AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1444000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1444000 MtrunA17_Chr1g0200071 99.554 448 2 0 1 448 1 448 0.0 916
Msa1444000 MtrunA17_Chr1g0200091 99.554 448 2 0 1 448 1 448 0.0 916
Msa1444000 MtrunA17_Chr1g0200121 99.330 448 3 0 1 448 1 448 0.0 915
Msa1444000 MtrunA17_Chr6g0458111 96.652 448 14 1 1 448 1 447 0.0 896
Msa1444000 MtrunA17_Chr6g0458091 96.652 448 14 1 1 448 1 447 0.0 896
Msa1444000 MtrunA17_Chr4g0005781 96.205 448 16 1 1 448 1 447 0.0 890
Msa1444000 MtrunA17_Chr1g0150331 91.353 451 10 2 1 448 1 425 0.0 825
Msa1444000 MtrunA17_Chr1g0193591 81.674 442 81 0 1 442 1 442 0.0 746
Msa1444000 MtrunA17_Chr1g0204031 82.151 437 73 3 1 437 1 432 0.0 736
Msa1444000 MtrunA17_Chr1g0156421 79.263 434 90 0 1 434 1 434 0.0 728
Msa1444000 MtrunA17_Chr8g0340771 79.733 449 89 1 1 447 1 449 0.0 723
Msa1444000 MtrunA17_Chr5g0415391 72.796 397 108 0 39 435 1 397 0.0 595
Msa1444000 MtrunA17_Chr1g0196251 69.727 403 94 2 4 405 5 380 0.0 576
Msa1444000 MtrunA17_Chr8g0336511 78.593 327 69 1 1 326 66 392 0.0 543
Msa1444000 MtrunA17_Chr2g0319341 73.952 334 85 1 102 433 1 334 0.0 516
Msa1444000 MtrunA17_Chr2g0298371 68.923 325 82 2 102 424 1 308 1.91e-161 457
Msa1444000 MtrunA17_Chr1g0210931 63.087 298 56 4 155 447 1 249 1.03e-118 347
Msa1444000 MtrunA17_Chr8g0340371 70.124 241 69 2 1 240 1 239 1.57e-118 346
Msa1444000 MtrunA17_Chr2g0318291 71.681 226 59 3 1 223 1 224 3.14e-112 329
Msa1444000 MtrunA17_Chr6g0483121 37.963 432 254 6 5 428 89 514 4.78e-97 301
Msa1444000 MtrunA17_Chr8g0392641 37.963 432 258 6 4 429 143 570 1.96e-92 290
Msa1444000 MtrunA17_Chr8g0380091 36.028 433 268 6 7 433 275 704 2.08e-91 291
Msa1444000 MtrunA17_Chr1g0204041 86.452 155 21 0 1 155 1 155 1.03e-90 272
Msa1444000 MtrunA17_Chr1g0200111 75.706 177 33 1 264 430 1 177 5.68e-87 263
Msa1444000 MtrunA17_Chr2g0318281 66.010 203 62 1 243 445 5 200 2.07e-84 258
Msa1444000 MtrunA17_Chr1g0156411 81.429 140 26 0 6 145 10 149 1.13e-78 241
Msa1444000 MtrunA17_Chr8g0340361 60.541 185 57 2 264 433 1 184 3.75e-66 210
Msa1444000 MtrunA17_Chr4g0026961 54.639 194 72 4 195 385 32 212 1.72e-58 191
Msa1444000 MtrunA17_Chr4g0026961 76.190 42 10 0 1 42 1 42 6.46e-13 67.8
Msa1444000 MtrunA17_Chr2g0298381 77.778 99 22 0 1 99 1 99 5.87e-48 159
Msa1444000 MtrunA17_Chr8g0341021 76.471 102 24 0 3 104 4 105 1.28e-47 159
Msa1444000 MtrunA17_Chr7g0259481 30.365 438 245 14 3 429 59 447 1.23e-45 164
Msa1444000 MtrunA17_Chr3g0116821 29.955 444 250 15 3 429 88 487 1.24e-40 151
Msa1444000 MtrunA17_Chr1g0211481 79.747 79 16 0 5 83 4 82 3.58e-36 129
Msa1444000 MtrunA17_Chr2g0319351 82.192 73 13 0 5 77 5 77 4.40e-35 125
Msa1444000 MtrunA17_Chr1g0200101 89.231 65 7 0 172 236 49 113 7.50e-35 125
Msa1444000 MtrunA17_Chr1g0200101 73.469 49 13 0 21 69 2 50 9.15e-17 76.3
Msa1444000 MtrunA17_Chr2g0287231 32.389 247 135 6 1 244 10 227 8.62e-31 118
Msa1444000 MtrunA17_Chr8g0341041 67.089 79 26 0 213 291 2 80 8.29e-28 108
Msa1444000 MtrunA17_Chr8g0341041 63.793 58 21 0 375 432 101 158 1.44e-17 80.1
Msa1444000 MtrunA17_Chr7g0261801 69.697 66 17 1 155 220 1 63 4.02e-24 95.9
Msa1444000 MtrunA17_Chr3g0130991 26.471 272 143 9 9 272 85 307 1.05e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1444000 AT1G07920.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT1G07940.4 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT1G07940.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT1G07940.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT1G07940.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT5G60390.3 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT5G60390.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT5G60390.2 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT1G07930.1 95.302 447 20 1 1 447 1 446 0.0 884
Msa1444000 AT1G07930.2 98.824 255 3 0 1 255 1 255 0.0 530
Msa1444000 AT1G07930.2 88.034 117 13 1 331 447 254 369 1.13e-65 215
Msa1444000 AT1G18070.1 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa1444000 AT1G18070.2 37.647 425 257 5 5 424 99 520 3.50e-94 294
Msa1444000 AT1G18070.3 36.927 436 256 6 5 424 99 531 4.09e-91 286
Msa1444000 AT5G10630.1 35.765 425 265 5 7 426 239 660 2.27e-90 288
Msa1444000 AT5G10630.5 35.765 425 265 5 7 426 240 661 2.32e-90 288
Msa1444000 AT5G10630.2 35.765 425 265 5 7 426 240 661 2.32e-90 288
Msa1444000 AT5G10630.3 35.765 425 265 5 7 426 310 731 1.32e-89 288
Msa1444000 AT5G10630.4 35.765 425 265 5 7 426 315 736 1.39e-89 288
Msa1444000 AT1G35550.1 78.641 103 22 0 335 437 1 103 1.62e-53 174
Msa1444000 AT4G02930.1 30.296 439 245 15 3 429 63 452 7.41e-44 160
Msa1444000 AT4G20360.1 30.137 438 257 10 3 429 75 474 1.70e-43 159
Msa1444000 AT4G20360.2 30.137 438 257 10 3 429 75 474 1.70e-43 159
Msa1444000 AT2G31060.3 25.000 264 160 8 9 263 61 295 3.24e-12 68.9
Msa1444000 AT5G08650.1 26.667 270 145 10 9 272 88 310 2.26e-11 66.6
Msa1444000 AT5G08650.2 26.667 270 145 10 9 272 88 310 2.33e-11 66.2
Msa1444000 AT2G31060.2 24.615 260 162 7 9 263 61 291 4.67e-11 65.5

Find 188 sgRNAs with CRISPR-Local

Find 282 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCACCATTCTTCAAGAATT+TGG 0.066665 tig0030644:-15843 None:intergenic
TAATTCTATCAAATTGTTTC+TGG 0.156599 tig0030644:+16761 Msa1444000:three_prime_UTR
ATCATAGTTATTACAATAAA+TGG 0.259832 tig0030644:+16089 Msa1444000:three_prime_UTR
TGCTCTACCACTGAGCTATA+AGG 0.259926 tig0030644:-14303 None:intergenic
GCTCTCCTTGCTTTCACTCT+TGG 0.289982 tig0030644:+14999 Msa1444000:CDS
CAAACTATTACAAATAAAAT+AGG 0.299324 tig0030644:+16556 Msa1444000:three_prime_UTR
GTCTACAAGATTGGAGGAAT+TGG 0.315206 tig0030644:+15428 Msa1444000:CDS
GCCTCTTAGGCTCATTGATT+TGG 0.315866 tig0030644:-15370 None:intergenic
CTTAGTAAACTCAATGAGTT+TGG 0.317766 tig0030644:+16650 Msa1444000:three_prime_UTR
TTATTACAATAAATGGTCTT+TGG 0.319176 tig0030644:+16096 Msa1444000:three_prime_UTR
TGTCAAGAATGTTGCTGTTA+AGG 0.325240 tig0030644:+15604 Msa1444000:CDS
AATTTCTTATTGCTTGAGTT+TGG 0.328852 tig0030644:-16463 None:intergenic
TGGACAATGAACAAATAAAT+AGG 0.329863 tig0030644:+16670 Msa1444000:three_prime_UTR
TCACCATTCTTCAAGAATTT+GGG 0.331757 tig0030644:-15842 None:intergenic
ATAGCCAACCTTCTTCAAAT+AGG 0.332380 tig0030644:-15234 None:intergenic
TTGCGCAGCTTTCCTCTTCA+AGG 0.350006 tig0030644:+13298 Msa1444000:five_prime_UTR
ATTCCATTTGTTCCCATCTC+TGG 0.368209 tig0030644:+15269 Msa1444000:CDS
CTTCTTCTTCAATGCTGACT+TGG 0.371330 tig0030644:-16029 None:intergenic
CTCCTTGGCTGGGTCATCCT+TGG 0.371873 tig0030644:-15657 None:intergenic
ATATCTACCCTTGGAGTACT+TGG 0.380807 tig0030644:-15186 None:intergenic
ACTAAGATTGATAGGCGTTC+TGG 0.382761 tig0030644:+15800 Msa1444000:CDS
AATAAATGGTCTTTGGTCCT+TGG 0.384333 tig0030644:+16103 Msa1444000:three_prime_UTR
GCTCTAACAGAGGCCCTTCC+TGG 0.389893 tig0030644:+15563 Msa1444000:CDS
AAGCTAGCAGCCTCCTTGGC+TGG 0.398839 tig0030644:-15668 None:intergenic
GTAGCGGTCCATCAATTTCA+TGG 0.402233 tig0030644:-16615 None:intergenic
GGATCCCAGTGGAGGACTTA+AGG 0.415493 tig0030644:+16003 Msa1444000:CDS
GCTCTACCACTGAGCTATAA+GGG 0.416492 tig0030644:-14302 None:intergenic
GCAAAAGTGACAACCATTCC+AGG 0.418006 tig0030644:-15491 None:intergenic
CTCATCATTGATTCCACTAC+TGG 0.419293 tig0030644:+14930 Msa1444000:CDS
GAGGTGTGGCAGTCAAGAAC+AGG 0.420700 tig0030644:-15746 None:intergenic
ATGAATCACCCTGGCCAGAT+TGG 0.425183 tig0030644:+15710 Msa1444000:CDS
CCTGTGGGGCGTGTCGAGAC+TGG 0.431830 tig0030644:+15458 Msa1444000:CDS
CACCCTGGCCAGATTGGAAA+TGG 0.432985 tig0030644:+15716 Msa1444000:CDS
GCCCCTTCGACTACCACTTC+AGG 0.433104 tig0030644:+15403 Msa1444000:CDS
GTCATCGGCCATGTTGACTC+TGG 0.440627 tig0030644:+14624 Msa1444000:CDS
AGGACTAATGGGTAAGGAAA+AGG 0.440646 tig0030644:+14584 Msa1444000:exon
ACATTCTCATCCTTGCGGCT+AGG 0.443267 tig0030644:+13271 Msa1444000:five_prime_UTR
CATTCTCATCCTTGCGGCTA+GGG 0.444010 tig0030644:+13272 Msa1444000:five_prime_UTR
AGCGTTCATTCAAGTATGCC+TGG 0.444655 tig0030644:+14742 Msa1444000:CDS
GGGCATAGCCATTTCCAATC+TGG 0.448022 tig0030644:-15724 None:intergenic
AAACCAGAGATGGGAACAAA+TGG 0.449170 tig0030644:-15272 None:intergenic
AAAATAGGTAACATAGATTC+CGG 0.454200 tig0030644:+16571 Msa1444000:three_prime_UTR
ACTTCAGTTGTCAGTCCAGT+AGG 0.456008 tig0030644:-15515 None:intergenic
CTTCAGGATGTCTACAAGAT+TGG 0.458119 tig0030644:+15419 Msa1444000:CDS
GATTCCAACCATGAAATTGA+TGG 0.461383 tig0030644:+16607 Msa1444000:three_prime_UTR
AGTCTCAACCACCATGGGCT+TGG 0.462350 tig0030644:-15891 None:intergenic
ACCAAATCAATGAGCCTAAG+AGG 0.463105 tig0030644:+15369 Msa1444000:CDS
TGGGTCATCCTTGGAGTTTG+AGG 0.465051 tig0030644:-15648 None:intergenic
CACAAACCTTGACTGGTACA+AGG 0.466653 tig0030644:+15322 Msa1444000:CDS
CTCAACCACCATGGGCTTGG+TGG 0.468734 tig0030644:-15888 None:intergenic
TAGGCTCATTGATTTGGTCA+AGG 0.472707 tig0030644:-15364 None:intergenic
GGCTTGTCTGAAGGCCTCTT+AGG 0.473139 tig0030644:-15383 None:intergenic
TAGCCATTTCCAATCTGGCC+AGG 0.474048 tig0030644:-15719 None:intergenic
AGGCTCATTGATTTGGTCAA+GGG 0.475242 tig0030644:-15363 None:intergenic
TGCACTGTCATTGATGCCCC+TGG 0.477178 tig0030644:+14849 Msa1444000:CDS
TTAATGAAATCCCTGTGTCC+AGG 0.479234 tig0030644:-14867 None:intergenic
AGTAAAGCTAGCAGCCTCCT+TGG 0.479718 tig0030644:-15672 None:intergenic
AGTCAGCTTTGTTAGATGGC+AGG 0.481378 tig0030644:-16489 None:intergenic
TCACCATTGACATTGCTCTC+TGG 0.481623 tig0030644:+14802 Msa1444000:CDS
AGCAGGATGATCTATCTAGT+TGG 0.481793 tig0030644:+16059 Msa1444000:three_prime_UTR
AGCTTGTTACTAAGATTGAT+AGG 0.481965 tig0030644:+15792 Msa1444000:CDS
ACAGTCAGCTTGGGATGTTC+CGG 0.483896 tig0030644:-14901 None:intergenic
AGCTAGCAGCCTCCTTGGCT+GGG 0.486002 tig0030644:-15667 None:intergenic
GCAGGATGATCTATCTAGTT+GGG 0.489479 tig0030644:+16060 Msa1444000:three_prime_UTR
TCATCCTATTTGAAGAAGGT+TGG 0.491235 tig0030644:+15230 Msa1444000:CDS
CTTGTACCAGTCAAGGTTTG+TGG 0.492698 tig0030644:-15321 None:intergenic
GATTGATAGGCGTTCTGGTA+AGG 0.495592 tig0030644:+15805 Msa1444000:CDS
ATCTCCCTCAAAACCAGAGA+TGG 0.496573 tig0030644:-15282 None:intergenic
TGAGAACGGCACAGTCAGCT+TGG 0.498317 tig0030644:-14911 None:intergenic
CTGACCATCCTTAGAAATAC+CGG 0.501461 tig0030644:-14964 None:intergenic
AGTTTCATCCTATTTGAAGA+AGG 0.501588 tig0030644:+15226 Msa1444000:CDS
ATCATTGATTCCACTACTGG+TGG 0.502099 tig0030644:+14933 Msa1444000:CDS
GAGAACGGCACAGTCAGCTT+GGG 0.505334 tig0030644:-14910 None:intergenic
GTCAGCTTTGTTAGATGGCA+GGG 0.505354 tig0030644:-16488 None:intergenic
CTTCAGTTGTCAGTCCAGTA+GGG 0.506099 tig0030644:-15514 None:intergenic
AATTTCATCATATCTACCCT+TGG 0.508007 tig0030644:-15195 None:intergenic
AATGACAGTGCAGTAGTACT+TGG 0.509426 tig0030644:-14838 None:intergenic
CATCAAGGCTGTGGAGAAGA+AGG 0.510945 tig0030644:+15982 Msa1444000:CDS
AGGAATTGGAACTGTGCCTG+TGG 0.517140 tig0030644:+15442 Msa1444000:CDS
GGTCACCTTAAGTCCTCCAC+TGG 0.520286 tig0030644:-16008 None:intergenic
GAAGAAGAAGTGAACCGAGC+AGG 0.523086 tig0030644:+16042 Msa1444000:exon
CAGGATGTCTACAAGATTGG+AGG 0.523217 tig0030644:+15422 Msa1444000:CDS
TCTTCTAAAGAGAGATAAGC+GGG 0.525356 tig0030644:-16793 None:intergenic
TAATGAAATCCCTGTGTCCA+GGG 0.528272 tig0030644:-14866 None:intergenic
GAGCCCAAATTCTTGAAGAA+TGG 0.528480 tig0030644:+15839 Msa1444000:CDS
TCCCACATTGTCACCAGGAA+GGG 0.528721 tig0030644:-15576 None:intergenic
CGGTCCATCAATTTCATGGT+TGG 0.529458 tig0030644:-16611 None:intergenic
CATTGATGCCCCTGGACACA+GGG 0.535845 tig0030644:+14857 Msa1444000:CDS
GGAATTGGAACTGTGCCTGT+GGG 0.536194 tig0030644:+15443 Msa1444000:CDS
TCATTGATGCCCCTGGACAC+AGG 0.539611 tig0030644:+14856 Msa1444000:CDS
ATAAATAGGAAATACGATCC+TGG 0.539894 tig0030644:+16684 Msa1444000:three_prime_UTR
CTTCCAGAGAGCAATGTCAA+TGG 0.541189 tig0030644:-14805 None:intergenic
CATATCAACATTGTGGTCAT+CGG 0.541947 tig0030644:+14609 Msa1444000:CDS
AGTCGAAGGGGCTTGTCTGA+AGG 0.542120 tig0030644:-15392 None:intergenic
CACTTGATCTACAAGCTTGG+AGG 0.545798 tig0030644:+14666 Msa1444000:CDS
GGCTTCAAAACCACCAGTAG+TGG 0.551737 tig0030644:-14943 None:intergenic
TGTGGCTGTTGGCGTCATCA+AGG 0.552638 tig0030644:+15967 Msa1444000:CDS
AAGAGAGAATTACCTTGAAG+AGG 0.552753 tig0030644:-13310 None:intergenic
GGTCACTTGATCTACAAGCT+TGG 0.555547 tig0030644:+14663 Msa1444000:CDS
CCAGTCTCGACACGCCCCAC+AGG 0.557031 tig0030644:-15458 None:intergenic
TCAAGGGCCTCAAGAAGGGT+TGG 0.557590 tig0030644:-15347 None:intergenic
TCAACCACCATGGGCTTGGT+GGG 0.557624 tig0030644:-15887 None:intergenic
TTGGTCAAGGGCCTCAAGAA+GGG 0.558003 tig0030644:-15351 None:intergenic
TTGGACCCTTGTACCAGTCA+AGG 0.558366 tig0030644:-15328 None:intergenic
AAGCAAGGAGAGCATGTTCA+CGG 0.559179 tig0030644:-14989 None:intergenic
AGCGCTCCACAAACCTTGAC+TGG 0.559246 tig0030644:+15315 Msa1444000:CDS
CTCAAGGCTGAACGTGAAAG+AGG 0.559327 tig0030644:+14777 Msa1444000:CDS
TTTGGTCAAGGGCCTCAAGA+AGG 0.560467 tig0030644:-15352 None:intergenic
TTCTTGAAGAATGGTGATGC+TGG 0.561159 tig0030644:+15848 Msa1444000:CDS
GGAATCTCCACTCTATTGAC+CGG 0.562074 tig0030644:-16590 None:intergenic
TCTGGGAAATCAACCACCAC+TGG 0.562550 tig0030644:+14642 Msa1444000:CDS
GAAGCCGGTATTTCTAAGGA+TGG 0.564413 tig0030644:+14960 Msa1444000:CDS
GTAGCATATCCGCAAGACCT+TGG 0.566509 tig0030644:+16526 Msa1444000:three_prime_UTR
ACAAACCTTGACTGGTACAA+GGG 0.567625 tig0030644:+15323 Msa1444000:CDS
TCATCGGCCATGTTGACTCT+GGG 0.571507 tig0030644:+14625 Msa1444000:CDS
TTCATTAAGAACATGATCAC+CGG 0.572337 tig0030644:+14882 Msa1444000:CDS
AGCAAGGAGAGCATGTTCAC+GGG 0.572470 tig0030644:-14988 None:intergenic
GAGAAAGTCTCAACCACCAT+GGG 0.575954 tig0030644:-15896 None:intergenic
ATCCTGAAGTGGTAGTCGAA+GGG 0.576252 tig0030644:-15405 None:intergenic
CTTGAGCTTGTCAAGGACCC+AGG 0.577041 tig0030644:-14760 None:intergenic
TGAATTGAACCAAGGTCTTG+CGG 0.577239 tig0030644:-16535 None:intergenic
TATCTACCCTTGGAGTACTT+GGG 0.580849 tig0030644:-15185 None:intergenic
GCATCAGTCTTGTAAACTGA+AGG 0.582139 tig0030644:+14323 Msa1444000:five_prime_UTR
TTGACAAGCGTGTCATTGAG+AGG 0.584549 tig0030644:+14691 Msa1444000:CDS
ATCCCACATTGTCACCAGGA+AGG 0.585536 tig0030644:-15577 None:intergenic
GAGACTGGTGTCATAAAACC+TGG 0.585699 tig0030644:+15473 Msa1444000:CDS
CTGGGTCCTTGACAAGCTCA+AGG 0.585742 tig0030644:+14761 Msa1444000:CDS
GGCCCTTCCTGGTGACAATG+TGG 0.586985 tig0030644:+15574 Msa1444000:CDS
GAGAATGTGAGAGTGGAGAG+TGG 0.588313 tig0030644:-13256 None:intergenic
CTGTTAAGGATCTCAAGCGT+GGG 0.589274 tig0030644:+15618 Msa1444000:CDS
CATCCTGAAGTGGTAGTCGA+AGG 0.591035 tig0030644:-15406 None:intergenic
TTCTCTGAATATCCTCCACT+CGG 0.591172 tig0030644:+15914 Msa1444000:CDS
GCCCTTCCTGGTGACAATGT+GGG 0.592402 tig0030644:+15575 Msa1444000:CDS
TTGATTTCCCAGAGTCAACA+TGG 0.596206 tig0030644:-14632 None:intergenic
TAGATATGATGAAATTGTGA+AGG 0.599770 tig0030644:+15202 Msa1444000:CDS
GCATGTCTCTAACAGCAAAA+CGG 0.600985 tig0030644:-15937 None:intergenic
CTCCAAGGATGACCCAGCCA+AGG 0.602045 tig0030644:+15655 Msa1444000:CDS
GCGTTCATTCAAGTATGCCT+GGG 0.605879 tig0030644:+14743 Msa1444000:CDS
GTCACCTTAAGTCCTCCACT+GGG 0.606003 tig0030644:-16007 None:intergenic
TCTCCCTCAAAACCAGAGAT+GGG 0.607478 tig0030644:-15281 None:intergenic
ATAGATTCCGGTCAATAGAG+TGG 0.610318 tig0030644:+16583 Msa1444000:three_prime_UTR
GATGATTCCCACCAAGCCCA+TGG 0.611182 tig0030644:+15880 Msa1444000:CDS
TACTACACCCAAGTACTCCA+AGG 0.613862 tig0030644:+15178 Msa1444000:CDS
CTCTCACATTCTCATCCTTG+CGG 0.614676 tig0030644:+13266 Msa1444000:five_prime_UTR
AAAGGTTCATATCAACATTG+TGG 0.619488 tig0030644:+14602 Msa1444000:CDS
CAAGGGTCCAACCCTTCTTG+AGG 0.620566 tig0030644:+15340 Msa1444000:CDS
TCACACCAAGAGTGAAAGCA+AGG 0.621193 tig0030644:-15004 None:intergenic
ATCTTGTAGACATCCTGAAG+TGG 0.621388 tig0030644:-15416 None:intergenic
GCTGTTAAGGATCTCAAGCG+TGG 0.622485 tig0030644:+15617 Msa1444000:CDS
TGTTGGCGTCATCAAGGCTG+TGG 0.622557 tig0030644:+15973 Msa1444000:CDS
TTCAGTTGTCAGTCCAGTAG+GGG 0.622733 tig0030644:-15513 None:intergenic
AGCCATTTCCAATCTGGCCA+GGG 0.623271 tig0030644:-15718 None:intergenic
GACAACTGAAGTCAAGTCTG+TGG 0.624055 tig0030644:+15526 Msa1444000:CDS
CAAGGATGAGAATGTGAGAG+TGG 0.624166 tig0030644:-13263 None:intergenic
ACAGCAAAACGGCCGAGTGG+AGG 0.624787 tig0030644:-15926 None:intergenic
TGGTGTCATAAAACCTGGAA+TGG 0.625384 tig0030644:+15478 Msa1444000:CDS
TAATAGTTTGAATTGAACCA+AGG 0.627102 tig0030644:-16543 None:intergenic
TCCTGAAGTGGTAGTCGAAG+GGG 0.627928 tig0030644:-15404 None:intergenic
AGGTGTGGCAGTCAAGAACA+GGG 0.628146 tig0030644:-15745 None:intergenic
GTTCAGCCTTGAGCTTGTCA+AGG 0.628494 tig0030644:-14767 None:intergenic
GACGAGAAATAGAAATACCA+AGG 0.629840 tig0030644:-16120 None:intergenic
GCATCATGAAGCTCTAACAG+AGG 0.633418 tig0030644:+15553 Msa1444000:CDS
GTATGTTGCCTCAAACTCCA+AGG 0.633897 tig0030644:+15640 Msa1444000:CDS
AGAGAAAGTCTCAACCACCA+TGG 0.641605 tig0030644:-15897 None:intergenic
ATGCGTCAAACTGTGGCTGT+TGG 0.643159 tig0030644:+15956 Msa1444000:CDS
ACTAGATAGATCATCCTGCT+CGG 0.643222 tig0030644:-16056 None:intergenic
TTCTGGTAAGGAGATTGAGA+AGG 0.643722 tig0030644:+15817 Msa1444000:CDS
GAATTGGAACTGTGCCTGTG+GGG 0.644316 tig0030644:+15444 Msa1444000:CDS
AGTGGAATCAATGATGAGAA+CGG 0.644693 tig0030644:-14925 None:intergenic
TAAGAGACAAATAAATGTAG+CGG 0.648524 tig0030644:-16631 None:intergenic
TTCTTCTAAAGAGAGATAAG+CGG 0.652423 tig0030644:-16794 None:intergenic
AAACTTGACAGCAATGTGAG+AGG 0.653594 tig0030644:-15765 None:intergenic
AATGAAATCCCTGTGTCCAG+GGG 0.656838 tig0030644:-14865 None:intergenic
TTAAATCCCACATTGTCACC+AGG 0.657091 tig0030644:-15581 None:intergenic
AATGATCTGCTGTTGCAACA+AGG 0.659392 tig0030644:+15031 Msa1444000:CDS
CAAGGATGACCCAGCCAAGG+AGG 0.659665 tig0030644:+15658 Msa1444000:CDS
GAGAAGAAGGATCCCAGTGG+AGG 0.667988 tig0030644:+15995 Msa1444000:CDS
ACTACACCCAAGTACTCCAA+GGG 0.678033 tig0030644:+15179 Msa1444000:CDS
CTTGTAGATCAAGTGACCAG+TGG 0.682705 tig0030644:-14658 None:intergenic
TAGAGACATGCGTCAAACTG+TGG 0.690315 tig0030644:+15949 Msa1444000:CDS
CTAACAGCAAAACGGCCGAG+TGG 0.692072 tig0030644:-15929 None:intergenic
GTAGATCAAGTGACCAGTGG+TGG 0.699002 tig0030644:-14655 None:intergenic
GTGATCATAATGAATCACCC+TGG 0.699056 tig0030644:+15701 Msa1444000:CDS
AACTTGCCCTTATAGCTCAG+TGG 0.706507 tig0030644:+14296 Msa1444000:five_prime_UTR
GATTCCCACCAAGCCCATGG+TGG 0.723849 tig0030644:+15883 Msa1444000:CDS
GGTGTGGCAGTCAAGAACAG+GGG 0.727394 tig0030644:-15744 None:intergenic
TGACAGCAATGTGAGAGGTG+TGG 0.729691 tig0030644:-15760 None:intergenic
GTGGAGAAGAAGGATCCCAG+TGG 0.734762 tig0030644:+15992 Msa1444000:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGTTTTCTTTTTGATT+TGG + tig0030644:13445-13464 Msa1444000:intron 15.0%
!! GATAATTATTGTTATATTGT+TGG + tig0030644:14193-14212 Msa1444000:intron 15.0%
!! ATCATAGTTATTACAATAAA+TGG + tig0030644:16089-16108 Msa1444000:three_prime_UTR 15.0%
!! CAAACTATTACAAATAAAAT+AGG + tig0030644:16556-16575 Msa1444000:three_prime_UTR 15.0%
!! ATTCATAACAACAATTAATC+AGG - tig0030644:13506-13525 None:intergenic 20.0%
!! TGTTGTTATGAATTCATATA+CGG + tig0030644:13513-13532 Msa1444000:intron 20.0%
!!! AATCGTTTATGTTTTTGAAT+CGG + tig0030644:13540-13559 Msa1444000:intron 20.0%
!!! GTTTTAATCTTTTGTTATGA+AGG + tig0030644:13565-13584 Msa1444000:intron 20.0%
!!! TTATGAAGGTGTTAATTTTT+GGG + tig0030644:13579-13598 Msa1444000:intron 20.0%
!!! TCAATAATGGAATATGTATT+GGG - tig0030644:13958-13977 None:intergenic 20.0%
!!! TTTGTTTTTTTCAGCTTTTA+AGG + tig0030644:14564-14583 Msa1444000:five_prime_UTR 20.0%
!! AATCAAATATACAACTTCAA+GGG - tig0030644:15074-15093 None:intergenic 20.0%
!! TAATCAAATATACAACTTCA+AGG - tig0030644:15075-15094 None:intergenic 20.0%
!! TTATTACAATAAATGGTCTT+TGG + tig0030644:16096-16115 Msa1444000:three_prime_UTR 20.0%
!!! TTATTTTTGTGTTTATTCCT+TGG + tig0030644:16345-16364 Msa1444000:intron 20.0%
!!! GTTTGGTTTGATTTATAATA+GGG - tig0030644:16449-16468 None:intergenic 20.0%
!!! AGTTTGGTTTGATTTATAAT+AGG - tig0030644:16450-16469 None:intergenic 20.0%
!! TAATTCTATCAAATTGTTTC+TGG + tig0030644:16761-16780 Msa1444000:three_prime_UTR 20.0%
!!! GTTATGAAGGTGTTAATTTT+TGG + tig0030644:13578-13597 Msa1444000:intron 25.0%
!!! TGTGTAAATTTGTTTGCTTT+TGG + tig0030644:13905-13924 Msa1444000:intron 25.0%
!!! GTGTAAATTTGTTTGCTTTT+GGG + tig0030644:13906-13925 Msa1444000:intron 25.0%
!! GTCAATAATGGAATATGTAT+TGG - tig0030644:13959-13978 None:intergenic 25.0%
! TACATATTCCATTATTGACA+TGG + tig0030644:13960-13979 Msa1444000:intron 25.0%
! AACTCTGACAAAAAAAAATG+AGG - tig0030644:14269-14288 None:intergenic 25.0%
!! TTCCTTTACTATTTTCTTTG+AGG + tig0030644:14509-14528 Msa1444000:intron 25.0%
! ATCAAATATACAACTTCAAG+GGG - tig0030644:15073-15092 None:intergenic 25.0%
! TAGATATGATGAAATTGTGA+AGG + tig0030644:15202-15221 Msa1444000:CDS 25.0%
! TTTATAATAGGGTAAACGAA+CGG - tig0030644:16438-16457 None:intergenic 25.0%
! AATTTCTTATTGCTTGAGTT+TGG - tig0030644:16466-16485 None:intergenic 25.0%
! TAATAGTTTGAATTGAACCA+AGG - tig0030644:16546-16565 None:intergenic 25.0%
! AAAATAGGTAACATAGATTC+CGG + tig0030644:16571-16590 Msa1444000:three_prime_UTR 25.0%
! TAAGAGACAAATAAATGTAG+CGG - tig0030644:16634-16653 None:intergenic 25.0%
! TGGACAATGAACAAATAAAT+AGG + tig0030644:16670-16689 Msa1444000:three_prime_UTR 25.0%
!!! AATTATGATTCGCTATTTTG+TGG - tig0030644:16746-16765 None:intergenic 25.0%
GTCAACAACACAATATAGAT+TGG - tig0030644:13713-13732 None:intergenic 30.0%
AACACACACATACACTTATT+TGG + tig0030644:13752-13771 Msa1444000:intron 30.0%
ACACACACATACACTTATTT+GGG + tig0030644:13753-13772 Msa1444000:intron 30.0%
CATGCTTCAAAAGAAAAAAG+GGG - tig0030644:13814-13833 None:intergenic 30.0%
GCATGCTTCAAAAGAAAAAA+GGG - tig0030644:13815-13834 None:intergenic 30.0%
AGCATGCTTCAAAAGAAAAA+AGG - tig0030644:13816-13835 None:intergenic 30.0%
! TGCTTGTTGAATTATTGACT+TGG + tig0030644:13857-13876 Msa1444000:intron 30.0%
TTCTATCACCATGTCAATAA+TGG - tig0030644:13971-13990 None:intergenic 30.0%
TATAAATCAGACATGCAATC+AGG - tig0030644:14066-14085 None:intergenic 30.0%
GACCTCAAAGAAAATAGTAA+AGG - tig0030644:14514-14533 None:intergenic 30.0%
! TGCTTATGCATAGTTTTCTT+TGG + tig0030644:14535-14554 Msa1444000:intron 30.0%
!! TTTCAGCTTTTAAGGACTAA+TGG + tig0030644:14572-14591 Msa1444000:exon 30.0%
!! TTCAGCTTTTAAGGACTAAT+GGG + tig0030644:14573-14592 Msa1444000:exon 30.0%
AAAGGTTCATATCAACATTG+TGG + tig0030644:14602-14621 Msa1444000:CDS 30.0%
TTCATTAAGAACATGATCAC+CGG + tig0030644:14882-14901 Msa1444000:CDS 30.0%
AATTTCATCATATCTACCCT+TGG - tig0030644:15198-15217 None:intergenic 30.0%
AGTTTCATCCTATTTGAAGA+AGG + tig0030644:15226-15245 Msa1444000:CDS 30.0%
! GAACAAATGGAATTTTGTCT+GGG - tig0030644:15262-15281 None:intergenic 30.0%
!! AGCTTGTTACTAAGATTGAT+AGG + tig0030644:15792-15811 Msa1444000:CDS 30.0%
TCACCATTCTTCAAGAATTT+GGG - tig0030644:15845-15864 None:intergenic 30.0%
ATCACCATTCTTCAAGAATT+TGG - tig0030644:15846-15865 None:intergenic 30.0%
!! AAAAAGTCAGCTTTGTTAGA+TGG - tig0030644:16496-16515 None:intergenic 30.0%
!! ATCTAACAAAGCTGACTTTT+TGG + tig0030644:16495-16514 Msa1444000:three_prime_UTR 30.0%
CTTAGTAAACTCAATGAGTT+TGG + tig0030644:16650-16669 Msa1444000:three_prime_UTR 30.0%
ATAAATAGGAAATACGATCC+TGG + tig0030644:16684-16703 Msa1444000:three_prime_UTR 30.0%
!!! TAGTTTTGATCTCATTATCC+AGG - tig0030644:16705-16724 None:intergenic 30.0%
! TTCTTCTAAAGAGAGATAAG+CGG - tig0030644:16797-16816 None:intergenic 30.0%
AAGAGAGAATTACCTTGAAG+AGG - tig0030644:13313-13332 None:intergenic 35.0%
TGCATGGGATCATTACAAAT+TGG + tig0030644:13418-13437 Msa1444000:intron 35.0%
TTACACACACATACTTGTTC+AGG + tig0030644:14108-14127 Msa1444000:intron 35.0%
ATTATCATCAGCATGCTTCA+AGG - tig0030644:14179-14198 None:intergenic 35.0%
!! CTTTTAAGGACTAATGGGTA+AGG + tig0030644:14578-14597 Msa1444000:exon 35.0%
CATATCAACATTGTGGTCAT+CGG + tig0030644:14609-14628 Msa1444000:CDS 35.0%
AGTGGAATCAATGATGAGAA+CGG - tig0030644:14928-14947 None:intergenic 35.0%
!!! TTTTGAAGCCGGTATTTCTA+AGG + tig0030644:14956-14975 Msa1444000:CDS 35.0%
ATCCATCTGTTCAAGATAGA+TGG - tig0030644:15156-15175 None:intergenic 35.0%
TCATCCTATTTGAAGAAGGT+TGG + tig0030644:15230-15249 Msa1444000:CDS 35.0%
ATAGCCAACCTTCTTCAAAT+AGG - tig0030644:15237-15256 None:intergenic 35.0%
! GGAACAAATGGAATTTTGTC+TGG - tig0030644:15263-15282 None:intergenic 35.0%
! TGTCAAGAATGTTGCTGTTA+AGG + tig0030644:15604-15623 Msa1444000:CDS 35.0%
AATAAATGGTCTTTGGTCCT+TGG + tig0030644:16103-16122 Msa1444000:three_prime_UTR 35.0%
GACGAGAAATAGAAATACCA+AGG - tig0030644:16123-16142 None:intergenic 35.0%
GAACAAAAGCTAAGAGATGA+AGG - tig0030644:16178-16197 None:intergenic 35.0%
!! TTAGCTTTTGTTCTCAGAAC+TGG + tig0030644:16185-16204 Msa1444000:intron 35.0%
! TAGCTTTTGTTCTCAGAACT+GGG + tig0030644:16186-16205 Msa1444000:intron 35.0%
! GTTGTGTCTAGTTTGAAACT+TGG + tig0030644:16261-16280 Msa1444000:intron 35.0%
! GGTACATGTCTCTATGTTTT+AGG + tig0030644:16389-16408 Msa1444000:intron 35.0%
GATTCCAACCATGAAATTGA+TGG + tig0030644:16607-16626 Msa1444000:three_prime_UTR 35.0%
! TCTTCTAAAGAGAGATAAGC+GGG - tig0030644:16796-16815 None:intergenic 35.0%
AGCTTATGTACGTGATTGCA+TGG + tig0030644:13402-13421 Msa1444000:intron 40.0%
GCTTATGTACGTGATTGCAT+GGG + tig0030644:13403-13422 Msa1444000:intron 40.0%
AATAGAGTAGAGTAACGCTG+TGG + tig0030644:13786-13805 Msa1444000:intron 40.0%
ATAGAGTAGAGTAACGCTGT+GGG + tig0030644:13787-13806 Msa1444000:intron 40.0%
! TTGTTCAGGAACTGACTCAA+TGG + tig0030644:14122-14141 Msa1444000:intron 40.0%
! TCAATGGAGTCCTTTTAACC+CGG + tig0030644:14138-14157 Msa1444000:intron 40.0%
TCAGCATGCTTCAAGGAAAA+CGG - tig0030644:14172-14191 None:intergenic 40.0%
! GCATCAGTCTTGTAAACTGA+AGG + tig0030644:14323-14342 Msa1444000:five_prime_UTR 40.0%
! CAAGTCTTATCCCACTAAGT+GGG - tig0030644:14399-14418 None:intergenic 40.0%
! AGGACTAATGGGTAAGGAAA+AGG + tig0030644:14584-14603 Msa1444000:exon 40.0%
TTGATTTCCCAGAGTCAACA+TGG - tig0030644:14635-14654 None:intergenic 40.0%
AATGACAGTGCAGTAGTACT+TGG - tig0030644:14841-14860 None:intergenic 40.0%
TAATGAAATCCCTGTGTCCA+GGG - tig0030644:14869-14888 None:intergenic 40.0%
TTAATGAAATCCCTGTGTCC+AGG - tig0030644:14870-14889 None:intergenic 40.0%
!! CTCATCATTGATTCCACTAC+TGG + tig0030644:14930-14949 Msa1444000:CDS 40.0%
!! ATCATTGATTCCACTACTGG+TGG + tig0030644:14933-14952 Msa1444000:CDS 40.0%
CTGACCATCCTTAGAAATAC+CGG - tig0030644:14967-14986 None:intergenic 40.0%
AATGATCTGCTGTTGCAACA+AGG + tig0030644:15031-15050 Msa1444000:CDS 40.0%
TGCCATCTATCTTGAACAGA+TGG + tig0030644:15151-15170 Msa1444000:intron 40.0%
! TATCTACCCTTGGAGTACTT+GGG - tig0030644:15188-15207 None:intergenic 40.0%
! ATATCTACCCTTGGAGTACT+TGG - tig0030644:15189-15208 None:intergenic 40.0%
ATTCCATTTGTTCCCATCTC+TGG + tig0030644:15269-15288 Msa1444000:CDS 40.0%
AAACCAGAGATGGGAACAAA+TGG - tig0030644:15275-15294 None:intergenic 40.0%
ACAAACCTTGACTGGTACAA+GGG + tig0030644:15323-15342 Msa1444000:CDS 40.0%
!! AGGCTCATTGATTTGGTCAA+GGG - tig0030644:15366-15385 None:intergenic 40.0%
!! TAGGCTCATTGATTTGGTCA+AGG - tig0030644:15367-15386 None:intergenic 40.0%
ACCAAATCAATGAGCCTAAG+AGG + tig0030644:15369-15388 Msa1444000:CDS 40.0%
ATCTTGTAGACATCCTGAAG+TGG - tig0030644:15419-15438 None:intergenic 40.0%
CTTCAGGATGTCTACAAGAT+TGG + tig0030644:15419-15438 Msa1444000:CDS 40.0%
GTCTACAAGATTGGAGGAAT+TGG + tig0030644:15428-15447 Msa1444000:CDS 40.0%
!! TGGTGTCATAAAACCTGGAA+TGG + tig0030644:15478-15497 Msa1444000:CDS 40.0%
TTAAATCCCACATTGTCACC+AGG - tig0030644:15584-15603 None:intergenic 40.0%
GTGATCATAATGAATCACCC+TGG + tig0030644:15701-15720 Msa1444000:CDS 40.0%
AAACTTGACAGCAATGTGAG+AGG - tig0030644:15768-15787 None:intergenic 40.0%
ACTAAGATTGATAGGCGTTC+TGG + tig0030644:15800-15819 Msa1444000:CDS 40.0%
TTCTGGTAAGGAGATTGAGA+AGG + tig0030644:15817-15836 Msa1444000:CDS 40.0%
GAGCCCAAATTCTTGAAGAA+TGG + tig0030644:15839-15858 Msa1444000:CDS 40.0%
! TTCTTGAAGAATGGTGATGC+TGG + tig0030644:15848-15867 Msa1444000:CDS 40.0%
TTCTCTGAATATCCTCCACT+CGG + tig0030644:15914-15933 Msa1444000:CDS 40.0%
GCATGTCTCTAACAGCAAAA+CGG - tig0030644:15940-15959 None:intergenic 40.0%
! CTTCTTCTTCAATGCTGACT+TGG - tig0030644:16032-16051 None:intergenic 40.0%
ACTAGATAGATCATCCTGCT+CGG - tig0030644:16059-16078 None:intergenic 40.0%
AGCAGGATGATCTATCTAGT+TGG + tig0030644:16059-16078 Msa1444000:three_prime_UTR 40.0%
GCAGGATGATCTATCTAGTT+GGG + tig0030644:16060-16079 Msa1444000:three_prime_UTR 40.0%
CAAAAGCTAAGAGATGAAGG+TGG - tig0030644:16175-16194 None:intergenic 40.0%
TAAGCAAAGCAGTAAGTTCC+AGG + tig0030644:16290-16309 Msa1444000:intron 40.0%
AAGGCTTAAACATCACACCA+AGG - tig0030644:16365-16384 None:intergenic 40.0%
TGTGATGTTTAAGCCTTTGC+TGG + tig0030644:16368-16387 Msa1444000:intron 40.0%
ATAGAGACATGTACCAGCAA+AGG - tig0030644:16384-16403 None:intergenic 40.0%
!! CAAAGCTGACTTTTTGGCAT+AGG + tig0030644:16501-16520 Msa1444000:three_prime_UTR 40.0%
TGAATTGAACCAAGGTCTTG+CGG - tig0030644:16538-16557 None:intergenic 40.0%
ATAGATTCCGGTCAATAGAG+TGG + tig0030644:16583-16602 Msa1444000:three_prime_UTR 40.0%
!!! CGCTATTTTGTGGATTAGAG+AGG - tig0030644:16736-16755 None:intergenic 40.0%
CAAGGATGAGAATGTGAGAG+TGG - tig0030644:13266-13285 None:intergenic 45.0%
CTCTCACATTCTCATCCTTG+CGG + tig0030644:13266-13285 Msa1444000:five_prime_UTR 45.0%
AGCATGCTTCAAGGAAAACG+GGG - tig0030644:14170-14189 None:intergenic 45.0%
CAGCATGCTTCAAGGAAAAC+GGG - tig0030644:14171-14190 None:intergenic 45.0%
AACTTGCCCTTATAGCTCAG+TGG + tig0030644:14296-14315 Msa1444000:five_prime_UTR 45.0%
GCTCTACCACTGAGCTATAA+GGG - tig0030644:14305-14324 None:intergenic 45.0%
TGCTCTACCACTGAGCTATA+AGG - tig0030644:14306-14325 None:intergenic 45.0%
! CCAAGTCTTATCCCACTAAG+TGG - tig0030644:14400-14419 None:intergenic 45.0%
CCACTTAGTGGGATAAGACT+TGG + tig0030644:14397-14416 Msa1444000:intron 45.0%
CTTGTAGATCAAGTGACCAG+TGG - tig0030644:14661-14680 None:intergenic 45.0%
GGTCACTTGATCTACAAGCT+TGG + tig0030644:14663-14682 Msa1444000:CDS 45.0%
CACTTGATCTACAAGCTTGG+AGG + tig0030644:14666-14685 Msa1444000:CDS 45.0%
! TTGACAAGCGTGTCATTGAG+AGG + tig0030644:14691-14710 Msa1444000:CDS 45.0%
AGCGTTCATTCAAGTATGCC+TGG + tig0030644:14742-14761 Msa1444000:CDS 45.0%
GCGTTCATTCAAGTATGCCT+GGG + tig0030644:14743-14762 Msa1444000:CDS 45.0%
TCACCATTGACATTGCTCTC+TGG + tig0030644:14802-14821 Msa1444000:CDS 45.0%
CTTCCAGAGAGCAATGTCAA+TGG - tig0030644:14808-14827 None:intergenic 45.0%
AATGAAATCCCTGTGTCCAG+GGG - tig0030644:14868-14887 None:intergenic 45.0%
!!! ACTACTGGTGGTTTTGAAGC+CGG + tig0030644:14945-14964 Msa1444000:CDS 45.0%
! GAAGCCGGTATTTCTAAGGA+TGG + tig0030644:14960-14979 Msa1444000:CDS 45.0%
! AAGCAAGGAGAGCATGTTCA+CGG - tig0030644:14992-15011 None:intergenic 45.0%
TCACACCAAGAGTGAAAGCA+AGG - tig0030644:15007-15026 None:intergenic 45.0%
TACTACACCCAAGTACTCCA+AGG + tig0030644:15178-15197 Msa1444000:CDS 45.0%
ACTACACCCAAGTACTCCAA+GGG + tig0030644:15179-15198 Msa1444000:CDS 45.0%
!!! TGTTCCCATCTCTGGTTTTG+AGG + tig0030644:15277-15296 Msa1444000:CDS 45.0%
!!! GTTCCCATCTCTGGTTTTGA+GGG + tig0030644:15278-15297 Msa1444000:CDS 45.0%
TCTCCCTCAAAACCAGAGAT+GGG - tig0030644:15284-15303 None:intergenic 45.0%
ATCTCCCTCAAAACCAGAGA+TGG - tig0030644:15285-15304 None:intergenic 45.0%
CTTGTACCAGTCAAGGTTTG+TGG - tig0030644:15324-15343 None:intergenic 45.0%
CACAAACCTTGACTGGTACA+AGG + tig0030644:15322-15341 Msa1444000:CDS 45.0%
! GCCTCTTAGGCTCATTGATT+TGG - tig0030644:15373-15392 None:intergenic 45.0%
ATCCTGAAGTGGTAGTCGAA+GGG - tig0030644:15408-15427 None:intergenic 45.0%
CAGGATGTCTACAAGATTGG+AGG + tig0030644:15422-15441 Msa1444000:CDS 45.0%
!! GAGACTGGTGTCATAAAACC+TGG + tig0030644:15473-15492 Msa1444000:CDS 45.0%
GCAAAAGTGACAACCATTCC+AGG - tig0030644:15494-15513 None:intergenic 45.0%
TTCAGTTGTCAGTCCAGTAG+GGG - tig0030644:15516-15535 None:intergenic 45.0%
CTTCAGTTGTCAGTCCAGTA+GGG - tig0030644:15517-15536 None:intergenic 45.0%
ACTTCAGTTGTCAGTCCAGT+AGG - tig0030644:15518-15537 None:intergenic 45.0%
GACAACTGAAGTCAAGTCTG+TGG + tig0030644:15526-15545 Msa1444000:CDS 45.0%
! GCATCATGAAGCTCTAACAG+AGG + tig0030644:15553-15572 Msa1444000:CDS 45.0%
CTGTTAAGGATCTCAAGCGT+GGG + tig0030644:15618-15637 Msa1444000:CDS 45.0%
GTATGTTGCCTCAAACTCCA+AGG + tig0030644:15640-15659 Msa1444000:CDS 45.0%
GATTGATAGGCGTTCTGGTA+AGG + tig0030644:15805-15824 Msa1444000:CDS 45.0%
GAGAAAGTCTCAACCACCAT+GGG - tig0030644:15899-15918 None:intergenic 45.0%
AGAGAAAGTCTCAACCACCA+TGG - tig0030644:15900-15919 None:intergenic 45.0%
TAGAGACATGCGTCAAACTG+TGG + tig0030644:15949-15968 Msa1444000:CDS 45.0%
!! AGAACTGGGTTCTAGATCGA+CGG + tig0030644:16200-16219 Msa1444000:intron 45.0%
GAAACAAAGTCAGCAAGTCC+TGG - tig0030644:16311-16330 None:intergenic 45.0%
!! GTCAGCTTTGTTAGATGGCA+GGG - tig0030644:16491-16510 None:intergenic 45.0%
!! AGTCAGCTTTGTTAGATGGC+AGG - tig0030644:16492-16511 None:intergenic 45.0%
GGAATCTCCACTCTATTGAC+CGG - tig0030644:16593-16612 None:intergenic 45.0%
CGGTCCATCAATTTCATGGT+TGG - tig0030644:16614-16633 None:intergenic 45.0%
GTAGCGGTCCATCAATTTCA+TGG - tig0030644:16618-16637 None:intergenic 45.0%
GAGAATGTGAGAGTGGAGAG+TGG - tig0030644:13259-13278 None:intergenic 50.0%
ACATTCTCATCCTTGCGGCT+AGG + tig0030644:13271-13290 Msa1444000:five_prime_UTR 50.0%
CATTCTCATCCTTGCGGCTA+GGG + tig0030644:13272-13291 Msa1444000:five_prime_UTR 50.0%
TTGCGCAGCTTTCCTCTTCA+AGG + tig0030644:13298-13317 Msa1444000:five_prime_UTR 50.0%
! TGGAGTCCTTTTAACCCGGT+TGG + tig0030644:14142-14161 Msa1444000:intron 50.0%
! GGAGTCCTTTTAACCCGGTT+GGG + tig0030644:14143-14162 Msa1444000:intron 50.0%
GCATGCTTCAAGGAAAACGG+GGG - tig0030644:14169-14188 None:intergenic 50.0%
TTGTTGCCAAGCCCACTTAG+TGG + tig0030644:14385-14404 Msa1444000:intron 50.0%
TGTTGCCAAGCCCACTTAGT+GGG + tig0030644:14386-14405 Msa1444000:intron 50.0%
! CTTATCCCACTAAGTGGGCT+TGG - tig0030644:14394-14413 None:intergenic 50.0%
!! TCATCGGCCATGTTGACTCT+GGG + tig0030644:14625-14644 Msa1444000:CDS 50.0%
TCTGGGAAATCAACCACCAC+TGG + tig0030644:14642-14661 Msa1444000:CDS 50.0%
GTAGATCAAGTGACCAGTGG+TGG - tig0030644:14658-14677 None:intergenic 50.0%
GTTCAGCCTTGAGCTTGTCA+AGG - tig0030644:14770-14789 None:intergenic 50.0%
CTCAAGGCTGAACGTGAAAG+AGG + tig0030644:14777-14796 Msa1444000:CDS 50.0%
ACAGTCAGCTTGGGATGTTC+CGG - tig0030644:14904-14923 None:intergenic 50.0%
GGCTTCAAAACCACCAGTAG+TGG - tig0030644:14946-14965 None:intergenic 50.0%
! AGCAAGGAGAGCATGTTCAC+GGG - tig0030644:14991-15010 None:intergenic 50.0%
GCTCTCCTTGCTTTCACTCT+TGG + tig0030644:14999-15018 Msa1444000:CDS 50.0%
TTGGACCCTTGTACCAGTCA+AGG - tig0030644:15331-15350 None:intergenic 50.0%
TTGGTCAAGGGCCTCAAGAA+GGG - tig0030644:15354-15373 None:intergenic 50.0%
TTTGGTCAAGGGCCTCAAGA+AGG - tig0030644:15355-15374 None:intergenic 50.0%
TCCTGAAGTGGTAGTCGAAG+GGG - tig0030644:15407-15426 None:intergenic 50.0%
CATCCTGAAGTGGTAGTCGA+AGG - tig0030644:15409-15428 None:intergenic 50.0%
AGGAATTGGAACTGTGCCTG+TGG + tig0030644:15442-15461 Msa1444000:CDS 50.0%
GGAATTGGAACTGTGCCTGT+GGG + tig0030644:15443-15462 Msa1444000:CDS 50.0%
GAATTGGAACTGTGCCTGTG+GGG + tig0030644:15444-15463 Msa1444000:CDS 50.0%
!!! GTTGTCACTTTTGCCCCTAC+TGG + tig0030644:15500-15519 Msa1444000:CDS 50.0%
TCCCACATTGTCACCAGGAA+GGG - tig0030644:15579-15598 None:intergenic 50.0%
ATCCCACATTGTCACCAGGA+AGG - tig0030644:15580-15599 None:intergenic 50.0%
GCTGTTAAGGATCTCAAGCG+TGG + tig0030644:15617-15636 Msa1444000:CDS 50.0%
TGGGTCATCCTTGGAGTTTG+AGG - tig0030644:15651-15670 None:intergenic 50.0%
AGTAAAGCTAGCAGCCTCCT+TGG - tig0030644:15675-15694 None:intergenic 50.0%
ATGAATCACCCTGGCCAGAT+TGG + tig0030644:15710-15729 Msa1444000:CDS 50.0%
AGCCATTTCCAATCTGGCCA+GGG - tig0030644:15721-15740 None:intergenic 50.0%
TAGCCATTTCCAATCTGGCC+AGG - tig0030644:15722-15741 None:intergenic 50.0%
GGGCATAGCCATTTCCAATC+TGG - tig0030644:15727-15746 None:intergenic 50.0%
AGGTGTGGCAGTCAAGAACA+GGG - tig0030644:15748-15767 None:intergenic 50.0%
TGACAGCAATGTGAGAGGTG+TGG - tig0030644:15763-15782 None:intergenic 50.0%
ATGCGTCAAACTGTGGCTGT+TGG + tig0030644:15956-15975 Msa1444000:CDS 50.0%
CATCAAGGCTGTGGAGAAGA+AGG + tig0030644:15982-16001 Msa1444000:CDS 50.0%
GTCACCTTAAGTCCTCCACT+GGG - tig0030644:16010-16029 None:intergenic 50.0%
GAAGAAGAAGTGAACCGAGC+AGG + tig0030644:16042-16061 Msa1444000:exon 50.0%
GTAGCATATCCGCAAGACCT+TGG + tig0030644:16526-16545 Msa1444000:three_prime_UTR 50.0%
CCTCACGCAGGATCGAACTA+CGG - tig0030644:14350-14369 None:intergenic 55.0%
CGTAGTTCGATCCTGCGTGA+GGG + tig0030644:14348-14367 Msa1444000:intron 55.0%
GAATTGAACTGCCCTCACGC+AGG - tig0030644:14362-14381 None:intergenic 55.0%
!! GTCATCGGCCATGTTGACTC+TGG + tig0030644:14624-14643 Msa1444000:CDS 55.0%
CTTGAGCTTGTCAAGGACCC+AGG - tig0030644:14763-14782 None:intergenic 55.0%
CTGGGTCCTTGACAAGCTCA+AGG + tig0030644:14761-14780 Msa1444000:CDS 55.0%
TGCACTGTCATTGATGCCCC+TGG + tig0030644:14849-14868 Msa1444000:CDS 55.0%
TCATTGATGCCCCTGGACAC+AGG + tig0030644:14856-14875 Msa1444000:CDS 55.0%
CATTGATGCCCCTGGACACA+GGG + tig0030644:14857-14876 Msa1444000:CDS 55.0%
GAGAACGGCACAGTCAGCTT+GGG - tig0030644:14913-14932 None:intergenic 55.0%
TGAGAACGGCACAGTCAGCT+TGG - tig0030644:14914-14933 None:intergenic 55.0%
AGCGCTCCACAAACCTTGAC+TGG + tig0030644:15315-15334 Msa1444000:CDS 55.0%
CAAGGGTCCAACCCTTCTTG+AGG + tig0030644:15340-15359 Msa1444000:CDS 55.0%
TCAAGGGCCTCAAGAAGGGT+TGG - tig0030644:15350-15369 None:intergenic 55.0%
GGCTTGTCTGAAGGCCTCTT+AGG - tig0030644:15386-15405 None:intergenic 55.0%
AGTCGAAGGGGCTTGTCTGA+AGG - tig0030644:15395-15414 None:intergenic 55.0%
GCCCTTCCTGGTGACAATGT+GGG + tig0030644:15575-15594 Msa1444000:CDS 55.0%
CACCCTGGCCAGATTGGAAA+TGG + tig0030644:15716-15735 Msa1444000:CDS 55.0%
GGTGTGGCAGTCAAGAACAG+GGG - tig0030644:15747-15766 None:intergenic 55.0%
GAGGTGTGGCAGTCAAGAAC+AGG - tig0030644:15749-15768 None:intergenic 55.0%
GATGATTCCCACCAAGCCCA+TGG + tig0030644:15880-15899 Msa1444000:CDS 55.0%
! TCAACCACCATGGGCTTGGT+GGG - tig0030644:15890-15909 None:intergenic 55.0%
AGTCTCAACCACCATGGGCT+TGG - tig0030644:15894-15913 None:intergenic 55.0%
CTAACAGCAAAACGGCCGAG+TGG - tig0030644:15932-15951 None:intergenic 55.0%
TGTGGCTGTTGGCGTCATCA+AGG + tig0030644:15967-15986 Msa1444000:CDS 55.0%
TGTTGGCGTCATCAAGGCTG+TGG + tig0030644:15973-15992 Msa1444000:CDS 55.0%
GTGGAGAAGAAGGATCCCAG+TGG + tig0030644:15992-16011 Msa1444000:CDS 55.0%
GAGAAGAAGGATCCCAGTGG+AGG + tig0030644:15995-16014 Msa1444000:CDS 55.0%
GGATCCCAGTGGAGGACTTA+AGG + tig0030644:16003-16022 Msa1444000:CDS 55.0%
GGTCACCTTAAGTCCTCCAC+TGG - tig0030644:16011-16030 None:intergenic 55.0%
!! ACTGGGTTCTAGATCGACGG+TGG + tig0030644:16203-16222 Msa1444000:intron 55.0%
GCGCAAAAACCCTAGCCGCA+AGG - tig0030644:13284-13303 None:intergenic 60.0%
AAGGAAAACGGGGGCCCAAC+CGG - tig0030644:14160-14179 None:intergenic 60.0%
CCGTAGTTCGATCCTGCGTG+AGG + tig0030644:14347-14366 Msa1444000:intron 60.0%
GCCCCTTCGACTACCACTTC+AGG + tig0030644:15403-15422 Msa1444000:CDS 60.0%
GCTCTAACAGAGGCCCTTCC+TGG + tig0030644:15563-15582 Msa1444000:CDS 60.0%
GGCCCTTCCTGGTGACAATG+TGG + tig0030644:15574-15593 Msa1444000:CDS 60.0%
CTCCAAGGATGACCCAGCCA+AGG + tig0030644:15655-15674 Msa1444000:CDS 60.0%
CTCCTTGGCTGGGTCATCCT+TGG - tig0030644:15660-15679 None:intergenic 60.0%
CAAGGATGACCCAGCCAAGG+AGG + tig0030644:15658-15677 Msa1444000:CDS 60.0%
AGCTAGCAGCCTCCTTGGCT+GGG - tig0030644:15670-15689 None:intergenic 60.0%
AAGCTAGCAGCCTCCTTGGC+TGG - tig0030644:15671-15690 None:intergenic 60.0%
GATTCCCACCAAGCCCATGG+TGG + tig0030644:15883-15902 Msa1444000:CDS 60.0%
CTCAACCACCATGGGCTTGG+TGG - tig0030644:15891-15910 None:intergenic 60.0%
ACAGCAAAACGGCCGAGTGG+AGG - tig0030644:15929-15948 None:intergenic 60.0%
GGGGGCCCAACCGGGTTAAA+AGG - tig0030644:14151-14170 None:intergenic 65.0%
AGGAAAACGGGGGCCCAACC+GGG - tig0030644:14159-14178 None:intergenic 65.0%
CCAGTCTCGACACGCCCCAC+AGG - tig0030644:15461-15480 None:intergenic 70.0%
CCTGTGGGGCGTGTCGAGAC+TGG + tig0030644:15458-15477 Msa1444000:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0030644 gene 13250 16830 13250 ID=Msa1444000;Name=Msa1444000
tig0030644 mRNA 13250 16830 13250 ID=Msa1444000-mRNA-1;Parent=Msa1444000;Name=Msa1444000-mRNA-1;_AED=0.10;_eAED=0.10;_QI=160|1|1|1|0.25|0|5|480|448
tig0030644 exon 13250 13319 13250 ID=Msa1444000-mRNA-1:exon:14242;Parent=Msa1444000-mRNA-1
tig0030644 exon 14285 14344 14285 ID=Msa1444000-mRNA-1:exon:14243;Parent=Msa1444000-mRNA-1
tig0030644 exon 14561 15052 14561 ID=Msa1444000-mRNA-1:exon:14244;Parent=Msa1444000-mRNA-1
tig0030644 exon 15170 16160 15170 ID=Msa1444000-mRNA-1:exon:14245;Parent=Msa1444000-mRNA-1
tig0030644 exon 16457 16830 16457 ID=Msa1444000-mRNA-1:exon:14246;Parent=Msa1444000-mRNA-1
tig0030644 five_prime_UTR 13250 13319 13250 ID=Msa1444000-mRNA-1:five_prime_utr;Parent=Msa1444000-mRNA-1
tig0030644 five_prime_UTR 14285 14344 14285 ID=Msa1444000-mRNA-1:five_prime_utr;Parent=Msa1444000-mRNA-1
tig0030644 five_prime_UTR 14561 14590 14561 ID=Msa1444000-mRNA-1:five_prime_utr;Parent=Msa1444000-mRNA-1
tig0030644 CDS 14591 15052 14591 ID=Msa1444000-mRNA-1:cds;Parent=Msa1444000-mRNA-1
tig0030644 CDS 15170 16054 15170 ID=Msa1444000-mRNA-1:cds;Parent=Msa1444000-mRNA-1
tig0030644 three_prime_UTR 16055 16160 16055 ID=Msa1444000-mRNA-1:three_prime_utr;Parent=Msa1444000-mRNA-1
tig0030644 three_prime_UTR 16457 16830 16457 ID=Msa1444000-mRNA-1:three_prime_utr;Parent=Msa1444000-mRNA-1
Gene Sequence

>Msa1444000

ATGGGTAAGGAAAAGGTTCATATCAACATTGTGGTCATCGGCCATGTTGACTCTGGGAAATCAACCACCACTGGTCACTTGATCTACAAGCTTGGAGGTATTGACAAGCGTGTCATTGAGAGGTTTGAGAAAGAAGCTGCTGAGATGAACAAGCGTTCATTCAAGTATGCCTGGGTCCTTGACAAGCTCAAGGCTGAACGTGAAAGAGGTATCACCATTGACATTGCTCTCTGGAAGTTTGAGACAACCAAGTACTACTGCACTGTCATTGATGCCCCTGGACACAGGGATTTCATTAAGAACATGATCACCGGAACATCCCAAGCTGACTGTGCCGTTCTCATCATTGATTCCACTACTGGTGGTTTTGAAGCCGGTATTTCTAAGGATGGTCAGACCCGTGAACATGCTCTCCTTGCTTTCACTCTTGGTGTGAAGCAAATGATCTGCTGTTGCAACAAGATGGATGCTACTACACCCAAGTACTCCAAGGGTAGATATGATGAAATTGTGAAGGAAGTTTCATCCTATTTGAAGAAGGTTGGCTATAACCCAGACAAAATTCCATTTGTTCCCATCTCTGGTTTTGAGGGAGATAACATGATTGAGCGCTCCACAAACCTTGACTGGTACAAGGGTCCAACCCTTCTTGAGGCCCTTGACCAAATCAATGAGCCTAAGAGGCCTTCAGACAAGCCCCTTCGACTACCACTTCAGGATGTCTACAAGATTGGAGGAATTGGAACTGTGCCTGTGGGGCGTGTCGAGACTGGTGTCATAAAACCTGGAATGGTTGTCACTTTTGCCCCTACTGGACTGACAACTGAAGTCAAGTCTGTGGAGATGCATCATGAAGCTCTAACAGAGGCCCTTCCTGGTGACAATGTGGGATTTAATGTCAAGAATGTTGCTGTTAAGGATCTCAAGCGTGGGTATGTTGCCTCAAACTCCAAGGATGACCCAGCCAAGGAGGCTGCTAGCTTTACTTCTCAAGTGATCATAATGAATCACCCTGGCCAGATTGGAAATGGCTATGCCCCTGTTCTTGACTGCCACACCTCTCACATTGCTGTCAAGTTTGCTGAGCTTGTTACTAAGATTGATAGGCGTTCTGGTAAGGAGATTGAGAAGGAGCCCAAATTCTTGAAGAATGGTGATGCTGGTATTATTAAGATGATTCCCACCAAGCCCATGGTGGTTGAGACTTTCTCTGAATATCCTCCACTCGGCCGTTTTGCTGTTAGAGACATGCGTCAAACTGTGGCTGTTGGCGTCATCAAGGCTGTGGAGAAGAAGGATCCCAGTGGAGGACTTAAGGTGACCAAGTCAGCATTGAAGAAGAAGTGA
Protein sequence

>Msa1444000

MGKEKVHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKGRYDEIVKEVSSYLKKVGYNPDKIPFVPISGFEGDNMIERSTNLDWYKGPTLLEALDQINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPTGLTTEVKSVEMHHEALTEALPGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKEAASFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFAELVTKIDRRSGKEIEKEPKFLKNGDAGIIKMIPTKPMVVETFSEYPPLGRFAVRDMRQTVAVGVIKAVEKKDPSGGLKVTKSALKKK*