AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1461200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461200 MtrunA17_Chr1g0200071 99.554 448 2 0 1 448 1 448 0.0 916
Msa1461200 MtrunA17_Chr1g0200091 99.554 448 2 0 1 448 1 448 0.0 916
Msa1461200 MtrunA17_Chr1g0200121 99.330 448 3 0 1 448 1 448 0.0 915
Msa1461200 MtrunA17_Chr6g0458111 96.652 448 14 1 1 448 1 447 0.0 896
Msa1461200 MtrunA17_Chr6g0458091 96.652 448 14 1 1 448 1 447 0.0 896
Msa1461200 MtrunA17_Chr4g0005781 96.205 448 16 1 1 448 1 447 0.0 890
Msa1461200 MtrunA17_Chr1g0150331 91.353 451 10 2 1 448 1 425 0.0 825
Msa1461200 MtrunA17_Chr1g0193591 81.674 442 81 0 1 442 1 442 0.0 746
Msa1461200 MtrunA17_Chr1g0204031 82.151 437 73 3 1 437 1 432 0.0 736
Msa1461200 MtrunA17_Chr1g0156421 79.263 434 90 0 1 434 1 434 0.0 728
Msa1461200 MtrunA17_Chr8g0340771 79.733 449 89 1 1 447 1 449 0.0 723
Msa1461200 MtrunA17_Chr5g0415391 72.796 397 108 0 39 435 1 397 0.0 595
Msa1461200 MtrunA17_Chr1g0196251 69.727 403 94 2 4 405 5 380 0.0 576
Msa1461200 MtrunA17_Chr8g0336511 78.593 327 69 1 1 326 66 392 0.0 543
Msa1461200 MtrunA17_Chr2g0319341 73.952 334 85 1 102 433 1 334 0.0 516
Msa1461200 MtrunA17_Chr2g0298371 68.923 325 82 2 102 424 1 308 1.91e-161 457
Msa1461200 MtrunA17_Chr1g0210931 63.087 298 56 4 155 447 1 249 1.03e-118 347
Msa1461200 MtrunA17_Chr8g0340371 70.124 241 69 2 1 240 1 239 1.57e-118 346
Msa1461200 MtrunA17_Chr2g0318291 71.681 226 59 3 1 223 1 224 3.14e-112 329
Msa1461200 MtrunA17_Chr6g0483121 37.963 432 254 6 5 428 89 514 4.78e-97 301
Msa1461200 MtrunA17_Chr8g0392641 37.963 432 258 6 4 429 143 570 1.96e-92 290
Msa1461200 MtrunA17_Chr8g0380091 36.028 433 268 6 7 433 275 704 2.08e-91 291
Msa1461200 MtrunA17_Chr1g0204041 86.452 155 21 0 1 155 1 155 1.03e-90 272
Msa1461200 MtrunA17_Chr1g0200111 75.706 177 33 1 264 430 1 177 5.68e-87 263
Msa1461200 MtrunA17_Chr2g0318281 66.010 203 62 1 243 445 5 200 2.07e-84 258
Msa1461200 MtrunA17_Chr1g0156411 81.429 140 26 0 6 145 10 149 1.13e-78 241
Msa1461200 MtrunA17_Chr8g0340361 60.541 185 57 2 264 433 1 184 3.75e-66 210
Msa1461200 MtrunA17_Chr4g0026961 54.639 194 72 4 195 385 32 212 1.72e-58 191
Msa1461200 MtrunA17_Chr4g0026961 76.190 42 10 0 1 42 1 42 6.46e-13 67.8
Msa1461200 MtrunA17_Chr2g0298381 77.778 99 22 0 1 99 1 99 5.87e-48 159
Msa1461200 MtrunA17_Chr8g0341021 76.471 102 24 0 3 104 4 105 1.28e-47 159
Msa1461200 MtrunA17_Chr7g0259481 30.365 438 245 14 3 429 59 447 1.23e-45 164
Msa1461200 MtrunA17_Chr3g0116821 29.955 444 250 15 3 429 88 487 1.24e-40 151
Msa1461200 MtrunA17_Chr1g0211481 79.747 79 16 0 5 83 4 82 3.58e-36 129
Msa1461200 MtrunA17_Chr2g0319351 82.192 73 13 0 5 77 5 77 4.40e-35 125
Msa1461200 MtrunA17_Chr1g0200101 89.231 65 7 0 172 236 49 113 7.50e-35 125
Msa1461200 MtrunA17_Chr1g0200101 73.469 49 13 0 21 69 2 50 9.15e-17 76.3
Msa1461200 MtrunA17_Chr2g0287231 32.389 247 135 6 1 244 10 227 8.62e-31 118
Msa1461200 MtrunA17_Chr8g0341041 67.089 79 26 0 213 291 2 80 8.29e-28 108
Msa1461200 MtrunA17_Chr8g0341041 63.793 58 21 0 375 432 101 158 1.44e-17 80.1
Msa1461200 MtrunA17_Chr7g0261801 69.697 66 17 1 155 220 1 63 4.02e-24 95.9
Msa1461200 MtrunA17_Chr3g0130991 26.471 272 143 9 9 272 85 307 1.05e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1461200 AT1G07920.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT1G07940.4 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT1G07940.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT1G07940.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT1G07940.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT5G60390.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT5G60390.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT5G60390.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT1G07930.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1461200 AT1G07930.2 98.824 255 3 0 1 255 1 255 0.0 530
Msa1461200 AT1G07930.2 88.034 117 13 1 331 447 254 369 1.13e-65 215
Msa1461200 AT1G18070.1 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa1461200 AT1G18070.2 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa1461200 AT1G18070.3 36.927 436 256 6 5 424 99 531 4.09e-91 286
Msa1461200 AT5G10630.1 35.765 425 265 5 7 426 239 660 2.27e-90 288
Msa1461200 AT5G10630.5 35.765 425 265 5 7 426 240 661 2.32e-90 288
Msa1461200 AT5G10630.2 35.765 425 265 5 7 426 240 661 2.32e-90 288
Msa1461200 AT5G10630.3 35.765 425 265 5 7 426 310 731 1.32e-89 288
Msa1461200 AT5G10630.4 35.765 425 265 5 7 426 315 736 1.39e-89 288
Msa1461200 AT1G35550.1 78.641 103 22 0 335 437 1 103 1.62e-53 174
Msa1461200 AT4G02930.1 30.296 439 245 15 3 429 63 452 7.41e-44 160
Msa1461200 AT4G20360.1 30.137 438 257 10 3 429 75 474 1.70e-43 159
Msa1461200 AT4G20360.2 30.137 438 257 10 3 429 75 474 1.70e-43 159
Msa1461200 AT2G31060.3 25.000 264 160 8 9 263 61 295 3.24e-12 68.9
Msa1461200 AT5G08650.1 26.667 270 145 10 9 272 88 310 2.26e-11 66.6
Msa1461200 AT5G08650.2 26.667 270 145 10 9 272 88 310 2.33e-11 66.2
Msa1461200 AT2G31060.2 24.615 260 162 7 9 263 61 291 4.67e-11 65.5

Find 188 sgRNAs with CRISPR-Local

Find 263 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACCATTCTTCAAGAATT+TGG 0.066665 tig0041342:-15284 None:intergenic
TAATTCTATCAAATTGTTTC+TGG 0.156599 tig0041342:+15906 Msa1461200:three_prime_UTR
ATCATAGTTATTACAATAAA+TGG 0.259832 tig0041342:+15530 Msa1461200:three_prime_UTR
TGCTCTACCACTGAGCTATA+AGG 0.259926 tig0041342:-13740 None:intergenic
GCTCTCCTTGCTTTCACTCT+TGG 0.289982 tig0041342:+14440 Msa1461200:CDS
CAAACTATTACAAATAAAAT+AGG 0.299324 tig0041342:+15701 Msa1461200:three_prime_UTR
GTCTACAAGATTGGAGGAAT+TGG 0.315206 tig0041342:+14869 Msa1461200:CDS
GCCTCTTAGGCTCATTGATT+TGG 0.315866 tig0041342:-14811 None:intergenic
CTTAGTAAACTCAATGAGTT+TGG 0.317766 tig0041342:+15795 Msa1461200:three_prime_UTR
TTATTACAATAAATGGTCTT+TGG 0.319176 tig0041342:+15537 Msa1461200:three_prime_UTR
AATTTCTTATTGCTTGAGTT+TGG 0.324120 tig0041342:-15608 None:intergenic
TGTCAAGAATGTTGCTGTTA+AGG 0.325240 tig0041342:+15045 Msa1461200:CDS
TGGACAATGAACAAATAAAT+AGG 0.329863 tig0041342:+15815 Msa1461200:three_prime_UTR
TCACCATTCTTCAAGAATTT+GGG 0.331757 tig0041342:-15283 None:intergenic
ATAGCCAACCTTCTTCAAAT+AGG 0.332380 tig0041342:-14675 None:intergenic
TTGCGCAGCTTTCCTCTTCA+AGG 0.350006 tig0041342:+12746 Msa1461200:five_prime_UTR
ATTCCATTTGTTCCCATCTC+TGG 0.368209 tig0041342:+14710 Msa1461200:CDS
CTTCTTCTTCAATGCTGACT+TGG 0.371330 tig0041342:-15470 None:intergenic
CTCCTTGGCTGGGTCATCCT+TGG 0.371873 tig0041342:-15098 None:intergenic
ATATCTACCCTTGGAGTACT+TGG 0.380807 tig0041342:-14627 None:intergenic
ACTAAGATTGATAGGCGTTC+TGG 0.382761 tig0041342:+15241 Msa1461200:CDS
AATAAATGGTCTTTGGTCCT+TGG 0.384333 tig0041342:+15544 Msa1461200:three_prime_UTR
GCTCTAACAGAGGCCCTTCC+TGG 0.389893 tig0041342:+15004 Msa1461200:CDS
AAGCTAGCAGCCTCCTTGGC+TGG 0.398839 tig0041342:-15109 None:intergenic
GTAGCGGTCCATCAATTTCA+TGG 0.402233 tig0041342:-15760 None:intergenic
GGATCCCAGTGGAGGACTTA+AGG 0.415493 tig0041342:+15444 Msa1461200:CDS
GCTCTACCACTGAGCTATAA+GGG 0.416492 tig0041342:-13739 None:intergenic
GCAAAAGTGACAACCATTCC+AGG 0.418006 tig0041342:-14932 None:intergenic
CTCATCATTGATTCCACTAC+TGG 0.419293 tig0041342:+14371 Msa1461200:CDS
GAGGTGTGGCAGTCAAGAAC+AGG 0.420700 tig0041342:-15187 None:intergenic
ATGAATCACCCTGGCCAGAT+TGG 0.425183 tig0041342:+15151 Msa1461200:CDS
CCTGTGGGGCGTGTCGAGAC+TGG 0.431830 tig0041342:+14899 Msa1461200:CDS
CACCCTGGCCAGATTGGAAA+TGG 0.432985 tig0041342:+15157 Msa1461200:CDS
GCCCCTTCGACTACCACTTC+AGG 0.433104 tig0041342:+14844 Msa1461200:CDS
GTCATCGGCCATGTTGACTC+TGG 0.440627 tig0041342:+14065 Msa1461200:CDS
AGGACTAATGGGTAAGGAAA+AGG 0.440646 tig0041342:+14025 Msa1461200:exon
ACATTCTCATCCTTGCGGCT+AGG 0.443267 tig0041342:+12719 Msa1461200:five_prime_UTR
CATTCTCATCCTTGCGGCTA+GGG 0.444010 tig0041342:+12720 Msa1461200:five_prime_UTR
AGCGTTCATTCAAGTATGCC+TGG 0.444655 tig0041342:+14183 Msa1461200:CDS
GGGCATAGCCATTTCCAATC+TGG 0.448022 tig0041342:-15165 None:intergenic
AAACCAGAGATGGGAACAAA+TGG 0.449170 tig0041342:-14713 None:intergenic
AAAATAGGTAACATAGATTC+CGG 0.454200 tig0041342:+15716 Msa1461200:three_prime_UTR
ACTTCAGTTGTCAGTCCAGT+AGG 0.456008 tig0041342:-14956 None:intergenic
CTTCAGGATGTCTACAAGAT+TGG 0.458119 tig0041342:+14860 Msa1461200:CDS
GATTCCAACCATGAAATTGA+TGG 0.461383 tig0041342:+15752 Msa1461200:three_prime_UTR
AGTCTCAACCACCATGGGCT+TGG 0.462350 tig0041342:-15332 None:intergenic
ACCAAATCAATGAGCCTAAG+AGG 0.463105 tig0041342:+14810 Msa1461200:CDS
TGGGTCATCCTTGGAGTTTG+AGG 0.465051 tig0041342:-15089 None:intergenic
CACAAACCTTGACTGGTACA+AGG 0.466653 tig0041342:+14763 Msa1461200:CDS
CTCAACCACCATGGGCTTGG+TGG 0.468734 tig0041342:-15329 None:intergenic
TAGGCTCATTGATTTGGTCA+AGG 0.472707 tig0041342:-14805 None:intergenic
GGCTTGTCTGAAGGCCTCTT+AGG 0.473139 tig0041342:-14824 None:intergenic
TAGCCATTTCCAATCTGGCC+AGG 0.474048 tig0041342:-15160 None:intergenic
AGGCTCATTGATTTGGTCAA+GGG 0.475242 tig0041342:-14804 None:intergenic
TGCACTGTCATTGATGCCCC+TGG 0.477178 tig0041342:+14290 Msa1461200:CDS
TTAATGAAATCCCTGTGTCC+AGG 0.479234 tig0041342:-14308 None:intergenic
AGTAAAGCTAGCAGCCTCCT+TGG 0.479718 tig0041342:-15113 None:intergenic
AGTCAGCTTTGTTAGATGGC+AGG 0.481378 tig0041342:-15634 None:intergenic
TCACCATTGACATTGCTCTC+TGG 0.481623 tig0041342:+14243 Msa1461200:CDS
AGCAGGATGATCTATCTAGT+TGG 0.481793 tig0041342:+15500 Msa1461200:three_prime_UTR
AGCTTGTTACTAAGATTGAT+AGG 0.481965 tig0041342:+15233 Msa1461200:CDS
ACAGTCAGCTTGGGATGTTC+CGG 0.483896 tig0041342:-14342 None:intergenic
AGCTAGCAGCCTCCTTGGCT+GGG 0.486002 tig0041342:-15108 None:intergenic
GCAGGATGATCTATCTAGTT+GGG 0.489479 tig0041342:+15501 Msa1461200:three_prime_UTR
TCATCCTATTTGAAGAAGGT+TGG 0.491235 tig0041342:+14671 Msa1461200:CDS
CTTGTACCAGTCAAGGTTTG+TGG 0.492698 tig0041342:-14762 None:intergenic
GATTGATAGGCGTTCTGGTA+AGG 0.495592 tig0041342:+15246 Msa1461200:CDS
ATCTCCCTCAAAACCAGAGA+TGG 0.496573 tig0041342:-14723 None:intergenic
TGAGAACGGCACAGTCAGCT+TGG 0.498317 tig0041342:-14352 None:intergenic
CTGACCATCCTTAGAAATAC+CGG 0.501461 tig0041342:-14405 None:intergenic
AGTTTCATCCTATTTGAAGA+AGG 0.501588 tig0041342:+14667 Msa1461200:CDS
ATCATTGATTCCACTACTGG+TGG 0.502099 tig0041342:+14374 Msa1461200:CDS
GAGAACGGCACAGTCAGCTT+GGG 0.505334 tig0041342:-14351 None:intergenic
GTCAGCTTTGTTAGATGGCA+GGG 0.505354 tig0041342:-15633 None:intergenic
CTTCAGTTGTCAGTCCAGTA+GGG 0.506099 tig0041342:-14955 None:intergenic
AATTTCATCATATCTACCCT+TGG 0.508007 tig0041342:-14636 None:intergenic
AATGACAGTGCAGTAGTACT+TGG 0.509426 tig0041342:-14279 None:intergenic
CATCAAGGCTGTGGAGAAGA+AGG 0.510945 tig0041342:+15423 Msa1461200:CDS
AGGAATTGGAACTGTGCCTG+TGG 0.517140 tig0041342:+14883 Msa1461200:CDS
GGTCACCTTAAGTCCTCCAC+TGG 0.520286 tig0041342:-15449 None:intergenic
GAAGAAGAAGTGAACCGAGC+AGG 0.523086 tig0041342:+15483 Msa1461200:exon
CAGGATGTCTACAAGATTGG+AGG 0.523217 tig0041342:+14863 Msa1461200:CDS
TCTTCTAAAGAGAGATAAGC+GGG 0.525356 tig0041342:-15938 None:intergenic
TAATGAAATCCCTGTGTCCA+GGG 0.528272 tig0041342:-14307 None:intergenic
GAGCCCAAATTCTTGAAGAA+TGG 0.528480 tig0041342:+15280 Msa1461200:CDS
TCCCACATTGTCACCAGGAA+GGG 0.528721 tig0041342:-15017 None:intergenic
CGGTCCATCAATTTCATGGT+TGG 0.529458 tig0041342:-15756 None:intergenic
CATTGATGCCCCTGGACACA+GGG 0.535845 tig0041342:+14298 Msa1461200:CDS
GGAATTGGAACTGTGCCTGT+GGG 0.536194 tig0041342:+14884 Msa1461200:CDS
TCATTGATGCCCCTGGACAC+AGG 0.539611 tig0041342:+14297 Msa1461200:CDS
ATAAATAGGAAATACGATCC+TGG 0.539894 tig0041342:+15829 Msa1461200:three_prime_UTR
CTTCCAGAGAGCAATGTCAA+TGG 0.541189 tig0041342:-14246 None:intergenic
CATATCAACATTGTGGTCAT+CGG 0.541947 tig0041342:+14050 Msa1461200:CDS
AGTCGAAGGGGCTTGTCTGA+AGG 0.542120 tig0041342:-14833 None:intergenic
CACTTGATCTACAAGCTTGG+AGG 0.545798 tig0041342:+14107 Msa1461200:CDS
GGCTTCAAAACCACCAGTAG+TGG 0.551737 tig0041342:-14384 None:intergenic
TGTGGCTGTTGGCGTCATCA+AGG 0.552638 tig0041342:+15408 Msa1461200:CDS
AAGAGAGAATTACCTTGAAG+AGG 0.552753 tig0041342:-12758 None:intergenic
GGTCACTTGATCTACAAGCT+TGG 0.555547 tig0041342:+14104 Msa1461200:CDS
CCAGTCTCGACACGCCCCAC+AGG 0.557031 tig0041342:-14899 None:intergenic
TCAAGGGCCTCAAGAAGGGT+TGG 0.557590 tig0041342:-14788 None:intergenic
TCAACCACCATGGGCTTGGT+GGG 0.557624 tig0041342:-15328 None:intergenic
TTGGTCAAGGGCCTCAAGAA+GGG 0.558003 tig0041342:-14792 None:intergenic
TTGGACCCTTGTACCAGTCA+AGG 0.558366 tig0041342:-14769 None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG 0.559179 tig0041342:-14430 None:intergenic
AGCGCTCCACAAACCTTGAC+TGG 0.559246 tig0041342:+14756 Msa1461200:CDS
CTCAAGGCTGAACGTGAAAG+AGG 0.559327 tig0041342:+14218 Msa1461200:CDS
TTTGGTCAAGGGCCTCAAGA+AGG 0.560467 tig0041342:-14793 None:intergenic
TTCTTGAAGAATGGTGATGC+TGG 0.561159 tig0041342:+15289 Msa1461200:CDS
GGAATCTCCACTCTATTGAC+CGG 0.562074 tig0041342:-15735 None:intergenic
TCTGGGAAATCAACCACCAC+TGG 0.562550 tig0041342:+14083 Msa1461200:CDS
GAAGCCGGTATTTCTAAGGA+TGG 0.564413 tig0041342:+14401 Msa1461200:CDS
GTAGCATATCCGCAAGACCT+TGG 0.566509 tig0041342:+15671 Msa1461200:three_prime_UTR
ACAAACCTTGACTGGTACAA+GGG 0.567625 tig0041342:+14764 Msa1461200:CDS
TCATCGGCCATGTTGACTCT+GGG 0.571507 tig0041342:+14066 Msa1461200:CDS
TTCATTAAGAACATGATCAC+CGG 0.572337 tig0041342:+14323 Msa1461200:CDS
AGCAAGGAGAGCATGTTCAC+GGG 0.572470 tig0041342:-14429 None:intergenic
GAGAAAGTCTCAACCACCAT+GGG 0.575954 tig0041342:-15337 None:intergenic
ATCCTGAAGTGGTAGTCGAA+GGG 0.576252 tig0041342:-14846 None:intergenic
CTTGAGCTTGTCAAGGACCC+AGG 0.577041 tig0041342:-14201 None:intergenic
TGAATTGAACCAAGGTCTTG+CGG 0.577239 tig0041342:-15680 None:intergenic
TATCTACCCTTGGAGTACTT+GGG 0.580849 tig0041342:-14626 None:intergenic
GCATCAGTCTTGTAAACTGA+AGG 0.582139 tig0041342:+13760 Msa1461200:five_prime_UTR
TTGACAAGCGTGTCATTGAG+AGG 0.584549 tig0041342:+14132 Msa1461200:CDS
ATCCCACATTGTCACCAGGA+AGG 0.585536 tig0041342:-15018 None:intergenic
GAGACTGGTGTCATAAAACC+TGG 0.585699 tig0041342:+14914 Msa1461200:CDS
CTGGGTCCTTGACAAGCTCA+AGG 0.585742 tig0041342:+14202 Msa1461200:CDS
GGCCCTTCCTGGTGACAATG+TGG 0.586985 tig0041342:+15015 Msa1461200:CDS
GAGAATGTGAGAGTGGAGAG+TGG 0.588313 tig0041342:-12704 None:intergenic
CTGTTAAGGATCTCAAGCGT+GGG 0.589274 tig0041342:+15059 Msa1461200:CDS
CATCCTGAAGTGGTAGTCGA+AGG 0.591035 tig0041342:-14847 None:intergenic
TTCTCTGAATATCCTCCACT+CGG 0.591172 tig0041342:+15355 Msa1461200:CDS
GCCCTTCCTGGTGACAATGT+GGG 0.592402 tig0041342:+15016 Msa1461200:CDS
TTGATTTCCCAGAGTCAACA+TGG 0.596206 tig0041342:-14073 None:intergenic
TAGATATGATGAAATTGTGA+AGG 0.599770 tig0041342:+14643 Msa1461200:CDS
GCATGTCTCTAACAGCAAAA+CGG 0.600985 tig0041342:-15378 None:intergenic
CTCCAAGGATGACCCAGCCA+AGG 0.602045 tig0041342:+15096 Msa1461200:CDS
GCGTTCATTCAAGTATGCCT+GGG 0.605879 tig0041342:+14184 Msa1461200:CDS
GTCACCTTAAGTCCTCCACT+GGG 0.606003 tig0041342:-15448 None:intergenic
TCTCCCTCAAAACCAGAGAT+GGG 0.607478 tig0041342:-14722 None:intergenic
ATAGATTCCGGTCAATAGAG+TGG 0.610318 tig0041342:+15728 Msa1461200:three_prime_UTR
GATGATTCCCACCAAGCCCA+TGG 0.611182 tig0041342:+15321 Msa1461200:CDS
TACTACACCCAAGTACTCCA+AGG 0.613862 tig0041342:+14619 Msa1461200:CDS
CTCTCACATTCTCATCCTTG+CGG 0.614676 tig0041342:+12714 Msa1461200:five_prime_UTR
AAAGGTTCATATCAACATTG+TGG 0.619488 tig0041342:+14043 Msa1461200:CDS
CAAGGGTCCAACCCTTCTTG+AGG 0.620566 tig0041342:+14781 Msa1461200:CDS
TCACACCAAGAGTGAAAGCA+AGG 0.621193 tig0041342:-14445 None:intergenic
ATCTTGTAGACATCCTGAAG+TGG 0.621388 tig0041342:-14857 None:intergenic
GCTGTTAAGGATCTCAAGCG+TGG 0.622485 tig0041342:+15058 Msa1461200:CDS
TGTTGGCGTCATCAAGGCTG+TGG 0.622557 tig0041342:+15414 Msa1461200:CDS
TTCAGTTGTCAGTCCAGTAG+GGG 0.622733 tig0041342:-14954 None:intergenic
AGCCATTTCCAATCTGGCCA+GGG 0.623271 tig0041342:-15159 None:intergenic
GACAACTGAAGTCAAGTCTG+TGG 0.624055 tig0041342:+14967 Msa1461200:CDS
CAAGGATGAGAATGTGAGAG+TGG 0.624166 tig0041342:-12711 None:intergenic
ACAGCAAAACGGCCGAGTGG+AGG 0.624787 tig0041342:-15367 None:intergenic
TGGTGTCATAAAACCTGGAA+TGG 0.625384 tig0041342:+14919 Msa1461200:CDS
TAATAGTTTGAATTGAACCA+AGG 0.627102 tig0041342:-15688 None:intergenic
TCCTGAAGTGGTAGTCGAAG+GGG 0.627928 tig0041342:-14845 None:intergenic
AGGTGTGGCAGTCAAGAACA+GGG 0.628146 tig0041342:-15186 None:intergenic
GTTCAGCCTTGAGCTTGTCA+AGG 0.628494 tig0041342:-14208 None:intergenic
GACGAGAAATAGAAATACCA+AGG 0.629840 tig0041342:-15561 None:intergenic
GCATCATGAAGCTCTAACAG+AGG 0.633418 tig0041342:+14994 Msa1461200:CDS
GTATGTTGCCTCAAACTCCA+AGG 0.633897 tig0041342:+15081 Msa1461200:CDS
AGAGAAAGTCTCAACCACCA+TGG 0.641605 tig0041342:-15338 None:intergenic
ATGCGTCAAACTGTGGCTGT+TGG 0.643159 tig0041342:+15397 Msa1461200:CDS
ACTAGATAGATCATCCTGCT+CGG 0.643222 tig0041342:-15497 None:intergenic
TTCTGGTAAGGAGATTGAGA+AGG 0.643722 tig0041342:+15258 Msa1461200:CDS
GAATTGGAACTGTGCCTGTG+GGG 0.644316 tig0041342:+14885 Msa1461200:CDS
AGTGGAATCAATGATGAGAA+CGG 0.644693 tig0041342:-14366 None:intergenic
TAAGAGACAAATAAATGTAG+CGG 0.648524 tig0041342:-15776 None:intergenic
TTCTTCTAAAGAGAGATAAG+CGG 0.652423 tig0041342:-15939 None:intergenic
AAACTTGACAGCAATGTGAG+AGG 0.653594 tig0041342:-15206 None:intergenic
AATGAAATCCCTGTGTCCAG+GGG 0.656838 tig0041342:-14306 None:intergenic
TTAAATCCCACATTGTCACC+AGG 0.657091 tig0041342:-15022 None:intergenic
AATGATCTGCTGTTGCAACA+AGG 0.659392 tig0041342:+14472 Msa1461200:CDS
CAAGGATGACCCAGCCAAGG+AGG 0.659665 tig0041342:+15099 Msa1461200:CDS
GAGAAGAAGGATCCCAGTGG+AGG 0.667988 tig0041342:+15436 Msa1461200:CDS
ACTACACCCAAGTACTCCAA+GGG 0.678033 tig0041342:+14620 Msa1461200:CDS
CTTGTAGATCAAGTGACCAG+TGG 0.682705 tig0041342:-14099 None:intergenic
TAGAGACATGCGTCAAACTG+TGG 0.690315 tig0041342:+15390 Msa1461200:CDS
CTAACAGCAAAACGGCCGAG+TGG 0.692072 tig0041342:-15370 None:intergenic
GTAGATCAAGTGACCAGTGG+TGG 0.699002 tig0041342:-14096 None:intergenic
GTGATCATAATGAATCACCC+TGG 0.699056 tig0041342:+15142 Msa1461200:CDS
AACTTGCCCTTATAGCTCAG+TGG 0.706507 tig0041342:+13733 Msa1461200:five_prime_UTR
GATTCCCACCAAGCCCATGG+TGG 0.723849 tig0041342:+15324 Msa1461200:CDS
GGTGTGGCAGTCAAGAACAG+GGG 0.727394 tig0041342:-15185 None:intergenic
TGACAGCAATGTGAGAGGTG+TGG 0.729691 tig0041342:-15201 None:intergenic
GTGGAGAAGAAGGATCCCAG+TGG 0.734762 tig0041342:+15433 Msa1461200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAATCTTTTGTTAATTTT+TGG + tig0041342:13015-13034 Msa1461200:intron 10.0%
!!! TTAATCTTTTGTTAATTTTT+GGG + tig0041342:13016-13035 Msa1461200:intron 10.0%
!!! TTTTTGTTTTCTTTTTGATT+TGG + tig0041342:12893-12912 Msa1461200:intron 15.0%
!! GATAATTATTGTTATATTGT+TGG + tig0041342:13630-13649 Msa1461200:intron 15.0%
!! ATCATAGTTATTACAATAAA+TGG + tig0041342:15530-15549 Msa1461200:three_prime_UTR 15.0%
!! CAAACTATTACAAATAAAAT+AGG + tig0041342:15701-15720 Msa1461200:three_prime_UTR 15.0%
!! ATTCATAACAACAATTAATC+AGG - tig0041342:12954-12973 None:intergenic 20.0%
!! TGTTGTTATGAATTCATATA+CGG + tig0041342:12961-12980 Msa1461200:intron 20.0%
!!! AATCGTTTATGTTTTTGAAT+CGG + tig0041342:12988-13007 Msa1461200:intron 20.0%
!!! TCAATAATGGAATATGTATT+GGG - tig0041342:13395-13414 None:intergenic 20.0%
!!! TTGTTTTTTTTCAGCTTTTA+AGG + tig0041342:14005-14024 Msa1461200:five_prime_UTR 20.0%
!! AATCAAATATACAACTTCAA+GGG - tig0041342:14515-14534 None:intergenic 20.0%
!! TAATCAAATATACAACTTCA+AGG - tig0041342:14516-14535 None:intergenic 20.0%
!! TTATTACAATAAATGGTCTT+TGG + tig0041342:15537-15556 Msa1461200:three_prime_UTR 20.0%
!! TAATTCTATCAAATTGTTTC+TGG + tig0041342:15906-15925 Msa1461200:three_prime_UTR 20.0%
!!! TGTGTAAATTTGTTTGCTTT+TGG + tig0041342:13342-13361 Msa1461200:intron 25.0%
!!! GTGTAAATTTGTTTGCTTTT+GGG + tig0041342:13343-13362 Msa1461200:intron 25.0%
!! GTCAATAATGGAATATGTAT+TGG - tig0041342:13396-13415 None:intergenic 25.0%
! TACATATTCCATTATTGACA+TGG + tig0041342:13397-13416 Msa1461200:intron 25.0%
! AACTCTGACAAAAAAAAATG+AGG - tig0041342:13706-13725 None:intergenic 25.0%
!! TTCCTTTACTATTTTCTTTG+AGG + tig0041342:13949-13968 Msa1461200:intron 25.0%
! ATCAAATATACAACTTCAAG+GGG - tig0041342:14514-14533 None:intergenic 25.0%
! TAGATATGATGAAATTGTGA+AGG + tig0041342:14643-14662 Msa1461200:CDS 25.0%
! AATTTCTTATTGCTTGAGTT+TGG - tig0041342:15611-15630 None:intergenic 25.0%
! TAATAGTTTGAATTGAACCA+AGG - tig0041342:15691-15710 None:intergenic 25.0%
! AAAATAGGTAACATAGATTC+CGG + tig0041342:15716-15735 Msa1461200:three_prime_UTR 25.0%
! TAAGAGACAAATAAATGTAG+CGG - tig0041342:15779-15798 None:intergenic 25.0%
! TGGACAATGAACAAATAAAT+AGG + tig0041342:15815-15834 Msa1461200:three_prime_UTR 25.0%
!!! AATTATGATTCGCTATTTTG+TGG - tig0041342:15891-15910 None:intergenic 25.0%
GTCAACAACACAATATAGAT+TGG - tig0041342:13150-13169 None:intergenic 30.0%
AACACACACATACACTTATT+TGG + tig0041342:13189-13208 Msa1461200:intron 30.0%
ACACACACATACACTTATTT+GGG + tig0041342:13190-13209 Msa1461200:intron 30.0%
CATGCTTCAAAAGAAAAAAG+GGG - tig0041342:13251-13270 None:intergenic 30.0%
GCATGCTTCAAAAGAAAAAA+GGG - tig0041342:13252-13271 None:intergenic 30.0%
AGCATGCTTCAAAAGAAAAA+AGG - tig0041342:13253-13272 None:intergenic 30.0%
! TGCTTGTTGAATTATTGACT+TGG + tig0041342:13294-13313 Msa1461200:intron 30.0%
TTCTATCACCATGTCAATAA+TGG - tig0041342:13408-13427 None:intergenic 30.0%
TATAAATCAGACATGCAATC+AGG - tig0041342:13503-13522 None:intergenic 30.0%
GACCTCAAAGAAAATAGTAA+AGG - tig0041342:13954-13973 None:intergenic 30.0%
! TGCTTATGCATAGTTTTCTT+TGG + tig0041342:13975-13994 Msa1461200:intron 30.0%
!! TTTCAGCTTTTAAGGACTAA+TGG + tig0041342:14013-14032 Msa1461200:exon 30.0%
!! TTCAGCTTTTAAGGACTAAT+GGG + tig0041342:14014-14033 Msa1461200:exon 30.0%
AAAGGTTCATATCAACATTG+TGG + tig0041342:14043-14062 Msa1461200:CDS 30.0%
TTCATTAAGAACATGATCAC+CGG + tig0041342:14323-14342 Msa1461200:CDS 30.0%
AATTTCATCATATCTACCCT+TGG - tig0041342:14639-14658 None:intergenic 30.0%
AGTTTCATCCTATTTGAAGA+AGG + tig0041342:14667-14686 Msa1461200:CDS 30.0%
! GAACAAATGGAATTTTGTCT+GGG - tig0041342:14703-14722 None:intergenic 30.0%
!! AGCTTGTTACTAAGATTGAT+AGG + tig0041342:15233-15252 Msa1461200:CDS 30.0%
TCACCATTCTTCAAGAATTT+GGG - tig0041342:15286-15305 None:intergenic 30.0%
ATCACCATTCTTCAAGAATT+TGG - tig0041342:15287-15306 None:intergenic 30.0%
!! AAAAAGTCAGCTTTGTTAGA+TGG - tig0041342:15641-15660 None:intergenic 30.0%
!! ATCTAACAAAGCTGACTTTT+TGG + tig0041342:15640-15659 Msa1461200:three_prime_UTR 30.0%
CTTAGTAAACTCAATGAGTT+TGG + tig0041342:15795-15814 Msa1461200:three_prime_UTR 30.0%
ATAAATAGGAAATACGATCC+TGG + tig0041342:15829-15848 Msa1461200:three_prime_UTR 30.0%
!!! TAGTTTTGATCTCATTATCC+AGG - tig0041342:15850-15869 None:intergenic 30.0%
! TTCTTCTAAAGAGAGATAAG+CGG - tig0041342:15942-15961 None:intergenic 30.0%
AAGAGAGAATTACCTTGAAG+AGG - tig0041342:12761-12780 None:intergenic 35.0%
TGCATGGGATCATTACAAAT+TGG + tig0041342:12866-12885 Msa1461200:intron 35.0%
TTACACACACATACTTGTTC+AGG + tig0041342:13545-13564 Msa1461200:intron 35.0%
ATTATCATCAGCATGCTTCA+AGG - tig0041342:13616-13635 None:intergenic 35.0%
!! CTTTTAAGGACTAATGGGTA+AGG + tig0041342:14019-14038 Msa1461200:exon 35.0%
CATATCAACATTGTGGTCAT+CGG + tig0041342:14050-14069 Msa1461200:CDS 35.0%
AGTGGAATCAATGATGAGAA+CGG - tig0041342:14369-14388 None:intergenic 35.0%
!!! TTTTGAAGCCGGTATTTCTA+AGG + tig0041342:14397-14416 Msa1461200:CDS 35.0%
ATCCATCTGTTCAAGATAGA+TGG - tig0041342:14597-14616 None:intergenic 35.0%
TCATCCTATTTGAAGAAGGT+TGG + tig0041342:14671-14690 Msa1461200:CDS 35.0%
ATAGCCAACCTTCTTCAAAT+AGG - tig0041342:14678-14697 None:intergenic 35.0%
! GGAACAAATGGAATTTTGTC+TGG - tig0041342:14704-14723 None:intergenic 35.0%
! TGTCAAGAATGTTGCTGTTA+AGG + tig0041342:15045-15064 Msa1461200:CDS 35.0%
AATAAATGGTCTTTGGTCCT+TGG + tig0041342:15544-15563 Msa1461200:three_prime_UTR 35.0%
GACGAGAAATAGAAATACCA+AGG - tig0041342:15564-15583 None:intergenic 35.0%
GATTCCAACCATGAAATTGA+TGG + tig0041342:15752-15771 Msa1461200:three_prime_UTR 35.0%
! TCTTCTAAAGAGAGATAAGC+GGG - tig0041342:15941-15960 None:intergenic 35.0%
AGCTTATGTACGTGATTGCA+TGG + tig0041342:12850-12869 Msa1461200:intron 40.0%
GCTTATGTACGTGATTGCAT+GGG + tig0041342:12851-12870 Msa1461200:intron 40.0%
AATAGAGTAGAGTAACGCTG+TGG + tig0041342:13223-13242 Msa1461200:intron 40.0%
! TTGTTCAGGAACTGACTCAA+TGG + tig0041342:13559-13578 Msa1461200:intron 40.0%
! TCAATGGAGTCCTTTTAACC+CGG + tig0041342:13575-13594 Msa1461200:intron 40.0%
TCAGCATGCTTCAAGGAAAA+CGG - tig0041342:13609-13628 None:intergenic 40.0%
! GCATCAGTCTTGTAAACTGA+AGG + tig0041342:13760-13779 Msa1461200:five_prime_UTR 40.0%
! CAAGTCTTATCCCACTAAGT+GGG - tig0041342:13836-13855 None:intergenic 40.0%
! AGGACTAATGGGTAAGGAAA+AGG + tig0041342:14025-14044 Msa1461200:exon 40.0%
TTGATTTCCCAGAGTCAACA+TGG - tig0041342:14076-14095 None:intergenic 40.0%
AATGACAGTGCAGTAGTACT+TGG - tig0041342:14282-14301 None:intergenic 40.0%
TAATGAAATCCCTGTGTCCA+GGG - tig0041342:14310-14329 None:intergenic 40.0%
TTAATGAAATCCCTGTGTCC+AGG - tig0041342:14311-14330 None:intergenic 40.0%
!! CTCATCATTGATTCCACTAC+TGG + tig0041342:14371-14390 Msa1461200:CDS 40.0%
!! ATCATTGATTCCACTACTGG+TGG + tig0041342:14374-14393 Msa1461200:CDS 40.0%
CTGACCATCCTTAGAAATAC+CGG - tig0041342:14408-14427 None:intergenic 40.0%
AATGATCTGCTGTTGCAACA+AGG + tig0041342:14472-14491 Msa1461200:CDS 40.0%
TGCCATCTATCTTGAACAGA+TGG + tig0041342:14592-14611 Msa1461200:intron 40.0%
! TATCTACCCTTGGAGTACTT+GGG - tig0041342:14629-14648 None:intergenic 40.0%
! ATATCTACCCTTGGAGTACT+TGG - tig0041342:14630-14649 None:intergenic 40.0%
ATTCCATTTGTTCCCATCTC+TGG + tig0041342:14710-14729 Msa1461200:CDS 40.0%
AAACCAGAGATGGGAACAAA+TGG - tig0041342:14716-14735 None:intergenic 40.0%
ACAAACCTTGACTGGTACAA+GGG + tig0041342:14764-14783 Msa1461200:CDS 40.0%
!! AGGCTCATTGATTTGGTCAA+GGG - tig0041342:14807-14826 None:intergenic 40.0%
!! TAGGCTCATTGATTTGGTCA+AGG - tig0041342:14808-14827 None:intergenic 40.0%
ACCAAATCAATGAGCCTAAG+AGG + tig0041342:14810-14829 Msa1461200:CDS 40.0%
ATCTTGTAGACATCCTGAAG+TGG - tig0041342:14860-14879 None:intergenic 40.0%
CTTCAGGATGTCTACAAGAT+TGG + tig0041342:14860-14879 Msa1461200:CDS 40.0%
GTCTACAAGATTGGAGGAAT+TGG + tig0041342:14869-14888 Msa1461200:CDS 40.0%
!! TGGTGTCATAAAACCTGGAA+TGG + tig0041342:14919-14938 Msa1461200:CDS 40.0%
TTAAATCCCACATTGTCACC+AGG - tig0041342:15025-15044 None:intergenic 40.0%
GTGATCATAATGAATCACCC+TGG + tig0041342:15142-15161 Msa1461200:CDS 40.0%
AAACTTGACAGCAATGTGAG+AGG - tig0041342:15209-15228 None:intergenic 40.0%
ACTAAGATTGATAGGCGTTC+TGG + tig0041342:15241-15260 Msa1461200:CDS 40.0%
TTCTGGTAAGGAGATTGAGA+AGG + tig0041342:15258-15277 Msa1461200:CDS 40.0%
GAGCCCAAATTCTTGAAGAA+TGG + tig0041342:15280-15299 Msa1461200:CDS 40.0%
! TTCTTGAAGAATGGTGATGC+TGG + tig0041342:15289-15308 Msa1461200:CDS 40.0%
TTCTCTGAATATCCTCCACT+CGG + tig0041342:15355-15374 Msa1461200:CDS 40.0%
GCATGTCTCTAACAGCAAAA+CGG - tig0041342:15381-15400 None:intergenic 40.0%
! CTTCTTCTTCAATGCTGACT+TGG - tig0041342:15473-15492 None:intergenic 40.0%
ACTAGATAGATCATCCTGCT+CGG - tig0041342:15500-15519 None:intergenic 40.0%
AGCAGGATGATCTATCTAGT+TGG + tig0041342:15500-15519 Msa1461200:three_prime_UTR 40.0%
GCAGGATGATCTATCTAGTT+GGG + tig0041342:15501-15520 Msa1461200:three_prime_UTR 40.0%
!! CAAAGCTGACTTTTTGGCAT+AGG + tig0041342:15646-15665 Msa1461200:three_prime_UTR 40.0%
TGAATTGAACCAAGGTCTTG+CGG - tig0041342:15683-15702 None:intergenic 40.0%
ATAGATTCCGGTCAATAGAG+TGG + tig0041342:15728-15747 Msa1461200:three_prime_UTR 40.0%
!!! CGCTATTTTGTGGATTAGAG+AGG - tig0041342:15881-15900 None:intergenic 40.0%
CAAGGATGAGAATGTGAGAG+TGG - tig0041342:12714-12733 None:intergenic 45.0%
CTCTCACATTCTCATCCTTG+CGG + tig0041342:12714-12733 Msa1461200:five_prime_UTR 45.0%
AGCATGCTTCAAGGAAAACG+GGG - tig0041342:13607-13626 None:intergenic 45.0%
CAGCATGCTTCAAGGAAAAC+GGG - tig0041342:13608-13627 None:intergenic 45.0%
AACTTGCCCTTATAGCTCAG+TGG + tig0041342:13733-13752 Msa1461200:five_prime_UTR 45.0%
GCTCTACCACTGAGCTATAA+GGG - tig0041342:13742-13761 None:intergenic 45.0%
TGCTCTACCACTGAGCTATA+AGG - tig0041342:13743-13762 None:intergenic 45.0%
! CCAAGTCTTATCCCACTAAG+TGG - tig0041342:13837-13856 None:intergenic 45.0%
CCACTTAGTGGGATAAGACT+TGG + tig0041342:13834-13853 Msa1461200:intron 45.0%
CTTGTAGATCAAGTGACCAG+TGG - tig0041342:14102-14121 None:intergenic 45.0%
GGTCACTTGATCTACAAGCT+TGG + tig0041342:14104-14123 Msa1461200:CDS 45.0%
CACTTGATCTACAAGCTTGG+AGG + tig0041342:14107-14126 Msa1461200:CDS 45.0%
! TTGACAAGCGTGTCATTGAG+AGG + tig0041342:14132-14151 Msa1461200:CDS 45.0%
AGCGTTCATTCAAGTATGCC+TGG + tig0041342:14183-14202 Msa1461200:CDS 45.0%
GCGTTCATTCAAGTATGCCT+GGG + tig0041342:14184-14203 Msa1461200:CDS 45.0%
TCACCATTGACATTGCTCTC+TGG + tig0041342:14243-14262 Msa1461200:CDS 45.0%
CTTCCAGAGAGCAATGTCAA+TGG - tig0041342:14249-14268 None:intergenic 45.0%
AATGAAATCCCTGTGTCCAG+GGG - tig0041342:14309-14328 None:intergenic 45.0%
!!! ACTACTGGTGGTTTTGAAGC+CGG + tig0041342:14386-14405 Msa1461200:CDS 45.0%
! GAAGCCGGTATTTCTAAGGA+TGG + tig0041342:14401-14420 Msa1461200:CDS 45.0%
! AAGCAAGGAGAGCATGTTCA+CGG - tig0041342:14433-14452 None:intergenic 45.0%
TCACACCAAGAGTGAAAGCA+AGG - tig0041342:14448-14467 None:intergenic 45.0%
TACTACACCCAAGTACTCCA+AGG + tig0041342:14619-14638 Msa1461200:CDS 45.0%
ACTACACCCAAGTACTCCAA+GGG + tig0041342:14620-14639 Msa1461200:CDS 45.0%
!!! TGTTCCCATCTCTGGTTTTG+AGG + tig0041342:14718-14737 Msa1461200:CDS 45.0%
!!! GTTCCCATCTCTGGTTTTGA+GGG + tig0041342:14719-14738 Msa1461200:CDS 45.0%
TCTCCCTCAAAACCAGAGAT+GGG - tig0041342:14725-14744 None:intergenic 45.0%
ATCTCCCTCAAAACCAGAGA+TGG - tig0041342:14726-14745 None:intergenic 45.0%
CTTGTACCAGTCAAGGTTTG+TGG - tig0041342:14765-14784 None:intergenic 45.0%
CACAAACCTTGACTGGTACA+AGG + tig0041342:14763-14782 Msa1461200:CDS 45.0%
! GCCTCTTAGGCTCATTGATT+TGG - tig0041342:14814-14833 None:intergenic 45.0%
ATCCTGAAGTGGTAGTCGAA+GGG - tig0041342:14849-14868 None:intergenic 45.0%
CAGGATGTCTACAAGATTGG+AGG + tig0041342:14863-14882 Msa1461200:CDS 45.0%
!! GAGACTGGTGTCATAAAACC+TGG + tig0041342:14914-14933 Msa1461200:CDS 45.0%
GCAAAAGTGACAACCATTCC+AGG - tig0041342:14935-14954 None:intergenic 45.0%
TTCAGTTGTCAGTCCAGTAG+GGG - tig0041342:14957-14976 None:intergenic 45.0%
CTTCAGTTGTCAGTCCAGTA+GGG - tig0041342:14958-14977 None:intergenic 45.0%
ACTTCAGTTGTCAGTCCAGT+AGG - tig0041342:14959-14978 None:intergenic 45.0%
GACAACTGAAGTCAAGTCTG+TGG + tig0041342:14967-14986 Msa1461200:CDS 45.0%
! GCATCATGAAGCTCTAACAG+AGG + tig0041342:14994-15013 Msa1461200:CDS 45.0%
CTGTTAAGGATCTCAAGCGT+GGG + tig0041342:15059-15078 Msa1461200:CDS 45.0%
GTATGTTGCCTCAAACTCCA+AGG + tig0041342:15081-15100 Msa1461200:CDS 45.0%
GATTGATAGGCGTTCTGGTA+AGG + tig0041342:15246-15265 Msa1461200:CDS 45.0%
GAGAAAGTCTCAACCACCAT+GGG - tig0041342:15340-15359 None:intergenic 45.0%
AGAGAAAGTCTCAACCACCA+TGG - tig0041342:15341-15360 None:intergenic 45.0%
TAGAGACATGCGTCAAACTG+TGG + tig0041342:15390-15409 Msa1461200:CDS 45.0%
!! GTCAGCTTTGTTAGATGGCA+GGG - tig0041342:15636-15655 None:intergenic 45.0%
!! AGTCAGCTTTGTTAGATGGC+AGG - tig0041342:15637-15656 None:intergenic 45.0%
GGAATCTCCACTCTATTGAC+CGG - tig0041342:15738-15757 None:intergenic 45.0%
CGGTCCATCAATTTCATGGT+TGG - tig0041342:15759-15778 None:intergenic 45.0%
GTAGCGGTCCATCAATTTCA+TGG - tig0041342:15763-15782 None:intergenic 45.0%
GAGAATGTGAGAGTGGAGAG+TGG - tig0041342:12707-12726 None:intergenic 50.0%
ACATTCTCATCCTTGCGGCT+AGG + tig0041342:12719-12738 Msa1461200:five_prime_UTR 50.0%
CATTCTCATCCTTGCGGCTA+GGG + tig0041342:12720-12739 Msa1461200:five_prime_UTR 50.0%
TTGCGCAGCTTTCCTCTTCA+AGG + tig0041342:12746-12765 Msa1461200:five_prime_UTR 50.0%
! TGGAGTCCTTTTAACCCGGT+TGG + tig0041342:13579-13598 Msa1461200:intron 50.0%
! GGAGTCCTTTTAACCCGGTT+GGG + tig0041342:13580-13599 Msa1461200:intron 50.0%
GCATGCTTCAAGGAAAACGG+GGG - tig0041342:13606-13625 None:intergenic 50.0%
TTGTTGCCAAGCCCACTTAG+TGG + tig0041342:13822-13841 Msa1461200:intron 50.0%
TGTTGCCAAGCCCACTTAGT+GGG + tig0041342:13823-13842 Msa1461200:intron 50.0%
! CTTATCCCACTAAGTGGGCT+TGG - tig0041342:13831-13850 None:intergenic 50.0%
!! TCATCGGCCATGTTGACTCT+GGG + tig0041342:14066-14085 Msa1461200:CDS 50.0%
TCTGGGAAATCAACCACCAC+TGG + tig0041342:14083-14102 Msa1461200:CDS 50.0%
GTAGATCAAGTGACCAGTGG+TGG - tig0041342:14099-14118 None:intergenic 50.0%
GTTCAGCCTTGAGCTTGTCA+AGG - tig0041342:14211-14230 None:intergenic 50.0%
CTCAAGGCTGAACGTGAAAG+AGG + tig0041342:14218-14237 Msa1461200:CDS 50.0%
ACAGTCAGCTTGGGATGTTC+CGG - tig0041342:14345-14364 None:intergenic 50.0%
GGCTTCAAAACCACCAGTAG+TGG - tig0041342:14387-14406 None:intergenic 50.0%
! AGCAAGGAGAGCATGTTCAC+GGG - tig0041342:14432-14451 None:intergenic 50.0%
GCTCTCCTTGCTTTCACTCT+TGG + tig0041342:14440-14459 Msa1461200:CDS 50.0%
TTGGACCCTTGTACCAGTCA+AGG - tig0041342:14772-14791 None:intergenic 50.0%
TTGGTCAAGGGCCTCAAGAA+GGG - tig0041342:14795-14814 None:intergenic 50.0%
TTTGGTCAAGGGCCTCAAGA+AGG - tig0041342:14796-14815 None:intergenic 50.0%
TCCTGAAGTGGTAGTCGAAG+GGG - tig0041342:14848-14867 None:intergenic 50.0%
CATCCTGAAGTGGTAGTCGA+AGG - tig0041342:14850-14869 None:intergenic 50.0%
AGGAATTGGAACTGTGCCTG+TGG + tig0041342:14883-14902 Msa1461200:CDS 50.0%
GGAATTGGAACTGTGCCTGT+GGG + tig0041342:14884-14903 Msa1461200:CDS 50.0%
GAATTGGAACTGTGCCTGTG+GGG + tig0041342:14885-14904 Msa1461200:CDS 50.0%
!!! GTTGTCACTTTTGCCCCTAC+TGG + tig0041342:14941-14960 Msa1461200:CDS 50.0%
TCCCACATTGTCACCAGGAA+GGG - tig0041342:15020-15039 None:intergenic 50.0%
ATCCCACATTGTCACCAGGA+AGG - tig0041342:15021-15040 None:intergenic 50.0%
GCTGTTAAGGATCTCAAGCG+TGG + tig0041342:15058-15077 Msa1461200:CDS 50.0%
TGGGTCATCCTTGGAGTTTG+AGG - tig0041342:15092-15111 None:intergenic 50.0%
AGTAAAGCTAGCAGCCTCCT+TGG - tig0041342:15116-15135 None:intergenic 50.0%
ATGAATCACCCTGGCCAGAT+TGG + tig0041342:15151-15170 Msa1461200:CDS 50.0%
AGCCATTTCCAATCTGGCCA+GGG - tig0041342:15162-15181 None:intergenic 50.0%
TAGCCATTTCCAATCTGGCC+AGG - tig0041342:15163-15182 None:intergenic 50.0%
GGGCATAGCCATTTCCAATC+TGG - tig0041342:15168-15187 None:intergenic 50.0%
AGGTGTGGCAGTCAAGAACA+GGG - tig0041342:15189-15208 None:intergenic 50.0%
TGACAGCAATGTGAGAGGTG+TGG - tig0041342:15204-15223 None:intergenic 50.0%
ATGCGTCAAACTGTGGCTGT+TGG + tig0041342:15397-15416 Msa1461200:CDS 50.0%
CATCAAGGCTGTGGAGAAGA+AGG + tig0041342:15423-15442 Msa1461200:CDS 50.0%
GTCACCTTAAGTCCTCCACT+GGG - tig0041342:15451-15470 None:intergenic 50.0%
GAAGAAGAAGTGAACCGAGC+AGG + tig0041342:15483-15502 Msa1461200:exon 50.0%
GTAGCATATCCGCAAGACCT+TGG + tig0041342:15671-15690 Msa1461200:three_prime_UTR 50.0%
CCTCACGCAGGATCGAACTA+CGG - tig0041342:13787-13806 None:intergenic 55.0%
CGTAGTTCGATCCTGCGTGA+GGG + tig0041342:13785-13804 Msa1461200:intron 55.0%
GAATTGAACTGCCCTCACGC+AGG - tig0041342:13799-13818 None:intergenic 55.0%
!! GTCATCGGCCATGTTGACTC+TGG + tig0041342:14065-14084 Msa1461200:CDS 55.0%
CTTGAGCTTGTCAAGGACCC+AGG - tig0041342:14204-14223 None:intergenic 55.0%
CTGGGTCCTTGACAAGCTCA+AGG + tig0041342:14202-14221 Msa1461200:CDS 55.0%
TGCACTGTCATTGATGCCCC+TGG + tig0041342:14290-14309 Msa1461200:CDS 55.0%
TCATTGATGCCCCTGGACAC+AGG + tig0041342:14297-14316 Msa1461200:CDS 55.0%
CATTGATGCCCCTGGACACA+GGG + tig0041342:14298-14317 Msa1461200:CDS 55.0%
GAGAACGGCACAGTCAGCTT+GGG - tig0041342:14354-14373 None:intergenic 55.0%
TGAGAACGGCACAGTCAGCT+TGG - tig0041342:14355-14374 None:intergenic 55.0%
AGCGCTCCACAAACCTTGAC+TGG + tig0041342:14756-14775 Msa1461200:CDS 55.0%
CAAGGGTCCAACCCTTCTTG+AGG + tig0041342:14781-14800 Msa1461200:CDS 55.0%
TCAAGGGCCTCAAGAAGGGT+TGG - tig0041342:14791-14810 None:intergenic 55.0%
GGCTTGTCTGAAGGCCTCTT+AGG - tig0041342:14827-14846 None:intergenic 55.0%
AGTCGAAGGGGCTTGTCTGA+AGG - tig0041342:14836-14855 None:intergenic 55.0%
GCCCTTCCTGGTGACAATGT+GGG + tig0041342:15016-15035 Msa1461200:CDS 55.0%
CACCCTGGCCAGATTGGAAA+TGG + tig0041342:15157-15176 Msa1461200:CDS 55.0%
GGTGTGGCAGTCAAGAACAG+GGG - tig0041342:15188-15207 None:intergenic 55.0%
GAGGTGTGGCAGTCAAGAAC+AGG - tig0041342:15190-15209 None:intergenic 55.0%
GATGATTCCCACCAAGCCCA+TGG + tig0041342:15321-15340 Msa1461200:CDS 55.0%
! TCAACCACCATGGGCTTGGT+GGG - tig0041342:15331-15350 None:intergenic 55.0%
AGTCTCAACCACCATGGGCT+TGG - tig0041342:15335-15354 None:intergenic 55.0%
CTAACAGCAAAACGGCCGAG+TGG - tig0041342:15373-15392 None:intergenic 55.0%
TGTGGCTGTTGGCGTCATCA+AGG + tig0041342:15408-15427 Msa1461200:CDS 55.0%
TGTTGGCGTCATCAAGGCTG+TGG + tig0041342:15414-15433 Msa1461200:CDS 55.0%
GTGGAGAAGAAGGATCCCAG+TGG + tig0041342:15433-15452 Msa1461200:CDS 55.0%
GAGAAGAAGGATCCCAGTGG+AGG + tig0041342:15436-15455 Msa1461200:CDS 55.0%
GGATCCCAGTGGAGGACTTA+AGG + tig0041342:15444-15463 Msa1461200:CDS 55.0%
GGTCACCTTAAGTCCTCCAC+TGG - tig0041342:15452-15471 None:intergenic 55.0%
GCGCAAAAACCCTAGCCGCA+AGG - tig0041342:12732-12751 None:intergenic 60.0%
AAGGAAAACGGGGGCCCAAC+CGG - tig0041342:13597-13616 None:intergenic 60.0%
CCGTAGTTCGATCCTGCGTG+AGG + tig0041342:13784-13803 Msa1461200:intron 60.0%
GCCCCTTCGACTACCACTTC+AGG + tig0041342:14844-14863 Msa1461200:CDS 60.0%
GCTCTAACAGAGGCCCTTCC+TGG + tig0041342:15004-15023 Msa1461200:CDS 60.0%
GGCCCTTCCTGGTGACAATG+TGG + tig0041342:15015-15034 Msa1461200:CDS 60.0%
CTCCAAGGATGACCCAGCCA+AGG + tig0041342:15096-15115 Msa1461200:CDS 60.0%
CTCCTTGGCTGGGTCATCCT+TGG - tig0041342:15101-15120 None:intergenic 60.0%
CAAGGATGACCCAGCCAAGG+AGG + tig0041342:15099-15118 Msa1461200:CDS 60.0%
AGCTAGCAGCCTCCTTGGCT+GGG - tig0041342:15111-15130 None:intergenic 60.0%
AAGCTAGCAGCCTCCTTGGC+TGG - tig0041342:15112-15131 None:intergenic 60.0%
GATTCCCACCAAGCCCATGG+TGG + tig0041342:15324-15343 Msa1461200:CDS 60.0%
CTCAACCACCATGGGCTTGG+TGG - tig0041342:15332-15351 None:intergenic 60.0%
ACAGCAAAACGGCCGAGTGG+AGG - tig0041342:15370-15389 None:intergenic 60.0%
GGGGGCCCAACCGGGTTAAA+AGG - tig0041342:13588-13607 None:intergenic 65.0%
AGGAAAACGGGGGCCCAACC+GGG - tig0041342:13596-13615 None:intergenic 65.0%
CCAGTCTCGACACGCCCCAC+AGG - tig0041342:14902-14921 None:intergenic 70.0%
CCTGTGGGGCGTGTCGAGAC+TGG + tig0041342:14899-14918 Msa1461200:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0041342 gene 12698 15975 12698 ID=Msa1461200;Name=Msa1461200
tig0041342 mRNA 12698 15975 12698 ID=Msa1461200-mRNA-1;Parent=Msa1461200;Name=Msa1461200-mRNA-1;_AED=0.05;_eAED=0.05;_QI=161|1|1|1|0.33|0.25|4|480|448
tig0041342 exon 12698 12767 12698 ID=Msa1461200-mRNA-1:exon:23066;Parent=Msa1461200-mRNA-1
tig0041342 exon 13722 13781 13722 ID=Msa1461200-mRNA-1:exon:23067;Parent=Msa1461200-mRNA-1
tig0041342 exon 14001 14493 14001 ID=Msa1461200-mRNA-1:exon:23068;Parent=Msa1461200-mRNA-1
tig0041342 exon 14611 15975 14611 ID=Msa1461200-mRNA-1:exon:23069;Parent=Msa1461200-mRNA-1
tig0041342 five_prime_UTR 12698 12767 12698 ID=Msa1461200-mRNA-1:five_prime_utr;Parent=Msa1461200-mRNA-1
tig0041342 five_prime_UTR 13722 13781 13722 ID=Msa1461200-mRNA-1:five_prime_utr;Parent=Msa1461200-mRNA-1
tig0041342 five_prime_UTR 14001 14031 14001 ID=Msa1461200-mRNA-1:five_prime_utr;Parent=Msa1461200-mRNA-1
tig0041342 CDS 14032 14493 14032 ID=Msa1461200-mRNA-1:cds;Parent=Msa1461200-mRNA-1
tig0041342 CDS 14611 15495 14611 ID=Msa1461200-mRNA-1:cds;Parent=Msa1461200-mRNA-1
tig0041342 three_prime_UTR 15496 15975 15496 ID=Msa1461200-mRNA-1:three_prime_utr;Parent=Msa1461200-mRNA-1
Gene Sequence

>Msa1461200

ATGGGTAAGGAAAAGGTTCATATCAACATTGTGGTCATCGGCCATGTTGACTCTGGGAAATCAACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTCATTGAGAGGTTTGAGAAAGAAGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCCTGGGTCCTTGACAAGCTCAAGGCTGAACGTGAAAGAGGTATCACCATTGACATTGCTCTCTGGAAGTTTGAGACAACCAAGTACTACTGCACTGTCATTGATGCCCCTGGACACAGGGATTTCATTAAGAACATGATCACCGGAACATCCCAAGCTGACTGTGCCGTTCTCATCATTGATTCCACTACTGGTGGTTTTGAAGCCGGTATTTCTAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAAGCAAATGATCTGCTGTTGCAACAAGATGGATGCTACTACACCCAAGTACTCCAAGGGTAGATATGATGAAATTGTGAAGGAAGTTTCATCCTATTTGAAGAAGGTTGGCTATAACCCAGACAAAATTCCATTTGTTCCCATCTCTGGTTTTGAGGGAGATAACATGATTGAGCGCTCCACAAACCTTGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACCAAATCAATGAGCCTAAGAGGCCTTCAGACAAGCCCCTTCGACTACCACTTCAGGATGTCTACAAGATTGGAGGAATTGGAACTGTGCCTGTGGGGCGTGTCGAGACTGGTGTCATAAAACCTGGAATGGTTGTCACTTTTGCCCCTACTGGACTGACAACTGAAGTCAAGTCTGTGGAGATGCATCATGAAGCTCTAACAGAGGCCCTTCCTGGTGACAATGTGGGATTTAATGTCAAGAATGTTGCTGTTAAGGATCTCAAGCGTGGGTATGTTGCCTCAAACTCCAAGGATGACCCAGCCAAGGAGGCTGCTAGCTTTACTTCTCAAGTGATCATAATGAATCACCCTGGCCAGATTGGAAATGGCTATGCCCCTGTTCTTGACTGCCACACCTCTCACATTGCTGTCAAGTTTGCTGAGCTTGTTACTAAGATTGATAGGCGTTCTGGTAAGGAGATTGAGAAGGAGCCCAAATTCTTGAAGAATGGTGATGCTGGTATTATTAAGATGATTCCCACCAAGCCCATGGTGGTTGAGACTTTCTCTGAATATCCTCCACTCGGCCGTTTTGCTGTTAGAGACATGCGTCAAACTGTGGCTGTTGGCGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGTGGAGGACTTAAGGTGACCAAGTCAGCATTGAAGAAGAAGTGA
Protein sequence

>Msa1461200

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKGRYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPTGLTTEVKSVEMHHEALTEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKEIEKEPKFLKNGDAGIIKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKAVEKKDPSGGLKVTKSALKKK*