.0%
CTAGAAGGGTTGAGAAGTTG+AGG + chr8_2:70500282-70500301 None:intergenic 45.0%
CTCAACTTCTCAACCCTTCT+AGG - chr8_2:70500280-70500299 Msa1265890:intron 45.0%
! CGTTGTTGTTCGTCAGGAAT+TGG + chr8_2:70500801-70500820 None:intergenic 45.0%
! ACTTCACGTTGTTGTTCGTC+AGG + chr8_2:70500807-70500826 None:intergenic 45.0%
TTGCAGAACAGGACAGTAAC+GGG - chr8_2:70501058-70501077 Msa1265890:intron 45.0%
!! ACAGGACAGTAACGGGTTAA+TGG - chr8_2:70501065-70501084 Msa1265890:intron 45.0%
CCAACAATGCACGATTGGTA+TGG + chr8_2:70501160-70501179 None:intergenic 45.0%
CCATACCAATCGTGCATTGT+TGG - chr8_2:70501157-70501176 Msa1265890:CDS 45.0%
AAAGTAGAGACCTACCAAGC+GGG + chr8_2:70501268-70501287 None:intergenic 45.0%
TTCATGGGTAACCCAGTTGG+AGG + chr8_2:70499048-70499067 None:intergenic 50.0%
CGTTTCATGGGTAACCCAGT+TGG + chr8_2:70499051-70499070 None:intergenic 50.0%
CTGACCTCCAAGTGCAAGAT+TGG + chr8_2:70499106-70499125 None:intergenic 50.0%
!! GAGTTTTTGGGGACATGGGA+AGG - chr8_2:70499619-70499638 Msa1265890:intron 50.0%
!! GACATGGGAAGGAGCATCTA+CGG - chr8_2:70499630-70499649 Msa1265890:intron 50.0%
!! ACATGGGAAGGAGCATCTAC+GGG - chr8_2:70499631-70499650 Msa1265890:intron 50.0%
CACCATACTTTGCAGTCTGG+TGG - chr8_2:70499957-70499976 Msa1265890:intron 50.0%
CCATACTTTGCAGTCTGGTG+GGG - chr8_2:70499959-70499978 Msa1265890:intron 50.0%
TGTGGGAATGGTTCCTCTCT+TGG - chr8_2:70500398-70500417 Msa1265890:intron 50.0%
ACTGAACTTGTGCTGGGTTC+AGG + chr8_2:70500720-70500739 None:intergenic 50.0%
TGAACCCAGCACAAGTTCAG+TGG - chr8_2:70500719-70500738 Msa1265890:intron 50.0%
AGGTCCACTGAACTTGTGCT+GGG + chr8_2:70500726-70500745 None:intergenic 50.0%
TAGGTCCACTGAACTTGTGC+TGG + chr8_2:70500727-70500746 None:intergenic 50.0%
! ACTTCCATAGGCATTGTGGC+AGG + chr8_2:70500771-70500790 None:intergenic 50.0%
CCACAATGCCTATGGAAGTG+CGG - chr8_2:70500772-70500791 Msa1265890:intron 50.0%
! CACAATGCCTATGGAAGTGC+GGG - chr8_2:70500773-70500792 Msa1265890:intron 50.0%
TGGGTATGGAATTTGCACGC+AGG + chr8_2:70500926-70500945 None:intergenic 50.0%
TGAGAACCATTATGCCCGCT+TGG - chr8_2:70501251-70501270 Msa1265890:five_prime_UTR 50.0%
AACCATTATGCCCGCTTGGT+AGG - chr8_2:70501255-70501274 Msa1265890:five_prime_UTR 50.0%
CTTCACCTGCACCTCCAACT+GGG - chr8_2:70499034-70499053 Msa1265890:three_prime_UTR 55.0%
CCCCACCAGACTGCAAAGTA+TGG + chr8_2:70499962-70499981 None:intergenic 55.0%
GAACTTGTGCTGGGTTCAGG+AGG + chr8_2:70500717-70500736 None:intergenic 55.0%
GGTTCCTGCCACAATGCCTA+TGG - chr8_2:70500764-70500783 Msa1265890:intron 55.0%
! CCGCACTTCCATAGGCATTG+TGG + chr8_2:70500775-70500794 None:intergenic 55.0%
ATTGGAGCCCGCACTTCCAT+AGG + chr8_2:70500783-70500802 None:intergenic 55.0%
GACCTACCAAGCGGGCATAA+TGG + chr8_2:70501260-70501279 None:intergenic 55.0%
! GTTGGAGGTGCAGGTGAAGG+AGG + chr8_2:70499033-70499052 None:intergenic 60.0%
! CCAGTTGGAGGTGCAGGTGA+AGG + chr8_2:70499036-70499055 None:intergenic 60.0%
CCTTCACCTGCACCTCCAAC+TGG - chr8_2:70499033-70499052 Msa1265890:three_prime_UTR 60.0%
! GGTAACCCAGTTGGAGGTGC+AGG + chr8_2:70499042-70499061 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr8_2 gene 70498877 70501348 70498877 ID=Msa1265890;Name=Msa1265890
chr8_2 mRNA 70498877 70501348 70498877 ID=Msa1265890-mRNA-1;Parent=Msa1265890;Name=Msa1265890-mRNA-1;_AED=0.00;_eAED=0.00;_QI=141|1|1|1|1|1|2|395|146
chr8_2 exon 70498877 70499609 70498877 ID=Msa1265890-mRNA-1:exon:23426;Parent=Msa1265890-mRNA-1
chr8_2 exon 70501105 70501348 70501105 ID=Msa1265890-mRNA-1:exon:23425;Parent=Msa1265890-mRNA-1
chr8_2 five_prime_UTR 70501208 70501348 70501208 ID=Msa1265890-mRNA-1:five_prime_utr;Parent=Msa1265890-mRNA-1
chr8_2 CDS 70501105 70501207 70501105 ID=Msa1265890-mRNA-1:cds;Parent=Msa1265890-mRNA-1
chr8_2 CDS 70499272 70499609 70499272 ID=Msa1265890-mRNA-1:cds;Parent=Msa1265890-mRNA-1
chr8_2 three_prime_UTR 70498877 70499271 70498877 ID=Msa1265890-mRNA-1:three_prime_utr;Parent=Msa1265890-mRNA-1
Gene Sequence

>Msa1265890

ATGAATGAGACTCCTCCTTCACCTGCACCTCCAACTGGGTTACCCATGAAACGCTTCAAATTCGTTTGGCGCTTTCTCTTGCTTTCCAATCTTGCACTTGGAGCTTTTCTGTTTGCAAGTGCAAAACGGAGAGATTCAATGGAGATTCAGAAAAAAAGAACAGCACACAGATTGCATAAGAGCAAAGCTGCTGTCGAAGTTCCTCCTGAACCCAGCACAAGTTCAGTGGACCTAAATTACGAAGATTTCTTGGTTCCTGCCACAATGCCTATGGAAGTGCGGGCTCCAATTCCTGACGAACAACAACGTGAAGTTTTCAAGTGGATTCTGGAAGAGAAAAGAAAAGTGAAACCAAAGGATGCTACGGAGAAAAAACAAATTGACGAAGACAAGTACATTCTTAAGCAGTTCCTGCGTGCAAATTCCATACCCAAGTTTTAA
Protein sequence

>Msa1265890

MNETPPSPAPPTGLPMKRFKFVWRFLLLSNLALGAFLFASAKRRDSMEIQKKRTAHRLHKSKAAVEVPPEPSTSSVDLNYEDFLVPATMPMEVRAPIPDEQQREVFKWILEEKRKVKPKDATEKKQIDEDKYILKQFLRANSIPKF*