AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004457

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004457.t1 MTR_2g020040 99.625 267 1 0 1 267 1 267 0.0 551
MS.gene004457.t1 MTR_4g057310 58.704 247 101 1 15 260 15 261 1.79e-110 320
MS.gene004457.t1 MTR_3g108240 58.065 248 103 1 12 258 58 305 3.23e-107 313
MS.gene004457.t1 MTR_1g047740 57.600 250 105 1 10 258 48 297 1.43e-106 311
MS.gene004457.t1 MTR_7g102990 54.800 250 112 1 10 258 16 265 7.69e-106 309
MS.gene004457.t1 MTR_3g108240 56.972 251 106 2 10 258 53 303 1.31e-104 306
MS.gene004457.t1 MTR_4g057310 59.000 200 81 1 15 213 18 217 9.27e-88 260
MS.gene004457.t1 MTR_1g010150 57.711 201 85 0 58 258 1 201 9.90e-87 257
MS.gene004457.t1 MTR_7g102990 54.545 176 79 1 10 184 16 191 1.64e-70 216
MS.gene004457.t1 MTR_8g104410 38.672 256 140 6 10 260 991 1234 7.26e-55 190
MS.gene004457.t1 MTR_8g104410 38.672 256 140 6 10 260 755 998 1.07e-54 190
MS.gene004457.t1 MTR_4g107440 40.000 250 133 6 16 260 1034 1271 6.20e-54 188
MS.gene004457.t1 MTR_6g083900 39.453 256 139 6 10 260 980 1224 3.59e-53 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004457.t1 AT5G18650 81.273 267 50 0 1 267 1 267 4.24e-169 468
MS.gene004457.t1 AT3G62970 60.985 264 101 2 4 266 20 282 3.46e-124 355
MS.gene004457.t1 AT5G22920 59.363 251 101 1 9 258 18 268 3.97e-112 325
MS.gene004457.t1 AT3G62970 61.333 225 86 1 4 227 20 244 1.30e-107 312
MS.gene004457.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene004457.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene004457.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene004457.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene004457.t1 AT5G25560 52.878 278 110 3 2 258 37 314 1.43e-102 301
MS.gene004457.t1 AT3G62970 60.648 216 84 1 4 218 20 235 1.55e-102 298
MS.gene004457.t1 AT5G25560 53.668 259 119 1 1 258 41 299 2.23e-100 295
MS.gene004457.t1 AT5G22920 58.182 220 91 1 9 227 18 237 1.43e-94 278
MS.gene004457.t1 AT3G18290 39.313 262 142 6 10 266 998 1247 1.69e-55 192
MS.gene004457.t1 AT1G18910 41.200 250 131 6 13 258 1015 1252 2.06e-55 192
MS.gene004457.t1 AT1G74770 38.554 249 137 6 16 260 1023 1259 4.90e-54 188
MS.gene004457.t1 AT1G74770 37.129 202 111 6 16 213 1023 1212 2.30e-39 146

Find 54 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCATTGAACGTCTTGATTT+TGG 0.056272 2.4:-66742508 MS.gene004457:CDS
GAAGTTTCCTTCCACATTAT+TGG 0.198378 2.4:-66739623 MS.gene004457:CDS
TTGATAGGTGTAAGCATTAT+AGG 0.242827 2.4:-66742377 MS.gene004457:intron
AAGTTTCCTTCCACATTATT+GGG 0.296468 2.4:-66739622 MS.gene004457:CDS
TATCTTTCAATGAGTCAAAA+AGG 0.335621 2.4:+66740474 None:intergenic
TGGAGTTCTCCACACATAAA+TGG 0.341398 2.4:+66740641 None:intergenic
GCATGGACCTCGTAACAAAT+TGG 0.347063 2.4:+66740604 None:intergenic
CTGTCATGCCTGATGATTAT+CGG 0.370354 2.4:-66740076 MS.gene004457:CDS
TTCGATTATTGCGGAAGAAC+AGG 0.378233 2.4:+66739551 None:intergenic
AATCACCGACTTGGAACATA+TGG 0.383543 2.4:+66740284 None:intergenic
CCCAAAATCAAGACGTTCAA+TGG 0.407306 2.4:+66742507 None:intergenic
ACAAGTTCGTGGTGATCAAA+AGG 0.413852 2.4:+66742164 None:intergenic
TTTCTATTCCGATAATCATC+AGG 0.448183 2.4:+66740068 None:intergenic
AGTTTCACTGTGACGATTGT+GGG 0.471619 2.4:-66741665 MS.gene004457:CDS
CAGTTTCACTGTGACGATTG+TGG 0.485260 2.4:-66741666 MS.gene004457:CDS
CGCATTTATGCCCAATAATG+TGG 0.502852 2.4:+66739612 None:intergenic
CACTAACTGTGGTGTCAGAA+TGG 0.512129 2.4:-66741883 MS.gene004457:CDS
CAAGTTCGTGGTGATCAAAA+GGG 0.517589 2.4:+66742165 None:intergenic
TAATTTGTAAACAGAGTTGG+TGG 0.530363 2.4:-66741563 MS.gene004457:intron
GTGACGATTGTGGGATCTGC+AGG 0.532433 2.4:-66741656 MS.gene004457:intron
ACATACCACACTTCTTGCAA+TGG 0.545335 2.4:+66741525 None:intergenic
ATCAAGACGTTCAATGGCAG+AGG 0.546666 2.4:+66742513 None:intergenic
AGACATGTCAATCACCGACT+TGG 0.549483 2.4:+66740275 None:intergenic
TCGTGCTCCTTGTTGCAATG+AGG 0.561125 2.4:-66742340 MS.gene004457:CDS
CATCATTACGAACAAGTTCG+TGG 0.564061 2.4:+66742153 None:intergenic
GTCGGTGATTGACATGTCTA+CGG 0.564442 2.4:-66740271 MS.gene004457:CDS
GATACAACCGTTATGAAATG+TGG 0.566189 2.4:-66740455 MS.gene004457:CDS
TGTGTGGCCACATTTCATAA+CGG 0.569384 2.4:+66740448 None:intergenic
CACATTATTGGGCATAAATG+CGG 0.569809 2.4:-66739611 MS.gene004457:CDS
ACTAACTGTGGTGTCAGAAT+GGG 0.571435 2.4:-66741882 MS.gene004457:CDS
AATTGGACAATGATGCCTCA+TGG 0.578817 2.4:+66740621 None:intergenic
TGTCACGCTTTATCATCTCA+TGG 0.581180 2.4:+66740408 None:intergenic
CTGCAAGAATTCGATTATTG+CGG 0.583669 2.4:+66739542 None:intergenic
TGATGATTATCGGAATAGAA+AGG 0.589941 2.4:-66740066 MS.gene004457:intron
TATTTCCATTGCAAGAAGTG+TGG 0.601945 2.4:-66741530 MS.gene004457:intron
TTTATGCCCAATAATGTGGA+AGG 0.608898 2.4:+66739616 None:intergenic
GCTCAAGTTTGCACTAACTG+TGG 0.612321 2.4:-66741894 MS.gene004457:CDS
ATCACCGACTTGGAACATAT+GGG 0.612737 2.4:+66740285 None:intergenic
AATAATGTGGAAGGAAACTT+CGG 0.612783 2.4:+66739625 None:intergenic
AGCATTATAGGAGAAGATGC+AGG 0.620867 2.4:-66742365 MS.gene004457:CDS
ATGGAAGAGAATTGATGAAG+AGG 0.624716 2.4:-66740247 MS.gene004457:intron
TGATTGACATGTCTACGGCA+TGG 0.625316 2.4:-66740266 MS.gene004457:CDS
GATTATTGCGGAAGAACAGG+AGG 0.633089 2.4:+66739554 None:intergenic
TCATTGTCCAATTTGTTACG+AGG 0.635894 2.4:-66740611 MS.gene004457:intron
GAGTAAACCTCATTGCAACA+AGG 0.647135 2.4:+66742333 None:intergenic
AATTTGTAAACAGAGTTGGT+GGG 0.647864 2.4:-66741562 MS.gene004457:intron
TCATTGTCATAACGAGGCAA+CGG 0.653857 2.4:-66742304 MS.gene004457:intron
TTGTCCCATATGTTCCAAGT+CGG 0.661678 2.4:-66740289 MS.gene004457:CDS
TTGCCTCGTTATGACAATGA+CGG 0.668381 2.4:+66742307 None:intergenic
CATCATCAAAGAATTTGCAG+AGG 0.687660 2.4:+66741844 None:intergenic
AACATTCACAATGCATTGTG+TGG 0.691022 2.4:+66740432 None:intergenic
GCGTGATAACCATTTATGTG+TGG 0.698690 2.4:-66740650 MS.gene004457:CDS
TTGCCGTCATTGTCATAACG+AGG 0.718027 2.4:-66742310 MS.gene004457:CDS
TATGTGTGGAGAACTCCATG+AGG 0.777816 2.4:-66740636 MS.gene004457:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATAAAAATGAAAACTTTA+TGG + chr2.4:66739625-66739644 None:intergenic 15.0%
!!! TTATTTGATTTTTGATTGAT+AGG - chr2.4:66739684-66739703 MS.gene004457:intron 15.0%
!!! TTTAAGCTTTTTAATTTTGT+GGG - chr2.4:66739658-66739677 MS.gene004457:CDS 15.0%
!!! TTTTAAGCTTTTTAATTTTG+TGG - chr2.4:66739657-66739676 MS.gene004457:CDS 15.0%
!! AACTTTATGGAAAATGAAAA+AGG + chr2.4:66739612-66739631 None:intergenic 20.0%
!! AATTATGAATAGATACATAC+TGG + chr2.4:66740087-66740106 None:intergenic 20.0%
!! AATTGAAAAACACAAATCAA+AGG + chr2.4:66739972-66739991 None:intergenic 20.0%
!! TAAATGAAAACATGGTATAA+GGG + chr2.4:66741511-66741530 None:intergenic 20.0%
!! TGTATTAAACTAGTATTACT+AGG + chr2.4:66742048-66742067 None:intergenic 20.0%
!! TTAAATGAAAACATGGTATA+AGG + chr2.4:66741512-66741531 None:intergenic 20.0%
!!! AGATTTTTAACATGAAAACA+AGG + chr2.4:66739824-66739843 None:intergenic 20.0%
!!! ATCTAATAAATTTATCGACT+TGG - chr2.4:66741327-66741346 MS.gene004457:intron 20.0%
!!! ATTTCTGTTTGATAATGTAT+TGG - chr2.4:66741028-66741047 MS.gene004457:intron 20.0%
!!! CAAAATCATTTTTCTATAGA+TGG - chr2.4:66740872-66740891 MS.gene004457:intron 20.0%
!!! CATCTATAGAAAAATGATTT+TGG + chr2.4:66740874-66740893 None:intergenic 20.0%
!!! TAATCTCATTGTTTTTGATT+TGG - chr2.4:66740347-66740366 MS.gene004457:intron 20.0%
!!! TTTCTGTTTGATAATGTATT+GGG - chr2.4:66741029-66741048 MS.gene004457:intron 20.0%
! ACTAAAGATGATAACATGTT+TGG + chr2.4:66740724-66740743 None:intergenic 25.0%
! ATGTTGAACAAGTAAGATTA+AGG - chr2.4:66739943-66739962 MS.gene004457:intron 25.0%
! ATGTTTGATGTCAAATATGA+AGG + chr2.4:66742113-66742132 None:intergenic 25.0%
! ATTTGAGTAAATAGTTTCTG+AGG - chr2.4:66740452-66740471 MS.gene004457:CDS 25.0%
! CAATTCTTTCATACTATCAA+AGG + chr2.4:66740701-66740720 None:intergenic 25.0%
! GTATGTATTTATTCATCACA+AGG - chr2.4:66740568-66740587 MS.gene004457:intron 25.0%
! TAGTTGCATCTCATAAATTT+GGG + chr2.4:66740481-66740500 None:intergenic 25.0%
! TATCTTTCAATGAGTCAAAA+AGG + chr2.4:66741605-66741624 None:intergenic 25.0%
! TATGTAATATTAACTCGACT+AGG - chr2.4:66741746-66741765 MS.gene004457:intron 25.0%
! TGTTGAACAAGTAAGATTAA+GGG - chr2.4:66739944-66739963 MS.gene004457:intron 25.0%
! TTAGTTGCATCTCATAAATT+TGG + chr2.4:66740482-66740501 None:intergenic 25.0%
! TTCTCTGAATTCATCTATTA+TGG - chr2.4:66741272-66741291 MS.gene004457:intron 25.0%
!! ACCATGTTTTCATTTAAACA+AGG - chr2.4:66741515-66741534 MS.gene004457:intron 25.0%
!! ATGTAATTTGTAAACAGAGT+TGG - chr2.4:66740510-66740529 MS.gene004457:intron 25.0%
!! CTCTTTAATGTTTCTTTTGA+AGG - chr2.4:66740157-66740176 MS.gene004457:intron 25.0%
!! TCCTTGTTTAAATGAAAACA+TGG + chr2.4:66741519-66741538 None:intergenic 25.0%
!! TGAAATCAGAATCCATTTTA+GGG - chr2.4:66741090-66741109 MS.gene004457:intron 25.0%
!! TTATGAGATGCAACTAATTT+TGG - chr2.4:66740485-66740504 MS.gene004457:intron 25.0%
!!! ATCATTTTTCTATAGATGGA+TGG - chr2.4:66740876-66740895 MS.gene004457:intron 25.0%
!!! TCTGTTGTGTTTTTGTTTTT+TGG - chr2.4:66740116-66740135 MS.gene004457:intron 25.0%
!!! TTATTGCTTTTTTCTGATCT+GGG - chr2.4:66739848-66739867 MS.gene004457:intron 25.0%
!!! TTGTGTTTTTGTTTTTTGGA+TGG - chr2.4:66740120-66740139 MS.gene004457:intron 25.0%
AAAACATGGTATAAGGGAAA+GGG + chr2.4:66741505-66741524 None:intergenic 30.0%
AAGTTTCCTTCCACATTATT+GGG - chr2.4:66742454-66742473 MS.gene004457:intron 30.0%
AATAATGTGGAAGGAAACTT+CGG + chr2.4:66742454-66742473 None:intergenic 30.0%
ACAAAGTGAAGTCAAATAGT+GGG + chr2.4:66742231-66742250 None:intergenic 30.0%
ATAACAAGAACAAGGGAAAA+AGG + chr2.4:66741066-66741085 None:intergenic 30.0%
GAATAAGGTTAGAAGTTCTA+TGG + chr2.4:66740953-66740972 None:intergenic 30.0%
GATATATGCATGTGAAAGAA+AGG + chr2.4:66742348-66742367 None:intergenic 30.0%
TACAAAGTGAAGTCAAATAG+TGG + chr2.4:66742232-66742251 None:intergenic 30.0%
TCATCGAAAACGAGAAAAAA+TGG + chr2.4:66740597-66740616 None:intergenic 30.0%
TCTTTCACATGCATATATCA+TGG - chr2.4:66742349-66742368 MS.gene004457:CDS 30.0%
TGATGATTATCGGAATAGAA+AGG - chr2.4:66742010-66742029 MS.gene004457:intron 30.0%
TTGACAATACTAAGCATGAA+TGG - chr2.4:66742313-66742332 MS.gene004457:CDS 30.0%
TTGACATCAAACATGCTTAT+GGG - chr2.4:66742119-66742138 MS.gene004457:intron 30.0%
TTGATAGGTGTAAGCATTAT+AGG - chr2.4:66739699-66739718 MS.gene004457:intron 30.0%
TTTCTATTCCGATAATCATC+AGG + chr2.4:66742011-66742030 None:intergenic 30.0%
TTTGACATCAAACATGCTTA+TGG - chr2.4:66742118-66742137 MS.gene004457:intron 30.0%
! AAAATGGATTCTGATTTCAG+AGG + chr2.4:66741089-66741108 None:intergenic 30.0%
! AATTTGTAAACAGAGTTGGT+GGG - chr2.4:66740514-66740533 MS.gene004457:intron 30.0%
! CTGAAATCAGAATCCATTTT+AGG - chr2.4:66741089-66741108 MS.gene004457:intron 30.0%
! TAATTTGTAAACAGAGTTGG+TGG - chr2.4:66740513-66740532 MS.gene004457:intron 30.0%
! TTTTATGCTCCACAATTATC+TGG - chr2.4:66741896-66741915 MS.gene004457:CDS 30.0%
!! AAATTTATCGACTTGGTTAG+TGG - chr2.4:66741334-66741353 MS.gene004457:intron 30.0%
!!! ATGGATTGTTTTGTTTCCAT+TGG - chr2.4:66741291-66741310 MS.gene004457:intron 30.0%
!!! CTTATTGCTTTTTTCTGATC+TGG - chr2.4:66739847-66739866 MS.gene004457:intron 30.0%
!!! GTTTTTTGAGAACCACTTTT+AGG + chr2.4:66741117-66741136 None:intergenic 30.0%
AACATTCACAATGCATTGTG+TGG + chr2.4:66741647-66741666 None:intergenic 35.0%
ATAAATTGCTCCATTTCGAC+AGG - chr2.4:66741382-66741401 MS.gene004457:intron 35.0%
ATGGAAGAGAATTGATGAAG+AGG - chr2.4:66741829-66741848 MS.gene004457:intron 35.0%
CACATTATTGGGCATAAATG+CGG - chr2.4:66742465-66742484 MS.gene004457:intron 35.0%
CAGCTGATAGAATCATCAAT+AGG + chr2.4:66740774-66740793 None:intergenic 35.0%
CATCATCAAAGAATTTGCAG+AGG + chr2.4:66740235-66740254 None:intergenic 35.0%
CATGTGAAAGAAAGGACAAA+AGG + chr2.4:66742340-66742359 None:intergenic 35.0%
GAAAACATGGTATAAGGGAA+AGG + chr2.4:66741506-66741525 None:intergenic 35.0%
GAAATCACTTGAGAACAAGT+TGG + chr2.4:66740975-66740994 None:intergenic 35.0%
GAAGTTTCCTTCCACATTAT+TGG - chr2.4:66742453-66742472 MS.gene004457:intron 35.0%
GATACAACCGTTATGAAATG+TGG - chr2.4:66741621-66741640 MS.gene004457:intron 35.0%
GTAACTACCATAGATCACAA+GGG + chr2.4:66741186-66741205 None:intergenic 35.0%
GTCAAATATGAAGGTTCTTG+CGG + chr2.4:66742104-66742123 None:intergenic 35.0%
TAAATTGCTCCATTTCGACA+GGG - chr2.4:66741383-66741402 MS.gene004457:intron 35.0%
TATTAACTCGACTAGGTGTA+TGG - chr2.4:66741753-66741772 MS.gene004457:intron 35.0%
TATTTCCATTGCAAGAAGTG+TGG - chr2.4:66740546-66740565 MS.gene004457:intron 35.0%
TCAGAGGATAACAAGAACAA+GGG + chr2.4:66741073-66741092 None:intergenic 35.0%
TGTTCTTGACCAGATAATTG+TGG + chr2.4:66741908-66741927 None:intergenic 35.0%
TTCAGAGGATAACAAGAACA+AGG + chr2.4:66741074-66741093 None:intergenic 35.0%
TTTATGCCCAATAATGTGGA+AGG + chr2.4:66742463-66742482 None:intergenic 35.0%
! CCATTGAACGTCTTGATTTT+GGG - chr2.4:66739569-66739588 MS.gene004457:CDS 35.0%
! CTTGAGAACAAGTTGGAATA+AGG + chr2.4:66740968-66740987 None:intergenic 35.0%
!! ACACACTGCTTTAGAAAACA+AGG + chr2.4:66740909-66740928 None:intergenic 35.0%
!! ACATGAGATGTTGATGATTG+TGG - chr2.4:66740005-66740024 MS.gene004457:intron 35.0%
!! ATTTTGGGAAGATGGGATAT+GGG - chr2.4:66739584-66739603 MS.gene004457:CDS 35.0%
!! TCATTGTCCAATTTGTTACG+AGG - chr2.4:66741465-66741484 MS.gene004457:intron 35.0%
!! TGTTTGCATCTTGTTGTTTC+AGG - chr2.4:66740032-66740051 MS.gene004457:intron 35.0%
!!! CCATGGATCAAGGTTTTTTT+GGG + chr2.4:66741208-66741227 None:intergenic 35.0%
!!! GCGTTTTGCGTTTTTTTGAA+AGG - chr2.4:66741227-66741246 MS.gene004457:intron 35.0%
!!! GTTTTGTTTCCATTGGACTT+TGG - chr2.4:66741298-66741317 MS.gene004457:intron 35.0%
AAGGAATTTCTACATGTCCC+TGG - chr2.4:66741534-66741553 MS.gene004457:CDS 40.0%
AAGTATCCAAACCTGCAACA+CGG + chr2.4:66742409-66742428 None:intergenic 40.0%
AATTGGACAATGATGCCTCA+TGG + chr2.4:66741458-66741477 None:intergenic 40.0%
ACAAGAACAAGGGAAAAAGG+AGG + chr2.4:66741063-66741082 None:intergenic 40.0%
ACAAGTTCGTGGTGATCAAA+AGG + chr2.4:66739915-66739934 None:intergenic 40.0%
ACATACCACACTTCTTGCAA+TGG + chr2.4:66740554-66740573 None:intergenic 40.0%
AGAATTGATGAAGAGGTAGC+TGG - chr2.4:66741836-66741855 MS.gene004457:intron 40.0%
AGCATTATAGGAGAAGATGC+AGG - chr2.4:66739711-66739730 MS.gene004457:intron 40.0%
AGTTTCACTGTGACGATTGT+GGG - chr2.4:66740411-66740430 MS.gene004457:CDS 40.0%
CAAAAAAACGCAAAACGCCA+TGG + chr2.4:66741225-66741244 None:intergenic 40.0%
CAACGCACAATTGACAACAT+TGG + chr2.4:66742150-66742169 None:intergenic 40.0%
CAAGTTCGTGGTGATCAAAA+GGG + chr2.4:66739914-66739933 None:intergenic 40.0%
CATCATTACGAACAAGTTCG+TGG + chr2.4:66739926-66739945 None:intergenic 40.0%
CCCAAAAAAACCTTGATCCA+TGG - chr2.4:66741205-66741224 MS.gene004457:intron 40.0%
CCCAAAATCAAGACGTTCAA+TGG + chr2.4:66739572-66739591 None:intergenic 40.0%
CGCATTTATGCCCAATAATG+TGG + chr2.4:66742467-66742486 None:intergenic 40.0%
CTGTCATGCCTGATGATTAT+CGG - chr2.4:66742000-66742019 MS.gene004457:intron 40.0%
GAGAAAACTATTGCCAACTG+AGG + chr2.4:66741257-66741276 None:intergenic 40.0%
GAGTAAACCTCATTGCAACA+AGG + chr2.4:66739746-66739765 None:intergenic 40.0%
GATATGACTCCAAAGTCCAA+TGG + chr2.4:66741310-66741329 None:intergenic 40.0%
GCGTGATAACCATTTATGTG+TGG - chr2.4:66741426-66741445 MS.gene004457:intron 40.0%
GGTAACTACCATAGATCACA+AGG + chr2.4:66741187-66741206 None:intergenic 40.0%
GTTCAAACCCTTGTGATCTA+TGG - chr2.4:66741176-66741195 MS.gene004457:intron 40.0%
TCATTGTCATAACGAGGCAA+CGG - chr2.4:66739772-66739791 MS.gene004457:intron 40.0%
TGGAGTTCTCCACACATAAA+TGG + chr2.4:66741438-66741457 None:intergenic 40.0%
TGTCACGCTTTATCATCTCA+TGG + chr2.4:66741671-66741690 None:intergenic 40.0%
TGTGTGGCCACATTTCATAA+CGG + chr2.4:66741631-66741650 None:intergenic 40.0%
TTGCCTCGTTATGACAATGA+CGG + chr2.4:66739772-66739791 None:intergenic 40.0%
TTGTCCCATATGTTCCAAGT+CGG - chr2.4:66741787-66741806 MS.gene004457:intron 40.0%
TTGTTCGTTCGATATGCAGA+CGG - chr2.4:66740379-66740398 MS.gene004457:intron 40.0%
! ACTAACTGTGGTGTCAGAAT+GGG - chr2.4:66740194-66740213 MS.gene004457:intron 40.0%
! GCCATTGAACGTCTTGATTT+TGG - chr2.4:66739568-66739587 MS.gene004457:CDS 40.0%
!! AATCACCGACTTGGAACATA+TGG + chr2.4:66741795-66741814 None:intergenic 40.0%
!! ACGTCTTGATTTTGGGAAGA+TGG - chr2.4:66739576-66739595 MS.gene004457:CDS 40.0%
!! ATCACCGACTTGGAACATAT+GGG + chr2.4:66741794-66741813 None:intergenic 40.0%
!! CCACTTTTAGGTCCCTAAAA+TGG + chr2.4:66741105-66741124 None:intergenic 40.0%
!! CCATTTTAGGGACCTAAAAG+TGG - chr2.4:66741102-66741121 MS.gene004457:intron 40.0%
!! CGTCTTGATTTTGGGAAGAT+GGG - chr2.4:66739577-66739596 MS.gene004457:CDS 40.0%
!! GATTTTGGGAAGATGGGATA+TGG - chr2.4:66739583-66739602 MS.gene004457:CDS 40.0%
!! TTTGAAAGGTTAGCCTCAGT+TGG - chr2.4:66741241-66741260 MS.gene004457:intron 40.0%
!!! GCCATGGATCAAGGTTTTTT+TGG + chr2.4:66741209-66741228 None:intergenic 40.0%
ACATGGTATAAGGGAAAGGG+AGG + chr2.4:66741502-66741521 None:intergenic 45.0%
AGAACAAGGGAAAAAGGAGG+TGG + chr2.4:66741060-66741079 None:intergenic 45.0%
AGAATACTCTCCTGCTTCCA+GGG + chr2.4:66741554-66741573 None:intergenic 45.0%
ATAGCAAGACCCTGTCGAAA+TGG + chr2.4:66741395-66741414 None:intergenic 45.0%
ATCAAGACGTTCAATGGCAG+AGG + chr2.4:66739566-66739585 None:intergenic 45.0%
CAGTTTCACTGTGACGATTG+TGG - chr2.4:66740410-66740429 MS.gene004457:CDS 45.0%
GCATGGACCTCGTAACAAAT+TGG + chr2.4:66741475-66741494 None:intergenic 45.0%
GCTCAAGTTTGCACTAACTG+TGG - chr2.4:66740182-66740201 MS.gene004457:intron 45.0%
GTCGGTGATTGACATGTCTA+CGG - chr2.4:66741805-66741824 MS.gene004457:intron 45.0%
TATGTGTGGAGAACTCCATG+AGG - chr2.4:66741440-66741459 MS.gene004457:intron 45.0%
TGATTGACATGTCTACGGCA+TGG - chr2.4:66741810-66741829 MS.gene004457:intron 45.0%
TGTTCGTTCGATATGCAGAC+GGG - chr2.4:66740380-66740399 MS.gene004457:intron 45.0%
TTGCCGTCATTGTCATAACG+AGG - chr2.4:66739766-66739785 MS.gene004457:intron 45.0%
! AGACATGTCAATCACCGACT+TGG + chr2.4:66741804-66741823 None:intergenic 45.0%
! CACTAACTGTGGTGTCAGAA+TGG - chr2.4:66740193-66740212 MS.gene004457:intron 45.0%
ACGCAAAACGCCATGGATCA+AGG + chr2.4:66741218-66741237 None:intergenic 50.0%
CAGAATACTCTCCTGCTTCC+AGG + chr2.4:66741555-66741574 None:intergenic 50.0%
CTAAAACAGCACCGTGTTGC+AGG - chr2.4:66742395-66742414 MS.gene004457:intron 50.0%
GTCTCATTGCATCAGCAAGC+AGG + chr2.4:66741006-66741025 None:intergenic 50.0%
GTTCGTTCGATATGCAGACG+GGG - chr2.4:66740381-66740400 MS.gene004457:intron 50.0%
TCGTGCTCCTTGTTGCAATG+AGG - chr2.4:66739736-66739755 MS.gene004457:intron 50.0%
TTCTACATGTCCCTGGAAGC+AGG - chr2.4:66741541-66741560 MS.gene004457:CDS 50.0%
ACAGCACCGTGTTGCAGGTT+TGG - chr2.4:66742400-66742419 MS.gene004457:intron 55.0%
AGGGAAAGGGAGGAAGAGCA+TGG + chr2.4:66741492-66741511 None:intergenic 55.0%
CTCGACTAGGTGTATGGCTC+AGG - chr2.4:66741759-66741778 MS.gene004457:intron 55.0%
GTGACGATTGTGGGATCTGC+AGG - chr2.4:66740420-66740439 MS.gene004457:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 66739556 66742542 66739556 ID=MS.gene004457
chr2.4 mRNA 66739556 66742542 66739556 ID=MS.gene004457.t1;Parent=MS.gene004457
chr2.4 exon 66742493 66742542 66742493 ID=MS.gene004457.t1.exon1;Parent=MS.gene004457.t1
chr2.4 CDS 66742493 66742542 66742493 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66742305 66742392 66742305 ID=MS.gene004457.t1.exon2;Parent=MS.gene004457.t1
chr2.4 CDS 66742305 66742392 66742305 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66742145 66742201 66742145 ID=MS.gene004457.t1.exon3;Parent=MS.gene004457.t1
chr2.4 CDS 66742145 66742201 66742145 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66742012 66742044 66742012 ID=MS.gene004457.t1.exon4;Parent=MS.gene004457.t1
chr2.4 CDS 66742012 66742044 66742012 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66741842 66741919 66741842 ID=MS.gene004457.t1.exon5;Parent=MS.gene004457.t1
chr2.4 CDS 66741842 66741919 66741842 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66741657 66741700 66741657 ID=MS.gene004457.t1.exon6;Parent=MS.gene004457.t1
chr2.4 CDS 66741657 66741700 66741657 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66741531 66741571 66741531 ID=MS.gene004457.t1.exon7;Parent=MS.gene004457.t1
chr2.4 CDS 66741531 66741571 66741531 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66740612 66740694 66740612 ID=MS.gene004457.t1.exon8;Parent=MS.gene004457.t1
chr2.4 CDS 66740612 66740694 66740612 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66740407 66740498 66740407 ID=MS.gene004457.t1.exon9;Parent=MS.gene004457.t1
chr2.4 CDS 66740407 66740498 66740407 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66740248 66740317 66740248 ID=MS.gene004457.t1.exon10;Parent=MS.gene004457.t1
chr2.4 CDS 66740248 66740317 66740248 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66740067 66740108 66740067 ID=MS.gene004457.t1.exon11;Parent=MS.gene004457.t1
chr2.4 CDS 66740067 66740108 66740067 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
chr2.4 exon 66739556 66739681 66739556 ID=MS.gene004457.t1.exon12;Parent=MS.gene004457.t1
chr2.4 CDS 66739556 66739681 66739556 ID=cds.MS.gene004457.t1;Parent=MS.gene004457.t1
Gene Sequence

>MS.gene004457

ATGGAAGCCTCTGCCATTGAACGTCTTGATTTTGGGAAGATGGGATATGGGTACCTTTTTCATTTTCCATAAAGTTTTCATTTTTATTCAATTTTCATTGTTTTTAAGCTTTTTAATTTTGTGGGTATTTATTTGATTTTTGATTGATAGGTGTAAGCATTATAGGAGAAGATGCAGGATTCGTGCTCCTTGTTGCAATGAGGTTTACTCTTGCCGTCATTGTCATAACGAGGCAACGGTATTTTTTTTTTTTTTGTTCTTTTTTCCTTGTTTTCATGTTAAAAATCTTGTCTTATTGCTTTTTTCTGATCTGGGTTTGTGTTATTTTTTTTCATTTTTAGAGCATGCTGAAAAACCCTTTTGATCACCACGAACTTGTTCGTAATGATGTTGAACAAGTAAGATTAAGGGCACCTTTGATTTGTGTTTTTCAATTACTGTGCAATTTTACATGAGATGTTGATGATTGTGGTTTTTGTTTGCATCTTGTTGTTTCAGGTTGTTTGTTCAGTTTGTGACACAGAGCAACCAGTATGTATCTATTCATAATTTGATTTGAATCTGTTGTGTTTTTGTTTTTTGGATGGATTTATGATGTTTTCTCTTTAATGTTTCTTTTGAAGGTTGCTCAAGTTTGCACTAACTGTGGTGTCAGAATGGGAGAATATTACTGTGACCTCTGCAAATTCTTTGATGATGATGTAATGATTTACTGAATAAATCTGCAGTATTTGATTTAAGTTTCTTCGTTTTTCGATATGATTGAATTTGAGAATATTGTTATTATTGATTAATCTCATTGTTTTTGATTTGGTGCTGATTGTTGTTCGTTCGATATGCAGACGGGGAAACAACAGTTTCACTGTGACGATTGTGGGATCTGCAGGTACAACTGTATTTGAGTAAATAGTTTCTGAGGTTTCCCAAATTTATGAGATGCAACTAATTTTGGTTATGTAATTTGTAAACAGAGTTGGTGGGAGTGAGAATTATTTCCATTGCAAGAAGTGTGGTATGTATTTATTCATCACAAGGCTGCCATTTTTTCTCGTTTTCGATGAAACATATATTTATTTTTTTCAAATTAGCTTATGCTGCAAATTGTTAATTTTCATATTAATAGATGTTTCAATTATCTACTTCCTTTGATAGTATGAAAGAATTGCCAAACATGTTATCATCTTTAGTGACACTAGAAGTTCAAAATCACTATTACCTATTGATGATTCTATCAGCTGTTTTCTTTTTTCTGTTACAGTTGAGTACTAATCATTTTCATTCTAACATATTAGCTTTTGTCTTATCATTTTAGCTTCCAAAATCATTTTTCTATAGATGGATGGAATGAAACCTTGTTTTCTAAAGCAGTGTGTGAATTTAATACTACATATATTCCATAGAACTTCTAACCTTATTCCAACTTGTTCTCAAGTGATTTCTTATTTTACCTGCTTGCTGATGCAATGAGACGCATTTCTGTTTGATAATGTATTGGGCTTTGCCACCTCCTTTTTCCCTTGTTCTTGTTATCCTCTGAAATCAGAATCCATTTTAGGGACCTAAAAGTGGTTCTCAAAAAACAAAGAATTCAGAATCTAGTGTATCAATTCAAATAGTATAGTTCAAACCCTTGTGATCTATGGTAGTTACCCAAAAAAACCTTGATCCATGGCGTTTTGCGTTTTTTTGAAAGGTTAGCCTCAGTTGGCAATAGTTTTCTCTGAATTCATCTATTATGGATTGTTTTGTTTCCATTGGACTTTGGAGTCATATCTAATAAATTTATCGACTTGGTTAGTGGAACTATTTTTTTCTTCTGTTTTAAAATAAATTGCTCCATTTCGACAGGGTCTTGCTATTCAGTTGCTCTGCGTGATAACCATTTATGTGTGGAGAACTCCATGAGGCATCATTGTCCAATTTGTTACGAGGTCCATGCTCTTCCTCCCTTTCCCTTATACCATGTTTTCATTTAAACAAGGAATTTCTACATGTCCCTGGAAGCAGGAGAGTATTCTGATGTGACTTTATTGTGTTTCTACAGTACCTTTTTGACTCATTGAAAGATACAACCGTTATGAAATGTGGCCACACAATGCATTGTGAATGTTACCATGAGATGATAAAGCGTGACAAGTGAGTATATTGCTTACTATTTGACAGTTATTTCGTTTATAAAGCATTATCTCTTATGTAATATTAACTCGACTAGGTGTATGGCTCAGGTACTGTTGTCCCATATGTTCCAAGTCGGTGATTGACATGTCTACGGCATGGAAGAGAATTGATGAAGAGGTAGCTGGTTAAATTACATGCATCTGTTTTCACTTGATTTTAAGTTTTTATGCTCCACAATTATCTGGTCAAGAACATTGAATATATATGTTCTTTACTACAATTGTGATTTCTAACTAATTTATTTTTGCTATATTAGATCGAAGCAACTGTCATGCCTGATGATTATCGGAATAGAAAGGTAAAACTATAGTCCTAGTAATACTAGTTTAATACATGCTATCTCTAATTGTCTACATTTCATTCACAGCCGCAAGAACCTTCATATTTGACATCAAACATGCTTATGGGCATGACCAATGTTGTCAATTGTGCGTTGCAGAAAATAGAGATTCGTTAAAATTTCGATACGCTACAGTGCTATAGCGTCGCTATAGCCCACTATTTGACTTCACTTTGTACTAAATAGCGTATTGCAAACGATAGTGATTTGTTCAAATTCTGTTTTGCTATAACTGTTATTTGACAATACTAAGCATGAATGGACCTTTTGTCCTTTCTTTCACATGCATATATCATGGTTATATTAGCAAATTTCTGAGAACTAAAACAGCACCGTGTTGCAGGTTTGGATACTTTGTAACGACTGCAACGATACAACCGAAGTTTCCTTCCACATTATTGGGCATAAATGCGGTCACTGCAGTTCATATAACACTCGTGCTATTGCTCCTCCTGTTCTTCCGCAATAA
Protein sequence

>MS.gene004457.t1

MEASAIERLDFGKMGYGCKHYRRRCRIRAPCCNEVYSCRHCHNEATSMLKNPFDHHELVRNDVEQVVCSVCDTEQPVAQVCTNCGVRMGEYYCDLCKFFDDDTGKQQFHCDDCGICRVGGSENYFHCKKCGSCYSVALRDNHLCVENSMRHHCPICYEYLFDSLKDTTVMKCGHTMHCECYHEMIKRDKYCCPICSKSVIDMSTAWKRIDEEIEATVMPDDYRNRKVWILCNDCNDTTEVSFHIIGHKCGHCSSYNTRAIAPPVLPQ