AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1434100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1434100 MtrunA17_Chr7g0263071 99.537 216 1 0 1 216 1 216 5.35e-161 443
Msa1434100 MtrunA17_Chr1g0182321 94.444 216 12 0 1 216 1 216 6.22e-156 430
Msa1434100 MtrunA17_Chr1g0182301 93.981 216 13 0 1 216 1 216 3.84e-155 428
Msa1434100 MtrunA17_Chr1g0212521 94.634 205 11 0 11 215 11 215 4.29e-144 400
Msa1434100 MtrunA17_Chr4g0030911 94.634 205 10 1 11 215 11 214 5.21e-143 397
Msa1434100 MtrunA17_Chr2g0327911 77.315 216 45 3 1 215 1 213 6.59e-117 331
Msa1434100 MtrunA17_Chr7g0250531 60.577 208 71 3 11 216 4 202 1.28e-86 254
Msa1434100 MtrunA17_Chr4g0055651 58.654 208 75 4 11 216 4 202 3.98e-85 250
Msa1434100 MtrunA17_Chr8g0335471 59.223 206 78 2 11 216 4 203 1.16e-83 247
Msa1434100 MtrunA17_Chr2g0276931 55.288 208 81 4 11 215 5 203 4.00e-79 235
Msa1434100 MtrunA17_Chr6g0458081 47.368 209 103 4 12 214 3 210 2.16e-60 188
Msa1434100 MtrunA17_Chr2g0290891 46.890 209 103 3 12 213 3 210 1.65e-59 186
Msa1434100 MtrunA17_Chr3g0112681 45.327 214 104 4 11 216 8 216 2.71e-59 185
Msa1434100 MtrunA17_Chr4g0032201 42.991 214 116 3 7 216 4 215 4.37e-59 185
Msa1434100 MtrunA17_Chr2g0283131 44.037 218 109 3 7 216 5 217 8.51e-57 179
Msa1434100 MtrunA17_Chr2g0276941 43.128 211 114 2 11 216 11 220 1.10e-56 179
Msa1434100 MtrunA17_Chr4g0046261 44.495 218 108 5 7 216 5 217 2.88e-56 177
Msa1434100 MtrunA17_Chr4g0040781 44.954 218 107 5 7 216 5 217 9.20e-56 176
Msa1434100 MtrunA17_Chr2g0315551 43.458 214 116 2 7 216 5 217 2.03e-55 176
Msa1434100 MtrunA17_Chr1g0189361 42.453 212 111 2 13 214 12 222 3.09e-55 175
Msa1434100 MtrunA17_Chr7g0265001 45.673 208 101 5 12 214 13 213 6.41e-55 174
Msa1434100 MtrunA17_Chr8g0349711 43.750 208 109 4 14 214 5 211 1.33e-54 173
Msa1434100 MtrunA17_Chr1g0180291 48.792 207 95 7 12 214 11 210 2.51e-54 172
Msa1434100 MtrunA17_Chr3g0123821 42.791 215 103 5 13 214 14 221 3.50e-54 172
Msa1434100 MtrunA17_Chr8g0381841 43.056 216 106 4 13 216 15 225 1.49e-53 171
Msa1434100 MtrunA17_Chr1g0159481 45.933 209 100 7 11 214 13 213 2.21e-53 170
Msa1434100 MtrunA17_Chr3g0130271 44.498 209 103 6 11 214 8 208 3.04e-53 170
Msa1434100 MtrunA17_Chr8g0384311 43.925 214 106 7 6 214 4 208 3.91e-53 169
Msa1434100 MtrunA17_Chr2g0319951 49.123 171 86 1 7 177 5 174 5.22e-53 169
Msa1434100 MtrunA17_Chr8g0349681 42.995 207 115 2 9 213 378 583 1.39e-52 177
Msa1434100 MtrunA17_Chr8g0349681 45.763 177 91 2 12 184 172 347 5.63e-48 165
Msa1434100 MtrunA17_Chr8g0349681 49.045 157 79 1 14 170 12 167 5.62e-46 160
Msa1434100 MtrunA17_Chr7g0234201 42.718 206 110 5 14 214 12 214 3.09e-52 167
Msa1434100 MtrunA17_Chr1g0174281 41.589 214 111 4 13 215 13 223 3.19e-52 167
Msa1434100 MtrunA17_Chr2g0325091 42.925 212 111 3 11 216 7 214 1.10e-51 166
Msa1434100 MtrunA17_Chr1g0197331 42.857 217 115 6 1 214 1 211 2.95e-51 165
Msa1434100 MtrunA17_Chr8g0346421 46.667 165 87 1 13 177 24 187 6.96e-51 164
Msa1434100 MtrunA17_Chr8g0351861 42.647 204 113 2 13 213 14 216 1.45e-50 164
Msa1434100 MtrunA17_Chr7g0214241 39.130 207 119 4 14 214 14 219 1.11e-49 161
Msa1434100 MtrunA17_Chr6g0482721 41.262 206 116 2 13 214 10 214 1.38e-49 160
Msa1434100 MtrunA17_Chr7g0275991 42.775 173 98 1 7 179 12 183 6.26e-49 159
Msa1434100 MtrunA17_Chr8g0392931 44.076 211 104 4 13 214 12 217 1.65e-47 155
Msa1434100 MtrunA17_Chr7g0231131 45.455 165 89 1 13 177 13 176 2.03e-47 155
Msa1434100 MtrunA17_Chr3g0109721 38.191 199 111 4 17 214 12 199 4.91e-45 149
Msa1434100 MtrunA17_Chr1g0199331 44.242 165 90 2 16 179 32 195 1.24e-44 147
Msa1434100 MtrunA17_Chr8g0390561 43.478 161 90 1 17 177 11 170 1.94e-44 147
Msa1434100 MtrunA17_Chr5g0433561 36.869 198 115 3 17 214 12 199 5.32e-44 146
Msa1434100 MtrunA17_Chr8g0382221 38.191 199 110 3 17 205 11 206 3.88e-42 141
Msa1434100 MtrunA17_Chr4g0034871 42.135 178 93 5 5 176 2 175 2.26e-40 137
Msa1434100 MtrunA17_Chr5g0400131 36.364 209 127 3 5 213 3 205 3.74e-40 136
Msa1434100 MtrunA17_Chr3g0123971 37.186 199 118 3 17 213 11 204 2.98e-39 134
Msa1434100 MtrunA17_Chr4g0055081 40.000 170 97 2 5 174 3 167 1.42e-38 132
Msa1434100 MtrunA17_Chr2g0331691 36.408 206 117 5 15 214 10 207 1.60e-36 127
Msa1434100 MtrunA17_Chr6g0466301 40.120 167 94 3 15 175 8 174 3.00e-36 126
Msa1434100 MtrunA17_Chr6g0465491 40.120 167 94 3 15 175 8 174 3.00e-36 126
Msa1434100 MtrunA17_Chr8g0383261 37.853 177 104 2 15 185 12 188 6.21e-36 125
Msa1434100 MtrunA17_Chr4g0004661 36.893 206 116 5 15 214 64 261 7.99e-36 127
Msa1434100 MtrunA17_Chr1g0201121 35.176 199 121 3 15 205 8 206 2.77e-35 124
Msa1434100 MtrunA17_Chr6g0466161 34.444 180 112 3 32 205 7 186 8.84e-27 102
Msa1434100 MtrunA17_Chr6g0465471 33.889 180 113 3 32 205 7 186 4.58e-26 100
Msa1434100 MtrunA17_Chr1g0178291 41.358 162 87 3 18 179 431 584 1.35e-25 103
Msa1434100 MtrunA17_Chr2g0325101 32.934 167 99 5 15 170 6 170 1.73e-25 98.2
Msa1434100 MtrunA17_Chr4g0037021 32.979 188 113 6 15 191 6 191 2.39e-25 97.8
Msa1434100 MtrunA17_Chr3g0117931 32.335 167 100 5 15 170 8 172 1.32e-24 96.3
Msa1434100 MtrunA17_Chr3g0138121 28.351 194 130 5 3 192 2 190 2.17e-24 96.3
Msa1434100 MtrunA17_Chr3g0138101 28.351 194 130 5 3 192 2 190 2.54e-24 95.9
Msa1434100 MtrunA17_Chr3g0138111 28.351 194 130 5 3 192 2 190 2.92e-24 95.9
Msa1434100 MtrunA17_Chr8g0371501 32.335 167 100 6 15 170 6 170 3.33e-23 92.4
Msa1434100 MtrunA17_Chr6g0486041 31.138 167 102 5 15 170 6 170 5.91e-23 92.0
Msa1434100 MtrunA17_Chr5g0405791 32.335 167 100 6 15 170 6 170 6.90e-23 91.7
Msa1434100 MtrunA17_Chr4g0046211 34.677 124 78 3 15 137 6 127 2.76e-22 88.6
Msa1434100 MtrunA17_Chr6g0466431 35.789 95 52 1 15 109 8 93 5.46e-17 73.6
Msa1434100 MtrunA17_Chr6g0465611 35.789 95 52 1 15 109 8 93 5.46e-17 73.6
Msa1434100 MtrunA17_Chr5g0443631 25.466 161 111 3 16 170 105 262 8.45e-13 65.9
Msa1434100 MtrunA17_Chr5g0434561 31.707 164 93 6 16 170 20 173 6.46e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1434100 AT3G53610.2 93.689 206 13 0 11 216 11 216 2.69e-145 404
Msa1434100 AT3G53610.3 93.689 206 13 0 11 216 11 216 2.69e-145 404
Msa1434100 AT3G53610.1 93.689 206 13 0 11 216 11 216 2.69e-145 404
Msa1434100 AT5G59840.1 94.660 206 11 0 11 216 11 216 5.31e-145 403
Msa1434100 AT3G46060.2 93.689 206 13 0 11 216 11 216 1.51e-144 402
Msa1434100 AT3G46060.3 93.689 206 13 0 11 216 11 216 1.51e-144 402
Msa1434100 AT3G46060.1 93.689 206 13 0 11 216 11 216 1.51e-144 402
Msa1434100 AT5G03520.1 86.916 214 27 1 1 214 1 213 1.51e-130 366
Msa1434100 AT3G09900.1 85.116 215 31 1 1 214 1 215 5.43e-128 360
Msa1434100 AT5G03520.2 86.061 165 22 1 50 214 40 203 1.95e-103 297
Msa1434100 AT1G02130.1 60.194 206 76 2 11 216 4 203 1.28e-87 257
Msa1434100 AT4G17530.1 58.654 208 75 4 11 216 4 202 7.06e-83 245
Msa1434100 AT5G47200.1 58.173 208 76 4 11 216 4 202 3.68e-82 243
Msa1434100 AT3G11730.1 56.459 209 79 4 11 215 4 204 8.06e-80 238
Msa1434100 AT4G17160.1 48.544 206 101 3 12 215 3 205 1.59e-65 201
Msa1434100 AT4G35860.1 48.571 210 101 4 12 215 3 211 6.30e-64 197
Msa1434100 AT4G17170.1 47.143 210 104 4 12 215 3 211 2.03e-61 191
Msa1434100 AT1G09630.1 44.860 214 113 2 7 216 4 216 1.42e-60 189
Msa1434100 AT3G46830.1 43.636 220 108 3 7 216 4 217 1.68e-57 181
Msa1434100 AT3G46830.2 43.636 220 108 3 7 216 4 217 1.68e-57 181
Msa1434100 AT5G59150.1 49.123 171 86 1 7 177 4 173 5.09e-57 180
Msa1434100 AT1G07410.1 42.009 219 110 4 7 216 4 214 5.29e-57 180
Msa1434100 AT4G18430.1 44.444 216 107 3 7 214 5 215 6.46e-57 180
Msa1434100 AT1G16920.1 45.581 215 105 4 7 214 5 214 6.55e-57 180
Msa1434100 AT3G07410.1 42.995 207 112 3 13 214 10 215 5.21e-56 177
Msa1434100 AT5G60860.1 44.495 218 108 5 7 216 5 217 1.96e-55 176
Msa1434100 AT4G39990.1 44.340 212 107 4 13 216 15 223 4.70e-55 175
Msa1434100 AT5G45750.1 44.340 212 112 3 7 214 5 214 5.12e-55 175
Msa1434100 AT3G15060.1 45.413 218 106 5 7 216 5 217 5.88e-55 175
Msa1434100 AT2G33870.1 43.379 219 110 4 7 216 5 218 9.06e-55 174
Msa1434100 AT4G18800.1 43.192 213 115 3 7 216 5 214 2.74e-54 173
Msa1434100 AT5G47520.1 43.458 214 107 3 13 216 12 221 3.28e-54 173
Msa1434100 AT2G43130.1 41.176 204 117 2 13 214 10 212 1.35e-53 171
Msa1434100 AT1G05810.1 41.905 210 114 3 13 216 10 217 1.45e-53 171
Msa1434100 AT1G28550.1 42.922 219 111 4 7 216 5 218 1.59e-53 171
Msa1434100 AT1G05810.2 40.670 209 118 2 13 216 56 263 4.97e-53 171
Msa1434100 AT1G01200.1 47.337 169 89 0 13 181 26 194 1.29e-52 169
Msa1434100 AT1G06400.1 43.256 215 110 4 7 214 5 214 1.36e-52 169
Msa1434100 AT2G31680.1 47.879 165 85 1 13 177 10 173 3.74e-52 167
Msa1434100 AT1G43890.3 50.898 167 78 4 6 170 4 168 8.25e-52 166
Msa1434100 AT1G43890.1 50.898 167 78 4 6 170 4 168 8.25e-52 166
Msa1434100 AT1G43890.2 50.898 167 78 4 6 170 4 168 8.25e-52 166
Msa1434100 AT5G47960.1 40.092 217 110 3 13 216 13 222 1.03e-51 167
Msa1434100 AT5G03530.2 45.283 212 103 7 8 214 6 209 2.40e-51 165
Msa1434100 AT5G03530.1 45.283 212 103 7 8 214 6 209 2.40e-51 165
Msa1434100 AT3G12160.1 40.376 213 112 4 13 215 13 220 3.54e-51 165
Msa1434100 AT5G65270.1 40.376 213 117 4 13 216 15 226 4.69e-51 165
Msa1434100 AT1G73640.1 45.596 193 100 2 13 201 11 202 1.61e-50 164
Msa1434100 AT1G18200.1 50.296 169 83 1 13 181 11 178 2.14e-50 164
Msa1434100 AT3G54840.1 44.382 178 94 3 3 179 24 197 2.33e-47 155
Msa1434100 AT3G54840.2 46.154 169 86 3 3 170 24 188 6.84e-47 153
Msa1434100 AT3G54840.3 45.399 163 87 2 18 179 2 163 3.02e-46 151
Msa1434100 AT3G09910.5 46.486 185 95 4 8 190 6 188 2.88e-44 147
Msa1434100 AT3G09910.4 46.486 185 95 4 8 190 6 188 2.88e-44 147
Msa1434100 AT4G35860.2 46.667 165 81 4 57 215 2 165 3.81e-44 145
Msa1434100 AT4G19640.1 38.191 199 113 4 17 215 12 200 1.03e-42 143
Msa1434100 AT3G09910.3 47.126 174 88 4 8 179 6 177 5.63e-42 141
Msa1434100 AT3G09910.1 47.126 174 88 4 8 179 6 177 5.63e-42 141
Msa1434100 AT3G09910.2 47.126 174 88 4 8 179 6 177 5.63e-42 141
Msa1434100 AT3G18820.1 42.135 178 93 4 5 176 2 175 4.82e-41 139
Msa1434100 AT4G39890.1 43.396 159 88 2 17 174 11 168 8.06e-41 139
Msa1434100 AT2G22290.1 36.869 198 122 2 17 214 11 205 9.99e-41 138
Msa1434100 AT5G45130.1 36.683 199 116 5 17 215 12 200 1.95e-40 137
Msa1434100 AT2G44610.1 36.493 211 128 3 5 215 3 207 2.93e-40 137
Msa1434100 AT5G10260.1 36.364 198 124 2 17 214 11 206 1.86e-39 135
Msa1434100 AT5G45130.2 36.041 197 116 5 19 215 22 208 1.29e-38 132
Msa1434100 AT5G64990.1 37.374 198 121 3 17 213 9 204 2.96e-38 132
Msa1434100 AT1G52280.1 37.864 206 113 5 15 214 8 204 5.53e-38 131
Msa1434100 AT5G59150.2 48.760 121 61 1 57 177 3 122 1.37e-37 129
Msa1434100 AT4G09720.1 38.191 199 114 4 15 205 8 205 1.55e-37 130
Msa1434100 AT1G22740.1 38.071 197 114 4 15 205 8 202 2.73e-37 129
Msa1434100 AT1G49300.2 40.000 175 95 4 5 173 2 172 2.84e-37 129
Msa1434100 AT1G49300.1 40.000 175 95 4 5 173 2 172 2.84e-37 129
Msa1434100 AT3G16100.1 37.864 206 113 4 15 214 8 204 1.14e-36 127
Msa1434100 AT2G21880.1 36.058 208 111 4 15 205 9 211 7.09e-36 125
Msa1434100 AT4G09720.4 37.745 204 113 5 15 205 8 210 1.08e-35 125
Msa1434100 AT5G64990.2 36.098 205 121 4 17 213 9 211 1.39e-35 125
Msa1434100 AT4G09720.3 35.238 210 116 4 15 205 8 216 2.21e-34 122
Msa1434100 AT5G39620.1 38.462 169 92 3 16 175 6 171 9.75e-34 120
Msa1434100 AT4G19640.2 38.843 121 74 0 17 137 12 132 7.86e-30 108
Msa1434100 AT4G19640.3 38.843 121 74 0 17 137 12 132 5.12e-29 107
Msa1434100 AT4G09720.6 38.235 170 96 4 44 205 3 171 1.81e-28 105
Msa1434100 AT4G09720.5 38.235 170 96 4 44 205 3 171 1.81e-28 105
Msa1434100 AT4G09720.2 38.235 170 96 4 44 205 3 171 1.81e-28 105
Msa1434100 AT2G21880.4 35.519 183 96 4 40 205 9 186 8.12e-28 104
Msa1434100 AT2G21880.3 35.519 183 96 4 40 205 9 186 8.12e-28 104
Msa1434100 AT4G35020.1 35.928 167 94 6 15 170 6 170 6.75e-27 102
Msa1434100 AT4G35020.2 35.928 167 94 6 15 170 6 170 6.75e-27 102
Msa1434100 AT4G35020.3 35.928 167 94 6 15 170 6 170 6.75e-27 102
Msa1434100 AT2G21880.2 32.212 208 111 5 15 205 9 203 5.02e-26 100
Msa1434100 AT2G44690.1 32.335 167 100 5 15 170 18 182 8.90e-26 99.8
Msa1434100 AT5G20010.1 28.972 214 142 6 3 212 2 209 1.25e-25 99.8
Msa1434100 AT3G48040.1 32.934 167 99 5 15 170 8 172 1.25e-25 99.4
Msa1434100 AT5G45970.1 31.138 167 102 5 15 170 6 170 3.70e-25 97.8
Msa1434100 AT5G62880.1 31.844 179 109 5 15 182 8 184 3.80e-25 98.2
Msa1434100 AT5G55080.1 32.716 162 105 3 16 177 14 171 8.10e-25 97.8
Msa1434100 AT3G51300.1 32.335 167 100 6 15 170 6 170 1.14e-24 96.7
Msa1434100 AT1G20090.1 34.132 167 97 6 15 170 5 169 1.36e-24 96.3
Msa1434100 AT5G55190.1 28.037 214 142 6 3 209 2 210 1.48e-24 97.1
Msa1434100 AT1G75840.1 33.533 167 98 6 15 170 6 170 1.80e-24 95.9
Msa1434100 AT5G20020.1 30.286 175 117 4 3 177 2 171 4.92e-24 95.5
Msa1434100 AT2G17800.2 32.934 167 99 6 15 170 6 170 5.36e-24 94.7
Msa1434100 AT2G17800.1 32.934 167 99 6 15 170 6 170 5.36e-24 94.7
Msa1434100 AT4G28950.1 31.515 165 102 5 15 170 6 168 1.73e-23 94.0
Msa1434100 AT4G35950.1 32.335 167 100 6 15 170 6 170 4.50e-23 92.4
Msa1434100 AT3G48040.2 32.075 159 95 5 15 162 8 164 5.46e-23 91.7
Msa1434100 AT5G62880.2 32.075 159 95 5 15 162 8 164 5.50e-22 89.4
Msa1434100 AT4G35020.4 36.207 116 71 3 15 129 6 119 7.67e-19 80.1
Msa1434100 AT4G35020.5 36.207 116 71 3 15 129 6 119 7.67e-19 80.1
Msa1434100 AT5G46025.1 35.294 102 64 2 77 177 6 106 1.63e-15 70.1
Msa1434100 AT3G51290.2 30.769 143 86 6 44 175 636 776 7.56e-13 67.4
Msa1434100 AT5G37680.1 27.215 158 108 3 16 170 20 173 4.12e-12 62.8
Msa1434100 AT2G15310.1 28.058 139 88 6 16 153 18 145 1.33e-11 62.0

Find 91 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCACTCAGATTCAGTTATT+TGG 0.114215 tig0027465:+26818 None:intergenic
GTTGGTTAATCTTGATTATC+TGG 0.131110 tig0027465:+27190 None:intergenic
CACGGTGTGGTTAACATTTA+TGG 0.143277 tig0027465:-26846 Msa1434100:three_prime_UTR
CTCATTAAACTTCTCCTAAT+TGG 0.159282 tig0027465:-29276 Msa1434100:CDS
GTTCCTGCTTTAAGCCTTAT+TGG 0.168990 tig0027465:+26891 None:intergenic
AGCGAATCAAATTGCAAATA+TGG 0.180362 tig0027465:-28915 Msa1434100:CDS
CACACATTCATTCTTGAGTT+TGG 0.211920 tig0027465:-26763 Msa1434100:three_prime_UTR
TTAGGCTGTGCCAACTTCTA+AGG 0.253740 tig0027465:-27649 Msa1434100:intron
CTGCATGTTGTGGTTCTTAA+TGG 0.257090 tig0027465:-27128 Msa1434100:exon
TCAGCATTGATTTCAAAATA+AGG 0.261517 tig0027465:-28957 Msa1434100:intron
TTCCTTTCTTGAAGCCAATA+AGG 0.303057 tig0027465:-26905 Msa1434100:three_prime_UTR
TCACTAGTTGAGCGTTGAAT+TGG 0.317922 tig0027465:+29601 None:intergenic
TGACAATGTTAACAAGATAT+TGG 0.321340 tig0027465:-27812 Msa1434100:CDS
TCACTCAGATTCAGTTATTT+GGG 0.335202 tig0027465:+26819 None:intergenic
TCATCTCATGTCCAGATATC+AGG 0.336735 tig0027465:-27867 Msa1434100:intron
TGGTCAAGGAACTTATGATA+TGG 0.358979 tig0027465:-26629 Msa1434100:three_prime_UTR
CAAGCGCTTGCAGATGAATA+TGG 0.360715 tig0027465:-27624 Msa1434100:CDS
TTGGTTAATCTTGATTATCT+GGG 0.363321 tig0027465:+27191 None:intergenic
CACTAGTTGAGCGTTGAATT+GGG 0.367061 tig0027465:+29602 None:intergenic
CCTGGGCAGCCCCTGCTCCC+TGG 0.373973 tig0027465:+27163 None:intergenic
ACCACACCATACTTGGCTTA+TGG 0.376966 tig0027465:-26870 Msa1434100:three_prime_UTR
TGGTACCGCTATCACCAATT+AGG 0.378492 tig0027465:+29262 None:intergenic
AGCAGGAACCACACCATACT+TGG 0.381808 tig0027465:-26877 Msa1434100:three_prime_UTR
GCAGCCCCTGCTCCCTGGTC+TGG 0.385231 tig0027465:+27168 None:intergenic
AGGAAAGTTAAGATCTAAAC+AGG 0.396767 tig0027465:+29520 None:intergenic
ATAAGGACAATTGAGCTTGA+TGG 0.403140 tig0027465:-28940 Msa1434100:CDS
CCAGGGAGCAGGGGCTGCCC+AGG 0.406408 tig0027465:-27163 Msa1434100:CDS
CATGTCCAGATATCAGGAAT+TGG 0.411917 tig0027465:-27861 Msa1434100:intron
TCGGACACTCGCAGAGGAAT+AGG 0.419201 tig0027465:-29355 Msa1434100:intron
GCGAATCAAATTGCAAATAT+GGG 0.419547 tig0027465:-28914 Msa1434100:CDS
AGCCTTATTGGCTTCAAGAA+AGG 0.428738 tig0027465:+26903 None:intergenic
CCGTGACCATAAGCCAAGTA+TGG 0.430523 tig0027465:+26864 None:intergenic
ATCGTAATCAGCACGAGCTC+TGG 0.430906 tig0027465:+29302 None:intergenic
CATAATTAATTTCCCAACAT+AGG 0.431227 tig0027465:+26984 None:intergenic
CCCCTGCTCCCTGGTCTGGT+TGG 0.433352 tig0027465:+27172 None:intergenic
CGATTTCGAACTATCACAAC+TGG 0.433779 tig0027465:-28874 Msa1434100:intron
AGAGAAAAGGAAAGAAAAGA+GGG 0.437595 tig0027465:+29555 None:intergenic
GAGAGAAAAGGAAAGAAAAG+AGG 0.440554 tig0027465:+29554 None:intergenic
AGAATGAATGTGTGGATGTC+TGG 0.447349 tig0027465:+26772 None:intergenic
TGCATGTTGTGGTTCTTAAT+GGG 0.450982 tig0027465:-27127 Msa1434100:exon
GCCTATGTTTATTTCTGCTG+TGG 0.464643 tig0027465:-26689 Msa1434100:three_prime_UTR
GAAAACGAGAAAGAGAGAAA+AGG 0.470733 tig0027465:+29542 None:intergenic
GTTCTTAATGGGAAGCTTGA+GGG 0.477784 tig0027465:-27116 Msa1434100:exon
TTTACTTGTATATGACGTTA+CGG 0.479177 tig0027465:-28110 Msa1434100:CDS
TCATATCATATTAATTAAAA+CGG 0.479699 tig0027465:+26580 None:intergenic
ATAATTAATTTCCCAACATA+GGG 0.480029 tig0027465:+26985 None:intergenic
TTCCTCTTCCTCATCTTCAC+AGG 0.480337 tig0027465:-29502 Msa1434100:intron
AGTTCGAAATCGCTCTTGAC+CGG 0.481009 tig0027465:+28885 None:intergenic
AACCAACCAGACCAGGGAGC+AGG 0.489253 tig0027465:-27174 Msa1434100:CDS
TGCTACTCATTGGGTTGGAA+TGG 0.494378 tig0027465:-27016 Msa1434100:three_prime_UTR
CAAGATTAACCAACCAGACC+AGG 0.502790 tig0027465:-27181 Msa1434100:CDS
ACAGTTGCTACTCATTGGGT+TGG 0.505420 tig0027465:-27021 Msa1434100:three_prime_UTR
CATATTCATCTGCAAGCGCT+TGG 0.507439 tig0027465:+27625 None:intergenic
TACCTGTGAAGATGAGGAAG+AGG 0.514002 tig0027465:+29500 None:intergenic
AGCGCTTGGCCCTTAGAAGT+TGG 0.514221 tig0027465:+27639 None:intergenic
AGCTTACTACCGTGGAGCCA+TGG 0.521985 tig0027465:-28137 Msa1434100:CDS
TCTTGAGTTTGGAGTTTCAA+AGG 0.522996 tig0027465:-26752 Msa1434100:three_prime_UTR
TAGGCTGTGCCAACTTCTAA+GGG 0.524551 tig0027465:-27648 Msa1434100:CDS
AGAATGAAAAGAAGAATCAA+AGG 0.526724 tig0027465:-29662 Msa1434100:five_prime_UTR
AACAAATCTGAATGTGGAGG+AGG 0.528959 tig0027465:-27486 Msa1434100:CDS
CTGACATGGATGAAAGCAAA+CGG 0.531518 tig0027465:-27777 Msa1434100:CDS
GCTTACTACCGTGGAGCCAT+GGG 0.548016 tig0027465:-28136 Msa1434100:CDS
TGGAAATACCTGTGAAGATG+AGG 0.548935 tig0027465:+29494 None:intergenic
TGACATGGATGAAAGCAAAC+GGG 0.551245 tig0027465:-27776 Msa1434100:intron
AATGTTAACAAGATATTGGT+GGG 0.552268 tig0027465:-27808 Msa1434100:CDS
TAATCAGCACGAGCTCTGGC+AGG 0.555758 tig0027465:+29306 None:intergenic
CCATACTTGGCTTATGGTCA+CGG 0.557303 tig0027465:-26864 Msa1434100:three_prime_UTR
ACCAACCAGACCAGGGAGCA+GGG 0.559075 tig0027465:-27173 Msa1434100:CDS
GGTTCTTAATGGGAAGCTTG+AGG 0.564712 tig0027465:-27117 Msa1434100:exon
CAATGTTAACAAGATATTGG+TGG 0.567581 tig0027465:-27809 Msa1434100:CDS
TGGTTAATCTTGATTATCTG+GGG 0.569962 tig0027465:+27192 None:intergenic
ACTAGTTGAGCGTTGAATTG+GGG 0.584990 tig0027465:+29603 None:intergenic
GAATGAAAAGAAGAATCAAA+GGG 0.587691 tig0027465:-29661 Msa1434100:five_prime_UTR
AGTTTGGAGTTTCAAAGGCG+TGG 0.595003 tig0027465:-26747 Msa1434100:three_prime_UTR
TCAGCACGAGCTCTGGCAGG+TGG 0.599524 tig0027465:+29309 None:intergenic
GTAAAATACCCATGGCTCCA+CGG 0.601071 tig0027465:+28128 None:intergenic
TTGCAAATATGGGACACAGC+CGG 0.602143 tig0027465:-28904 Msa1434100:CDS
TGCTAAAACAAATCTGAATG+TGG 0.604329 tig0027465:-27492 Msa1434100:CDS
AAGCCAATAAGGCTTAAAGC+AGG 0.605417 tig0027465:-26894 Msa1434100:three_prime_UTR
CTTCTCCTAATTGGTGATAG+CGG 0.620219 tig0027465:-29267 Msa1434100:intron
CCAACCAGACCAGGGAGCAG+GGG 0.621818 tig0027465:-27172 Msa1434100:CDS
GGTGGGCAACAAAGCTGACA+TGG 0.624736 tig0027465:-27791 Msa1434100:CDS
AGATACTGACTCAAAATCTG+AGG 0.634590 tig0027465:-27423 Msa1434100:intron
ATATACAAGTAAAATACCCA+TGG 0.634609 tig0027465:+28120 None:intergenic
TAAAACAAATCTGAATGTGG+AGG 0.641771 tig0027465:-27489 Msa1434100:CDS
ACCACAGCAGAAATAAACAT+AGG 0.642974 tig0027465:+26688 None:intergenic
ACCATAAGCCAAGTATGGTG+TGG 0.653928 tig0027465:+26869 None:intergenic
CAAACTCAAGAATGAATGTG+TGG 0.658578 tig0027465:+26764 None:intergenic
CTTGGCTTATGGTCACGGTG+TGG 0.658669 tig0027465:-26859 Msa1434100:three_prime_UTR
AAGATTAACCAACCAGACCA+GGG 0.666777 tig0027465:-27180 Msa1434100:CDS
GTGGATGTCTGGCATCAATG+TGG 0.721252 tig0027465:+26783 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATATCATATTAATTAAAA+CGG + tig0027465:29656-29675 None:intergenic 10.0%
!! GATTGTAATTAATTAATGAT+CGG - tig0027465:26859-26878 Msa1434100:three_prime_UTR 15.0%
!! TAATATTACAATAACAACAA+AGG + tig0027465:27966-27985 None:intergenic 15.0%
!! ATTTACATATACAAAATCAT+AGG + tig0027465:28039-28058 None:intergenic 15.0%
!! AAAGATGCAATTATAAAAAA+GGG + tig0027465:28896-28915 None:intergenic 15.0%
!! CCAAACATAGAAAATAAAAA+TGG + tig0027465:26994-27013 None:intergenic 20.0%
!!! CCATTTTTATTTTCTATGTT+TGG - tig0027465:26991-27010 Msa1434100:three_prime_UTR 20.0%
!!! TTATTTTCTATGTTTGGTAA+TGG - tig0027465:26997-27016 Msa1434100:three_prime_UTR 20.0%
!!! GATTATGCTATTTTTCATAT+TGG - tig0027465:27094-27113 Msa1434100:three_prime_UTR 20.0%
!! GAATTTAAGGATAATAAAAG+AGG + tig0027465:28209-28228 None:intergenic 20.0%
!! TACGAAGATATTACAATTTA+AGG + tig0027465:28238-28257 None:intergenic 20.0%
!!! TTTTATGTACTTATATGCAT+TGG - tig0027465:28518-28537 Msa1434100:intron 20.0%
!! TTTATGTACTTATATGCATT+GGG - tig0027465:28519-28538 Msa1434100:intron 20.0%
!! AATCAATGATAACTAGTAAA+AGG + tig0027465:28848-28867 None:intergenic 20.0%
!! GGTTAAAAAAAAAAAAAGTC+GGG + tig0027465:28875-28894 None:intergenic 20.0%
!! GGGTTAAAAAAAAAAAAAGT+CGG + tig0027465:28876-28895 None:intergenic 20.0%
!! GAAAGATGCAATTATAAAAA+AGG + tig0027465:28897-28916 None:intergenic 20.0%
!!! TTTTATAATTGCATCTTTCA+CGG - tig0027465:28898-28917 Msa1434100:CDS 20.0%
!! ATAATTAATTTCCCAACATA+GGG + tig0027465:29251-29270 None:intergenic 20.0%
!!! AACTCTTAATTTTGTCATTT+TGG + tig0027465:29521-29540 None:intergenic 20.0%
! AGAATGAAAAGAAGAATCAA+AGG - tig0027465:26571-26590 Msa1434100:three_prime_UTR 25.0%
! GAATGAAAAGAAGAATCAAA+GGG - tig0027465:26572-26591 Msa1434100:three_prime_UTR 25.0%
! TCAATAAATGAAGAGAAAAG+TGG + tig0027465:26762-26781 None:intergenic 25.0%
! TTGTGCAATCATGTAATATT+TGG - tig0027465:27204-27223 Msa1434100:intron 25.0%
! GACATCACAAAAGAATAAAA+CGG + tig0027465:27232-27251 None:intergenic 25.0%
!!! TCAGCATTGATTTCAAAATA+AGG - tig0027465:27276-27295 Msa1434100:intron 25.0%
! AACATAGTGTAAAACATTAC+AGG + tig0027465:27429-27448 None:intergenic 25.0%
!! GTAATGTTTTACACTATGTT+TGG - tig0027465:27429-27448 Msa1434100:CDS 25.0%
! TATTAGAGAAACTTCTTTCA+TGG - tig0027465:27859-27878 Msa1434100:intron 25.0%
! ATATACAAGTAAAATACCCA+TGG + tig0027465:28116-28135 None:intergenic 25.0%
! TTTACTTGTATATGACGTTA+CGG - tig0027465:28123-28142 Msa1434100:CDS 25.0%
! TAAGGATAATAAAAGAGGAA+TGG + tig0027465:28204-28223 None:intergenic 25.0%
! TTTAAGGAAACACGAATTTA+AGG + tig0027465:28222-28241 None:intergenic 25.0%
! TAATTGTGTTCACTGTATAA+TGG + tig0027465:28267-28286 None:intergenic 25.0%
! TGACAATGTTAACAAGATAT+TGG - tig0027465:28421-28440 Msa1434100:intron 25.0%
! AATGTTAACAAGATATTGGT+GGG - tig0027465:28425-28444 Msa1434100:intron 25.0%
!!! CAAAATCTGAGGTTTTATTT+TGG - tig0027465:28821-28840 Msa1434100:intron 25.0%
!! GAGTGATTTTTGTTAAACTT+AGG + tig0027465:28964-28983 None:intergenic 25.0%
! TTGGTTAATCTTGATTATCT+GGG + tig0027465:29045-29064 None:intergenic 25.0%
! CATAATTAATTTCCCAACAT+AGG + tig0027465:29252-29271 None:intergenic 25.0%
!! AAACTGAGAAATAACTTTTG+TGG - tig0027465:29584-29603 Msa1434100:five_prime_UTR 25.0%
AGAGAAAAGGAAAGAAAAGA+GGG + tig0027465:26681-26700 None:intergenic 30.0%
AGGAAAGTTAAGATCTAAAC+AGG + tig0027465:26716-26735 None:intergenic 30.0%
!!! ATAGGTTTTGTTTGTTTCGA+TGG - tig0027465:26896-26915 Msa1434100:three_prime_UTR 30.0%
CTCATTAAACTTCTCCTAAT+TGG - tig0027465:26957-26976 Msa1434100:three_prime_UTR 30.0%
!! GTTTGATTTTGTGAAAGCTA+CGG + tig0027465:27052-27071 None:intergenic 30.0%
CAAACAAGTACTTAGTACTT+TGG - tig0027465:27067-27086 Msa1434100:three_prime_UTR 30.0%
!!! ATGCTATTTTTCATATTGGC+AGG - tig0027465:27098-27117 Msa1434100:three_prime_UTR 30.0%
! TTTTATTGCGTTTCTCTGAT+GGG - tig0027465:27138-27157 Msa1434100:CDS 30.0%
!!! ACTAGTTTTATCACAACCAT+TGG - tig0027465:27170-27189 Msa1434100:CDS 30.0%
!!! CTAGTTTTATCACAACCATT+GGG - tig0027465:27171-27190 Msa1434100:CDS 30.0%
AGCGAATCAAATTGCAAATA+TGG - tig0027465:27318-27337 Msa1434100:intron 30.0%
GCGAATCAAATTGCAAATAT+GGG - tig0027465:27319-27338 Msa1434100:intron 30.0%
!! TTCTAGCTTCACAAAAATCA+TGG + tig0027465:27498-27517 None:intergenic 30.0%
! ATTTTTGTGAAGCTAGAAGT+TGG - tig0027465:27500-27519 Msa1434100:intron 30.0%
! ACACAAATTCGTCCATTTTA+TGG - tig0027465:27536-27555 Msa1434100:intron 30.0%
! CACAAATTCGTCCATTTTAT+GGG - tig0027465:27537-27556 Msa1434100:intron 30.0%
TGTTTAACGTAAGAGAGAAT+GGG + tig0027465:28181-28200 None:intergenic 30.0%
CAATGTTAACAAGATATTGG+TGG - tig0027465:28424-28443 Msa1434100:intron 30.0%
TCATTCTCATTCATACTTGT+TGG - tig0027465:28491-28510 Msa1434100:intron 30.0%
TGCTAAAACAAATCTGAATG+TGG - tig0027465:28741-28760 Msa1434100:intron 30.0%
TAAAACAAATCTGAATGTGG+AGG - tig0027465:28744-28763 Msa1434100:intron 30.0%
! AGGTTTTCTTTTCAATAGCT+CGG - tig0027465:28767-28786 Msa1434100:intron 30.0%
TTTGTTAAACTTAGGATGCA+AGG + tig0027465:28956-28975 None:intergenic 30.0%
GTTTAACAAAAATCACTCTC+CGG - tig0027465:28966-28985 Msa1434100:intron 30.0%
TTTAACAAAAATCACTCTCC+GGG - tig0027465:28967-28986 Msa1434100:intron 30.0%
ACAAAATGGAAAACTTAGCA+TGG + tig0027465:29012-29031 None:intergenic 30.0%
TGGTTAATCTTGATTATCTG+GGG + tig0027465:29044-29063 None:intergenic 30.0%
GTTGGTTAATCTTGATTATC+TGG + tig0027465:29046-29065 None:intergenic 30.0%
! TTTTACAGTTGCTACTCATT+GGG - tig0027465:29208-29227 Msa1434100:intron 30.0%
TCACTCAGATTCAGTTATTT+GGG + tig0027465:29417-29436 None:intergenic 30.0%
! AGAAATAACTTTTGTGGTCA+AGG - tig0027465:29590-29609 Msa1434100:five_prime_UTR 30.0%
GAGAGAAAAGGAAAGAAAAG+AGG + tig0027465:26682-26701 None:intergenic 35.0%
GAAAACGAGAAAGAGAGAAA+AGG + tig0027465:26694-26713 None:intergenic 35.0%
! TTTTTCATATTGGCAGGTGT+TGG - tig0027465:27104-27123 Msa1434100:three_prime_UTR 35.0%
! CTTTTATTGCGTTTCTCTGA+TGG - tig0027465:27137-27156 Msa1434100:CDS 35.0%
! TTTTATCACAACCATTGGGT+AGG - tig0027465:27175-27194 Msa1434100:CDS 35.0%
ATAAGGACAATTGAGCTTGA+TGG - tig0027465:27293-27312 Msa1434100:intron 35.0%
ATCCAAACAAGCCCATAAAA+TGG + tig0027465:27551-27570 None:intergenic 35.0%
CGCTTATATAAGCTTGCATA+AGG - tig0027465:27614-27633 Msa1434100:CDS 35.0%
! TGGTTTGAGTTTCCATTTAG+CGG - tig0027465:27879-27898 Msa1434100:intron 35.0%
! ATGTAACTGATTCCACCTAA+TGG - tig0027465:27934-27953 Msa1434100:intron 35.0%
GTTTAACGTAAGAGAGAATG+GGG + tig0027465:28180-28199 None:intergenic 35.0%
GTGTTTAACGTAAGAGAGAA+TGG + tig0027465:28182-28201 None:intergenic 35.0%
!! AAGCATTGAAAGCCAATTGT+CGG + tig0027465:28304-28323 None:intergenic 35.0%
AAGCGCTATTACATGACAAT+AGG - tig0027465:28336-28355 Msa1434100:intron 35.0%
AGTATGAATGAGAATGAGAG+AGG + tig0027465:28488-28507 None:intergenic 35.0%
TATCCATCAATGTTCCCTTT+AGG - tig0027465:28566-28585 Msa1434100:intron 35.0%
!!! TTTGTTTTAGCACTCTGTAC+AGG + tig0027465:28733-28752 None:intergenic 35.0%
! GGTTTTCTTTTCAATAGCTC+GGG - tig0027465:28768-28787 Msa1434100:intron 35.0%
!! AGATACTGACTCAAAATCTG+AGG - tig0027465:28810-28829 Msa1434100:intron 35.0%
! ACCACAACATGCAGATTTTT+GGG + tig0027465:29099-29118 None:intergenic 35.0%
! AACCACAACATGCAGATTTT+TGG + tig0027465:29100-29119 None:intergenic 35.0%
TGCATGTTGTGGTTCTTAAT+GGG - tig0027465:29106-29125 Msa1434100:CDS 35.0%
! GTTTTACAGTTGCTACTCAT+TGG - tig0027465:29207-29226 Msa1434100:intron 35.0%
! ATGGTAGATTTTCCCTATGT+TGG - tig0027465:29236-29255 Msa1434100:intron 35.0%
! TGGTAGATTTTCCCTATGTT+GGG - tig0027465:29237-29256 Msa1434100:intron 35.0%
!! CTTAAACCCACTCTTTTTCA+GGG - tig0027465:29287-29306 Msa1434100:CDS 35.0%
!!! TTAAACCCACTCTTTTTCAG+GGG - tig0027465:29288-29307 Msa1434100:CDS 35.0%
TTCCTTTCTTGAAGCCAATA+AGG - tig0027465:29328-29347 Msa1434100:exon 35.0%
GTCACTCAGATTCAGTTATT+TGG + tig0027465:29418-29437 None:intergenic 35.0%
CAAACTCAAGAATGAATGTG+TGG + tig0027465:29472-29491 None:intergenic 35.0%
CACACATTCATTCTTGAGTT+TGG - tig0027465:29470-29489 Msa1434100:intron 35.0%
! TCTTGAGTTTGGAGTTTCAA+AGG - tig0027465:29481-29500 Msa1434100:intron 35.0%
ACCACAGCAGAAATAAACAT+AGG + tig0027465:29548-29567 None:intergenic 35.0%
TGGTCAAGGAACTTATGATA+TGG - tig0027465:29604-29623 Msa1434100:five_prime_UTR 35.0%
! GGAACATTTTCTGCATGTTT+AGG + tig0027465:29635-29654 None:intergenic 35.0%
!! ACTAGTTGAGCGTTGAATTG+GGG + tig0027465:26633-26652 None:intergenic 40.0%
!! CACTAGTTGAGCGTTGAATT+GGG + tig0027465:26634-26653 None:intergenic 40.0%
!! TCACTAGTTGAGCGTTGAAT+TGG + tig0027465:26635-26654 None:intergenic 40.0%
TGGAAATACCTGTGAAGATG+AGG + tig0027465:26742-26761 None:intergenic 40.0%
! CTTCTCCTAATTGGTGATAG+CGG - tig0027465:26966-26985 Msa1434100:three_prime_UTR 40.0%
!! GTTTGGTAATGGTATCACTC+TGG - tig0027465:27008-27027 Msa1434100:three_prime_UTR 40.0%
TTTGTGAAAGCTACGGAGTA+TGG + tig0027465:27045-27064 None:intergenic 40.0%
CACAAAAGAAACCTACCCAA+TGG + tig0027465:27189-27208 None:intergenic 40.0%
CGATTTCGAACTATCACAAC+TGG - tig0027465:27359-27378 Msa1434100:intron 40.0%
GAACATTAGGACGTGAGAAA+TGG + tig0027465:27470-27489 None:intergenic 40.0%
AATCATGGCCAGAGAACATT+AGG + tig0027465:27483-27502 None:intergenic 40.0%
!! GTCCATTTTATGGGCTTGTT+TGG - tig0027465:27546-27565 Msa1434100:intron 40.0%
GTCTAGTCACATGCTTAAAG+TGG + tig0027465:27783-27802 None:intergenic 40.0%
! GGTTTGAGTTTCCATTTAGC+GGG - tig0027465:27880-27899 Msa1434100:intron 40.0%
! GTTTGAGTTTCCATTTAGCG+GGG - tig0027465:27881-27900 Msa1434100:intron 40.0%
! TTTGAGTTTCCATTTAGCGG+GGG - tig0027465:27882-27901 Msa1434100:intron 40.0%
CTGATTCCACCTAATGGAAA+AGG - tig0027465:27940-27959 Msa1434100:intron 40.0%
! CAACAAAGGCCTTTTCCATT+AGG + tig0027465:27952-27971 None:intergenic 40.0%
TAACAGTTCAACCCGACAAT+TGG - tig0027465:28289-28308 Msa1434100:intron 40.0%
! AGCATTGAAAGCCAATTGTC+GGG + tig0027465:28303-28322 None:intergenic 40.0%
TCATCTCATGTCCAGATATC+AGG - tig0027465:28366-28385 Msa1434100:intron 40.0%
CATGTCCAGATATCAGGAAT+TGG - tig0027465:28372-28391 Msa1434100:intron 40.0%
CGAATCCAATTCCTGATATC+TGG + tig0027465:28380-28399 None:intergenic 40.0%
CTGACATGGATGAAAGCAAA+CGG - tig0027465:28456-28475 Msa1434100:intron 40.0%
TGACATGGATGAAAGCAAAC+GGG - tig0027465:28457-28476 Msa1434100:intron 40.0%
AACAAATCTGAATGTGGAGG+AGG - tig0027465:28747-28766 Msa1434100:intron 40.0%
ACTGATGAGAGAATTCAACC+CGG + tig0027465:28988-29007 None:intergenic 40.0%
AAGATTAACCAACCAGACCA+GGG - tig0027465:29053-29072 Msa1434100:intron 40.0%
!! ACAACATGCAGATTTTTGGG+CGG + tig0027465:29096-29115 None:intergenic 40.0%
CTGCATGTTGTGGTTCTTAA+TGG - tig0027465:29105-29124 Msa1434100:CDS 40.0%
GTTCTTAATGGGAAGCTTGA+GGG - tig0027465:29117-29136 Msa1434100:intron 40.0%
! GCTTAAACCCACTCTTTTTC+AGG - tig0027465:29286-29305 Msa1434100:CDS 40.0%
!! AGCCTTATTGGCTTCAAGAA+AGG + tig0027465:29333-29352 None:intergenic 40.0%
AAGCCAATAAGGCTTAAAGC+AGG - tig0027465:29339-29358 Msa1434100:exon 40.0%
! GTTCCTGCTTTAAGCCTTAT+TGG + tig0027465:29345-29364 None:intergenic 40.0%
CACGGTGTGGTTAACATTTA+TGG - tig0027465:29387-29406 Msa1434100:intron 40.0%
AGAATGAATGTGTGGATGTC+TGG + tig0027465:29464-29483 None:intergenic 40.0%
!! GCCTATGTTTATTTCTGCTG+TGG - tig0027465:29544-29563 Msa1434100:five_prime_UTR 40.0%
TTCCTCTTCCTCATCTTCAC+AGG - tig0027465:26731-26750 Msa1434100:three_prime_UTR 45.0%
TACCTGTGAAGATGAGGAAG+AGG + tig0027465:26736-26755 None:intergenic 45.0%
TGGTACCGCTATCACCAATT+AGG + tig0027465:26974-26993 None:intergenic 45.0%
TTGCAAATATGGGACACAGC+CGG - tig0027465:27329-27348 Msa1434100:intron 45.0%
AGTTCGAAATCGCTCTTGAC+CGG + tig0027465:27351-27370 None:intergenic 45.0%
TCTCACGTCCTAATGTTCTC+TGG - tig0027465:27472-27491 Msa1434100:CDS 45.0%
TTGAGTTTCCATTTAGCGGG+GGG - tig0027465:27883-27902 Msa1434100:intron 45.0%
! CAAAGGCCTTTTCCATTAGG+TGG + tig0027465:27949-27968 None:intergenic 45.0%
CGGTAGTAAGCTGCAAAAGA+AGG + tig0027465:28088-28107 None:intergenic 45.0%
! TCTTTTGCAGCTTACTACCG+TGG - tig0027465:28088-28107 Msa1434100:intron 45.0%
GTAAAATACCCATGGCTCCA+CGG + tig0027465:28108-28127 None:intergenic 45.0%
CAGCCTAAAGGGAACATTGA+TGG + tig0027465:28572-28591 None:intergenic 45.0%
AGAAGTTGGCACAGCCTAAA+GGG + tig0027465:28583-28602 None:intergenic 45.0%
TAGAAGTTGGCACAGCCTAA+AGG + tig0027465:28584-28603 None:intergenic 45.0%
TTAGGCTGTGCCAACTTCTA+AGG - tig0027465:28584-28603 Msa1434100:intron 45.0%
TAGGCTGTGCCAACTTCTAA+GGG - tig0027465:28585-28604 Msa1434100:intron 45.0%
CATATTCATCTGCAAGCGCT+TGG + tig0027465:28611-28630 None:intergenic 45.0%
CAAGCGCTTGCAGATGAATA+TGG - tig0027465:28609-28628 Msa1434100:intron 45.0%
TGGGGCTGCAAAGAACAAAA+TGG + tig0027465:29026-29045 None:intergenic 45.0%
CAAGATTAACCAACCAGACC+AGG - tig0027465:29052-29071 Msa1434100:intron 45.0%
GCCCAAAAATCTGCATGTTG+TGG - tig0027465:29095-29114 Msa1434100:CDS 45.0%
GGTTCTTAATGGGAAGCTTG+AGG - tig0027465:29116-29135 Msa1434100:CDS 45.0%
ACAGTTGCTACTCATTGGGT+TGG - tig0027465:29212-29231 Msa1434100:intron 45.0%
!! TGCTACTCATTGGGTTGGAA+TGG - tig0027465:29217-29236 Msa1434100:intron 45.0%
ACCACACCATACTTGGCTTA+TGG - tig0027465:29363-29382 Msa1434100:intron 45.0%
ACCATAAGCCAAGTATGGTG+TGG + tig0027465:29367-29386 None:intergenic 45.0%
CCATACTTGGCTTATGGTCA+CGG - tig0027465:29369-29388 Msa1434100:intron 45.0%
! AGTTTGGAGTTTCAAAGGCG+TGG - tig0027465:29486-29505 Msa1434100:intron 45.0%
!! TATCTAATAATAATAATAAT+TGG + tig0027465:26800-26819 None:intergenic 5.0%
TAATGATCGGACACTCGCAG+AGG - tig0027465:26872-26891 Msa1434100:three_prime_UTR 50.0%
ATCGTAATCAGCACGAGCTC+TGG + tig0027465:26934-26953 None:intergenic 50.0%
CCAAGCCCCTGAAAAAGAGT+GGG + tig0027465:29296-29315 None:intergenic 50.0%
ACCAAGCCCCTGAAAAAGAG+TGG + tig0027465:29297-29316 None:intergenic 50.0%
AGCAGGAACCACACCATACT+TGG - tig0027465:29356-29375 Msa1434100:intron 50.0%
CCGTGACCATAAGCCAAGTA+TGG + tig0027465:29372-29391 None:intergenic 50.0%
GTGGATGTCTGGCATCAATG+TGG + tig0027465:29453-29472 None:intergenic 50.0%
TCGGACACTCGCAGAGGAAT+AGG - tig0027465:26878-26897 Msa1434100:three_prime_UTR 55.0%
! TAATCAGCACGAGCTCTGGC+AGG + tig0027465:26930-26949 None:intergenic 55.0%
ATGTCTTGCCCCCCGCTAAA+TGG + tig0027465:27894-27913 None:intergenic 55.0%
AGCTTACTACCGTGGAGCCA+TGG - tig0027465:28096-28115 Msa1434100:CDS 55.0%
GCTTACTACCGTGGAGCCAT+GGG - tig0027465:28097-28116 Msa1434100:CDS 55.0%
GGTGGGCAACAAAGCTGACA+TGG - tig0027465:28442-28461 Msa1434100:intron 55.0%
AGCGCTTGGCCCTTAGAAGT+TGG + tig0027465:28597-28616 None:intergenic 55.0%
!! TGCAGATTTTTGGGCGGCCT+GGG + tig0027465:29090-29109 None:intergenic 55.0%
!! ATGCAGATTTTTGGGCGGCC+TGG + tig0027465:29091-29110 None:intergenic 55.0%
!!! CCCACTCTTTTTCAGGGGCT+TGG - tig0027465:29293-29312 Msa1434100:CDS 55.0%
CTTGGCTTATGGTCACGGTG+TGG - tig0027465:29374-29393 Msa1434100:intron 55.0%
AACCAACCAGACCAGGGAGC+AGG - tig0027465:29059-29078 Msa1434100:intron 60.0%
ACCAACCAGACCAGGGAGCA+GGG - tig0027465:29060-29079 Msa1434100:intron 60.0%
! TCAGCACGAGCTCTGGCAGG+TGG + tig0027465:26927-26946 None:intergenic 65.0%
CCAACCAGACCAGGGAGCAG+GGG - tig0027465:29061-29080 Msa1434100:intron 65.0%
CCCCTGCTCCCTGGTCTGGT+TGG + tig0027465:29064-29083 None:intergenic 70.0%
GCAGCCCCTGCTCCCTGGTC+TGG + tig0027465:29068-29087 None:intergenic 75.0%
! CCTGGGCAGCCCCTGCTCCC+TGG + tig0027465:29073-29092 None:intergenic 80.0%
! CCAGGGAGCAGGGGCTGCCC+AGG - tig0027465:29070-29089 Msa1434100:CDS 80.0%
Chromosome Type Strat End Strand Name
tig0027465 gene 26565 29690 26565 ID=Msa1434100;Name=Msa1434100
tig0027465 mRNA 26565 29690 26565 ID=Msa1434100-mRNA-1;Parent=Msa1434100;Name=Msa1434100-mRNA-1;_AED=0.13;_eAED=0.13;_QI=211|1|1|1|0.87|0.77|9|566|216
tig0027465 exon 26565 27214 26565 ID=Msa1434100-mRNA-1:exon:8978;Parent=Msa1434100-mRNA-1
tig0027465 exon 27424 27516 27424 ID=Msa1434100-mRNA-1:exon:8977;Parent=Msa1434100-mRNA-1
tig0027465 exon 27605 27667 27605 ID=Msa1434100-mRNA-1:exon:8976;Parent=Msa1434100-mRNA-1
tig0027465 exon 27777 27874 27777 ID=Msa1434100-mRNA-1:exon:8975;Parent=Msa1434100-mRNA-1
tig0027465 exon 28092 28157 28092 ID=Msa1434100-mRNA-1:exon:8974;Parent=Msa1434100-mRNA-1
tig0027465 exon 28875 28975 28875 ID=Msa1434100-mRNA-1:exon:8973;Parent=Msa1434100-mRNA-1
tig0027465 exon 29063 29135 29063 ID=Msa1434100-mRNA-1:exon:8972;Parent=Msa1434100-mRNA-1
tig0027465 exon 29268 29363 29268 ID=Msa1434100-mRNA-1:exon:8971;Parent=Msa1434100-mRNA-1
tig0027465 exon 29503 29690 29503 ID=Msa1434100-mRNA-1:exon:8970;Parent=Msa1434100-mRNA-1
tig0027465 five_prime_UTR 29503 29690 29503 ID=Msa1434100-mRNA-1:five_prime_utr;Parent=Msa1434100-mRNA-1
tig0027465 five_prime_UTR 29341 29363 29341 ID=Msa1434100-mRNA-1:five_prime_utr;Parent=Msa1434100-mRNA-1
tig0027465 CDS 29268 29340 29268 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 29063 29135 29063 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 28875 28975 28875 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 28092 28157 28092 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 27777 27874 27777 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 27605 27667 27605 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 27424 27516 27424 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 CDS 27131 27214 27131 ID=Msa1434100-mRNA-1:cds;Parent=Msa1434100-mRNA-1
tig0027465 three_prime_UTR 26565 27130 26565 ID=Msa1434100-mRNA-1:three_prime_utr;Parent=Msa1434100-mRNA-1
Gene Sequence

>Msa1434100

ATGGCTGCTCCACCTGCCAGAGCTCGTGCTGATTACGATTATCTCATTAAACTTCTCCTAATTGGTGATAGCGGTGTTGGTAAAAGTTGTCTTTTATTGCGTTTCTCTGATGGGTCTTTTACAACTAGTTTTATCACAACCATTGGCATTGATTTCAAAATAAGGACAATTGAGCTTGATGGAAAGCGAATCAAATTGCAAATATGGGACACAGCCGGTCAAGAGCGATTTCGAACTATCACAACTGCTTACTACCGTGGAGCCATGGGTATTTTACTTGTATATGACGTTACGGACGAGTCTTCTTTTAACAATATCAGGAATTGGATTCGTAACATTGAGCAGCATGCTTCTGACAATGTTAACAAGATATTGGTGGGCAACAAAGCTGACATGGATGAAAGCAAACGGGCTGTGCCAACTTCTAAGGGCCAAGCGCTTGCAGATGAATATGGTATCAAGTTTTTTGAGACTAGTGCTAAAACAAATCTGAATGTGGAGGAGGTTTTCTTTTCAATAGCTCGGGACATCAAGCAAAGACTTGCAGATACTGACTCAAAATCTGAGCCCCAGATAATCAAGATTAACCAACCAGACCAGGGAGCAGGGGCTGCCCAGGCCGCCCAAAAATCTGCATGTTGTGGTTCTTAA
Protein sequence

>Msa1434100

MAAPPARARADYDYLIKLLLIGDSGVGKSCLLLRFSDGSFTTSFITTIGIDFKIRTIELDGKRIKLQIWDTAGQERFRTITTAYYRGAMGILLVYDVTDESSFNNIRNWIRNIEQHASDNVNKILVGNKADMDESKRAVPTSKGQALADEYGIKFFETSAKTNLNVEEVFFSIARDIKQRLADTDSKSEPQIIKINQPDQGAGAAQAAQKSACCGS*