AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1327980


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGGAGCCGGAAATAAAATT+TGG 0.310961 8_4:-45685369 Msa1327980:CDS
GTCGTATACAGCGGCGGAGC+CGG 0.460313 8_4:-45685382 Msa1327980:CDS
CGTACTGGAGTTAAGTGGTA+TGG 0.485176 8_4:-45685520 Msa1327980:CDS
TAGAATATGATAAAACGTAC+TGG 0.488816 8_4:-45685535 Msa1327980:CDS
AATGAGACGGTGATATGGGA+TGG 0.537767 8_4:+45685481 None:intergenic
AGCCGGAAATAAAATTTGGA+TGG 0.541216 8_4:-45685365 Msa1327980:CDS
ATGACATTGTTACAATGTGA+TGG 0.550818 8_4:+45685436 None:intergenic
GCCGGAAATAAAATTTGGAT+GGG 0.580839 8_4:-45685364 Msa1327980:CDS
GACGGTGATATGGGATGGAG+TGG 0.602020 8_4:+45685486 None:intergenic
TAAAACGTACTGGAGTTAAG+TGG 0.625133 8_4:-45685525 Msa1327980:CDS
TGACATTGTTACAATGTGAT+GGG 0.627569 8_4:+45685437 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTAGAAAATATAAAATA+TGG + chr8_4:45685426-45685445 None:intergenic 10.0%
!! AAAGTAGAAAATATAAAATA+TGG + chr8_4:45685426-45685445 None:intergenic 10.0%
!!! ATTAAGAATTTTTTAACTTC+TGG - chr8_4:45685394-45685413 Msa1327980:CDS 15.0%
!!! TTAAGAATTTTTTAACTTCT+GGG - chr8_4:45685395-45685414 Msa1327980:CDS 15.0%
!!! TCTACAAATGTATTTTATTT+AGG - chr8_4:45685445-45685464 Msa1327980:CDS 15.0%
!! AATCATATTATTTATGTCTA+TGG - chr8_4:45686301-45686320 Msa1327980:intron 15.0%
!!! ATTAAGAATTTTTTAACTTC+TGG - chr8_4:45685394-45685413 Msa1327980:CDS 15.0%
!!! TTAAGAATTTTTTAACTTCT+GGG - chr8_4:45685395-45685414 Msa1327980:CDS 15.0%
!!! TCTACAAATGTATTTTATTT+AGG - chr8_4:45685445-45685464 Msa1327980:CDS 15.0%
!! AATCATATTATTTATGTCTA+TGG - chr8_4:45686301-45686320 Msa1327980:intron 15.0%
!! AAATGAATGAAAATATAGAC+TGG - chr8_4:45685530-45685549 Msa1327980:CDS 20.0%
!!! CTATTTTCGTTTTTGTTAAT+GGG - chr8_4:45686124-45686143 Msa1327980:intron 20.0%
!!! TATTTTCGTTTTTGTTAATG+GGG - chr8_4:45686125-45686144 Msa1327980:intron 20.0%
!! ATAAACATAACATAAGCAAA+TGG + chr8_4:45686522-45686541 None:intergenic 20.0%
!! AGAAAATATCAGTTTCATTA+TGG + chr8_4:45686649-45686668 None:intergenic 20.0%
!! ACCAATTATAAAAAATGAGA+CGG + chr8_4:45686750-45686769 None:intergenic 20.0%
!! AAATGAATGAAAATATAGAC+TGG - chr8_4:45685530-45685549 Msa1327980:CDS 20.0%
!!! CTATTTTCGTTTTTGTTAAT+GGG - chr8_4:45686124-45686143 Msa1327980:intron 20.0%
!!! TATTTTCGTTTTTGTTAATG+GGG - chr8_4:45686125-45686144 Msa1327980:intron 20.0%
!! ATAAACATAACATAAGCAAA+TGG + chr8_4:45686522-45686541 None:intergenic 20.0%
!! AGAAAATATCAGTTTCATTA+TGG + chr8_4:45686649-45686668 None:intergenic 20.0%
!! ACCAATTATAAAAAATGAGA+CGG + chr8_4:45686750-45686769 None:intergenic 20.0%
! TAGGAAGTTATATTATGATG+TGG - chr8_4:45685464-45685483 Msa1327980:CDS 25.0%
!!! TCTTATTTCTGCTAACTAAT+AGG - chr8_4:45685710-45685729 Msa1327980:intron 25.0%
!!! CTTATTTCTGCTAACTAATA+GGG - chr8_4:45685711-45685730 Msa1327980:intron 25.0%
!!! TTATTTCTGCTAACTAATAG+GGG - chr8_4:45685712-45685731 Msa1327980:intron 25.0%
!! TACTCATAATTTGCAAGAAA+TGG + chr8_4:45685966-45685985 None:intergenic 25.0%
! AGCAAATATCTATAGAAGTA+TGG + chr8_4:45686043-45686062 None:intergenic 25.0%
!!! CTATAGATATTTGCTGATTT+AGG - chr8_4:45686048-45686067 Msa1327980:intron 25.0%
!!! CTGTTTTAAAGTTTTGTTCT+TGG - chr8_4:45686091-45686110 Msa1327980:intron 25.0%
! CCATTAACAAAAACGAAAAT+AGG + chr8_4:45686126-45686145 None:intergenic 25.0%
!!! CCTATTTTCGTTTTTGTTAA+TGG - chr8_4:45686123-45686142 Msa1327980:intron 25.0%
!!! TCAACTTACTTCTTTAGTTT+TGG - chr8_4:45686191-45686210 Msa1327980:intron 25.0%
! AGTTGTTCTATAATGCTAAA+TGG - chr8_4:45686238-45686257 Msa1327980:intron 25.0%
! TAATGTTAAGTTAATGTTGC+AGG - chr8_4:45686344-45686363 Msa1327980:intron 25.0%
!!! ACTTTGTTAGCTTATTGTTT+TGG - chr8_4:45686447-45686466 Msa1327980:intron 25.0%
!! CTGTATTTTGCATTTTATCA+AGG - chr8_4:45686480-45686499 Msa1327980:intron 25.0%
! TAGAATATGATAAAACGTAC+TGG - chr8_4:45686680-45686699 Msa1327980:intron 25.0%
!!! ACCGTCTCATTTTTTATAAT+TGG - chr8_4:45686746-45686765 Msa1327980:intron 25.0%
! TAGGAAGTTATATTATGATG+TGG - chr8_4:45685464-45685483 Msa1327980:CDS 25.0%
!!! TCTTATTTCTGCTAACTAAT+AGG - chr8_4:45685710-45685729 Msa1327980:intron 25.0%
!!! CTTATTTCTGCTAACTAATA+GGG - chr8_4:45685711-45685730 Msa1327980:intron 25.0%
!!! TTATTTCTGCTAACTAATAG+GGG - chr8_4:45685712-45685731 Msa1327980:intron 25.0%
!! TACTCATAATTTGCAAGAAA+TGG + chr8_4:45685966-45685985 None:intergenic 25.0%
! AGCAAATATCTATAGAAGTA+TGG + chr8_4:45686043-45686062 None:intergenic 25.0%
!!! CTATAGATATTTGCTGATTT+AGG - chr8_4:45686048-45686067 Msa1327980:intron 25.0%
!!! CTGTTTTAAAGTTTTGTTCT+TGG - chr8_4:45686091-45686110 Msa1327980:intron 25.0%
! CCATTAACAAAAACGAAAAT+AGG + chr8_4:45686126-45686145 None:intergenic 25.0%
!!! CCTATTTTCGTTTTTGTTAA+TGG - chr8_4:45686123-45686142 Msa1327980:intron 25.0%
!!! TCAACTTACTTCTTTAGTTT+TGG - chr8_4:45686191-45686210 Msa1327980:intron 25.0%
! AGTTGTTCTATAATGCTAAA+TGG - chr8_4:45686238-45686257 Msa1327980:intron 25.0%
! TAATGTTAAGTTAATGTTGC+AGG - chr8_4:45686344-45686363 Msa1327980:intron 25.0%
!!! ACTTTGTTAGCTTATTGTTT+TGG - chr8_4:45686447-45686466 Msa1327980:intron 25.0%
!! CTGTATTTTGCATTTTATCA+AGG - chr8_4:45686480-45686499 Msa1327980:intron 25.0%
! TAGAATATGATAAAACGTAC+TGG - chr8_4:45686680-45686699 Msa1327980:intron 25.0%
!!! ACCGTCTCATTTTTTATAAT+TGG - chr8_4:45686746-45686765 Msa1327980:intron 25.0%
TTTATTCCTTTCCCATAAGA+TGG + chr8_4:45685354-45685373 None:intergenic 30.0%
! TATCAGTAAGATTTGGTCAA+TGG + chr8_4:45685493-45685512 None:intergenic 30.0%
ATACGAGTTGTTTATTGTAG+AGG - chr8_4:45685578-45685597 Msa1327980:intron 30.0%
ACTTAGAAATTGTATCAGTC+TGG + chr8_4:45685614-45685633 None:intergenic 30.0%
! TATTTCTGCTAACTAATAGG+GGG - chr8_4:45685713-45685732 Msa1327980:intron 30.0%
! CATATCGCTTACAATTTGTT+TGG - chr8_4:45685742-45685761 Msa1327980:intron 30.0%
!! GAGAATGTCATTGATTTAGT+TGG + chr8_4:45686173-45686192 None:intergenic 30.0%
! CTTGTTGGTTACAAATTTGA+CGG - chr8_4:45686416-45686435 Msa1327980:intron 30.0%
AAAAAATGAGACGGTGATAT+GGG + chr8_4:45686741-45686760 None:intergenic 30.0%
TAAAAAATGAGACGGTGATA+TGG + chr8_4:45686742-45686761 None:intergenic 30.0%
TGACATTGTTACAATGTGAT+GGG + chr8_4:45686781-45686800 None:intergenic 30.0%
ATGACATTGTTACAATGTGA+TGG + chr8_4:45686782-45686801 None:intergenic 30.0%
TTTATTCCTTTCCCATAAGA+TGG + chr8_4:45685354-45685373 None:intergenic 30.0%
! TATCAGTAAGATTTGGTCAA+TGG + chr8_4:45685493-45685512 None:intergenic 30.0%
ATACGAGTTGTTTATTGTAG+AGG - chr8_4:45685578-45685597 Msa1327980:intron 30.0%
ACTTAGAAATTGTATCAGTC+TGG + chr8_4:45685614-45685633 None:intergenic 30.0%
! TATTTCTGCTAACTAATAGG+GGG - chr8_4:45685713-45685732 Msa1327980:intron 30.0%
! CATATCGCTTACAATTTGTT+TGG - chr8_4:45685742-45685761 Msa1327980:intron 30.0%
!! GAGAATGTCATTGATTTAGT+TGG + chr8_4:45686173-45686192 None:intergenic 30.0%
! CTTGTTGGTTACAAATTTGA+CGG - chr8_4:45686416-45686435 Msa1327980:intron 30.0%
AAAAAATGAGACGGTGATAT+GGG + chr8_4:45686741-45686760 None:intergenic 30.0%
TAAAAAATGAGACGGTGATA+TGG + chr8_4:45686742-45686761 None:intergenic 30.0%
TGACATTGTTACAATGTGAT+GGG + chr8_4:45686781-45686800 None:intergenic 30.0%
ATGACATTGTTACAATGTGA+TGG + chr8_4:45686782-45686801 None:intergenic 30.0%
! ATGGTAGTGTTACCATCTTA+TGG - chr8_4:45685339-45685358 Msa1327980:CDS 35.0%
! TGGTAGTGTTACCATCTTAT+GGG - chr8_4:45685340-45685359 Msa1327980:CDS 35.0%
TGACCAAATCTTACTGATAG+AGG - chr8_4:45685494-45685513 Msa1327980:CDS 35.0%
GACCAAATCTTACTGATAGA+GGG - chr8_4:45685495-45685514 Msa1327980:CDS 35.0%
AACCCTCTATCAGTAAGATT+TGG + chr8_4:45685500-45685519 None:intergenic 35.0%
AAATTGTATCAGTCTGGTGA+AGG + chr8_4:45685608-45685627 None:intergenic 35.0%
TGTTAGTGACGTTATAAGGA+TGG + chr8_4:45685658-45685677 None:intergenic 35.0%
CTTGTGTTAGTGACGTTATA+AGG + chr8_4:45685662-45685681 None:intergenic 35.0%
AGAACGAGTTTCATCATCTA+TGG - chr8_4:45685684-45685703 Msa1327980:intron 35.0%
!! TGGCTCAGATGATTTTGAAA+TGG + chr8_4:45686023-45686042 None:intergenic 35.0%
AGGATGAATCAGTGTAGATT+TGG - chr8_4:45686364-45686383 Msa1327980:intron 35.0%
GGTTATTCAATCTTCCTTGT+TGG - chr8_4:45686401-45686420 Msa1327980:intron 35.0%
CGTCAAATTTGTAACCAACA+AGG + chr8_4:45686418-45686437 None:intergenic 35.0%
GTTTATCTTGTGTAGAACAG+AGG - chr8_4:45686536-45686555 Msa1327980:intron 35.0%
GCTATAACTCTGTAAAGTTG+TGG - chr8_4:45686609-45686628 Msa1327980:intron 35.0%
TAAAACGTACTGGAGTTAAG+TGG - chr8_4:45686690-45686709 Msa1327980:intron 35.0%
AGCCGGAAATAAAATTTGGA+TGG - chr8_4:45686850-45686869 Msa1327980:intron 35.0%
GCCGGAAATAAAATTTGGAT+GGG - chr8_4:45686851-45686870 Msa1327980:intron 35.0%
!! GCCCATCCAAATTTTATTTC+CGG + chr8_4:45686855-45686874 None:intergenic 35.0%
! ATGGTAGTGTTACCATCTTA+TGG - chr8_4:45685339-45685358 Msa1327980:CDS 35.0%
! TGGTAGTGTTACCATCTTAT+GGG - chr8_4:45685340-45685359 Msa1327980:CDS 35.0%
TGACCAAATCTTACTGATAG+AGG - chr8_4:45685494-45685513 Msa1327980:CDS 35.0%
GACCAAATCTTACTGATAGA+GGG - chr8_4:45685495-45685514 Msa1327980:CDS 35.0%
AACCCTCTATCAGTAAGATT+TGG + chr8_4:45685500-45685519 None:intergenic 35.0%
AAATTGTATCAGTCTGGTGA+AGG + chr8_4:45685608-45685627 None:intergenic 35.0%
TGTTAGTGACGTTATAAGGA+TGG + chr8_4:45685658-45685677 None:intergenic 35.0%
CTTGTGTTAGTGACGTTATA+AGG + chr8_4:45685662-45685681 None:intergenic 35.0%
AGAACGAGTTTCATCATCTA+TGG - chr8_4:45685684-45685703 Msa1327980:intron 35.0%
!! TGGCTCAGATGATTTTGAAA+TGG + chr8_4:45686023-45686042 None:intergenic 35.0%
AGGATGAATCAGTGTAGATT+TGG - chr8_4:45686364-45686383 Msa1327980:intron 35.0%
GGTTATTCAATCTTCCTTGT+TGG - chr8_4:45686401-45686420 Msa1327980:intron 35.0%
CGTCAAATTTGTAACCAACA+AGG + chr8_4:45686418-45686437 None:intergenic 35.0%
GTTTATCTTGTGTAGAACAG+AGG - chr8_4:45686536-45686555 Msa1327980:intron 35.0%
GCTATAACTCTGTAAAGTTG+TGG - chr8_4:45686609-45686628 Msa1327980:intron 35.0%
TAAAACGTACTGGAGTTAAG+TGG - chr8_4:45686690-45686709 Msa1327980:intron 35.0%
AGCCGGAAATAAAATTTGGA+TGG - chr8_4:45686850-45686869 Msa1327980:intron 35.0%
GCCGGAAATAAAATTTGGAT+GGG - chr8_4:45686851-45686870 Msa1327980:intron 35.0%
!! GCCCATCCAAATTTTATTTC+CGG + chr8_4:45686855-45686874 None:intergenic 35.0%
GTGTTACCATCTTATGGGAA+AGG - chr8_4:45685345-45685364 Msa1327980:CDS 40.0%
TGACGTTATAAGGATGGTGA+GGG + chr8_4:45685652-45685671 None:intergenic 40.0%
CAACACTGTCTACATCAAGA+GGG + chr8_4:45685783-45685802 None:intergenic 40.0%
GATTTGGCATTTGCACATTC+TGG - chr8_4:45686380-45686399 Msa1327980:intron 40.0%
GTGTTACCATCTTATGGGAA+AGG - chr8_4:45685345-45685364 Msa1327980:CDS 40.0%
TGACGTTATAAGGATGGTGA+GGG + chr8_4:45685652-45685671 None:intergenic 40.0%
CAACACTGTCTACATCAAGA+GGG + chr8_4:45685783-45685802 None:intergenic 40.0%
GATTTGGCATTTGCACATTC+TGG - chr8_4:45686380-45686399 Msa1327980:intron 40.0%
TTGTATCAGTCTGGTGAAGG+AGG + chr8_4:45685605-45685624 None:intergenic 45.0%
GACGTTATAAGGATGGTGAG+GGG + chr8_4:45685651-45685670 None:intergenic 45.0%
GTGACGTTATAAGGATGGTG+AGG + chr8_4:45685653-45685672 None:intergenic 45.0%
GCAACACTGTCTACATCAAG+AGG + chr8_4:45685784-45685803 None:intergenic 45.0%
CGTACTGGAGTTAAGTGGTA+TGG - chr8_4:45686695-45686714 Msa1327980:intron 45.0%
AATGAGACGGTGATATGGGA+TGG + chr8_4:45686737-45686756 None:intergenic 45.0%
GCGGAGCCGGAAATAAAATT+TGG - chr8_4:45686846-45686865 Msa1327980:intron 45.0%
TTGTATCAGTCTGGTGAAGG+AGG + chr8_4:45685605-45685624 None:intergenic 45.0%
GACGTTATAAGGATGGTGAG+GGG + chr8_4:45685651-45685670 None:intergenic 45.0%
GTGACGTTATAAGGATGGTG+AGG + chr8_4:45685653-45685672 None:intergenic 45.0%
GCAACACTGTCTACATCAAG+AGG + chr8_4:45685784-45685803 None:intergenic 45.0%
CGTACTGGAGTTAAGTGGTA+TGG - chr8_4:45686695-45686714 Msa1327980:intron 45.0%
AATGAGACGGTGATATGGGA+TGG + chr8_4:45686737-45686756 None:intergenic 45.0%
GCGGAGCCGGAAATAAAATT+TGG - chr8_4:45686846-45686865 Msa1327980:intron 45.0%
GACGGTGATATGGGATGGAG+TGG + chr8_4:45686732-45686751 None:intergenic 55.0%
GCGTGTCTCGTCGTATACAG+CGG - chr8_4:45686824-45686843 Msa1327980:intron 55.0%
TGTCTCGTCGTATACAGCGG+CGG - chr8_4:45686827-45686846 Msa1327980:intron 55.0%
GACGGTGATATGGGATGGAG+TGG + chr8_4:45686732-45686751 None:intergenic 55.0%
GCGTGTCTCGTCGTATACAG+CGG - chr8_4:45686824-45686843 Msa1327980:intron 55.0%
TGTCTCGTCGTATACAGCGG+CGG - chr8_4:45686827-45686846 Msa1327980:intron 55.0%
GGATGGTGAGGGGCAGAGTA+AGG + chr8_4:45685641-45685660 None:intergenic 60.0%
GGATGGTGAGGGGCAGAGTA+AGG + chr8_4:45685641-45685660 None:intergenic 60.0%
GTCGTATACAGCGGCGGAGC+CGG - chr8_4:45686833-45686852 Msa1327980:intron 65.0%
GTCGTATACAGCGGCGGAGC+CGG - chr8_4:45686833-45686852 Msa1327980:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 45685339 45686898 45685339 ID=Msa1327980;Name=Msa1327980
chr8_4 mRNA 45685339 45686898 45685339 ID=Msa1327980-mRNA-1;Parent=Msa1327980;Name=Msa1327980-mRNA-1;_AED=0.94;_eAED=0.94;_QI=0|0.25|0|0.8|1|1|5|0|80
chr8_4 exon 45686893 45686898 45686893 ID=Msa1327980-mRNA-1:exon:12863;Parent=Msa1327980-mRNA-1
chr8_4 exon 45685756 45685764 45685756 ID=Msa1327980-mRNA-1:exon:12862;Parent=Msa1327980-mRNA-1
chr8_4 exon 45685730 45685735 45685730 ID=Msa1327980-mRNA-1:exon:12861;Parent=Msa1327980-mRNA-1
chr8_4 exon 45685676 45685681 45685676 ID=Msa1327980-mRNA-1:exon:12860;Parent=Msa1327980-mRNA-1
chr8_4 exon 45685339 45685554 45685339 ID=Msa1327980-mRNA-1:exon:12859;Parent=Msa1327980-mRNA-1
chr8_4 CDS 45686893 45686898 45686893 ID=Msa1327980-mRNA-1:cds;Parent=Msa1327980-mRNA-1
chr8_4 CDS 45685756 45685764 45685756 ID=Msa1327980-mRNA-1:cds;Parent=Msa1327980-mRNA-1
chr8_4 CDS 45685730 45685735 45685730 ID=Msa1327980-mRNA-1:cds;Parent=Msa1327980-mRNA-1
chr8_4 CDS 45685676 45685681 45685676 ID=Msa1327980-mRNA-1:cds;Parent=Msa1327980-mRNA-1
chr8_4 CDS 45685339 45685554 45685339 ID=Msa1327980-mRNA-1:cds;Parent=Msa1327980-mRNA-1
Gene Sequence

>Msa1327980

ATGGTATGTACTTCTGCTCAAAGGACAAATATGATAAAACGTACTGGAGTTAAGTGGTATGGAGTAACAAAATCCACTCCATCCCATATCACCGTCTCATTTTTTATAATTGGTCATTTTTTCCCATCACATTGTAACAATGTCATTCTCATGTGTTTCTTCTCTTTTGCGTGTCTCGTCGTATACAGCGGCGGAGCCGGAAATAAAATTTGGATGGGCTATCGAGTTAAACTATATATATGA

Protein sequence

>Msa1327980

MVCTSAQRTNMIKRTGVKWYGVTKSTPSHITVSFFIIGHFFPSHCNNVILMCFFSFACLVVYSGGAGNKIWMGYRVKLYI*