AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene017567

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017567.t1 MTR_8g074510 94.444 288 16 0 1 288 1 288 0.0 551
MS.gene017567.t1 MTR_8g074450 86.552 290 37 1 1 288 1 290 0.0 508
MS.gene017567.t1 MTR_8g074420 82.414 290 49 1 1 288 1 290 6.07e-165 459
MS.gene017567.t1 MTR_5g023550 64.706 289 94 3 1 287 1 283 1.02e-125 360
MS.gene017567.t1 MTR_5g023320 66.899 287 83 5 5 288 6 283 1.36e-125 359
MS.gene017567.t1 MTR_5g023520 63.448 290 99 2 1 288 1 285 5.22e-125 358
MS.gene017567.t1 MTR_5g023600 65.734 286 90 3 4 287 5 284 6.51e-125 358
MS.gene017567.t1 MTR_5g023370 65.263 285 91 3 5 287 6 284 2.53e-124 356
MS.gene017567.t1 MTR_5g023390 64.912 285 92 3 5 287 6 284 4.27e-124 356
MS.gene017567.t1 MTR_5g023340 64.561 285 93 3 5 287 6 284 6.87e-123 353
MS.gene017567.t1 MTR_5g023350 64.336 286 94 3 4 287 5 284 2.14e-122 351
MS.gene017567.t1 MTR_5g023530 63.066 287 97 4 4 287 5 285 7.12e-120 345
MS.gene017567.t1 MTR_5g023470 62.369 287 99 4 4 287 5 285 5.21e-119 343
MS.gene017567.t1 MTR_5g023580 63.251 283 100 2 5 285 6 286 5.13e-115 335
MS.gene017567.t1 MTR_5g023460 61.979 192 63 3 97 288 11 192 4.73e-75 228
MS.gene017567.t1 MTR_8g074530 45.139 288 152 4 4 287 19 304 3.16e-73 227
MS.gene017567.t1 MTR_5g023290 39.667 300 167 4 2 287 9 308 2.59e-63 202
MS.gene017567.t1 MTR_4g070530 33.910 289 178 5 6 287 13 295 2.84e-44 152
MS.gene017567.t1 MTR_5g023650 32.534 292 183 4 8 287 15 304 8.23e-41 143
MS.gene017567.t1 MTR_8g074380 31.488 289 166 5 8 286 15 281 2.18e-38 138
MS.gene017567.t1 MTR_4g070570 29.412 289 197 3 5 287 15 302 2.20e-38 137
MS.gene017567.t1 MTR_3g116950 34.694 294 171 10 5 287 12 295 1.39e-37 135
MS.gene017567.t1 MTR_5g023410 66.000 100 32 1 1 98 1 100 1.46e-37 132
MS.gene017567.t1 MTR_3g116920 32.990 291 182 8 5 287 12 297 1.52e-37 135
MS.gene017567.t1 MTR_4g070540 31.944 288 187 5 6 288 13 296 6.86e-37 133
MS.gene017567.t1 MTR_4g070590 33.106 293 179 7 6 287 14 300 3.27e-35 129
MS.gene017567.t1 MTR_3g115150 27.597 308 183 10 3 287 25 315 2.22e-23 97.8
MS.gene017567.t1 MTR_1g037820 29.046 241 145 5 49 287 9 225 1.43e-22 93.6
MS.gene017567.t1 MTR_5g023540 46.154 65 31 1 163 227 4 64 8.13e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017567.t1 AT4G35690 35.570 298 163 6 1 287 4 283 6.50e-52 172
MS.gene017567.t1 AT2G17680 37.785 307 154 8 1 288 4 292 1.08e-45 156
MS.gene017567.t1 AT4G35710 37.838 296 158 9 1 287 4 282 8.98e-45 153
MS.gene017567.t1 AT2G17080 36.806 288 153 8 1 286 1 261 3.34e-42 146
MS.gene017567.t1 AT4G35200 37.193 285 147 10 1 285 1 253 9.25e-39 137
MS.gene017567.t1 AT2G17070 37.063 286 151 9 1 284 1 259 2.38e-38 136
MS.gene017567.t1 AT4G35210 34.386 285 152 9 1 285 1 250 1.37e-34 125
MS.gene017567.t1 AT4G35660 31.834 289 172 8 7 287 16 287 6.57e-28 109
MS.gene017567.t1 AT4G35720 31.746 315 179 10 5 287 14 324 2.36e-25 103
MS.gene017567.t1 AT3G51400 31.399 293 176 10 1 287 1 274 2.18e-21 91.7
MS.gene017567.t1 AT1G76240 27.211 294 194 6 3 288 27 308 2.65e-20 89.0
MS.gene017567.t1 AT4G35680 28.000 300 187 8 8 287 19 309 1.67e-18 84.3
MS.gene017567.t1 AT1G76210 26.667 225 142 5 63 287 24 225 1.49e-15 74.7
MS.gene017567.t1 AT3G51410 29.893 281 155 6 8 287 40 279 6.39e-15 73.6

Find 43 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGCATGTCTTCAATTT+TGG 0.093147 8.1:-32348458 MS.gene017567:CDS
TTCACTTAAAAGAGTGTTTA+AGG 0.140662 8.1:+32348219 None:intergenic
AAGCAAATGGATAGTAAATT+TGG 0.146612 8.1:-32348475 MS.gene017567:CDS
AGATAGTTCAATGAGAATTT+TGG 0.199075 8.1:-32348671 MS.gene017567:CDS
ATGATTTGGAGAACTTATTT+AGG 0.260717 8.1:-32348120 MS.gene017567:CDS
TGAAACTATTGAGAATGATT+TGG 0.295462 8.1:-32348134 MS.gene017567:CDS
AATATATAAATCATCTATTA+AGG 0.302191 8.1:+32348777 None:intergenic
CAATGCATTTCTCACCTCTA+TGG 0.309012 8.1:+32348704 None:intergenic
AAGCCCTTCACTCTTGTCTA+AGG 0.365459 8.1:-32348597 MS.gene017567:CDS
AGATGATTTATATATTTCAT+TGG 0.368321 8.1:-32348770 MS.gene017567:CDS
ACACTCTAATCAAAGAATAA+AGG 0.396201 8.1:+32348422 None:intergenic
ACATCATGCTCCATTCATAT+TGG 0.455596 8.1:-32348805 MS.gene017567:CDS
TGGATATTTGTGGCATCACT+AGG 0.456088 8.1:-32348651 MS.gene017567:CDS
AGATCTAGAAGGCAAACTAA+TGG 0.456112 8.1:+32348891 None:intergenic
GATCGCATCTTTGAAGCAAA+TGG 0.471046 8.1:-32348488 MS.gene017567:CDS
ATGGTGAGAAATCACTTGTT+GGG 0.479630 8.1:+32348723 None:intergenic
TATGGTGAGAAATCACTTGT+TGG 0.484011 8.1:+32348722 None:intergenic
TAAGGAAAGACCAATATGAA+TGG 0.496985 8.1:+32348795 None:intergenic
GGATACAATTCTTCAAATCA+AGG 0.500801 8.1:-32348629 MS.gene017567:CDS
TCTTGTCTAAGGAGAAGAAA+AGG 0.505857 8.1:-32348586 MS.gene017567:CDS
ACATGAAAGATTAGAGACTT+TGG 0.511627 8.1:-32348167 MS.gene017567:CDS
GTGCTAGGATGAGATCTAGA+AGG 0.513144 8.1:+32348880 None:intergenic
TTCTCCTTAGACAAGAGTGA+AGG 0.531189 8.1:+32348593 None:intergenic
TCATCTACTCTAATTGTGCT+AGG 0.541777 8.1:+32348865 None:intergenic
ACCATGGCAATCAAGTATCA+TGG 0.555053 8.1:-32348919 MS.gene017567:CDS
TCTCCTTAGACAAGAGTGAA+GGG 0.564139 8.1:+32348594 None:intergenic
ATTGAGAAATTATATTCAAG+AGG 0.564623 8.1:+32348074 None:intergenic
GGATATTTGTGGCATCACTA+GGG 0.577026 8.1:-32348650 MS.gene017567:CDS
AACAAGCTCAAGACATGGGA+AGG 0.580057 8.1:-32348835 MS.gene017567:CDS
AGGTGAGAAATGCATTGAAG+AGG 0.582191 8.1:-32348698 MS.gene017567:CDS
TTGAGAAATTATATTCAAGA+GGG 0.584586 8.1:+32348075 None:intergenic
TAATCAAAGAATAAAGGTGA+TGG 0.595163 8.1:+32348428 None:intergenic
TAGCACAATTAGAGTAGATG+AGG 0.604276 8.1:-32348863 MS.gene017567:CDS
TTTAGCAACAAATTTCCACT+TGG 0.605269 8.1:+32348315 None:intergenic
TACTAATGGAGAAAACATGC+AGG 0.606065 8.1:-32348194 MS.gene017567:CDS
ATTGGATGATCTTCTCAACA+TGG 0.611714 8.1:-32348752 MS.gene017567:CDS
AATTGAACAAGCTCAAGACA+TGG 0.618716 8.1:-32348840 MS.gene017567:CDS
CAAGTGATTTCTCACCATAG+AGG 0.629127 8.1:-32348718 MS.gene017567:CDS
ATTGAACAAGCTCAAGACAT+GGG 0.630590 8.1:-32348839 MS.gene017567:CDS
GTTGCTAAAATGATGCACAA+AGG 0.639952 8.1:-32348301 MS.gene017567:CDS
TTCAAGTGTTGAAAGAAGTG+TGG 0.668403 8.1:-32348560 MS.gene017567:CDS
CAAAGTCAAAGACAACCAAG+TGG 0.668953 8.1:-32348330 MS.gene017567:CDS
ACCATGATACTTGATTGCCA+TGG 0.702181 8.1:+32348918 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAAATCATCTATTA+AGG + chr8.1:32348214-32348233 None:intergenic 10.0%
!! AGATGATTTATATATTTCAT+TGG - chr8.1:32348218-32348237 MS.gene017567:CDS 15.0%
!! ATTGAGAAATTATATTCAAG+AGG + chr8.1:32348917-32348936 None:intergenic 20.0%
!! TTGAGAAATTATATTCAAGA+GGG + chr8.1:32348916-32348935 None:intergenic 20.0%
! AAGCAAATGGATAGTAAATT+TGG - chr8.1:32348513-32348532 MS.gene017567:CDS 25.0%
! ACACTCTAATCAAAGAATAA+AGG + chr8.1:32348569-32348588 None:intergenic 25.0%
! TAATCAAAGAATAAAGGTGA+TGG + chr8.1:32348563-32348582 None:intergenic 25.0%
! TGAAACTATTGAGAATGATT+TGG - chr8.1:32348854-32348873 MS.gene017567:CDS 25.0%
!! AGATAGTTCAATGAGAATTT+TGG - chr8.1:32348317-32348336 MS.gene017567:CDS 25.0%
!! ATGAGAATTTTGGATATTTG+TGG - chr8.1:32348327-32348346 MS.gene017567:CDS 25.0%
!! ATGATTTGGAGAACTTATTT+AGG - chr8.1:32348868-32348887 MS.gene017567:CDS 25.0%
!!! TTAAACACTCTTTTAAGTGA+AGG - chr8.1:32348771-32348790 MS.gene017567:CDS 25.0%
!!! TTCACTTAAAAGAGTGTTTA+AGG + chr8.1:32348772-32348791 None:intergenic 25.0%
ACATGAAAGATTAGAGACTT+TGG - chr8.1:32348821-32348840 MS.gene017567:CDS 30.0%
GGATACAATTCTTCAAATCA+AGG - chr8.1:32348359-32348378 MS.gene017567:CDS 30.0%
TAAGGAAAGACCAATATGAA+TGG + chr8.1:32348196-32348215 None:intergenic 30.0%
TTTAGCAACAAATTTCCACT+TGG + chr8.1:32348676-32348695 None:intergenic 30.0%
! ATTTGGCATGTCTTCAATTT+TGG - chr8.1:32348530-32348549 MS.gene017567:CDS 30.0%
!!! CTTTTAAGTGAAGGTACTAA+TGG - chr8.1:32348780-32348799 MS.gene017567:CDS 30.0%
AATTGAACAAGCTCAAGACA+TGG - chr8.1:32348148-32348167 MS.gene017567:CDS 35.0%
ACATCATGCTCCATTCATAT+TGG - chr8.1:32348183-32348202 MS.gene017567:CDS 35.0%
AGATCTAGAAGGCAAACTAA+TGG + chr8.1:32348100-32348119 None:intergenic 35.0%
ATGGTGAGAAATCACTTGTT+GGG + chr8.1:32348268-32348287 None:intergenic 35.0%
ATTGAACAAGCTCAAGACAT+GGG - chr8.1:32348149-32348168 MS.gene017567:CDS 35.0%
ATTGGATGATCTTCTCAACA+TGG - chr8.1:32348236-32348255 MS.gene017567:CDS 35.0%
GTTGCTAAAATGATGCACAA+AGG - chr8.1:32348687-32348706 MS.gene017567:CDS 35.0%
TACTAATGGAGAAAACATGC+AGG - chr8.1:32348794-32348813 MS.gene017567:CDS 35.0%
TAGCACAATTAGAGTAGATG+AGG - chr8.1:32348125-32348144 MS.gene017567:CDS 35.0%
TATGGTGAGAAATCACTTGT+TGG + chr8.1:32348269-32348288 None:intergenic 35.0%
TCATCTACTCTAATTGTGCT+AGG + chr8.1:32348126-32348145 None:intergenic 35.0%
TCTTGTCTAAGGAGAAGAAA+AGG - chr8.1:32348402-32348421 MS.gene017567:CDS 35.0%
!! TTCAAGTGTTGAAAGAAGTG+TGG - chr8.1:32348428-32348447 MS.gene017567:CDS 35.0%
AGGTGAGAAATGCATTGAAG+AGG - chr8.1:32348290-32348309 MS.gene017567:CDS 40.0%
CAAAGTCAAAGACAACCAAG+TGG - chr8.1:32348658-32348677 MS.gene017567:CDS 40.0%
CAAGTGATTTCTCACCATAG+AGG - chr8.1:32348270-32348289 MS.gene017567:CDS 40.0%
CAATGCATTTCTCACCTCTA+TGG + chr8.1:32348287-32348306 None:intergenic 40.0%
GGATATTTGTGGCATCACTA+GGG - chr8.1:32348338-32348357 MS.gene017567:CDS 40.0%
TCTCCTTAGACAAGAGTGAA+GGG + chr8.1:32348397-32348416 None:intergenic 40.0%
TGGATATTTGTGGCATCACT+AGG - chr8.1:32348337-32348356 MS.gene017567:CDS 40.0%
TTCTCCTTAGACAAGAGTGA+AGG + chr8.1:32348398-32348417 None:intergenic 40.0%
!! GATCGCATCTTTGAAGCAAA+TGG - chr8.1:32348500-32348519 MS.gene017567:CDS 40.0%
AACAAGCTCAAGACATGGGA+AGG - chr8.1:32348153-32348172 MS.gene017567:CDS 45.0%
AAGCCCTTCACTCTTGTCTA+AGG - chr8.1:32348391-32348410 MS.gene017567:CDS 45.0%
GTGCTAGGATGAGATCTAGA+AGG + chr8.1:32348111-32348130 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 32348072 32348938 32348072 ID=MS.gene017567
chr8.1 mRNA 32348072 32348938 32348072 ID=MS.gene017567.t1;Parent=MS.gene017567
chr8.1 exon 32348072 32348938 32348072 ID=MS.gene017567.t1.exon1;Parent=MS.gene017567.t1
chr8.1 CDS 32348072 32348938 32348072 ID=cds.MS.gene017567.t1;Parent=MS.gene017567.t1
Gene Sequence

>MS.gene017567

ATGGCAATCAAGTATCATGGTCGTTCCATTAGTTTGCCTTCTAGATCTCATCCTAGCACAATTAGAGTAGATGAGGAATTGAACAAGCTCAAGACATGGGAAGGAACATCTACATCATGCTCCATTCATATTGGTCTTTCCTTAATAGATGATTTATATATTTCATTGGATGATCTTCTCAACATGGCATCAACCCAACAAGTGATTTCTCACCATAGAGGTGAGAAATGCATTGAAGAGGTGTTAGATAGTTCAATGAGAATTTTGGATATTTGTGGCATCACTAGGGATACAATTCTTCAAATCAAGGAAAATGTTCAAGCCCTTCACTCTTGTCTAAGGAGAAGAAAAGGTGATTCAAGTGTTGAAAGAAGTGTGGTAGAATACAAATTCTTCACAAAAAAGATGAAAAAAAATGTCAACAAGTTGATCGCATCTTTGAAGCAAATGGATAGTAAATTTGGCATGTCTTCAATTTTGGAACTTGACCATCACCTTTATTCTTTGATTAGAGTGTTTAAAGAAGTCATTGCAATGAATTTGTCTAATTTTCAAATCATTTTGTCATTCTTGACTATGTCTTCATCAAAGTCAAAGACAACCAAGTGGAAATTTGTTGCTAAAATGATGCACAAAGGAGTCATAGCATGTGAAGATAACTCAGATAATGTCAATGAGTTTTTGTGTGTTGAAGCTACCTTAAACACTCTTTTAAGTGAAGGTACTAATGGAGAAAACATGCAGGCTGCACATGAAAGATTAGAGACTTTGGAGAATGCAATTGAAACTATTGAGAATGATTTGGAGAACTTATTTAGGCGCTTAATAAAATCTAGAGCTTCCCTCTTGAATATAATTTCTCAATAG
Protein sequence

>MS.gene017567.t1

MAIKYHGRSISLPSRSHPSTIRVDEELNKLKTWEGTSTSCSIHIGLSLIDDLYISLDDLLNMASTQQVISHHRGEKCIEEVLDSSMRILDICGITRDTILQIKENVQALHSCLRRRKGDSSVERSVVEYKFFTKKMKKNVNKLIASLKQMDSKFGMSSILELDHHLYSLIRVFKEVIAMNLSNFQIILSFLTMSSSKSKTTKWKFVAKMMHKGVIACEDNSDNVNEFLCVEATLNTLLSEGTNGENMQAAHERLETLENAIETIENDLENLFRRLIKSRASLLNIISQ