Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386690 | XP_003594274.1 | 93.056 | 144 | 6 | 2 | 1 | 143 | 1 | 141 | 3.26e-92 | 273 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386690 | G7IPB5 | 93.056 | 144 | 6 | 2 | 1 | 143 | 1 | 141 | 1.56e-92 | 273 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa1386690 | 0.812140 | 4.925166e-51 | -8.615850e-47 |
| Msa0072370 | Msa1386690 | 0.814701 | 1.347090e-51 | -8.615850e-47 |
| Msa0110970 | Msa1386690 | 0.816237 | 6.131961e-52 | -8.615850e-47 |
| Msa0124280 | Msa1386690 | 0.808224 | 3.440796e-50 | -8.615850e-47 |
| Msa0157290 | Msa1386690 | 0.812543 | 4.020463e-51 | -8.615850e-47 |
| Msa0233730 | Msa1386690 | 0.804853 | 1.769208e-49 | -8.615850e-47 |
| Msa1271990 | Msa1386690 | 0.802114 | 6.540966e-49 | -8.615850e-47 |
| Msa0345330 | Msa1386690 | 0.814176 | 1.760656e-51 | -8.615850e-47 |
| Msa0345410 | Msa1386690 | 0.810948 | 8.943840e-51 | -8.615850e-47 |
| Msa1151910 | Msa1386690 | 0.803340 | 3.653035e-49 | -8.615850e-47 |
| Msa0880140 | Msa1386690 | 0.809857 | 1.537491e-50 | -8.615850e-47 |
| Msa0895790 | Msa1386690 | 0.800768 | 1.234532e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1386690 | MtrunA17_Chr2g0290261 | 93.056 | 144 | 6 | 2 | 1 | 143 | 1 | 141 | 3.00e-96 | 273 |
| Msa1386690 | MtrunA17_Chr4g0076121 | 49.686 | 159 | 54 | 7 | 1 | 143 | 1 | 149 | 1.60e-29 | 104 |
| Msa1386690 | MtrunA17_Chr5g0447571 | 37.671 | 146 | 56 | 7 | 1 | 143 | 1 | 114 | 9.76e-15 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCATGTTTCTCTTTCTCTT+TGG | 0.220719 | tig0020858:+18483 | Msa1386690:CDS |
| TGTGACTCTCCCTCTGATTC+TGG | 0.237557 | tig0020858:+18632 | Msa1386690:CDS |
| ACAAGAGAGGTTTAGACAAT+TGG | 0.347052 | tig0020858:+18304 | Msa1386690:CDS |
| GAAGAGTGAACAACAATAGT+AGG | 0.358947 | tig0020858:-18269 | None:intergenic |
| GTATTTGAATTGAATTGTTT+TGG | 0.359597 | tig0020858:-18380 | None:intergenic |
| GTGCATAAGTTCATTGAAAC+TGG | 0.364634 | tig0020858:-18560 | None:intergenic |
| TTGCAGGCTTCATGGAAGAA+TGG | 0.498232 | tig0020858:+18602 | Msa1386690:CDS |
| ACATGAGGTGGTGACCTAGA+TGG | 0.507903 | tig0020858:-18467 | None:intergenic |
| TAGATGGTATGATCAATTCA+TGG | 0.508899 | tig0020858:-18451 | None:intergenic |
| GATTGATCATAATCATAACT+TGG | 0.513085 | tig0020858:-18407 | None:intergenic |
| ATGAATTGATCATACCATCT+AGG | 0.523755 | tig0020858:+18453 | Msa1386690:CDS |
| ATCATAATCATAACTTGGAA+TGG | 0.534200 | tig0020858:-18402 | None:intergenic |
| AGTGTCAACACCAGAATCAG+AGG | 0.544827 | tig0020858:-18642 | None:intergenic |
| CCTCTCTTGTAGAAGTGCAA+TGG | 0.554238 | tig0020858:-18291 | None:intergenic |
| CCATTGCACTTCTACAAGAG+AGG | 0.559570 | tig0020858:+18291 | Msa1386690:CDS |
| ACCACAAACATATAATGGGA+AGG | 0.573707 | tig0020858:+18234 | None:intergenic |
| ATCAGAGGTAAAACAACAAG+AGG | 0.584381 | tig0020858:+18514 | Msa1386690:CDS |
| GAACAACAATAGTAGGGTCA+TGG | 0.596576 | tig0020858:-18262 | None:intergenic |
| AAGAGTGAACAACAATAGTA+GGG | 0.597178 | tig0020858:-18268 | None:intergenic |
| TGGTATGATCAATTCATGGT+TGG | 0.604766 | tig0020858:-18447 | None:intergenic |
| CCACAAACATATAATGGGAA+GGG | 0.610714 | tig0020858:+18235 | None:intergenic |
| TCTTTGGCCAACAACATCAG+AGG | 0.623302 | tig0020858:+18499 | Msa1386690:CDS |
| GTGTCAACACCAGAATCAGA+GGG | 0.640282 | tig0020858:-18641 | None:intergenic |
| AGAGAAAGAGAAACATGAGG+TGG | 0.690008 | tig0020858:-18479 | None:intergenic |
| CAAAGAGAAAGAGAAACATG+AGG | 0.718120 | tig0020858:-18482 | None:intergenic |
| ATGATTATGATCAATCAACA+AGG | 0.724176 | tig0020858:+18414 | Msa1386690:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTATTTGAATTGAATTGTTT+TGG | - | tig0020858:18383-18402 | None:intergenic | 20.0% |
| !!! | CAAGTTTTTTGTATAATTTG+CGG | - | tig0020858:18585-18604 | None:intergenic | 20.0% |
| !! | AAATTATACAAAAAACTTGC+AGG | + | tig0020858:18586-18605 | Msa1386690:CDS | 20.0% |
| ! | ATCATAATCATAACTTGGAA+TGG | - | tig0020858:18405-18424 | None:intergenic | 25.0% |
| ! | GATTGATCATAATCATAACT+TGG | - | tig0020858:18410-18429 | None:intergenic | 25.0% |
| ! | ATGATTATGATCAATCAACA+AGG | + | tig0020858:18414-18433 | Msa1386690:CDS | 25.0% |
| AAGAGTGAACAACAATAGTA+GGG | - | tig0020858:18271-18290 | None:intergenic | 30.0% | |
| TAGATGGTATGATCAATTCA+TGG | - | tig0020858:18454-18473 | None:intergenic | 30.0% | |
| ATGAATTGATCATACCATCT+AGG | + | tig0020858:18453-18472 | Msa1386690:CDS | 30.0% | |
| GAAGAGTGAACAACAATAGT+AGG | - | tig0020858:18272-18291 | None:intergenic | 35.0% | |
| ACAAGAGAGGTTTAGACAAT+TGG | + | tig0020858:18304-18323 | Msa1386690:CDS | 35.0% | |
| TGGTATGATCAATTCATGGT+TGG | - | tig0020858:18450-18469 | None:intergenic | 35.0% | |
| CAAAGAGAAAGAGAAACATG+AGG | - | tig0020858:18485-18504 | None:intergenic | 35.0% | |
| CTCATGTTTCTCTTTCTCTT+TGG | + | tig0020858:18483-18502 | Msa1386690:CDS | 35.0% | |
| ! | TGTTTTACCTCTGATGTTGT+TGG | - | tig0020858:18509-18528 | None:intergenic | 35.0% |
| ATCAGAGGTAAAACAACAAG+AGG | + | tig0020858:18514-18533 | Msa1386690:CDS | 35.0% | |
| GTGCATAAGTTCATTGAAAC+TGG | - | tig0020858:18563-18582 | None:intergenic | 35.0% | |
| GAACAACAATAGTAGGGTCA+TGG | - | tig0020858:18265-18284 | None:intergenic | 40.0% | |
| AGAGAAAGAGAAACATGAGG+TGG | - | tig0020858:18482-18501 | None:intergenic | 40.0% | |
| CAAAAAACTTGCAGGCTTCA+TGG | + | tig0020858:18594-18613 | Msa1386690:CDS | 40.0% | |
| ! | CCTCTCTTGTAGAAGTGCAA+TGG | - | tig0020858:18294-18313 | None:intergenic | 45.0% |
| CCATTGCACTTCTACAAGAG+AGG | + | tig0020858:18291-18310 | Msa1386690:CDS | 45.0% | |
| TCTTTGGCCAACAACATCAG+AGG | + | tig0020858:18499-18518 | Msa1386690:CDS | 45.0% | |
| TTGCAGGCTTCATGGAAGAA+TGG | + | tig0020858:18602-18621 | Msa1386690:CDS | 45.0% | |
| GTGTCAACACCAGAATCAGA+GGG | - | tig0020858:18644-18663 | None:intergenic | 45.0% | |
| AGTGTCAACACCAGAATCAG+AGG | - | tig0020858:18645-18664 | None:intergenic | 45.0% | |
| ACATGAGGTGGTGACCTAGA+TGG | - | tig0020858:18470-18489 | None:intergenic | 50.0% | |
| TGTGACTCTCCCTCTGATTC+TGG | + | tig0020858:18632-18651 | Msa1386690:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0020858 | gene | 18248 | 18679 | 18248 | ID=Msa1386690;Name=Msa1386690 |
| tig0020858 | mRNA | 18248 | 18679 | 18248 | ID=Msa1386690-mRNA-1;Parent=Msa1386690;Name=Msa1386690-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|143 |
| tig0020858 | exon | 18248 | 18679 | 18248 | ID=Msa1386690-mRNA-1:exon:10306;Parent=Msa1386690-mRNA-1 |
| tig0020858 | CDS | 18248 | 18679 | 18248 | ID=Msa1386690-mRNA-1:cds;Parent=Msa1386690-mRNA-1 |
| Gene Sequence |
| Protein sequence |
