AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1402390

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1402390 MtrunA17_Chr7g0268501 95.161 186 9 0 1 186 1 186 8.27e-132 372
Msa1402390 MtrunA17_Chr7g0268521 95.161 186 9 0 1 186 1 186 8.27e-132 372
Msa1402390 MtrunA17_Chr4g0024121 51.832 191 86 3 1 186 1 190 1.90e-62 196
Msa1402390 MtrunA17_Chr2g0322861 52.083 192 82 4 1 186 62 249 4.06e-61 194
Msa1402390 MtrunA17_Chr6g0460711 49.171 181 88 2 7 185 3 181 7.60e-55 176
Msa1402390 MtrunA17_Chr8g0371381 48.824 170 84 2 19 186 20 188 1.04e-54 176
Msa1402390 MtrunA17_Chr1g0157051 48.619 181 87 3 11 186 7 186 1.56e-54 175
Msa1402390 MtrunA17_Chr5g0405991 50.000 186 85 3 8 186 1 185 2.54e-54 175
Msa1402390 MtrunA17_Chr2g0279041 51.205 166 75 3 22 185 24 185 2.18e-52 170
Msa1402390 MtrunA17_Chr4g0063671 44.920 187 91 4 1 185 3 179 8.04e-52 168
Msa1402390 MtrunA17_Chr1g0204011 46.629 178 91 3 11 186 14 189 3.70e-51 167
Msa1402390 MtrunA17_Chr6g0477071 43.784 185 99 4 5 186 34 216 1.16e-50 166
Msa1402390 MtrunA17_Chr1g0188431 45.161 186 98 3 3 186 18 201 5.56e-49 161
Msa1402390 MtrunA17_Chr1g0213061 45.810 179 93 3 11 186 13 190 1.91e-48 160
Msa1402390 MtrunA17_Chr1g0164891 54.878 164 68 3 29 186 93 256 5.73e-48 160
Msa1402390 MtrunA17_Chr7g0254231 46.667 165 84 2 23 185 20 182 3.95e-47 156
Msa1402390 MtrunA17_Chr2g0292211 45.652 184 94 3 6 186 24 204 1.31e-46 156
Msa1402390 MtrunA17_Chr3g0128061 44.565 184 98 3 6 186 8 190 1.92e-46 155
Msa1402390 MtrunA17_Chr3g0129051 45.556 180 95 2 10 186 13 192 2.08e-46 154
Msa1402390 MtrunA17_Chr2g0320071 43.316 187 101 3 3 186 5 189 4.22e-46 154
Msa1402390 MtrunA17_Chr4g0068621 49.405 168 82 2 22 186 35 202 5.35e-46 154
Msa1402390 MtrunA17_Chr5g0404721 47.429 175 89 2 15 186 20 194 7.47e-46 153
Msa1402390 MtrunA17_Chr7g0254201 46.012 163 84 2 25 185 20 180 1.71e-45 152
Msa1402390 MtrunA17_Chr3g0114451 44.792 192 98 4 1 186 1 190 2.31e-45 152
Msa1402390 MtrunA17_Chr5g0402571 45.856 181 92 5 11 186 11 190 2.98e-45 152
Msa1402390 MtrunA17_Chr5g0402581 45.856 181 92 5 11 186 11 190 4.38e-45 151
Msa1402390 MtrunA17_Chr2g0291751 43.478 184 101 2 6 186 10 193 5.78e-45 152
Msa1402390 MtrunA17_Chr2g0292181 41.753 194 104 3 2 186 9 202 1.08e-44 151
Msa1402390 MtrunA17_Chr2g0292241 43.575 179 98 2 11 186 15 193 2.32e-44 150
Msa1402390 MtrunA17_Chr7g0258811 41.711 187 104 4 4 186 49 234 4.00e-44 150
Msa1402390 MtrunA17_Chr4g0070951 41.622 185 100 4 5 186 11 190 4.56e-44 149
Msa1402390 MtrunA17_Chr5g0432331 44.271 192 101 3 1 186 32 223 5.79e-44 149
Msa1402390 MtrunA17_Chr2g0292111 44.693 179 96 2 11 186 15 193 7.25e-44 149
Msa1402390 MtrunA17_Chr1g0175561 44.970 169 87 4 23 186 22 189 8.91e-44 148
Msa1402390 MtrunA17_Chr8g0372601 44.886 176 94 2 14 186 19 194 9.06e-44 148
Msa1402390 MtrunA17_Chr2g0292091 43.169 183 100 3 8 186 6 188 9.29e-44 148
Msa1402390 MtrunA17_Chr2g0292271 45.000 180 94 3 12 186 14 193 1.12e-43 148
Msa1402390 MtrunA17_Chr2g0292251 45.882 170 89 2 20 186 23 192 1.63e-43 148
Msa1402390 MtrunA17_Chr5g0432351 43.229 192 103 4 1 186 1 192 2.37e-43 147
Msa1402390 MtrunA17_Chr2g0320101 41.530 183 104 2 7 186 7 189 3.83e-43 146
Msa1402390 MtrunA17_Chr5g0400331 50.311 161 77 2 29 186 30 190 5.02e-43 146
Msa1402390 MtrunA17_Chr5g0432341 43.229 192 103 4 1 186 1 192 8.36e-43 145
Msa1402390 MtrunA17_Chr2g0316811 45.029 171 90 2 19 185 44 214 9.43e-43 145
Msa1402390 MtrunA17_Chr2g0320061 41.530 183 104 2 7 186 7 189 2.42e-42 144
Msa1402390 MtrunA17_Chr4g0043391 45.455 165 87 2 25 186 27 191 3.12e-42 144
Msa1402390 MtrunA17_Chr2g0316481 45.029 171 90 2 19 185 70 240 3.19e-42 145
Msa1402390 MtrunA17_Chr2g0316831 45.029 171 90 2 19 185 106 276 4.11e-42 146
Msa1402390 MtrunA17_Chr5g0432441 42.781 187 97 5 10 186 9 195 4.55e-42 143
Msa1402390 MtrunA17_Chr5g0438021 43.455 191 97 4 6 186 6 195 5.17e-42 143
Msa1402390 MtrunA17_Chr2g0316791 45.029 171 90 2 19 185 70 240 5.70e-42 145
Msa1402390 MtrunA17_Chr2g0320051 40.984 183 105 2 7 186 7 189 9.72e-42 142
Msa1402390 MtrunA17_Chr1g0194271 42.051 195 104 3 1 186 2 196 1.38e-41 142
Msa1402390 MtrunA17_Chr2g0292261 43.889 180 96 4 11 186 15 193 2.23e-41 142
Msa1402390 MtrunA17_Chr4g0013721 44.118 170 92 2 20 186 95 264 5.26e-41 142
Msa1402390 MtrunA17_Chr2g0316191 44.444 171 91 2 19 185 70 240 5.54e-41 142
Msa1402390 MtrunA17_Chr2g0292151 43.575 179 98 2 11 186 15 193 7.11e-41 141
Msa1402390 MtrunA17_Chr4g0013821 43.636 165 90 2 25 186 28 192 7.21e-41 140
Msa1402390 MtrunA17_Chr2g0320031 41.489 188 104 4 3 186 5 190 7.42e-41 140
Msa1402390 MtrunA17_Chr4g0038171 44.848 165 88 2 25 186 24 188 1.09e-40 140
Msa1402390 MtrunA17_Chr4g0046131 45.833 168 85 3 25 186 42 209 1.94e-40 139
Msa1402390 MtrunA17_Chr7g0244451 41.799 189 106 3 1 186 1 188 2.34e-40 139
Msa1402390 MtrunA17_Chr2g0292101 41.848 184 103 3 7 186 6 189 2.73e-40 139
Msa1402390 MtrunA17_Chr2g0320091 40.984 183 105 2 7 186 7 189 2.73e-40 139
Msa1402390 MtrunA17_Chr3g0106921 41.530 183 102 4 8 186 9 190 3.61e-40 138
Msa1402390 MtrunA17_Chr3g0129061 44.444 180 97 2 10 186 13 192 9.64e-40 137
Msa1402390 MtrunA17_Chr1g0194151 42.541 181 99 2 11 186 16 196 1.05e-39 137
Msa1402390 MtrunA17_Chr3g0129041 45.000 180 96 2 10 186 13 192 1.63e-39 137
Msa1402390 MtrunA17_Chr7g0224371 39.683 189 111 2 1 186 7 195 1.81e-39 136
Msa1402390 MtrunA17_Chr2g0278671 46.667 165 81 4 25 186 24 184 6.37e-39 135
Msa1402390 MtrunA17_Chr5g0432401 42.135 178 97 3 4 175 8 185 1.34e-38 133
Msa1402390 MtrunA17_Chr2g0296621 45.342 161 82 3 28 186 53 209 1.61e-38 134
Msa1402390 MtrunA17_Chr2g0311251 44.444 171 90 3 19 185 5 174 1.63e-38 134
Msa1402390 MtrunA17_Chr4g0038161 39.362 188 110 3 3 186 1 188 1.85e-38 134
Msa1402390 MtrunA17_Chr7g0225221 40.741 189 106 4 2 186 3 189 4.47e-38 133
Msa1402390 MtrunA17_Chr4g0013171 42.857 168 91 4 24 186 26 193 5.89e-38 132
Msa1402390 MtrunA17_Chr2g0311241 39.151 212 83 4 19 185 54 264 6.13e-38 135
Msa1402390 MtrunA17_Chr3g0106901 43.243 185 99 3 5 186 14 195 8.92e-38 132
Msa1402390 MtrunA17_Chr4g0038151 39.153 189 110 4 3 186 1 189 8.92e-38 132
Msa1402390 MtrunA17_Chr4g0013201 43.030 165 91 2 25 186 46 210 1.02e-37 132
Msa1402390 MtrunA17_Chr4g0013181 43.902 164 87 4 28 186 62 225 1.11e-37 132
Msa1402390 MtrunA17_Chr4g0053871 43.523 193 100 6 2 186 3 194 1.18e-37 132
Msa1402390 MtrunA17_Chr4g0013191 39.901 203 99 4 7 186 8 210 1.24e-37 132
Msa1402390 MtrunA17_Chr7g0244461 41.011 178 101 3 12 186 8 184 1.58e-37 131
Msa1402390 MtrunA17_Chr8g0364291 43.558 163 88 2 28 186 26 188 2.25e-37 131
Msa1402390 MtrunA17_Chr1g0155611 43.636 165 90 2 25 186 46 210 2.28e-37 132
Msa1402390 MtrunA17_Chr3g0107881 39.894 188 105 5 5 186 9 194 3.26e-37 130
Msa1402390 MtrunA17_Chr4g0076211 45.283 159 81 3 30 186 46 200 8.69e-37 130
Msa1402390 MtrunA17_Chr2g0299691 42.857 161 87 3 29 186 60 218 2.93e-36 129
Msa1402390 MtrunA17_Chr3g0136751 41.477 176 95 3 2 175 5 174 4.64e-36 127
Msa1402390 MtrunA17_Chr2g0292291 40.000 170 99 2 20 186 25 194 2.99e-35 126
Msa1402390 MtrunA17_Chr1g0148021 43.617 188 96 7 6 186 9 193 2.21e-34 123
Msa1402390 MtrunA17_Chr2g0279061 53.782 119 49 3 69 185 12 126 2.83e-34 122
Msa1402390 MtrunA17_Chr6g0451571 41.176 187 102 5 5 186 17 200 4.29e-34 123
Msa1402390 MtrunA17_Chr5g0413051 40.000 170 97 4 21 185 69 238 2.13e-33 122
Msa1402390 MtrunA17_Chr1g0197411 38.542 192 107 5 5 186 4 194 1.48e-32 119
Msa1402390 MtrunA17_Chr5g0421411 44.681 141 73 4 49 185 5 144 2.66e-32 117
Msa1402390 MtrunA17_Chr2g0329641 41.104 163 90 3 25 185 36 194 6.71e-32 117
Msa1402390 MtrunA17_Chr5g0401431 39.378 193 108 6 2 186 3 194 2.33e-31 115
Msa1402390 MtrunA17_Chr4g0075241 36.310 168 104 2 21 185 25 192 6.55e-31 114
Msa1402390 MtrunA17_Chr4g0072091 41.071 168 90 4 25 186 24 188 1.80e-30 113
Msa1402390 MtrunA17_Chr3g0114491 49.533 107 53 1 25 130 18 124 3.73e-30 107
Msa1402390 MtrunA17_Chr6g0467251 36.508 189 110 6 6 186 14 200 4.42e-29 110
Msa1402390 MtrunA17_Chr6g0467121 34.872 195 109 7 5 186 46 235 3.79e-27 105
Msa1402390 MtrunA17_Chr1g0196191 59.756 82 30 1 67 148 27 105 1.49e-26 99.0
Msa1402390 MtrunA17_Chr4g0074851 38.889 162 87 3 5 164 20 171 2.70e-26 102
Msa1402390 MtrunA17_Chr1g0181511 39.583 144 79 3 25 164 27 166 1.96e-25 100
Msa1402390 MtrunA17_Chr1g0181431 38.411 151 82 4 18 164 23 166 2.54e-25 99.8
Msa1402390 MtrunA17_Chr6g0467161 33.333 192 115 5 6 186 19 208 7.18e-25 98.6
Msa1402390 MtrunA17_Chr6g0468731 64.179 67 21 1 64 130 22 85 1.92e-24 91.7
Msa1402390 MtrunA17_Chr2g0316141 45.217 115 59 2 71 181 11 125 9.21e-24 94.7
Msa1402390 MtrunA17_Chr2g0316181 45.217 115 59 2 71 181 11 125 9.21e-24 94.7
Msa1402390 MtrunA17_Chr1g0181481 36.806 144 83 3 25 164 27 166 5.03e-23 93.6
Msa1402390 MtrunA17_Chr1g0181491 37.719 114 70 1 17 130 4 116 9.67e-23 89.0
Msa1402390 MtrunA17_Chr4g0004591 33.129 163 69 2 25 185 77 201 1.26e-20 87.4
Msa1402390 MtrunA17_Chr7g0254181 41.346 104 56 2 84 185 19 119 3.51e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1402390 AT1G05260.1 55.738 183 76 3 8 186 7 188 7.15e-65 202
Msa1402390 AT5G42180.1 50.000 186 90 2 3 186 1 185 4.08e-60 190
Msa1402390 AT4G11290.1 51.913 183 84 3 7 186 8 189 5.57e-60 190
Msa1402390 AT1G05240.1 54.658 161 70 2 28 186 29 188 2.05e-58 186
Msa1402390 AT1G05250.1 54.658 161 70 2 28 186 29 188 2.05e-58 186
Msa1402390 AT3G21770.1 52.571 175 78 3 16 186 18 191 3.52e-57 182
Msa1402390 AT3G01190.1 50.811 185 86 3 4 186 5 186 5.42e-55 177
Msa1402390 AT2G39040.1 51.786 168 77 2 23 186 44 211 2.21e-54 176
Msa1402390 AT5G15180.1 52.121 165 76 2 24 186 31 194 1.59e-53 173
Msa1402390 AT5G51890.1 49.112 169 75 4 24 186 25 188 1.37e-51 168
Msa1402390 AT2G41480.1 49.080 163 77 3 25 185 29 187 3.33e-50 165
Msa1402390 AT4G33420.1 46.739 184 92 4 8 186 17 199 6.56e-50 164
Msa1402390 AT4G33420.2 46.739 184 92 4 8 186 64 246 2.37e-49 164
Msa1402390 AT3G03670.1 44.149 188 99 3 1 185 1 185 4.25e-49 162
Msa1402390 AT4G26010.1 43.094 181 99 2 7 185 4 182 3.30e-46 154
Msa1402390 AT4G26010.2 43.094 181 99 2 7 185 32 210 6.81e-46 154
Msa1402390 AT5G66390.1 45.714 175 92 2 15 186 23 197 1.35e-45 153
Msa1402390 AT5G17820.1 43.784 185 97 3 3 185 2 181 1.76e-45 152
Msa1402390 AT5G64100.1 49.375 160 74 3 28 185 40 194 1.86e-45 152
Msa1402390 AT5G39580.2 42.162 185 100 4 3 185 4 183 1.87e-45 150
Msa1402390 AT4G36430.1 44.134 179 97 2 11 186 16 194 4.57e-45 152
Msa1402390 AT1G44970.1 47.977 173 87 2 17 186 37 209 5.68e-45 152
Msa1402390 AT5G39580.1 42.162 185 100 4 3 185 4 183 8.66e-45 150
Msa1402390 AT5G64120.1 46.914 162 80 3 26 185 36 193 8.92e-45 150
Msa1402390 AT5G17820.2 43.784 185 97 3 3 185 55 234 1.22e-44 151
Msa1402390 AT5G06720.1 40.541 185 107 2 5 186 12 196 5.28e-44 149
Msa1402390 AT2G18150.1 45.509 167 88 2 23 186 34 200 1.55e-43 148
Msa1402390 AT2G24800.2 50.000 164 77 2 25 186 31 191 1.73e-43 145
Msa1402390 AT5G05340.1 44.242 165 89 2 25 186 30 194 4.71e-43 146
Msa1402390 AT2G38380.1 42.703 185 100 4 8 186 12 196 7.33e-43 146
Msa1402390 AT4G25980.2 46.243 173 87 4 16 186 63 231 8.32e-43 144
Msa1402390 AT2G35380.1 50.000 168 81 2 22 186 27 194 1.65e-42 145
Msa1402390 AT4G31760.1 44.944 178 93 2 11 186 15 189 3.10e-42 144
Msa1402390 AT4G25980.1 46.243 173 87 4 16 186 18 186 3.17e-42 144
Msa1402390 AT2G24800.1 50.000 164 77 2 25 186 31 191 5.31e-42 144
Msa1402390 AT1G77100.1 46.341 164 84 3 25 186 24 185 6.56e-42 143
Msa1402390 AT5G22410.1 42.077 183 96 4 7 185 11 187 1.11e-41 143
Msa1402390 AT5G06730.1 40.000 185 108 2 5 186 13 197 1.39e-41 143
Msa1402390 AT4G16270.1 45.963 161 84 2 29 186 54 214 2.21e-40 140
Msa1402390 AT2G18140.1 42.690 171 92 3 22 186 29 199 2.21e-40 139
Msa1402390 AT5G19890.1 46.061 165 82 4 25 186 30 190 3.20e-40 139
Msa1402390 AT3G50990.1 43.976 166 90 2 23 185 39 204 3.35e-40 139
Msa1402390 AT5G67400.1 44.041 193 97 6 4 186 3 194 4.31e-40 139
Msa1402390 AT4G37520.1 46.746 169 82 5 25 186 27 194 6.13e-40 138
Msa1402390 AT3G32980.1 43.407 182 97 4 11 186 15 196 1.13e-39 138
Msa1402390 AT2G38390.1 40.000 185 105 4 8 186 12 196 1.49e-39 138
Msa1402390 AT3G49960.1 46.746 169 82 5 25 186 27 194 1.90e-39 137
Msa1402390 AT4G37520.2 46.707 167 82 5 25 186 27 191 3.91e-39 136
Msa1402390 AT1G49570.1 43.373 166 91 2 24 186 47 212 6.96e-39 136
Msa1402390 AT1G14550.1 43.452 168 87 4 25 186 26 191 7.25e-39 135
Msa1402390 AT1G68850.1 38.710 186 109 3 5 185 8 193 8.24e-39 135
Msa1402390 AT5G14130.1 44.974 189 94 7 3 186 13 196 9.78e-39 135
Msa1402390 AT3G28200.1 45.181 166 87 2 25 186 21 186 1.23e-38 134
Msa1402390 AT4G37530.2 44.970 169 85 5 25 186 27 194 1.38e-38 134
Msa1402390 AT5G14130.2 44.974 189 94 7 3 186 54 237 2.40e-38 135
Msa1402390 AT4G37530.1 44.970 169 85 5 25 186 27 194 3.98e-38 134
Msa1402390 AT5G64110.1 44.785 163 83 3 26 186 35 192 7.62e-38 133
Msa1402390 AT4G08770.1 43.373 166 90 3 25 186 24 189 8.54e-38 133
Msa1402390 AT2G22420.1 41.667 168 95 2 22 186 20 187 1.24e-37 132
Msa1402390 AT4G33870.1 40.113 177 101 2 14 185 54 230 1.30e-37 134
Msa1402390 AT4G08780.1 43.373 166 90 3 25 186 24 189 2.89e-37 132
Msa1402390 AT1G71695.1 44.048 168 87 5 25 186 44 210 2.92e-37 132
Msa1402390 AT3G49120.1 42.353 170 94 3 21 186 28 197 8.06e-37 130
Msa1402390 AT4G17690.1 41.916 167 93 2 24 186 25 191 1.29e-36 129
Msa1402390 AT3G49110.1 42.771 166 91 3 25 186 33 198 2.06e-36 130
Msa1402390 AT5G47000.1 37.838 185 106 3 11 186 14 198 1.70e-35 127
Msa1402390 AT5G58400.1 40.541 185 103 4 9 186 11 195 2.12e-35 126
Msa1402390 AT1G14540.1 39.344 183 104 3 7 186 7 185 4.85e-35 125
Msa1402390 AT5G40150.1 42.771 166 91 2 25 186 32 197 9.42e-35 125
Msa1402390 AT2G34060.1 42.857 168 90 3 25 186 42 209 2.14e-34 124
Msa1402390 AT1G34510.1 37.569 181 109 2 7 185 4 182 2.96e-34 123
Msa1402390 AT2G18980.1 41.489 188 98 5 5 186 7 188 3.53e-34 123
Msa1402390 AT5G19880.1 40.909 176 99 3 16 186 16 191 3.80e-34 123
Msa1402390 AT5G58390.1 42.771 166 91 3 25 186 21 186 2.50e-33 120
Msa1402390 AT2G43480.1 41.520 171 88 5 22 185 29 194 1.86e-32 119
Msa1402390 AT4G30170.1 39.286 168 92 4 25 186 27 190 6.21e-32 117
Msa1402390 AT5G24070.1 37.654 162 92 4 28 185 38 194 1.43e-29 111
Msa1402390 AT1G30870.1 41.104 163 86 4 28 186 54 210 1.56e-29 111
Msa1402390 AT1G24110.1 38.378 185 103 3 5 185 9 186 2.78e-29 110
Msa1402390 AT4G21960.1 36.898 187 111 2 6 185 7 193 1.02e-27 106
Msa1402390 AT3G17070.1 35.638 188 112 5 6 185 16 202 3.52e-27 105
Msa1402390 AT2G37130.1 38.095 147 82 3 23 164 28 170 1.93e-25 100
Msa1402390 AT2G37130.2 38.028 142 79 3 28 164 2 139 2.21e-24 97.1
Msa1402390 AT2G35380.2 47.115 104 52 2 86 186 3 106 2.46e-18 80.1

Find 40 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGACTTGGTTGTATTATC+AGG 0.207951 tig0022876:-36 None:intergenic
ATTCTTCCTTGTAGCTTCTA+TGG 0.251513 tig0022876:-786 Msa1402390:CDS
AAGCCTGCTAATGATAAATT+AGG 0.273981 tig0022876:+391 None:intergenic
GATTAGGGCTTCAATGCTTC+TGG 0.289264 tig0022876:+128 None:intergenic
ATCAACTTGGCTGGCAGCTC+AGG 0.326426 tig0022876:+641 None:intergenic
AGAAGACATAAAAGAAGCTT+TGG 0.382183 tig0022876:-359 Msa1402390:CDS
AAGGTGCTGGAATATTGATT+AGG 0.388624 tig0022876:+112 None:intergenic
AGGTGCTGGAATATTGATTA+GGG 0.399370 tig0022876:+113 None:intergenic
CCCTACAACATGTCTCTTCA+AGG 0.409923 tig0022876:-664 Msa1402390:CDS
GTCTGAGCTGAGTGATGTTA+TGG 0.410714 tig0022876:+88 None:intergenic
AGCAACGTGGGAAGTGCTTA+CGG 0.416975 tig0022876:-172 Msa1402390:CDS
TCAGCACAGGATACAATTCC+AGG 0.433326 tig0022876:+325 None:intergenic
ATTCCTAATTTATCATTAGC+AGG 0.434054 tig0022876:-394 Msa1402390:CDS
TTAGTGAAGGAGGCAGCCTC+AGG 0.457227 tig0022876:-742 Msa1402390:CDS
GCAACGTGGGAAGTGCTTAC+GGG 0.476545 tig0022876:-171 Msa1402390:CDS
AGACATAAAAGAAGCTTTGG+AGG 0.490687 tig0022876:-356 Msa1402390:CDS
TGCTGGTAACACTGCGGAGA+AGG 0.493705 tig0022876:-422 Msa1402390:CDS
AAAGCTTACTCTTCATGACT+TGG 0.493798 tig0022876:-49 Msa1402390:CDS
TGCAGTCTTATCAACTTGGC+TGG 0.499023 tig0022876:+632 None:intergenic
GCTTTGGAGGAGAAATGTCC+TGG 0.501858 tig0022876:-343 Msa1402390:CDS
GTTTCAGGGGTGTGACGCAT+CGG 0.508181 tig0022876:-461 Msa1402390:intron
CAATGTGAGTATATCAGCAC+AGG 0.523102 tig0022876:+312 None:intergenic
TGTTTCATTGGGATTAGTGA+AGG 0.528117 tig0022876:-755 Msa1402390:CDS
CTCCGCAGTGTTACCAGCAG+TGG 0.544816 tig0022876:+426 None:intergenic
TTAACAATCTCCTCAGCTTG+AGG 0.546103 tig0022876:+695 None:intergenic
AAAATGCAGTCTTATCAACT+TGG 0.547582 tig0022876:+628 None:intergenic
CCTTGAAGAGACATGTTGTA+GGG 0.548319 tig0022876:+664 None:intergenic
GCCTTGAAGAGACATGTTGT+AGG 0.550855 tig0022876:+663 None:intergenic
TGCTAATGATAAATTAGGAA+TGG 0.579765 tig0022876:+396 None:intergenic
TCTTATAGAAATTCTTCCTG+AGG 0.587628 tig0022876:+726 None:intergenic
GTGATGTTATGGAAAGGTGC+TGG 0.588441 tig0022876:+99 None:intergenic
GTGCTTACGGGTAGAAGAGA+TGG 0.590073 tig0022876:-159 Msa1402390:CDS
AAAGCACCATAGAAGCTACA+AGG 0.611637 tig0022876:+780 None:intergenic
TTCATTGGGATTAGTGAAGG+AGG 0.626140 tig0022876:-752 Msa1402390:CDS
GTTTAATGATAAAGCAACGT+GGG 0.643577 tig0022876:-184 Msa1402390:CDS
CTGTGCTGATATACTCACAT+TGG 0.650535 tig0022876:-311 Msa1402390:CDS
GAAGTCATGTCCTCAAGCTG+AGG 0.657533 tig0022876:-705 Msa1402390:CDS
AGCTGAGTGATGTTATGGAA+AGG 0.700700 tig0022876:+93 None:intergenic
AGTTTAATGATAAAGCAACG+TGG 0.776571 tig0022876:-185 Msa1402390:CDS
GTCCACTGCTGGTAACACTG+CGG 0.786302 tig0022876:-428 Msa1402390:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAGGAATATGTATAAATTAA+TGG + tig0022876:589-608 None:intergenic 15.0%
!! AAAATATGAATGAATAAATC+AGG + tig0022876:624-643 None:intergenic 15.0%
!! TAGGAATATGTATAAATTAA+TGG + tig0022876:589-608 None:intergenic 15.0%
!! AAAATATGAATGAATAAATC+AGG + tig0022876:624-643 None:intergenic 15.0%
!!! ATTTTCATGACTGTTTTGTT+AGG - tig0022876:225-244 Msa1402390:intron 25.0%
!!! TTTTCATGACTGTTTTGTTA+GGG - tig0022876:226-245 Msa1402390:intron 25.0%
! CTAAAATTGTAAAGATCAAG+GGG + tig0022876:258-277 None:intergenic 25.0%
! GCTAAAATTGTAAAGATCAA+GGG + tig0022876:259-278 None:intergenic 25.0%
! AGCTAAAATTGTAAAGATCA+AGG + tig0022876:260-279 None:intergenic 25.0%
! TGCTAATGATAAATTAGGAA+TGG + tig0022876:442-461 None:intergenic 25.0%
! ATTCCTAATTTATCATTAGC+AGG - tig0022876:441-460 Msa1402390:CDS 25.0%
!!! ATTTTCATGACTGTTTTGTT+AGG - tig0022876:225-244 Msa1402390:intron 25.0%
!!! TTTTCATGACTGTTTTGTTA+GGG - tig0022876:226-245 Msa1402390:intron 25.0%
! CTAAAATTGTAAAGATCAAG+GGG + tig0022876:258-277 None:intergenic 25.0%
! GCTAAAATTGTAAAGATCAA+GGG + tig0022876:259-278 None:intergenic 25.0%
! AGCTAAAATTGTAAAGATCA+AGG + tig0022876:260-279 None:intergenic 25.0%
! TGCTAATGATAAATTAGGAA+TGG + tig0022876:442-461 None:intergenic 25.0%
! ATTCCTAATTTATCATTAGC+AGG - tig0022876:441-460 Msa1402390:CDS 25.0%
TCTTATAGAAATTCTTCCTG+AGG + tig0022876:112-131 None:intergenic 30.0%
! AAAATGCAGTCTTATCAACT+TGG + tig0022876:210-229 None:intergenic 30.0%
TACTAAGTGACATGCATATA+TGG - tig0022876:347-366 Msa1402390:CDS 30.0%
AAGCCTGCTAATGATAAATT+AGG + tig0022876:447-466 None:intergenic 30.0%
AGAAGACATAAAAGAAGCTT+TGG - tig0022876:476-495 Msa1402390:intron 30.0%
AGTTTAATGATAAAGCAACG+TGG - tig0022876:650-669 Msa1402390:CDS 30.0%
GTTTAATGATAAAGCAACGT+GGG - tig0022876:651-670 Msa1402390:CDS 30.0%
TCTTATAGAAATTCTTCCTG+AGG + tig0022876:112-131 None:intergenic 30.0%
! AAAATGCAGTCTTATCAACT+TGG + tig0022876:210-229 None:intergenic 30.0%
TACTAAGTGACATGCATATA+TGG - tig0022876:347-366 Msa1402390:CDS 30.0%
AAGCCTGCTAATGATAAATT+AGG + tig0022876:447-466 None:intergenic 30.0%
AGAAGACATAAAAGAAGCTT+TGG - tig0022876:476-495 Msa1402390:intron 30.0%
AGTTTAATGATAAAGCAACG+TGG - tig0022876:650-669 Msa1402390:CDS 30.0%
GTTTAATGATAAAGCAACGT+GGG - tig0022876:651-670 Msa1402390:CDS 30.0%
ATTCTTCCTTGTAGCTTCTA+TGG - tig0022876:49-68 Msa1402390:CDS 35.0%
! ATGGTGCTTTTCTGTTTCAT+TGG - tig0022876:68-87 Msa1402390:CDS 35.0%
! TGGTGCTTTTCTGTTTCATT+GGG - tig0022876:69-88 Msa1402390:CDS 35.0%
! TGTTTCATTGGGATTAGTGA+AGG - tig0022876:80-99 Msa1402390:CDS 35.0%
! AGACATAAAAGAAGCTTTGG+AGG - tig0022876:479-498 Msa1402390:intron 35.0%
!! AGGTGCTGGAATATTGATTA+GGG + tig0022876:725-744 None:intergenic 35.0%
!! AAGGTGCTGGAATATTGATT+AGG + tig0022876:726-745 None:intergenic 35.0%
AAAGCTTACTCTTCATGACT+TGG - tig0022876:786-805 Msa1402390:CDS 35.0%
ATTCTTCCTTGTAGCTTCTA+TGG - tig0022876:49-68 Msa1402390:CDS 35.0%
! ATGGTGCTTTTCTGTTTCAT+TGG - tig0022876:68-87 Msa1402390:CDS 35.0%
! TGGTGCTTTTCTGTTTCATT+GGG - tig0022876:69-88 Msa1402390:CDS 35.0%
! TGTTTCATTGGGATTAGTGA+AGG - tig0022876:80-99 Msa1402390:CDS 35.0%
! AGACATAAAAGAAGCTTTGG+AGG - tig0022876:479-498 Msa1402390:intron 35.0%
!! AGGTGCTGGAATATTGATTA+GGG + tig0022876:725-744 None:intergenic 35.0%
!! AAGGTGCTGGAATATTGATT+AGG + tig0022876:726-745 None:intergenic 35.0%
AAAGCTTACTCTTCATGACT+TGG - tig0022876:786-805 Msa1402390:CDS 35.0%
! AAAGCACCATAGAAGCTACA+AGG + tig0022876:58-77 None:intergenic 40.0%
! TTCATTGGGATTAGTGAAGG+AGG - tig0022876:83-102 Msa1402390:CDS 40.0%
TTAACAATCTCCTCAGCTTG+AGG + tig0022876:143-162 None:intergenic 40.0%
CCTTGAAGAGACATGTTGTA+GGG + tig0022876:174-193 None:intergenic 40.0%
TGCATATATGGTTGCGTTTC+AGG - tig0022876:359-378 Msa1402390:CDS 40.0%
GCATATATGGTTGCGTTTCA+GGG - tig0022876:360-379 Msa1402390:CDS 40.0%
CATATATGGTTGCGTTTCAG+GGG - tig0022876:361-380 Msa1402390:CDS 40.0%
CAATGTGAGTATATCAGCAC+AGG + tig0022876:526-545 None:intergenic 40.0%
! CTGTGCTGATATACTCACAT+TGG - tig0022876:524-543 Msa1402390:intron 40.0%
AATCAGGATGCTTCATGCAT+AGG + tig0022876:608-627 None:intergenic 40.0%
AGCTGAGTGATGTTATGGAA+AGG + tig0022876:745-764 None:intergenic 40.0%
! AAAGCACCATAGAAGCTACA+AGG + tig0022876:58-77 None:intergenic 40.0%
! TTCATTGGGATTAGTGAAGG+AGG - tig0022876:83-102 Msa1402390:CDS 40.0%
TTAACAATCTCCTCAGCTTG+AGG + tig0022876:143-162 None:intergenic 40.0%
CCTTGAAGAGACATGTTGTA+GGG + tig0022876:174-193 None:intergenic 40.0%
TGCATATATGGTTGCGTTTC+AGG - tig0022876:359-378 Msa1402390:CDS 40.0%
GCATATATGGTTGCGTTTCA+GGG - tig0022876:360-379 Msa1402390:CDS 40.0%
CATATATGGTTGCGTTTCAG+GGG - tig0022876:361-380 Msa1402390:CDS 40.0%
CAATGTGAGTATATCAGCAC+AGG + tig0022876:526-545 None:intergenic 40.0%
! CTGTGCTGATATACTCACAT+TGG - tig0022876:524-543 Msa1402390:intron 40.0%
AATCAGGATGCTTCATGCAT+AGG + tig0022876:608-627 None:intergenic 40.0%
AGCTGAGTGATGTTATGGAA+AGG + tig0022876:745-764 None:intergenic 40.0%
CCCTACAACATGTCTCTTCA+AGG - tig0022876:171-190 Msa1402390:CDS 45.0%
GCCTTGAAGAGACATGTTGT+AGG + tig0022876:175-194 None:intergenic 45.0%
! TGCAGTCTTATCAACTTGGC+TGG + tig0022876:206-225 None:intergenic 45.0%
TCAGCACAGGATACAATTCC+AGG + tig0022876:513-532 None:intergenic 45.0%
GATTAGGGCTTCAATGCTTC+TGG + tig0022876:710-729 None:intergenic 45.0%
! GTGATGTTATGGAAAGGTGC+TGG + tig0022876:739-758 None:intergenic 45.0%
GTCTGAGCTGAGTGATGTTA+TGG + tig0022876:750-769 None:intergenic 45.0%
CCCTACAACATGTCTCTTCA+AGG - tig0022876:171-190 Msa1402390:CDS 45.0%
GCCTTGAAGAGACATGTTGT+AGG + tig0022876:175-194 None:intergenic 45.0%
! TGCAGTCTTATCAACTTGGC+TGG + tig0022876:206-225 None:intergenic 45.0%
TCAGCACAGGATACAATTCC+AGG + tig0022876:513-532 None:intergenic 45.0%
GATTAGGGCTTCAATGCTTC+TGG + tig0022876:710-729 None:intergenic 45.0%
! GTGATGTTATGGAAAGGTGC+TGG + tig0022876:739-758 None:intergenic 45.0%
GTCTGAGCTGAGTGATGTTA+TGG + tig0022876:750-769 None:intergenic 45.0%
GAAGTCATGTCCTCAAGCTG+AGG - tig0022876:130-149 Msa1402390:CDS 50.0%
!!! GTGTGACGCATCGGTTTTGT+TGG - tig0022876:383-402 Msa1402390:CDS 50.0%
!! GTTTTGTTGGAGTCCACTGC+TGG - tig0022876:396-415 Msa1402390:CDS 50.0%
!! GCTTTGGAGGAGAAATGTCC+TGG - tig0022876:492-511 Msa1402390:intron 50.0%
!! AGCAACGTGGGAAGTGCTTA+CGG - tig0022876:663-682 Msa1402390:CDS 50.0%
GTGCTTACGGGTAGAAGAGA+TGG - tig0022876:676-695 Msa1402390:CDS 50.0%
GAAGTCATGTCCTCAAGCTG+AGG - tig0022876:130-149 Msa1402390:CDS 50.0%
!!! GTGTGACGCATCGGTTTTGT+TGG - tig0022876:383-402 Msa1402390:CDS 50.0%
!! GTTTTGTTGGAGTCCACTGC+TGG - tig0022876:396-415 Msa1402390:CDS 50.0%
!! GCTTTGGAGGAGAAATGTCC+TGG - tig0022876:492-511 Msa1402390:intron 50.0%
!! AGCAACGTGGGAAGTGCTTA+CGG - tig0022876:663-682 Msa1402390:CDS 50.0%
GTGCTTACGGGTAGAAGAGA+TGG - tig0022876:676-695 Msa1402390:CDS 50.0%
TTAGTGAAGGAGGCAGCCTC+AGG - tig0022876:93-112 Msa1402390:CDS 55.0%
ATCAACTTGGCTGGCAGCTC+AGG + tig0022876:197-216 None:intergenic 55.0%
! GTTTCAGGGGTGTGACGCAT+CGG - tig0022876:374-393 Msa1402390:CDS 55.0%
GTCCACTGCTGGTAACACTG+CGG - tig0022876:407-426 Msa1402390:CDS 55.0%
TGCTGGTAACACTGCGGAGA+AGG - tig0022876:413-432 Msa1402390:CDS 55.0%
!! GCAACGTGGGAAGTGCTTAC+GGG - tig0022876:664-683 Msa1402390:CDS 55.0%
TTAGTGAAGGAGGCAGCCTC+AGG - tig0022876:93-112 Msa1402390:CDS 55.0%
ATCAACTTGGCTGGCAGCTC+AGG + tig0022876:197-216 None:intergenic 55.0%
! GTTTCAGGGGTGTGACGCAT+CGG - tig0022876:374-393 Msa1402390:CDS 55.0%
GTCCACTGCTGGTAACACTG+CGG - tig0022876:407-426 Msa1402390:CDS 55.0%
TGCTGGTAACACTGCGGAGA+AGG - tig0022876:413-432 Msa1402390:CDS 55.0%
!! GCAACGTGGGAAGTGCTTAC+GGG - tig0022876:664-683 Msa1402390:CDS 55.0%
CTCCGCAGTGTTACCAGCAG+TGG + tig0022876:412-431 None:intergenic 60.0%
CTCCGCAGTGTTACCAGCAG+TGG + tig0022876:412-431 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0022876 gene 38 819 38 ID=Msa1402390;Name=Msa1402390
tig0022876 mRNA 38 819 38 ID=Msa1402390-mRNA-1;Parent=Msa1402390;Name=Msa1402390-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|1|1|3|0|186
tig0022876 exon 610 819 610 ID=Msa1402390-mRNA-1:exon:18957;Parent=Msa1402390-mRNA-1
tig0022876 exon 297 476 297 ID=Msa1402390-mRNA-1:exon:18956;Parent=Msa1402390-mRNA-1
tig0022876 exon 38 205 38 ID=Msa1402390-mRNA-1:exon:18955;Parent=Msa1402390-mRNA-1
tig0022876 CDS 610 819 610 ID=Msa1402390-mRNA-1:cds;Parent=Msa1402390-mRNA-1
tig0022876 CDS 297 476 297 ID=Msa1402390-mRNA-1:cds;Parent=Msa1402390-mRNA-1
tig0022876 CDS 38 205 38 ID=Msa1402390-mRNA-1:cds;Parent=Msa1402390-mRNA-1
Gene Sequence

>Msa1402390

ATGAAAATGAAATTCTTCCTTGTAGCTTCTATGGTGCTTTTCTGTTTCATTGGGATTAGTGAAGGAGGCAGCCTCAGGAAGAATTTCTATAAGAAGTCATGTCCTCAAGCTGAGGAGATTGTTAAGAACGTAACCCTACAACATGTCTCTTCAAGGCCTGAGCTGCCAGCCAAGTTGATAAGACTGCATTTTCATGACTGTTTTGTTAGGGGGTGTGACGCATCGGTTTTGTTGGAGTCCACTGCTGGTAACACTGCGGAGAAGGATGCCATTCCTAATTTATCATTAGCAGGCTTTGATGTGATAGAAGACATAAAAGAAGCTTTGGAGGAGAAATGTCCTGGAATTGTATCCTGTGCTGATATACTCACATTGGCTACTAGAGATGCTTTTAATGATAAAGCAACGTGGGAAGTGCTTACGGGTAGAAGAGATGGTACTGTGTCCAGAAGCATTGAAGCCCTAATCAATATTCCAGCACCTTTCCATAACATCACTCAGCTCAGACAAATCTTTGCTAGTAAAAAGCTTACTCTTCATGACTTGGTTGTATTATCA
Protein sequence

>Msa1402390

MKMKFFLVASMVLFCFIGISEGGSLRKNFYKKSCPQAEEIVKNVTLQHVSSRPELPAKLIRLHFHDCFVRGCDASVLLESTAGNTAEKDAIPNLSLAGFDVIEDIKEALEEKCPGIVSCADILTLATRDAFNDKATWEVLTGRRDGTVSRSIEALINIPAPFHNITQLRQIFASKKLTLHDLVVLS