AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0245580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0245580 MtrunA17_Chr2g0299681 99.516 413 0 1 1 411 1 413 0.0 842
Msa0245580 MtrunA17_Chr4g0070981 92.978 413 27 1 1 411 1 413 0.0 795
Msa0245580 MtrunA17_Chr2g0329931 92.494 413 29 1 1 411 1 413 0.0 792
Msa0245580 MtrunA17_Chr8g0342861 92.252 413 30 1 1 411 1 413 0.0 788
Msa0245580 MtrunA17_Chr2g0297261 65.952 373 125 1 41 411 34 406 0.0 518
Msa0245580 MtrunA17_Chr1g0169331 60.406 394 117 6 56 411 6 398 5.56e-159 453
Msa0245580 MtrunA17_Chr8g0342851 63.006 346 94 5 71 411 3 319 1.23e-140 404
Msa0245580 MtrunA17_Chr7g0247191 40.872 367 213 3 40 404 149 513 8.90e-89 278
Msa0245580 MtrunA17_Chr7g0247181 40.054 367 216 3 40 404 139 503 3.92e-87 274
Msa0245580 MtrunA17_Chr7g0247221 40.054 367 216 3 40 404 142 506 4.62e-87 273
Msa0245580 MtrunA17_Chr4g0003441 39.674 368 214 6 47 407 114 480 1.09e-76 246
Msa0245580 MtrunA17_Chr1g0149251 38.872 337 198 6 78 407 1 336 1.74e-70 225
Msa0245580 MtrunA17_Chr1g0179761 36.634 404 216 13 41 409 80 478 7.79e-69 225
Msa0245580 MtrunA17_Chr3g0107851 35.484 372 222 4 40 394 116 486 1.25e-67 224
Msa0245580 MtrunA17_Chr3g0095031 36.364 374 227 6 45 409 94 465 7.23e-66 219
Msa0245580 MtrunA17_Chr7g0271121 39.118 340 194 7 66 394 131 468 1.91e-65 222
Msa0245580 MtrunA17_Chr5g0442191 34.239 368 233 4 45 404 85 451 5.19e-63 213
Msa0245580 MtrunA17_Chr1g0193491 34.286 385 229 6 27 394 100 477 6.56e-63 213
Msa0245580 MtrunA17_Chr2g0330401 34.615 364 222 5 41 392 96 455 6.83e-62 207
Msa0245580 MtrunA17_Chr4g0076491 32.927 410 228 8 17 385 254 657 5.93e-60 206
Msa0245580 MtrunA17_Chr6g0483491 33.154 371 242 4 41 406 10 379 1.14e-59 200
Msa0245580 MtrunA17_Chr7g0236841 33.154 371 242 4 41 406 10 379 3.15e-59 199
Msa0245580 MtrunA17_Chr3g0121351 33.846 390 233 9 40 405 144 532 2.43e-58 201
Msa0245580 MtrunA17_Chr6g0449741 34.688 369 205 7 47 385 255 617 6.69e-58 200
Msa0245580 MtrunA17_Chr7g0270131 34.615 364 223 8 41 392 232 592 7.45e-58 202
Msa0245580 MtrunA17_Chr4g0040761 32.258 372 242 5 42 405 20 389 2.40e-55 195
Msa0245580 MtrunA17_Chr4g0063551 33.673 392 229 7 42 405 141 529 2.66e-55 193
Msa0245580 MtrunA17_Chr2g0310581 34.167 360 230 4 41 393 53 412 6.15e-55 189
Msa0245580 MtrunA17_Chr1g0157691 30.848 389 248 8 41 411 147 532 7.72e-55 191
Msa0245580 MtrunA17_Chr3g0098121 34.153 366 227 6 40 394 151 513 3.10e-54 192
Msa0245580 MtrunA17_Chr2g0277821 34.015 391 226 9 41 410 504 883 5.00e-54 193
Msa0245580 MtrunA17_Chr1g0200181 30.848 389 248 8 41 411 145 530 6.04e-54 188
Msa0245580 MtrunA17_Chr6g0449911 32.105 380 245 6 41 410 165 541 1.45e-53 189
Msa0245580 MtrunA17_Chr2g0293631 33.702 362 231 6 41 394 496 856 3.50e-53 191
Msa0245580 MtrunA17_Chr1g0176041 30.465 430 263 9 12 405 111 540 4.55e-53 186
Msa0245580 MtrunA17_Chr5g0409981 33.943 383 231 9 36 401 96 473 4.21e-52 182
Msa0245580 MtrunA17_Chr2g0300291 32.898 383 239 8 27 394 124 503 2.55e-49 178
Msa0245580 MtrunA17_Chr6g0461751 31.868 364 234 7 41 392 140 501 5.29e-47 169
Msa0245580 MtrunA17_Chr2g0307471 30.435 368 231 10 40 389 90 450 4.66e-44 161
Msa0245580 MtrunA17_Chr4g0062301 30.618 356 227 10 50 389 106 457 2.69e-43 159
Msa0245580 MtrunA17_Chr7g0263771 28.535 389 253 6 34 410 129 504 4.29e-42 156
Msa0245580 MtrunA17_Chr6g0475251 30.769 351 214 7 45 378 115 453 4.93e-42 157
Msa0245580 MtrunA17_Chr2g0309551 25.962 416 245 7 41 394 109 523 9.47e-41 152
Msa0245580 MtrunA17_Chr8g0347171 27.960 397 236 10 42 404 161 541 1.10e-38 147
Msa0245580 MtrunA17_Chr8g0347161 28.792 389 243 10 42 404 103 483 1.69e-38 145
Msa0245580 MtrunA17_Chr4g0058591 31.715 309 203 4 41 341 202 510 1.90e-38 145
Msa0245580 MtrunA17_Chr3g0114081 28.378 370 233 8 47 386 22 389 3.54e-38 145
Msa0245580 MtrunA17_Chr8g0347151 27.960 397 231 10 42 404 202 577 6.17e-38 145
Msa0245580 MtrunA17_Chr1g0204391 29.268 369 216 7 47 378 50 410 6.24e-38 144
Msa0245580 MtrunA17_Chr8g0386451 27.865 384 253 7 42 404 289 669 8.77e-38 145
Msa0245580 MtrunA17_Chr7g0236831 25.624 441 240 11 39 397 161 595 5.74e-36 140
Msa0245580 MtrunA17_Chr4g0025141 36.715 207 116 5 212 403 14 220 2.33e-35 130
Msa0245580 MtrunA17_Chr1g0192021 26.570 414 237 9 41 395 26 431 5.10e-35 136
Msa0245580 MtrunA17_Chr6g0466611 28.684 380 241 11 41 394 142 517 1.17e-33 133
Msa0245580 MtrunA17_Chr3g0137901 40.244 164 96 2 245 407 10 172 4.20e-33 122
Msa0245580 MtrunA17_Chr4g0034411 28.488 344 221 10 68 393 102 438 6.43e-33 131
Msa0245580 MtrunA17_Chr5g0443991 27.642 369 238 10 68 411 102 466 3.40e-32 129
Msa0245580 MtrunA17_Chr4g0029191 25.676 444 245 11 41 401 44 485 8.17e-31 124
Msa0245580 MtrunA17_Chr5g0429221 26.975 367 246 8 41 394 3 360 6.79e-27 111
Msa0245580 MtrunA17_Chr3g0137891 40.994 161 90 3 78 233 1 161 1.16e-26 106
Msa0245580 MtrunA17_Chr4g0074521 26.854 391 236 14 49 399 90 470 4.59e-25 106
Msa0245580 MtrunA17_Chr3g0145781 26.866 402 236 13 24 381 120 507 6.12e-25 106
Msa0245580 MtrunA17_Chr4g0025101 30.672 238 99 7 196 409 7 202 3.67e-24 99.8
Msa0245580 MtrunA17_Chr4g0039471 36.139 202 121 4 25 221 210 408 4.62e-24 103
Msa0245580 MtrunA17_Chr4g0062321 29.487 234 157 6 117 346 4 233 5.48e-23 97.1
Msa0245580 MtrunA17_Chr4g0004301 25.926 378 209 13 79 387 71 446 5.66e-23 100
Msa0245580 MtrunA17_Chr4g0025131 33.708 178 110 5 24 195 40 215 2.80e-22 95.9
Msa0245580 MtrunA17_Chr5g0434301 25.661 378 210 13 79 387 71 446 3.18e-22 98.6
Msa0245580 MtrunA17_Chr2g0301281 30.939 181 114 4 41 210 41 221 2.36e-18 87.4
Msa0245580 MtrunA17_Chr2g0301281 35.052 97 62 1 308 404 389 484 1.95e-14 75.1
Msa0245580 MtrunA17_Chr8g0349951 37.500 136 82 2 41 173 10 145 1.07e-17 79.7
Msa0245580 MtrunA17_Chr8g0354761 24.225 355 247 10 64 402 31 379 1.14e-17 84.7
Msa0245580 MtrunA17_Chr1g0155201 28.980 245 143 10 158 393 13 235 1.71e-17 83.2
Msa0245580 MtrunA17_Chr0c30g0494091 26.718 262 160 9 49 285 90 344 1.83e-12 68.6
Msa0245580 MtrunA17_Chr1g0155691 29.703 101 69 1 311 411 37 135 1.71e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0245580 AT3G13920.1 91.063 414 32 3 1 411 1 412 0.0 774
Msa0245580 AT1G54270.1 90.821 414 33 3 1 411 1 412 0.0 772
Msa0245580 AT1G72730.1 87.923 414 47 2 1 411 1 414 0.0 758
Msa0245580 AT3G13920.4 89.855 414 32 4 1 411 1 407 0.0 757
Msa0245580 AT1G54270.2 89.614 414 33 4 1 411 1 407 0.0 755
Msa0245580 AT3G13920.3 92.969 384 25 1 30 411 19 402 0.0 740
Msa0245580 AT3G13920.5 91.349 393 29 3 1 390 5 395 0.0 734
Msa0245580 AT3G13920.2 91.349 393 29 3 1 390 1 391 0.0 734
Msa0245580 AT3G19760.1 65.416 373 127 1 41 411 36 408 0.0 512
Msa0245580 AT1G51380.1 59.621 369 147 1 41 407 23 391 1.55e-163 465
Msa0245580 AT1G51380.2 59.524 252 100 1 41 290 23 274 7.50e-108 319
Msa0245580 AT3G61240.2 40.000 370 218 3 40 407 124 491 7.09e-90 281
Msa0245580 AT3G61240.1 40.000 370 218 3 40 407 124 491 7.09e-90 281
Msa0245580 AT4G00660.1 39.189 370 221 3 40 407 131 498 1.83e-88 277
Msa0245580 AT4G00660.2 39.189 370 221 3 40 407 131 498 1.83e-88 277
Msa0245580 AT2G45810.1 40.217 368 216 3 40 405 154 519 3.84e-88 277
Msa0245580 AT1G51380.4 56.872 211 89 1 41 249 23 233 1.71e-81 253
Msa0245580 AT1G51380.3 56.872 211 89 1 41 249 23 233 2.89e-81 252
Msa0245580 AT5G11170.1 39.674 368 214 6 47 407 53 419 4.25e-78 248
Msa0245580 AT5G11200.1 39.674 368 214 6 47 407 53 419 4.25e-78 248
Msa0245580 AT5G11170.2 39.169 337 197 6 78 407 1 336 3.89e-70 225
Msa0245580 AT5G11200.3 35.697 409 214 7 47 407 53 460 1.63e-69 227
Msa0245580 AT3G53110.1 35.338 399 230 11 33 405 81 477 2.50e-69 227
Msa0245580 AT5G26742.1 34.532 417 248 8 9 411 80 485 7.00e-67 226
Msa0245580 AT5G26742.2 34.532 417 248 8 9 411 80 485 7.10e-67 226
Msa0245580 AT3G22310.2 35.389 373 232 4 45 409 121 492 1.36e-66 223
Msa0245580 AT3G22310.1 35.389 373 232 4 45 409 121 492 1.82e-66 223
Msa0245580 AT5G26742.3 37.465 355 206 7 71 411 40 392 1.90e-66 223
Msa0245580 AT3G22330.1 35.389 373 232 4 45 409 109 480 1.96e-66 223
Msa0245580 AT2G33730.1 33.886 422 242 8 10 395 274 694 2.63e-63 216
Msa0245580 AT5G63120.2 34.921 378 238 4 42 411 167 544 3.63e-63 213
Msa0245580 AT1G55150.1 34.146 369 232 5 41 401 100 465 8.50e-62 207
Msa0245580 AT1G55150.2 34.146 369 232 5 41 401 125 490 1.42e-61 207
Msa0245580 AT1G77030.1 32.258 372 242 5 42 405 30 399 5.81e-58 203
Msa0245580 AT5G14610.6 34.127 378 240 6 41 410 162 538 9.81e-58 200
Msa0245580 AT5G14610.5 34.127 378 240 6 41 410 170 546 1.03e-57 200
Msa0245580 AT5G14610.2 34.127 378 240 6 41 410 162 538 1.06e-57 200
Msa0245580 AT3G01540.4 35.789 380 231 10 41 410 159 535 2.63e-57 198
Msa0245580 AT3G01540.2 35.789 380 231 10 41 410 159 535 2.63e-57 198
Msa0245580 AT3G01540.3 35.789 380 231 10 41 410 159 535 2.63e-57 198
Msa0245580 AT3G01540.1 35.789 380 231 10 41 410 159 535 3.05e-57 198
Msa0245580 AT5G60990.1 32.225 391 249 4 36 411 5 394 9.87e-57 193
Msa0245580 AT1G20920.2 34.218 377 234 7 46 410 197 571 2.06e-56 199
Msa0245580 AT2G47330.1 34.197 386 231 10 41 410 229 607 4.99e-56 197
Msa0245580 AT1G16280.1 33.795 361 232 3 41 394 59 419 7.29e-56 192
Msa0245580 AT3G06480.1 33.770 382 240 7 39 410 434 812 1.30e-55 198
Msa0245580 AT3G58570.1 32.832 399 239 8 33 404 136 532 4.11e-55 193
Msa0245580 AT2G42520.2 32.304 421 249 11 16 404 129 545 5.39e-55 192
Msa0245580 AT2G42520.1 32.304 421 249 11 16 404 129 545 5.39e-55 192
Msa0245580 AT2G42520.3 32.304 421 249 11 16 404 129 545 5.39e-55 192
Msa0245580 AT1G20920.4 34.565 379 230 8 46 410 535 909 7.02e-55 196
Msa0245580 AT1G20920.6 34.565 379 230 8 46 410 535 909 7.02e-55 196
Msa0245580 AT1G20920.3 34.565 379 230 8 46 410 535 909 7.02e-55 196
Msa0245580 AT1G20920.7 34.565 379 230 8 46 410 535 909 7.02e-55 196
Msa0245580 AT1G20920.5 34.565 379 230 8 46 410 535 909 7.02e-55 196
Msa0245580 AT1G20920.1 34.565 379 230 8 46 410 535 909 7.02e-55 196
Msa0245580 AT3G58510.1 31.959 388 238 8 40 404 151 535 7.48e-55 191
Msa0245580 AT3G58510.3 31.959 388 238 8 40 404 151 535 7.48e-55 191
Msa0245580 AT3G58510.2 31.959 388 238 8 40 404 151 535 7.48e-55 191
Msa0245580 AT4G16630.1 34.133 375 236 5 31 394 155 529 1.14e-54 194
Msa0245580 AT5G14610.7 32.967 364 235 6 55 410 45 407 1.13e-53 186
Msa0245580 AT5G14610.3 32.967 364 235 6 55 410 45 407 1.13e-53 186
Msa0245580 AT4G33370.1 31.701 388 244 8 41 410 98 482 8.40e-53 185
Msa0245580 AT5G51280.1 30.670 388 248 8 41 410 147 531 9.14e-53 186
Msa0245580 AT3G02065.3 32.603 365 231 6 41 392 111 473 2.14e-52 183
Msa0245580 AT3G02065.2 32.603 365 231 6 41 392 111 473 2.14e-52 183
Msa0245580 AT5G14610.1 33.333 366 235 6 53 410 242 606 2.52e-52 186
Msa0245580 AT1G28180.1 34.078 358 211 8 63 408 5 349 2.40e-50 174
Msa0245580 AT1G31970.1 32.979 376 232 9 41 401 117 487 5.55e-50 177
Msa0245580 AT3G18600.1 31.842 380 247 8 41 410 90 467 2.79e-49 176
Msa0245580 AT3G09720.2 30.435 368 252 3 46 410 147 513 8.30e-49 174
Msa0245580 AT3G09720.1 30.435 368 252 3 46 410 147 513 8.30e-49 174
Msa0245580 AT1G12770.2 28.218 404 220 7 41 379 111 509 3.23e-48 172
Msa0245580 AT1G12770.1 28.218 404 220 7 41 379 111 509 3.23e-48 172
Msa0245580 AT3G09620.1 32.888 374 208 9 46 407 402 744 1.44e-47 174
Msa0245580 AT3G09620.2 32.888 374 208 9 46 407 403 745 1.47e-47 174
Msa0245580 AT3G02065.1 32.931 331 207 6 75 392 8 336 2.88e-47 166
Msa0245580 AT5G65900.1 31.367 373 243 7 41 402 155 525 3.76e-45 165
Msa0245580 AT5G62190.1 31.351 370 228 9 55 405 111 473 5.17e-45 166
Msa0245580 AT5G63120.1 34.416 308 194 4 42 341 167 474 2.32e-43 158
Msa0245580 AT5G63120.3 34.416 308 194 4 42 341 167 474 2.87e-43 158
Msa0245580 AT5G63120.4 34.416 308 194 4 42 341 167 474 2.87e-43 158
Msa0245580 AT5G63120.5 34.416 308 194 4 42 341 167 474 2.87e-43 158
Msa0245580 AT5G05450.1 29.973 367 228 9 47 386 23 387 4.79e-42 156
Msa0245580 AT5G63630.1 30.295 373 239 7 42 393 56 428 8.10e-41 152
Msa0245580 AT1G71370.1 30.028 353 227 9 47 381 23 373 9.80e-41 152
Msa0245580 AT5G14610.4 31.915 329 215 6 41 361 170 497 1.24e-40 151
Msa0245580 AT5G63630.2 30.295 373 239 7 42 393 250 622 1.19e-39 150
Msa0245580 AT5G08620.1 30.208 384 225 9 42 393 82 454 1.60e-39 149
Msa0245580 AT5G08610.1 30.026 383 227 9 42 393 384 756 9.10e-39 149
Msa0245580 AT3G06480.2 31.988 322 206 7 39 350 434 752 1.42e-38 148
Msa0245580 AT2G07750.1 27.543 403 249 11 35 405 368 759 8.11e-38 146
Msa0245580 AT1G63250.1 27.295 403 250 10 35 405 321 712 3.94e-36 141
Msa0245580 AT5G11200.2 38.496 226 123 5 47 265 53 269 1.18e-34 134
Msa0245580 AT5G11200.2 38.506 174 104 3 236 407 306 478 1.89e-30 122
Msa0245580 AT5G54910.1 28.030 396 258 12 17 394 49 435 2.67e-33 132
Msa0245580 AT4G34910.1 25.791 411 246 8 41 395 47 454 2.79e-33 132
Msa0245580 AT2G40700.1 25.450 444 248 12 41 401 31 474 5.94e-32 128
Msa0245580 AT5G19210.2 27.551 392 232 13 49 400 85 464 2.65e-31 125
Msa0245580 AT4G09730.1 25.515 388 251 9 37 394 109 488 3.26e-30 123
Msa0245580 AT3G16840.1 25.968 439 236 13 39 398 189 617 1.09e-29 122
Msa0245580 AT3G16840.2 25.968 439 236 13 39 398 190 618 1.23e-29 122
Msa0245580 AT1G71280.2 28.710 310 183 10 47 343 24 308 5.78e-28 113
Msa0245580 AT1G71280.1 29.032 310 182 10 47 343 24 308 3.32e-27 113
Msa0245580 AT3G06980.1 28.232 379 230 13 41 381 375 749 6.05e-27 114
Msa0245580 AT4G15850.1 25.648 386 209 14 79 392 74 453 8.23e-25 106
Msa0245580 AT5G19210.1 26.006 323 177 13 123 400 2 307 7.54e-17 81.3
Msa0245580 AT5G19210.3 26.006 323 177 13 123 400 2 307 7.54e-17 81.3
Msa0245580 AT1G28180.2 37.398 123 58 4 63 175 5 118 5.51e-12 63.2

Find 128 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGATAATGTCCCTAGTATTT+TGG 0.210988 2_2:+25361369 None:intergenic
GTTGGATGTGTTTGTTTATT+GGG 0.211604 2_2:-25360733 Msa0245580:three_prime_UTR
CGTTGGATGTGTTTGTTTAT+TGG 0.222272 2_2:-25360734 Msa0245580:three_prime_UTR
ACATCAAGACCCTTGATAAA+TGG 0.268068 2_2:+25362823 None:intergenic
AATTACTACACCTATTAAAA+AGG 0.284543 2_2:+25360951 None:intergenic
TTCAAATCCGGCTACTTTGC+AGG 0.294985 2_2:+25360414 None:intergenic
ATACAACAAATGGGGCAAAA+AGG 0.311464 2_2:+25360997 None:intergenic
TTTGATAACTACCGATCTTC+TGG 0.320168 2_2:-25361315 Msa0245580:CDS
TCAACACATTGTAGAACTTC+TGG 0.325013 2_2:+25361129 None:intergenic
TAAAACAGAAATACAACAAA+TGG 0.332402 2_2:+25360987 None:intergenic
TGATGTTCAGCAAGTGTCTT+TGG 0.336264 2_2:-25361282 Msa0245580:CDS
CTCAGAGGCATTTACGCATA+TGG 0.350883 2_2:-25363427 Msa0245580:intron
TTTCTTCCATCATTACTTAG+AGG 0.353720 2_2:-25361060 Msa0245580:three_prime_UTR
CTGTGATCTTTATGAGACTT+TGG 0.355425 2_2:-25361501 Msa0245580:CDS
GTCAAAGGTTACATAAGTTA+TGG 0.381297 2_2:-25360690 Msa0245580:three_prime_UTR
AATAATATTGTGCAGCAATC+AGG 0.388150 2_2:+25360639 None:intergenic
CAACAGGCTCAGTCTGGAAC+AGG 0.390696 2_2:-25362796 Msa0245580:CDS
ATCATGCGTGAATTCCGTTC+TGG 0.390799 2_2:-25361350 Msa0245580:CDS
TAGTAGACTGTTTCTATTGA+AGG 0.392354 2_2:-25360528 Msa0245580:three_prime_UTR
GCCATCAGCTATTCAGCAAC+GGG 0.393795 2_2:-25362854 Msa0245580:CDS
AATGTGTTGATCATTTCAAT+TGG 0.396260 2_2:+25360553 None:intergenic
GAATGCATTATTGTCTGAAT+TGG 0.400654 2_2:-25360877 Msa0245580:three_prime_UTR
ACTCCGACTTGAATCTTAGA+TGG 0.408502 2_2:+25361680 None:intergenic
AAGACTGCAACCTTCTGCTC+TGG 0.409029 2_2:-25362772 Msa0245580:CDS
AAAACAGAAATACAACAAAT+GGG 0.412064 2_2:+25360988 None:intergenic
TTACATCAAAATGTTCGTAT+TGG 0.414807 2_2:-25362497 Msa0245580:CDS
ATCATTACTTAGAGGGAAGT+TGG 0.426487 2_2:-25361052 Msa0245580:three_prime_UTR
GGGAATAGTTCCATTTATCA+AGG 0.444746 2_2:-25362833 Msa0245580:CDS
CATGTTGTTGTTGGTACCCC+TGG 0.445958 2_2:-25362562 Msa0245580:CDS
AGCATGTCAAATACACGTCC+AGG 0.452941 2_2:+25362544 None:intergenic
CTTGTGATCTCAAGTGCCTC+AGG 0.456300 2_2:+25361638 None:intergenic
ATTGGTCGTAGTGGAAGGTT+TGG 0.459303 2_2:-25361212 Msa0245580:CDS
GGGTCTTGATGTTATACAAC+AGG 0.460845 2_2:-25362812 Msa0245580:CDS
TCCTTTACTTCCTGCAGTCA+TGG 0.461248 2_2:-25363743 Msa0245580:exon
GATAATTAGTGTCGAATAGC+AGG 0.462378 2_2:-25360449 Msa0245580:three_prime_UTR
GAGACATGGACCAAAATACT+AGG 0.463229 2_2:-25361379 Msa0245580:CDS
GTTATACAACAGGCTCAGTC+TGG 0.464551 2_2:-25362802 Msa0245580:CDS
TTACTTCCTGCAGTCATGGC+AGG 0.465723 2_2:-25363739 Msa0245580:exon
GATAGTTCTCAGGCTGTGTT+GGG 0.470642 2_2:+25361243 None:intergenic
CGTGCACTTGGAGATTATCT+AGG 0.472296 2_2:-25362655 Msa0245580:CDS
AGCGGTGTTCATGTTGTTGT+TGG 0.477024 2_2:-25362571 Msa0245580:CDS
AACTTCCCTCTAAGTAATGA+TGG 0.477500 2_2:+25361054 None:intergenic
CGAATGCCAAGCCTTGGTCT+TGG 0.479480 2_2:-25362719 Msa0245580:CDS
CAAGACCAAGGCTTGGCATT+CGG 0.481084 2_2:+25362720 None:intergenic
GTTCTACAATGTGTTGATTG+AGG 0.485517 2_2:-25361123 Msa0245580:CDS
GCGTGATGAGCTCACCTTGG+AGG 0.488852 2_2:-25361579 Msa0245580:CDS
TCACAGAAGTTCAGCAACAT+TGG 0.492163 2_2:+25361091 None:intergenic
GAGAAGGTCATGCGTGCACT+TGG 0.495619 2_2:-25362667 Msa0245580:CDS
CAGCCAGTCAACCTTTCGTC+TGG 0.496367 2_2:+25361448 None:intergenic
ACACCAGACGAAAGGTTGAC+TGG 0.497320 2_2:-25361451 Msa0245580:CDS
TTGGATGTGTTTGTTTATTG+GGG 0.499468 2_2:-25360732 Msa0245580:three_prime_UTR
ATTGCAAGTACTTATCACTT+AGG 0.507302 2_2:+25360903 None:intergenic
GCATCAAACTGTGAACCCTC+TGG 0.509050 2_2:+25363709 None:intergenic
AGACTGCAACCTTCTGCTCT+GGG 0.510787 2_2:-25362771 Msa0245580:CDS
ATGTGTTGATCATTTCAATT+GGG 0.510828 2_2:+25360554 None:intergenic
CCCCGTTGCTGAATAGCTGA+TGG 0.513832 2_2:+25362853 None:intergenic
AATAAACAAACACATCCAAC+GGG 0.514935 2_2:+25360736 None:intergenic
GAATCTTAGATGGCAACAGC+TGG 0.517992 2_2:+25361690 None:intergenic
CTCTGCTACAATGCCTCCTG+AGG 0.521626 2_2:-25361654 Msa0245580:CDS
GAGAACTATCTCCATCGTAT+TGG 0.523557 2_2:-25361230 Msa0245580:CDS
AGATAGTTCTCAGGCTGTGT+TGG 0.525373 2_2:+25361242 None:intergenic
TTCTTCCATCATTACTTAGA+GGG 0.526743 2_2:-25361059 Msa0245580:three_prime_UTR
ATCGTATTGGTCGTAGTGGA+AGG 0.529668 2_2:-25361217 Msa0245580:CDS
GCCATGACTGCAGGAAGTAA+AGG 0.529865 2_2:+25363742 None:intergenic
AGATGACTCGATTCGGTCAA+AGG 0.538285 2_2:-25360705 Msa0245580:three_prime_UTR
GCGCTGATCTTCACGAACAC+TGG 0.540854 2_2:+25362603 None:intergenic
CTCCATCGTATTGGTCGTAG+TGG 0.541717 2_2:-25361221 Msa0245580:CDS
GTCGTAGTGGAAGGTTTGGA+AGG 0.547854 2_2:-25361208 Msa0245580:CDS
GCAACGCCTGCCATGACTGC+AGG 0.548397 2_2:+25363733 None:intergenic
CGTGATGAGCTCACCTTGGA+GGG 0.552554 2_2:-25361578 Msa0245580:CDS
CATGGCAGGCGTTGCACCAG+AGG 0.552942 2_2:-25363725 Msa0245580:CDS
GCTCTTTAATTAAATGAACA+TGG 0.553365 2_2:-25360614 Msa0245580:three_prime_UTR
CTGCAAAATCCCAGAGCAGA+AGG 0.553669 2_2:+25362762 None:intergenic
CAATAAACAAACACATCCAA+CGG 0.554551 2_2:+25360735 None:intergenic
CAGACTCACAACTGATGCAC+AGG 0.557617 2_2:-25363515 Msa0245580:CDS
ATACGATGGAGATAGTTCTC+AGG 0.557654 2_2:+25361233 None:intergenic
CTCAGCAACACACGGAGACA+TGG 0.558453 2_2:-25361393 Msa0245580:CDS
AGTGGAAGGTTTGGAAGGAA+AGG 0.560979 2_2:-25361203 Msa0245580:CDS
CTGAGGCACTTGAGATCACA+AGG 0.563772 2_2:-25361637 Msa0245580:CDS
AAGTTGGTTTCTGAGTTAAG+TGG 0.564360 2_2:-25361036 Msa0245580:three_prime_UTR
TTATGTCAACGTTGATAAGG+AGG 0.566533 2_2:-25361543 Msa0245580:CDS
GGTGTATCGCTGGTACCCGT+TGG 0.568819 2_2:-25360751 Msa0245580:three_prime_UTR
TGTGACCGAATGCCAAGCCT+TGG 0.571337 2_2:-25362725 Msa0245580:CDS
TCAACGTTGATAAGGAGGAA+TGG 0.577711 2_2:-25361538 Msa0245580:CDS
CAAAATGTTCGTATTGGACG+AGG 0.578200 2_2:-25362491 Msa0245580:CDS
CGCTTCACAAGAATCCTCAC+AGG 0.579557 2_2:+25361599 None:intergenic
GTTGATCATTTCAATTGGGA+TGG 0.580324 2_2:+25360558 None:intergenic
TTGCTGAGACAGTGTGGTCA+CGG 0.580925 2_2:+25361408 None:intergenic
ATGGCAGGCGTTGCACCAGA+GGG 0.587343 2_2:-25363724 Msa0245580:CDS
GGAATAGTTCCATTTATCAA+GGG 0.589236 2_2:-25362832 Msa0245580:CDS
TGGATGTGTTTGTTTATTGG+GGG 0.589577 2_2:-25360731 Msa0245580:three_prime_UTR
AGAAGTTCAGCAACATTGGA+AGG 0.591345 2_2:+25361095 None:intergenic
AGCCATCAGCTATTCAGCAA+CGG 0.592072 2_2:-25362855 Msa0245580:CDS
TGTAAACAAAGGTGTATCGC+TGG 0.594178 2_2:-25360761 Msa0245580:three_prime_UTR
GGATGTGTTTGTTTATTGGG+GGG 0.594918 2_2:-25360730 Msa0245580:three_prime_UTR
CTTCTTCACATCTTATGATG+AGG 0.599921 2_2:-25363491 Msa0245580:CDS
GCATGTCAAATACACGTCCA+GGG 0.601935 2_2:+25362545 None:intergenic
GCTGATGAGATGCTTTCTCG+TGG 0.610128 2_2:-25362469 Msa0245580:CDS
ATCGGTAGTTATCAAAACAC+GGG 0.610300 2_2:+25361322 None:intergenic
CACACTGTCTCAGCAACACA+CGG 0.611599 2_2:-25361401 Msa0245580:CDS
TAGGTGCCAAGACCAAGGCT+TGG 0.617896 2_2:+25362713 None:intergenic
GATCGGTAGTTATCAAAACA+CGG 0.623883 2_2:+25361321 None:intergenic
GATCAGCGCATTCTATCAAG+CGG 0.624396 2_2:-25362589 Msa0245580:CDS
AGACATGGACCAAAATACTA+GGG 0.628384 2_2:-25361378 Msa0245580:CDS
AATACCACGAGCCAGAAGAT+CGG 0.631247 2_2:+25361304 None:intergenic
AAGGTGCATGCTTGTGTCGG+AGG 0.634488 2_2:-25362628 Msa0245580:CDS
GCTTGCTCAGCAGATTGAGA+AGG 0.634804 2_2:-25362683 Msa0245580:CDS
GTGAAGGTGCATGCTTGTGT+CGG 0.636750 2_2:-25362631 Msa0245580:intron
TTCCACTACGACCAATACGA+TGG 0.638034 2_2:+25361219 None:intergenic
GTGATCTCAAGTGCCTCAGG+AGG 0.642550 2_2:+25361641 None:intergenic
TAGACTGTTTCTATTGAAGG+CGG 0.645292 2_2:-25360525 Msa0245580:three_prime_UTR
ACTACCGATCTTCTGGCTCG+TGG 0.651230 2_2:-25361308 Msa0245580:CDS
AACAGGCTCAGTCTGGAACA+GGG 0.652293 2_2:-25362795 Msa0245580:CDS
AAACAGAAATACAACAAATG+GGG 0.652592 2_2:+25360989 None:intergenic
CATTACGAACATGCTACTTG+AGG 0.655050 2_2:+25360591 None:intergenic
ACGAGTAGGTGCCAAGACCA+AGG 0.655523 2_2:+25362708 None:intergenic
TGCTGAGCAAGCTCACGAGT+AGG 0.660506 2_2:+25362694 None:intergenic
AAACTGCTTGATACCCTCCA+AGG 0.661421 2_2:+25361565 None:intergenic
TTGCCATCTAAGATTCAAGT+CGG 0.662032 2_2:-25361683 Msa0245580:CDS
AGTGATAAGTACTTGCAATG+TGG 0.663450 2_2:-25360899 Msa0245580:three_prime_UTR
TATCACTTAGGATGTTCAGA+CGG 0.669632 2_2:+25360915 None:intergenic
GAAGCGTGATGAGCTCACCT+TGG 0.671691 2_2:-25361582 Msa0245580:CDS
CATGTCAAATACACGTCCAG+GGG 0.696747 2_2:+25362546 None:intergenic
TTGCAAGAGAATCTTCTCAG+AGG 0.702805 2_2:-25363442 Msa0245580:CDS
AAACACGGGAAGATCCAGAA+CGG 0.712720 2_2:+25361336 None:intergenic
CGTGTGTTGCTGAGACAGTG+TGG 0.721187 2_2:+25361402 None:intergenic
AGTTCATGAACAAACCTGTG+AGG 0.726622 2_2:-25361613 Msa0245580:CDS
CCATCAGCTATTCAGCAACG+GGG 0.741503 2_2:-25362853 Msa0245580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTCTTTATAATTTTTGAAAT+TGG - chr2_2:25360987-25361006 Msa0245580:three_prime_UTR 10.0%
!! TATTGTTAAAAAACAAATTA+AGG - chr2_2:25361893-25361912 Msa0245580:intron 10.0%
!! TGGTTAAAAAAATTAAAGAT+TGG + chr2_2:25360813-25360832 None:intergenic 15.0%
!!! TTTAATTTTTTTAACCAGAT+GGG - chr2_2:25360816-25360835 Msa0245580:three_prime_UTR 15.0%
!! AAGAATTAAAATGTTATTGT+TGG - chr2_2:25360863-25360882 Msa0245580:three_prime_UTR 15.0%
!! AGAATTAAAATGTTATTGTT+GGG - chr2_2:25360864-25360883 Msa0245580:three_prime_UTR 15.0%
!! TTTCTTAATGGAAAATATTA+AGG - chr2_2:25361772-25361791 Msa0245580:intron 15.0%
!!! AATATATTTCATTTTGAAAC+AGG + chr2_2:25362088-25362107 None:intergenic 15.0%
!!! ATTTCTAAAAAATTAATCCT+CGG + chr2_2:25362225-25362244 None:intergenic 15.0%
!! CAATAATATTACTATTTACT+GGG + chr2_2:25362287-25362306 None:intergenic 15.0%
!! AAATAAAAACAAAGCTATGA+CGG + chr2_2:25360533-25360552 None:intergenic 20.0%
!!! CTTTAATTTTTTTAACCAGA+TGG - chr2_2:25360815-25360834 Msa0245580:three_prime_UTR 20.0%
!! CAGATACAATACAAAATTTA+AGG + chr2_2:25360900-25360919 None:intergenic 20.0%
!!! CTTAAATTTTGTATTGTATC+TGG - chr2_2:25360898-25360917 Msa0245580:three_prime_UTR 20.0%
!!! AAGTTTTTATAGTAAATGAG+AGG + chr2_2:25361140-25361159 None:intergenic 20.0%
!! TTATGTATCAATAAAAGACT+CGG + chr2_2:25361242-25361261 None:intergenic 20.0%
!! ACAAATCAAACTAGTTATTA+AGG + chr2_2:25361283-25361302 None:intergenic 20.0%
!!! CACTTTCTAAAATTTCTTAA+TGG - chr2_2:25361760-25361779 Msa0245580:intron 20.0%
!! TGTTAAAAAACAAATTAAGG+TGG - chr2_2:25361896-25361915 Msa0245580:intron 20.0%
!! GTTAAAAAACAAATTAAGGT+GGG - chr2_2:25361897-25361916 Msa0245580:intron 20.0%
!! CAGGTTAAAAAATTATTATC+TGG + chr2_2:25362069-25362088 None:intergenic 20.0%
!!! TTTAGAAATTGTTTGAAACA+TGG - chr2_2:25362236-25362255 Msa0245580:intron 20.0%
!! CCAATAATATTACTATTTAC+TGG + chr2_2:25362288-25362307 None:intergenic 20.0%
!! CCAGTAAATAGTAATATTAT+TGG - chr2_2:25362285-25362304 Msa0245580:intron 20.0%
!!! ATGCATACATTAATTCTTTT+AGG + chr2_2:25362425-25362444 None:intergenic 20.0%
!! TAGATCTGAAAATAAAATGA+AGG + chr2_2:25362455-25362474 None:intergenic 20.0%
!! AAAACAGAAATACAACAAAT+GGG + chr2_2:25363189-25363208 None:intergenic 20.0%
!! TAAAACAGAAATACAACAAA+TGG + chr2_2:25363190-25363209 None:intergenic 20.0%
!! AATTACTACACCTATTAAAA+AGG + chr2_2:25363226-25363245 None:intergenic 20.0%
!!! ATTTTTTTGGATGTAAACAA+AGG - chr2_2:25363402-25363421 Msa0245580:intron 20.0%
! AATAAAAACAAAGCTATGAC+GGG + chr2_2:25360532-25360551 None:intergenic 25.0%
!!! TTCTTGTTTTTGTCTAACTT+CGG - chr2_2:25360614-25360633 Msa0245580:three_prime_UTR 25.0%
!!! TATGATAGTTTTGATGCTAT+GGG - chr2_2:25360708-25360727 Msa0245580:three_prime_UTR 25.0%
! AAATTAAAGATTGGTACAGA+AGG + chr2_2:25360804-25360823 None:intergenic 25.0%
! AAATAGGAAGCAAAACAAAA+CGG + chr2_2:25360956-25360975 None:intergenic 25.0%
! AAAAGTAGAAATCGAACTTA+TGG + chr2_2:25361029-25361048 None:intergenic 25.0%
!!! ACTAGTTTGATTTGTGTTTT+AGG - chr2_2:25361288-25361307 Msa0245580:CDS 25.0%
! TTACATCAAAATGTTCGTAT+TGG - chr2_2:25361677-25361696 Msa0245580:CDS 25.0%
!! GAAAGTGTACAAAAATTAGA+AGG + chr2_2:25361747-25361766 None:intergenic 25.0%
! ATGGGAATCTTAAATTTCTA+TGG + chr2_2:25361990-25362009 None:intergenic 25.0%
! TCAAAATCTTCAAGATTGAA+AGG + chr2_2:25362187-25362206 None:intergenic 25.0%
!!! TTTTAGGACAATAAATCAGT+AGG + chr2_2:25362409-25362428 None:intergenic 25.0%
! AAACAGAAATACAACAAATG+GGG + chr2_2:25363188-25363207 None:intergenic 25.0%
!!! TTCTGTTTTAAAACCTTGTT+TGG - chr2_2:25363200-25363219 Msa0245580:intron 25.0%
!! CAAAAACTTGCTTGTTAAAA+AGG + chr2_2:25363343-25363362 None:intergenic 25.0%
!!! CTGAAGTAGTTTGATTTTTT+TGG - chr2_2:25363389-25363408 Msa0245580:intron 25.0%
! GCTCTTTAATTAAATGAACA+TGG - chr2_2:25363560-25363579 Msa0245580:intron 25.0%
!! ATGTGTTGATCATTTCAATT+GGG + chr2_2:25363623-25363642 None:intergenic 25.0%
!! AATGTGTTGATCATTTCAAT+TGG + chr2_2:25363624-25363643 None:intergenic 25.0%
!! TGTCTAATATCTTTTCTCAA+TGG + chr2_2:25363675-25363694 None:intergenic 25.0%
ATAAAAACAAAGCTATGACG+GGG + chr2_2:25360531-25360550 None:intergenic 30.0%
!!! TCTTGTTTTTGTCTAACTTC+GGG - chr2_2:25360615-25360634 Msa0245580:three_prime_UTR 30.0%
!!! CTATGATAGTTTTGATGCTA+TGG - chr2_2:25360707-25360726 Msa0245580:three_prime_UTR 30.0%
AATAGGAAGCAAAACAAAAC+GGG + chr2_2:25360955-25360974 None:intergenic 30.0%
! GTTTTGCTTCCTATTTATCA+CGG - chr2_2:25360960-25360979 Msa0245580:three_prime_UTR 30.0%
AAGAAACAACCGTGATAAAT+AGG + chr2_2:25360972-25360991 None:intergenic 30.0%
ACAGAAACAAGAACAATCTT+GGG + chr2_2:25361064-25361083 None:intergenic 30.0%
TACAGAAACAAGAACAATCT+TGG + chr2_2:25361065-25361084 None:intergenic 30.0%
AGGATATACTACGTAGAAAA+AGG + chr2_2:25361120-25361139 None:intergenic 30.0%
AAAGTCATACAACATTAGCA+AGG + chr2_2:25361181-25361200 None:intergenic 30.0%
GGAATAGTTCCATTTATCAA+GGG - chr2_2:25361342-25361361 Msa0245580:CDS 30.0%
!! ACGAACATTTTGATGTAATC+TGG + chr2_2:25361675-25361694 None:intergenic 30.0%
TTGTGTAGTAAGGATAAAAG+TGG - chr2_2:25361923-25361942 Msa0245580:intron 30.0%
!!! GATCTTTATTTTACAAACCG+AGG - chr2_2:25362205-25362224 Msa0245580:intron 30.0%
TAGTACAAGACTGTGTTTAA+GGG + chr2_2:25362314-25362333 None:intergenic 30.0%
ATAGTACAAGACTGTGTTTA+AGG + chr2_2:25362315-25362334 None:intergenic 30.0%
! AAACACAGTCTTGTACTATT+AGG - chr2_2:25362316-25362335 Msa0245580:intron 30.0%
! AGTCTTGTACTATTAGGATT+AGG - chr2_2:25362322-25362341 Msa0245580:intron 30.0%
! GTTTTATGTCAACGTTGATA+AGG - chr2_2:25362628-25362647 Msa0245580:CDS 30.0%
GTTAACAAAAATGACACTCT+GGG + chr2_2:25362705-25362724 None:intergenic 30.0%
TGTTAACAAAAATGACACTC+TGG + chr2_2:25362706-25362725 None:intergenic 30.0%
!! TGATAATGTCCCTAGTATTT+TGG + chr2_2:25362808-25362827 None:intergenic 30.0%
TTTCTTCCATCATTACTTAG+AGG - chr2_2:25363114-25363133 Msa0245580:intron 30.0%
TTCTTCCATCATTACTTAGA+GGG - chr2_2:25363115-25363134 Msa0245580:intron 30.0%
ATTGCAAGTACTTATCACTT+AGG + chr2_2:25363274-25363293 None:intergenic 30.0%
GAATGCATTATTGTCTGAAT+TGG - chr2_2:25363297-25363316 Msa0245580:intron 30.0%
AATAAACAAACACATCCAAC+GGG + chr2_2:25363441-25363460 None:intergenic 30.0%
CAATAAACAAACACATCCAA+CGG + chr2_2:25363442-25363461 None:intergenic 30.0%
GTTGGATGTGTTTGTTTATT+GGG - chr2_2:25363441-25363460 Msa0245580:CDS 30.0%
TTGGATGTGTTTGTTTATTG+GGG - chr2_2:25363442-25363461 Msa0245580:CDS 30.0%
GTCAAAGGTTACATAAGTTA+TGG - chr2_2:25363484-25363503 Msa0245580:CDS 30.0%
AATAATATTGTGCAGCAATC+AGG + chr2_2:25363538-25363557 None:intergenic 30.0%
!! TAGTAGACTGTTTCTATTGA+AGG - chr2_2:25363646-25363665 Msa0245580:intron 30.0%
CTTCTTCACATCTTATGATG+AGG - chr2_2:25360683-25360702 Msa0245580:three_prime_UTR 35.0%
GGTATATTTCACAGTATGGA+AGG + chr2_2:25360934-25360953 None:intergenic 35.0%
AACGGGTATATTTCACAGTA+TGG + chr2_2:25360938-25360957 None:intergenic 35.0%
GGGAATAGTTCCATTTATCA+AGG - chr2_2:25361341-25361360 Msa0245580:CDS 35.0%
ACATCAAGACCCTTGATAAA+TGG + chr2_2:25361354-25361373 None:intergenic 35.0%
!!! TTCTCGTGGTTTTAAAGATC+AGG - chr2_2:25361719-25361738 Msa0245580:intron 35.0%
GTACTAGTAATCACATTCCT+AGG - chr2_2:25361794-25361813 Msa0245580:intron 35.0%
GATAAGAACAATGTTCCTAG+AGG + chr2_2:25361842-25361861 None:intergenic 35.0%
TAAGTTCAAGCTCGAGAAAT+GGG + chr2_2:25362008-25362027 None:intergenic 35.0%
TCGGTAGCAAAACTAAAATG+TGG - chr2_2:25362152-25362171 Msa0245580:intron 35.0%
AAATCTTCAAGATAGCTGAG+AGG + chr2_2:25362374-25362393 None:intergenic 35.0%
! TTGCCATCTAAGATTCAAGT+CGG - chr2_2:25362491-25362510 Msa0245580:CDS 35.0%
! TTATGTCAACGTTGATAAGG+AGG - chr2_2:25362631-25362650 Msa0245580:intron 35.0%
CTGTGATCTTTATGAGACTT+TGG - chr2_2:25362673-25362692 Msa0245580:CDS 35.0%
! TTTTGTTAACACCAGACGAA+AGG - chr2_2:25362715-25362734 Msa0245580:CDS 35.0%
AGACATGGACCAAAATACTA+GGG - chr2_2:25362796-25362815 Msa0245580:CDS 35.0%
ATCGGTAGTTATCAAAACAC+GGG + chr2_2:25362855-25362874 None:intergenic 35.0%
GATCGGTAGTTATCAAAACA+CGG + chr2_2:25362856-25362875 None:intergenic 35.0%
! TTTGATAACTACCGATCTTC+TGG - chr2_2:25362859-25362878 Msa0245580:CDS 35.0%
TCAACACATTGTAGAACTTC+TGG + chr2_2:25363048-25363067 None:intergenic 35.0%
!! GTTCTACAATGTGTTGATTG+AGG - chr2_2:25363051-25363070 Msa0245580:intron 35.0%
AACTTCCCTCTAAGTAATGA+TGG + chr2_2:25363123-25363142 None:intergenic 35.0%
ATCATTACTTAGAGGGAAGT+TGG - chr2_2:25363122-25363141 Msa0245580:intron 35.0%
! AAGTTGGTTTCTGAGTTAAG+TGG - chr2_2:25363138-25363157 Msa0245580:intron 35.0%
TACAACAAATGGGGCAAAAA+GGG + chr2_2:25363179-25363198 None:intergenic 35.0%
ATACAACAAATGGGGCAAAA+AGG + chr2_2:25363180-25363199 None:intergenic 35.0%
CCTATTAAAAAGGCCAAACA+AGG + chr2_2:25363216-25363235 None:intergenic 35.0%
!! CCTTGTTTGGCCTTTTTAAT+AGG - chr2_2:25363213-25363232 Msa0245580:intron 35.0%
TATCACTTAGGATGTTCAGA+CGG + chr2_2:25363262-25363281 None:intergenic 35.0%
AGTGATAAGTACTTGCAATG+TGG - chr2_2:25363275-25363294 Msa0245580:intron 35.0%
CGTTGGATGTGTTTGTTTAT+TGG - chr2_2:25363440-25363459 Msa0245580:CDS 35.0%
TGGATGTGTTTGTTTATTGG+GGG - chr2_2:25363443-25363462 Msa0245580:CDS 35.0%
GTTGATCATTTCAATTGGGA+TGG + chr2_2:25363619-25363638 None:intergenic 35.0%
!! TAGACTGTTTCTATTGAAGG+CGG - chr2_2:25363649-25363668 Msa0245580:intron 35.0%
! GATAATTAGTGTCGAATAGC+AGG - chr2_2:25363725-25363744 Msa0245580:CDS 35.0%
TGAGTCTGTAGATGCATTGA+AGG + chr2_2:25360647-25360666 None:intergenic 40.0%
TTGCAAGAGAATCTTCTCAG+AGG - chr2_2:25360732-25360751 Msa0245580:three_prime_UTR 40.0%
AGCACGAAAAAAATCCCATC+TGG + chr2_2:25360833-25360852 None:intergenic 40.0%
ATGTTATTGTTGGGCTCTGT+TGG - chr2_2:25360873-25360892 Msa0245580:three_prime_UTR 40.0%
GTCATACAACATTAGCAAGG+TGG + chr2_2:25361178-25361197 None:intergenic 40.0%
GGGTCTTGATGTTATACAAC+AGG - chr2_2:25361362-25361381 Msa0245580:CDS 40.0%
TGGAGATTATCTAGGTGTGA+AGG - chr2_2:25361527-25361546 Msa0245580:CDS 40.0%
CAAAATGTTCGTATTGGACG+AGG - chr2_2:25361683-25361702 Msa0245580:CDS 40.0%
!! ATTCCTAGGTATGCTGTTTC+TGG - chr2_2:25361808-25361827 Msa0245580:intron 40.0%
!! TTCCTAGGTATGCTGTTTCT+GGG - chr2_2:25361809-25361828 Msa0245580:intron 40.0%
AGGTGGGAAATTGTGTAGTA+AGG - chr2_2:25361913-25361932 Msa0245580:intron 40.0%
CTAAGTTCAAGCTCGAGAAA+TGG + chr2_2:25362009-25362028 None:intergenic 40.0%
TCGAGCTTGAACTTAGAGTA+TGG - chr2_2:25362013-25362032 Msa0245580:intron 40.0%
! CAGTAGTAGGGTTTGTTTCA+AGG + chr2_2:25362132-25362151 None:intergenic 40.0%
GAAACAAACCCTACTACTGT+CGG - chr2_2:25362133-25362152 Msa0245580:intron 40.0%
! TTTTGCTACCGACAGTAGTA+GGG + chr2_2:25362144-25362163 None:intergenic 40.0%
! ACTCCGACTTGAATCTTAGA+TGG + chr2_2:25362497-25362516 None:intergenic 40.0%
AGTTCATGAACAAACCTGTG+AGG - chr2_2:25362561-25362580 Msa0245580:CDS 40.0%
! TCAACGTTGATAAGGAGGAA+TGG - chr2_2:25362636-25362655 Msa0245580:intron 40.0%
GAGACATGGACCAAAATACT+AGG - chr2_2:25362795-25362814 Msa0245580:CDS 40.0%
TGATGTTCAGCAAGTGTCTT+TGG - chr2_2:25362892-25362911 Msa0245580:intron 40.0%
ATACGATGGAGATAGTTCTC+AGG + chr2_2:25362944-25362963 None:intergenic 40.0%
GAGAACTATCTCCATCGTAT+TGG - chr2_2:25362944-25362963 Msa0245580:intron 40.0%
AGAAGTTCAGCAACATTGGA+AGG + chr2_2:25363082-25363101 None:intergenic 40.0%
TCACAGAAGTTCAGCAACAT+TGG + chr2_2:25363086-25363105 None:intergenic 40.0%
ACAACAAATGGGGCAAAAAG+GGG + chr2_2:25363178-25363197 None:intergenic 40.0%
! TGTAAACAAAGGTGTATCGC+TGG - chr2_2:25363413-25363432 Msa0245580:intron 40.0%
GGATGTGTTTGTTTATTGGG+GGG - chr2_2:25363444-25363463 Msa0245580:CDS 40.0%
! CATTACGAACATGCTACTTG+AGG + chr2_2:25363586-25363605 None:intergenic 40.0%
TCCTTTACTTCCTGCAGTCA+TGG - chr2_2:25360431-25360450 Msa0245580:three_prime_UTR 45.0%
!! CTCAGAGGCATTTACGCATA+TGG - chr2_2:25360747-25360766 Msa0245580:three_prime_UTR 45.0%
AGCCATCAGCTATTCAGCAA+CGG - chr2_2:25361319-25361338 Msa0245580:CDS 45.0%
GTTATACAACAGGCTCAGTC+TGG - chr2_2:25361372-25361391 Msa0245580:CDS 45.0%
CGTGCACTTGGAGATTATCT+AGG - chr2_2:25361519-25361538 Msa0245580:CDS 45.0%
GATCAGCGCATTCTATCAAG+CGG - chr2_2:25361585-25361604 Msa0245580:CDS 45.0%
AGCGGTGTTCATGTTGTTGT+TGG - chr2_2:25361603-25361622 Msa0245580:CDS 45.0%
CATGTCAAATACACGTCCAG+GGG + chr2_2:25361631-25361650 None:intergenic 45.0%
GCATGTCAAATACACGTCCA+GGG + chr2_2:25361632-25361651 None:intergenic 45.0%
AGCATGTCAAATACACGTCC+AGG + chr2_2:25361633-25361652 None:intergenic 45.0%
TTCCCAGAAACAGCATACCT+AGG + chr2_2:25361814-25361833 None:intergenic 45.0%
! TTTCTGGGAATTGCACCTCT+AGG - chr2_2:25361824-25361843 Msa0245580:intron 45.0%
! GTTTTGCTACCGACAGTAGT+AGG + chr2_2:25362145-25362164 None:intergenic 45.0%
GAATCTTAGATGGCAACAGC+TGG + chr2_2:25362487-25362506 None:intergenic 45.0%
AAACTGCTTGATACCCTCCA+AGG + chr2_2:25362612-25362631 None:intergenic 45.0%
ATCATGCGTGAATTCCGTTC+TGG - chr2_2:25362824-25362843 Msa0245580:CDS 45.0%
AAACACGGGAAGATCCAGAA+CGG + chr2_2:25362841-25362860 None:intergenic 45.0%
AATACCACGAGCCAGAAGAT+CGG + chr2_2:25362873-25362892 None:intergenic 45.0%
GATAGTTCTCAGGCTGTGTT+GGG + chr2_2:25362934-25362953 None:intergenic 45.0%
AGATAGTTCTCAGGCTGTGT+TGG + chr2_2:25362935-25362954 None:intergenic 45.0%
TTCCACTACGACCAATACGA+TGG + chr2_2:25362958-25362977 None:intergenic 45.0%
! ATCGTATTGGTCGTAGTGGA+AGG - chr2_2:25362957-25362976 Msa0245580:intron 45.0%
! ATTGGTCGTAGTGGAAGGTT+TGG - chr2_2:25362962-25362981 Msa0245580:intron 45.0%
AGTGGAAGGTTTGGAAGGAA+AGG - chr2_2:25362971-25362990 Msa0245580:intron 45.0%
!! AGATGACTCGATTCGGTCAA+AGG - chr2_2:25363469-25363488 Msa0245580:CDS 45.0%
GCCATGACTGCAGGAAGTAA+AGG + chr2_2:25360435-25360454 None:intergenic 50.0%
TTACTTCCTGCAGTCATGGC+AGG - chr2_2:25360435-25360454 Msa0245580:three_prime_UTR 50.0%
GCATCAAACTGTGAACCCTC+TGG + chr2_2:25360468-25360487 None:intergenic 50.0%
CAGACTCACAACTGATGCAC+AGG - chr2_2:25360659-25360678 Msa0245580:three_prime_UTR 50.0%
GCCATCAGCTATTCAGCAAC+GGG - chr2_2:25361320-25361339 Msa0245580:CDS 50.0%
CCATCAGCTATTCAGCAACG+GGG - chr2_2:25361321-25361340 Msa0245580:CDS 50.0%
! AACAGGCTCAGTCTGGAACA+GGG - chr2_2:25361379-25361398 Msa0245580:CDS 50.0%
AAGACTGCAACCTTCTGCTC+TGG - chr2_2:25361402-25361421 Msa0245580:CDS 50.0%
AGACTGCAACCTTCTGCTCT+GGG - chr2_2:25361403-25361422 Msa0245580:CDS 50.0%
CTGCAAAATCCCAGAGCAGA+AGG + chr2_2:25361415-25361434 None:intergenic 50.0%
CAAGACCAAGGCTTGGCATT+CGG + chr2_2:25361457-25361476 None:intergenic 50.0%
GCTTGCTCAGCAGATTGAGA+AGG - chr2_2:25361491-25361510 Msa0245580:CDS 50.0%
! GTGAAGGTGCATGCTTGTGT+CGG - chr2_2:25361543-25361562 Msa0245580:CDS 50.0%
! CATGTTGTTGTTGGTACCCC+TGG - chr2_2:25361612-25361631 Msa0245580:CDS 50.0%
GCTGATGAGATGCTTTCTCG+TGG - chr2_2:25361705-25361724 Msa0245580:CDS 50.0%
CTTGTGATCTCAAGTGCCTC+AGG + chr2_2:25362539-25362558 None:intergenic 50.0%
! CTGAGGCACTTGAGATCACA+AGG - chr2_2:25362537-25362556 Msa0245580:CDS 50.0%
CGCTTCACAAGAATCCTCAC+AGG + chr2_2:25362578-25362597 None:intergenic 50.0%
ACACCAGACGAAAGGTTGAC+TGG - chr2_2:25362723-25362742 Msa0245580:CDS 50.0%
TTGCTGAGACAGTGTGGTCA+CGG + chr2_2:25362769-25362788 None:intergenic 50.0%
CACACTGTCTCAGCAACACA+CGG - chr2_2:25362773-25362792 Msa0245580:CDS 50.0%
! CTCCATCGTATTGGTCGTAG+TGG - chr2_2:25362953-25362972 Msa0245580:intron 50.0%
! GTCGTAGTGGAAGGTTTGGA+AGG - chr2_2:25362966-25362985 Msa0245580:intron 50.0%
GGGGGATAGATGACTCGATT+CGG - chr2_2:25363462-25363481 Msa0245580:CDS 50.0%
CCCCGTTGCTGAATAGCTGA+TGG + chr2_2:25361324-25361343 None:intergenic 55.0%
! CAACAGGCTCAGTCTGGAAC+AGG - chr2_2:25361378-25361397 Msa0245580:CDS 55.0%
TGTGACCGAATGCCAAGCCT+TGG - chr2_2:25361449-25361468 Msa0245580:CDS 55.0%
CGAATGCCAAGCCTTGGTCT+TGG - chr2_2:25361455-25361474 Msa0245580:CDS 55.0%
TAGGTGCCAAGACCAAGGCT+TGG + chr2_2:25361464-25361483 None:intergenic 55.0%
ACGAGTAGGTGCCAAGACCA+AGG + chr2_2:25361469-25361488 None:intergenic 55.0%
TGCTGAGCAAGCTCACGAGT+AGG + chr2_2:25361483-25361502 None:intergenic 55.0%
GAGAAGGTCATGCGTGCACT+TGG - chr2_2:25361507-25361526 Msa0245580:CDS 55.0%
AAGGTGCATGCTTGTGTCGG+AGG - chr2_2:25361546-25361565 Msa0245580:CDS 55.0%
! GCGCTGATCTTCACGAACAC+TGG + chr2_2:25361574-25361593 None:intergenic 55.0%
CTCTGCTACAATGCCTCCTG+AGG - chr2_2:25362520-25362539 Msa0245580:CDS 55.0%
GTGATCTCAAGTGCCTCAGG+AGG + chr2_2:25362536-25362555 None:intergenic 55.0%
GAAGCGTGATGAGCTCACCT+TGG - chr2_2:25362592-25362611 Msa0245580:CDS 55.0%
CGTGATGAGCTCACCTTGGA+GGG - chr2_2:25362596-25362615 Msa0245580:CDS 55.0%
CAGCCAGTCAACCTTTCGTC+TGG + chr2_2:25362729-25362748 None:intergenic 55.0%
! CGTGTGTTGCTGAGACAGTG+TGG + chr2_2:25362775-25362794 None:intergenic 55.0%
CTCAGCAACACACGGAGACA+TGG - chr2_2:25362781-25362800 Msa0245580:CDS 55.0%
!! ACTACCGATCTTCTGGCTCG+TGG - chr2_2:25362866-25362885 Msa0245580:CDS 55.0%
! ATGGCAGGCGTTGCACCAGA+GGG - chr2_2:25360450-25360469 Msa0245580:three_prime_UTR 60.0%
GCGTGATGAGCTCACCTTGG+AGG - chr2_2:25362595-25362614 Msa0245580:CDS 60.0%
GGTGTATCGCTGGTACCCGT+TGG - chr2_2:25363423-25363442 Msa0245580:intron 60.0%
GCAACGCCTGCCATGACTGC+AGG + chr2_2:25360444-25360463 None:intergenic 65.0%
! CATGGCAGGCGTTGCACCAG+AGG - chr2_2:25360449-25360468 Msa0245580:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 25360427 25363769 25360427 ID=Msa0245580;Name=Msa0245580
chr2_2 mRNA 25360427 25363769 25360427 ID=Msa0245580-mRNA-1;Parent=Msa0245580;Name=Msa0245580-mRNA-1;_AED=0.05;_eAED=0.05;_QI=23|0.75|0.6|1|0.75|0.6|5|664|411
chr2_2 exon 25360427 25361726 25360427 ID=Msa0245580-mRNA-1:exon:9562;Parent=Msa0245580-mRNA-1
chr2_2 exon 25362456 25362647 25362456 ID=Msa0245580-mRNA-1:exon:9561;Parent=Msa0245580-mRNA-1
chr2_2 exon 25362654 25362886 25362654 ID=Msa0245580-mRNA-1:exon:9560;Parent=Msa0245580-mRNA-1
chr2_2 exon 25363428 25363534 25363428 ID=Msa0245580-mRNA-1:exon:9559;Parent=Msa0245580-mRNA-1
chr2_2 exon 25363679 25363769 25363679 ID=Msa0245580-mRNA-1:exon:9558;Parent=Msa0245580-mRNA-1
chr2_2 five_prime_UTR 25363747 25363769 25363747 ID=Msa0245580-mRNA-1:five_prime_utr;Parent=Msa0245580-mRNA-1
chr2_2 CDS 25363679 25363746 25363679 ID=Msa0245580-mRNA-1:cds;Parent=Msa0245580-mRNA-1
chr2_2 CDS 25363428 25363534 25363428 ID=Msa0245580-mRNA-1:cds;Parent=Msa0245580-mRNA-1
chr2_2 CDS 25362654 25362886 25362654 ID=Msa0245580-mRNA-1:cds;Parent=Msa0245580-mRNA-1
chr2_2 CDS 25362456 25362647 25362456 ID=Msa0245580-mRNA-1:cds;Parent=Msa0245580-mRNA-1
chr2_2 CDS 25361091 25361726 25361091 ID=Msa0245580-mRNA-1:cds;Parent=Msa0245580-mRNA-1
chr2_2 three_prime_UTR 25360427 25361090 25360427 ID=Msa0245580-mRNA-1:three_prime_utr;Parent=Msa0245580-mRNA-1
Gene Sequence

>Msa0245580

ATGGCAGGCGTTGCACCAGAGGGTTCACAGTTTGATGCTAAGCAGTTTGATTCAAAAATGAATGAGTTACTCACAACTGATGCACAGGACTTCTTCACATCTTATGATGAGGTCTATGATAGTTTTGATGCTATGGGCTTGCAAGAGAATCTTCTCAGAGGCATTTACGCATATGGTTTTGAGAAGCCATCAGCTATTCAGCAACGGGGAATAGTTCCATTTATCAAGGGTCTTGATGTTATACAACAGGCTCAGTCTGGAACAGGGAAGACTGCAACCTTCTGCTCTGGGATTTTGCAGCAACTTGACTACAGTGTGACCGAATGCCAAGCCTTGGTCTTGGCACCTACTCGTGAGCTTGCTCAGCAGATTGAGAAGGTCATGCGTGCACTTGGAGATTATCTAGGTGTGCATGCTTGTGTCGGAGGAACCAGTGTTCGTGAAGATCAGCGCATTCTATCAAGCGGTGTTCATGTTGTTGTTGGTACCCCTGGACGTGTATTTGACATGCTGCGCAGACAGTCGCTTCGACCAGATTACATCAAAATGTTCGTATTGGACGAGGCTGATGAGATGCTTTCTCGTGGTTTTAAAGATCAGATCTATGATATATTCCAGCTGTTGCCATCTAAGATTCAAGTCGGAGTTTTCTCTGCTACAATGCCTCCTGAGGCACTTGAGATCACAAGGAAGTTCATGAACAAACCTGTGAGGATTCTTGTGAAGCGTGATGAGCTCACCTTGGAGGGTATCAAGCAGTTTTATGTCAACGTTGATAAGGAGGAATGGAAGCTCGACACACTCTGTGATCTTTATGAGACTTTGGCTATCACCCAGAGTGTCATTTTTGTTAACACCAGACGAAAGGTTGACTGGCTGACTGACAAGATGAGAAGCCGTGACCACACTGTCTCAGCAACACACGGAGACATGGACCAAAATACTAGGGACATTATCATGCGTGAATTCCGTTCTGGATCTTCCCGTGTTTTGATAACTACCGATCTTCTGGCTCGTGGTATTGATGTTCAGCAAGTGTCTTTGGTTATAAATTATGATCTCCCAACACAGCCTGAGAACTATCTCCATCGTATTGGTCGTAGTGGAAGGTTTGGAAGGAAAGGTGTTGCTATTAATTTCGTCACAACTGATGACGAAAGAATGCTGAGTGACATCCAGAAGTTCTACAATGTGTTGATTGAGGAGCTGCCTTCCAATGTTGCTGAACTTCTGTGA
Protein sequence

>Msa0245580

MAGVAPEGSQFDAKQFDSKMNELLTTDAQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFIKGLDVIQQAQSGTGKTATFCSGILQQLDYSVTECQALVLAPTRELAQQIEKVMRALGDYLGVHACVGGTSVREDQRILSSGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPSKIQVGVFSATMPPEALEITRKFMNKPVRILVKRDELTLEGIKQFYVNVDKEEWKLDTLCDLYETLAITQSVIFVNTRRKVDWLTDKMRSRDHTVSATHGDMDQNTRDIIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTQPENYLHRIGRSGRFGRKGVAINFVTTDDERMLSDIQKFYNVLIEELPSNVAELL*