Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1453720 | sp|A4QLL3|PETG_LOBMA | 86.111 | 36 | 5 | 0 | 28 | 63 | 1 | 36 | 5.02e-15 | 64.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1453720 | A0A396GH67 | 72.131 | 61 | 17 | 0 | 4 | 64 | 11 | 71 | 2.13e-22 | 90.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1438250 | Msa1453720 | 0.804249 | 2.365175e-49 | -8.615850e-47 |
| Msa0627410 | Msa1453720 | 0.817681 | 2.906249e-52 | -8.615850e-47 |
| Msa0649960 | Msa1453720 | 0.804369 | 2.233338e-49 | -8.615850e-47 |
| Msa0736000 | Msa1453720 | 0.801304 | 9.591160e-49 | -8.615850e-47 |
| Msa0777790 | Msa1453720 | 0.805128 | 1.550193e-49 | -8.615850e-47 |
| Msa0811080 | Msa1453720 | 0.804609 | 1.990100e-49 | -8.615850e-47 |
| Msa0153910 | Msa1453720 | 0.812459 | 4.195582e-51 | -8.615850e-47 |
| Msa0456200 | Msa1453720 | 0.809910 | 1.498136e-50 | -8.615850e-47 |
| Msa0353190 | Msa1453720 | 0.804811 | 1.805763e-49 | -8.615850e-47 |
| Msa1136090 | Msa1453720 | 0.823805 | 1.136673e-53 | -8.615850e-47 |
| Msa1160670 | Msa1453720 | 0.801067 | 1.072434e-48 | -8.615850e-47 |
| Msa1176760 | Msa1453720 | 0.808114 | 3.630204e-50 | -8.615850e-47 |
| Msa0558640 | Msa1453720 | 0.837266 | 5.750977e-57 | -8.615850e-47 |
| Msa0885280 | Msa1453720 | 0.835427 | 1.689041e-56 | -8.615850e-47 |
| Msa0885290 | Msa1453720 | 0.812048 | 5.158405e-51 | -8.615850e-47 |
| Msa0959770 | Msa1453720 | 0.810186 | 1.306066e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1453720 | MtrunA17_Chr8g0355631 | 72.131 | 61 | 17 | 0 | 4 | 64 | 11 | 71 | 4.11e-26 | 90.5 |
| Msa1453720 | MtrunA17_Chr4g0015761 | 72.131 | 61 | 17 | 0 | 4 | 64 | 11 | 71 | 5.12e-26 | 90.1 |
| Msa1453720 | MtrunA17_CPg0492811 | 72.131 | 61 | 17 | 0 | 4 | 64 | 11 | 71 | 5.12e-26 | 90.1 |
| Msa1453720 | MtrunA17_Chr4g0016861 | 85.714 | 35 | 5 | 0 | 28 | 62 | 1 | 35 | 3.32e-15 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1453720 | ATCG00600.1 | 83.333 | 36 | 6 | 0 | 28 | 63 | 1 | 36 | 9.99e-15 | 61.2 |
Find 15 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATTGCCAATTCCTATTTA+AGG | 0.126954 | tig0034889:-7748 | Msa1453720:CDS |
| ATGATTGAAGTTTCTCTGTT+TGG | 0.294603 | tig0034889:-7684 | Msa1453720:CDS |
| GGTAATATCTATCCTTAAAT+AGG | 0.297194 | tig0034889:+7736 | Msa1453720:intergenic |
| AATAATTCAGCCAAAGTAAT+AGG | 0.317142 | tig0034889:+7642 | Msa1453720:intergenic |
| AAACTTCAATCATTTGAGTT+TGG | 0.328054 | tig0034889:+7694 | Msa1453720:intergenic |
| TCTCTGTTTGGAATCATGTT+AGG | 0.339530 | tig0034889:-7672 | Msa1453720:CDS |
| ATCTATCCTTAAATAGGAAT+TGG | 0.360922 | tig0034889:+7742 | Msa1453720:intergenic |
| AGGTCTAGTTCCTATTACTT+TGG | 0.408917 | tig0034889:-7652 | Msa1453720:CDS |
| ATCATTTGAGTTTGGTTGAA+GGG | 0.457044 | tig0034889:+7702 | Msa1453720:intergenic |
| AATTGGCAATTGACATGATA+AGG | 0.468100 | tig0034889:+7759 | Msa1453720:intergenic |
| AATCATTTGAGTTTGGTTGA+AGG | 0.519725 | tig0034889:+7701 | Msa1453720:intergenic |
| TTTGGCTGAATTATTTGTAA+CGG | 0.545379 | tig0034889:-7634 | Msa1453720:CDS |
| ATACCGACATGGTGATCAGT+TGG | 0.579917 | tig0034889:-7601 | Msa1453720:CDS |
| GCATATTTACGATACCGACA+TGG | 0.642520 | tig0034889:-7612 | Msa1453720:CDS |
| TATCCAACTGATCACCATGT+CGG | 0.675750 | tig0034889:+7598 | Msa1453710:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAAAAATTTAGTTACAT+AGG | + | tig0034889:7632-7651 | Msa0958720:intergenic | 10.0% |
| !!! | TCTATTTTTGAGATAAAAAT+AGG | + | tig0034889:7732-7751 | Msa1453720:intergenic | 15.0% |
| !! | AAAAATTTAGTTACATAGGT+AGG | + | tig0034889:7628-7647 | Msa0958720:intergenic | 20.0% |
| !! | GATTCATGAATCAATTATTA+CGG | + | tig0034889:7663-7682 | Msa0958720:intergenic | 20.0% |
| !! | TATCTCAAAAATAGAAACTT+AGG | - | tig0034889:7737-7756 | Msa1453720:CDS | 20.0% |
| !!! | TTATTAGTCTGAATAAGATA+TGG | - | tig0034889:7877-7896 | Msa1453720:intron | 20.0% |
| !!! | TTGAGATGAATTGTAAATTT+TGG | - | tig0034889:7918-7937 | Msa1453720:intron | 20.0% |
| ! | TTTCAATTGTATCTTTCAAG+TGG | - | tig0034889:7689-7708 | Msa1453720:CDS | 25.0% |
| !!! | ATCTTTCAAGTGGTATTTTT+TGG | - | tig0034889:7699-7718 | Msa1453720:CDS | 25.0% |
| ! | ATCTATCCTTAAATAGGAAT+TGG | + | tig0034889:7984-8003 | Msa1453720:intergenic | 25.0% |
| ! | GGTAATATCTATCCTTAAAT+AGG | + | tig0034889:7990-8009 | Msa1453720:intergenic | 25.0% |
| !! | AAACTTCAATCATTTGAGTT+TGG | + | tig0034889:8032-8051 | Msa1453720:intergenic | 25.0% |
| ! | AATAATTCAGCCAAAGTAAT+AGG | + | tig0034889:8084-8103 | Msa1453720:intergenic | 25.0% |
| ! | TTTGGCTGAATTATTTGTAA+CGG | - | tig0034889:8089-8108 | Msa1453720:intron | 25.0% |
| CCAAAATAACTCGTTATAGT+GGG | + | tig0034889:7819-7838 | Msa1453720:intergenic | 30.0% | |
| ACCAAAATAACTCGTTATAG+TGG | + | tig0034889:7820-7839 | Msa1453720:intergenic | 30.0% | |
| AATTGGCAATTGACATGATA+AGG | + | tig0034889:7967-7986 | Msa1453720:intergenic | 30.0% | |
| CAATTGCCAATTCCTATTTA+AGG | - | tig0034889:7975-7994 | Msa1453720:intron | 30.0% | |
| ! | ATCATTTGAGTTTGGTTGAA+GGG | + | tig0034889:8024-8043 | Msa1453720:intergenic | 30.0% |
| ! | AATCATTTGAGTTTGGTTGA+AGG | + | tig0034889:8025-8044 | Msa1453720:intergenic | 30.0% |
| ATGATTGAAGTTTCTCTGTT+TGG | - | tig0034889:8039-8058 | Msa1453720:intron | 30.0% | |
| ! | CCCACTATAACGAGTTATTT+TGG | - | tig0034889:7816-7835 | Msa1453720:intron | 35.0% |
| !! | AGCAGTTTTAACAATAACCG+CGG | - | tig0034889:7848-7867 | Msa1453720:intron | 35.0% |
| TGGTTGAAGGGAAAAAAAGT+GGG | + | tig0034889:8012-8031 | Msa1453720:intergenic | 35.0% | |
| TTGGTTGAAGGGAAAAAAAG+TGG | + | tig0034889:8013-8032 | Msa1453720:intergenic | 35.0% | |
| ! | TCTCTGTTTGGAATCATGTT+AGG | - | tig0034889:8051-8070 | Msa1453720:intron | 35.0% |
| ! | AGGTCTAGTTCCTATTACTT+TGG | - | tig0034889:8071-8090 | Msa1453720:intron | 35.0% |
| !! | AACTCGTTATAGTGGGCATA+AGG | + | tig0034889:7812-7831 | Msa1453720:intergenic | 40.0% |
| !! | GCAGTTTTAACAATAACCGC+GGG | - | tig0034889:7849-7868 | Msa1453720:intron | 40.0% |
| CAGACTAATAAAAAGACCCG+CGG | + | tig0034889:7868-7887 | Msa1453720:intergenic | 40.0% | |
| GGTTGAAGGGAAAAAAAGTG+GGG | + | tig0034889:8011-8030 | Msa1453720:intergenic | 40.0% | |
| GCATATTTACGATACCGACA+TGG | - | tig0034889:8111-8130 | Msa1453720:intron | 40.0% | |
| TATCCAACTGATCACCATGT+CGG | + | tig0034889:8128-8147 | Msa1453720:intergenic | 40.0% | |
| ATACCGACATGGTGATCAGT+TGG | - | tig0034889:8122-8141 | Msa1453720:intron | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0034889 | gene | 7593 | 8152 | 7593 | ID=Msa1453720;Name=Msa1453720 |
| tig0034889 | mRNA | 7593 | 8152 | 7593 | ID=Msa1453720-mRNA-1;Parent=Msa1453720;Name=Msa1453720-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0|0|0|0.5|0|0|2|0|64 |
| tig0034889 | exon | 8149 | 8152 | 8149 | ID=Msa1453720-mRNA-1:exon:19304;Parent=Msa1453720-mRNA-1 |
| tig0034889 | exon | 7593 | 7783 | 7593 | ID=Msa1453720-mRNA-1:exon:19303;Parent=Msa1453720-mRNA-1 |
| tig0034889 | CDS | 8149 | 8152 | 8149 | ID=Msa1453720-mRNA-1:cds;Parent=Msa1453720-mRNA-1 |
| tig0034889 | CDS | 7593 | 7783 | 7593 | ID=Msa1453720-mRNA-1:cds;Parent=Msa1453720-mRNA-1 |
| Gene Sequence |
| Protein sequence |
