AlfalfaGEDB | Msa0108350

M. sativa cultivar zhongmu-4 / Msa0108350

KEGG: K01099|K20279
GO: GO:0003674 GO:0003824 GO:0004439 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019637 GO:0030100 GO:0030258 GO:0031323 GO:0032879 GO:0033554 GO:0034485 GO:0034593 GO:0034594 GO:0034595 GO:0042578 GO:0044237 GO:0044238 GO:0044255 GO:0046486 GO:0046488 GO:0046839 GO:0046856 GO:0048259 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051716 GO:0052866 GO:0060627 GO:0065007 GO:0071214 GO:0071470 GO:0071472 GO:0071704 GO:0104004 GO:0106019 GO:2000369 GO:2000377
PFAM: PF03372.26

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0108350	XP_003589749.1	93.137	306	7	2	1	293	1	305	0.0	577

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0108350	sp\|Q9SIS4\|IP5P9_ARATH	62.718	287	98	2	9	293	1	280	6.84e-122	358

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0108350	G7IBG6	93.137	306	7	2	1	293	1	305	0.0	577

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa0108350	0.833861	4.180476e-56	-8.615850e-47
Msa0013040	Msa0108350	0.834313	3.221509e-56	-8.615850e-47
Msa0013270	Msa0108350	0.819420	1.172314e-52	-8.615850e-47
Msa0014590	Msa0108350	0.878184	3.448388e-69	-8.615850e-47
Msa0017710	Msa0108350	0.820432	6.879152e-53	-8.615850e-47
Msa0024280	Msa0108350	0.800603	1.333863e-48	-8.615850e-47
Msa0025570	Msa0108350	0.805209	1.490529e-49	-8.615850e-47
Msa0025700	Msa0108350	0.812377	4.371606e-51	-8.615850e-47
Msa0043120	Msa0108350	0.804225	2.392059e-49	-8.615850e-47
Msa0043150	Msa0108350	0.801465	8.890708e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0108350	MtrunA17_Chr1g0165011	93.137	306	7	2	1	293	1	305	0.0	577
Msa0108350	MtrunA17_Chr8g0375751	66.084	286	78	4	9	293	7	274	1.30e-126	367
Msa0108350	MtrunA17_Chr6g0479511	46.429	280	110	3	40	293	15	280	4.67e-80	247
Msa0108350	MtrunA17_Chr7g0263111	40.134	299	130	8	10	284	11	284	3.70e-58	192
Msa0108350	MtrunA17_Chr6g0452181	42.339	248	123	7	61	293	53	295	4.96e-53	179
Msa0108350	MtrunA17_Chr8g0382571	57.937	126	50	1	171	293	219	344	6.94e-41	147
Msa0108350	MtrunA17_Chr8g0382571	48.780	82	41	1	53	134	28	108	1.45e-17	82.4
Msa0108350	MtrunA17_Chr5g0394901	55.556	126	53	1	171	293	360	485	2.13e-40	148
Msa0108350	MtrunA17_Chr5g0394901	54.667	75	33	1	60	134	90	163	2.91e-18	84.7
Msa0108350	MtrunA17_Chr1g0185281	55.556	126	53	1	171	293	294	419	3.21e-40	147

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0108350	AT2G01900.3	61.695	295	104	2	1	293	1	288	9.97e-124	360
Msa0108350	AT2G01900.2	62.718	287	98	2	9	293	1	280	5.85e-123	357
Msa0108350	AT2G01900.1	62.718	287	98	2	9	293	1	280	6.96e-123	358
Msa0108350	AT2G37440.1	44.805	308	138	6	6	283	7	312	3.28e-74	235
Msa0108350	AT2G37440.3	44.805	308	138	6	6	283	7	312	3.28e-74	235
Msa0108350	AT2G37440.2	49.587	242	87	6	66	283	1	231	5.70e-64	206
Msa0108350	AT5G65090.2	57.937	126	50	1	171	293	205	330	1.71e-41	149
Msa0108350	AT5G65090.2	48.052	77	39	1	59	135	5	80	3.09e-16	79.0
Msa0108350	AT5G65090.3	57.937	126	50	1	171	293	268	393	3.74e-41	149
Msa0108350	AT5G65090.3	44.318	88	48	1	48	135	57	143	3.71e-16	79.0

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Find 51 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATTACAGAATATCTCTTC+CGG	0.427972	1_3:-19725176	Msa0108350:CDS
AAATTCAAAATAGTGCCTTT+AGG	0.373732	1_3:+19725989	None:intergenic
AAGAAGGAGATGAGAAGCAT+AGG	0.520647	1_3:-19726051	Msa0108350:CDS
AAGATACTAAACAGGAAACT+TGG	0.508569	1_3:-19727832	Msa0108350:CDS
AAGTTCTACAGATCCTTTAT+TGG	0.258601	1_3:-19727782	Msa0108350:CDS
AATAAAGGATCTGTAGAACT+TGG	0.492368	1_3:+19727784	None:intergenic
AATACATTTGATGCATTTAG+TGG	0.341530	1_3:+19727083	None:intergenic
ACACATGGATGTCTAATGAA+AGG	0.608846	1_3:+19726801	None:intergenic
ACTAAATGCATCAAATGTAT+TGG	0.366321	1_3:-19727081	Msa0108350:CDS
ACTATGATCATTAATGGATT+TGG	0.307634	1_3:+19727726	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGATACTAAACAGGAAACT+TGG	-	chr1_3:19725325-19725344	Msa0108350:intron	30.0%
AATAAAGGATCTGTAGAACT+TGG	+	chr1_3:19725376-19725395	None:intergenic	30.0%
AAGTTCTACAGATCCTTTAT+TGG	-	chr1_3:19725375-19725394	Msa0108350:intron	30.0%
TTTGTTAGTACATGGAACAT+AGG	-	chr1_3:19725627-19725646	Msa0108350:intron	30.0%
CCCAAATACATAGATATCAA+AGG	+	chr1_3:19725712-19725731	None:intergenic	30.0%
TGCATGTCCATATATATATG+TGG	-	chr1_3:19725991-19726010	Msa0108350:CDS	30.0%
GATACAATGAAAATCTTGTG+GGG	+	chr1_3:19726279-19726298	None:intergenic	30.0%
TTGTATCATTAGCAAGCAAA+TGG	-	chr1_3:19726292-19726311	Msa0108350:intron	30.0%
TTGGTATATTGATATCTGTG+TGG	-	chr1_3:19726315-19726334	Msa0108350:intron	30.0%
TGGTATATTGATATCTGTGT+GGG	-	chr1_3:19726316-19726335	Msa0108350:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	19725156	19728023	19725156	ID=Msa0108350;Name=Msa0108350
chr1_3	mRNA	19725156	19728023	19725156	ID=Msa0108350-mRNA-1;Parent=Msa0108350;Name=Msa0108350-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0\|0\|0\|1\|0.83\|0.57\|7\|0\|293
chr1_3	exon	19728003	19728023	19728003	ID=Msa0108350-mRNA-1:exon:7121;Parent=Msa0108350-mRNA-1
chr1_3	exon	19727709	19727881	19727709	ID=Msa0108350-mRNA-1:exon:7120;Parent=Msa0108350-mRNA-1
chr1_3	exon	19727449	19727556	19727449	ID=Msa0108350-mRNA-1:exon:7119;Parent=Msa0108350-mRNA-1
chr1_3	exon	19726915	19727121	19726915	ID=Msa0108350-mRNA-1:exon:7118;Parent=Msa0108350-mRNA-1
chr1_3	exon	19726758	19726875	19726758	ID=Msa0108350-mRNA-1:exon:7117;Parent=Msa0108350-mRNA-1
chr1_3	exon	19725965	19726149	19725965	ID=Msa0108350-mRNA-1:exon:7116;Parent=Msa0108350-mRNA-1
chr1_3	exon	19725156	19725222	19725156	ID=Msa0108350-mRNA-1:exon:7115;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19728003	19728023	19728003	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19727709	19727881	19727709	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19727449	19727556	19727449	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19726915	19727121	19726915	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19726758	19726875	19726758	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19725965	19726149	19725965	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1
chr1_3	CDS	19725156	19725222	19725156	ID=Msa0108350-mRNA-1:cds;Parent=Msa0108350-mRNA-1

Gene Sequence

>Msa0108350

ATGACTAGAAAACTAGCAAAAGTTATGTGGCCTGCATTAGTGGCTAACAAGATACTAAACAGGAAACTTGGAAGCAGAAATTTCATAGCAGATTATCCAAGTTCTACAGATCCTTTATTGGCTACCAATAATGATGATCAATTATCTCTAAGCACCAAATCCATTAATGATCATAGTGATACACAAAAGTACAAGGTTTTTGTTAGTACATGGAACATAGGTGGAATTGCACCAGATGAAGGGTTGAATATTGAGGATTTGTTAGAGACATGCAGCAAGTCCTTTGATATCTATGTATTTGGGTTTCAAGAAATTGTGCCACTAAATGCATCAAATGTATTGGGATCAGAAGATAGTAAGATTTCAACAAAATGGAATTCTTTAATTAGAAATGCTTTGAACAAGAGGACACATCACTATTGTTGCAAAGATATTGAAAGGGATGATGATGATGATAATATAGAGCAAGATTTGAAGAATATTTGTCAAGGAAATATTCCAGCTCAGCACAAGCAAATGGTTGGTATATTGATATCTGTGTGGGTTAGAAGTGACCTTAGTCCTTTCATTAGACATCCATGTGTCTCATGTGTTGGATGTGGCATCATGGGCTTCTTAGGCAACAAGGGTTCTGTATCTGTGAGATTTCTATTACATGAAACAAGCTTCTGCTTTGTGTGCAGTCATCTAGCTTCTGGAGGAAAAGAAGGAGATGAGAAGCATAGGAATTCTAATGTTGCTGAAATATTTTCTAGGACAACTTTTCCTAAAGGCACTATTTTGAATTTACCAAGAAAGATTCTTGATCATGATCATGTAATATTGCTTGGAGATTTAAATTACAGAATATCTCTTCCGGAAGAAACCACACGCTTACTT

Protein sequence

>Msa0108350

MTRKLAKVMWPALVANKILNRKLGSRNFIADYPSSTDPLLATNNDDQLSLSTKSINDHSDTQKYKVFVSTWNIGGIAPDEGLNIEDLLETCSKSFDIYVFGFQEIVPLNASNVLGSEDSKISTKWNSLIRNALNKRTHHYCCKDIERDDDDDNIEQDLKNICQGNIPAQHKQMVGILISVWVRSDLSPFIRHPCVSCVGCGIMGFLGNKGSVSVRFLLHETSFCFVCSHLASGGKEGDEKHRNSNVAEIFSRTTFPKGTILNLPRKILDHDHVILLGDLNYRISLPEETTRLL