Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1050480 | RHN44458.1 | 67.500 | 80 | 20 | 1 | 1 | 80 | 1785 | 1858 | 5.68e-23 | 102 |
| Msa1050480 | RHN44458.1 | 86.250 | 80 | 11 | 0 | 1 | 80 | 789 | 868 | 1.97e-36 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1050480 | A0A396GVJ4 | 67.500 | 80 | 20 | 1 | 1 | 80 | 1785 | 1858 | 2.71e-23 | 102 |
| Msa1050480 | A0A396GVJ4 | 86.250 | 80 | 11 | 0 | 1 | 80 | 789 | 868 | 9.39e-37 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0021460 | Msa1050480 | 0.806731 | 7.134452e-50 | -8.615850e-47 |
| Msa0032290 | Msa1050480 | 0.802581 | 5.242667e-49 | -8.615850e-47 |
| Msa0088550 | Msa1050480 | 0.824638 | 7.242202e-54 | -8.615850e-47 |
| Msa0109970 | Msa1050480 | 0.816041 | 6.785077e-52 | -8.615850e-47 |
| Msa0109990 | Msa1050480 | 0.813897 | 2.028029e-51 | -8.615850e-47 |
| Msa0617370 | Msa1050480 | 0.808609 | 2.847741e-50 | -8.615850e-47 |
| Msa0618430 | Msa1050480 | 0.805570 | 1.252471e-49 | -8.615850e-47 |
| Msa0629850 | Msa1050480 | 0.800878 | 1.172098e-48 | -8.615850e-47 |
| Msa0629890 | Msa1050480 | 0.841610 | 4.279636e-58 | -8.615850e-47 |
| Msa0629930 | Msa1050480 | 0.832745 | 7.934210e-56 | -8.615850e-47 |
| Msa0629940 | Msa1050480 | 0.828265 | 9.897790e-55 | -8.615850e-47 |
| Msa0632540 | Msa1050480 | 0.811223 | 7.796044e-51 | -8.615850e-47 |
| Msa0672810 | Msa1050480 | 0.831726 | 1.417514e-55 | -8.615850e-47 |
| Msa0672850 | Msa1050480 | 0.804940 | 1.696998e-49 | -8.615850e-47 |
| Msa0678240 | Msa1050480 | 0.800659 | 1.298935e-48 | -8.615850e-47 |
| Msa0752580 | Msa1050480 | 0.835689 | 1.449326e-56 | -8.615850e-47 |
| Msa0754180 | Msa1050480 | 0.830012 | 3.731773e-55 | -8.615850e-47 |
| Msa0754500 | Msa1050480 | 0.826940 | 2.058839e-54 | -8.615850e-47 |
| Msa0773610 | Msa1050480 | 0.830302 | 3.171108e-55 | -8.615850e-47 |
| Msa0784580 | Msa1050480 | 0.824774 | 6.725980e-54 | -8.615850e-47 |
| Msa0811000 | Msa1050480 | 0.820636 | 6.176945e-53 | -8.615850e-47 |
| Msa0818010 | Msa1050480 | 0.801606 | 8.316432e-49 | -8.615850e-47 |
| Msa0818030 | Msa1050480 | 0.845103 | 5.004059e-59 | -8.615850e-47 |
| Msa0819740 | Msa1050480 | 0.800307 | 1.532545e-48 | -8.615850e-47 |
| Msa0830900 | Msa1050480 | 0.822113 | 2.818815e-53 | -8.615850e-47 |
| Msa0135360 | Msa1050480 | 0.821890 | 3.173598e-53 | -8.615850e-47 |
| Msa0137330 | Msa1050480 | 0.802871 | 4.566770e-49 | -8.615850e-47 |
| Msa0142230 | Msa1050480 | 0.827664 | 1.380392e-54 | -8.615850e-47 |
| Msa0151860 | Msa1050480 | 0.810568 | 1.080492e-50 | -8.615850e-47 |
| Msa0153430 | Msa1050480 | 0.836560 | 8.710574e-57 | -8.615850e-47 |
| Msa0153440 | Msa1050480 | 0.826629 | 2.442912e-54 | -8.615850e-47 |
| Msa0155110 | Msa1050480 | 0.800937 | 1.140239e-48 | -8.615850e-47 |
| Msa0156170 | Msa1050480 | 0.803126 | 4.044795e-49 | -8.615850e-47 |
| Msa0172440 | Msa1050480 | 0.804201 | 2.420024e-49 | -8.615850e-47 |
| Msa0179860 | Msa1050480 | 0.819156 | 1.346776e-52 | -8.615850e-47 |
| Msa0193010 | Msa1050480 | 0.824493 | 7.834176e-54 | -8.615850e-47 |
| Msa0193760 | Msa1050480 | 0.820202 | 7.769183e-53 | -8.615850e-47 |
| Msa0199730 | Msa1050480 | 0.805982 | 1.026145e-49 | -8.615850e-47 |
| Msa0203070 | Msa1050480 | 0.833962 | 3.945304e-56 | -8.615850e-47 |
| Msa0211670 | Msa1050480 | 0.827202 | 1.781789e-54 | -8.615850e-47 |
| Msa0232860 | Msa1050480 | 0.839374 | 1.645860e-57 | -8.615850e-47 |
| Msa0235550 | Msa1050480 | 0.830307 | 3.161304e-55 | -8.615850e-47 |
| Msa0389050 | Msa1050480 | 0.845497 | 3.915614e-59 | -8.615850e-47 |
| Msa0389310 | Msa1050480 | 0.805386 | 1.369035e-49 | -8.615850e-47 |
| Msa0391080 | Msa1050480 | 0.802690 | 4.978548e-49 | -8.615850e-47 |
| Msa0409820 | Msa1050480 | 0.820043 | 8.446787e-53 | -8.615850e-47 |
| Msa0414070 | Msa1050480 | 0.803418 | 3.519637e-49 | -8.615850e-47 |
| Msa0439700 | Msa1050480 | 0.818395 | 2.004535e-52 | -8.615850e-47 |
| Msa0452410 | Msa1050480 | 0.805918 | 1.058245e-49 | -8.615850e-47 |
| Msa0452480 | Msa1050480 | 0.808497 | 3.008722e-50 | -8.615850e-47 |
| Msa0455260 | Msa1050480 | 0.827410 | 1.588827e-54 | -8.615850e-47 |
| Msa1002300 | Msa1050480 | 0.803529 | 3.337382e-49 | -8.615850e-47 |
| Msa1006250 | Msa1050480 | 0.803119 | 4.058534e-49 | -8.615850e-47 |
| Msa1006400 | Msa1050480 | 0.802960 | 4.377505e-49 | -8.615850e-47 |
| Msa1006680 | Msa1050480 | 0.824176 | 9.302845e-54 | -8.615850e-47 |
| Msa1009170 | Msa1050480 | 0.806200 | 9.232437e-50 | -8.615850e-47 |
| Msa1009190 | Msa1050480 | 0.818353 | 2.048624e-52 | -8.615850e-47 |
| Msa1009200 | Msa1050480 | 0.831231 | 1.876674e-55 | -8.615850e-47 |
| Msa1020720 | Msa1050480 | 0.811527 | 6.697249e-51 | -8.615850e-47 |
| Msa1027240 | Msa1050480 | 0.814215 | 1.726134e-51 | -8.615850e-47 |
| Msa1037580 | Msa1050480 | 0.823915 | 1.071047e-53 | -8.615850e-47 |
| Msa1048490 | Msa1050480 | 0.834165 | 3.509312e-56 | -8.615850e-47 |
| Msa1048590 | Msa1050480 | 0.835098 | 2.044744e-56 | -8.615850e-47 |
| Msa1048610 | Msa1050480 | 0.835055 | 2.096717e-56 | -8.615850e-47 |
| Msa1050480 | Msa1050490 | 0.857666 | 1.397401e-62 | -8.615850e-47 |
| Msa1050480 | Msa1050500 | 0.877455 | 6.201138e-69 | -8.615850e-47 |
| Msa1050480 | Msa1052070 | 0.809246 | 2.080867e-50 | -8.615850e-47 |
| Msa1050480 | Msa1100180 | 0.814805 | 1.278024e-51 | -8.615850e-47 |
| Msa1050480 | Msa1135660 | 0.846101 | 2.683944e-59 | -8.615850e-47 |
| Msa1050480 | Msa1138450 | 0.814188 | 1.749635e-51 | -8.615850e-47 |
| Msa1050480 | Msa1138480 | 0.839059 | 1.987229e-57 | -8.615850e-47 |
| Msa1050480 | Msa1139240 | 0.821595 | 3.713768e-53 | -8.615850e-47 |
| Msa1050480 | Msa1167000 | 0.800004 | 1.766009e-48 | -8.615850e-47 |
| Msa1050480 | Msa1182830 | 0.818080 | 2.362418e-52 | -8.615850e-47 |
| Msa1050480 | Msa1258280 | 0.813445 | 2.550921e-51 | -8.615850e-47 |
| Msa1050480 | Msa1279850 | 0.845805 | 3.228522e-59 | -8.615850e-47 |
| Msa1050480 | Msa1279860 | 0.839172 | 1.857440e-57 | -8.615850e-47 |
| Msa1050480 | Msa1279870 | 0.810185 | 1.307143e-50 | -8.615850e-47 |
| Msa1050480 | Msa1285830 | 0.842076 | 3.224686e-58 | -8.615850e-47 |
| Msa1050480 | Msa1287560 | 0.848996 | 4.292683e-60 | -8.615850e-47 |
| Msa1050480 | Msa1287700 | 0.841749 | 3.934902e-58 | -8.615850e-47 |
| Msa1050480 | Msa1288000 | 0.816713 | 4.797998e-52 | -8.615850e-47 |
| Msa1050480 | Msa1292380 | 0.802730 | 4.883221e-49 | -8.615850e-47 |
| Msa1050480 | Msa1298290 | 0.823179 | 1.592525e-53 | -8.615850e-47 |
| Msa1050480 | Msa1351080 | 0.809139 | 2.192841e-50 | -8.615850e-47 |
| Msa1050480 | Msa1359820 | 0.803971 | 2.702117e-49 | -8.615850e-47 |
| Msa1050480 | Msa1384320 | 0.806604 | 7.587547e-50 | -8.615850e-47 |
| Msa1050480 | Msa1406600 | 0.813315 | 2.723236e-51 | -8.615850e-47 |
| Msa1050480 | Msa1423970 | 0.804286 | 2.323462e-49 | -8.615850e-47 |
| Msa1050480 | Msa1423990 | 0.813730 | 2.207122e-51 | -8.615850e-47 |
| Msa1050480 | Msa1447440 | 0.808535 | 2.952582e-50 | -8.615850e-47 |
| Msa0254190 | Msa1050480 | 0.801091 | 1.060388e-48 | -8.615850e-47 |
| Msa0256270 | Msa1050480 | 0.807339 | 5.305907e-50 | -8.615850e-47 |
| Msa0273570 | Msa1050480 | 0.827736 | 1.326877e-54 | -8.615850e-47 |
| Msa0274520 | Msa1050480 | 0.816664 | 4.920965e-52 | -8.615850e-47 |
| Msa0284810 | Msa1050480 | 0.854830 | 9.478610e-62 | -8.615850e-47 |
| Msa0323550 | Msa1050480 | 0.846502 | 2.086450e-59 | -8.615850e-47 |
| Msa0329550 | Msa1050480 | 0.802681 | 4.998626e-49 | -8.615850e-47 |
| Msa0331430 | Msa1050480 | 0.801396 | 9.182553e-49 | -8.615850e-47 |
| Msa0352010 | Msa1050480 | 0.806138 | 9.515736e-50 | -8.615850e-47 |
| Msa0363440 | Msa1050480 | 0.818564 | 1.835062e-52 | -8.615850e-47 |
| Msa0499710 | Msa1050480 | 0.818280 | 2.128457e-52 | -8.615850e-47 |
| Msa0530930 | Msa1050480 | 0.800418 | 1.455061e-48 | -8.615850e-47 |
| Msa0533290 | Msa1050480 | 0.808756 | 2.648363e-50 | -8.615850e-47 |
| Msa0587670 | Msa1050480 | 0.806135 | 9.527773e-50 | -8.615850e-47 |
| Msa0592470 | Msa1050480 | 0.803093 | 4.109502e-49 | -8.615850e-47 |
| Msa0854620 | Msa1050480 | 0.807329 | 5.329844e-50 | -8.615850e-47 |
| Msa0858360 | Msa1050480 | 0.829789 | 4.228125e-55 | -8.615850e-47 |
| Msa0862020 | Msa1050480 | 0.840138 | 1.041354e-57 | -8.615850e-47 |
| Msa0883450 | Msa1050480 | 0.800297 | 1.539559e-48 | -8.615850e-47 |
| Msa0884920 | Msa1050480 | 0.801529 | 8.623571e-49 | -8.615850e-47 |
| Msa0885170 | Msa1050480 | 0.804583 | 2.015284e-49 | -8.615850e-47 |
| Msa0898370 | Msa1050480 | 0.809662 | 1.693513e-50 | -8.615850e-47 |
| Msa0902980 | Msa1050480 | 0.806552 | 7.781896e-50 | -8.615850e-47 |
| Msa0951720 | Msa1050480 | 0.835989 | 1.217160e-56 | -8.615850e-47 |
| Msa0972240 | Msa1050480 | 0.817516 | 3.167373e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1050480 | MtrunA17_Chr7g0219651 | 86.250 | 80 | 11 | 0 | 1 | 80 | 789 | 868 | 1.81e-40 | 140 |
| Msa1050480 | MtrunA17_Chr7g0219651 | 67.500 | 80 | 20 | 1 | 1 | 80 | 1785 | 1858 | 5.23e-27 | 102 |
| Msa1050480 | MtrunA17_Chr7g0219581 | 72.289 | 83 | 20 | 1 | 1 | 80 | 224 | 306 | 6.13e-35 | 120 |
| Msa1050480 | MtrunA17_Chr7g0219601 | 72.289 | 83 | 20 | 1 | 1 | 80 | 784 | 866 | 4.38e-32 | 116 |
| Msa1050480 | MtrunA17_Chr7g0219571 | 67.500 | 80 | 20 | 1 | 1 | 80 | 800 | 873 | 8.28e-27 | 101 |
| Msa1050480 | MtrunA17_Chr4g0025961 | 65.060 | 83 | 26 | 1 | 1 | 80 | 793 | 875 | 1.39e-26 | 100 |
| Msa1050480 | MtrunA17_Chr7g0219611 | 58.750 | 80 | 22 | 1 | 1 | 80 | 780 | 848 | 2.23e-21 | 86.3 |
| Msa1050480 | MtrunA17_Chr7g0219671 | 45.652 | 92 | 37 | 4 | 1 | 79 | 798 | 889 | 3.23e-11 | 57.4 |
| Msa1050480 | MtrunA17_Chr7g0219781 | 46.739 | 92 | 36 | 4 | 1 | 79 | 801 | 892 | 7.05e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCAATGAATCAGGTTGTT+GGG | 0.380008 | 7_2:-15237354 | None:intergenic |
| TTTAGAGTTTGCGTTGCAAT+TGG | 0.422622 | 7_2:-15237329 | Msa1050480:CDS |
| CCCTCAATGAATCAGGTTGT+TGG | 0.425501 | 7_2:-15237355 | None:intergenic |
| TCAACGACCCTCAATGAATC+AGG | 0.443635 | 7_2:-15237362 | None:intergenic |
| AGTGAGGAAGATAGTGAGGT+TGG | 0.466255 | 7_2:-15237301 | Msa1050480:CDS |
| TCGCAATTTATGAGTGATGA+AGG | 0.467989 | 7_2:-15237244 | Msa1050480:CDS |
| TGTGCTATCTTTATAGCTGC+GGG | 0.503232 | 7_2:+15237198 | None:intergenic |
| CTCAATGAATCAGGTTGTTG+GGG | 0.506142 | 7_2:-15237353 | None:intergenic |
| AGAGTTTGCGTTGCAATTGG+TGG | 0.523981 | 7_2:-15237326 | Msa1050480:CDS |
| CGCAATTTATGAGTGATGAA+GGG | 0.562679 | 7_2:-15237243 | Msa1050480:CDS |
| TTGTGCTATCTTTATAGCTG+CGG | 0.578408 | 7_2:+15237197 | None:intergenic |
| GGTGAGTGAGGAAGATAGTG+AGG | 0.616318 | 7_2:-15237305 | Msa1050480:CDS |
| TCAATGAATCAGGTTGTTGG+GGG | 0.651914 | 7_2:-15237352 | Msa1050480:CDS |
| GTTGCAATTGGTGGTGAGTG+AGG | 0.655883 | 7_2:-15237317 | Msa1050480:CDS |
| AAGATAGCACAATCTCAGCG+AGG | 0.769658 | 7_2:-15237186 | Msa1050480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTCAAAGTTAACATTT+AGG | - | chr7_2:15237169-15237188 | Msa1050480:CDS | 15.0% |
| !! | TTAACATTTAGGAATTAATA+TGG | - | chr7_2:15237180-15237199 | Msa1050480:CDS | 15.0% |
| ! | TAGATCTGTCATCTCTTTTT+GGG | + | chr7_2:15236997-15237016 | None:intergenic | 30.0% |
| AAAAAGAGATGACAGATCTA+CGG | - | chr7_2:15236997-15237016 | Msa1050480:intron | 30.0% | |
| AAAAAATGTTCTTGGGTACT+TGG | + | chr7_2:15237107-15237126 | None:intergenic | 30.0% | |
| CTCAGAGAAAAAATGTTCTT+GGG | + | chr7_2:15237114-15237133 | None:intergenic | 30.0% | |
| TCTCAGAGAAAAAATGTTCT+TGG | + | chr7_2:15237115-15237134 | None:intergenic | 30.0% | |
| !! | CACGAATTTTCTAGTAAGTT+AGG | - | chr7_2:15237268-15237287 | Msa1050480:CDS | 30.0% |
| TCGATGAATCAAACATATGA+AGG | + | chr7_2:15237294-15237313 | None:intergenic | 30.0% | |
| ! | TTTAGAGTTTGCGTTGCAAT+TGG | - | chr7_2:15236938-15236957 | Msa1050480:intron | 35.0% |
| ! | GTAGATCTGTCATCTCTTTT+TGG | + | chr7_2:15236998-15237017 | None:intergenic | 35.0% |
| TCGCAATTTATGAGTGATGA+AGG | - | chr7_2:15237023-15237042 | Msa1050480:intron | 35.0% | |
| CGCAATTTATGAGTGATGAA+GGG | - | chr7_2:15237024-15237043 | Msa1050480:intron | 35.0% | |
| TTGTGCTATCTTTATAGCTG+CGG | + | chr7_2:15237073-15237092 | None:intergenic | 35.0% | |
| TGTGCTATCTTTATAGCTGC+GGG | + | chr7_2:15237072-15237091 | None:intergenic | 40.0% | |
| AGAGTTTGCGTTGCAATTGG+TGG | - | chr7_2:15236941-15236960 | Msa1050480:intron | 45.0% | |
| AGTGAGGAAGATAGTGAGGT+TGG | - | chr7_2:15236966-15236985 | Msa1050480:intron | 45.0% | |
| AAGATAGCACAATCTCAGCG+AGG | - | chr7_2:15237081-15237100 | Msa1050480:intron | 45.0% | |
| !! | GTTGCAATTGGTGGTGAGTG+AGG | - | chr7_2:15236950-15236969 | Msa1050480:intron | 50.0% |
| GGTGAGTGAGGAAGATAGTG+AGG | - | chr7_2:15236962-15236981 | Msa1050480:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 15236918 | 15237371 | 15236918 | ID=Msa1050480;Name=Msa1050480 |
| chr7_2 | mRNA | 15236918 | 15237371 | 15236918 | ID=Msa1050480-mRNA-1;Parent=Msa1050480;Name=Msa1050480-mRNA-1;_AED=0.80;_eAED=0.80;_QI=0|0|0|0.5|0|0|2|0|81 |
| chr7_2 | exon | 15237133 | 15237371 | 15237133 | ID=Msa1050480-mRNA-1:exon:3648;Parent=Msa1050480-mRNA-1 |
| chr7_2 | exon | 15236918 | 15236921 | 15236918 | ID=Msa1050480-mRNA-1:exon:3647;Parent=Msa1050480-mRNA-1 |
| chr7_2 | CDS | 15237133 | 15237371 | 15237133 | ID=Msa1050480-mRNA-1:cds;Parent=Msa1050480-mRNA-1 |
| chr7_2 | CDS | 15236918 | 15236921 | 15236918 | ID=Msa1050480-mRNA-1:cds;Parent=Msa1050480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
