Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017522.t1 | XP_003629130.1 | 96.2 | 260 | 9 | 1 | 1 | 260 | 32 | 290 | 2.00E-137 | 498.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017522.t1 | Q5E9J1 | 34.5 | 203 | 106 | 4 | 55 | 252 | 12 | 192 | 1.2e-26 | 121.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017522.t1 | G7L9D8 | 96.2 | 260 | 9 | 1 | 1 | 260 | 32 | 290 | 1.4e-137 | 498.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene31434 | MS.gene017522 | PPI |
| MS.gene017522 | MS.gene75015 | PPI |
| MS.gene029818 | MS.gene017522 | PPI |
| MS.gene055908 | MS.gene017522 | PPI |
| MS.gene028868 | MS.gene017522 | PPI |
| MS.gene043455 | MS.gene017522 | PPI |
| MS.gene024268 | MS.gene017522 | PPI |
| MS.gene54953 | MS.gene017522 | PPI |
| MS.gene065379 | MS.gene017522 | PPI |
| MS.gene055778 | MS.gene017522 | PPI |
| MS.gene24612 | MS.gene017522 | PPI |
| MS.gene033319 | MS.gene017522 | PPI |
| MS.gene011357 | MS.gene017522 | PPI |
| MS.gene48301 | MS.gene017522 | PPI |
| MS.gene033701 | MS.gene017522 | PPI |
| MS.gene059960 | MS.gene017522 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017522.t1 | MTR_8g073580 | 98.846 | 260 | 2 | 1 | 1 | 260 | 32 | 290 | 0.0 | 521 |
| MS.gene017522.t1 | MTR_8g073580 | 99.138 | 232 | 1 | 1 | 29 | 260 | 13 | 243 | 1.87e-169 | 468 |
| MS.gene017522.t1 | MTR_3g061590 | 52.124 | 259 | 105 | 6 | 9 | 259 | 12 | 259 | 2.45e-77 | 235 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017522.t1 | AT5G66010 | 69.962 | 263 | 61 | 4 | 1 | 260 | 8 | 255 | 2.68e-121 | 346 |
| MS.gene017522.t1 | AT5G66010 | 70.186 | 161 | 42 | 2 | 103 | 260 | 1 | 158 | 6.75e-71 | 215 |
| MS.gene017522.t1 | AT3G20890 | 42.955 | 291 | 129 | 6 | 3 | 259 | 5 | 292 | 9.77e-66 | 206 |
| MS.gene017522.t1 | AT3G20890 | 42.365 | 203 | 87 | 3 | 87 | 259 | 27 | 229 | 2.37e-46 | 155 |
Find 0 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTACAAGCTAATAGAAGAT+AGG | + | 57641:64639-64658 | MS.gene017522:CDS | 25.0% |
| ! | TTACAAGCTAATAGAAGATA+GGG | + | 57641:64640-64659 | MS.gene017522:CDS | 25.0% |
| ATAAAATGACCATTGGATCA+AGG | + | 57641:64756-64775 | MS.gene017522:CDS | 30.0% | |
| ATATATGACAACGATTACCA+AGG | + | 57641:64491-64510 | MS.gene017522:CDS | 30.0% | |
| TATCAACATTATGGATATGC+CGG | + | 57641:64167-64186 | MS.gene017522:CDS | 30.0% | |
| TTCAAGGATAAAATGACCAT+TGG | + | 57641:64749-64768 | MS.gene017522:CDS | 30.0% | |
| CTCAAAGAGACAAAGAATGA+TGG | + | 57641:64103-64122 | MS.gene017522:CDS | 35.0% | |
| GATTATTACAATGCTGTTGC+TGG | + | 57641:64449-64468 | MS.gene017522:CDS | 35.0% | |
| GTTCAGCGATAAAGAACAAA+TGG | + | 57641:64541-64560 | MS.gene017522:CDS | 35.0% | |
| TCTATGTCTGTGCAGTTAAA+CGG | - | 57641:64248-64267 | None:intergenic | 35.0% | |
| TGAGATTTAGTCACCAAGAA+AGG | - | 57641:64599-64618 | None:intergenic | 35.0% | |
| !!! | GACATTTTGAAGTTCTTTGC+TGG | + | 57641:64266-64285 | MS.gene017522:CDS | 35.0% |
| ACAGGCAGCTATCAACATTA+TGG | + | 57641:64158-64177 | MS.gene017522:CDS | 40.0% | |
| ACTGAGATATTGAAGATGCG+TGG | + | 57641:64569-64588 | MS.gene017522:CDS | 40.0% | |
| TACACCAGATGAAGCTAGAA+GGG | + | 57641:64799-64818 | MS.gene017522:CDS | 40.0% | |
| TAGCTTCATCTGGTGTAGAA+GGG | - | 57641:64796-64815 | None:intergenic | 40.0% | |
| TTCCACATACCTTGATCCAA+TGG | - | 57641:64768-64787 | None:intergenic | 40.0% | |
| TTCTTTATCGCTGAACCTCT+TGG | - | 57641:64537-64556 | None:intergenic | 40.0% | |
| ! | GCAGATATGTGGAAGTTTTC+AGG | + | 57641:64414-64433 | MS.gene017522:CDS | 40.0% |
| ! | TTTTCCTGTGGTTCGTCTAA+GGG | + | 57641:64217-64236 | MS.gene017522:CDS | 40.0% |
| !!! | AGTTTTCAGGTGTAAGAAGC+AGG | + | 57641:64427-64446 | MS.gene017522:CDS | 40.0% |
| ACAGAGAGATCGACAGAACA+TGG | + | 57641:64388-64407 | MS.gene017522:CDS | 45.0% | |
| AGATGGTAAAGCTACAGGAG+AGG | + | 57641:64682-64701 | MS.gene017522:CDS | 45.0% | |
| ATTGCTCTCTTTGCCTCATC+TGG | - | 57641:64728-64747 | None:intergenic | 45.0% | |
| CAACATTATGGATATGCCGG+TGG | + | 57641:64170-64189 | MS.gene017522:CDS | 45.0% | |
| CAGAGAGATCGACAGAACAT+GGG | + | 57641:64389-64408 | MS.gene017522:CDS | 45.0% | |
| CTACACCAGATGAAGCTAGA+AGG | + | 57641:64798-64817 | MS.gene017522:CDS | 45.0% | |
| CTAGCTTCATCTGGTGTAGA+AGG | - | 57641:64797-64816 | None:intergenic | 45.0% | |
| CTTAGACGAACCACAGGAAA+AGG | - | 57641:64218-64237 | None:intergenic | 45.0% | |
| GACCAGTAACACATCGACAA+TGG | - | 57641:64297-64316 | None:intergenic | 45.0% | |
| GACCATTGGATCAAGGTATG+TGG | + | 57641:64763-64782 | MS.gene017522:CDS | 45.0% | |
| GACCATTGTCGATGTGTTAC+TGG | + | 57641:64292-64311 | MS.gene017522:CDS | 45.0% | |
| GCTGGTGAGATCAATTACGA+AGG | + | 57641:64467-64486 | MS.gene017522:CDS | 45.0% | |
| GGCAAAGAGAGCAATGTTCA+AGG | + | 57641:64733-64752 | MS.gene017522:CDS | 45.0% | |
| TACTGGTCAACAAGAGTGGA+CGG | + | 57641:64309-64328 | MS.gene017522:CDS | 45.0% | |
| TCACTGCCTTGATCTTGACT+CGG | - | 57641:64825-64844 | None:intergenic | 45.0% | |
| TGCTCCTGCAAAGACTACAA+AGG | - | 57641:64348-64367 | None:intergenic | 45.0% | |
| TTTCCTGTGGTTCGTCTAAG+GGG | + | 57641:64218-64237 | MS.gene017522:CDS | 45.0% | |
| ! | AGAGTTTGTTTCGCCAGATG+AGG | + | 57641:64712-64731 | MS.gene017522:CDS | 45.0% |
| ! | CTCCTGTAGCTTTACCATCT+GGG | - | 57641:64682-64701 | None:intergenic | 45.0% |
| ! | CTTTTCCTGTGGTTCGTCTA+AGG | + | 57641:64216-64235 | MS.gene017522:CDS | 45.0% |
| ! | GTGTTACTGGTCAACAAGAG+TGG | + | 57641:64305-64324 | MS.gene017522:CDS | 45.0% |
| ! | TCTCCTGTAGCTTTACCATC+TGG | - | 57641:64683-64702 | None:intergenic | 45.0% |
| AACAAGAGTGGACGGTTCTC+TGG | + | 57641:64317-64336 | MS.gene017522:CDS | 50.0% | |
| AACCACAGGAAAAGGAGGAG+GGG | - | 57641:64210-64229 | None:intergenic | 50.0% | |
| AGACGAACCACAGGAAAAGG+AGG | - | 57641:64215-64234 | None:intergenic | 50.0% | |
| AGTCTTTGCAGGAGCAATGC+AGG | + | 57641:64352-64371 | MS.gene017522:CDS | 50.0% | |
| CATTATGGATATGCCGGTGG+TGG | + | 57641:64173-64192 | MS.gene017522:CDS | 50.0% | |
| GAACATGGGTCGCAGATATG+TGG | + | 57641:64403-64422 | MS.gene017522:CDS | 50.0% | |
| GAACCACAGGAAAAGGAGGA+GGG | - | 57641:64211-64230 | None:intergenic | 50.0% | |
| GAGGCCTTTGTAGTCTTTGC+AGG | + | 57641:64341-64360 | MS.gene017522:CDS | 50.0% | |
| GATGCGTGGACTTCCTTTCT+TGG | + | 57641:64583-64602 | MS.gene017522:CDS | 50.0% | |
| GCTCACTGCGAAGTATGGAT+TGG | - | 57641:64132-64151 | None:intergenic | 50.0% | |
| TTCCTGTGGTTCGTCTAAGG+GGG | + | 57641:64219-64238 | MS.gene017522:CDS | 50.0% | |
| ACCACAGGAAAAGGAGGAGG+GGG | - | 57641:64209-64228 | None:intergenic | 55.0% | |
| ATCGCTGAACCTCTTGGACC+TGG | - | 57641:64531-64550 | None:intergenic | 55.0% | |
| CACATTGCATGTCGCCCAGA+TGG | + | 57641:64665-64684 | MS.gene017522:CDS | 55.0% | |
| CCACTGCTCACTGCGAAGTA+TGG | - | 57641:64137-64156 | None:intergenic | 55.0% | |
| CCATACTTCGCAGTGAGCAG+TGG | + | 57641:64134-64153 | MS.gene017522:CDS | 55.0% | |
| CGAACCACAGGAAAAGGAGG+AGG | - | 57641:64212-64231 | None:intergenic | 55.0% | |
| CGCCCAGATGGTAAAGCTAC+AGG | + | 57641:64677-64696 | MS.gene017522:CDS | 55.0% | |
| CTGAACCTCTTGGACCTGGA+GGG | - | 57641:64527-64546 | None:intergenic | 55.0% | |
| GAAGGGCCGAGTCAAGATCA+AGG | + | 57641:64816-64835 | MS.gene017522:CDS | 55.0% | |
| TTCGCAGTGAGCAGTGGAAC+AGG | + | 57641:64140-64159 | MS.gene017522:CDS | 55.0% | |
| ! | TATGGATATGCCGGTGGTGG+CGG | + | 57641:64176-64195 | MS.gene017522:CDS | 55.0% |
| !! | TCGGCCCTTCTAGCTTCATC+TGG | - | 57641:64806-64825 | None:intergenic | 55.0% |
| AACCTCTTGGACCTGGAGGG+AGG | - | 57641:64524-64543 | None:intergenic | 60.0% | |
| AGCCCCCTTAGACGAACCAC+AGG | - | 57641:64224-64243 | None:intergenic | 60.0% | |
| GCTGAACCTCTTGGACCTGG+AGG | - | 57641:64528-64547 | None:intergenic | 60.0% | |
| GTTTCGGACGGGTACGAGGT+TGG | + | 57641:64080-64099 | MS.gene017522:CDS | 60.0% | |
| TCTTGGACCTGGAGGGAGGA+GGG | - | 57641:64520-64539 | None:intergenic | 60.0% | |
| ! | GAGTGGACGGTTCTCTGGAG+AGG | + | 57641:64322-64341 | MS.gene017522:CDS | 60.0% |
| AAGGAAGCCCTCCTCCCTCC+AGG | + | 57641:64510-64529 | MS.gene017522:CDS | 65.0% | |
| CAGGAAAAGGAGGAGGGGGC+TGG | - | 57641:64205-64224 | None:intergenic | 65.0% | |
| CTCCTCCCTCCAGGTCCAAG+AGG | + | 57641:64519-64538 | MS.gene017522:CDS | 65.0% | |
| CTCTTGGACCTGGAGGGAGG+AGG | - | 57641:64521-64540 | None:intergenic | 65.0% | |
| GTTGGGAAGCGGGGGGGTTT+CGG | + | 57641:64064-64083 | MS.gene017522:CDS | 65.0% | |
| TGGAGGGAGGAGGGCTTCCT+TGG | - | 57641:64511-64530 | None:intergenic | 65.0% | |
| ! | GCCCCCTCCTCCTTTTCCTG+TGG | + | 57641:64205-64224 | MS.gene017522:CDS | 65.0% |
| GAAGCGGGGGGGTTTCGGAC+GGG | + | 57641:64069-64088 | MS.gene017522:CDS | 70.0% | |
| GGAAGCGGGGGGGTTTCGGA+CGG | + | 57641:64068-64087 | MS.gene017522:CDS | 70.0% | |
| GGGGGTTTCGGACGGGTACG+AGG | + | 57641:64076-64095 | MS.gene017522:CDS | 70.0% | |
| GGGCTGGAAGCCGCCACCAC+CGG | - | 57641:64189-64208 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57641 | gene | 64062 | 64844 | 64062 | ID=MS.gene017522 |
| 57641 | mRNA | 64062 | 64844 | 64062 | ID=MS.gene017522.t1;Parent=MS.gene017522 |
| 57641 | exon | 64062 | 64844 | 64062 | ID=MS.gene017522.t1.exon1;Parent=MS.gene017522.t1 |
| 57641 | CDS | 64062 | 64844 | 64062 | ID=cds.MS.gene017522.t1;Parent=MS.gene017522.t1 |
| Gene Sequence |
| Protein sequence |
