AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0177450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0177450 MtrunA17_Chr1g0200071 100.000 448 0 0 1 448 1 448 0.0 922
Msa0177450 MtrunA17_Chr1g0200091 100.000 448 0 0 1 448 1 448 0.0 922
Msa0177450 MtrunA17_Chr1g0200121 99.777 448 1 0 1 448 1 448 0.0 921
Msa0177450 MtrunA17_Chr6g0458111 96.652 448 14 1 1 448 1 447 0.0 895
Msa0177450 MtrunA17_Chr6g0458091 96.652 448 14 1 1 448 1 447 0.0 895
Msa0177450 MtrunA17_Chr4g0005781 96.429 448 15 1 1 448 1 447 0.0 890
Msa0177450 MtrunA17_Chr1g0150331 91.574 451 9 2 1 448 1 425 0.0 829
Msa0177450 MtrunA17_Chr1g0193591 82.719 434 75 0 1 434 1 434 0.0 744
Msa0177450 MtrunA17_Chr1g0204031 82.151 437 73 3 1 437 1 432 0.0 736
Msa0177450 MtrunA17_Chr1g0156421 79.263 434 90 0 1 434 1 434 0.0 728
Msa0177450 MtrunA17_Chr8g0340771 79.955 449 88 1 1 447 1 449 0.0 723
Msa0177450 MtrunA17_Chr5g0415391 73.048 397 107 0 39 435 1 397 0.0 597
Msa0177450 MtrunA17_Chr1g0196251 69.727 403 94 2 4 405 5 380 0.0 576
Msa0177450 MtrunA17_Chr8g0336511 78.593 327 69 1 1 326 66 392 0.0 543
Msa0177450 MtrunA17_Chr2g0319341 72.254 346 93 2 102 445 1 345 0.0 517
Msa0177450 MtrunA17_Chr2g0298371 68.923 325 82 2 102 424 1 308 3.20e-161 457
Msa0177450 MtrunA17_Chr8g0340371 70.124 241 69 2 1 240 1 239 1.91e-118 345
Msa0177450 MtrunA17_Chr1g0210931 63.087 298 56 4 155 447 1 249 5.74e-118 345
Msa0177450 MtrunA17_Chr2g0318291 71.681 226 59 3 1 223 1 224 2.50e-112 329
Msa0177450 MtrunA17_Chr6g0483121 37.963 432 254 6 5 428 89 514 3.35e-97 301
Msa0177450 MtrunA17_Chr8g0392641 37.963 432 258 6 4 429 143 570 2.46e-92 290
Msa0177450 MtrunA17_Chr8g0380091 36.028 433 268 6 7 433 275 704 8.55e-92 292
Msa0177450 MtrunA17_Chr1g0204041 86.452 155 21 0 1 155 1 155 1.05e-90 272
Msa0177450 MtrunA17_Chr1g0200111 76.271 177 32 1 264 430 1 177 1.04e-87 265
Msa0177450 MtrunA17_Chr2g0318281 68.421 190 60 0 243 432 5 194 3.08e-84 258
Msa0177450 MtrunA17_Chr1g0156411 81.429 140 26 0 6 145 10 149 9.94e-79 241
Msa0177450 MtrunA17_Chr8g0340361 60.541 185 57 2 264 433 1 184 2.27e-66 210
Msa0177450 MtrunA17_Chr4g0026961 54.639 194 72 4 195 385 32 212 9.25e-59 192
Msa0177450 MtrunA17_Chr4g0026961 76.190 42 10 0 1 42 1 42 6.34e-13 67.8
Msa0177450 MtrunA17_Chr2g0298381 77.778 99 22 0 1 99 1 99 6.32e-48 159
Msa0177450 MtrunA17_Chr8g0341021 76.471 102 24 0 3 104 4 105 1.17e-47 159
Msa0177450 MtrunA17_Chr7g0259481 30.365 438 245 14 3 429 59 447 1.16e-45 164
Msa0177450 MtrunA17_Chr3g0116821 29.955 444 250 15 3 429 88 487 6.59e-41 152
Msa0177450 MtrunA17_Chr1g0211481 79.747 79 16 0 5 83 4 82 3.54e-36 129
Msa0177450 MtrunA17_Chr2g0319351 82.192 73 13 0 5 77 5 77 4.27e-35 125
Msa0177450 MtrunA17_Chr1g0200101 89.231 65 7 0 172 236 49 113 7.74e-35 125
Msa0177450 MtrunA17_Chr1g0200101 73.469 49 13 0 21 69 2 50 8.71e-17 76.3
Msa0177450 MtrunA17_Chr2g0287231 32.389 247 135 6 1 244 10 227 8.03e-31 119
Msa0177450 MtrunA17_Chr8g0341041 35.000 220 80 5 213 432 2 158 7.54e-29 111
Msa0177450 MtrunA17_Chr7g0261801 69.697 66 17 1 155 220 1 63 3.98e-24 95.9
Msa0177450 MtrunA17_Chr3g0130991 26.471 272 143 9 9 272 85 307 1.10e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0177450 AT1G07920.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT1G07940.4 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT1G07940.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT1G07940.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT1G07940.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT5G60390.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT5G60390.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT5G60390.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT1G07930.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa0177450 AT1G07930.2 98.824 255 3 0 1 255 1 255 0.0 530
Msa0177450 AT1G07930.2 87.179 117 14 1 331 447 254 369 9.10e-65 213
Msa0177450 AT1G18070.1 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa0177450 AT1G18070.2 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa0177450 AT1G18070.3 36.927 436 256 6 5 424 99 531 3.87e-91 286
Msa0177450 AT5G10630.1 35.765 425 265 5 7 426 239 660 1.08e-90 289
Msa0177450 AT5G10630.5 35.765 425 265 5 7 426 240 661 1.11e-90 289
Msa0177450 AT5G10630.2 35.765 425 265 5 7 426 240 661 1.11e-90 289
Msa0177450 AT5G10630.4 35.765 425 265 5 7 426 315 736 6.71e-90 289
Msa0177450 AT5G10630.3 35.765 425 265 5 7 426 310 731 7.31e-90 288
Msa0177450 AT1G35550.1 78.641 103 22 0 335 437 1 103 1.47e-53 174
Msa0177450 AT4G02930.1 30.296 439 245 15 3 429 63 452 8.29e-44 159
Msa0177450 AT4G20360.1 30.137 438 257 10 3 429 75 474 9.60e-44 160
Msa0177450 AT4G20360.2 30.137 438 257 10 3 429 75 474 9.60e-44 160
Msa0177450 AT2G31060.3 25.000 264 160 8 9 263 61 295 3.24e-12 68.9
Msa0177450 AT5G08650.2 26.667 270 145 10 9 272 88 310 2.83e-11 66.2
Msa0177450 AT5G08650.1 26.667 270 145 10 9 272 88 310 2.86e-11 66.2
Msa0177450 AT2G31060.2 24.615 260 162 7 9 263 61 291 3.81e-11 65.9

Find 185 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACCATTCTTCAAGAATT+TGG 0.066665 1_4:+64405433 None:intergenic
TACTTGGCAATTGTAAAATT+TGG 0.117923 1_4:+64404801 None:intergenic
ATCATAGTTATTACAATAAA+TGG 0.259832 1_4:-64405187 Msa0177450:three_prime_UTR
TGCTGGGGCATGGTTCTATA+TGG 0.275303 1_4:-64404886 Msa0177450:three_prime_UTR
CTCCTTCTCAATTTCCTTAC+CGG 0.285465 1_4:+64405457 None:intergenic
GCTCTCCTTGCTTTCACTCT+TGG 0.289982 1_4:-64406257 Msa0177450:CDS
GTCTACAAGATTGGAGGAAT+TGG 0.315206 1_4:-64405848 Msa0177450:CDS
GCCTCTTAGGCTCATTGATT+TGG 0.315866 1_4:+64405906 None:intergenic
TTATTACAATAAATGGTCTT+TGG 0.319176 1_4:-64405180 Msa0177450:three_prime_UTR
TGTCAAGAATGTTGCTGTTA+AGG 0.325240 1_4:-64405672 Msa0177450:CDS
TCACCATTCTTCAAGAATTT+GGG 0.331757 1_4:+64405434 None:intergenic
ATAGCCAACCTTCTTCAAAT+AGG 0.332380 1_4:+64406042 None:intergenic
AAGTTAGCAGCCTCCTTGGC+TGG 0.333938 1_4:+64405608 None:intergenic
ACCTCGTGAAGAAGGGAAAA+AGG 0.344425 1_4:+64407936 None:intergenic
CTTCTTCTTCAATGCTGACT+TGG 0.345006 1_4:+64405247 None:intergenic
CACATCAACATTGTGGTCAT+TGG 0.354316 1_4:-64406647 Msa0177450:CDS
GAGGTGTGGCAGTCAAGAAC+AGG 0.357372 1_4:+64405530 None:intergenic
ATTCCATTTGTTCCCATCTC+TGG 0.368209 1_4:-64406007 Msa0177450:CDS
GCTCTCACAGAGGCCCTTCC+TGG 0.369900 1_4:-64405713 Msa0177450:CDS
CTCCTTGGCTGGGTCATCCT+TGG 0.371873 1_4:+64405619 None:intergenic
GTAAGCAAGCAGTAAGTTCC+AGG 0.376045 1_4:-64404981 Msa0177450:three_prime_UTR
ATATCTACCCTTGGAGTACT+TGG 0.380807 1_4:+64406090 None:intergenic
TCACCATTGACATTGCTCTT+TGG 0.386025 1_4:-64406454 Msa0177450:CDS
GTCATTGGCCATGTCGACTC+TGG 0.395247 1_4:-64406632 Msa0177450:CDS
TGCGTCAAACTGTAGCTGTT+GGG 0.401918 1_4:-64405319 Msa0177450:CDS
CCTGTGGGGCGTGTTGAAAC+TGG 0.405151 1_4:-64405818 Msa0177450:CDS
GCAAAAGTGACAACCATTCC+AGG 0.418006 1_4:+64405785 None:intergenic
CTCATCATTGATTCCACTAC+TGG 0.419293 1_4:-64406326 Msa0177450:CDS
TATGGTTTAGGCAATATATC+AGG 0.424066 1_4:-64404868 Msa0177450:three_prime_UTR
ATGAATCACCCTGGCCAGAT+TGG 0.425183 1_4:-64405566 Msa0177450:CDS
TCATTCCTTGCTACTCGACT+AGG 0.426624 1_4:-64407974 Msa0177450:five_prime_UTR
CTTAATGAAATCCCTGTGTC+CGG 0.429384 1_4:+64406388 None:intergenic
CACCCTGGCCAGATTGGAAA+TGG 0.432985 1_4:-64405560 Msa0177450:CDS
GCCCCTCCGACTACCACTTC+AGG 0.433104 1_4:-64405873 Msa0177450:CDS
ACTAAGATTGATAGGCGTTC+CGG 0.433848 1_4:-64405476 Msa0177450:CDS
TGACAAGCTTAAGGCTGAAC+GGG 0.436450 1_4:-64406486 Msa0177450:CDS
AGGACTAATGGGTAAGGAAA+AGG 0.440646 1_4:-64406672 Msa0177450:exon
AGCGTTCATTCAAGTATGCC+TGG 0.444655 1_4:-64406514 Msa0177450:CDS
GGCTTGTCTGAAGGCCTCTT+AGG 0.446650 1_4:+64405893 None:intergenic
GGGCATAGCCATTTCCAATC+TGG 0.448022 1_4:+64405552 None:intergenic
AAACCAGAGATGGGAACAAA+TGG 0.449170 1_4:+64406004 None:intergenic
GGTCTGCTTAAGTCCTCCAC+TGG 0.451209 1_4:+64405268 None:intergenic
GGCATGGTTCTATATGGTTT+AGG 0.454376 1_4:-64404880 Msa0177450:three_prime_UTR
ACTTCAGTTGTCAGTCCAGT+AGG 0.456008 1_4:+64405761 None:intergenic
CTTCAGGATGTCTACAAGAT+TGG 0.458119 1_4:-64405857 Msa0177450:CDS
CTCGACCACCATGGGCTTGG+TGG 0.461350 1_4:+64405388 None:intergenic
ACCAAATCAATGAGCCTAAG+AGG 0.463105 1_4:-64405907 Msa0177450:CDS
CTGGGTCCTTGACAAGCTTA+AGG 0.464518 1_4:-64406495 Msa0177450:CDS
TGGGTCATCCTTGGAGTTTG+AGG 0.465051 1_4:+64405628 None:intergenic
CACAAACCTTGACTGGTACA+AGG 0.466653 1_4:-64405954 Msa0177450:CDS
GGGTTGTTACCTCGTGAAGA+AGG 0.471526 1_4:+64407928 None:intergenic
TAGGCTCATTGATTTGGTCA+AGG 0.472707 1_4:+64405912 None:intergenic
TTAATGAAATCCCTGTGTCC+GGG 0.473933 1_4:+64406389 None:intergenic
TAGCCATTTCCAATCTGGCC+AGG 0.474048 1_4:+64405557 None:intergenic
AGTCTCGACCACCATGGGCT+TGG 0.475046 1_4:+64405385 None:intergenic
AGGCTCATTGATTTGGTCAA+GGG 0.475242 1_4:+64405913 None:intergenic
TGTAAAGTTAGCAGCCTCCT+TGG 0.477279 1_4:+64405604 None:intergenic
AGCTTGTTACTAAGATTGAT+AGG 0.481965 1_4:-64405484 Msa0177450:CDS
ACAATCAGCTTGGGATGTTC+CGG 0.483792 1_4:+64406355 None:intergenic
GACGAGAAATAGAAATACTA+AGG 0.487881 1_4:+64405156 None:intergenic
TCATCCTATTTGAAGAAGGT+TGG 0.491235 1_4:-64406046 Msa0177450:CDS
CTTGTACCAGTCAAGGTTTG+TGG 0.492698 1_4:+64405955 None:intergenic
GAGAATAGAGAGGGACGGTA+GGG 0.495192 1_4:+64408004 None:intergenic
ATCTCCCTCAAAACCAGAGA+TGG 0.496573 1_4:+64405994 None:intergenic
GGGTTTATAAACTAGTACTC+TGG 0.498858 1_4:+64408047 None:intergenic
CATCAAGGCTGTGGAGAAGA+AGG 0.499167 1_4:-64405294 Msa0177450:CDS
GAAACAGAGTCAGCAAGTCC+TGG 0.500128 1_4:+64404963 None:intergenic
CTGACCATCCTTAGAAATAC+CGG 0.501461 1_4:+64406292 None:intergenic
AGTTTCATCCTATTTGAAGA+AGG 0.501588 1_4:-64406050 Msa0177450:CDS
ATCATTGATTCCACTACTGG+TGG 0.502099 1_4:-64406323 Msa0177450:CDS
TAAAATTTGGATAAAATGAT+CGG 0.505831 1_4:+64404814 None:intergenic
CTTCAGTTGTCAGTCCAGTA+GGG 0.506099 1_4:+64405762 None:intergenic
GTTGTGTCTAGTCAGAAACT+TGG 0.507294 1_4:-64405009 Msa0177450:three_prime_UTR
AATTTCATCATATCTACCCT+TGG 0.508007 1_4:+64406081 None:intergenic
AATGACAGTGCAGTAGTACT+TGG 0.509426 1_4:+64406418 None:intergenic
AGCAGGATGATCTATCTACC+TGG 0.510712 1_4:-64405217 Msa0177450:three_prime_UTR
CATTCCTTGCTACTCGACTA+GGG 0.514565 1_4:-64407973 Msa0177450:five_prime_UTR
TGAGAACGGCACAATCAGCT+TGG 0.516820 1_4:+64406345 None:intergenic
AGGAATTGGAACTGTGCCTG+TGG 0.517140 1_4:-64405834 Msa0177450:CDS
TGAGAATAGAGAGGGACGGT+AGG 0.520249 1_4:+64408003 None:intergenic
GAAGAAGAAGTGAACCGAGC+AGG 0.523086 1_4:-64405234 Msa0177450:exon
CAGGATGTCTACAAGATTGG+AGG 0.523217 1_4:-64405854 Msa0177450:CDS
GAACAAAAGCTAAGAGATGA+AGG 0.523844 1_4:+64405095 None:intergenic
CTTAAGGCTGAACGGGAAAG+AGG 0.523952 1_4:-64406479 Msa0177450:CDS
GAGCCCAAATTCTTGAAGAA+TGG 0.528480 1_4:-64405437 Msa0177450:CDS
ATGCGTCAAACTGTAGCTGT+TGG 0.528651 1_4:-64405320 Msa0177450:CDS
TCCCACATTGTCACCAGGAA+GGG 0.528721 1_4:+64405700 None:intergenic
AGTCGGAGGGGCTTGTCTGA+AGG 0.529275 1_4:+64405884 None:intergenic
GAGAACGGCACAATCAGCTT+GGG 0.529995 1_4:+64406346 None:intergenic
GATTGATAGGCGTTCCGGTA+AGG 0.530821 1_4:-64405471 Msa0177450:CDS
CTTCCAAAGAGCAATGTCAA+TGG 0.534369 1_4:+64406451 None:intergenic
GGAATTGGAACTGTGCCTGT+GGG 0.536194 1_4:-64405833 Msa0177450:CDS
AGCAAACTTGACAGCAATGT+GGG 0.541075 1_4:+64405508 None:intergenic
TGTAGCTGTTGGGGTCATCA+AGG 0.544261 1_4:-64405309 Msa0177450:CDS
TGCACTGTCATTGATGCCCC+CGG 0.544718 1_4:-64406407 Msa0177450:CDS
AAATGATCGGTACTTTGCGC+AGG 0.544963 1_4:+64404827 None:intergenic
CACTTGATCTACAAGCTTGG+AGG 0.545798 1_4:-64406590 Msa0177450:CDS
GTCTGCTTAAGTCCTCCACT+GGG 0.549538 1_4:+64405269 None:intergenic
GAGAAAGTCTCGACCACCAT+GGG 0.551044 1_4:+64405380 None:intergenic
GGCTTCAAAACCACCAGTAG+TGG 0.551737 1_4:+64406313 None:intergenic
GCATGTCTCTAACGGCAAAA+CGG 0.555523 1_4:+64405339 None:intergenic
GGTCACTTGATCTACAAGCT+TGG 0.555547 1_4:-64406593 Msa0177450:CDS
TCAAGGGCCTCAAGAAGGGT+TGG 0.557590 1_4:+64405929 None:intergenic
TTGGTCAAGGGCCTCAAGAA+GGG 0.558003 1_4:+64405925 None:intergenic
TTGGACCCTTGTACCAGTCA+AGG 0.558366 1_4:+64405948 None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG 0.559179 1_4:+64406267 None:intergenic
TCATTGGCCATGTCGACTCT+GGG 0.559180 1_4:-64406631 Msa0177450:CDS
AGCGCTCCACAAACCTTGAC+TGG 0.559246 1_4:-64405961 Msa0177450:CDS
AGGTGTGGCAGTCAAGAACA+GGG 0.559985 1_4:+64405531 None:intergenic
ATAACTATGATATGCTTCCC+AGG 0.560332 1_4:+64405199 None:intergenic
TTTGGTCAAGGGCCTCAAGA+AGG 0.560467 1_4:+64405924 None:intergenic
TTCTTGAAGAATGGTGATGC+TGG 0.561159 1_4:-64405428 Msa0177450:CDS
AGTTAGCAGCCTCCTTGGCT+GGG 0.563218 1_4:+64405609 None:intergenic
GAAGCCGGTATTTCTAAGGA+TGG 0.564413 1_4:-64406296 Msa0177450:CDS
TCTGGGAAATCAACCACCAC+TGG 0.564990 1_4:-64406614 Msa0177450:CDS
TCGACCACCATGGGCTTGGT+GGG 0.567383 1_4:+64405389 None:intergenic
ACAAACCTTGACTGGTACAA+GGG 0.567625 1_4:-64405953 Msa0177450:CDS
GGTTGTTACCTCGTGAAGAA+GGG 0.568985 1_4:+64407929 None:intergenic
TAATGAAATCCCTGTGTCCG+GGG 0.570266 1_4:+64406390 None:intergenic
TTCATTAAGAACATGATCAC+CGG 0.572337 1_4:-64406374 Msa0177450:CDS
AGCAAGGAGAGCATGTTCAC+GGG 0.572470 1_4:+64406268 None:intergenic
CTTAAGCTTGTCAAGGACCC+AGG 0.575518 1_4:+64406496 None:intergenic
ATCCTGAAGTGGTAGTCGGA+GGG 0.576252 1_4:+64405871 None:intergenic
CCAGTTTCAACACGCCCCAC+AGG 0.580692 1_4:+64405818 None:intergenic
TATCTACCCTTGGAGTACTT+GGG 0.580849 1_4:+64406091 None:intergenic
GCGTCAAACTGTAGCTGTTG+GGG 0.581681 1_4:-64405318 Msa0177450:CDS
ATCCCACATTGTCACCAGGA+AGG 0.585536 1_4:+64405699 None:intergenic
GGCCCTTCCTGGTGACAATG+TGG 0.586985 1_4:-64405702 Msa0177450:CDS
TTGATTTCCCAGAGTCGACA+TGG 0.588973 1_4:+64406624 None:intergenic
CTGTTAAGGATCTCAAGCGT+GGG 0.589274 1_4:-64405658 Msa0177450:CDS
TTCTCTGAATATCCTCCACT+CGG 0.591172 1_4:-64405362 Msa0177450:CDS
GCCCTTCCTGGTGACAATGT+GGG 0.592402 1_4:-64405701 Msa0177450:CDS
TTGACAAGCTTAAGGCTGAA+CGG 0.594016 1_4:-64406487 Msa0177450:CDS
TGTTGGGGTCATCAAGGCTG+TGG 0.596509 1_4:-64405303 Msa0177450:CDS
TGGTGTCATAAAACCTGGAA+TGG 0.598505 1_4:-64405798 Msa0177450:CDS
TAGATATGATGAAATTGTGA+AGG 0.599770 1_4:-64406074 Msa0177450:CDS
CTCCAAGGATGACCCAGCCA+AGG 0.602045 1_4:-64405621 Msa0177450:CDS
AGTTTGACGCATGTCTCTAA+CGG 0.603969 1_4:+64405331 None:intergenic
GCGTTCATTCAAGTATGCCT+GGG 0.605879 1_4:-64406513 Msa0177450:CDS
TCTCCCTCAAAACCAGAGAT+GGG 0.607478 1_4:+64405995 None:intergenic
TCCTGAAGTGGTAGTCGGAG+GGG 0.608492 1_4:+64405872 None:intergenic
GATGATTCCCACCAAGCCCA+TGG 0.611182 1_4:-64405396 Msa0177450:CDS
ACGGCAAAACGGCCGAGTGG+AGG 0.611940 1_4:+64405350 None:intergenic
TACTACACCCAAGTACTCCA+AGG 0.613862 1_4:-64406098 Msa0177450:CDS
GTTCAGCCTTAAGCTTGTCA+AGG 0.618150 1_4:+64406489 None:intergenic
CAAGGGTCCAACCCTTCTTG+AGG 0.620566 1_4:-64405936 Msa0177450:CDS
TCACACCAAGAGTGAAAGCA+AGG 0.621193 1_4:+64406252 None:intergenic
ATCTTGTAGACATCCTGAAG+TGG 0.621388 1_4:+64405860 None:intergenic
GCTGTTAAGGATCTCAAGCG+TGG 0.622485 1_4:-64405659 Msa0177450:CDS
TTCCGGTAAGGAAATTGAGA+AGG 0.622677 1_4:-64405459 Msa0177450:CDS
TTCAGTTGTCAGTCCAGTAG+GGG 0.622733 1_4:+64405763 None:intergenic
AGCCATTTCCAATCTGGCCA+GGG 0.623271 1_4:+64405558 None:intergenic
GAAACTGGTGTCATAAAACC+TGG 0.623591 1_4:-64405803 Msa0177450:CDS
GACAACTGAAGTCAAGTCTG+TGG 0.624055 1_4:-64405750 Msa0177450:CDS
GCAGGATGATCTATCTACCT+GGG 0.625090 1_4:-64405216 Msa0177450:three_prime_UTR
TTGACAAGCGTGTCATCGAG+AGG 0.625348 1_4:-64406565 Msa0177450:CDS
GTATGTTGCCTCAAACTCCA+AGG 0.633897 1_4:-64405636 Msa0177450:CDS
CATCCTGAAGTGGTAGTCGG+AGG 0.637451 1_4:+64405870 None:intergenic
AGACATCCTGAAGTGGTAGT+CGG 0.638051 1_4:+64405867 None:intergenic
CAGCAAACTTGACAGCAATG+TGG 0.638328 1_4:+64405507 None:intergenic
AGAACTGGGTTCTAGATCGA+CGG 0.639454 1_4:-64405070 Msa0177450:three_prime_UTR
GGTAGATAGATCATCCTGCT+CGG 0.643375 1_4:+64405220 None:intergenic
GAATTGGAACTGTGCCTGTG+GGG 0.644316 1_4:-64405832 Msa0177450:CDS
AGTGGAATCAATGATGAGAA+CGG 0.644693 1_4:+64406331 None:intergenic
TGACAGCAATGTGGGAGGTG+TGG 0.650950 1_4:+64405516 None:intergenic
GAAACCCTAGTCGAGTAGCA+AGG 0.651284 1_4:+64407969 None:intergenic
AGAGAAAGTCTCGACCACCA+TGG 0.654922 1_4:+64405379 None:intergenic
TTAAATCCCACATTGTCACC+AGG 0.657091 1_4:+64405695 None:intergenic
AATGATCTGCTGTTGCAACA+AGG 0.659392 1_4:-64406225 Msa0177450:intron
CAAGGATGACCCAGCCAAGG+AGG 0.659665 1_4:-64405618 Msa0177450:CDS
AATGAAATCCCTGTGTCCGG+GGG 0.661737 1_4:+64406391 None:intergenic
GAGAAGAAGGATCCCAGTGG+AGG 0.667988 1_4:-64405281 Msa0177450:CDS
AAAGGTTCACATCAACATTG+TGG 0.675059 1_4:-64406654 Msa0177450:CDS
ACTACACCCAAGTACTCCAA+GGG 0.678033 1_4:-64406097 Msa0177450:CDS
CTTGTAGATCAAGTGACCAG+TGG 0.682705 1_4:+64406598 None:intergenic
CTAACGGCAAAACGGCCGAG+TGG 0.689978 1_4:+64405347 None:intergenic
AAACTTGACAGCAATGTGGG+AGG 0.695398 1_4:+64405511 None:intergenic
GTAGATCAAGTGACCAGTGG+TGG 0.699002 1_4:+64406601 None:intergenic
GTGATCATAATGAATCACCC+TGG 0.699056 1_4:-64405575 Msa0177450:CDS
GGTGTGGCAGTCAAGAACAG+GGG 0.711459 1_4:+64405532 None:intergenic
GATTCCCACCAAGCCCATGG+TGG 0.723849 1_4:-64405393 Msa0177450:CDS
AAAGCTTAAACATCACACCA+AGG 0.724807 1_4:+64404909 None:intergenic
ACTGGGTTCTAGATCGACGG+TGG 0.727144 1_4:-64405067 Msa0177450:three_prime_UTR
GTGGAGAAGAAGGATCCCAG+TGG 0.734762 1_4:-64405284 Msa0177450:CDS
GCATCATGAAGCTCTCACAG+AGG 0.758446 1_4:-64405723 Msa0177450:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTCATTTTTTTTTTTACTT+TGG - chr1_4:64405864-64405883 Msa0177450:CDS 10.0%
!!! ATTTAATTTTTTTCTTTCTT+TGG - chr1_4:64406112-64406131 Msa0177450:intron 10.0%
!!! TTTAATTTTTTTCTTTCTTT+GGG - chr1_4:64406113-64406132 Msa0177450:intron 10.0%
!!! TATGAAATTTAGTTTCAATT+TGG - chr1_4:64405362-64405381 Msa0177450:CDS 15.0%
!!! AATCCTTTATGTTTTTAAAT+AGG - chr1_4:64405456-64405475 Msa0177450:CDS 15.0%
!! ATATAGAACACAAAAAAAAA+GGG + chr1_4:64405825-64405844 None:intergenic 15.0%
!! AATATAGAACACAAAAAAAA+AGG + chr1_4:64405826-64405845 None:intergenic 15.0%
!!! TTAATTTTTTTCTTTCTTTG+GGG - chr1_4:64406114-64406133 Msa0177450:intron 15.0%
!! ATCTTGAATTCATTTATTTA+TGG - chr1_4:64406709-64406728 Msa0177450:intron 15.0%
!! ATCATAGTTATTACAATAAA+TGG - chr1_4:64407676-64407695 Msa0177450:intron 15.0%
!! TAAAATTTGGATAAAATGAT+CGG + chr1_4:64408052-64408071 None:intergenic 15.0%
!!! TGTTATATACTTCTTTTGTT+TGG - chr1_4:64405250-64405269 Msa0177450:CDS 20.0%
!! TAAATTTCATAACGTAAAAC+AGG + chr1_4:64405353-64405372 None:intergenic 20.0%
!! ATTCAGAATTACAATTAAAC+AGG + chr1_4:64405422-64405441 None:intergenic 20.0%
!! TTATTACAATAAATGGTCTT+TGG - chr1_4:64407683-64407702 Msa0177450:intron 20.0%
!!! TTATTTTTGTGTTTATTCCT+TGG - chr1_4:64407937-64407956 Msa0177450:intron 20.0%
!! TTTTAATATAAGGGTTTCAG+GGG + chr1_4:64404839-64404858 None:intergenic 25.0%
!!! GTTTTAATATAAGGGTTTCA+GGG + chr1_4:64404840-64404859 None:intergenic 25.0%
!!! AAACGAAATTGATGGATTTA+GGG + chr1_4:64404958-64404977 None:intergenic 25.0%
! CATATCATCAATTCATTCAT+AGG - chr1_4:64405000-64405019 Msa0177450:three_prime_UTR 25.0%
! CTGATAAATAAAAGAACGAT+TGG + chr1_4:64405124-64405143 None:intergenic 25.0%
! ACGTAAGGATGATATAATAA+TGG + chr1_4:64405208-64405227 None:intergenic 25.0%
!!! CTGTTTTAGTGTAATTACAT+AGG - chr1_4:64405310-64405329 Msa0177450:CDS 25.0%
!! TACATAGGATCATTTGAAAT+TGG - chr1_4:64405325-64405344 Msa0177450:CDS 25.0%
!! TAAAGGATTATCACACTTTT+TGG + chr1_4:64405445-64405464 None:intergenic 25.0%
! CCACCTATTTAAAAACATAA+AGG + chr1_4:64405462-64405481 None:intergenic 25.0%
!!! CCTTTATGTTTTTAAATAGG+TGG - chr1_4:64405459-64405478 Msa0177450:CDS 25.0%
!!! TTGTGAAGGTTTTAACTTTT+TGG - chr1_4:64405492-64405511 Msa0177450:CDS 25.0%
! AGTTAGTAGTTATTTGTTTC+TGG - chr1_4:64405583-64405602 Msa0177450:CDS 25.0%
! TCTGATCTTATCTATTAATC+AGG + chr1_4:64405723-64405742 None:intergenic 25.0%
! CAAAATGTTATCCCATAAAT+GGG + chr1_4:64406000-64406019 None:intergenic 25.0%
! ACAAAATGTTATCCCATAAA+TGG + chr1_4:64406001-64406020 None:intergenic 25.0%
! TAGATATGATGAAATTGTGA+AGG - chr1_4:64406789-64406808 Msa0177450:intron 25.0%
!! GGTTTTAATATAAGGGTTTC+AGG + chr1_4:64404841-64404860 None:intergenic 30.0%
!! ACGGTAGGGTTTTAATATAA+GGG + chr1_4:64404848-64404867 None:intergenic 30.0%
!! GAGAATTTTGAGAATAGAGA+GGG + chr1_4:64404871-64404890 None:intergenic 30.0%
!! TGAGAATTTTGAGAATAGAG+AGG + chr1_4:64404872-64404891 None:intergenic 30.0%
!! GAAACGAAATTGATGGATTT+AGG + chr1_4:64404959-64404978 None:intergenic 30.0%
TCGATAAGAAACGAAATTGA+TGG + chr1_4:64404966-64404985 None:intergenic 30.0%
!! TTTTATTTATCAGTCATCGC+TGG - chr1_4:64405131-64405150 Msa0177450:three_prime_UTR 30.0%
AGGATGATATAATAATGGCA+TGG + chr1_4:64405203-64405222 None:intergenic 30.0%
CTCAAAAAAAGAACAACGTA+AGG + chr1_4:64405223-64405242 None:intergenic 30.0%
!! TTCTTTTGTTTGGTATTCCT+TGG - chr1_4:64405260-64405279 Msa0177450:CDS 30.0%
TTGCAAAAAAACAGAATCCA+AGG + chr1_4:64405280-64405299 None:intergenic 30.0%
ATGAAATATGATGTTTCAGC+AGG + chr1_4:64405611-64405630 None:intergenic 30.0%
! TGCTCATGTATTATTTTCCT+TGG - chr1_4:64406140-64406159 Msa0177450:intron 30.0%
! TTTGTGGTTTTCAGCTTTTA+AGG - chr1_4:64406171-64406190 Msa0177450:intron 30.0%
!! TTTCAGCTTTTAAGGACTAA+TGG - chr1_4:64406179-64406198 Msa0177450:intron 30.0%
!! TTCAGCTTTTAAGGACTAAT+GGG - chr1_4:64406180-64406199 Msa0177450:intron 30.0%
TTCATTAAGAACATGATCAC+CGG - chr1_4:64406489-64406508 Msa0177450:CDS 30.0%
AATTTCATCATATCTACCCT+TGG + chr1_4:64406785-64406804 None:intergenic 30.0%
AGTTTCATCCTATTTGAAGA+AGG - chr1_4:64406813-64406832 Msa0177450:intron 30.0%
! GAACAAATGGAATTTTGTCT+GGG + chr1_4:64406849-64406868 None:intergenic 30.0%
!! AGCTTGTTACTAAGATTGAT+AGG - chr1_4:64407379-64407398 Msa0177450:intron 30.0%
TCACCATTCTTCAAGAATTT+GGG + chr1_4:64407432-64407451 None:intergenic 30.0%
ATCACCATTCTTCAAGAATT+TGG + chr1_4:64407433-64407452 None:intergenic 30.0%
GACGAGAAATAGAAATACTA+AGG + chr1_4:64407710-64407729 None:intergenic 30.0%
TATGGTTTAGGCAATATATC+AGG - chr1_4:64407995-64408014 Msa0177450:five_prime_UTR 30.0%
GGGTTTATAAACTAGTACTC+TGG + chr1_4:64404819-64404838 None:intergenic 35.0%
!! GACGGTAGGGTTTTAATATA+AGG + chr1_4:64404849-64404868 None:intergenic 35.0%
!! ATTTTGAGAATAGAGAGGGA+CGG + chr1_4:64404867-64404886 None:intergenic 35.0%
!! GTGGTTTTACTTTGTTGTGA+AGG - chr1_4:64405478-64405497 Msa0177450:CDS 35.0%
ACAACAACAATATGTCAGCA+GGG + chr1_4:64405635-64405654 None:intergenic 35.0%
AACAACAACAATATGTCAGC+AGG + chr1_4:64405636-64405655 None:intergenic 35.0%
GATTCTTCAGCAGATATAAC+AGG + chr1_4:64405690-64405709 None:intergenic 35.0%
ACAAAAAAAAAGGGACTCCA+CGG + chr1_4:64405816-64405835 None:intergenic 35.0%
! CATCAGTCTTGTAAAACTGA+AGG - chr1_4:64405924-64405943 Msa0177450:CDS 35.0%
ATTGTTGCCAAACCCATTTA+TGG - chr1_4:64405985-64406004 Msa0177450:CDS 35.0%
TTGTTGCCAAACCCATTTAT+GGG - chr1_4:64405986-64406005 Msa0177450:CDS 35.0%
! TGTTATCCCATAAATGGGTT+TGG + chr1_4:64405995-64406014 None:intergenic 35.0%
!! CTTTTAAGGACTAATGGGTA+AGG - chr1_4:64406185-64406204 Msa0177450:intron 35.0%
AAAGGTTCACATCAACATTG+TGG - chr1_4:64406209-64406228 Msa0177450:intron 35.0%
AGTGGAATCAATGATGAGAA+CGG + chr1_4:64406535-64406554 None:intergenic 35.0%
!!! TTTTGAAGCCGGTATTTCTA+AGG - chr1_4:64406563-64406582 Msa0177450:CDS 35.0%
ATCCATCTGTTCAAGATAGA+TGG + chr1_4:64406743-64406762 None:intergenic 35.0%
TCATCCTATTTGAAGAAGGT+TGG - chr1_4:64406817-64406836 Msa0177450:intron 35.0%
ATAGCCAACCTTCTTCAAAT+AGG + chr1_4:64406824-64406843 None:intergenic 35.0%
! GGAACAAATGGAATTTTGTC+TGG + chr1_4:64406850-64406869 None:intergenic 35.0%
! TGTCAAGAATGTTGCTGTTA+AGG - chr1_4:64407191-64407210 Msa0177450:intron 35.0%
ATAACTATGATATGCTTCCC+AGG + chr1_4:64407667-64407686 None:intergenic 35.0%
GAACAAAAGCTAAGAGATGA+AGG + chr1_4:64407771-64407790 None:intergenic 35.0%
!! TTAGCTTTTGTTCTCAGAAC+TGG - chr1_4:64407778-64407797 Msa0177450:intron 35.0%
! TAGCTTTTGTTCTCAGAACT+GGG - chr1_4:64407779-64407798 Msa0177450:intron 35.0%
AAAGCTTAAACATCACACCA+AGG + chr1_4:64407957-64407976 None:intergenic 35.0%
! TGTGATGTTTAAGCTTTTGC+TGG - chr1_4:64407960-64407979 Msa0177450:five_prime_UTR 35.0%
! GTGATGTTTAAGCTTTTGCT+GGG - chr1_4:64407961-64407980 Msa0177450:five_prime_UTR 35.0%
! TGATGTTTAAGCTTTTGCTG+GGG - chr1_4:64407962-64407981 Msa0177450:five_prime_UTR 35.0%
CAACAACAATATGTCAGCAG+GGG + chr1_4:64405634-64405653 None:intergenic 40.0%
TACTCTACCACCGAGTTAAA+GGG + chr1_4:64405790-64405809 None:intergenic 40.0%
TTACTCTACCACCGAGTTAA+AGG + chr1_4:64405791-64405810 None:intergenic 40.0%
! GTCAACAACACACTGACTAA+AGG + chr1_4:64406081-64406100 None:intergenic 40.0%
! TTAGTCAGTGTGTTGTTGAC+AGG - chr1_4:64406081-64406100 Msa0177450:CDS 40.0%
! TTCCTTGGCAGATCTTTTTG+TGG - chr1_4:64406155-64406174 Msa0177450:intron 40.0%
AACCACAAAAAGATCTGCCA+AGG + chr1_4:64406160-64406179 None:intergenic 40.0%
! AGGACTAATGGGTAAGGAAA+AGG - chr1_4:64406191-64406210 Msa0177450:intron 40.0%
CACATCAACATTGTGGTCAT+TGG - chr1_4:64406216-64406235 Msa0177450:intron 40.0%
TTGACAAGCTTAAGGCTGAA+CGG - chr1_4:64406376-64406395 Msa0177450:CDS 40.0%
TCACCATTGACATTGCTCTT+TGG - chr1_4:64406409-64406428 Msa0177450:CDS 40.0%
CTTCCAAAGAGCAATGTCAA+TGG + chr1_4:64406415-64406434 None:intergenic 40.0%
AATGACAGTGCAGTAGTACT+TGG + chr1_4:64406448-64406467 None:intergenic 40.0%
TTAATGAAATCCCTGTGTCC+GGG + chr1_4:64406477-64406496 None:intergenic 40.0%
CTTAATGAAATCCCTGTGTC+CGG + chr1_4:64406478-64406497 None:intergenic 40.0%
!! CTCATCATTGATTCCACTAC+TGG - chr1_4:64406537-64406556 Msa0177450:CDS 40.0%
!! ATCATTGATTCCACTACTGG+TGG - chr1_4:64406540-64406559 Msa0177450:CDS 40.0%
CTGACCATCCTTAGAAATAC+CGG + chr1_4:64406574-64406593 None:intergenic 40.0%
AATGATCTGCTGTTGCAACA+AGG - chr1_4:64406638-64406657 Msa0177450:CDS 40.0%
TGCCATCTATCTTGAACAGA+TGG - chr1_4:64406738-64406757 Msa0177450:intron 40.0%
! TATCTACCCTTGGAGTACTT+GGG + chr1_4:64406775-64406794 None:intergenic 40.0%
! ATATCTACCCTTGGAGTACT+TGG + chr1_4:64406776-64406795 None:intergenic 40.0%
ATTCCATTTGTTCCCATCTC+TGG - chr1_4:64406856-64406875 Msa0177450:intron 40.0%
AAACCAGAGATGGGAACAAA+TGG + chr1_4:64406862-64406881 None:intergenic 40.0%
ACAAACCTTGACTGGTACAA+GGG - chr1_4:64406910-64406929 Msa0177450:intron 40.0%
!! AGGCTCATTGATTTGGTCAA+GGG + chr1_4:64406953-64406972 None:intergenic 40.0%
!! TAGGCTCATTGATTTGGTCA+AGG + chr1_4:64406954-64406973 None:intergenic 40.0%
ACCAAATCAATGAGCCTAAG+AGG - chr1_4:64406956-64406975 Msa0177450:intron 40.0%
ATCTTGTAGACATCCTGAAG+TGG + chr1_4:64407006-64407025 None:intergenic 40.0%
CTTCAGGATGTCTACAAGAT+TGG - chr1_4:64407006-64407025 Msa0177450:intron 40.0%
GTCTACAAGATTGGAGGAAT+TGG - chr1_4:64407015-64407034 Msa0177450:intron 40.0%
!! GAAACTGGTGTCATAAAACC+TGG - chr1_4:64407060-64407079 Msa0177450:intron 40.0%
!! TGGTGTCATAAAACCTGGAA+TGG - chr1_4:64407065-64407084 Msa0177450:intron 40.0%
TTAAATCCCACATTGTCACC+AGG + chr1_4:64407171-64407190 None:intergenic 40.0%
GTGATCATAATGAATCACCC+TGG - chr1_4:64407288-64407307 Msa0177450:intron 40.0%
AGCAAACTTGACAGCAATGT+GGG + chr1_4:64407358-64407377 None:intergenic 40.0%
ACTAAGATTGATAGGCGTTC+CGG - chr1_4:64407387-64407406 Msa0177450:intron 40.0%
TTCCGGTAAGGAAATTGAGA+AGG - chr1_4:64407404-64407423 Msa0177450:intron 40.0%
CTCCTTCTCAATTTCCTTAC+CGG + chr1_4:64407409-64407428 None:intergenic 40.0%
GAGCCCAAATTCTTGAAGAA+TGG - chr1_4:64407426-64407445 Msa0177450:intron 40.0%
! TTCTTGAAGAATGGTGATGC+TGG - chr1_4:64407435-64407454 Msa0177450:intron 40.0%
TTCTCTGAATATCCTCCACT+CGG - chr1_4:64407501-64407520 Msa0177450:intron 40.0%
AGTTTGACGCATGTCTCTAA+CGG + chr1_4:64407535-64407554 None:intergenic 40.0%
! CTTCTTCTTCAATGCTGACT+TGG + chr1_4:64407619-64407638 None:intergenic 40.0%
GTTGTGTCTAGTCAGAAACT+TGG - chr1_4:64407854-64407873 Msa0177450:intron 40.0%
! GGCATGGTTCTATATGGTTT+AGG - chr1_4:64407983-64408002 Msa0177450:five_prime_UTR 40.0%
TCATTCCTTGCTACTCGACT+AGG - chr1_4:64404889-64404908 Msa0177450:three_prime_UTR 45.0%
CATTCCTTGCTACTCGACTA+GGG - chr1_4:64404890-64404909 Msa0177450:three_prime_UTR 45.0%
ACCTCGTGAAGAAGGGAAAA+AGG + chr1_4:64404930-64404949 None:intergenic 45.0%
GGTTGTTACCTCGTGAAGAA+GGG + chr1_4:64404937-64404956 None:intergenic 45.0%
!!! TCAGTCATCGCTGGTTTTGA+AGG - chr1_4:64405140-64405159 Msa0177450:three_prime_UTR 45.0%
!!! ATCGCTGGTTTTGAAGGAGT+CGG - chr1_4:64405146-64405165 Msa0177450:three_prime_UTR 45.0%
GACACACACATATGCTTGTC+CGG - chr1_4:64405746-64405765 Msa0177450:CDS 45.0%
ACACACACATATGCTTGTCC+GGG - chr1_4:64405747-64405766 Msa0177450:CDS 45.0%
TCAGATGGAGCCCTTTAACT+CGG - chr1_4:64405777-64405796 Msa0177450:CDS 45.0%
AACTTGCCCTTATAGCTCAG+TGG - chr1_4:64405896-64405915 Msa0177450:CDS 45.0%
GCTCTACCACTGAGCTATAA+GGG + chr1_4:64405905-64405924 None:intergenic 45.0%
TGCTCTACCACTGAGCTATA+AGG + chr1_4:64405906-64405925 None:intergenic 45.0%
TTGATTTCCCAGAGTCGACA+TGG + chr1_4:64406242-64406261 None:intergenic 45.0%
CTTGTAGATCAAGTGACCAG+TGG + chr1_4:64406268-64406287 None:intergenic 45.0%
GGTCACTTGATCTACAAGCT+TGG - chr1_4:64406270-64406289 Msa0177450:CDS 45.0%
CACTTGATCTACAAGCTTGG+AGG - chr1_4:64406273-64406292 Msa0177450:CDS 45.0%
AGCGTTCATTCAAGTATGCC+TGG - chr1_4:64406349-64406368 Msa0177450:CDS 45.0%
GCGTTCATTCAAGTATGCCT+GGG - chr1_4:64406350-64406369 Msa0177450:CDS 45.0%
GTTCAGCCTTAAGCTTGTCA+AGG + chr1_4:64406377-64406396 None:intergenic 45.0%
TGACAAGCTTAAGGCTGAAC+GGG - chr1_4:64406377-64406396 Msa0177450:CDS 45.0%
TAATGAAATCCCTGTGTCCG+GGG + chr1_4:64406476-64406495 None:intergenic 45.0%
ACAATCAGCTTGGGATGTTC+CGG + chr1_4:64406511-64406530 None:intergenic 45.0%
!!! ACTACTGGTGGTTTTGAAGC+CGG - chr1_4:64406552-64406571 Msa0177450:CDS 45.0%
! GAAGCCGGTATTTCTAAGGA+TGG - chr1_4:64406567-64406586 Msa0177450:CDS 45.0%
! AAGCAAGGAGAGCATGTTCA+CGG + chr1_4:64406599-64406618 None:intergenic 45.0%
TCACACCAAGAGTGAAAGCA+AGG + chr1_4:64406614-64406633 None:intergenic 45.0%
TACTACACCCAAGTACTCCA+AGG - chr1_4:64406765-64406784 Msa0177450:intron 45.0%
ACTACACCCAAGTACTCCAA+GGG - chr1_4:64406766-64406785 Msa0177450:intron 45.0%
!!! TGTTCCCATCTCTGGTTTTG+AGG - chr1_4:64406864-64406883 Msa0177450:intron 45.0%
!!! GTTCCCATCTCTGGTTTTGA+GGG - chr1_4:64406865-64406884 Msa0177450:intron 45.0%
TCTCCCTCAAAACCAGAGAT+GGG + chr1_4:64406871-64406890 None:intergenic 45.0%
ATCTCCCTCAAAACCAGAGA+TGG + chr1_4:64406872-64406891 None:intergenic 45.0%
CTTGTACCAGTCAAGGTTTG+TGG + chr1_4:64406911-64406930 None:intergenic 45.0%
CACAAACCTTGACTGGTACA+AGG - chr1_4:64406909-64406928 Msa0177450:intron 45.0%
! GCCTCTTAGGCTCATTGATT+TGG + chr1_4:64406960-64406979 None:intergenic 45.0%
AGACATCCTGAAGTGGTAGT+CGG + chr1_4:64406999-64407018 None:intergenic 45.0%
CAGGATGTCTACAAGATTGG+AGG - chr1_4:64407009-64407028 Msa0177450:intron 45.0%
GCAAAAGTGACAACCATTCC+AGG + chr1_4:64407081-64407100 None:intergenic 45.0%
TTCAGTTGTCAGTCCAGTAG+GGG + chr1_4:64407103-64407122 None:intergenic 45.0%
CTTCAGTTGTCAGTCCAGTA+GGG + chr1_4:64407104-64407123 None:intergenic 45.0%
ACTTCAGTTGTCAGTCCAGT+AGG + chr1_4:64407105-64407124 None:intergenic 45.0%
GACAACTGAAGTCAAGTCTG+TGG - chr1_4:64407113-64407132 Msa0177450:intron 45.0%
CTGTTAAGGATCTCAAGCGT+GGG - chr1_4:64407205-64407224 Msa0177450:intron 45.0%
GTATGTTGCCTCAAACTCCA+AGG - chr1_4:64407227-64407246 Msa0177450:intron 45.0%
TGTAAAGTTAGCAGCCTCCT+TGG + chr1_4:64407262-64407281 None:intergenic 45.0%
AAACTTGACAGCAATGTGGG+AGG + chr1_4:64407355-64407374 None:intergenic 45.0%
CAGCAAACTTGACAGCAATG+TGG + chr1_4:64407359-64407378 None:intergenic 45.0%
GCATGTCTCTAACGGCAAAA+CGG + chr1_4:64407527-64407546 None:intergenic 45.0%
ATGCGTCAAACTGTAGCTGT+TGG - chr1_4:64407543-64407562 Msa0177450:intron 45.0%
TGCGTCAAACTGTAGCTGTT+GGG - chr1_4:64407544-64407563 Msa0177450:intron 45.0%
GGTAGATAGATCATCCTGCT+CGG + chr1_4:64407646-64407665 None:intergenic 45.0%
AGCAGGATGATCTATCTACC+TGG - chr1_4:64407646-64407665 Msa0177450:intron 45.0%
GCAGGATGATCTATCTACCT+GGG - chr1_4:64407647-64407666 Msa0177450:intron 45.0%
!! AGAACTGGGTTCTAGATCGA+CGG - chr1_4:64407793-64407812 Msa0177450:intron 45.0%
GTAAGCAAGCAGTAAGTTCC+AGG - chr1_4:64407882-64407901 Msa0177450:intron 45.0%
! TTTAAGCTTTTGCTGGGGCA+TGG - chr1_4:64407967-64407986 Msa0177450:five_prime_UTR 45.0%
AAATGATCGGTACTTTGCGC+AGG + chr1_4:64408039-64408058 None:intergenic 45.0%
GAGAATAGAGAGGGACGGTA+GGG + chr1_4:64404862-64404881 None:intergenic 50.0%
TGAGAATAGAGAGGGACGGT+AGG + chr1_4:64404863-64404882 None:intergenic 50.0%
GAAACCCTAGTCGAGTAGCA+AGG + chr1_4:64404897-64404916 None:intergenic 50.0%
! GCCTTTTTCCCTTCTTCACG+AGG - chr1_4:64404926-64404945 Msa0177450:three_prime_UTR 50.0%
GGGTTGTTACCTCGTGAAGA+AGG + chr1_4:64404938-64404957 None:intergenic 50.0%
GAAATGAATTGCCCTCACGC+AGG + chr1_4:64405963-64405982 None:intergenic 50.0%
!! TCATTGGCCATGTCGACTCT+GGG - chr1_4:64406232-64406251 Msa0177450:CDS 50.0%
TCTGGGAAATCAACCACCAC+TGG - chr1_4:64406249-64406268 Msa0177450:CDS 50.0%
GTAGATCAAGTGACCAGTGG+TGG + chr1_4:64406265-64406284 None:intergenic 50.0%
TTGACAAGCGTGTCATCGAG+AGG - chr1_4:64406298-64406317 Msa0177450:CDS 50.0%
CTTAAGCTTGTCAAGGACCC+AGG + chr1_4:64406370-64406389 None:intergenic 50.0%
CTGGGTCCTTGACAAGCTTA+AGG - chr1_4:64406368-64406387 Msa0177450:CDS 50.0%
CTTAAGGCTGAACGGGAAAG+AGG - chr1_4:64406384-64406403 Msa0177450:CDS 50.0%
AATGAAATCCCTGTGTCCGG+GGG + chr1_4:64406475-64406494 None:intergenic 50.0%
GAGAACGGCACAATCAGCTT+GGG + chr1_4:64406520-64406539 None:intergenic 50.0%
TGAGAACGGCACAATCAGCT+TGG + chr1_4:64406521-64406540 None:intergenic 50.0%
GGCTTCAAAACCACCAGTAG+TGG + chr1_4:64406553-64406572 None:intergenic 50.0%
! AGCAAGGAGAGCATGTTCAC+GGG + chr1_4:64406598-64406617 None:intergenic 50.0%
GCTCTCCTTGCTTTCACTCT+TGG - chr1_4:64406606-64406625 Msa0177450:CDS 50.0%
TTGGACCCTTGTACCAGTCA+AGG + chr1_4:64406918-64406937 None:intergenic 50.0%
TTGGTCAAGGGCCTCAAGAA+GGG + chr1_4:64406941-64406960 None:intergenic 50.0%
TTTGGTCAAGGGCCTCAAGA+AGG + chr1_4:64406942-64406961 None:intergenic 50.0%
ATCCTGAAGTGGTAGTCGGA+GGG + chr1_4:64406995-64407014 None:intergenic 50.0%
AGGAATTGGAACTGTGCCTG+TGG - chr1_4:64407029-64407048 Msa0177450:intron 50.0%
GGAATTGGAACTGTGCCTGT+GGG - chr1_4:64407030-64407049 Msa0177450:intron 50.0%
GAATTGGAACTGTGCCTGTG+GGG - chr1_4:64407031-64407050 Msa0177450:intron 50.0%
!!! GTTGTCACTTTTGCCCCTAC+TGG - chr1_4:64407087-64407106 Msa0177450:intron 50.0%
GCATCATGAAGCTCTCACAG+AGG - chr1_4:64407140-64407159 Msa0177450:intron 50.0%
TCCCACATTGTCACCAGGAA+GGG + chr1_4:64407166-64407185 None:intergenic 50.0%
ATCCCACATTGTCACCAGGA+AGG + chr1_4:64407167-64407186 None:intergenic 50.0%
GCTGTTAAGGATCTCAAGCG+TGG - chr1_4:64407204-64407223 Msa0177450:intron 50.0%
TGGGTCATCCTTGGAGTTTG+AGG + chr1_4:64407238-64407257 None:intergenic 50.0%
ATGAATCACCCTGGCCAGAT+TGG - chr1_4:64407297-64407316 Msa0177450:intron 50.0%
AGCCATTTCCAATCTGGCCA+GGG + chr1_4:64407308-64407327 None:intergenic 50.0%
TAGCCATTTCCAATCTGGCC+AGG + chr1_4:64407309-64407328 None:intergenic 50.0%
GGGCATAGCCATTTCCAATC+TGG + chr1_4:64407314-64407333 None:intergenic 50.0%
AGGTGTGGCAGTCAAGAACA+GGG + chr1_4:64407335-64407354 None:intergenic 50.0%
GATTGATAGGCGTTCCGGTA+AGG - chr1_4:64407392-64407411 Msa0177450:intron 50.0%
GAGAAAGTCTCGACCACCAT+GGG + chr1_4:64407486-64407505 None:intergenic 50.0%
AGAGAAAGTCTCGACCACCA+TGG + chr1_4:64407487-64407506 None:intergenic 50.0%
GCGTCAAACTGTAGCTGTTG+GGG - chr1_4:64407545-64407564 Msa0177450:intron 50.0%
TGTAGCTGTTGGGGTCATCA+AGG - chr1_4:64407554-64407573 Msa0177450:intron 50.0%
CATCAAGGCTGTGGAGAAGA+AGG - chr1_4:64407569-64407588 Msa0177450:intron 50.0%
GTCTGCTTAAGTCCTCCACT+GGG + chr1_4:64407597-64407616 None:intergenic 50.0%
GAAGAAGAAGTGAACCGAGC+AGG - chr1_4:64407629-64407648 Msa0177450:intron 50.0%
GAAACAGAGTCAGCAAGTCC+TGG + chr1_4:64407903-64407922 None:intergenic 50.0%
! TGCTGGGGCATGGTTCTATA+TGG - chr1_4:64407977-64407996 Msa0177450:five_prime_UTR 50.0%
AAGGGAAAAAGGCTGCGCAG+CGG + chr1_4:64404919-64404938 None:intergenic 55.0%
!!! GCTGGTTTTGAAGGAGTCGG+TGG - chr1_4:64405149-64405168 Msa0177450:three_prime_UTR 55.0%
TGTCCGGGACTTGACTCAGA+TGG - chr1_4:64405762-64405781 Msa0177450:CDS 55.0%
GCTCCATCTGAGTCAAGTCC+CGG + chr1_4:64405768-64405787 None:intergenic 55.0%
GATGGAGCCCTTTAACTCGG+TGG - chr1_4:64405780-64405799 Msa0177450:CDS 55.0%
TCGGTGGTAGAGTAACACCG+TGG - chr1_4:64405796-64405815 Msa0177450:CDS 55.0%
CCTCACGCAGGATCGAACTA+CGG + chr1_4:64405951-64405970 None:intergenic 55.0%
CGTAGTTCGATCCTGCGTGA+GGG - chr1_4:64405949-64405968 Msa0177450:CDS 55.0%
!! GTCATTGGCCATGTCGACTC+TGG - chr1_4:64406231-64406250 Msa0177450:CDS 55.0%
TGCACTGTCATTGATGCCCC+CGG - chr1_4:64406456-64406475 Msa0177450:CDS 55.0%
AGCGCTCCACAAACCTTGAC+TGG - chr1_4:64406902-64406921 Msa0177450:intron 55.0%
CAAGGGTCCAACCCTTCTTG+AGG - chr1_4:64406927-64406946 Msa0177450:intron 55.0%
TCAAGGGCCTCAAGAAGGGT+TGG + chr1_4:64406937-64406956 None:intergenic 55.0%
GGCTTGTCTGAAGGCCTCTT+AGG + chr1_4:64406973-64406992 None:intergenic 55.0%
TCCTGAAGTGGTAGTCGGAG+GGG + chr1_4:64406994-64407013 None:intergenic 55.0%
CATCCTGAAGTGGTAGTCGG+AGG + chr1_4:64406996-64407015 None:intergenic 55.0%
GCCCTTCCTGGTGACAATGT+GGG - chr1_4:64407162-64407181 Msa0177450:intron 55.0%
AGTTAGCAGCCTCCTTGGCT+GGG + chr1_4:64407257-64407276 None:intergenic 55.0%
AAGTTAGCAGCCTCCTTGGC+TGG + chr1_4:64407258-64407277 None:intergenic 55.0%
CACCCTGGCCAGATTGGAAA+TGG - chr1_4:64407303-64407322 Msa0177450:intron 55.0%
GGTGTGGCAGTCAAGAACAG+GGG + chr1_4:64407334-64407353 None:intergenic 55.0%
GAGGTGTGGCAGTCAAGAAC+AGG + chr1_4:64407336-64407355 None:intergenic 55.0%
TGACAGCAATGTGGGAGGTG+TGG + chr1_4:64407350-64407369 None:intergenic 55.0%
GATGATTCCCACCAAGCCCA+TGG - chr1_4:64407467-64407486 Msa0177450:intron 55.0%
TGTTGGGGTCATCAAGGCTG+TGG - chr1_4:64407560-64407579 Msa0177450:intron 55.0%
GTGGAGAAGAAGGATCCCAG+TGG - chr1_4:64407579-64407598 Msa0177450:intron 55.0%
GAGAAGAAGGATCCCAGTGG+AGG - chr1_4:64407582-64407601 Msa0177450:intron 55.0%
GGTCTGCTTAAGTCCTCCAC+TGG + chr1_4:64407598-64407617 None:intergenic 55.0%
!! ACTGGGTTCTAGATCGACGG+TGG - chr1_4:64407796-64407815 Msa0177450:intron 55.0%
CCGTAGTTCGATCCTGCGTG+AGG - chr1_4:64405948-64405967 Msa0177450:CDS 60.0%
TCATTGATGCCCCCGGACAC+AGG - chr1_4:64406463-64406482 Msa0177450:CDS 60.0%
CATTGATGCCCCCGGACACA+GGG - chr1_4:64406464-64406483 Msa0177450:CDS 60.0%
AGTCGGAGGGGCTTGTCTGA+AGG + chr1_4:64406982-64407001 None:intergenic 60.0%
CCAGTTTCAACACGCCCCAC+AGG + chr1_4:64407048-64407067 None:intergenic 60.0%
CCTGTGGGGCGTGTTGAAAC+TGG - chr1_4:64407045-64407064 Msa0177450:intron 60.0%
GGCCCTTCCTGGTGACAATG+TGG - chr1_4:64407161-64407180 Msa0177450:intron 60.0%
CTCCAAGGATGACCCAGCCA+AGG - chr1_4:64407242-64407261 Msa0177450:intron 60.0%
CTCCTTGGCTGGGTCATCCT+TGG + chr1_4:64407247-64407266 None:intergenic 60.0%
CAAGGATGACCCAGCCAAGG+AGG - chr1_4:64407245-64407264 Msa0177450:intron 60.0%
GATTCCCACCAAGCCCATGG+TGG - chr1_4:64407470-64407489 Msa0177450:intron 60.0%
! TCGACCACCATGGGCTTGGT+GGG + chr1_4:64407477-64407496 None:intergenic 60.0%
AGTCTCGACCACCATGGGCT+TGG + chr1_4:64407481-64407500 None:intergenic 60.0%
CTAACGGCAAAACGGCCGAG+TGG + chr1_4:64407519-64407538 None:intergenic 60.0%
GCCCCTCCGACTACCACTTC+AGG - chr1_4:64406990-64407009 Msa0177450:intron 65.0%
GCTCTCACAGAGGCCCTTCC+TGG - chr1_4:64407150-64407169 Msa0177450:intron 65.0%
CTCGACCACCATGGGCTTGG+TGG + chr1_4:64407478-64407497 None:intergenic 65.0%
ACGGCAAAACGGCCGAGTGG+AGG + chr1_4:64407516-64407535 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 64404810 64408075 64404810 ID=Msa0177450;Name=Msa0177450
chr1_4 mRNA 64404810 64408075 64404810 ID=Msa0177450-mRNA-1;Parent=Msa0177450;Name=Msa0177450-mRNA-1;_AED=0.29;_eAED=0.29;_QI=170|1|1|1|0.5|0.33|3|434|448
chr1_4 exon 64404810 64406128 64404810 ID=Msa0177450-mRNA-1:exon:20952;Parent=Msa0177450-mRNA-1
chr1_4 exon 64406226 64406719 64406226 ID=Msa0177450-mRNA-1:exon:20951;Parent=Msa0177450-mRNA-1
chr1_4 exon 64407938 64408075 64407938 ID=Msa0177450-mRNA-1:exon:20950;Parent=Msa0177450-mRNA-1
chr1_4 five_prime_UTR 64407938 64408075 64407938 ID=Msa0177450-mRNA-1:five_prime_utr;Parent=Msa0177450-mRNA-1
chr1_4 five_prime_UTR 64406688 64406719 64406688 ID=Msa0177450-mRNA-1:five_prime_utr;Parent=Msa0177450-mRNA-1
chr1_4 CDS 64406226 64406687 64406226 ID=Msa0177450-mRNA-1:cds;Parent=Msa0177450-mRNA-1
chr1_4 CDS 64405244 64406128 64405244 ID=Msa0177450-mRNA-1:cds;Parent=Msa0177450-mRNA-1
chr1_4 three_prime_UTR 64404810 64405243 64404810 ID=Msa0177450-mRNA-1:three_prime_utr;Parent=Msa0177450-mRNA-1
Gene Sequence

>Msa0177450

ATGGGTAAGGAAAAGGTTCACATCAACATTGTGGTCATTGGCCATGTCGACTCTGGGAAATCAACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTCATCGAGAGGTTTGAGAAAGAAGCAGCTGAGATGAACAAGCGTTCATTCAAGTATGCCTGGGTCCTTGACAAGCTTAAGGCTGAACGGGAAAGAGGTATCACCATTGACATTGCTCTTTGGAAGTTTGAGACAACCAAGTACTACTGCACTGTCATTGATGCCCCCGGACACAGGGATTTCATTAAGAACATGATCACCGGAACATCCCAAGCTGATTGTGCCGTTCTCATCATTGATTCCACTACTGGTGGTTTTGAAGCCGGTATTTCTAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAAGCAAATGATCTGCTGTTGCAACAAGATGGATGCTACTACACCCAAGTACTCCAAGGGTAGATATGATGAAATTGTGAAGGAAGTTTCATCCTATTTGAAGAAGGTTGGCTATAACCCAGACAAAATTCCATTTGTTCCCATCTCTGGTTTTGAGGGAGATAACATGATTGAGCGCTCCACAAACCTTGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACCAAATCAATGAGCCTAAGAGGCCTTCAGACAAGCCCCTCCGACTACCACTTCAGGATGTCTACAAGATTGGAGGAATTGGAACTGTGCCTGTGGGGCGTGTTGAAACTGGTGTCATAAAACCTGGAATGGTTGTCACTTTTGCCCCTACTGGACTGACAACTGAAGTCAAGTCTGTGGAGATGCATCATGAAGCTCTCACAGAGGCCCTTCCTGGTGACAATGTGGGATTTAATGTCAAGAATGTTGCTGTTAAGGATCTCAAGCGTGGGTATGTTGCCTCAAACTCCAAGGATGACCCAGCCAAGGAGGCTGCTAACTTTACATCTCAAGTGATCATAATGAATCACCCTGGCCAGATTGGAAATGGCTATGCCCCTGTTCTTGACTGCCACACCTCCCACATTGCTGTCAAGTTTGCTGAGCTTGTTACTAAGATTGATAGGCGTTCCGGTAAGGAAATTGAGAAGGAGCCCAAATTCTTGAAGAATGGTGATGCTGGTATTATTAAGATGATTCCCACCAAGCCCATGGTGGTCGAGACTTTCTCTGAATATCCTCCACTCGGCCGTTTTGCCGTTAGAGACATGCGTCAAACTGTAGCTGTTGGGGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGTGGAGGACTTAAGCAGACCAAGTCAGCATTGAAGAAGAAGTGA
Protein sequence

>Msa0177450

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKGRYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPTGLTTEVKSVEMHHEALTEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKEIEKEPKFLKNGDAGIIKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKAVEKKDPSGGLKQTKSALKKK*