Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1351080 | Msa1359820 | 0.806151 | 9.453270e-50 | -8.615850e-47 |
| Msa1351080 | Msa1444370 | 0.806853 | 6.722895e-50 | -8.615850e-47 |
| Msa0629850 | Msa1351080 | 0.814472 | 1.514132e-51 | -8.615850e-47 |
| Msa0629930 | Msa1351080 | 0.817623 | 2.995699e-52 | -8.615850e-47 |
| Msa0629940 | Msa1351080 | 0.810724 | 9.996187e-51 | -8.615850e-47 |
| Msa0782080 | Msa1351080 | 0.806364 | 8.527379e-50 | -8.615850e-47 |
| Msa0818030 | Msa1351080 | 0.801906 | 7.219515e-49 | -8.615850e-47 |
| Msa1287560 | Msa1351080 | 0.810061 | 1.390182e-50 | -8.615850e-47 |
| Msa1288000 | Msa1351080 | 0.815433 | 9.268917e-52 | -8.615850e-47 |
| Msa0443750 | Msa1351080 | 0.827329 | 1.661748e-54 | -8.615850e-47 |
| Msa1006250 | Msa1351080 | 0.827637 | 1.401600e-54 | -8.615850e-47 |
| Msa1050480 | Msa1351080 | 0.809139 | 2.192841e-50 | -8.615850e-47 |
| Msa0362850 | Msa1351080 | 0.801497 | 8.755537e-49 | -8.615850e-47 |
| Msa0533290 | Msa1351080 | 0.836181 | 1.087784e-56 | -8.615850e-47 |
| Msa0533430 | Msa1351080 | 0.814773 | 1.298482e-51 | -8.615850e-47 |
| Msa0858360 | Msa1351080 | 0.825247 | 5.202359e-54 | -8.615850e-47 |
| Msa0902020 | Msa1351080 | 0.830553 | 2.752497e-55 | -8.615850e-47 |
| Msa0912370 | Msa1351080 | 0.803579 | 3.259308e-49 | -8.615850e-47 |
| Msa0924370 | Msa1351080 | 0.803550 | 3.305171e-49 | -8.615850e-47 |
| Msa0963310 | Msa1351080 | 0.842144 | 3.092875e-58 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAAGTGATGCTTGTTATT+TGG | 0.173222 | tig0012121:+3636 | Msa1351080:CDS |
| TCCCCTGCCATCGTCAGATT+TGG | 0.324141 | tig0012121:-3785 | None:intergenic |
| GCTAAGGTTTCCACGTGAAT+AGG | 0.359375 | tig0012121:-3575 | None:intergenic |
| TGCTGAAAACAATTATCTAC+TGG | 0.385508 | tig0012121:+3834 | Msa1351080:CDS |
| TTTCCAAATCTGACGATGGC+AGG | 0.440047 | tig0012121:+3782 | Msa1351080:CDS |
| TCTGACGATGGCAGGGGACG+AGG | 0.475481 | tig0012121:+3790 | Msa1351080:CDS |
| TGTGCCACATACAAACCTTC+CGG | 0.476709 | tig0012121:-3889 | None:intergenic |
| TTCCCTGAAAAGCTAGAATC+CGG | 0.512868 | tig0012121:-3916 | None:intergenic |
| AATTGTACCACGCATTGCTA+AGG | 0.531736 | tig0012121:-3591 | None:intergenic |
| TTCCAAATCTGACGATGGCA+GGG | 0.553847 | tig0012121:+3783 | Msa1351080:CDS |
| ATAAACTTGTTCAGAAACTC+CGG | 0.563498 | tig0012121:+3870 | Msa1351080:CDS |
| TCCAAATCTGACGATGGCAG+GGG | 0.572024 | tig0012121:+3784 | Msa1351080:CDS |
| TGCTTCTCGTCCTATTCACG+TGG | 0.599877 | tig0012121:+3565 | Msa1351080:CDS |
| CTGACGATGGCAGGGGACGA+GGG | 0.613499 | tig0012121:+3791 | Msa1351080:CDS |
| ACTTGTTCAGAAACTCCGGA+AGG | 0.630459 | tig0012121:+3874 | Msa1351080:CDS |
| AACTCCGGAAGGTTTGTATG+TGG | 0.633121 | tig0012121:+3885 | Msa1351080:CDS |
| GATGTTTCCAAATCTGACGA+TGG | 0.663090 | tig0012121:+3778 | Msa1351080:CDS |
| GTGGAAACCTTAGCAATGCG+TGG | 0.686228 | tig0012121:+3584 | Msa1351080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAATTATTAGGAAAATAAAA+AGG | - | tig0012121:3697-3716 | None:intergenic | 15.0% |
| !!! | AAATAGAAGCATGAATTATT+AGG | - | tig0012121:3709-3728 | None:intergenic | 20.0% |
| !! | TACAAGTGATGCTTGTTATT+TGG | + | tig0012121:3636-3655 | Msa1351080:CDS | 30.0% |
| ! | CTATTTCTTGATGTTCAACA+AGG | + | tig0012121:3723-3742 | Msa1351080:intron | 30.0% |
| TGCTGAAAACAATTATCTAC+TGG | + | tig0012121:3834-3853 | Msa1351080:CDS | 30.0% | |
| ! | ATAAACTTGTTCAGAAACTC+CGG | + | tig0012121:3870-3889 | Msa1351080:CDS | 30.0% |
| ! | GGAAACACAATTTTGTATGT+AGG | + | tig0012121:3935-3954 | Msa1351080:CDS | 30.0% |
| ! | TTTGTATGTGGCACATTTTC+CGG | + | tig0012121:3897-3916 | Msa1351080:CDS | 35.0% |
| AATTGTACCACGCATTGCTA+AGG | - | tig0012121:3594-3613 | None:intergenic | 40.0% | |
| GATGTTTCCAAATCTGACGA+TGG | + | tig0012121:3778-3797 | Msa1351080:CDS | 40.0% | |
| !!! | TTTCCGGATTCTAGCTTTTC+AGG | + | tig0012121:3913-3932 | Msa1351080:CDS | 40.0% |
| !!! | TTCCGGATTCTAGCTTTTCA+GGG | + | tig0012121:3914-3933 | Msa1351080:CDS | 40.0% |
| TTCCCTGAAAAGCTAGAATC+CGG | - | tig0012121:3919-3938 | None:intergenic | 40.0% | |
| GCTAAGGTTTCCACGTGAAT+AGG | - | tig0012121:3578-3597 | None:intergenic | 45.0% | |
| TTTCCAAATCTGACGATGGC+AGG | + | tig0012121:3782-3801 | Msa1351080:CDS | 45.0% | |
| TTCCAAATCTGACGATGGCA+GGG | + | tig0012121:3783-3802 | Msa1351080:CDS | 45.0% | |
| ACTTGTTCAGAAACTCCGGA+AGG | + | tig0012121:3874-3893 | Msa1351080:CDS | 45.0% | |
| AACTCCGGAAGGTTTGTATG+TGG | + | tig0012121:3885-3904 | Msa1351080:CDS | 45.0% | |
| TGTGCCACATACAAACCTTC+CGG | - | tig0012121:3892-3911 | None:intergenic | 45.0% | |
| TGCTTCTCGTCCTATTCACG+TGG | + | tig0012121:3565-3584 | Msa1351080:CDS | 50.0% | |
| GTGGAAACCTTAGCAATGCG+TGG | + | tig0012121:3584-3603 | Msa1351080:CDS | 50.0% | |
| TCCAAATCTGACGATGGCAG+GGG | + | tig0012121:3784-3803 | Msa1351080:CDS | 50.0% | |
| TCCCCTGCCATCGTCAGATT+TGG | - | tig0012121:3788-3807 | None:intergenic | 55.0% | |
| TCTGACGATGGCAGGGGACG+AGG | + | tig0012121:3790-3809 | Msa1351080:CDS | 65.0% | |
| CTGACGATGGCAGGGGACGA+GGG | + | tig0012121:3791-3810 | Msa1351080:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0012121 | gene | 3560 | 3988 | 3560 | ID=Msa1351080;Name=Msa1351080 |
| tig0012121 | mRNA | 3560 | 3988 | 3560 | ID=Msa1351080-mRNA-1;Parent=Msa1351080;Name=Msa1351080-mRNA-1;_AED=0.44;_eAED=0.59;_QI=0|0|0|1|1|1|2|0|113 |
| tig0012121 | exon | 3560 | 3657 | 3560 | ID=Msa1351080-mRNA-1:exon:7654;Parent=Msa1351080-mRNA-1 |
| tig0012121 | exon | 3745 | 3988 | 3745 | ID=Msa1351080-mRNA-1:exon:7655;Parent=Msa1351080-mRNA-1 |
| tig0012121 | CDS | 3560 | 3657 | 3560 | ID=Msa1351080-mRNA-1:cds;Parent=Msa1351080-mRNA-1 |
| tig0012121 | CDS | 3745 | 3988 | 3745 | ID=Msa1351080-mRNA-1:cds;Parent=Msa1351080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
