AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67587

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67587.t1 MTR_5g023550 90.909 275 25 0 1 275 1 275 6.40e-177 489
MS.gene67587.t1 MTR_5g023350 89.781 274 28 0 2 275 3 276 3.64e-175 484
MS.gene67587.t1 MTR_5g023370 89.416 274 29 0 2 275 3 276 4.39e-175 484
MS.gene67587.t1 MTR_5g023390 89.051 274 30 0 2 275 3 276 4.79e-175 484
MS.gene67587.t1 MTR_5g023520 88.406 276 31 1 1 275 1 276 1.27e-173 480
MS.gene67587.t1 MTR_5g023600 89.051 274 30 0 2 275 3 276 7.26e-173 479
MS.gene67587.t1 MTR_5g023530 88.321 274 31 1 3 275 4 277 2.30e-171 475
MS.gene67587.t1 MTR_5g023340 87.591 274 34 0 2 275 3 276 1.97e-170 473
MS.gene67587.t1 MTR_5g023320 87.636 275 30 2 2 275 3 274 1.80e-169 470
MS.gene67587.t1 MTR_5g023470 87.226 274 34 1 3 275 4 277 3.93e-169 469
MS.gene67587.t1 MTR_5g023580 82.374 278 45 2 2 275 3 280 1.51e-154 435
MS.gene67587.t1 MTR_8g074450 68.327 281 83 2 1 275 1 281 1.37e-135 384
MS.gene67587.t1 MTR_8g074510 66.548 281 86 3 1 275 1 279 3.04e-133 378
MS.gene67587.t1 MTR_8g074420 67.260 281 86 2 1 275 1 281 6.59e-129 367
MS.gene67587.t1 MTR_5g023460 84.181 177 24 1 99 275 11 183 8.24e-106 305
MS.gene67587.t1 MTR_8g074530 44.326 282 147 5 2 275 17 296 2.25e-66 209
MS.gene67587.t1 MTR_5g023290 36.986 292 166 5 2 275 9 300 8.77e-57 184
MS.gene67587.t1 MTR_5g023410 90.000 100 10 0 1 100 1 100 7.37e-53 171
MS.gene67587.t1 MTR_5g023650 36.458 288 167 4 3 275 10 296 1.51e-47 160
MS.gene67587.t1 MTR_8g074380 36.042 283 155 6 2 275 9 274 5.50e-46 157
MS.gene67587.t1 MTR_4g070530 33.696 276 176 4 6 275 13 287 9.19e-44 150
MS.gene67587.t1 MTR_4g070540 32.975 279 174 5 6 275 13 287 5.23e-39 138
MS.gene67587.t1 MTR_3g116950 35.211 284 167 10 3 275 10 287 5.28e-39 138
MS.gene67587.t1 MTR_4g070590 34.875 281 170 7 6 275 14 292 2.22e-36 131
MS.gene67587.t1 MTR_4g070570 30.903 288 181 9 2 275 11 294 5.82e-36 130
MS.gene67587.t1 MTR_3g116920 34.266 286 169 10 3 275 10 289 2.48e-35 129
MS.gene67587.t1 MTR_3g115150 28.328 293 176 9 5 275 27 307 2.98e-23 97.1
MS.gene67587.t1 MTR_1g037820 30.531 226 139 5 51 275 9 217 3.45e-23 95.1
MS.gene67587.t1 MTR_5g023540 54.412 68 28 1 162 229 1 65 6.49e-17 73.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67587.t1 AT4G35690 38.434 281 158 7 1 275 4 275 2.06e-53 175
MS.gene67587.t1 AT2G17680 38.908 293 148 10 1 275 4 283 1.32e-45 155
MS.gene67587.t1 AT2G17080 36.232 276 153 6 1 275 1 254 1.05e-44 152
MS.gene67587.t1 AT4G35710 37.063 286 154 7 1 275 4 274 2.32e-41 144
MS.gene67587.t1 AT2G17070 35.145 276 156 6 1 275 1 254 2.62e-41 143
MS.gene67587.t1 AT4G35200 33.696 276 153 7 1 275 1 247 1.19e-40 141
MS.gene67587.t1 AT4G35210 32.246 276 154 7 1 275 1 244 3.39e-37 132
MS.gene67587.t1 AT4G35720 33.632 223 133 6 5 216 14 232 1.04e-24 101
MS.gene67587.t1 AT4G35660 29.795 292 162 9 3 275 12 279 8.68e-22 92.4
MS.gene67587.t1 AT4G35680 35.354 198 116 4 3 193 14 206 1.16e-21 92.8
MS.gene67587.t1 AT1G76240 26.316 285 182 7 5 275 29 299 1.21e-18 84.3
MS.gene67587.t1 AT3G51400 30.903 288 164 10 1 275 1 266 1.70e-17 80.5
MS.gene67587.t1 AT1G76210 26.754 228 124 6 61 275 20 217 2.33e-14 70.9
MS.gene67587.t1 AT3G51410 35.075 134 73 3 7 140 39 158 2.51e-12 65.9

Find 46 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATATGGAAAGTAAATT+TGG 0.150071 5.2:+14244472 MS.gene67587:CDS
CATTAGAACCTTCACTTATA+AGG 0.159224 5.2:-14244735 None:intergenic
ATTTGAGCGAACATGGTATT+TGG 0.227902 5.2:-14244032 None:intergenic
GCAATTGAAAGTATAGAAAA+TGG 0.264764 5.2:+14244790 MS.gene67587:CDS
CTTTGTCACTTTCAACCATT+TGG 0.324091 5.2:-14244632 None:intergenic
ATTAGAACCTTCACTTATAA+GGG 0.348108 5.2:-14244734 None:intergenic
AGAGAATATATTTAGGCGTT+TGG 0.352912 5.2:+14244816 MS.gene67587:CDS
TGAAGATTTGTATATTTCAT+TGG 0.354547 5.2:+14244177 MS.gene67587:CDS
GATCACATCTTTAAAACATA+TGG 0.374642 5.2:+14244459 MS.gene67587:CDS
CACTTCACTCCGCTCTTAGA+AGG 0.374965 5.2:+14244353 MS.gene67587:CDS
TGTTCAATTCTAGTGGTGCT+TGG 0.418571 5.2:-14244076 None:intergenic
GAAAATTGTGGAAGAGTTGT+TGG 0.420097 5.2:+14244255 MS.gene67587:CDS
ACACGAAAGATTTGAGGCTT+TGG 0.432633 5.2:+14244762 MS.gene67587:CDS
GGAAGAGTTGTTGGATGGTT+CGG 0.441396 5.2:+14244264 MS.gene67587:CDS
ATGGTTTAGAGAATATATTT+AGG 0.442461 5.2:+14244809 MS.gene67587:CDS
TGTTGCACACGAAAGATTTG+AGG 0.458192 5.2:+14244756 MS.gene67587:CDS
CTCACCTTGATGGTGAGAAA+CGG 0.477848 5.2:-14244233 None:intergenic
CTCCGCTCTTAGAAGGAGAA+AGG 0.483537 5.2:+14244360 MS.gene67587:CDS
ACATATGTCATCAACACAAA+AGG 0.492531 5.2:+14244210 MS.gene67587:CDS
TAACTCTTGTTCAATTCTAG+TGG 0.494436 5.2:-14244083 None:intergenic
ACAAATCTTCAAGAAAGAAA+AGG 0.508287 5.2:-14244165 None:intergenic
TGACAAAGTTGATGCAAAAG+AGG 0.510512 5.2:+14244647 MS.gene67587:CDS
AGATTTGTATATTTCATTGG+AGG 0.519503 5.2:+14244180 MS.gene67587:CDS
TCCGCTCTTAGAAGGAGAAA+GGG 0.536323 5.2:+14244361 MS.gene67587:CDS
TAGATATATGTGGCATCACA+AGG 0.540397 5.2:+14244296 MS.gene67587:CDS
TTGAGAACCCTTATAAGTGA+AGG 0.541465 5.2:+14244727 MS.gene67587:CDS
CCATCAAGGTGAGAAAATTG+TGG 0.548327 5.2:+14244243 MS.gene67587:CDS
TTCAAGAAAGAAAAGGCCAT+TGG 0.559275 5.2:-14244158 None:intergenic
GGTATCATGTGAAGAGAACA+TGG 0.559553 5.2:+14244669 MS.gene67587:CDS
CAAAGTCAAAGGCAACCAAA+TGG 0.562903 5.2:+14244617 MS.gene67587:CDS
GAAAACTATTTGAGCGAACA+TGG 0.573814 5.2:-14244039 None:intergenic
TGGATCTTCATCAAAGTCAA+AGG 0.588491 5.2:+14244606 MS.gene67587:CDS
GACAAAGTTGATGCAAAAGA+GGG 0.591207 5.2:+14244648 MS.gene67587:CDS
TCCCTTTCTCCTTCTAAGAG+CGG 0.591308 5.2:-14244362 None:intergenic
ATTGTGGAAGAGTTGTTGGA+TGG 0.606512 5.2:+14244259 MS.gene67587:CDS
TTTCAATGAATTGCAGTGTG+TGG 0.611573 5.2:+14244696 MS.gene67587:CDS
ATCAGATGTGGATGTTGATG+TGG 0.611639 5.2:-14244128 None:intergenic
AATTATTCACAAAGAAGATG+AGG 0.613338 5.2:+14244419 MS.gene67587:CDS
TGTGATTACAGTTCTTAGAG+AGG 0.616682 5.2:+14244534 MS.gene67587:CDS
ACATCTGATTCAATTACCAA+TGG 0.619749 5.2:+14244142 MS.gene67587:CDS
ATTGGTAATTGAATCAGATG+TGG 0.626315 5.2:-14244140 None:intergenic
AAGGCCGTTTCTCACCATCA+AGG 0.642576 5.2:+14244229 MS.gene67587:CDS
TTGATCTTGATCTAATAGTG+AGG 0.655843 5.2:-14244500 None:intergenic
GAACTGTAATCACAGCAACA+AGG 0.656672 5.2:-14244525 None:intergenic
AGATATATGTGGCATCACAA+GGG 0.670882 5.2:+14244297 MS.gene67587:CDS
GTGCTTGGATGAGATTGAGA+AGG 0.684385 5.2:-14244061 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATATGGAAAGTAAATT+TGG + chr5.2:14244472-14244491 MS.gene67587:CDS 20.0%
!! ATGGTTTAGAGAATATATTT+AGG + chr5.2:14244809-14244828 MS.gene67587:CDS 20.0%
!! TGAAGATTTGTATATTTCAT+TGG + chr5.2:14244177-14244196 MS.gene67587:CDS 20.0%
!!! ATTTTCTTTAATCTGTAACA+TGG - chr5.2:14244326-14244345 None:intergenic 20.0%
! AATTATTCACAAAGAAGATG+AGG + chr5.2:14244419-14244438 MS.gene67587:CDS 25.0%
! ACAAATCTTCAAGAAAGAAA+AGG - chr5.2:14244168-14244187 None:intergenic 25.0%
! AGATTTGTATATTTCATTGG+AGG + chr5.2:14244180-14244199 MS.gene67587:CDS 25.0%
! AGTTAACAAAAATGAAGACA+TGG + chr5.2:14244101-14244120 MS.gene67587:CDS 25.0%
! ATTAGAACCTTCACTTATAA+GGG - chr5.2:14244737-14244756 None:intergenic 25.0%
! GTTAACAAAAATGAAGACAT+GGG + chr5.2:14244102-14244121 MS.gene67587:CDS 25.0%
!! GCAATTGAAAGTATAGAAAA+TGG + chr5.2:14244790-14244809 MS.gene67587:CDS 25.0%
!! GTGAAACTTTTAGATATATG+TGG + chr5.2:14244286-14244305 MS.gene67587:CDS 25.0%
!!! GATCACATCTTTAAAACATA+TGG + chr5.2:14244459-14244478 MS.gene67587:CDS 25.0%
ACATATGTCATCAACACAAA+AGG + chr5.2:14244210-14244229 MS.gene67587:CDS 30.0%
ACATCTGATTCAATTACCAA+TGG + chr5.2:14244142-14244161 MS.gene67587:CDS 30.0%
AGAGAATATATTTAGGCGTT+TGG + chr5.2:14244816-14244835 MS.gene67587:CDS 30.0%
CATTAGAACCTTCACTTATA+AGG - chr5.2:14244738-14244757 None:intergenic 30.0%
TAACTCTTGTTCAATTCTAG+TGG - chr5.2:14244086-14244105 None:intergenic 30.0%
TTGATCTTGATCTAATAGTG+AGG - chr5.2:14244503-14244522 None:intergenic 30.0%
! ATTGGTAATTGAATCAGATG+TGG - chr5.2:14244143-14244162 None:intergenic 30.0%
! TCAATCTGTTTTGTCTTTCT+TGG + chr5.2:14244582-14244601 MS.gene67587:CDS 30.0%
AGATATATGTGGCATCACAA+GGG + chr5.2:14244297-14244316 MS.gene67587:CDS 35.0%
ATTTGAGCGAACATGGTATT+TGG - chr5.2:14244035-14244054 None:intergenic 35.0%
GAAAACTATTTGAGCGAACA+TGG - chr5.2:14244042-14244061 None:intergenic 35.0%
GAAAATTGTGGAAGAGTTGT+TGG + chr5.2:14244255-14244274 MS.gene67587:CDS 35.0%
TAGATATATGTGGCATCACA+AGG + chr5.2:14244296-14244315 MS.gene67587:CDS 35.0%
TGGATCTTCATCAAAGTCAA+AGG + chr5.2:14244606-14244625 MS.gene67587:CDS 35.0%
TGTGATTACAGTTCTTAGAG+AGG + chr5.2:14244534-14244553 MS.gene67587:CDS 35.0%
TTCAAGAAAGAAAAGGCCAT+TGG - chr5.2:14244161-14244180 None:intergenic 35.0%
TTGAGAACCCTTATAAGTGA+AGG + chr5.2:14244727-14244746 MS.gene67587:CDS 35.0%
TTTCAATGAATTGCAGTGTG+TGG + chr5.2:14244696-14244715 MS.gene67587:CDS 35.0%
! TCTGTTTTGTCTTTCTTGGT+TGG + chr5.2:14244586-14244605 MS.gene67587:CDS 35.0%
!! CTTTGTCACTTTCAACCATT+TGG - chr5.2:14244635-14244654 None:intergenic 35.0%
!! GACAAAGTTGATGCAAAAGA+GGG + chr5.2:14244648-14244667 MS.gene67587:CDS 35.0%
!! TGACAAAGTTGATGCAAAAG+AGG + chr5.2:14244647-14244666 MS.gene67587:CDS 35.0%
ATCAGATGTGGATGTTGATG+TGG - chr5.2:14244131-14244150 None:intergenic 40.0%
ATTGTGGAAGAGTTGTTGGA+TGG + chr5.2:14244259-14244278 MS.gene67587:CDS 40.0%
CAAAGTCAAAGGCAACCAAA+TGG + chr5.2:14244617-14244636 MS.gene67587:CDS 40.0%
CCATCAAGGTGAGAAAATTG+TGG + chr5.2:14244243-14244262 MS.gene67587:CDS 40.0%
GAACTGTAATCACAGCAACA+AGG - chr5.2:14244528-14244547 None:intergenic 40.0%
GGTATCATGTGAAGAGAACA+TGG + chr5.2:14244669-14244688 MS.gene67587:CDS 40.0%
TGTTCAATTCTAGTGGTGCT+TGG - chr5.2:14244079-14244098 None:intergenic 40.0%
TGTTGCACACGAAAGATTTG+AGG + chr5.2:14244756-14244775 MS.gene67587:CDS 40.0%
! ACACGAAAGATTTGAGGCTT+TGG + chr5.2:14244762-14244781 MS.gene67587:CDS 40.0%
! CCACAATTTTCTCACCTTGA+TGG - chr5.2:14244246-14244265 None:intergenic 40.0%
GGAAGAGTTGTTGGATGGTT+CGG + chr5.2:14244264-14244283 MS.gene67587:CDS 45.0%
GTGCTTGGATGAGATTGAGA+AGG - chr5.2:14244064-14244083 None:intergenic 45.0%
TCCGCTCTTAGAAGGAGAAA+GGG + chr5.2:14244361-14244380 MS.gene67587:CDS 45.0%
! TCCCTTTCTCCTTCTAAGAG+CGG - chr5.2:14244365-14244384 None:intergenic 45.0%
!! CTCACCTTGATGGTGAGAAA+CGG - chr5.2:14244236-14244255 None:intergenic 45.0%
AAGGCCGTTTCTCACCATCA+AGG + chr5.2:14244229-14244248 MS.gene67587:CDS 50.0%
CACTTCACTCCGCTCTTAGA+AGG + chr5.2:14244353-14244372 MS.gene67587:CDS 50.0%
CTCCGCTCTTAGAAGGAGAA+AGG + chr5.2:14244360-14244379 MS.gene67587:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14244025 14244849 14244025 ID=MS.gene67587
chr5.2 mRNA 14244025 14244849 14244025 ID=MS.gene67587.t1;Parent=MS.gene67587
chr5.2 exon 14244025 14244849 14244025 ID=MS.gene67587.t1.exon1;Parent=MS.gene67587.t1
chr5.2 CDS 14244025 14244849 14244025 ID=cds.MS.gene67587.t1;Parent=MS.gene67587.t1
Gene Sequence

>MS.gene67587

ATGGCAACCAAATACCATGTTCGCTCAAATAGTTTTCCTTCTCAATCTCATCCAAGCACCACTAGAATTGAACAAGAGTTAACAAAAATGAAGACATGGGAAGCCACATCAACATCCACATCTGATTCAATTACCAATGGCCTTTTCTTTCTTGAAGATTTGTATATTTCATTGGAGGATCTTCTACATATGTCATCAACACAAAAGGCCGTTTCTCACCATCAAGGTGAGAAAATTGTGGAAGAGTTGTTGGATGGTTCGGTGAAACTTTTAGATATATGTGGCATCACAAGGGACACCATGTTACAGATTAAAGAAAATGTTCAATCACTTCACTCCGCTCTTAGAAGGAGAAAGGGAGATTCAAGCATCGAAACAAGTGTAGTTGAATATAAATTATTCACAAAGAAGATGAGGAAGAATGTCGCAAAGTTGATCACATCTTTAAAACATATGGAAAGTAAATTTGGAGCTTCCTCACTATTAGATCAAGATCAAGACCTTGTTGCTGTGATTACAGTTCTTAGAGAGGTCGTTGCAATGAACTTGTCTATCTTTCAATCTGTTTTGTCTTTCTTGGTTGGATCTTCATCAAAGTCAAAGGCAACCAAATGGTTGAAAGTGACAAAGTTGATGCAAAAGAGGGTATCATGTGAAGAGAACATGGAGAATTTCAATGAATTGCAGTGTGTGGAAGCATCTTTGAGAACCCTTATAAGTGAAGGTTCTAATGTTGCACACGAAAGATTTGAGGCTTTGGAGAATGCAATTGAAAGTATAGAAAATGGTTTAGAGAATATATTTAGGCGTTTGGTTAAAACTAGA
Protein sequence

>MS.gene67587.t1

MATKYHVRSNSFPSQSHPSTTRIEQELTKMKTWEATSTSTSDSITNGLFFLEDLYISLEDLLHMSSTQKAVSHHQGEKIVEELLDGSVKLLDICGITRDTMLQIKENVQSLHSALRRRKGDSSIETSVVEYKLFTKKMRKNVAKLITSLKHMESKFGASSLLDQDQDLVAVITVLREVVAMNLSIFQSVLSFLVGSSSKSKATKWLKVTKLMQKRVSCEENMENFNELQCVEASLRTLISEGSNVAHERFEALENAIESIENGLENIFRRLVKTR