AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1349300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349300 MtrunA17_Chr1g0200071 99.554 448 2 0 1 448 1 448 0.0 916
Msa1349300 MtrunA17_Chr1g0200091 99.554 448 2 0 1 448 1 448 0.0 916
Msa1349300 MtrunA17_Chr1g0200121 99.330 448 3 0 1 448 1 448 0.0 915
Msa1349300 MtrunA17_Chr6g0458111 96.652 448 14 1 1 448 1 447 0.0 896
Msa1349300 MtrunA17_Chr6g0458091 96.652 448 14 1 1 448 1 447 0.0 896
Msa1349300 MtrunA17_Chr4g0005781 96.205 448 16 1 1 448 1 447 0.0 890
Msa1349300 MtrunA17_Chr1g0150331 91.353 451 10 2 1 448 1 425 0.0 825
Msa1349300 MtrunA17_Chr1g0193591 81.674 442 81 0 1 442 1 442 0.0 746
Msa1349300 MtrunA17_Chr1g0204031 82.151 437 73 3 1 437 1 432 0.0 736
Msa1349300 MtrunA17_Chr1g0156421 79.263 434 90 0 1 434 1 434 0.0 728
Msa1349300 MtrunA17_Chr8g0340771 79.733 449 89 1 1 447 1 449 0.0 723
Msa1349300 MtrunA17_Chr5g0415391 72.796 397 108 0 39 435 1 397 0.0 595
Msa1349300 MtrunA17_Chr1g0196251 69.727 403 94 2 4 405 5 380 0.0 576
Msa1349300 MtrunA17_Chr8g0336511 78.593 327 69 1 1 326 66 392 0.0 543
Msa1349300 MtrunA17_Chr2g0319341 73.952 334 85 1 102 433 1 334 0.0 516
Msa1349300 MtrunA17_Chr2g0298371 68.923 325 82 2 102 424 1 308 1.91e-161 457
Msa1349300 MtrunA17_Chr1g0210931 63.087 298 56 4 155 447 1 249 1.03e-118 347
Msa1349300 MtrunA17_Chr8g0340371 70.124 241 69 2 1 240 1 239 1.57e-118 346
Msa1349300 MtrunA17_Chr2g0318291 71.681 226 59 3 1 223 1 224 3.14e-112 329
Msa1349300 MtrunA17_Chr6g0483121 37.963 432 254 6 5 428 89 514 4.78e-97 301
Msa1349300 MtrunA17_Chr8g0392641 37.963 432 258 6 4 429 143 570 1.96e-92 290
Msa1349300 MtrunA17_Chr8g0380091 36.028 433 268 6 7 433 275 704 2.08e-91 291
Msa1349300 MtrunA17_Chr1g0204041 86.452 155 21 0 1 155 1 155 1.03e-90 272
Msa1349300 MtrunA17_Chr1g0200111 75.706 177 33 1 264 430 1 177 5.68e-87 263
Msa1349300 MtrunA17_Chr2g0318281 66.010 203 62 1 243 445 5 200 2.07e-84 258
Msa1349300 MtrunA17_Chr1g0156411 81.429 140 26 0 6 145 10 149 1.13e-78 241
Msa1349300 MtrunA17_Chr8g0340361 60.541 185 57 2 264 433 1 184 3.75e-66 210
Msa1349300 MtrunA17_Chr4g0026961 54.639 194 72 4 195 385 32 212 1.72e-58 191
Msa1349300 MtrunA17_Chr4g0026961 76.190 42 10 0 1 42 1 42 6.46e-13 67.8
Msa1349300 MtrunA17_Chr2g0298381 77.778 99 22 0 1 99 1 99 5.87e-48 159
Msa1349300 MtrunA17_Chr8g0341021 76.471 102 24 0 3 104 4 105 1.28e-47 159
Msa1349300 MtrunA17_Chr7g0259481 30.365 438 245 14 3 429 59 447 1.23e-45 164
Msa1349300 MtrunA17_Chr3g0116821 29.955 444 250 15 3 429 88 487 1.24e-40 151
Msa1349300 MtrunA17_Chr1g0211481 79.747 79 16 0 5 83 4 82 3.58e-36 129
Msa1349300 MtrunA17_Chr2g0319351 82.192 73 13 0 5 77 5 77 4.40e-35 125
Msa1349300 MtrunA17_Chr1g0200101 89.231 65 7 0 172 236 49 113 7.50e-35 125
Msa1349300 MtrunA17_Chr1g0200101 73.469 49 13 0 21 69 2 50 9.15e-17 76.3
Msa1349300 MtrunA17_Chr2g0287231 32.389 247 135 6 1 244 10 227 8.62e-31 118
Msa1349300 MtrunA17_Chr8g0341041 67.089 79 26 0 213 291 2 80 8.29e-28 108
Msa1349300 MtrunA17_Chr8g0341041 63.793 58 21 0 375 432 101 158 1.44e-17 80.1
Msa1349300 MtrunA17_Chr7g0261801 69.697 66 17 1 155 220 1 63 4.02e-24 95.9
Msa1349300 MtrunA17_Chr3g0130991 26.471 272 143 9 9 272 85 307 1.05e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1349300 AT1G07920.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT1G07940.4 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT1G07940.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT1G07940.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT1G07940.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT5G60390.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT5G60390.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT5G60390.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT1G07930.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1349300 AT1G07930.2 98.824 255 3 0 1 255 1 255 0.0 530
Msa1349300 AT1G07930.2 88.034 117 13 1 331 447 254 369 1.13e-65 215
Msa1349300 AT1G18070.1 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa1349300 AT1G18070.2 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa1349300 AT1G18070.3 36.927 436 256 6 5 424 99 531 4.09e-91 286
Msa1349300 AT5G10630.1 35.765 425 265 5 7 426 239 660 2.27e-90 288
Msa1349300 AT5G10630.5 35.765 425 265 5 7 426 240 661 2.32e-90 288
Msa1349300 AT5G10630.2 35.765 425 265 5 7 426 240 661 2.32e-90 288
Msa1349300 AT5G10630.3 35.765 425 265 5 7 426 310 731 1.32e-89 288
Msa1349300 AT5G10630.4 35.765 425 265 5 7 426 315 736 1.39e-89 288
Msa1349300 AT1G35550.1 78.641 103 22 0 335 437 1 103 1.62e-53 174
Msa1349300 AT4G02930.1 30.296 439 245 15 3 429 63 452 7.41e-44 160
Msa1349300 AT4G20360.1 30.137 438 257 10 3 429 75 474 1.70e-43 159
Msa1349300 AT4G20360.2 30.137 438 257 10 3 429 75 474 1.70e-43 159
Msa1349300 AT2G31060.3 25.000 264 160 8 9 263 61 295 3.24e-12 68.9
Msa1349300 AT5G08650.1 26.667 270 145 10 9 272 88 310 2.26e-11 66.6
Msa1349300 AT5G08650.2 26.667 270 145 10 9 272 88 310 2.33e-11 66.2
Msa1349300 AT2G31060.2 24.615 260 162 7 9 263 61 291 4.67e-11 65.5

Find 187 sgRNAs with CRISPR-Local

Find 281 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACCATTCTTCAAGAATT+TGG 0.066665 tig0010690:-5728 None:intergenic
TAATTCTATCAAATTGTTTC+TGG 0.156599 tig0010690:+6646 Msa1349300:three_prime_UTR
ATCATAGTTATTACAATAAA+TGG 0.259832 tig0010690:+5974 Msa1349300:three_prime_UTR
TGCTCTACCACTGAGCTATA+AGG 0.259926 tig0010690:-4184 None:intergenic
GCTCTCCTTGCTTTCACTCT+TGG 0.289982 tig0010690:+4884 Msa1349300:CDS
CAAACTATTACAAATAAAAT+AGG 0.299324 tig0010690:+6441 Msa1349300:three_prime_UTR
GTCTACAAGATTGGAGGAAT+TGG 0.315206 tig0010690:+5313 Msa1349300:CDS
GCCTCTTAGGCTCATTGATT+TGG 0.315866 tig0010690:-5255 None:intergenic
CTTAGTAAACTCAATGAGTT+TGG 0.317766 tig0010690:+6535 Msa1349300:three_prime_UTR
TTATTACAATAAATGGTCTT+TGG 0.319176 tig0010690:+5981 Msa1349300:three_prime_UTR
TGTCAAGAATGTTGCTGTTA+AGG 0.325240 tig0010690:+5489 Msa1349300:CDS
AATTTCTTATTGCTTGAGTT+TGG 0.328852 tig0010690:-6348 None:intergenic
TGGACAATGAACAAATAAAT+AGG 0.329863 tig0010690:+6555 Msa1349300:three_prime_UTR
TCACCATTCTTCAAGAATTT+GGG 0.331757 tig0010690:-5727 None:intergenic
ATAGCCAACCTTCTTCAAAT+AGG 0.332380 tig0010690:-5119 None:intergenic
TTGCGCAGCTTTCCTCTTCA+AGG 0.350006 tig0010690:+3179 Msa1349300:five_prime_UTR
ATTCCATTTGTTCCCATCTC+TGG 0.368209 tig0010690:+5154 Msa1349300:CDS
CTTCTTCTTCAATGCTGACT+TGG 0.371330 tig0010690:-5914 None:intergenic
CTCCTTGGCTGGGTCATCCT+TGG 0.371873 tig0010690:-5542 None:intergenic
ATATCTACCCTTGGAGTACT+TGG 0.380807 tig0010690:-5071 None:intergenic
ACTAAGATTGATAGGCGTTC+TGG 0.382761 tig0010690:+5685 Msa1349300:CDS
AATAAATGGTCTTTGGTCCT+TGG 0.384333 tig0010690:+5988 Msa1349300:three_prime_UTR
GCTCTAACAGAGGCCCTTCC+TGG 0.389893 tig0010690:+5448 Msa1349300:CDS
AAGCTAGCAGCCTCCTTGGC+TGG 0.398839 tig0010690:-5553 None:intergenic
GTAGCGGTCCATCAATTTCA+TGG 0.402233 tig0010690:-6500 None:intergenic
GGATCCCAGTGGAGGACTTA+AGG 0.415493 tig0010690:+5888 Msa1349300:CDS
GCTCTACCACTGAGCTATAA+GGG 0.416492 tig0010690:-4183 None:intergenic
GCAAAAGTGACAACCATTCC+AGG 0.418006 tig0010690:-5376 None:intergenic
CTCATCATTGATTCCACTAC+TGG 0.419293 tig0010690:+4815 Msa1349300:CDS
GAGGTGTGGCAGTCAAGAAC+AGG 0.420700 tig0010690:-5631 None:intergenic
TCTTCTAAAGAGAGATAAAC+GGG 0.422760 tig0010690:-6678 None:intergenic
ATGAATCACCCTGGCCAGAT+TGG 0.425183 tig0010690:+5595 Msa1349300:CDS
CCTGTGGGGCGTGTCGAGAC+TGG 0.431830 tig0010690:+5343 Msa1349300:CDS
CACCCTGGCCAGATTGGAAA+TGG 0.432985 tig0010690:+5601 Msa1349300:CDS
GCCCCTTCGACTACCACTTC+AGG 0.433104 tig0010690:+5288 Msa1349300:CDS
GTCATCGGCCATGTTGACTC+TGG 0.440627 tig0010690:+4509 Msa1349300:CDS
AGGACTAATGGGTAAGGAAA+AGG 0.440646 tig0010690:+4469 Msa1349300:exon
ACATTCTCATCCTTGCGGCT+AGG 0.443267 tig0010690:+3152 Msa1349300:five_prime_UTR
TTCTTCTAAAGAGAGATAAA+CGG 0.443312 tig0010690:-6679 None:intergenic
CATTCTCATCCTTGCGGCTA+GGG 0.444010 tig0010690:+3153 Msa1349300:five_prime_UTR
AGCGTTCATTCAAGTATGCC+TGG 0.444655 tig0010690:+4627 Msa1349300:CDS
GGGCATAGCCATTTCCAATC+TGG 0.448022 tig0010690:-5609 None:intergenic
AAACCAGAGATGGGAACAAA+TGG 0.449170 tig0010690:-5157 None:intergenic
AAAATAGGTAACATAGATTC+CGG 0.454200 tig0010690:+6456 Msa1349300:three_prime_UTR
ACTTCAGTTGTCAGTCCAGT+AGG 0.456008 tig0010690:-5400 None:intergenic
CTTCAGGATGTCTACAAGAT+TGG 0.458119 tig0010690:+5304 Msa1349300:CDS
GATTCCAACCATGAAATTGA+TGG 0.461383 tig0010690:+6492 Msa1349300:three_prime_UTR
AGTCTCAACCACCATGGGCT+TGG 0.462350 tig0010690:-5776 None:intergenic
ACCAAATCAATGAGCCTAAG+AGG 0.463105 tig0010690:+5254 Msa1349300:CDS
TGGGTCATCCTTGGAGTTTG+AGG 0.465051 tig0010690:-5533 None:intergenic
CTCAACCACCATGGGCTTGG+TGG 0.468734 tig0010690:-5773 None:intergenic
TAGGCTCATTGATTTGGTCA+AGG 0.472707 tig0010690:-5249 None:intergenic
GGCTTGTCTGAAGGCCTCTT+AGG 0.473139 tig0010690:-5268 None:intergenic
TAGCCATTTCCAATCTGGCC+AGG 0.474048 tig0010690:-5604 None:intergenic
AGGCTCATTGATTTGGTCAA+GGG 0.475242 tig0010690:-5248 None:intergenic
TGCACTGTCATTGATGCCCC+TGG 0.477178 tig0010690:+4734 Msa1349300:CDS
TTAATGAAATCCCTGTGTCC+AGG 0.479234 tig0010690:-4752 None:intergenic
AGTAAAGCTAGCAGCCTCCT+TGG 0.479718 tig0010690:-5557 None:intergenic
AGTCAGCTTTGTTAGATGGC+AGG 0.481378 tig0010690:-6374 None:intergenic
TCACCATTGACATTGCTCTC+TGG 0.481623 tig0010690:+4687 Msa1349300:CDS
AGCAGGATGATCTATCTAGT+TGG 0.481793 tig0010690:+5944 Msa1349300:three_prime_UTR
AGCTTGTTACTAAGATTGAT+AGG 0.481965 tig0010690:+5677 Msa1349300:CDS
ACAGTCAGCTTGGGATGTTC+CGG 0.483896 tig0010690:-4786 None:intergenic
AGCTAGCAGCCTCCTTGGCT+GGG 0.486002 tig0010690:-5552 None:intergenic
GCAGGATGATCTATCTAGTT+GGG 0.489479 tig0010690:+5945 Msa1349300:three_prime_UTR
TACAAACCTTGACTGGTACA+AGG 0.490199 tig0010690:+5207 Msa1349300:CDS
TCATCCTATTTGAAGAAGGT+TGG 0.491235 tig0010690:+5115 Msa1349300:CDS
GATTGATAGGCGTTCTGGTA+AGG 0.495592 tig0010690:+5690 Msa1349300:CDS
ATCTCCCTCAAAACCAGAGA+TGG 0.496573 tig0010690:-5167 None:intergenic
TGAGAACGGCACAGTCAGCT+TGG 0.498317 tig0010690:-4796 None:intergenic
CTGACCATCCTTAGAAATAC+CGG 0.501461 tig0010690:-4849 None:intergenic
AGTTTCATCCTATTTGAAGA+AGG 0.501588 tig0010690:+5111 Msa1349300:CDS
ATCATTGATTCCACTACTGG+TGG 0.502099 tig0010690:+4818 Msa1349300:CDS
GAGAACGGCACAGTCAGCTT+GGG 0.505334 tig0010690:-4795 None:intergenic
GTCAGCTTTGTTAGATGGCA+GGG 0.505354 tig0010690:-6373 None:intergenic
CTTCAGTTGTCAGTCCAGTA+GGG 0.506099 tig0010690:-5399 None:intergenic
AATTTCATCATATCTACCCT+TGG 0.508007 tig0010690:-5080 None:intergenic
AATGACAGTGCAGTAGTACT+TGG 0.509426 tig0010690:-4723 None:intergenic
CATCAAGGCTGTGGAGAAGA+AGG 0.510945 tig0010690:+5867 Msa1349300:CDS
AGGAATTGGAACTGTGCCTG+TGG 0.517140 tig0010690:+5327 Msa1349300:CDS
GGTCACCTTAAGTCCTCCAC+TGG 0.520286 tig0010690:-5893 None:intergenic
GAAGAAGAAGTGAACCGAGC+AGG 0.523086 tig0010690:+5927 Msa1349300:exon
CAGGATGTCTACAAGATTGG+AGG 0.523217 tig0010690:+5307 Msa1349300:CDS
TAATGAAATCCCTGTGTCCA+GGG 0.528272 tig0010690:-4751 None:intergenic
GAGCCCAAATTCTTGAAGAA+TGG 0.528480 tig0010690:+5724 Msa1349300:CDS
TCCCACATTGTCACCAGGAA+GGG 0.528721 tig0010690:-5461 None:intergenic
CGGTCCATCAATTTCATGGT+TGG 0.529458 tig0010690:-6496 None:intergenic
CATTGATGCCCCTGGACACA+GGG 0.535845 tig0010690:+4742 Msa1349300:CDS
GGAATTGGAACTGTGCCTGT+GGG 0.536194 tig0010690:+5328 Msa1349300:CDS
TCATTGATGCCCCTGGACAC+AGG 0.539611 tig0010690:+4741 Msa1349300:CDS
ATAAATAGGAAATACGATCC+TGG 0.539894 tig0010690:+6569 Msa1349300:three_prime_UTR
CTTCCAGAGAGCAATGTCAA+TGG 0.541189 tig0010690:-4690 None:intergenic
CATATCAACATTGTGGTCAT+CGG 0.541947 tig0010690:+4494 Msa1349300:CDS
AGTCGAAGGGGCTTGTCTGA+AGG 0.542120 tig0010690:-5277 None:intergenic
CACTTGATCTACAAGCTTGG+AGG 0.545798 tig0010690:+4551 Msa1349300:CDS
GGCTTCAAAACCACCAGTAG+TGG 0.551737 tig0010690:-4828 None:intergenic
TGTGGCTGTTGGCGTCATCA+AGG 0.552638 tig0010690:+5852 Msa1349300:CDS
AAGAGAGAATTACCTTGAAG+AGG 0.552753 tig0010690:-3191 None:intergenic
GGTCACTTGATCTACAAGCT+TGG 0.555547 tig0010690:+4548 Msa1349300:CDS
CCAGTCTCGACACGCCCCAC+AGG 0.557031 tig0010690:-5343 None:intergenic
TCAAGGGCCTCAAGAAGGGT+TGG 0.557590 tig0010690:-5232 None:intergenic
TCAACCACCATGGGCTTGGT+GGG 0.557624 tig0010690:-5772 None:intergenic
TTGGTCAAGGGCCTCAAGAA+GGG 0.558003 tig0010690:-5236 None:intergenic
TTGGACCCTTGTACCAGTCA+AGG 0.558366 tig0010690:-5213 None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG 0.559179 tig0010690:-4874 None:intergenic
CTCAAGGCTGAACGTGAAAG+AGG 0.559327 tig0010690:+4662 Msa1349300:CDS
TTTGGTCAAGGGCCTCAAGA+AGG 0.560467 tig0010690:-5237 None:intergenic
TTCTTGAAGAATGGTGATGC+TGG 0.561159 tig0010690:+5733 Msa1349300:CDS
GGAATCTCCACTCTATTGAC+CGG 0.562074 tig0010690:-6475 None:intergenic
TCTGGGAAATCAACCACCAC+TGG 0.562550 tig0010690:+4527 Msa1349300:CDS
GAAGCCGGTATTTCTAAGGA+TGG 0.564413 tig0010690:+4845 Msa1349300:CDS
GTAGCATATCCGCAAGACCT+TGG 0.566509 tig0010690:+6411 Msa1349300:three_prime_UTR
ACAAACCTTGACTGGTACAA+GGG 0.567625 tig0010690:+5208 Msa1349300:CDS
TCATCGGCCATGTTGACTCT+GGG 0.571507 tig0010690:+4510 Msa1349300:CDS
TTCATTAAGAACATGATCAC+CGG 0.572337 tig0010690:+4767 Msa1349300:CDS
AGCAAGGAGAGCATGTTCAC+GGG 0.572470 tig0010690:-4873 None:intergenic
AGCGCTCTACAAACCTTGAC+TGG 0.575793 tig0010690:+5200 Msa1349300:CDS
GAGAAAGTCTCAACCACCAT+GGG 0.575954 tig0010690:-5781 None:intergenic
ATCCTGAAGTGGTAGTCGAA+GGG 0.576252 tig0010690:-5290 None:intergenic
CTTGAGCTTGTCAAGGACCC+AGG 0.577041 tig0010690:-4645 None:intergenic
TGAATTGAACCAAGGTCTTG+CGG 0.577239 tig0010690:-6420 None:intergenic
TATCTACCCTTGGAGTACTT+GGG 0.580849 tig0010690:-5070 None:intergenic
GCATCAGTCTTGTAAACTGA+AGG 0.582139 tig0010690:+4204 Msa1349300:five_prime_UTR
TTGACAAGCGTGTCATTGAG+AGG 0.584549 tig0010690:+4576 Msa1349300:CDS
ATCCCACATTGTCACCAGGA+AGG 0.585536 tig0010690:-5462 None:intergenic
GAGACTGGTGTCATAAAACC+TGG 0.585699 tig0010690:+5358 Msa1349300:CDS
CTGGGTCCTTGACAAGCTCA+AGG 0.585742 tig0010690:+4646 Msa1349300:CDS
GGCCCTTCCTGGTGACAATG+TGG 0.586985 tig0010690:+5459 Msa1349300:CDS
GAGAATGTGAGAGTGGAGAG+TGG 0.588313 tig0010690:-3137 None:intergenic
CTGTTAAGGATCTCAAGCGT+GGG 0.589274 tig0010690:+5503 Msa1349300:CDS
CATCCTGAAGTGGTAGTCGA+AGG 0.591035 tig0010690:-5291 None:intergenic
TTCTCTGAATATCCTCCACT+CGG 0.591172 tig0010690:+5799 Msa1349300:CDS
GCCCTTCCTGGTGACAATGT+GGG 0.592402 tig0010690:+5460 Msa1349300:CDS
TTGATTTCCCAGAGTCAACA+TGG 0.596206 tig0010690:-4517 None:intergenic
TAGATATGATGAAATTGTGA+AGG 0.599770 tig0010690:+5087 Msa1349300:CDS
GCATGTCTCTAACAGCAAAA+CGG 0.600985 tig0010690:-5822 None:intergenic
CTCCAAGGATGACCCAGCCA+AGG 0.602045 tig0010690:+5540 Msa1349300:CDS
GCGTTCATTCAAGTATGCCT+GGG 0.605879 tig0010690:+4628 Msa1349300:CDS
GTCACCTTAAGTCCTCCACT+GGG 0.606003 tig0010690:-5892 None:intergenic
TCTCCCTCAAAACCAGAGAT+GGG 0.607478 tig0010690:-5166 None:intergenic
ATAGATTCCGGTCAATAGAG+TGG 0.610318 tig0010690:+6468 Msa1349300:three_prime_UTR
GATGATTCCCACCAAGCCCA+TGG 0.611182 tig0010690:+5765 Msa1349300:CDS
TACTACACCCAAGTACTCCA+AGG 0.613862 tig0010690:+5063 Msa1349300:CDS
CTCTCACATTCTCATCCTTG+CGG 0.614676 tig0010690:+3147 Msa1349300:five_prime_UTR
AAAGGTTCATATCAACATTG+TGG 0.619488 tig0010690:+4487 Msa1349300:CDS
CAAGGGTCCAACCCTTCTTG+AGG 0.620566 tig0010690:+5225 Msa1349300:CDS
TCACACCAAGAGTGAAAGCA+AGG 0.621193 tig0010690:-4889 None:intergenic
ATCTTGTAGACATCCTGAAG+TGG 0.621388 tig0010690:-5301 None:intergenic
GCTGTTAAGGATCTCAAGCG+TGG 0.622485 tig0010690:+5502 Msa1349300:CDS
TGTTGGCGTCATCAAGGCTG+TGG 0.622557 tig0010690:+5858 Msa1349300:CDS
TTCAGTTGTCAGTCCAGTAG+GGG 0.622733 tig0010690:-5398 None:intergenic
AGCCATTTCCAATCTGGCCA+GGG 0.623271 tig0010690:-5603 None:intergenic
GACAACTGAAGTCAAGTCTG+TGG 0.624055 tig0010690:+5411 Msa1349300:CDS
CAAGGATGAGAATGTGAGAG+TGG 0.624166 tig0010690:-3144 None:intergenic
ACAGCAAAACGGCCGAGTGG+AGG 0.624787 tig0010690:-5811 None:intergenic
TGGTGTCATAAAACCTGGAA+TGG 0.625384 tig0010690:+5363 Msa1349300:CDS
TAATAGTTTGAATTGAACCA+AGG 0.627102 tig0010690:-6428 None:intergenic
TCCTGAAGTGGTAGTCGAAG+GGG 0.627928 tig0010690:-5289 None:intergenic
AGGTGTGGCAGTCAAGAACA+GGG 0.628146 tig0010690:-5630 None:intergenic
GTTCAGCCTTGAGCTTGTCA+AGG 0.628494 tig0010690:-4652 None:intergenic
GACGAGAAATAGAAATACCA+AGG 0.629840 tig0010690:-6005 None:intergenic
GCATCATGAAGCTCTAACAG+AGG 0.633418 tig0010690:+5438 Msa1349300:CDS
GTATGTTGCCTCAAACTCCA+AGG 0.633897 tig0010690:+5525 Msa1349300:CDS
AGAGAAAGTCTCAACCACCA+TGG 0.641605 tig0010690:-5782 None:intergenic
ATGCGTCAAACTGTGGCTGT+TGG 0.643159 tig0010690:+5841 Msa1349300:CDS
ACTAGATAGATCATCCTGCT+CGG 0.643222 tig0010690:-5941 None:intergenic
TTCTGGTAAGGAGATTGAGA+AGG 0.643722 tig0010690:+5702 Msa1349300:CDS
GAATTGGAACTGTGCCTGTG+GGG 0.644316 tig0010690:+5329 Msa1349300:CDS
AGTGGAATCAATGATGAGAA+CGG 0.644693 tig0010690:-4810 None:intergenic
TAAGAGACAAATAAATGTAG+CGG 0.648524 tig0010690:-6516 None:intergenic
AAACTTGACAGCAATGTGAG+AGG 0.653594 tig0010690:-5650 None:intergenic
AATGAAATCCCTGTGTCCAG+GGG 0.656838 tig0010690:-4750 None:intergenic
TTAAATCCCACATTGTCACC+AGG 0.657091 tig0010690:-5466 None:intergenic
AATGATCTGCTGTTGCAACA+AGG 0.659392 tig0010690:+4916 Msa1349300:CDS
CAAGGATGACCCAGCCAAGG+AGG 0.659665 tig0010690:+5543 Msa1349300:CDS
GAGAAGAAGGATCCCAGTGG+AGG 0.667988 tig0010690:+5880 Msa1349300:CDS
ACTACACCCAAGTACTCCAA+GGG 0.678033 tig0010690:+5064 Msa1349300:CDS
CTTGTAGATCAAGTGACCAG+TGG 0.682705 tig0010690:-4543 None:intergenic
TAGAGACATGCGTCAAACTG+TGG 0.690315 tig0010690:+5834 Msa1349300:CDS
CTAACAGCAAAACGGCCGAG+TGG 0.692072 tig0010690:-5814 None:intergenic
GTAGATCAAGTGACCAGTGG+TGG 0.699002 tig0010690:-4540 None:intergenic
GTGATCATAATGAATCACCC+TGG 0.699056 tig0010690:+5586 Msa1349300:CDS
AACTTGCCCTTATAGCTCAG+TGG 0.706507 tig0010690:+4177 Msa1349300:five_prime_UTR
GATTCCCACCAAGCCCATGG+TGG 0.723849 tig0010690:+5768 Msa1349300:CDS
GGTGTGGCAGTCAAGAACAG+GGG 0.727394 tig0010690:-5629 None:intergenic
TGACAGCAATGTGAGAGGTG+TGG 0.729691 tig0010690:-5645 None:intergenic
GTGGAGAAGAAGGATCCCAG+TGG 0.734762 tig0010690:+5877 Msa1349300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGTTTTCTTTTTGATT+TGG + tig0010690:3326-3345 Msa1349300:intron 15.0%
!! ATTCATAACAATAATTAATC+AGG - tig0010690:3387-3406 None:intergenic 15.0%
!! TATTGTTATGAATTCATATA+CGG + tig0010690:3394-3413 Msa1349300:intron 15.0%
!! GATAATTATTGTTATATTGT+TGG + tig0010690:4074-4093 Msa1349300:intron 15.0%
!! TCAAATTAGTACTTAAATAA+AGG - tig0010690:4994-5013 None:intergenic 15.0%
!! ATCATAGTTATTACAATAAA+TGG + tig0010690:5974-5993 Msa1349300:three_prime_UTR 15.0%
!! CAAACTATTACAAATAAAAT+AGG + tig0010690:6441-6460 Msa1349300:three_prime_UTR 15.0%
!!! AATCGTTTATGTTTTTGAAT+CGG + tig0010690:3421-3440 Msa1349300:intron 20.0%
!!! GTTTTAATCTTTTGTTATGA+AGG + tig0010690:3446-3465 Msa1349300:intron 20.0%
!!! TTATGAAGGTGTTAATTTTT+GGG + tig0010690:3460-3479 Msa1349300:intron 20.0%
!!! TCAATAATGGAATATGTATT+GGG - tig0010690:3839-3858 None:intergenic 20.0%
!!! TTGTTTTTTTTCAGCTTTTA+AGG + tig0010690:4449-4468 Msa1349300:five_prime_UTR 20.0%
!! AATCAAATATACAACTTCAA+GGG - tig0010690:4959-4978 None:intergenic 20.0%
!! TAATCAAATATACAACTTCA+AGG - tig0010690:4960-4979 None:intergenic 20.0%
!! TTATTACAATAAATGGTCTT+TGG + tig0010690:5981-6000 Msa1349300:three_prime_UTR 20.0%
!!! TTATTTTTGTGTTTATTCCT+TGG + tig0010690:6230-6249 Msa1349300:intron 20.0%
!!! GTTTGGTTTGATTTATAATA+GGG - tig0010690:6334-6353 None:intergenic 20.0%
!!! AGTTTGGTTTGATTTATAAT+AGG - tig0010690:6335-6354 None:intergenic 20.0%
!! TAATTCTATCAAATTGTTTC+TGG + tig0010690:6646-6665 Msa1349300:three_prime_UTR 20.0%
!!! GTTATGAAGGTGTTAATTTT+TGG + tig0010690:3459-3478 Msa1349300:intron 25.0%
!!! TGTGTAAATTTGTTTGCTTT+TGG + tig0010690:3786-3805 Msa1349300:intron 25.0%
!!! GTGTAAATTTGTTTGCTTTT+GGG + tig0010690:3787-3806 Msa1349300:intron 25.0%
!! GTCAATAATGGAATATGTAT+TGG - tig0010690:3840-3859 None:intergenic 25.0%
! TACATATTCCATTATTGACA+TGG + tig0010690:3841-3860 Msa1349300:intron 25.0%
! AACTCTGACAAAAAAAAATG+AGG - tig0010690:4150-4169 None:intergenic 25.0%
!! TTCCTTTACTATTTTCTTTG+AGG + tig0010690:4393-4412 Msa1349300:intron 25.0%
! ATCAAATATACAACTTCAAG+GGG - tig0010690:4958-4977 None:intergenic 25.0%
! TAGATATGATGAAATTGTGA+AGG + tig0010690:5087-5106 Msa1349300:CDS 25.0%
! TTTATAATAGGGTAAACGAA+CGG - tig0010690:6323-6342 None:intergenic 25.0%
! AATTTCTTATTGCTTGAGTT+TGG - tig0010690:6351-6370 None:intergenic 25.0%
! TAATAGTTTGAATTGAACCA+AGG - tig0010690:6431-6450 None:intergenic 25.0%
! AAAATAGGTAACATAGATTC+CGG + tig0010690:6456-6475 Msa1349300:three_prime_UTR 25.0%
! TAAGAGACAAATAAATGTAG+CGG - tig0010690:6519-6538 None:intergenic 25.0%
! TGGACAATGAACAAATAAAT+AGG + tig0010690:6555-6574 Msa1349300:three_prime_UTR 25.0%
!!! AATTATGATTCGCTATTTTG+TGG - tig0010690:6631-6650 None:intergenic 25.0%
!! TTCTTCTAAAGAGAGATAAA+CGG - tig0010690:6682-6701 None:intergenic 25.0%
GTCAACAACACAATATAGAT+TGG - tig0010690:3594-3613 None:intergenic 30.0%
AACACACACATACACTTATT+TGG + tig0010690:3633-3652 Msa1349300:intron 30.0%
ACACACACATACACTTATTT+GGG + tig0010690:3634-3653 Msa1349300:intron 30.0%
CATGCTTCAAAAGAAAAAAG+GGG - tig0010690:3695-3714 None:intergenic 30.0%
GCATGCTTCAAAAGAAAAAA+GGG - tig0010690:3696-3715 None:intergenic 30.0%
AGCATGCTTCAAAAGAAAAA+AGG - tig0010690:3697-3716 None:intergenic 30.0%
! TGCTTGTTGAATTATTGACT+TGG + tig0010690:3738-3757 Msa1349300:intron 30.0%
TTCAATCACCATGTCAATAA+TGG - tig0010690:3852-3871 None:intergenic 30.0%
TATAAATCAGACATGCAATC+AGG - tig0010690:3947-3966 None:intergenic 30.0%
GACCTCAAAGAAAATAGTAA+AGG - tig0010690:4398-4417 None:intergenic 30.0%
! TGCTTATGCATAGTTTTCTT+TGG + tig0010690:4419-4438 Msa1349300:intron 30.0%
!! TTTCAGCTTTTAAGGACTAA+TGG + tig0010690:4457-4476 Msa1349300:exon 30.0%
!! TTCAGCTTTTAAGGACTAAT+GGG + tig0010690:4458-4477 Msa1349300:exon 30.0%
AAAGGTTCATATCAACATTG+TGG + tig0010690:4487-4506 Msa1349300:CDS 30.0%
TTCATTAAGAACATGATCAC+CGG + tig0010690:4767-4786 Msa1349300:CDS 30.0%
AATTTCATCATATCTACCCT+TGG - tig0010690:5083-5102 None:intergenic 30.0%
AGTTTCATCCTATTTGAAGA+AGG + tig0010690:5111-5130 Msa1349300:CDS 30.0%
! GAACAAATGGAATTTTGTCT+GGG - tig0010690:5147-5166 None:intergenic 30.0%
!! AGCTTGTTACTAAGATTGAT+AGG + tig0010690:5677-5696 Msa1349300:CDS 30.0%
TCACCATTCTTCAAGAATTT+GGG - tig0010690:5730-5749 None:intergenic 30.0%
ATCACCATTCTTCAAGAATT+TGG - tig0010690:5731-5750 None:intergenic 30.0%
!! AAAAAGTCAGCTTTGTTAGA+TGG - tig0010690:6381-6400 None:intergenic 30.0%
!! ATCTAACAAAGCTGACTTTT+TGG + tig0010690:6380-6399 Msa1349300:three_prime_UTR 30.0%
CTTAGTAAACTCAATGAGTT+TGG + tig0010690:6535-6554 Msa1349300:three_prime_UTR 30.0%
ATAAATAGGAAATACGATCC+TGG + tig0010690:6569-6588 Msa1349300:three_prime_UTR 30.0%
!!! TAGTTTTGATCTCATTATCC+AGG - tig0010690:6590-6609 None:intergenic 30.0%
! TCTTCTAAAGAGAGATAAAC+GGG - tig0010690:6681-6700 None:intergenic 30.0%
AAGAGAGAATTACCTTGAAG+AGG - tig0010690:3194-3213 None:intergenic 35.0%
TGCATGGGATCATTACAAAT+TGG + tig0010690:3299-3318 Msa1349300:intron 35.0%
TTACACACACATACTTGTTC+CGG + tig0010690:3989-4008 Msa1349300:intron 35.0%
ATTATCATCAGCATGCTTCA+AGG - tig0010690:4060-4079 None:intergenic 35.0%
!! CTTTTAAGGACTAATGGGTA+AGG + tig0010690:4463-4482 Msa1349300:exon 35.0%
CATATCAACATTGTGGTCAT+CGG + tig0010690:4494-4513 Msa1349300:CDS 35.0%
AGTGGAATCAATGATGAGAA+CGG - tig0010690:4813-4832 None:intergenic 35.0%
!!! TTTTGAAGCCGGTATTTCTA+AGG + tig0010690:4841-4860 Msa1349300:CDS 35.0%
ATCCATCTGTTCAAGATAGA+TGG - tig0010690:5041-5060 None:intergenic 35.0%
TCATCCTATTTGAAGAAGGT+TGG + tig0010690:5115-5134 Msa1349300:CDS 35.0%
ATAGCCAACCTTCTTCAAAT+AGG - tig0010690:5122-5141 None:intergenic 35.0%
! GGAACAAATGGAATTTTGTC+TGG - tig0010690:5148-5167 None:intergenic 35.0%
! TGTCAAGAATGTTGCTGTTA+AGG + tig0010690:5489-5508 Msa1349300:CDS 35.0%
AATAAATGGTCTTTGGTCCT+TGG + tig0010690:5988-6007 Msa1349300:three_prime_UTR 35.0%
GACGAGAAATAGAAATACCA+AGG - tig0010690:6008-6027 None:intergenic 35.0%
GAACAAAAGCTAAGAGATGA+AGG - tig0010690:6063-6082 None:intergenic 35.0%
!! TTAGCTTTTGTTCTCAGAAC+TGG + tig0010690:6070-6089 Msa1349300:intron 35.0%
! TAGCTTTTGTTCTCAGAACT+GGG + tig0010690:6071-6090 Msa1349300:intron 35.0%
! GTTGTGTCTAGTTTGAAACT+TGG + tig0010690:6146-6165 Msa1349300:intron 35.0%
! GGTACATGTCTCTATGTTTT+AGG + tig0010690:6274-6293 Msa1349300:intron 35.0%
GATTCCAACCATGAAATTGA+TGG + tig0010690:6492-6511 Msa1349300:three_prime_UTR 35.0%
AGCTTATGTACGTGATTGCA+TGG + tig0010690:3283-3302 Msa1349300:intron 40.0%
GCTTATGTACGTGATTGCAT+GGG + tig0010690:3284-3303 Msa1349300:intron 40.0%
AATAGAGTAGAGTAACGCTG+TGG + tig0010690:3667-3686 Msa1349300:intron 40.0%
! TCAATGGAGTCCTTTTAACC+CGG + tig0010690:4019-4038 Msa1349300:intron 40.0%
AGCATGCTTCAAGGAAAACA+GGG - tig0010690:4051-4070 None:intergenic 40.0%
! GCATCAGTCTTGTAAACTGA+AGG + tig0010690:4204-4223 Msa1349300:five_prime_UTR 40.0%
! CAAGTCTTATCCCACTAAGT+GGG - tig0010690:4280-4299 None:intergenic 40.0%
! AGGACTAATGGGTAAGGAAA+AGG + tig0010690:4469-4488 Msa1349300:exon 40.0%
TTGATTTCCCAGAGTCAACA+TGG - tig0010690:4520-4539 None:intergenic 40.0%
AATGACAGTGCAGTAGTACT+TGG - tig0010690:4726-4745 None:intergenic 40.0%
TAATGAAATCCCTGTGTCCA+GGG - tig0010690:4754-4773 None:intergenic 40.0%
TTAATGAAATCCCTGTGTCC+AGG - tig0010690:4755-4774 None:intergenic 40.0%
!! CTCATCATTGATTCCACTAC+TGG + tig0010690:4815-4834 Msa1349300:CDS 40.0%
!! ATCATTGATTCCACTACTGG+TGG + tig0010690:4818-4837 Msa1349300:CDS 40.0%
CTGACCATCCTTAGAAATAC+CGG - tig0010690:4852-4871 None:intergenic 40.0%
AATGATCTGCTGTTGCAACA+AGG + tig0010690:4916-4935 Msa1349300:CDS 40.0%
TGCCATCTATCTTGAACAGA+TGG + tig0010690:5036-5055 Msa1349300:intron 40.0%
! TATCTACCCTTGGAGTACTT+GGG - tig0010690:5073-5092 None:intergenic 40.0%
! ATATCTACCCTTGGAGTACT+TGG - tig0010690:5074-5093 None:intergenic 40.0%
ATTCCATTTGTTCCCATCTC+TGG + tig0010690:5154-5173 Msa1349300:CDS 40.0%
AAACCAGAGATGGGAACAAA+TGG - tig0010690:5160-5179 None:intergenic 40.0%
TACAAACCTTGACTGGTACA+AGG + tig0010690:5207-5226 Msa1349300:CDS 40.0%
ACAAACCTTGACTGGTACAA+GGG + tig0010690:5208-5227 Msa1349300:CDS 40.0%
!! AGGCTCATTGATTTGGTCAA+GGG - tig0010690:5251-5270 None:intergenic 40.0%
!! TAGGCTCATTGATTTGGTCA+AGG - tig0010690:5252-5271 None:intergenic 40.0%
ACCAAATCAATGAGCCTAAG+AGG + tig0010690:5254-5273 Msa1349300:CDS 40.0%
ATCTTGTAGACATCCTGAAG+TGG - tig0010690:5304-5323 None:intergenic 40.0%
CTTCAGGATGTCTACAAGAT+TGG + tig0010690:5304-5323 Msa1349300:CDS 40.0%
GTCTACAAGATTGGAGGAAT+TGG + tig0010690:5313-5332 Msa1349300:CDS 40.0%
!! TGGTGTCATAAAACCTGGAA+TGG + tig0010690:5363-5382 Msa1349300:CDS 40.0%
TTAAATCCCACATTGTCACC+AGG - tig0010690:5469-5488 None:intergenic 40.0%
GTGATCATAATGAATCACCC+TGG + tig0010690:5586-5605 Msa1349300:CDS 40.0%
AAACTTGACAGCAATGTGAG+AGG - tig0010690:5653-5672 None:intergenic 40.0%
ACTAAGATTGATAGGCGTTC+TGG + tig0010690:5685-5704 Msa1349300:CDS 40.0%
TTCTGGTAAGGAGATTGAGA+AGG + tig0010690:5702-5721 Msa1349300:CDS 40.0%
GAGCCCAAATTCTTGAAGAA+TGG + tig0010690:5724-5743 Msa1349300:CDS 40.0%
! TTCTTGAAGAATGGTGATGC+TGG + tig0010690:5733-5752 Msa1349300:CDS 40.0%
TTCTCTGAATATCCTCCACT+CGG + tig0010690:5799-5818 Msa1349300:CDS 40.0%
GCATGTCTCTAACAGCAAAA+CGG - tig0010690:5825-5844 None:intergenic 40.0%
! CTTCTTCTTCAATGCTGACT+TGG - tig0010690:5917-5936 None:intergenic 40.0%
ACTAGATAGATCATCCTGCT+CGG - tig0010690:5944-5963 None:intergenic 40.0%
AGCAGGATGATCTATCTAGT+TGG + tig0010690:5944-5963 Msa1349300:three_prime_UTR 40.0%
GCAGGATGATCTATCTAGTT+GGG + tig0010690:5945-5964 Msa1349300:three_prime_UTR 40.0%
CAAAAGCTAAGAGATGAAGG+TGG - tig0010690:6060-6079 None:intergenic 40.0%
TAAGCAAAGCAGTAAGTTCC+AGG + tig0010690:6175-6194 Msa1349300:intron 40.0%
AAGGCTTAAACATCACACCA+AGG - tig0010690:6250-6269 None:intergenic 40.0%
TGTGATGTTTAAGCCTTTGC+TGG + tig0010690:6253-6272 Msa1349300:intron 40.0%
ATAGAGACATGTACCAGCAA+AGG - tig0010690:6269-6288 None:intergenic 40.0%
!! CAAAGCTGACTTTTTGGCAT+AGG + tig0010690:6386-6405 Msa1349300:three_prime_UTR 40.0%
TGAATTGAACCAAGGTCTTG+CGG - tig0010690:6423-6442 None:intergenic 40.0%
ATAGATTCCGGTCAATAGAG+TGG + tig0010690:6468-6487 Msa1349300:three_prime_UTR 40.0%
!!! CGCTATTTTGTGGATTAGAG+AGG - tig0010690:6621-6640 None:intergenic 40.0%
CAAGGATGAGAATGTGAGAG+TGG - tig0010690:3147-3166 None:intergenic 45.0%
CTCTCACATTCTCATCCTTG+CGG + tig0010690:3147-3166 Msa1349300:five_prime_UTR 45.0%
! TTGTTCCGGAACTGACTCAA+TGG + tig0010690:4003-4022 Msa1349300:intron 45.0%
GCATGCTTCAAGGAAAACAG+GGG - tig0010690:4050-4069 None:intergenic 45.0%
CAGCATGCTTCAAGGAAAAC+AGG - tig0010690:4052-4071 None:intergenic 45.0%
AACTTGCCCTTATAGCTCAG+TGG + tig0010690:4177-4196 Msa1349300:five_prime_UTR 45.0%
GCTCTACCACTGAGCTATAA+GGG - tig0010690:4186-4205 None:intergenic 45.0%
TGCTCTACCACTGAGCTATA+AGG - tig0010690:4187-4206 None:intergenic 45.0%
! CCAAGTCTTATCCCACTAAG+TGG - tig0010690:4281-4300 None:intergenic 45.0%
CCACTTAGTGGGATAAGACT+TGG + tig0010690:4278-4297 Msa1349300:intron 45.0%
CTTGTAGATCAAGTGACCAG+TGG - tig0010690:4546-4565 None:intergenic 45.0%
GGTCACTTGATCTACAAGCT+TGG + tig0010690:4548-4567 Msa1349300:CDS 45.0%
CACTTGATCTACAAGCTTGG+AGG + tig0010690:4551-4570 Msa1349300:CDS 45.0%
! TTGACAAGCGTGTCATTGAG+AGG + tig0010690:4576-4595 Msa1349300:CDS 45.0%
AGCGTTCATTCAAGTATGCC+TGG + tig0010690:4627-4646 Msa1349300:CDS 45.0%
GCGTTCATTCAAGTATGCCT+GGG + tig0010690:4628-4647 Msa1349300:CDS 45.0%
TCACCATTGACATTGCTCTC+TGG + tig0010690:4687-4706 Msa1349300:CDS 45.0%
CTTCCAGAGAGCAATGTCAA+TGG - tig0010690:4693-4712 None:intergenic 45.0%
AATGAAATCCCTGTGTCCAG+GGG - tig0010690:4753-4772 None:intergenic 45.0%
!!! ACTACTGGTGGTTTTGAAGC+CGG + tig0010690:4830-4849 Msa1349300:CDS 45.0%
! GAAGCCGGTATTTCTAAGGA+TGG + tig0010690:4845-4864 Msa1349300:CDS 45.0%
! AAGCAAGGAGAGCATGTTCA+CGG - tig0010690:4877-4896 None:intergenic 45.0%
TCACACCAAGAGTGAAAGCA+AGG - tig0010690:4892-4911 None:intergenic 45.0%
TACTACACCCAAGTACTCCA+AGG + tig0010690:5063-5082 Msa1349300:CDS 45.0%
ACTACACCCAAGTACTCCAA+GGG + tig0010690:5064-5083 Msa1349300:CDS 45.0%
!!! TGTTCCCATCTCTGGTTTTG+AGG + tig0010690:5162-5181 Msa1349300:CDS 45.0%
!!! GTTCCCATCTCTGGTTTTGA+GGG + tig0010690:5163-5182 Msa1349300:CDS 45.0%
TCTCCCTCAAAACCAGAGAT+GGG - tig0010690:5169-5188 None:intergenic 45.0%
ATCTCCCTCAAAACCAGAGA+TGG - tig0010690:5170-5189 None:intergenic 45.0%
! GCCTCTTAGGCTCATTGATT+TGG - tig0010690:5258-5277 None:intergenic 45.0%
ATCCTGAAGTGGTAGTCGAA+GGG - tig0010690:5293-5312 None:intergenic 45.0%
CAGGATGTCTACAAGATTGG+AGG + tig0010690:5307-5326 Msa1349300:CDS 45.0%
!! GAGACTGGTGTCATAAAACC+TGG + tig0010690:5358-5377 Msa1349300:CDS 45.0%
GCAAAAGTGACAACCATTCC+AGG - tig0010690:5379-5398 None:intergenic 45.0%
TTCAGTTGTCAGTCCAGTAG+GGG - tig0010690:5401-5420 None:intergenic 45.0%
CTTCAGTTGTCAGTCCAGTA+GGG - tig0010690:5402-5421 None:intergenic 45.0%
ACTTCAGTTGTCAGTCCAGT+AGG - tig0010690:5403-5422 None:intergenic 45.0%
GACAACTGAAGTCAAGTCTG+TGG + tig0010690:5411-5430 Msa1349300:CDS 45.0%
! GCATCATGAAGCTCTAACAG+AGG + tig0010690:5438-5457 Msa1349300:CDS 45.0%
CTGTTAAGGATCTCAAGCGT+GGG + tig0010690:5503-5522 Msa1349300:CDS 45.0%
GTATGTTGCCTCAAACTCCA+AGG + tig0010690:5525-5544 Msa1349300:CDS 45.0%
GATTGATAGGCGTTCTGGTA+AGG + tig0010690:5690-5709 Msa1349300:CDS 45.0%
GAGAAAGTCTCAACCACCAT+GGG - tig0010690:5784-5803 None:intergenic 45.0%
AGAGAAAGTCTCAACCACCA+TGG - tig0010690:5785-5804 None:intergenic 45.0%
TAGAGACATGCGTCAAACTG+TGG + tig0010690:5834-5853 Msa1349300:CDS 45.0%
!! AGAACTGGGTTCTAGATCGA+CGG + tig0010690:6085-6104 Msa1349300:intron 45.0%
GAAACAAAGTCAGCAAGTCC+TGG - tig0010690:6196-6215 None:intergenic 45.0%
!! GTCAGCTTTGTTAGATGGCA+GGG - tig0010690:6376-6395 None:intergenic 45.0%
!! AGTCAGCTTTGTTAGATGGC+AGG - tig0010690:6377-6396 None:intergenic 45.0%
GGAATCTCCACTCTATTGAC+CGG - tig0010690:6478-6497 None:intergenic 45.0%
CGGTCCATCAATTTCATGGT+TGG - tig0010690:6499-6518 None:intergenic 45.0%
GTAGCGGTCCATCAATTTCA+TGG - tig0010690:6503-6522 None:intergenic 45.0%
GAGAATGTGAGAGTGGAGAG+TGG - tig0010690:3140-3159 None:intergenic 50.0%
ACATTCTCATCCTTGCGGCT+AGG + tig0010690:3152-3171 Msa1349300:five_prime_UTR 50.0%
CATTCTCATCCTTGCGGCTA+GGG + tig0010690:3153-3172 Msa1349300:five_prime_UTR 50.0%
TTGCGCAGCTTTCCTCTTCA+AGG + tig0010690:3179-3198 Msa1349300:five_prime_UTR 50.0%
GGACTCCATTGAGTCAGTTC+CGG - tig0010690:4011-4030 None:intergenic 50.0%
! TGGAGTCCTTTTAACCCGGT+TGG + tig0010690:4023-4042 Msa1349300:intron 50.0%
! GGAGTCCTTTTAACCCGGTT+GGG + tig0010690:4024-4043 Msa1349300:intron 50.0%
TTGTTGCCAAGCCCACTTAG+TGG + tig0010690:4266-4285 Msa1349300:intron 50.0%
TGTTGCCAAGCCCACTTAGT+GGG + tig0010690:4267-4286 Msa1349300:intron 50.0%
! CTTATCCCACTAAGTGGGCT+TGG - tig0010690:4275-4294 None:intergenic 50.0%
!! TCATCGGCCATGTTGACTCT+GGG + tig0010690:4510-4529 Msa1349300:CDS 50.0%
TCTGGGAAATCAACCACCAC+TGG + tig0010690:4527-4546 Msa1349300:CDS 50.0%
GTAGATCAAGTGACCAGTGG+TGG - tig0010690:4543-4562 None:intergenic 50.0%
GTTCAGCCTTGAGCTTGTCA+AGG - tig0010690:4655-4674 None:intergenic 50.0%
CTCAAGGCTGAACGTGAAAG+AGG + tig0010690:4662-4681 Msa1349300:CDS 50.0%
ACAGTCAGCTTGGGATGTTC+CGG - tig0010690:4789-4808 None:intergenic 50.0%
GGCTTCAAAACCACCAGTAG+TGG - tig0010690:4831-4850 None:intergenic 50.0%
! AGCAAGGAGAGCATGTTCAC+GGG - tig0010690:4876-4895 None:intergenic 50.0%
GCTCTCCTTGCTTTCACTCT+TGG + tig0010690:4884-4903 Msa1349300:CDS 50.0%
AGCGCTCTACAAACCTTGAC+TGG + tig0010690:5200-5219 Msa1349300:CDS 50.0%
TTGGACCCTTGTACCAGTCA+AGG - tig0010690:5216-5235 None:intergenic 50.0%
TTGGTCAAGGGCCTCAAGAA+GGG - tig0010690:5239-5258 None:intergenic 50.0%
TTTGGTCAAGGGCCTCAAGA+AGG - tig0010690:5240-5259 None:intergenic 50.0%
TCCTGAAGTGGTAGTCGAAG+GGG - tig0010690:5292-5311 None:intergenic 50.0%
CATCCTGAAGTGGTAGTCGA+AGG - tig0010690:5294-5313 None:intergenic 50.0%
AGGAATTGGAACTGTGCCTG+TGG + tig0010690:5327-5346 Msa1349300:CDS 50.0%
GGAATTGGAACTGTGCCTGT+GGG + tig0010690:5328-5347 Msa1349300:CDS 50.0%
GAATTGGAACTGTGCCTGTG+GGG + tig0010690:5329-5348 Msa1349300:CDS 50.0%
!!! GTTGTCACTTTTGCCCCTAC+TGG + tig0010690:5385-5404 Msa1349300:CDS 50.0%
TCCCACATTGTCACCAGGAA+GGG - tig0010690:5464-5483 None:intergenic 50.0%
ATCCCACATTGTCACCAGGA+AGG - tig0010690:5465-5484 None:intergenic 50.0%
GCTGTTAAGGATCTCAAGCG+TGG + tig0010690:5502-5521 Msa1349300:CDS 50.0%
TGGGTCATCCTTGGAGTTTG+AGG - tig0010690:5536-5555 None:intergenic 50.0%
AGTAAAGCTAGCAGCCTCCT+TGG - tig0010690:5560-5579 None:intergenic 50.0%
ATGAATCACCCTGGCCAGAT+TGG + tig0010690:5595-5614 Msa1349300:CDS 50.0%
AGCCATTTCCAATCTGGCCA+GGG - tig0010690:5606-5625 None:intergenic 50.0%
TAGCCATTTCCAATCTGGCC+AGG - tig0010690:5607-5626 None:intergenic 50.0%
GGGCATAGCCATTTCCAATC+TGG - tig0010690:5612-5631 None:intergenic 50.0%
AGGTGTGGCAGTCAAGAACA+GGG - tig0010690:5633-5652 None:intergenic 50.0%
TGACAGCAATGTGAGAGGTG+TGG - tig0010690:5648-5667 None:intergenic 50.0%
ATGCGTCAAACTGTGGCTGT+TGG + tig0010690:5841-5860 Msa1349300:CDS 50.0%
CATCAAGGCTGTGGAGAAGA+AGG + tig0010690:5867-5886 Msa1349300:CDS 50.0%
GTCACCTTAAGTCCTCCACT+GGG - tig0010690:5895-5914 None:intergenic 50.0%
GAAGAAGAAGTGAACCGAGC+AGG + tig0010690:5927-5946 Msa1349300:exon 50.0%
GTAGCATATCCGCAAGACCT+TGG + tig0010690:6411-6430 Msa1349300:three_prime_UTR 50.0%
AAGGAAAACAGGGGCCCAAC+CGG - tig0010690:4041-4060 None:intergenic 55.0%
CCTCACGCAGGATCGAACTA+CGG - tig0010690:4231-4250 None:intergenic 55.0%
CGTAGTTCGATCCTGCGTGA+GGG + tig0010690:4229-4248 Msa1349300:intron 55.0%
GAATTGAACTGCCCTCACGC+AGG - tig0010690:4243-4262 None:intergenic 55.0%
!! GTCATCGGCCATGTTGACTC+TGG + tig0010690:4509-4528 Msa1349300:CDS 55.0%
CTTGAGCTTGTCAAGGACCC+AGG - tig0010690:4648-4667 None:intergenic 55.0%
CTGGGTCCTTGACAAGCTCA+AGG + tig0010690:4646-4665 Msa1349300:CDS 55.0%
TGCACTGTCATTGATGCCCC+TGG + tig0010690:4734-4753 Msa1349300:CDS 55.0%
TCATTGATGCCCCTGGACAC+AGG + tig0010690:4741-4760 Msa1349300:CDS 55.0%
CATTGATGCCCCTGGACACA+GGG + tig0010690:4742-4761 Msa1349300:CDS 55.0%
GAGAACGGCACAGTCAGCTT+GGG - tig0010690:4798-4817 None:intergenic 55.0%
TGAGAACGGCACAGTCAGCT+TGG - tig0010690:4799-4818 None:intergenic 55.0%
CAAGGGTCCAACCCTTCTTG+AGG + tig0010690:5225-5244 Msa1349300:CDS 55.0%
TCAAGGGCCTCAAGAAGGGT+TGG - tig0010690:5235-5254 None:intergenic 55.0%
GGCTTGTCTGAAGGCCTCTT+AGG - tig0010690:5271-5290 None:intergenic 55.0%
AGTCGAAGGGGCTTGTCTGA+AGG - tig0010690:5280-5299 None:intergenic 55.0%
GCCCTTCCTGGTGACAATGT+GGG + tig0010690:5460-5479 Msa1349300:CDS 55.0%
CACCCTGGCCAGATTGGAAA+TGG + tig0010690:5601-5620 Msa1349300:CDS 55.0%
GGTGTGGCAGTCAAGAACAG+GGG - tig0010690:5632-5651 None:intergenic 55.0%
GAGGTGTGGCAGTCAAGAAC+AGG - tig0010690:5634-5653 None:intergenic 55.0%
GATGATTCCCACCAAGCCCA+TGG + tig0010690:5765-5784 Msa1349300:CDS 55.0%
! TCAACCACCATGGGCTTGGT+GGG - tig0010690:5775-5794 None:intergenic 55.0%
AGTCTCAACCACCATGGGCT+TGG - tig0010690:5779-5798 None:intergenic 55.0%
CTAACAGCAAAACGGCCGAG+TGG - tig0010690:5817-5836 None:intergenic 55.0%
TGTGGCTGTTGGCGTCATCA+AGG + tig0010690:5852-5871 Msa1349300:CDS 55.0%
TGTTGGCGTCATCAAGGCTG+TGG + tig0010690:5858-5877 Msa1349300:CDS 55.0%
GTGGAGAAGAAGGATCCCAG+TGG + tig0010690:5877-5896 Msa1349300:CDS 55.0%
GAGAAGAAGGATCCCAGTGG+AGG + tig0010690:5880-5899 Msa1349300:CDS 55.0%
GGATCCCAGTGGAGGACTTA+AGG + tig0010690:5888-5907 Msa1349300:CDS 55.0%
GGTCACCTTAAGTCCTCCAC+TGG - tig0010690:5896-5915 None:intergenic 55.0%
!! ACTGGGTTCTAGATCGACGG+TGG + tig0010690:6088-6107 Msa1349300:intron 55.0%
GCGCAAAAACCCTAGCCGCA+AGG - tig0010690:3165-3184 None:intergenic 60.0%
AGGGGCCCAACCGGGTTAAA+AGG - tig0010690:4032-4051 None:intergenic 60.0%
AGGAAAACAGGGGCCCAACC+GGG - tig0010690:4040-4059 None:intergenic 60.0%
CCGTAGTTCGATCCTGCGTG+AGG + tig0010690:4228-4247 Msa1349300:intron 60.0%
GCCCCTTCGACTACCACTTC+AGG + tig0010690:5288-5307 Msa1349300:CDS 60.0%
GCTCTAACAGAGGCCCTTCC+TGG + tig0010690:5448-5467 Msa1349300:CDS 60.0%
GGCCCTTCCTGGTGACAATG+TGG + tig0010690:5459-5478 Msa1349300:CDS 60.0%
CTCCAAGGATGACCCAGCCA+AGG + tig0010690:5540-5559 Msa1349300:CDS 60.0%
CTCCTTGGCTGGGTCATCCT+TGG - tig0010690:5545-5564 None:intergenic 60.0%
CAAGGATGACCCAGCCAAGG+AGG + tig0010690:5543-5562 Msa1349300:CDS 60.0%
AGCTAGCAGCCTCCTTGGCT+GGG - tig0010690:5555-5574 None:intergenic 60.0%
AAGCTAGCAGCCTCCTTGGC+TGG - tig0010690:5556-5575 None:intergenic 60.0%
GATTCCCACCAAGCCCATGG+TGG + tig0010690:5768-5787 Msa1349300:CDS 60.0%
CTCAACCACCATGGGCTTGG+TGG - tig0010690:5776-5795 None:intergenic 60.0%
ACAGCAAAACGGCCGAGTGG+AGG - tig0010690:5814-5833 None:intergenic 60.0%
CCAGTCTCGACACGCCCCAC+AGG - tig0010690:5346-5365 None:intergenic 70.0%
CCTGTGGGGCGTGTCGAGAC+TGG + tig0010690:5343-5362 Msa1349300:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0010690 gene 3131 6715 3131 ID=Msa1349300;Name=Msa1349300
tig0010690 mRNA 3131 6715 3131 ID=Msa1349300-mRNA-1;Parent=Msa1349300;Name=Msa1349300-mRNA-1;_AED=0.10;_eAED=0.10;_QI=161|1|1|1|0.25|0|5|480|448
tig0010690 exon 3131 3200 3131 ID=Msa1349300-mRNA-1:exon:6868;Parent=Msa1349300-mRNA-1
tig0010690 exon 4166 4225 4166 ID=Msa1349300-mRNA-1:exon:6869;Parent=Msa1349300-mRNA-1
tig0010690 exon 4445 4937 4445 ID=Msa1349300-mRNA-1:exon:6870;Parent=Msa1349300-mRNA-1
tig0010690 exon 5055 6045 5055 ID=Msa1349300-mRNA-1:exon:6871;Parent=Msa1349300-mRNA-1
tig0010690 exon 6342 6715 6342 ID=Msa1349300-mRNA-1:exon:6872;Parent=Msa1349300-mRNA-1
tig0010690 five_prime_UTR 3131 3200 3131 ID=Msa1349300-mRNA-1:five_prime_utr;Parent=Msa1349300-mRNA-1
tig0010690 five_prime_UTR 4166 4225 4166 ID=Msa1349300-mRNA-1:five_prime_utr;Parent=Msa1349300-mRNA-1
tig0010690 five_prime_UTR 4445 4475 4445 ID=Msa1349300-mRNA-1:five_prime_utr;Parent=Msa1349300-mRNA-1
tig0010690 CDS 4476 4937 4476 ID=Msa1349300-mRNA-1:cds;Parent=Msa1349300-mRNA-1
tig0010690 CDS 5055 5939 5055 ID=Msa1349300-mRNA-1:cds;Parent=Msa1349300-mRNA-1
tig0010690 three_prime_UTR 5940 6045 5940 ID=Msa1349300-mRNA-1:three_prime_utr;Parent=Msa1349300-mRNA-1
tig0010690 three_prime_UTR 6342 6715 6342 ID=Msa1349300-mRNA-1:three_prime_utr;Parent=Msa1349300-mRNA-1
Gene Sequence

>Msa1349300

ATGGGTAAGGAAAAGGTTCATATCAACATTGTGGTCATCGGCCATGTTGACTCTGGGAAATCAACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTCATTGAGAGGTTTGAGAAAGAAGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCCTGGGTCCTTGACAAGCTCAAGGCTGAACGTGAAAGAGGTATCACCATTGACATTGCTCTCTGGAAGTTTGAGACAACCAAGTACTACTGCACTGTCATTGATGCCCCTGGACACAGGGATTTCATTAAGAACATGATCACCGGAACATCCCAAGCTGACTGTGCCGTTCTCATCATTGATTCCACTACTGGTGGTTTTGAAGCCGGTATTTCTAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAAGCAAATGATCTGCTGTTGCAACAAGATGGATGCTACTACACCCAAGTACTCCAAGGGTAGATATGATGAAATTGTGAAGGAAGTTTCATCCTATTTGAAGAAGGTTGGCTATAACCCAGACAAAATTCCATTTGTTCCCATCTCTGGTTTTGAGGGAGATAACATGATTGAGCGCTCTACAAACCTTGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACCAAATCAATGAGCCTAAGAGGCCTTCAGACAAGCCCCTTCGACTACCACTTCAGGATGTCTACAAGATTGGAGGAATTGGAACTGTGCCTGTGGGGCGTGTCGAGACTGGTGTCATAAAACCTGGAATGGTTGTCACTTTTGCCCCTACTGGACTGACAACTGAAGTCAAGTCTGTGGAGATGCATCATGAAGCTCTAACAGAGGCCCTTCCTGGTGACAATGTGGGATTTAATGTCAAGAATGTTGCTGTTAAGGATCTCAAGCGTGGGTATGTTGCCTCAAACTCCAAGGATGACCCAGCCAAGGAGGCTGCTAGCTTTACTTCTCAAGTGATCATAATGAATCACCCTGGCCAGATTGGAAATGGCTATGCCCCTGTTCTTGACTGCCACACCTCTCACATTGCTGTCAAGTTTGCTGAGCTTGTTACTAAGATTGATAGGCGTTCTGGTAAGGAGATTGAGAAGGAGCCCAAATTCTTGAAGAATGGTGATGCTGGTATTATTAAGATGATTCCCACCAAGCCCATGGTGGTTGAGACTTTCTCTGAATATCCTCCACTCGGCCGTTTTGCTGTTAGAGACATGCGTCAAACTGTGGCTGTTGGCGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGTGGAGGACTTAAGGTGACCAAGTCAGCATTGAAGAAGAAGTGA
Protein sequence

>Msa1349300

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKGRYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPTGLTTEVKSVEMHHEALTEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKEIEKEPKFLKNGDAGIIKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKAVEKKDPSGGLKVTKSALKKK*