AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00991

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00991.t1 MTR_2g020040 99.625 267 1 0 1 267 1 267 0.0 551
MS.gene00991.t1 MTR_4g057310 58.704 247 101 1 15 260 15 261 1.79e-110 320
MS.gene00991.t1 MTR_3g108240 58.065 248 103 1 12 258 58 305 3.23e-107 313
MS.gene00991.t1 MTR_1g047740 57.600 250 105 1 10 258 48 297 1.43e-106 311
MS.gene00991.t1 MTR_7g102990 54.800 250 112 1 10 258 16 265 7.69e-106 309
MS.gene00991.t1 MTR_3g108240 56.972 251 106 2 10 258 53 303 1.31e-104 306
MS.gene00991.t1 MTR_4g057310 59.000 200 81 1 15 213 18 217 9.27e-88 260
MS.gene00991.t1 MTR_1g010150 57.711 201 85 0 58 258 1 201 9.90e-87 257
MS.gene00991.t1 MTR_7g102990 54.545 176 79 1 10 184 16 191 1.64e-70 216
MS.gene00991.t1 MTR_8g104410 38.672 256 140 6 10 260 991 1234 7.26e-55 190
MS.gene00991.t1 MTR_8g104410 38.672 256 140 6 10 260 755 998 1.07e-54 190
MS.gene00991.t1 MTR_4g107440 40.000 250 133 6 16 260 1034 1271 6.20e-54 188
MS.gene00991.t1 MTR_6g083900 39.453 256 139 6 10 260 980 1224 3.59e-53 186
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00991.t1 AT5G18650 81.273 267 50 0 1 267 1 267 4.24e-169 468
MS.gene00991.t1 AT3G62970 60.985 264 101 2 4 266 20 282 3.46e-124 355
MS.gene00991.t1 AT5G22920 59.363 251 101 1 9 258 18 268 3.97e-112 325
MS.gene00991.t1 AT3G62970 61.333 225 86 1 4 227 20 244 1.30e-107 312
MS.gene00991.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene00991.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene00991.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene00991.t1 AT5G25560 54.851 268 110 2 2 258 37 304 1.65e-105 308
MS.gene00991.t1 AT5G25560 52.878 278 110 3 2 258 37 314 1.43e-102 301
MS.gene00991.t1 AT3G62970 60.648 216 84 1 4 218 20 235 1.55e-102 298
MS.gene00991.t1 AT5G25560 53.668 259 119 1 1 258 41 299 2.23e-100 295
MS.gene00991.t1 AT5G22920 58.182 220 91 1 9 227 18 237 1.43e-94 278
MS.gene00991.t1 AT3G18290 39.313 262 142 6 10 266 998 1247 1.69e-55 192
MS.gene00991.t1 AT1G18910 41.200 250 131 6 13 258 1015 1252 2.06e-55 192
MS.gene00991.t1 AT1G74770 38.554 249 137 6 16 260 1023 1259 4.90e-54 188
MS.gene00991.t1 AT1G74770 37.129 202 111 6 16 213 1023 1212 2.30e-39 146

Find 54 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCATTGAACGTCTTGATTT+TGG 0.056272 2.3:-66950142 MS.gene00991:CDS
GAAGTTTCCTTCCACATTAT+TGG 0.198378 2.3:-66947259 MS.gene00991:CDS
TTGATAGGTGTAAGCATTAT+AGG 0.242827 2.3:-66950011 MS.gene00991:intron
AAGTTTCCTTCCACATTATT+GGG 0.296468 2.3:-66947258 MS.gene00991:CDS
TATCTTTCAATGAGTCAAAA+AGG 0.335621 2.3:+66948110 None:intergenic
TGGAGTTCTCCACACATAAA+TGG 0.341398 2.3:+66948277 None:intergenic
GCATGGACCTCGTAACAAAT+TGG 0.347063 2.3:+66948240 None:intergenic
CTGTCATGCCTGATGATTAT+CGG 0.370354 2.3:-66947712 MS.gene00991:CDS
TTCGATTATTGCGGAAGAAC+AGG 0.378233 2.3:+66947187 None:intergenic
AATCACCGACTTGGAACATA+TGG 0.383543 2.3:+66947920 None:intergenic
CCCAAAATCAAGACGTTCAA+TGG 0.407306 2.3:+66950141 None:intergenic
ACAAGTTCGTGGTGATCAAA+AGG 0.413852 2.3:+66949800 None:intergenic
TTTCTATTCCGATAATCATC+AGG 0.448183 2.3:+66947704 None:intergenic
AGTTTCACTGTGACGATTGT+GGG 0.471619 2.3:-66949301 MS.gene00991:CDS
CAGTTTCACTGTGACGATTG+TGG 0.485260 2.3:-66949302 MS.gene00991:CDS
CGCATTTATGCCCAATAATG+TGG 0.502852 2.3:+66947248 None:intergenic
CACTAACTGTGGTGTCAGAA+TGG 0.512129 2.3:-66949519 MS.gene00991:CDS
CAAGTTCGTGGTGATCAAAA+GGG 0.517589 2.3:+66949801 None:intergenic
TAATTTGTAAACAGAGTTGG+TGG 0.530363 2.3:-66949199 MS.gene00991:intron
GTGACGATTGTGGGATCTGC+AGG 0.532433 2.3:-66949292 MS.gene00991:intron
ACATACCACACTTCTTGCAA+TGG 0.545335 2.3:+66949161 None:intergenic
ATCAAGACGTTCAATGGCAG+AGG 0.546666 2.3:+66950147 None:intergenic
AGACATGTCAATCACCGACT+TGG 0.549483 2.3:+66947911 None:intergenic
TCGTGCTCCTTGTTGCAATG+AGG 0.561125 2.3:-66949974 MS.gene00991:CDS
CATCATTACGAACAAGTTCG+TGG 0.564061 2.3:+66949789 None:intergenic
GTCGGTGATTGACATGTCTA+CGG 0.564442 2.3:-66947907 MS.gene00991:CDS
GATACAACCGTTATGAAATG+TGG 0.566189 2.3:-66948091 MS.gene00991:CDS
TGTGTGGCCACATTTCATAA+CGG 0.569384 2.3:+66948084 None:intergenic
CACATTATTGGGCATAAATG+CGG 0.569809 2.3:-66947247 MS.gene00991:CDS
ACTAACTGTGGTGTCAGAAT+GGG 0.571435 2.3:-66949518 MS.gene00991:CDS
AATTGGACAATGATGCCTCA+TGG 0.578817 2.3:+66948257 None:intergenic
TGTCACGCTTTATCATCTCA+TGG 0.581180 2.3:+66948044 None:intergenic
CTGCAAGAATTCGATTATTG+CGG 0.583669 2.3:+66947178 None:intergenic
TGATGATTATCGGAATAGAA+AGG 0.589941 2.3:-66947702 MS.gene00991:intron
TATTTCCATTGCAAGAAGTG+TGG 0.601945 2.3:-66949166 MS.gene00991:intron
TTTATGCCCAATAATGTGGA+AGG 0.608898 2.3:+66947252 None:intergenic
GCTCAAGTTTGCACTAACTG+TGG 0.612321 2.3:-66949530 MS.gene00991:CDS
ATCACCGACTTGGAACATAT+GGG 0.612737 2.3:+66947921 None:intergenic
AATAATGTGGAAGGAAACTT+CGG 0.612783 2.3:+66947261 None:intergenic
AGCATTATAGGAGAAGATGC+AGG 0.620867 2.3:-66949999 MS.gene00991:CDS
ATGGAAGAGAATTGATGAAG+AGG 0.624716 2.3:-66947883 MS.gene00991:intron
TGATTGACATGTCTACGGCA+TGG 0.625316 2.3:-66947902 MS.gene00991:CDS
GATTATTGCGGAAGAACAGG+AGG 0.633089 2.3:+66947190 None:intergenic
TCATTGTCCAATTTGTTACG+AGG 0.635894 2.3:-66948247 MS.gene00991:intron
GAGTAAACCTCATTGCAACA+AGG 0.647135 2.3:+66949967 None:intergenic
AATTTGTAAACAGAGTTGGT+GGG 0.647864 2.3:-66949198 MS.gene00991:intron
TCATTGTCATAACGAGGCAA+CGG 0.653857 2.3:-66949938 MS.gene00991:intron
TTGTCCCATATGTTCCAAGT+CGG 0.661678 2.3:-66947925 MS.gene00991:CDS
TTGCCTCGTTATGACAATGA+CGG 0.668381 2.3:+66949941 None:intergenic
CATCATCAAAGAATTTGCAG+AGG 0.687660 2.3:+66949480 None:intergenic
AACATTCACAATGCATTGTG+TGG 0.691022 2.3:+66948068 None:intergenic
GCGTGATAACCATTTATGTG+TGG 0.698690 2.3:-66948286 MS.gene00991:CDS
TTGCCGTCATTGTCATAACG+AGG 0.718027 2.3:-66949944 MS.gene00991:CDS
TATGTGTGGAGAACTCCATG+AGG 0.777816 2.3:-66948272 MS.gene00991:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATAAAAATGAAAACTTTA+TGG + chr2.3:66947261-66947280 None:intergenic 15.0%
!!! TTATTTGATTTTTGATTGAT+AGG - chr2.3:66947320-66947339 MS.gene00991:intron 15.0%
!!! TTTAAGCTTTTTAATTTTGT+GGG - chr2.3:66947294-66947313 MS.gene00991:CDS 15.0%
!!! TTTTAAGCTTTTTAATTTTG+TGG - chr2.3:66947293-66947312 MS.gene00991:CDS 15.0%
!! AACTTTATGGAAAATGAAAA+AGG + chr2.3:66947248-66947267 None:intergenic 20.0%
!! AATTATGAATAGATACATAC+TGG + chr2.3:66947721-66947740 None:intergenic 20.0%
!! AATTGAAAAACACAAATCAA+AGG + chr2.3:66947606-66947625 None:intergenic 20.0%
!! TAAATGAAAACATGGTATAA+GGG + chr2.3:66949145-66949164 None:intergenic 20.0%
!! TGTATTAAACTAGTATTACT+AGG + chr2.3:66949682-66949701 None:intergenic 20.0%
!! TTAAATGAAAACATGGTATA+AGG + chr2.3:66949146-66949165 None:intergenic 20.0%
!!! AGATTTTTAACATGAAAACA+AGG + chr2.3:66947458-66947477 None:intergenic 20.0%
!!! ATCTAATAAATTTATCGACT+TGG - chr2.3:66948961-66948980 MS.gene00991:intron 20.0%
!!! ATTTCTGTTTGATAATGTAT+TGG - chr2.3:66948662-66948681 MS.gene00991:intron 20.0%
!!! CAAAATCATTTTTCTATAGA+TGG - chr2.3:66948506-66948525 MS.gene00991:intron 20.0%
!!! CATCTATAGAAAAATGATTT+TGG + chr2.3:66948508-66948527 None:intergenic 20.0%
!!! TAATCTCATTGTTTTTGATT+TGG - chr2.3:66947981-66948000 MS.gene00991:intron 20.0%
!!! TTTCTGTTTGATAATGTATT+GGG - chr2.3:66948663-66948682 MS.gene00991:intron 20.0%
! ACTAAAGATGATAACATGTT+TGG + chr2.3:66948358-66948377 None:intergenic 25.0%
! ATGTTGAACAAGTAAGATTA+AGG - chr2.3:66947577-66947596 MS.gene00991:intron 25.0%
! ATGTTTGATGTCAAATATGA+AGG + chr2.3:66949747-66949766 None:intergenic 25.0%
! ATTTGAGTAAATAGTTTCTG+AGG - chr2.3:66948086-66948105 MS.gene00991:CDS 25.0%
! CAATTCTTTCATACTATCAA+AGG + chr2.3:66948335-66948354 None:intergenic 25.0%
! GTATGTATTTATTCATCACA+AGG - chr2.3:66948202-66948221 MS.gene00991:intron 25.0%
! TAGTTGCATCTCATAAATTT+GGG + chr2.3:66948115-66948134 None:intergenic 25.0%
! TATCTTTCAATGAGTCAAAA+AGG + chr2.3:66949239-66949258 None:intergenic 25.0%
! TGTTGAACAAGTAAGATTAA+GGG - chr2.3:66947578-66947597 MS.gene00991:intron 25.0%
! TTAGTTGCATCTCATAAATT+TGG + chr2.3:66948116-66948135 None:intergenic 25.0%
! TTCTCTGAATTCATCTATTA+TGG - chr2.3:66948906-66948925 MS.gene00991:intron 25.0%
! TTTGTAATATTAACTCGACT+AGG - chr2.3:66949380-66949399 MS.gene00991:intron 25.0%
!! ACCATGTTTTCATTTAAACA+AGG - chr2.3:66949149-66949168 MS.gene00991:intron 25.0%
!! ATGTAATTTGTAAACAGAGT+TGG - chr2.3:66948144-66948163 MS.gene00991:intron 25.0%
!! CTCTTTAATGTTTCTTTTGA+AGG - chr2.3:66947791-66947810 MS.gene00991:intron 25.0%
!! TCCTTGTTTAAATGAAAACA+TGG + chr2.3:66949153-66949172 None:intergenic 25.0%
!! TGAAATCAGAATCCATTTTA+GGG - chr2.3:66948724-66948743 MS.gene00991:intron 25.0%
!! TTATGAGATGCAACTAATTT+TGG - chr2.3:66948119-66948138 MS.gene00991:intron 25.0%
!!! ATCATTTTTCTATAGATGGA+TGG - chr2.3:66948510-66948529 MS.gene00991:intron 25.0%
!!! TCTGTTGTGTTTTTGTTTTT+TGG - chr2.3:66947750-66947769 MS.gene00991:intron 25.0%
!!! TTATTGCTTTTTTCTGATCT+GGG - chr2.3:66947482-66947501 MS.gene00991:intron 25.0%
!!! TTGTGTTTTTGTTTTTTGGA+TGG - chr2.3:66947754-66947773 MS.gene00991:intron 25.0%
AAAACATGGTATAAGGGAAA+GGG + chr2.3:66949139-66949158 None:intergenic 30.0%
AAGTTTCCTTCCACATTATT+GGG - chr2.3:66950088-66950107 MS.gene00991:intron 30.0%
AATAATGTGGAAGGAAACTT+CGG + chr2.3:66950088-66950107 None:intergenic 30.0%
ACAAAGTGAAGTCAAATAGT+GGG + chr2.3:66949865-66949884 None:intergenic 30.0%
ATAACAAGAACAAGGGAAAA+AGG + chr2.3:66948700-66948719 None:intergenic 30.0%
GAATAAGGTTAGAAGTTCTA+TGG + chr2.3:66948587-66948606 None:intergenic 30.0%
GATATATGCATGTGAAAGAA+AGG + chr2.3:66949982-66950001 None:intergenic 30.0%
TACAAAGTGAAGTCAAATAG+TGG + chr2.3:66949866-66949885 None:intergenic 30.0%
TCATCGAAAACGAGAAAAAA+TGG + chr2.3:66948231-66948250 None:intergenic 30.0%
TCTTTCACATGCATATATCA+TGG - chr2.3:66949983-66950002 MS.gene00991:CDS 30.0%
TGATGATTATCGGAATAGAA+AGG - chr2.3:66949644-66949663 MS.gene00991:intron 30.0%
TTGACAATACTAAGCATGAA+TGG - chr2.3:66949947-66949966 MS.gene00991:CDS 30.0%
TTGACATCAAACATGCTTAT+GGG - chr2.3:66949753-66949772 MS.gene00991:intron 30.0%
TTGATAGGTGTAAGCATTAT+AGG - chr2.3:66947335-66947354 MS.gene00991:intron 30.0%
TTTCTATTCCGATAATCATC+AGG + chr2.3:66949645-66949664 None:intergenic 30.0%
TTTGACATCAAACATGCTTA+TGG - chr2.3:66949752-66949771 MS.gene00991:intron 30.0%
! AAAATGGATTCTGATTTCAG+AGG + chr2.3:66948723-66948742 None:intergenic 30.0%
! AATTTGTAAACAGAGTTGGT+GGG - chr2.3:66948148-66948167 MS.gene00991:intron 30.0%
! CTGAAATCAGAATCCATTTT+AGG - chr2.3:66948723-66948742 MS.gene00991:intron 30.0%
! TAATTTGTAAACAGAGTTGG+TGG - chr2.3:66948147-66948166 MS.gene00991:intron 30.0%
! TTTTATGCTCCACAATTATC+TGG - chr2.3:66949530-66949549 MS.gene00991:CDS 30.0%
!! AAATTTATCGACTTGGTTAG+TGG - chr2.3:66948968-66948987 MS.gene00991:intron 30.0%
!!! ATGGATTGTTTTGTTTCCAT+TGG - chr2.3:66948925-66948944 MS.gene00991:intron 30.0%
!!! CTTATTGCTTTTTTCTGATC+TGG - chr2.3:66947481-66947500 MS.gene00991:intron 30.0%
!!! GTTTTTTGAGAACCACTTTT+AGG + chr2.3:66948751-66948770 None:intergenic 30.0%
AACATTCACAATGCATTGTG+TGG + chr2.3:66949281-66949300 None:intergenic 35.0%
ATAAATTGCTCCATTTCGAC+AGG - chr2.3:66949016-66949035 MS.gene00991:intron 35.0%
ATGGAAGAGAATTGATGAAG+AGG - chr2.3:66949463-66949482 MS.gene00991:intron 35.0%
CACATTATTGGGCATAAATG+CGG - chr2.3:66950099-66950118 MS.gene00991:intron 35.0%
CAGCTGATAGAATCATCAAT+AGG + chr2.3:66948408-66948427 None:intergenic 35.0%
CATCATCAAAGAATTTGCAG+AGG + chr2.3:66947869-66947888 None:intergenic 35.0%
CATGTGAAAGAAAGGACAAA+AGG + chr2.3:66949974-66949993 None:intergenic 35.0%
GAAAACATGGTATAAGGGAA+AGG + chr2.3:66949140-66949159 None:intergenic 35.0%
GAAATCACTTGAGAACAAGT+TGG + chr2.3:66948609-66948628 None:intergenic 35.0%
GAAGTTTCCTTCCACATTAT+TGG - chr2.3:66950087-66950106 MS.gene00991:intron 35.0%
GATACAACCGTTATGAAATG+TGG - chr2.3:66949255-66949274 MS.gene00991:intron 35.0%
GTAACTACCATAGATCACAA+GGG + chr2.3:66948820-66948839 None:intergenic 35.0%
GTCAAATATGAAGGTTCTTG+CGG + chr2.3:66949738-66949757 None:intergenic 35.0%
TAAATTGCTCCATTTCGACA+GGG - chr2.3:66949017-66949036 MS.gene00991:intron 35.0%
TATTAACTCGACTAGGTGTA+TGG - chr2.3:66949387-66949406 MS.gene00991:intron 35.0%
TATTTCCATTGCAAGAAGTG+TGG - chr2.3:66948180-66948199 MS.gene00991:intron 35.0%
TCAGAGGATAACAAGAACAA+GGG + chr2.3:66948707-66948726 None:intergenic 35.0%
TGTTCTTGACCAGATAATTG+TGG + chr2.3:66949542-66949561 None:intergenic 35.0%
TTCAGAGGATAACAAGAACA+AGG + chr2.3:66948708-66948727 None:intergenic 35.0%
TTTATGCCCAATAATGTGGA+AGG + chr2.3:66950097-66950116 None:intergenic 35.0%
! CCATTGAACGTCTTGATTTT+GGG - chr2.3:66947205-66947224 MS.gene00991:CDS 35.0%
! CTTGAGAACAAGTTGGAATA+AGG + chr2.3:66948602-66948621 None:intergenic 35.0%
!! ACACACTGCTTTAGAAAACA+AGG + chr2.3:66948543-66948562 None:intergenic 35.0%
!! ACATGAGATGTTGATGATTG+TGG - chr2.3:66947639-66947658 MS.gene00991:intron 35.0%
!! ATTTTGGGAAGATGGGATAT+GGG - chr2.3:66947220-66947239 MS.gene00991:CDS 35.0%
!! TCATTGTCCAATTTGTTACG+AGG - chr2.3:66949099-66949118 MS.gene00991:intron 35.0%
!! TGTTTGCATCTTGTTGTTTC+AGG - chr2.3:66947666-66947685 MS.gene00991:intron 35.0%
!!! CCATGGATCAAGGTTTTTTT+GGG + chr2.3:66948842-66948861 None:intergenic 35.0%
!!! GCGTTTTGCGTTTTTTTGAA+AGG - chr2.3:66948861-66948880 MS.gene00991:intron 35.0%
!!! GTTTTGTTTCCATTGGACTT+TGG - chr2.3:66948932-66948951 MS.gene00991:intron 35.0%
AAGGAATTTCTACATGTCCC+TGG - chr2.3:66949168-66949187 MS.gene00991:CDS 40.0%
AAGTATCCAAACCTGCAACA+CGG + chr2.3:66950043-66950062 None:intergenic 40.0%
AATTGGACAATGATGCCTCA+TGG + chr2.3:66949092-66949111 None:intergenic 40.0%
ACAAGAACAAGGGAAAAAGG+AGG + chr2.3:66948697-66948716 None:intergenic 40.0%
ACAAGTTCGTGGTGATCAAA+AGG + chr2.3:66947549-66947568 None:intergenic 40.0%
ACATACCACACTTCTTGCAA+TGG + chr2.3:66948188-66948207 None:intergenic 40.0%
AGAATTGATGAAGAGGTAGC+TGG - chr2.3:66949470-66949489 MS.gene00991:intron 40.0%
AGCATTATAGGAGAAGATGC+AGG - chr2.3:66947347-66947366 MS.gene00991:intron 40.0%
AGTTTCACTGTGACGATTGT+GGG - chr2.3:66948045-66948064 MS.gene00991:CDS 40.0%
CAAAAAAACGCAAAACGCCA+TGG + chr2.3:66948859-66948878 None:intergenic 40.0%
CAACGCACAATTGACAACAT+TGG + chr2.3:66949784-66949803 None:intergenic 40.0%
CAAGTTCGTGGTGATCAAAA+GGG + chr2.3:66947548-66947567 None:intergenic 40.0%
CATCATTACGAACAAGTTCG+TGG + chr2.3:66947560-66947579 None:intergenic 40.0%
CCCAAAAAAACCTTGATCCA+TGG - chr2.3:66948839-66948858 MS.gene00991:intron 40.0%
CCCAAAATCAAGACGTTCAA+TGG + chr2.3:66947208-66947227 None:intergenic 40.0%
CGCATTTATGCCCAATAATG+TGG + chr2.3:66950101-66950120 None:intergenic 40.0%
CTGTCATGCCTGATGATTAT+CGG - chr2.3:66949634-66949653 MS.gene00991:intron 40.0%
GAGAAAACTATTGCCAACTG+AGG + chr2.3:66948891-66948910 None:intergenic 40.0%
GAGTAAACCTCATTGCAACA+AGG + chr2.3:66947382-66947401 None:intergenic 40.0%
GATATGACTCCAAAGTCCAA+TGG + chr2.3:66948944-66948963 None:intergenic 40.0%
GCGTGATAACCATTTATGTG+TGG - chr2.3:66949060-66949079 MS.gene00991:intron 40.0%
GGTAACTACCATAGATCACA+AGG + chr2.3:66948821-66948840 None:intergenic 40.0%
GTTCAAACCCTTGTGATCTA+TGG - chr2.3:66948810-66948829 MS.gene00991:intron 40.0%
TCATTGTCATAACGAGGCAA+CGG - chr2.3:66947408-66947427 MS.gene00991:intron 40.0%
TGGAGTTCTCCACACATAAA+TGG + chr2.3:66949072-66949091 None:intergenic 40.0%
TGTCACGCTTTATCATCTCA+TGG + chr2.3:66949305-66949324 None:intergenic 40.0%
TGTGTGGCCACATTTCATAA+CGG + chr2.3:66949265-66949284 None:intergenic 40.0%
TTGCCTCGTTATGACAATGA+CGG + chr2.3:66947408-66947427 None:intergenic 40.0%
TTGTCCCATATGTTCCAAGT+CGG - chr2.3:66949421-66949440 MS.gene00991:intron 40.0%
TTGTTCGTTCGATATGCAGA+CGG - chr2.3:66948013-66948032 MS.gene00991:intron 40.0%
! ACTAACTGTGGTGTCAGAAT+GGG - chr2.3:66947828-66947847 MS.gene00991:intron 40.0%
! GCCATTGAACGTCTTGATTT+TGG - chr2.3:66947204-66947223 MS.gene00991:CDS 40.0%
!! AATCACCGACTTGGAACATA+TGG + chr2.3:66949429-66949448 None:intergenic 40.0%
!! ACGTCTTGATTTTGGGAAGA+TGG - chr2.3:66947212-66947231 MS.gene00991:CDS 40.0%
!! ATCACCGACTTGGAACATAT+GGG + chr2.3:66949428-66949447 None:intergenic 40.0%
!! CCACTTTTAGGTCCCTAAAA+TGG + chr2.3:66948739-66948758 None:intergenic 40.0%
!! CCATTTTAGGGACCTAAAAG+TGG - chr2.3:66948736-66948755 MS.gene00991:intron 40.0%
!! CGTCTTGATTTTGGGAAGAT+GGG - chr2.3:66947213-66947232 MS.gene00991:CDS 40.0%
!! GATTTTGGGAAGATGGGATA+TGG - chr2.3:66947219-66947238 MS.gene00991:CDS 40.0%
!! TTTGAAAGGTTAGCCTCAGT+TGG - chr2.3:66948875-66948894 MS.gene00991:intron 40.0%
!!! GCCATGGATCAAGGTTTTTT+TGG + chr2.3:66948843-66948862 None:intergenic 40.0%
ACATGGTATAAGGGAAAGGG+AGG + chr2.3:66949136-66949155 None:intergenic 45.0%
AGAACAAGGGAAAAAGGAGG+TGG + chr2.3:66948694-66948713 None:intergenic 45.0%
AGAATACTCTCCTGCTTCCA+GGG + chr2.3:66949188-66949207 None:intergenic 45.0%
ATAGCAAGACCCTGTCGAAA+TGG + chr2.3:66949029-66949048 None:intergenic 45.0%
ATCAAGACGTTCAATGGCAG+AGG + chr2.3:66947202-66947221 None:intergenic 45.0%
CAGTTTCACTGTGACGATTG+TGG - chr2.3:66948044-66948063 MS.gene00991:CDS 45.0%
GCATGGACCTCGTAACAAAT+TGG + chr2.3:66949109-66949128 None:intergenic 45.0%
GCTCAAGTTTGCACTAACTG+TGG - chr2.3:66947816-66947835 MS.gene00991:intron 45.0%
GTCGGTGATTGACATGTCTA+CGG - chr2.3:66949439-66949458 MS.gene00991:intron 45.0%
TATGTGTGGAGAACTCCATG+AGG - chr2.3:66949074-66949093 MS.gene00991:intron 45.0%
TGATTGACATGTCTACGGCA+TGG - chr2.3:66949444-66949463 MS.gene00991:intron 45.0%
TGTTCGTTCGATATGCAGAC+GGG - chr2.3:66948014-66948033 MS.gene00991:intron 45.0%
TTGCCGTCATTGTCATAACG+AGG - chr2.3:66947402-66947421 MS.gene00991:intron 45.0%
! AGACATGTCAATCACCGACT+TGG + chr2.3:66949438-66949457 None:intergenic 45.0%
! CACTAACTGTGGTGTCAGAA+TGG - chr2.3:66947827-66947846 MS.gene00991:intron 45.0%
ACGCAAAACGCCATGGATCA+AGG + chr2.3:66948852-66948871 None:intergenic 50.0%
CAGAATACTCTCCTGCTTCC+AGG + chr2.3:66949189-66949208 None:intergenic 50.0%
CTAAAACAGCACCGTGTTGC+AGG - chr2.3:66950029-66950048 MS.gene00991:intron 50.0%
GTCTCATTGCATCAGCAAGC+AGG + chr2.3:66948640-66948659 None:intergenic 50.0%
GTTCGTTCGATATGCAGACG+GGG - chr2.3:66948015-66948034 MS.gene00991:intron 50.0%
TCGTGCTCCTTGTTGCAATG+AGG - chr2.3:66947372-66947391 MS.gene00991:intron 50.0%
TTCTACATGTCCCTGGAAGC+AGG - chr2.3:66949175-66949194 MS.gene00991:CDS 50.0%
ACAGCACCGTGTTGCAGGTT+TGG - chr2.3:66950034-66950053 MS.gene00991:intron 55.0%
AGGGAAAGGGAGGAAGAGCA+TGG + chr2.3:66949126-66949145 None:intergenic 55.0%
CTCGACTAGGTGTATGGCTC+AGG - chr2.3:66949393-66949412 MS.gene00991:intron 55.0%
GTGACGATTGTGGGATCTGC+AGG - chr2.3:66948054-66948073 MS.gene00991:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 66947192 66950176 66947192 ID=MS.gene00991
chr2.3 mRNA 66947192 66950176 66947192 ID=MS.gene00991.t1;Parent=MS.gene00991
chr2.3 exon 66950127 66950176 66950127 ID=MS.gene00991.t1.exon1;Parent=MS.gene00991.t1
chr2.3 CDS 66950127 66950176 66950127 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66949939 66950026 66949939 ID=MS.gene00991.t1.exon2;Parent=MS.gene00991.t1
chr2.3 CDS 66949939 66950026 66949939 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66949781 66949837 66949781 ID=MS.gene00991.t1.exon3;Parent=MS.gene00991.t1
chr2.3 CDS 66949781 66949837 66949781 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66949648 66949680 66949648 ID=MS.gene00991.t1.exon4;Parent=MS.gene00991.t1
chr2.3 CDS 66949648 66949680 66949648 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66949478 66949555 66949478 ID=MS.gene00991.t1.exon5;Parent=MS.gene00991.t1
chr2.3 CDS 66949478 66949555 66949478 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66949293 66949336 66949293 ID=MS.gene00991.t1.exon6;Parent=MS.gene00991.t1
chr2.3 CDS 66949293 66949336 66949293 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66949167 66949207 66949167 ID=MS.gene00991.t1.exon7;Parent=MS.gene00991.t1
chr2.3 CDS 66949167 66949207 66949167 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66948248 66948330 66948248 ID=MS.gene00991.t1.exon8;Parent=MS.gene00991.t1
chr2.3 CDS 66948248 66948330 66948248 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66948043 66948134 66948043 ID=MS.gene00991.t1.exon9;Parent=MS.gene00991.t1
chr2.3 CDS 66948043 66948134 66948043 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66947884 66947953 66947884 ID=MS.gene00991.t1.exon10;Parent=MS.gene00991.t1
chr2.3 CDS 66947884 66947953 66947884 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66947703 66947744 66947703 ID=MS.gene00991.t1.exon11;Parent=MS.gene00991.t1
chr2.3 CDS 66947703 66947744 66947703 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
chr2.3 exon 66947192 66947317 66947192 ID=MS.gene00991.t1.exon12;Parent=MS.gene00991.t1
chr2.3 CDS 66947192 66947317 66947192 ID=cds.MS.gene00991.t1;Parent=MS.gene00991.t1
Gene Sequence

>MS.gene00991

ATGGAAGCCTCTGCCATTGAACGTCTTGATTTTGGGAAGATGGGATATGGGTACCTTTTTCATTTTCCATAAAGTTTTCATTTTTATTCAATTTTCATTGTTTTTAAGCTTTTTAATTTTGTGGGTATTTATTTGATTTTTGATTGATAGGTGTAAGCATTATAGGAGAAGATGCAGGATTCGTGCTCCTTGTTGCAATGAGGTTTACTCTTGCCGTCATTGTCATAACGAGGCAACGGTATTTTTTTTTTTTGTTCTTTTTTCCTTGTTTTCATGTTAAAAATCTTGTCTTATTGCTTTTTTCTGATCTGGGTTTGTGTTATTTTTTTTCATTTTTAGAGCATGCTGAAAAACCCTTTTGATCACCACGAACTTGTTCGTAATGATGTTGAACAAGTAAGATTAAGGGCACCTTTGATTTGTGTTTTTCAATTACTGTGCAATTTTACATGAGATGTTGATGATTGTGGTTTTTGTTTGCATCTTGTTGTTTCAGGTTGTTTGTTCAGTTTGTGACACAGAGCAACCAGTATGTATCTATTCATAATTTGATTTGAATCTGTTGTGTTTTTGTTTTTTGGATGGATTTATGATGTTTTCTCTTTAATGTTTCTTTTGAAGGTTGCTCAAGTTTGCACTAACTGTGGTGTCAGAATGGGAGAATATTACTGTGACCTCTGCAAATTCTTTGATGATGATGTAATGATTTACTGAATAAATCTGCAGTATTTGATTTAAGTTTCTTCGTTTTTCGATATGATTGAATTTGAGAATATTGTTATTATTGATTAATCTCATTGTTTTTGATTTGGAGCTGATTGTTGTTCGTTCGATATGCAGACGGGGAAACAACAGTTTCACTGTGACGATTGTGGGATCTGCAGGTACAACTGTATTTGAGTAAATAGTTTCTGAGGTTTCCCAAATTTATGAGATGCAACTAATTTTGGTTATGTAATTTGTAAACAGAGTTGGTGGGAGTGAGAATTATTTCCATTGCAAGAAGTGTGGTATGTATTTATTCATCACAAGGCTGCCATTTTTTCTCGTTTTCGATGAAACATATATTTATTTTTTTCAAATTAGCTTATGCTGCAAATTGTTAATTTTCATATTAATAGATGTTTCAATTATCTACTTCCTTTGATAGTATGAAAGAATTGCCAAACATGTTATCATCTTTAGTGACACTAGAAGTTCAAAATCACTATTACCTATTGATGATTCTATCAGCTGTTTTCTTTTTTCTGTTACAGTTGAGTACTAATCATTTTCATTCTAACATATTAGCTTTTGTCTTATCATTTTAGCTTCCAAAATCATTTTTCTATAGATGGATGGAATGAAACCTTGTTTTCTAAAGCAGTGTGTGAATTTAATACTACATATATTCCATAGAACTTCTAACCTTATTCCAACTTGTTCTCAAGTGATTTCTTATTTTACCTGCTTGCTGATGCAATGAGACGCATTTCTGTTTGATAATGTATTGGGCTTTGCCACCTCCTTTTTCCCTTGTTCTTGTTATCCTCTGAAATCAGAATCCATTTTAGGGACCTAAAAGTGGTTCTCAAAAAACAAAGAATTCAGAATCTAGTGTATCAATTCAAATAGTATAGTTCAAACCCTTGTGATCTATGGTAGTTACCCAAAAAAACCTTGATCCATGGCGTTTTGCGTTTTTTTGAAAGGTTAGCCTCAGTTGGCAATAGTTTTCTCTGAATTCATCTATTATGGATTGTTTTGTTTCCATTGGACTTTGGAGTCATATCTAATAAATTTATCGACTTGGTTAGTGGAACTATTTTTTTCTTCTGTTTTAAAATAAATTGCTCCATTTCGACAGGGTCTTGCTATTCAGTTGCTCTGCGTGATAACCATTTATGTGTGGAGAACTCCATGAGGCATCATTGTCCAATTTGTTACGAGGTCCATGCTCTTCCTCCCTTTCCCTTATACCATGTTTTCATTTAAACAAGGAATTTCTACATGTCCCTGGAAGCAGGAGAGTATTCTGATGTGACTTTATTGTGTTTCTACAGTACCTTTTTGACTCATTGAAAGATACAACCGTTATGAAATGTGGCCACACAATGCATTGTGAATGTTACCATGAGATGATAAAGCGTGACAAGTGAGTATATTGCTTACTATTTGACAGTTATTTCGTTTATAAAGCATTATCTCTTTTGTAATATTAACTCGACTAGGTGTATGGCTCAGGTACTGTTGTCCCATATGTTCCAAGTCGGTGATTGACATGTCTACGGCATGGAAGAGAATTGATGAAGAGGTAGCTGGTTAAATTACATGCATCTGTTTTCACTTGATTTTAAGTTTTTATGCTCCACAATTATCTGGTCAAGAACATTGAATATATATGTTCTTTACTACAATTGTGATTTCTAACTAATTTATTTTTGCTATATTAGATCGAAGCAACTGTCATGCCTGATGATTATCGGAATAGAAAGGTAAAACTATAGTCCTAGTAATACTAGTTTAATACATGCTATCTCTAATTGTCTACATTTCATTCACAGCCGCAAGAACCTTCATATTTGACATCAAACATGCTTATGGGCATGACCAATGTTGTCAATTGTGCGTTGCAGAAAATAGAGATTCGTTAAAATTTCGATACGCTACAGTGCTATAGCGTCGCTATAGCCCACTATTTGACTTCACTTTGTACTAAATAGCGTATTGCAAACGATAGTGATTTGTTCAAATTCTGTTTTGCTATAACTGTTATTTGACAATACTAAGCATGAATGGACCTTTTGTCCTTTCTTTCACATGCATATATCATGGTTATATTAGCAAATTTCTGAGAACTAAAACAGCACCGTGTTGCAGGTTTGGATACTTTGTAACGACTGCAACGATACAACCGAAGTTTCCTTCCACATTATTGGGCATAAATGCGGTCACTGCAGTTCATATAACACTCGTGCTATTGCTCCTCCTGTTCTTCCGCAATAA
Protein sequence

>MS.gene00991.t1

MEASAIERLDFGKMGYGCKHYRRRCRIRAPCCNEVYSCRHCHNEATSMLKNPFDHHELVRNDVEQVVCSVCDTEQPVAQVCTNCGVRMGEYYCDLCKFFDDDTGKQQFHCDDCGICRVGGSENYFHCKKCGSCYSVALRDNHLCVENSMRHHCPICYEYLFDSLKDTTVMKCGHTMHCECYHEMIKRDKYCCPICSKSVIDMSTAWKRIDEEIEATVMPDDYRNRKVWILCNDCNDTTEVSFHIIGHKCGHCSSYNTRAIAPPVLPQ