Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0579350 | XP_003607784.1 | 90.123 | 81 | 8 | 0 | 1 | 81 | 1 | 81 | 1.39e-35 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0579350 | G7JHG4 | 90.123 | 81 | 8 | 0 | 1 | 81 | 1 | 81 | 6.64e-36 | 125 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0197180 | Msa0579350 | 0.803600 | 3.226584e-49 | -8.615850e-47 |
| Msa0377010 | Msa0579350 | 0.802657 | 5.056049e-49 | -8.615850e-47 |
| Msa0379380 | Msa0579350 | 0.800957 | 1.129445e-48 | -8.615850e-47 |
| Msa0426810 | Msa0579350 | 0.809268 | 2.058052e-50 | -8.615850e-47 |
| Msa0435000 | Msa0579350 | 0.828756 | 7.533802e-55 | -8.615850e-47 |
| Msa0453280 | Msa0579350 | 0.806205 | 9.209633e-50 | -8.615850e-47 |
| Msa0475240 | Msa0579350 | 0.811519 | 6.721367e-51 | -8.615850e-47 |
| Msa0479040 | Msa0579350 | 0.819248 | 1.283218e-52 | -8.615850e-47 |
| Msa0479090 | Msa0579350 | 0.830324 | 3.131239e-55 | -8.615850e-47 |
| Msa0479940 | Msa0579350 | 0.807699 | 4.448243e-50 | -8.615850e-47 |
| Msa0238940 | Msa0579350 | 0.802344 | 5.866316e-49 | -8.615850e-47 |
| Msa0266210 | Msa0579350 | 0.822432 | 2.375810e-53 | -8.615850e-47 |
| Msa0287670 | Msa0579350 | 0.827227 | 1.757620e-54 | -8.615850e-47 |
| Msa0316750 | Msa0579350 | 0.800321 | 1.522616e-48 | -8.615850e-47 |
| Msa0361010 | Msa0579350 | 0.805515 | 1.286222e-49 | -8.615850e-47 |
| Msa0362060 | Msa0579350 | 0.841777 | 3.869027e-58 | -8.615850e-47 |
| Msa0579340 | Msa0579350 | 0.803961 | 2.715400e-49 | -8.615850e-47 |
| Msa0579350 | Msa0579360 | 0.831084 | 2.039347e-55 | -8.615850e-47 |
| Msa0579350 | Msa0579370 | 0.853389 | 2.468746e-61 | -8.615850e-47 |
| Msa0579350 | Msa0598560 | 0.811425 | 7.047117e-51 | -8.615850e-47 |
| Msa0579350 | Msa0609970 | 0.818379 | 2.021324e-52 | -8.615850e-47 |
| Msa0579350 | Msa0614990 | 0.876641 | 1.188231e-68 | -8.615850e-47 |
| Msa0579350 | Msa0644770 | -0.802130 | 6.490672e-49 | -8.615850e-47 |
| Msa0579350 | Msa0650620 | 0.849766 | 2.618516e-60 | -8.615850e-47 |
| Msa0579350 | Msa0650640 | 0.835704 | 1.437417e-56 | -8.615850e-47 |
| Msa0579350 | Msa0650730 | 0.892093 | 2.180034e-74 | -8.615850e-47 |
| Msa0579350 | Msa0709670 | 0.807645 | 4.567960e-50 | -8.615850e-47 |
| Msa0579350 | Msa0713210 | 0.822843 | 1.906735e-53 | -8.615850e-47 |
| Msa0579350 | Msa0713220 | 0.815502 | 8.945455e-52 | -8.615850e-47 |
| Msa0579350 | Msa0713230 | 0.854804 | 9.641790e-62 | -8.615850e-47 |
| Msa0579350 | Msa0755080 | 0.876554 | 1.273206e-68 | -8.615850e-47 |
| Msa0579350 | Msa0796950 | 0.852751 | 3.758943e-61 | -8.615850e-47 |
| Msa0579350 | Msa0797000 | 0.831082 | 2.042173e-55 | -8.615850e-47 |
| Msa0579350 | Msa0834450 | 0.873700 | 1.199780e-67 | -8.615850e-47 |
| Msa0579350 | Msa0834460 | 0.811404 | 7.119886e-51 | -8.615850e-47 |
| Msa0579350 | Msa0898860 | 0.813142 | 2.973413e-51 | -8.615850e-47 |
| Msa0579350 | Msa0928030 | 0.831460 | 1.648530e-55 | -8.615850e-47 |
| Msa0579350 | Msa1075590 | 0.814508 | 1.487009e-51 | -8.615850e-47 |
| Msa0579350 | Msa1137090 | 0.818245 | 2.167141e-52 | -8.615850e-47 |
| Msa0579350 | Msa1182640 | 0.821732 | 3.453142e-53 | -8.615850e-47 |
| Msa0579350 | Msa1350690 | 0.825125 | 5.557751e-54 | -8.615850e-47 |
| Msa0579350 | Msa1353670 | 0.812481 | 4.148076e-51 | -8.615850e-47 |
| Msa0579350 | Msa1356180 | 0.817884 | 2.615941e-52 | -8.615850e-47 |
| Msa0579350 | Msa1360280 | 0.812229 | 4.709125e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0579350 | MtrunA17_Chr4g0042971 | 46.250 | 80 | 37 | 2 | 4 | 81 | 81 | 156 | 4.16e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 19 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTGGACCTAACGATATTT+TGG | 0.245207 | 4_1:-84005180 | Msa0579350:CDS |
| AAAACCAAAGGTGCGGTTAA+TGG | 0.283737 | 4_1:-84004993 | Msa0579350:CDS |
| TTTGCCAATAGGATCTGGTT+TGG | 0.364938 | 4_1:+84005013 | None:intergenic |
| TTGCCAATAGGATCTGGTTT+GGG | 0.376061 | 4_1:+84005014 | None:intergenic |
| AGCACCATTAACCGCACCTT+TGG | 0.426753 | 4_1:+84004989 | None:intergenic |
| GCTTCAGCACAGAAAACTAT+AGG | 0.438359 | 4_1:-84005137 | Msa0579350:CDS |
| GCACTTGCCAAAATATCGTT+AGG | 0.455143 | 4_1:+84005173 | None:intergenic |
| AAACCCAAACCAGATCCTAT+TGG | 0.473112 | 4_1:-84005017 | Msa0579350:CDS |
| TAAACAACAAAATCGATGGC+TGG | 0.494913 | 4_1:-84005197 | None:intergenic |
| ACTATAGGAAATGCTGCAGC+TGG | 0.550456 | 4_1:-84005122 | Msa0579350:CDS |
| GGTGCTACAAAGTCTGTTGG+AGG | 0.564564 | 4_1:-84004972 | Msa0579350:CDS |
| GCTGGTGTTCATAAGACTGT+AGG | 0.573068 | 4_1:-84005071 | Msa0579350:CDS |
| AATGGTGCTACAAAGTCTGT+TGG | 0.575777 | 4_1:-84004975 | Msa0579350:CDS |
| ACTGTGGGAAATGCTGCAGC+TGG | 0.580044 | 4_1:-84005089 | Msa0579350:CDS |
| GATCCTATTGGCAAAACCAA+AGG | 0.604323 | 4_1:-84005005 | Msa0579350:CDS |
| AGCTGGTGCCCAGAAAACTG+TGG | 0.654418 | 4_1:-84005105 | Msa0579350:CDS |
| TGTAGGAGACTATGCTACCA+TGG | 0.655972 | 4_1:-84005054 | Msa0579350:CDS |
| TATTGGCAAAACCAAAGGTG+CGG | 0.681681 | 4_1:-84005000 | Msa0579350:CDS |
| GCTGGTGCCCAGAAAACTGT+GGG | 0.683027 | 4_1:-84005104 | Msa0579350:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTTACATAATCAGCAGCCA+TGG | + | chr4_1:84005107-84005126 | None:intergenic | 35.0% |
| GCACTTGCCAAAATATCGTT+AGG | + | chr4_1:84004971-84004990 | None:intergenic | 40.0% | |
| !!! | CGATATTTTGGCAAGTGCAA+AGG | - | chr4_1:84004973-84004992 | Msa0579350:CDS | 40.0% |
| GCTTCAGCACAGAAAACTAT+AGG | - | chr4_1:84005004-84005023 | Msa0579350:CDS | 40.0% | |
| ! | AGCATTTCCCACAGTTTTCT+GGG | + | chr4_1:84005047-84005066 | None:intergenic | 40.0% |
| AAACCCAAACCAGATCCTAT+TGG | - | chr4_1:84005124-84005143 | Msa0579350:CDS | 40.0% | |
| ! | TTGCCAATAGGATCTGGTTT+GGG | + | chr4_1:84005130-84005149 | None:intergenic | 40.0% |
| TTTGCCAATAGGATCTGGTT+TGG | + | chr4_1:84005131-84005150 | None:intergenic | 40.0% | |
| !!! | TTGGTTTTGCCAATAGGATC+TGG | + | chr4_1:84005136-84005155 | None:intergenic | 40.0% |
| GATCCTATTGGCAAAACCAA+AGG | - | chr4_1:84005136-84005155 | Msa0579350:CDS | 40.0% | |
| TATTGGCAAAACCAAAGGTG+CGG | - | chr4_1:84005141-84005160 | Msa0579350:CDS | 40.0% | |
| ! | AAAACCAAAGGTGCGGTTAA+TGG | - | chr4_1:84005148-84005167 | Msa0579350:CDS | 40.0% |
| !! | AATGGTGCTACAAAGTCTGT+TGG | - | chr4_1:84005166-84005185 | Msa0579350:CDS | 40.0% |
| ! | GGCTGGACCTAACGATATTT+TGG | - | chr4_1:84004961-84004980 | Msa0579350:CDS | 45.0% |
| ACTATAGGAAATGCTGCAGC+TGG | - | chr4_1:84005019-84005038 | Msa0579350:CDS | 45.0% | |
| ! | CAGCATTTCCCACAGTTTTC+TGG | + | chr4_1:84005048-84005067 | None:intergenic | 45.0% |
| GCTGGTGTTCATAAGACTGT+AGG | - | chr4_1:84005070-84005089 | Msa0579350:CDS | 45.0% | |
| TGTAGGAGACTATGCTACCA+TGG | - | chr4_1:84005087-84005106 | Msa0579350:CDS | 45.0% | |
| !!! | GCACCTTTGGTTTTGCCAAT+AGG | + | chr4_1:84005142-84005161 | None:intergenic | 45.0% |
| AGCACCATTAACCGCACCTT+TGG | + | chr4_1:84005155-84005174 | None:intergenic | 50.0% | |
| ! | GGTGCTACAAAGTCTGTTGG+AGG | - | chr4_1:84005169-84005188 | Msa0579350:CDS | 50.0% |
| AGCTGGTGCCCAGAAAACTG+TGG | - | chr4_1:84005036-84005055 | Msa0579350:CDS | 55.0% | |
| GCTGGTGCCCAGAAAACTGT+GGG | - | chr4_1:84005037-84005056 | Msa0579350:CDS | 55.0% | |
| ACTGTGGGAAATGCTGCAGC+TGG | - | chr4_1:84005052-84005071 | Msa0579350:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_1 | gene | 84004959 | 84005204 | 84004959 | ID=Msa0579350;Name=Msa0579350 |
| chr4_1 | mRNA | 84004959 | 84005204 | 84004959 | ID=Msa0579350-mRNA-1;Parent=Msa0579350;Name=Msa0579350-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|81 |
| chr4_1 | exon | 84004959 | 84005204 | 84004959 | ID=Msa0579350-mRNA-1:exon:30786;Parent=Msa0579350-mRNA-1 |
| chr4_1 | CDS | 84004959 | 84005204 | 84004959 | ID=Msa0579350-mRNA-1:cds;Parent=Msa0579350-mRNA-1 |
| Gene Sequence |
| Protein sequence |
