AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1328040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 11 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGGAGCCGGAAATAAAATT+TGG 0.310961 8_4:-45721804 Msa1328040:CDS
GTCGTATACAGCGGCGGAGC+CGG 0.460313 8_4:-45721817 Msa1328040:CDS
CGTACTGGAGTTAAGTGGTA+TGG 0.485176 8_4:-45721955 Msa1328040:CDS
TAGAATATGATAAAACGTAC+TGG 0.488816 8_4:-45721970 Msa1328040:CDS
AATGAGACGGTGATATGGGA+TGG 0.537767 8_4:+45721916 None:intergenic
AGCCGGAAATAAAATTTGGA+TGG 0.541216 8_4:-45721800 Msa1328040:CDS
ATGACATTGTTACAATGTGA+TGG 0.550818 8_4:+45721871 None:intergenic
GCCGGAAATAAAATTTGGAT+GGG 0.580839 8_4:-45721799 Msa1328040:CDS
GACGGTGATATGGGATGGAG+TGG 0.602020 8_4:+45721921 None:intergenic
TAAAACGTACTGGAGTTAAG+TGG 0.625133 8_4:-45721960 Msa1328040:CDS
TGACATTGTTACAATGTGAT+GGG 0.627569 8_4:+45721872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTAGAAAATATAAAATA+TGG + chr8_4:45721861-45721880 None:intergenic 10.0%
!! AAAGTAGAAAATATAAAATA+TGG + chr8_4:45721861-45721880 None:intergenic 10.0%
!!! ATTAAGAATTTTTTAACTTC+TGG - chr8_4:45721829-45721848 Msa1328040:CDS 15.0%
!!! TTAAGAATTTTTTAACTTCT+GGG - chr8_4:45721830-45721849 Msa1328040:CDS 15.0%
!!! TCTACAAATGTATTTTATTT+AGG - chr8_4:45721880-45721899 Msa1328040:CDS 15.0%
!! AATCATATTATTTATGTCTA+TGG - chr8_4:45722736-45722755 Msa1328040:intron 15.0%
!!! ATTAAGAATTTTTTAACTTC+TGG - chr8_4:45721829-45721848 Msa1328040:CDS 15.0%
!!! TTAAGAATTTTTTAACTTCT+GGG - chr8_4:45721830-45721849 Msa1328040:CDS 15.0%
!!! TCTACAAATGTATTTTATTT+AGG - chr8_4:45721880-45721899 Msa1328040:CDS 15.0%
!! AATCATATTATTTATGTCTA+TGG - chr8_4:45722736-45722755 Msa1328040:intron 15.0%
!! AAATGAATGAAAATATAGAC+TGG - chr8_4:45721965-45721984 Msa1328040:CDS 20.0%
!!! CTATTTTCGTTTTTGTTAAT+GGG - chr8_4:45722559-45722578 Msa1328040:intron 20.0%
!!! TATTTTCGTTTTTGTTAATG+GGG - chr8_4:45722560-45722579 Msa1328040:intron 20.0%
!! ATAAACATAACATAAGCAAA+TGG + chr8_4:45722957-45722976 None:intergenic 20.0%
!! AGAAAATATCAGTTTCATTA+TGG + chr8_4:45723084-45723103 None:intergenic 20.0%
!! ACCAATTATAAAAAATGAGA+CGG + chr8_4:45723185-45723204 None:intergenic 20.0%
!! AAATGAATGAAAATATAGAC+TGG - chr8_4:45721965-45721984 Msa1328040:CDS 20.0%
!!! CTATTTTCGTTTTTGTTAAT+GGG - chr8_4:45722559-45722578 Msa1328040:intron 20.0%
!!! TATTTTCGTTTTTGTTAATG+GGG - chr8_4:45722560-45722579 Msa1328040:intron 20.0%
!! ATAAACATAACATAAGCAAA+TGG + chr8_4:45722957-45722976 None:intergenic 20.0%
!! AGAAAATATCAGTTTCATTA+TGG + chr8_4:45723084-45723103 None:intergenic 20.0%
!! ACCAATTATAAAAAATGAGA+CGG + chr8_4:45723185-45723204 None:intergenic 20.0%
! TAGGAAGTTATATTATGATG+TGG - chr8_4:45721899-45721918 Msa1328040:CDS 25.0%
!!! TCTTATTTCTGCTAACTAAT+AGG - chr8_4:45722145-45722164 Msa1328040:intron 25.0%
!!! CTTATTTCTGCTAACTAATA+GGG - chr8_4:45722146-45722165 Msa1328040:intron 25.0%
!!! TTATTTCTGCTAACTAATAG+GGG - chr8_4:45722147-45722166 Msa1328040:intron 25.0%
!! TACTCATAATTTGCAAGAAA+TGG + chr8_4:45722401-45722420 None:intergenic 25.0%
! AGCAAATATCTATAGAAGTA+TGG + chr8_4:45722478-45722497 None:intergenic 25.0%
!!! CTATAGATATTTGCTGATTT+AGG - chr8_4:45722483-45722502 Msa1328040:intron 25.0%
!!! CTGTTTTAAAGTTTTGTTCT+TGG - chr8_4:45722526-45722545 Msa1328040:intron 25.0%
! CCATTAACAAAAACGAAAAT+AGG + chr8_4:45722561-45722580 None:intergenic 25.0%
!!! CCTATTTTCGTTTTTGTTAA+TGG - chr8_4:45722558-45722577 Msa1328040:intron 25.0%
!!! TCAACTTACTTCTTTAGTTT+TGG - chr8_4:45722626-45722645 Msa1328040:intron 25.0%
! AGTTGTTCTATAATGCTAAA+TGG - chr8_4:45722673-45722692 Msa1328040:intron 25.0%
! TAATGTTAAGTTAATGTTGC+AGG - chr8_4:45722779-45722798 Msa1328040:intron 25.0%
!!! ACTTTGTTAGCTTATTGTTT+TGG - chr8_4:45722882-45722901 Msa1328040:intron 25.0%
!! CTGTATTTTGCATTTTATCA+AGG - chr8_4:45722915-45722934 Msa1328040:intron 25.0%
! TAGAATATGATAAAACGTAC+TGG - chr8_4:45723115-45723134 Msa1328040:intron 25.0%
!!! ACCGTCTCATTTTTTATAAT+TGG - chr8_4:45723181-45723200 Msa1328040:intron 25.0%
! TAGGAAGTTATATTATGATG+TGG - chr8_4:45721899-45721918 Msa1328040:CDS 25.0%
!!! TCTTATTTCTGCTAACTAAT+AGG - chr8_4:45722145-45722164 Msa1328040:intron 25.0%
!!! CTTATTTCTGCTAACTAATA+GGG - chr8_4:45722146-45722165 Msa1328040:intron 25.0%
!!! TTATTTCTGCTAACTAATAG+GGG - chr8_4:45722147-45722166 Msa1328040:intron 25.0%
!! TACTCATAATTTGCAAGAAA+TGG + chr8_4:45722401-45722420 None:intergenic 25.0%
! AGCAAATATCTATAGAAGTA+TGG + chr8_4:45722478-45722497 None:intergenic 25.0%
!!! CTATAGATATTTGCTGATTT+AGG - chr8_4:45722483-45722502 Msa1328040:intron 25.0%
!!! CTGTTTTAAAGTTTTGTTCT+TGG - chr8_4:45722526-45722545 Msa1328040:intron 25.0%
! CCATTAACAAAAACGAAAAT+AGG + chr8_4:45722561-45722580 None:intergenic 25.0%
!!! CCTATTTTCGTTTTTGTTAA+TGG - chr8_4:45722558-45722577 Msa1328040:intron 25.0%
!!! TCAACTTACTTCTTTAGTTT+TGG - chr8_4:45722626-45722645 Msa1328040:intron 25.0%
! AGTTGTTCTATAATGCTAAA+TGG - chr8_4:45722673-45722692 Msa1328040:intron 25.0%
! TAATGTTAAGTTAATGTTGC+AGG - chr8_4:45722779-45722798 Msa1328040:intron 25.0%
!!! ACTTTGTTAGCTTATTGTTT+TGG - chr8_4:45722882-45722901 Msa1328040:intron 25.0%
!! CTGTATTTTGCATTTTATCA+AGG - chr8_4:45722915-45722934 Msa1328040:intron 25.0%
! TAGAATATGATAAAACGTAC+TGG - chr8_4:45723115-45723134 Msa1328040:intron 25.0%
!!! ACCGTCTCATTTTTTATAAT+TGG - chr8_4:45723181-45723200 Msa1328040:intron 25.0%
TTTATTCCTTTCCCATAAGA+TGG + chr8_4:45721789-45721808 None:intergenic 30.0%
! TATCAGTAAGATTTGGTCAA+TGG + chr8_4:45721928-45721947 None:intergenic 30.0%
ATACGAGTTGTTTATTGTAG+AGG - chr8_4:45722013-45722032 Msa1328040:intron 30.0%
ACTTAGAAATTGTATCAGTC+TGG + chr8_4:45722049-45722068 None:intergenic 30.0%
! TATTTCTGCTAACTAATAGG+GGG - chr8_4:45722148-45722167 Msa1328040:intron 30.0%
! CATATCGCTTACAATTTGTT+TGG - chr8_4:45722177-45722196 Msa1328040:intron 30.0%
!! GAGAATGTCATTGATTTAGT+TGG + chr8_4:45722608-45722627 None:intergenic 30.0%
! CTTGTTGGTTACAAATTTGA+CGG - chr8_4:45722851-45722870 Msa1328040:intron 30.0%
AAAAAATGAGACGGTGATAT+GGG + chr8_4:45723176-45723195 None:intergenic 30.0%
TAAAAAATGAGACGGTGATA+TGG + chr8_4:45723177-45723196 None:intergenic 30.0%
TGACATTGTTACAATGTGAT+GGG + chr8_4:45723216-45723235 None:intergenic 30.0%
ATGACATTGTTACAATGTGA+TGG + chr8_4:45723217-45723236 None:intergenic 30.0%
TTTATTCCTTTCCCATAAGA+TGG + chr8_4:45721789-45721808 None:intergenic 30.0%
! TATCAGTAAGATTTGGTCAA+TGG + chr8_4:45721928-45721947 None:intergenic 30.0%
ATACGAGTTGTTTATTGTAG+AGG - chr8_4:45722013-45722032 Msa1328040:intron 30.0%
ACTTAGAAATTGTATCAGTC+TGG + chr8_4:45722049-45722068 None:intergenic 30.0%
! TATTTCTGCTAACTAATAGG+GGG - chr8_4:45722148-45722167 Msa1328040:intron 30.0%
! CATATCGCTTACAATTTGTT+TGG - chr8_4:45722177-45722196 Msa1328040:intron 30.0%
!! GAGAATGTCATTGATTTAGT+TGG + chr8_4:45722608-45722627 None:intergenic 30.0%
! CTTGTTGGTTACAAATTTGA+CGG - chr8_4:45722851-45722870 Msa1328040:intron 30.0%
AAAAAATGAGACGGTGATAT+GGG + chr8_4:45723176-45723195 None:intergenic 30.0%
TAAAAAATGAGACGGTGATA+TGG + chr8_4:45723177-45723196 None:intergenic 30.0%
TGACATTGTTACAATGTGAT+GGG + chr8_4:45723216-45723235 None:intergenic 30.0%
ATGACATTGTTACAATGTGA+TGG + chr8_4:45723217-45723236 None:intergenic 30.0%
! ATGGTAGTGTTACCATCTTA+TGG - chr8_4:45721774-45721793 Msa1328040:CDS 35.0%
! TGGTAGTGTTACCATCTTAT+GGG - chr8_4:45721775-45721794 Msa1328040:CDS 35.0%
TGACCAAATCTTACTGATAG+AGG - chr8_4:45721929-45721948 Msa1328040:CDS 35.0%
GACCAAATCTTACTGATAGA+GGG - chr8_4:45721930-45721949 Msa1328040:CDS 35.0%
AACCCTCTATCAGTAAGATT+TGG + chr8_4:45721935-45721954 None:intergenic 35.0%
AAATTGTATCAGTCTGGTGA+AGG + chr8_4:45722043-45722062 None:intergenic 35.0%
TGTTAGTGACGTTATAAGGA+TGG + chr8_4:45722093-45722112 None:intergenic 35.0%
CTTGTGTTAGTGACGTTATA+AGG + chr8_4:45722097-45722116 None:intergenic 35.0%
AGAACGAGTTTCATCATCTA+TGG - chr8_4:45722119-45722138 Msa1328040:intron 35.0%
!! TGGCTCAGATGATTTTGAAA+TGG + chr8_4:45722458-45722477 None:intergenic 35.0%
AGGATGAATCAGTGTAGATT+TGG - chr8_4:45722799-45722818 Msa1328040:intron 35.0%
GGTTATTCAATCTTCCTTGT+TGG - chr8_4:45722836-45722855 Msa1328040:intron 35.0%
CGTCAAATTTGTAACCAACA+AGG + chr8_4:45722853-45722872 None:intergenic 35.0%
GTTTATCTTGTGTAGAACAG+AGG - chr8_4:45722971-45722990 Msa1328040:intron 35.0%
GCTATAACTCTGTAAAGTTG+TGG - chr8_4:45723044-45723063 Msa1328040:intron 35.0%
TAAAACGTACTGGAGTTAAG+TGG - chr8_4:45723125-45723144 Msa1328040:intron 35.0%
AGCCGGAAATAAAATTTGGA+TGG - chr8_4:45723285-45723304 Msa1328040:intron 35.0%
GCCGGAAATAAAATTTGGAT+GGG - chr8_4:45723286-45723305 Msa1328040:intron 35.0%
!! GCCCATCCAAATTTTATTTC+CGG + chr8_4:45723290-45723309 None:intergenic 35.0%
! ATGGTAGTGTTACCATCTTA+TGG - chr8_4:45721774-45721793 Msa1328040:CDS 35.0%
! TGGTAGTGTTACCATCTTAT+GGG - chr8_4:45721775-45721794 Msa1328040:CDS 35.0%
TGACCAAATCTTACTGATAG+AGG - chr8_4:45721929-45721948 Msa1328040:CDS 35.0%
GACCAAATCTTACTGATAGA+GGG - chr8_4:45721930-45721949 Msa1328040:CDS 35.0%
AACCCTCTATCAGTAAGATT+TGG + chr8_4:45721935-45721954 None:intergenic 35.0%
AAATTGTATCAGTCTGGTGA+AGG + chr8_4:45722043-45722062 None:intergenic 35.0%
TGTTAGTGACGTTATAAGGA+TGG + chr8_4:45722093-45722112 None:intergenic 35.0%
CTTGTGTTAGTGACGTTATA+AGG + chr8_4:45722097-45722116 None:intergenic 35.0%
AGAACGAGTTTCATCATCTA+TGG - chr8_4:45722119-45722138 Msa1328040:intron 35.0%
!! TGGCTCAGATGATTTTGAAA+TGG + chr8_4:45722458-45722477 None:intergenic 35.0%
AGGATGAATCAGTGTAGATT+TGG - chr8_4:45722799-45722818 Msa1328040:intron 35.0%
GGTTATTCAATCTTCCTTGT+TGG - chr8_4:45722836-45722855 Msa1328040:intron 35.0%
CGTCAAATTTGTAACCAACA+AGG + chr8_4:45722853-45722872 None:intergenic 35.0%
GTTTATCTTGTGTAGAACAG+AGG - chr8_4:45722971-45722990 Msa1328040:intron 35.0%
GCTATAACTCTGTAAAGTTG+TGG - chr8_4:45723044-45723063 Msa1328040:intron 35.0%
TAAAACGTACTGGAGTTAAG+TGG - chr8_4:45723125-45723144 Msa1328040:intron 35.0%
AGCCGGAAATAAAATTTGGA+TGG - chr8_4:45723285-45723304 Msa1328040:intron 35.0%
GCCGGAAATAAAATTTGGAT+GGG - chr8_4:45723286-45723305 Msa1328040:intron 35.0%
!! GCCCATCCAAATTTTATTTC+CGG + chr8_4:45723290-45723309 None:intergenic 35.0%
GTGTTACCATCTTATGGGAA+AGG - chr8_4:45721780-45721799 Msa1328040:CDS 40.0%
TGACGTTATAAGGATGGTGA+GGG + chr8_4:45722087-45722106 None:intergenic 40.0%
CAACACTGTCTACATCAAGA+GGG + chr8_4:45722218-45722237 None:intergenic 40.0%
GATTTGGCATTTGCACATTC+TGG - chr8_4:45722815-45722834 Msa1328040:intron 40.0%
GTGTTACCATCTTATGGGAA+AGG - chr8_4:45721780-45721799 Msa1328040:CDS 40.0%
TGACGTTATAAGGATGGTGA+GGG + chr8_4:45722087-45722106 None:intergenic 40.0%
CAACACTGTCTACATCAAGA+GGG + chr8_4:45722218-45722237 None:intergenic 40.0%
GATTTGGCATTTGCACATTC+TGG - chr8_4:45722815-45722834 Msa1328040:intron 40.0%
TTGTATCAGTCTGGTGAAGG+AGG + chr8_4:45722040-45722059 None:intergenic 45.0%
GACGTTATAAGGATGGTGAG+GGG + chr8_4:45722086-45722105 None:intergenic 45.0%
GTGACGTTATAAGGATGGTG+AGG + chr8_4:45722088-45722107 None:intergenic 45.0%
GCAACACTGTCTACATCAAG+AGG + chr8_4:45722219-45722238 None:intergenic 45.0%
CGTACTGGAGTTAAGTGGTA+TGG - chr8_4:45723130-45723149 Msa1328040:intron 45.0%
AATGAGACGGTGATATGGGA+TGG + chr8_4:45723172-45723191 None:intergenic 45.0%
GCGGAGCCGGAAATAAAATT+TGG - chr8_4:45723281-45723300 Msa1328040:intron 45.0%
TTGTATCAGTCTGGTGAAGG+AGG + chr8_4:45722040-45722059 None:intergenic 45.0%
GACGTTATAAGGATGGTGAG+GGG + chr8_4:45722086-45722105 None:intergenic 45.0%
GTGACGTTATAAGGATGGTG+AGG + chr8_4:45722088-45722107 None:intergenic 45.0%
GCAACACTGTCTACATCAAG+AGG + chr8_4:45722219-45722238 None:intergenic 45.0%
CGTACTGGAGTTAAGTGGTA+TGG - chr8_4:45723130-45723149 Msa1328040:intron 45.0%
AATGAGACGGTGATATGGGA+TGG + chr8_4:45723172-45723191 None:intergenic 45.0%
GCGGAGCCGGAAATAAAATT+TGG - chr8_4:45723281-45723300 Msa1328040:intron 45.0%
GACGGTGATATGGGATGGAG+TGG + chr8_4:45723167-45723186 None:intergenic 55.0%
GCGTGTCTCGTCGTATACAG+CGG - chr8_4:45723259-45723278 Msa1328040:intron 55.0%
TGTCTCGTCGTATACAGCGG+CGG - chr8_4:45723262-45723281 Msa1328040:intron 55.0%
GACGGTGATATGGGATGGAG+TGG + chr8_4:45723167-45723186 None:intergenic 55.0%
GCGTGTCTCGTCGTATACAG+CGG - chr8_4:45723259-45723278 Msa1328040:intron 55.0%
TGTCTCGTCGTATACAGCGG+CGG - chr8_4:45723262-45723281 Msa1328040:intron 55.0%
GGATGGTGAGGGGCAGAGTA+AGG + chr8_4:45722076-45722095 None:intergenic 60.0%
GGATGGTGAGGGGCAGAGTA+AGG + chr8_4:45722076-45722095 None:intergenic 60.0%
GTCGTATACAGCGGCGGAGC+CGG - chr8_4:45723268-45723287 Msa1328040:intron 65.0%
GTCGTATACAGCGGCGGAGC+CGG - chr8_4:45723268-45723287 Msa1328040:intron 65.0%
Chromosome Type Strat End Strand Name
chr8_4 gene 45721774 45723333 45721774 ID=Msa1328040;Name=Msa1328040
chr8_4 mRNA 45721774 45723333 45721774 ID=Msa1328040-mRNA-1;Parent=Msa1328040;Name=Msa1328040-mRNA-1;_AED=0.94;_eAED=0.94;_QI=0|0.25|0|0.8|1|1|5|0|80
chr8_4 exon 45723328 45723333 45723328 ID=Msa1328040-mRNA-1:exon:12858;Parent=Msa1328040-mRNA-1
chr8_4 exon 45722191 45722199 45722191 ID=Msa1328040-mRNA-1:exon:12857;Parent=Msa1328040-mRNA-1
chr8_4 exon 45722165 45722170 45722165 ID=Msa1328040-mRNA-1:exon:12856;Parent=Msa1328040-mRNA-1
chr8_4 exon 45722111 45722116 45722111 ID=Msa1328040-mRNA-1:exon:12855;Parent=Msa1328040-mRNA-1
chr8_4 exon 45721774 45721989 45721774 ID=Msa1328040-mRNA-1:exon:12854;Parent=Msa1328040-mRNA-1
chr8_4 CDS 45723328 45723333 45723328 ID=Msa1328040-mRNA-1:cds;Parent=Msa1328040-mRNA-1
chr8_4 CDS 45722191 45722199 45722191 ID=Msa1328040-mRNA-1:cds;Parent=Msa1328040-mRNA-1
chr8_4 CDS 45722165 45722170 45722165 ID=Msa1328040-mRNA-1:cds;Parent=Msa1328040-mRNA-1
chr8_4 CDS 45722111 45722116 45722111 ID=Msa1328040-mRNA-1:cds;Parent=Msa1328040-mRNA-1
chr8_4 CDS 45721774 45721989 45721774 ID=Msa1328040-mRNA-1:cds;Parent=Msa1328040-mRNA-1
Gene Sequence

>Msa1328040

ATGGTATGTACTTCTGCTCAAAGGACAAATATGATAAAACGTACTGGAGTTAAGTGGTATGGAGTAACAAAATCCACTCCATCCCATATCACCGTCTCATTTTTTATAATTGGTCATTTTTTCCCATCACATTGTAACAATGTCATTCTCATGTGTTTCTTCTCTTTTGCGTGTCTCGTCGTATACAGCGGCGGAGCCGGAAATAAAATTTGGATGGGCTATCGAGTTAAACTATATATATGA

Protein sequence

>Msa1328040

MVCTSAQRTNMIKRTGVKWYGVTKSTPSHITVSFFIIGHFFPSHCNNVILMCFFSFACLVVYSGGAGNKIWMGYRVKLYI*