AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1353670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353670 MtrunA17_Chr5g0400001 98.438 256 4 0 1 256 288 543 0.0 525
Msa1353670 MtrunA17_Chr5g0399991 93.725 255 14 1 1 255 290 542 5.20e-178 500
Msa1353670 MtrunA17_Chr4g0055281 72.549 255 63 2 1 248 279 533 1.46e-131 382
Msa1353670 MtrunA17_Chr6g0481031 58.798 233 84 2 1 221 307 539 1.12e-95 291
Msa1353670 MtrunA17_Chr4g0023601 45.887 231 110 2 1 218 214 442 2.22e-62 203
Msa1353670 MtrunA17_Chr2g0323051 43.723 231 115 2 1 218 287 515 4.37e-57 191
Msa1353670 MtrunA17_Chr8g0388651 41.379 232 125 3 1 221 281 512 6.86e-55 185
Msa1353670 MtrunA17_Chr2g0286431 37.179 234 140 3 1 230 280 510 4.79e-44 155
Msa1353670 MtrunA17_Chr7g0247631 39.130 230 125 4 1 218 466 692 2.70e-40 147
Msa1353670 MtrunA17_Chr5g0408851 33.766 231 138 5 1 221 419 644 4.55e-34 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1353670 AT4G17090.1 68.441 263 75 3 1 256 287 548 1.69e-129 378
Msa1353670 AT3G23920.1 60.515 233 78 2 3 221 309 541 1.92e-96 294
Msa1353670 AT4G15210.3 45.022 231 112 2 1 218 163 391 8.18e-61 198
Msa1353670 AT4G15210.4 45.022 231 112 2 1 218 163 391 8.18e-61 198
Msa1353670 AT4G15210.1 45.022 231 112 2 1 218 216 444 1.49e-60 199
Msa1353670 AT4G15210.2 51.351 185 88 1 1 185 216 398 3.17e-58 191
Msa1353670 AT2G32290.1 43.043 230 117 3 1 218 279 506 1.00e-56 191
Msa1353670 AT5G55700.2 40.086 232 126 3 1 219 134 365 2.57e-55 182
Msa1353670 AT5G55700.1 40.517 232 125 4 1 219 297 528 9.35e-54 182
Msa1353670 AT4G00490.1 41.597 238 124 4 1 226 296 530 2.03e-52 178
Msa1353670 AT2G45880.1 37.885 227 126 4 1 215 449 672 7.85e-38 140
Msa1353670 AT5G45300.3 34.934 229 133 6 4 221 473 696 1.80e-31 122
Msa1353670 AT5G45300.1 34.934 229 133 6 4 221 459 682 1.83e-31 122
Msa1353670 AT5G45300.2 34.934 229 133 6 4 221 457 680 1.88e-31 122
Msa1353670 AT5G18670.1 34.513 226 138 2 1 219 280 502 2.01e-31 122

Find 85 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAAGTTCCTTCAGTTTC+AGG 0.137478 tig0013921:+3606 None:intergenic
CCAATGAATTCCAGCTATTT+TGG 0.174268 tig0013921:+3148 None:intergenic
CTTTATGTTGGACACATTAA+AGG 0.225336 tig0013921:-2693 Msa1353670:CDS
AAGAAGTATATTGATTTATT+AGG 0.226783 tig0013921:-2582 Msa1353670:three_prime_UTR
ACAGTATATGAGATCTTCTT+TGG 0.254734 tig0013921:-3405 Msa1353670:intron
ACTTGTTTGGAATATGCTTT+TGG 0.267147 tig0013921:+3187 None:intergenic
TGAATTTCCTGCAAACAATT+TGG 0.270724 tig0013921:+3672 None:intergenic
TGGACACATTAAAGGAATTA+AGG 0.290030 tig0013921:-2685 Msa1353670:CDS
ACTTGGAAGTTTCCAGGAAT+TGG 0.297749 tig0013921:-3591 Msa1353670:five_prime_UTR
GGTTGCTTGAGGGTGAAAAT+TGG 0.313491 tig0013921:-2797 Msa1353670:CDS
AATCAATTTCCTGAGGATAC+TGG 0.317015 tig0013921:-3314 Msa1353670:CDS
GGAACATGGAACACAGAATA+TGG 0.319253 tig0013921:-3275 Msa1353670:CDS
CTTTCAAAATCTGATTCATA+TGG 0.321608 tig0013921:-2723 Msa1353670:CDS
CACATATTTCATCATGTTGA+TGG 0.322340 tig0013921:-2620 Msa1353670:three_prime_UTR
CATTGGAATTCTCCAATTCC+TGG 0.333977 tig0013921:+3579 None:intergenic
ATAGTTTCACTCCACTTGTT+TGG 0.337144 tig0013921:+3174 None:intergenic
ATTAAGACCAAATTGTTTGC+AGG 0.338751 tig0013921:-3679 Msa1353670:five_prime_UTR
GAAGCATCAGCTGCAGCAAT+TGG 0.348122 tig0013921:-3383 Msa1353670:CDS
ATGGAGATTTCTTCCTTGAT+TGG 0.348721 tig0013921:-3256 Msa1353670:CDS
TTTGCTAGCATTTGTGCTAT+TGG 0.361586 tig0013921:+3050 None:intergenic
GAAGGAACTTGGAAGTTTCC+AGG 0.362863 tig0013921:-3597 Msa1353670:five_prime_UTR
CAGGAAATTCAAGTGGGACT+TGG 0.366525 tig0013921:-3660 Msa1353670:five_prime_UTR
AGATATGATTCAAGTGCTTA+TGG 0.392336 tig0013921:-2876 Msa1353670:CDS
ACTAAGACTTGATGCACATT+TGG 0.400626 tig0013921:-3720 Msa1353670:five_prime_UTR
CCAAAATAGCTGGAATTCAT+TGG 0.410744 tig0013921:-3148 Msa1353670:CDS
TCCAAGTTCCTTCAGTTTCA+GGG 0.423547 tig0013921:+3607 None:intergenic
TCCACCAACATTATTGAGCT+TGG 0.439989 tig0013921:-2523 Msa1353670:three_prime_UTR
TGAAAATTGGAGGCAATTTG+TGG 0.443560 tig0013921:-2784 Msa1353670:CDS
CAGCAATTGGGAAGAAAGAA+TGG 0.447375 tig0013921:-3370 Msa1353670:CDS
CCTTCAGTTTCAGGGTAAGA+TGG 0.448441 tig0013921:+3615 None:intergenic
TTTCACATGCATGGAAATGA+AGG 0.453930 tig0013921:-3009 Msa1353670:CDS
AAACTATCTGCCAAAATAGC+TGG 0.456022 tig0013921:-3158 Msa1353670:CDS
TATGGGACTGATCTTTATGT+TGG 0.456464 tig0013921:-2705 Msa1353670:CDS
TTTGTACCTTGTCATAGCAT+TGG 0.457100 tig0013921:+3562 None:intergenic
AAGATGGCAACCAAGATTGC+GGG 0.457404 tig0013921:-2930 Msa1353670:CDS
AAGTTCACCACCCGCAATCT+TGG 0.459671 tig0013921:+2920 None:intergenic
ATTGCGGGTGGTGAACTTGC+TGG 0.464723 tig0013921:-2915 Msa1353670:CDS
ACTTGATTCACCAATCCTTC+CGG 0.472396 tig0013921:+2954 None:intergenic
TTTCAAAATCTGATTCATAT+GGG 0.479314 tig0013921:-2722 Msa1353670:CDS
TATACTAAATTTCACATGCA+TGG 0.487819 tig0013921:-3018 Msa1353670:CDS
ACCCTGAAACTGAAGGAACT+TGG 0.488235 tig0013921:-3608 Msa1353670:five_prime_UTR
TAATTGTTCACCGGAAGGAT+TGG 0.517053 tig0013921:-2964 Msa1353670:CDS
AGCAATTGGGAAGAAAGAAT+GGG 0.525174 tig0013921:-3369 Msa1353670:CDS
AAGCATCAGCTGCAGCAATT+GGG 0.535892 tig0013921:-3382 Msa1353670:CDS
ATTGTATTGACCTGAGTCAT+GGG 0.537737 tig0013921:+3334 None:intergenic
ATTGGTGAATCAAGTGAAGA+TGG 0.538317 tig0013921:-2946 Msa1353670:CDS
ATAAACAAGAGGTTGCTTGA+GGG 0.540389 tig0013921:-2807 Msa1353670:CDS
TGCTTGAGGGTGAAAATTGG+AGG 0.540642 tig0013921:-2794 Msa1353670:CDS
ACCAAGCTCAATAATGTTGG+TGG 0.545662 tig0013921:+2522 None:intergenic
GTTTGCTTGAGTACCAATCA+AGG 0.552816 tig0013921:+3243 None:intergenic
CTTATTTAAGAATAAACAAG+AGG 0.556225 tig0013921:-2818 Msa1353670:CDS
CATGCTAATTGTTCACCGGA+AGG 0.556485 tig0013921:-2969 Msa1353670:CDS
AAAAGCATATTCCAAACAAG+TGG 0.558992 tig0013921:-3185 Msa1353670:CDS
TATAACACAAGGTTTCATGA+TGG 0.559816 tig0013921:-3080 Msa1353670:CDS
CTGCAGGTTACTATAACACA+AGG 0.561651 tig0013921:-3091 Msa1353670:CDS
TTGCCATGCTAATTGTTCAC+CGG 0.569138 tig0013921:-2973 Msa1353670:CDS
GATTGTATTGACCTGAGTCA+TGG 0.571442 tig0013921:+3333 None:intergenic
CACACCAAGCTCAATAATGT+TGG 0.572891 tig0013921:+2519 None:intergenic
AATAAACAAGAGGTTGCTTG+AGG 0.573981 tig0013921:-2808 Msa1353670:CDS
AAGTGGGACTTGGTCCATGT+GGG 0.575092 tig0013921:-3650 Msa1353670:five_prime_UTR
CAAGTGGGACTTGGTCCATG+TGG 0.580992 tig0013921:-3651 Msa1353670:five_prime_UTR
GCACAAATGCTAGCAAAACA+TGG 0.581514 tig0013921:-3044 Msa1353670:CDS
GAAGATGGCAACCAAGATTG+CGG 0.585914 tig0013921:-2931 Msa1353670:CDS
CATGCAACTGAATTAACTGC+AGG 0.589620 tig0013921:-3107 Msa1353670:CDS
AAGAAAGAATGGGGAACAAG+TGG 0.590370 tig0013921:-3359 Msa1353670:CDS
ATGTTGATGGATCATGTGTA+TGG 0.592754 tig0013921:-2607 Msa1353670:three_prime_UTR
TTGTTTGCAGGAAATTCAAG+TGG 0.593690 tig0013921:-3667 Msa1353670:five_prime_UTR
ACAAGTGGACCCCATGACTC+AGG 0.595412 tig0013921:-3344 Msa1353670:CDS
CCATCTTACCCTGAAACTGA+AGG 0.596657 tig0013921:-3615 Msa1353670:five_prime_UTR
GGATATCTCAACTCCCCACA+TGG 0.597220 tig0013921:+3636 None:intergenic
AGTGGGACTTGGTCCATGTG+GGG 0.599653 tig0013921:-3649 Msa1353670:five_prime_UTR
GGTGAACAATTAGCATGGCA+AGG 0.606783 tig0013921:+2975 None:intergenic
TTTGTTGTGAGTATGTCTGA+TGG 0.609724 tig0013921:-2759 Msa1353670:CDS
ACTTGCTGGTGAGAATGCAT+TGG 0.612219 tig0013921:-2901 Msa1353670:CDS
TCAAGCAAACTTGTTGAACA+TGG 0.618498 tig0013921:-3230 Msa1353670:CDS
GTTGTGAGTATGTCTGATGG+TGG 0.636973 tig0013921:-2756 Msa1353670:CDS
TGTTTGCAGGAAATTCAAGT+GGG 0.637360 tig0013921:-3666 Msa1353670:five_prime_UTR
CTTCCGGTGAACAATTAGCA+TGG 0.643708 tig0013921:+2970 None:intergenic
ATATATCTATTCTAGACACT+AGG 0.656186 tig0013921:+2483 None:intergenic
TTGTATTGACCTGAGTCATG+GGG 0.665755 tig0013921:+3335 None:intergenic
ATGGCAACCAAGATTGCGGG+TGG 0.673251 tig0013921:-2927 Msa1353670:CDS
TCAATACAATCAATTTCCTG+AGG 0.679778 tig0013921:-3321 Msa1353670:CDS
AGAATTCCAATGCTATGACA+AGG 0.689937 tig0013921:-3568 Msa1353670:intron
GCAATTGGGAAGAAAGAATG+GGG 0.692825 tig0013921:-3368 Msa1353670:CDS
TGTCTGATGGTGGTAAACCA+AGG 0.696376 tig0013921:-2746 Msa1353670:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAGTATATTGATTTATT+AGG - tig0013921:3641-3660 Msa1353670:five_prime_UTR 15.0%
!!! AAGAAGTATATTGATTTATT+AGG - tig0013921:3641-3660 Msa1353670:five_prime_UTR 15.0%
!!! TGTTTGAAAATAAGTCATTT+TGG + tig0013921:2801-2820 None:intergenic 20.0%
!! CTTATTTAAGAATAAACAAG+AGG - tig0013921:3405-3424 Msa1353670:intron 20.0%
!! TTTCAAAATCTGATTCATAT+GGG - tig0013921:3501-3520 Msa1353670:intron 20.0%
!!! ATTAGGTTTTCTATATTTGT+TGG - tig0013921:3658-3677 Msa1353670:five_prime_UTR 20.0%
!!! TTAGGTTTTCTATATTTGTT+GGG - tig0013921:3659-3678 Msa1353670:five_prime_UTR 20.0%
!!! TGTTTGAAAATAAGTCATTT+TGG + tig0013921:2801-2820 None:intergenic 20.0%
!! CTTATTTAAGAATAAACAAG+AGG - tig0013921:3405-3424 Msa1353670:intron 20.0%
!! TTTCAAAATCTGATTCATAT+GGG - tig0013921:3501-3520 Msa1353670:intron 20.0%
!!! ATTAGGTTTTCTATATTTGT+TGG - tig0013921:3658-3677 Msa1353670:five_prime_UTR 20.0%
!!! TTAGGTTTTCTATATTTGTT+GGG - tig0013921:3659-3678 Msa1353670:five_prime_UTR 20.0%
!! GTCTAACATCAATATGATTT+TGG + tig0013921:2723-2742 None:intergenic 25.0%
! TATACTAAATTTCACATGCA+TGG - tig0013921:3205-3224 Msa1353670:CDS 25.0%
! CTTTCAAAATCTGATTCATA+TGG - tig0013921:3500-3519 Msa1353670:intron 25.0%
!! GTCTAACATCAATATGATTT+TGG + tig0013921:2723-2742 None:intergenic 25.0%
! TATACTAAATTTCACATGCA+TGG - tig0013921:3205-3224 Msa1353670:CDS 25.0%
! CTTTCAAAATCTGATTCATA+TGG - tig0013921:3500-3519 Msa1353670:intron 25.0%
ATTAAGACCAAATTGTTTGC+AGG - tig0013921:2544-2563 Msa1353670:three_prime_UTR 30.0%
TGAATTTCCTGCAAACAATT+TGG + tig0013921:2554-2573 None:intergenic 30.0%
ACAGTATATGAGATCTTCTT+TGG - tig0013921:2818-2837 Msa1353670:CDS 30.0%
TCAATACAATCAATTTCCTG+AGG - tig0013921:2902-2921 Msa1353670:CDS 30.0%
!! AGGATACTGGATTTTTCAAA+AGG - tig0013921:2922-2941 Msa1353670:CDS 30.0%
!! GGATACTGGATTTTTCAAAA+GGG - tig0013921:2923-2942 Msa1353670:CDS 30.0%
!! ACTTGTTTGGAATATGCTTT+TGG + tig0013921:3039-3058 None:intergenic 30.0%
! AAAAGCATATTCCAAACAAG+TGG - tig0013921:3038-3057 Msa1353670:CDS 30.0%
TATAACACAAGGTTTCATGA+TGG - tig0013921:3143-3162 Msa1353670:CDS 30.0%
AGATATGATTCAAGTGCTTA+TGG - tig0013921:3347-3366 Msa1353670:CDS 30.0%
CTTTATGTTGGACACATTAA+AGG - tig0013921:3530-3549 Msa1353670:intron 30.0%
TGGACACATTAAAGGAATTA+AGG - tig0013921:3538-3557 Msa1353670:intron 30.0%
CACATATTTCATCATGTTGA+TGG - tig0013921:3603-3622 Msa1353670:five_prime_UTR 30.0%
ATTAAGACCAAATTGTTTGC+AGG - tig0013921:2544-2563 Msa1353670:three_prime_UTR 30.0%
TGAATTTCCTGCAAACAATT+TGG + tig0013921:2554-2573 None:intergenic 30.0%
ACAGTATATGAGATCTTCTT+TGG - tig0013921:2818-2837 Msa1353670:CDS 30.0%
TCAATACAATCAATTTCCTG+AGG - tig0013921:2902-2921 Msa1353670:CDS 30.0%
!! AGGATACTGGATTTTTCAAA+AGG - tig0013921:2922-2941 Msa1353670:CDS 30.0%
!! GGATACTGGATTTTTCAAAA+GGG - tig0013921:2923-2942 Msa1353670:CDS 30.0%
!! ACTTGTTTGGAATATGCTTT+TGG + tig0013921:3039-3058 None:intergenic 30.0%
! AAAAGCATATTCCAAACAAG+TGG - tig0013921:3038-3057 Msa1353670:CDS 30.0%
TATAACACAAGGTTTCATGA+TGG - tig0013921:3143-3162 Msa1353670:CDS 30.0%
AGATATGATTCAAGTGCTTA+TGG - tig0013921:3347-3366 Msa1353670:CDS 30.0%
CTTTATGTTGGACACATTAA+AGG - tig0013921:3530-3549 Msa1353670:intron 30.0%
TGGACACATTAAAGGAATTA+AGG - tig0013921:3538-3557 Msa1353670:intron 30.0%
CACATATTTCATCATGTTGA+TGG - tig0013921:3603-3622 Msa1353670:five_prime_UTR 30.0%
ACTAAGACTTGATGCACATT+TGG - tig0013921:2503-2522 Msa1353670:three_prime_UTR 35.0%
TTGTTTGCAGGAAATTCAAG+TGG - tig0013921:2556-2575 Msa1353670:three_prime_UTR 35.0%
TGTTTGCAGGAAATTCAAGT+GGG - tig0013921:2557-2576 Msa1353670:three_prime_UTR 35.0%
AGAATTCCAATGCTATGACA+AGG - tig0013921:2655-2674 Msa1353670:CDS 35.0%
TTTGTACCTTGTCATAGCAT+TGG + tig0013921:2664-2683 None:intergenic 35.0%
AGCAATTGGGAAGAAAGAAT+GGG - tig0013921:2854-2873 Msa1353670:CDS 35.0%
! ATTGTATTGACCTGAGTCAT+GGG + tig0013921:2892-2911 None:intergenic 35.0%
AATCAATTTCCTGAGGATAC+TGG - tig0013921:2909-2928 Msa1353670:CDS 35.0%
TTGAAAAATCCAGTATCCTC+AGG + tig0013921:2921-2940 None:intergenic 35.0%
!! ACTGGATTTTTCAAAAGGGA+TGG - tig0013921:2927-2946 Msa1353670:CDS 35.0%
! TTTTCAAAAGGGATGGAACA+TGG - tig0013921:2934-2953 Msa1353670:CDS 35.0%
ATGGAGATTTCTTCCTTGAT+TGG - tig0013921:2967-2986 Msa1353670:CDS 35.0%
!! TCAAGCAAACTTGTTGAACA+TGG - tig0013921:2993-3012 Msa1353670:CDS 35.0%
!! ATATGCTTTTGGCTGATACA+AGG + tig0013921:3028-3047 None:intergenic 35.0%
ATAGTTTCACTCCACTTGTT+TGG + tig0013921:3052-3071 None:intergenic 35.0%
AAACTATCTGCCAAAATAGC+TGG - tig0013921:3065-3084 Msa1353670:CDS 35.0%
! CCAATGAATTCCAGCTATTT+TGG + tig0013921:3078-3097 None:intergenic 35.0%
CCAAAATAGCTGGAATTCAT+TGG - tig0013921:3075-3094 Msa1353670:CDS 35.0%
TTTGCTAGCATTTGTGCTAT+TGG + tig0013921:3176-3195 None:intergenic 35.0%
TTTCACATGCATGGAAATGA+AGG - tig0013921:3214-3233 Msa1353670:CDS 35.0%
ATTGGTGAATCAAGTGAAGA+TGG - tig0013921:3277-3296 Msa1353670:CDS 35.0%
AATAAACAAGAGGTTGCTTG+AGG - tig0013921:3415-3434 Msa1353670:intron 35.0%
ATAAACAAGAGGTTGCTTGA+GGG - tig0013921:3416-3435 Msa1353670:intron 35.0%
TGAAAATTGGAGGCAATTTG+TGG - tig0013921:3439-3458 Msa1353670:intron 35.0%
! TTTGTTGTGAGTATGTCTGA+TGG - tig0013921:3464-3483 Msa1353670:intron 35.0%
!! GAATCAGATTTTGAAAGCCT+TGG + tig0013921:3497-3516 None:intergenic 35.0%
TATGGGACTGATCTTTATGT+TGG - tig0013921:3518-3537 Msa1353670:intron 35.0%
! ATGTTGATGGATCATGTGTA+TGG - tig0013921:3616-3635 Msa1353670:five_prime_UTR 35.0%
ACTAAGACTTGATGCACATT+TGG - tig0013921:2503-2522 Msa1353670:three_prime_UTR 35.0%
TTGTTTGCAGGAAATTCAAG+TGG - tig0013921:2556-2575 Msa1353670:three_prime_UTR 35.0%
TGTTTGCAGGAAATTCAAGT+GGG - tig0013921:2557-2576 Msa1353670:three_prime_UTR 35.0%
AGAATTCCAATGCTATGACA+AGG - tig0013921:2655-2674 Msa1353670:CDS 35.0%
TTTGTACCTTGTCATAGCAT+TGG + tig0013921:2664-2683 None:intergenic 35.0%
AGCAATTGGGAAGAAAGAAT+GGG - tig0013921:2854-2873 Msa1353670:CDS 35.0%
! ATTGTATTGACCTGAGTCAT+GGG + tig0013921:2892-2911 None:intergenic 35.0%
AATCAATTTCCTGAGGATAC+TGG - tig0013921:2909-2928 Msa1353670:CDS 35.0%
TTGAAAAATCCAGTATCCTC+AGG + tig0013921:2921-2940 None:intergenic 35.0%
!! ACTGGATTTTTCAAAAGGGA+TGG - tig0013921:2927-2946 Msa1353670:CDS 35.0%
! TTTTCAAAAGGGATGGAACA+TGG - tig0013921:2934-2953 Msa1353670:CDS 35.0%
ATGGAGATTTCTTCCTTGAT+TGG - tig0013921:2967-2986 Msa1353670:CDS 35.0%
!! TCAAGCAAACTTGTTGAACA+TGG - tig0013921:2993-3012 Msa1353670:CDS 35.0%
!! ATATGCTTTTGGCTGATACA+AGG + tig0013921:3028-3047 None:intergenic 35.0%
ATAGTTTCACTCCACTTGTT+TGG + tig0013921:3052-3071 None:intergenic 35.0%
AAACTATCTGCCAAAATAGC+TGG - tig0013921:3065-3084 Msa1353670:CDS 35.0%
! CCAATGAATTCCAGCTATTT+TGG + tig0013921:3078-3097 None:intergenic 35.0%
CCAAAATAGCTGGAATTCAT+TGG - tig0013921:3075-3094 Msa1353670:CDS 35.0%
TTTGCTAGCATTTGTGCTAT+TGG + tig0013921:3176-3195 None:intergenic 35.0%
TTTCACATGCATGGAAATGA+AGG - tig0013921:3214-3233 Msa1353670:CDS 35.0%
ATTGGTGAATCAAGTGAAGA+TGG - tig0013921:3277-3296 Msa1353670:CDS 35.0%
AATAAACAAGAGGTTGCTTG+AGG - tig0013921:3415-3434 Msa1353670:intron 35.0%
ATAAACAAGAGGTTGCTTGA+GGG - tig0013921:3416-3435 Msa1353670:intron 35.0%
TGAAAATTGGAGGCAATTTG+TGG - tig0013921:3439-3458 Msa1353670:intron 35.0%
! TTTGTTGTGAGTATGTCTGA+TGG - tig0013921:3464-3483 Msa1353670:intron 35.0%
!! GAATCAGATTTTGAAAGCCT+TGG + tig0013921:3497-3516 None:intergenic 35.0%
TATGGGACTGATCTTTATGT+TGG - tig0013921:3518-3537 Msa1353670:intron 35.0%
! ATGTTGATGGATCATGTGTA+TGG - tig0013921:3616-3635 Msa1353670:five_prime_UTR 35.0%
TCCAAGTTCCTTCAGTTTCA+GGG + tig0013921:2619-2638 None:intergenic 40.0%
TTCCAAGTTCCTTCAGTTTC+AGG + tig0013921:2620-2639 None:intergenic 40.0%
ACTTGGAAGTTTCCAGGAAT+TGG - tig0013921:2632-2651 Msa1353670:exon 40.0%
CATTGGAATTCTCCAATTCC+TGG + tig0013921:2647-2666 None:intergenic 40.0%
CAGCAATTGGGAAGAAAGAA+TGG - tig0013921:2853-2872 Msa1353670:CDS 40.0%
GCAATTGGGAAGAAAGAATG+GGG - tig0013921:2855-2874 Msa1353670:CDS 40.0%
AAGAAAGAATGGGGAACAAG+TGG - tig0013921:2864-2883 Msa1353670:CDS 40.0%
! TTGTATTGACCTGAGTCATG+GGG + tig0013921:2891-2910 None:intergenic 40.0%
! GATTGTATTGACCTGAGTCA+TGG + tig0013921:2893-2912 None:intergenic 40.0%
GGAACATGGAACACAGAATA+TGG - tig0013921:2948-2967 Msa1353670:CDS 40.0%
GTTTGCTTGAGTACCAATCA+AGG + tig0013921:2983-3002 None:intergenic 40.0%
CATGCAACTGAATTAACTGC+AGG - tig0013921:3116-3135 Msa1353670:CDS 40.0%
CTGCAGGTTACTATAACACA+AGG - tig0013921:3132-3151 Msa1353670:CDS 40.0%
GCACAAATGCTAGCAAAACA+TGG - tig0013921:3179-3198 Msa1353670:CDS 40.0%
TTGCCATGCTAATTGTTCAC+CGG - tig0013921:3250-3269 Msa1353670:CDS 40.0%
TAATTGTTCACCGGAAGGAT+TGG - tig0013921:3259-3278 Msa1353670:CDS 40.0%
ACTTGATTCACCAATCCTTC+CGG + tig0013921:3272-3291 None:intergenic 40.0%
!! GGTCAAGTTTTGTCAACAAG+TGG - tig0013921:3368-3387 Msa1353670:CDS 40.0%
TCCACCAACATTATTGAGCT+TGG - tig0013921:3700-3719 Msa1353670:five_prime_UTR 40.0%
ACCAAGCTCAATAATGTTGG+TGG + tig0013921:3704-3723 None:intergenic 40.0%
CACACCAAGCTCAATAATGT+TGG + tig0013921:3707-3726 None:intergenic 40.0%
TCCAAGTTCCTTCAGTTTCA+GGG + tig0013921:2619-2638 None:intergenic 40.0%
TTCCAAGTTCCTTCAGTTTC+AGG + tig0013921:2620-2639 None:intergenic 40.0%
ACTTGGAAGTTTCCAGGAAT+TGG - tig0013921:2632-2651 Msa1353670:exon 40.0%
CATTGGAATTCTCCAATTCC+TGG + tig0013921:2647-2666 None:intergenic 40.0%
CAGCAATTGGGAAGAAAGAA+TGG - tig0013921:2853-2872 Msa1353670:CDS 40.0%
GCAATTGGGAAGAAAGAATG+GGG - tig0013921:2855-2874 Msa1353670:CDS 40.0%
AAGAAAGAATGGGGAACAAG+TGG - tig0013921:2864-2883 Msa1353670:CDS 40.0%
! TTGTATTGACCTGAGTCATG+GGG + tig0013921:2891-2910 None:intergenic 40.0%
! GATTGTATTGACCTGAGTCA+TGG + tig0013921:2893-2912 None:intergenic 40.0%
GGAACATGGAACACAGAATA+TGG - tig0013921:2948-2967 Msa1353670:CDS 40.0%
GTTTGCTTGAGTACCAATCA+AGG + tig0013921:2983-3002 None:intergenic 40.0%
CATGCAACTGAATTAACTGC+AGG - tig0013921:3116-3135 Msa1353670:CDS 40.0%
CTGCAGGTTACTATAACACA+AGG - tig0013921:3132-3151 Msa1353670:CDS 40.0%
GCACAAATGCTAGCAAAACA+TGG - tig0013921:3179-3198 Msa1353670:CDS 40.0%
TTGCCATGCTAATTGTTCAC+CGG - tig0013921:3250-3269 Msa1353670:CDS 40.0%
TAATTGTTCACCGGAAGGAT+TGG - tig0013921:3259-3278 Msa1353670:CDS 40.0%
ACTTGATTCACCAATCCTTC+CGG + tig0013921:3272-3291 None:intergenic 40.0%
!! GGTCAAGTTTTGTCAACAAG+TGG - tig0013921:3368-3387 Msa1353670:CDS 40.0%
TCCACCAACATTATTGAGCT+TGG - tig0013921:3700-3719 Msa1353670:five_prime_UTR 40.0%
ACCAAGCTCAATAATGTTGG+TGG + tig0013921:3704-3723 None:intergenic 40.0%
CACACCAAGCTCAATAATGT+TGG + tig0013921:3707-3726 None:intergenic 40.0%
CAGGAAATTCAAGTGGGACT+TGG - tig0013921:2563-2582 Msa1353670:three_prime_UTR 45.0%
CCTTCAGTTTCAGGGTAAGA+TGG + tig0013921:2611-2630 None:intergenic 45.0%
CCATCTTACCCTGAAACTGA+AGG - tig0013921:2608-2627 Msa1353670:three_prime_UTR 45.0%
ACCCTGAAACTGAAGGAACT+TGG - tig0013921:2615-2634 Msa1353670:three_prime_UTR 45.0%
GAAGGAACTTGGAAGTTTCC+AGG - tig0013921:2626-2645 Msa1353670:exon 45.0%
AAGCATCAGCTGCAGCAATT+GGG - tig0013921:2841-2860 Msa1353670:CDS 45.0%
GGTGAACAATTAGCATGGCA+AGG + tig0013921:3251-3270 None:intergenic 45.0%
CTTCCGGTGAACAATTAGCA+TGG + tig0013921:3256-3275 None:intergenic 45.0%
CATGCTAATTGTTCACCGGA+AGG - tig0013921:3254-3273 Msa1353670:CDS 45.0%
GAAGATGGCAACCAAGATTG+CGG - tig0013921:3292-3311 Msa1353670:CDS 45.0%
AAGATGGCAACCAAGATTGC+GGG - tig0013921:3293-3312 Msa1353670:CDS 45.0%
! ACTTGCTGGTGAGAATGCAT+TGG - tig0013921:3322-3341 Msa1353670:CDS 45.0%
GGTTGCTTGAGGGTGAAAAT+TGG - tig0013921:3426-3445 Msa1353670:intron 45.0%
TGCTTGAGGGTGAAAATTGG+AGG - tig0013921:3429-3448 Msa1353670:intron 45.0%
GTTGTGAGTATGTCTGATGG+TGG - tig0013921:3467-3486 Msa1353670:intron 45.0%
!! TGTCTGATGGTGGTAAACCA+AGG - tig0013921:3477-3496 Msa1353670:intron 45.0%
CAGGAAATTCAAGTGGGACT+TGG - tig0013921:2563-2582 Msa1353670:three_prime_UTR 45.0%
CCTTCAGTTTCAGGGTAAGA+TGG + tig0013921:2611-2630 None:intergenic 45.0%
CCATCTTACCCTGAAACTGA+AGG - tig0013921:2608-2627 Msa1353670:three_prime_UTR 45.0%
ACCCTGAAACTGAAGGAACT+TGG - tig0013921:2615-2634 Msa1353670:three_prime_UTR 45.0%
GAAGGAACTTGGAAGTTTCC+AGG - tig0013921:2626-2645 Msa1353670:exon 45.0%
AAGCATCAGCTGCAGCAATT+GGG - tig0013921:2841-2860 Msa1353670:CDS 45.0%
GGTGAACAATTAGCATGGCA+AGG + tig0013921:3251-3270 None:intergenic 45.0%
CTTCCGGTGAACAATTAGCA+TGG + tig0013921:3256-3275 None:intergenic 45.0%
CATGCTAATTGTTCACCGGA+AGG - tig0013921:3254-3273 Msa1353670:CDS 45.0%
GAAGATGGCAACCAAGATTG+CGG - tig0013921:3292-3311 Msa1353670:CDS 45.0%
AAGATGGCAACCAAGATTGC+GGG - tig0013921:3293-3312 Msa1353670:CDS 45.0%
! ACTTGCTGGTGAGAATGCAT+TGG - tig0013921:3322-3341 Msa1353670:CDS 45.0%
GGTTGCTTGAGGGTGAAAAT+TGG - tig0013921:3426-3445 Msa1353670:intron 45.0%
TGCTTGAGGGTGAAAATTGG+AGG - tig0013921:3429-3448 Msa1353670:intron 45.0%
GTTGTGAGTATGTCTGATGG+TGG - tig0013921:3467-3486 Msa1353670:intron 45.0%
!! TGTCTGATGGTGGTAAACCA+AGG - tig0013921:3477-3496 Msa1353670:intron 45.0%
! AAGTGGGACTTGGTCCATGT+GGG - tig0013921:2573-2592 Msa1353670:three_prime_UTR 50.0%
GGATATCTCAACTCCCCACA+TGG + tig0013921:2590-2609 None:intergenic 50.0%
! GAAGCATCAGCTGCAGCAAT+TGG - tig0013921:2840-2859 Msa1353670:CDS 50.0%
AAGTTCACCACCCGCAATCT+TGG + tig0013921:3306-3325 None:intergenic 50.0%
! AAGTGGGACTTGGTCCATGT+GGG - tig0013921:2573-2592 Msa1353670:three_prime_UTR 50.0%
GGATATCTCAACTCCCCACA+TGG + tig0013921:2590-2609 None:intergenic 50.0%
! GAAGCATCAGCTGCAGCAAT+TGG - tig0013921:2840-2859 Msa1353670:CDS 50.0%
AAGTTCACCACCCGCAATCT+TGG + tig0013921:3306-3325 None:intergenic 50.0%
! CAAGTGGGACTTGGTCCATG+TGG - tig0013921:2572-2591 Msa1353670:three_prime_UTR 55.0%
! AGTGGGACTTGGTCCATGTG+GGG - tig0013921:2574-2593 Msa1353670:three_prime_UTR 55.0%
ACAAGTGGACCCCATGACTC+AGG - tig0013921:2879-2898 Msa1353670:CDS 55.0%
ATGGCAACCAAGATTGCGGG+TGG - tig0013921:3296-3315 Msa1353670:CDS 55.0%
ATTGCGGGTGGTGAACTTGC+TGG - tig0013921:3308-3327 Msa1353670:CDS 55.0%
! CAAGTGGGACTTGGTCCATG+TGG - tig0013921:2572-2591 Msa1353670:three_prime_UTR 55.0%
! AGTGGGACTTGGTCCATGTG+GGG - tig0013921:2574-2593 Msa1353670:three_prime_UTR 55.0%
ACAAGTGGACCCCATGACTC+AGG - tig0013921:2879-2898 Msa1353670:CDS 55.0%
ATGGCAACCAAGATTGCGGG+TGG - tig0013921:3296-3315 Msa1353670:CDS 55.0%
ATTGCGGGTGGTGAACTTGC+TGG - tig0013921:3308-3327 Msa1353670:CDS 55.0%
Chromosome Type Strat End Strand Name
tig0013921 gene 2487 3758 2487 ID=Msa1353670;Name=Msa1353670
tig0013921 mRNA 2487 3758 2487 ID=Msa1353670-mRNA-1;Parent=Msa1353670;Name=Msa1353670-mRNA-1;_AED=0.01;_eAED=0.01;_QI=190|0|0.5|1|0|1|2|157|258
tig0013921 exon 2487 3420 2487 ID=Msa1353670-mRNA-1:exon:8780;Parent=Msa1353670-mRNA-1
tig0013921 exon 3569 3758 3569 ID=Msa1353670-mRNA-1:exon:8779;Parent=Msa1353670-mRNA-1
tig0013921 five_prime_UTR 3569 3758 3569 ID=Msa1353670-mRNA-1:five_prime_utr;Parent=Msa1353670-mRNA-1
tig0013921 CDS 2644 3420 2644 ID=Msa1353670-mRNA-1:cds;Parent=Msa1353670-mRNA-1
tig0013921 three_prime_UTR 2487 2643 2487 ID=Msa1353670-mRNA-1:three_prime_utr;Parent=Msa1353670-mRNA-1
Gene Sequence

>Msa1353670

ATGAGATCTTCTTTGGAAGCATCAGCTGCAGCAATTGGGAAGAAAGAATGGGGAACAAGTGGACCCCATGACTCAGGTCAATACAATCAATTTCCTGAGGATACTGGATTTTTCAAAAGGGATGGAACATGGAACACAGAATATGGAGATTTCTTCCTTGATTGGTACTCAAGCAAACTTGTTGAACATGGTGAGAAAATCCTTGTATCAGCCAAAAGCATATTCCAAACAAGTGGAGTGAAACTATCTGCCAAAATAGCTGGAATTCATTGGCATTACAATGCAAGATCACATGCAACTGAATTAACTGCAGGTTACTATAACACAAGGTTTCATGATGGTTATATACCAATAGCACAAATGCTAGCAAAACATGGAGTTATACTAAATTTCACATGCATGGAAATGAAGGATAATGAACAACCTTGCCATGCTAATTGTTCACCGGAAGGATTGGTGAATCAAGTGAAGATGGCAACCAAGATTGCGGGTGGTGAACTTGCTGGTGAGAATGCATTGGAGAGATATGATTCAAGTGCTTATGGTCAAGTTTTGTCAACAAGTGGATTGAGTGCTTTTACTTATTTAAGAATAAACAAGAGGTTGCTTGAGGGTGAAAATTGGAGGCAATTTGTGGACTTTGTTGTGAGTATGTCTGATGGTGGTAAACCAAGGCTTTCAAAATCTGATTCATATGGGACTGATCTTTATGTTGGACACATTAAAGGAATTAAGGAGAGTGAAGTTATAATAGAGATTGCTCTTTGTGAACATTGA
Protein sequence

>Msa1353670

MRSSLEASAAAIGKKEWGTSGPHDSGQYNQFPEDTGFFKRDGTWNTEYGDFFLDWYSSKLVEHGEKILVSAKSIFQTSGVKLSAKIAGIHWHYNARSHATELTAGYYNTRFHDGYIPIAQMLAKHGVILNFTCMEMKDNEQPCHANCSPEGLVNQVKMATKIAGGELAGENALERYDSSAYGQVLSTSGLSAFTYLRINKRLLEGENWRQFVDFVVSMSDGGKPRLSKSDSYGTDLYVGHIKGIKESEVIIEIALCEH*