AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67585.t1 MTR_5g023350 93.694 222 14 0 1 222 65 286 5.23e-150 418
MS.gene67585.t1 MTR_5g023600 91.892 222 18 0 1 222 65 286 2.67e-148 414
MS.gene67585.t1 MTR_5g023370 92.342 222 17 0 1 222 65 286 3.71e-148 414
MS.gene67585.t1 MTR_5g023390 90.991 222 20 0 1 222 65 286 6.34e-146 408
MS.gene67585.t1 MTR_5g023340 91.441 222 19 0 1 222 65 286 6.48e-146 408
MS.gene67585.t1 MTR_5g023550 90.909 220 20 0 1 220 64 283 3.37e-145 406
MS.gene67585.t1 MTR_5g023520 91.855 221 17 1 1 220 64 284 1.22e-144 405
MS.gene67585.t1 MTR_5g023530 91.031 223 19 1 1 222 65 287 1.08e-143 402
MS.gene67585.t1 MTR_5g023470 90.135 223 21 1 1 222 65 287 3.00e-142 399
MS.gene67585.t1 MTR_5g023320 89.140 221 20 2 1 220 65 282 8.07e-137 385
MS.gene67585.t1 MTR_5g023580 86.937 222 25 2 1 218 65 286 7.91e-133 378
MS.gene67585.t1 MTR_5g023460 86.486 185 21 1 36 220 11 191 1.11e-112 320
MS.gene67585.t1 MTR_8g074420 68.584 226 65 2 1 220 64 289 3.24e-105 305
MS.gene67585.t1 MTR_8g074450 69.027 226 64 2 1 220 64 289 3.55e-104 303
MS.gene67585.t1 MTR_8g074510 67.257 226 68 2 1 220 62 287 1.52e-102 298
MS.gene67585.t1 MTR_8g074530 42.222 225 120 5 4 220 82 304 5.49e-43 147
MS.gene67585.t1 MTR_5g023290 35.897 234 136 4 1 220 75 308 3.29e-40 140
MS.gene67585.t1 MTR_5g023650 37.885 227 130 3 4 220 79 304 7.04e-39 136
MS.gene67585.t1 MTR_8g074380 38.914 221 115 5 4 221 80 283 1.18e-38 136
MS.gene67585.t1 MTR_3g116950 36.444 225 129 8 4 219 75 294 6.40e-33 120
MS.gene67585.t1 MTR_4g070530 33.333 225 145 3 1 220 71 295 1.90e-31 117
MS.gene67585.t1 MTR_3g116920 35.371 229 130 8 4 219 73 296 1.34e-30 114
MS.gene67585.t1 MTR_4g070570 32.609 230 138 8 4 219 75 301 3.66e-30 113
MS.gene67585.t1 MTR_4g070590 34.956 226 134 7 4 219 77 299 4.66e-28 108
MS.gene67585.t1 MTR_4g070540 31.858 226 147 4 1 220 71 295 6.95e-26 102
MS.gene67585.t1 MTR_1g037820 31.818 220 132 5 2 220 23 225 1.57e-24 97.1
MS.gene67585.t1 MTR_5g023540 55.072 69 28 1 99 167 1 66 3.42e-17 73.6
MS.gene67585.t1 MTR_5g023410 89.189 37 4 0 1 37 64 100 1.43e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67585.t1 AT4G35690 34.071 226 135 6 1 220 66 283 3.93e-35 126
MS.gene67585.t1 AT2G17080 35.484 217 117 6 4 219 67 261 1.10e-30 114
MS.gene67585.t1 AT2G17680 35.193 233 129 8 1 220 68 291 4.66e-30 113
MS.gene67585.t1 AT2G17070 34.884 215 117 6 4 217 67 259 1.07e-28 108
MS.gene67585.t1 AT4G35200 33.333 219 119 6 1 218 61 253 5.51e-26 101
MS.gene67585.t1 AT4G35210 31.963 219 119 6 1 218 61 250 2.42e-24 97.1
MS.gene67585.t1 AT4G35710 32.035 231 132 6 1 220 66 282 1.75e-23 95.5
MS.gene67585.t1 AT4G35680 31.602 231 136 8 5 220 86 309 4.26e-21 89.7
MS.gene67585.t1 AT1G76210 28.261 230 122 6 4 220 26 225 2.97e-18 80.5
MS.gene67585.t1 AT4G35660 29.583 240 127 7 1 221 72 288 3.85e-17 78.6
MS.gene67585.t1 AT4G35720 30.830 253 137 6 2 220 76 324 1.81e-16 77.0
MS.gene67585.t1 AT1G20520 26.432 227 130 7 4 220 26 225 1.40e-13 67.8
MS.gene67585.t1 AT3G51400 31.401 207 121 7 27 221 78 275 1.41e-13 68.6

Find 34 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATATGGAAAGTAAATT+TGG 0.150071 5.2:+14227191 MS.gene67585:CDS
GAACATCATGACTCTATCTT+AGG 0.153901 5.2:+14227580 MS.gene67585:CDS
CGTTAGAACCTTCACTTATA+AGG 0.174479 5.2:-14227454 None:intergenic
ATTTGGAGCTTCATCACTTT+TGG 0.230544 5.2:+14227208 MS.gene67585:CDS
GCAATTGAAAGTATAGAAAA+TGG 0.264764 5.2:+14227509 MS.gene67585:CDS
GGATGGTTCAGTGAAAATTT+TGG 0.269131 5.2:+14226995 MS.gene67585:CDS
CTTTGTGAAGAATTTATATT+CGG 0.312307 5.2:-14227129 None:intergenic
CACTTCACTCTTCTCTTAGA+AGG 0.337508 5.2:+14227072 MS.gene67585:CDS
AGAGAATATATTTAGGTGTT+TGG 0.341972 5.2:+14227535 MS.gene67585:CDS
GTTAGAACCTTCACTTATAA+GGG 0.365747 5.2:-14227453 None:intergenic
GATCACATCTTTAAAACATA+TGG 0.374642 5.2:+14227178 MS.gene67585:CDS
GAAATTTGTTGAAGAGTTGT+TGG 0.385629 5.2:+14226974 MS.gene67585:CDS
CAACAAATTTCTCACCTTGA+CGG 0.400296 5.2:-14226962 None:intergenic
CAAAGTCAAAGGCAACAAAA+TGG 0.408302 5.2:+14227336 MS.gene67585:CDS
ATGGTTTAGAGAATATATTT+AGG 0.411004 5.2:+14227528 MS.gene67585:CDS
ACATGAAAGATTTGAGGCTT+TGG 0.411831 5.2:+14227481 MS.gene67585:CDS
CGTTGCACATGAAAGATTTG+AGG 0.421906 5.2:+14227475 MS.gene67585:CDS
CTCTTCTCTTAGAAGGAGAA+AGG 0.427476 5.2:+14227079 MS.gene67585:CDS
AACATCATGACTCTATCTTA+GGG 0.475458 5.2:+14227581 MS.gene67585:CDS
CAATATGTCATCAACACAAA+AGG 0.479628 5.2:+14226929 None:intergenic
TGACAAAGTTGATGCAAAAG+AGG 0.510512 5.2:+14227366 MS.gene67585:CDS
TTAAGAACCCTTATAAGTGA+AGG 0.516729 5.2:+14227446 MS.gene67585:CDS
TGGATATCTGTGGCATCACA+AGG 0.539204 5.2:+14227015 MS.gene67585:CDS
TCTTCTCTTAGAAGGAGAAA+GGG 0.543836 5.2:+14227080 MS.gene67585:CDS
CTCACCTTGACGGTGAGCAA+TGG 0.559285 5.2:-14226952 None:intergenic
GGTATCATGTGAAGAGAACA+TGG 0.559553 5.2:+14227388 MS.gene67585:CDS
TGGATCTTCATCAAAGTCAA+AGG 0.588491 5.2:+14227325 MS.gene67585:CDS
GACAAAGTTGATGCAAAAGA+GGG 0.591207 5.2:+14227367 MS.gene67585:CDS
TTTGTTGAAGAGTTGTTGGA+TGG 0.600565 5.2:+14226978 MS.gene67585:CDS
AAGGCCATTGCTCACCGTCA+AGG 0.611754 5.2:+14226948 MS.gene67585:CDS
AGTGATTACAGTTCTTAGAG+AGG 0.623623 5.2:+14227253 MS.gene67585:CDS
CTTCAATGAACTGCAGTGTG+TGG 0.642952 5.2:+14227415 MS.gene67585:CDS
GAACTGTAATCACTGCAACA+AGG 0.656672 5.2:-14227244 None:intergenic
GGATATCTGTGGCATCACAA+GGG 0.673626 5.2:+14227016 MS.gene67585:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCTTTAATTTGTAACA+TGG - chr5.2:14227045-14227064 None:intergenic 15.0%
!! AAACATATGGAAAGTAAATT+TGG + chr5.2:14227191-14227210 MS.gene67585:CDS 20.0%
!! ATGGTTTAGAGAATATATTT+AGG + chr5.2:14227528-14227547 MS.gene67585:CDS 20.0%
!! CTTTGTGAAGAATTTATATT+CGG - chr5.2:14227132-14227151 None:intergenic 20.0%
! AGAGAATATATTTAGGTGTT+TGG + chr5.2:14227535-14227554 MS.gene67585:CDS 25.0%
!! GCAATTGAAAGTATAGAAAA+TGG + chr5.2:14227509-14227528 MS.gene67585:CDS 25.0%
!!! GATCACATCTTTAAAACATA+TGG + chr5.2:14227178-14227197 MS.gene67585:CDS 25.0%
GTTAGAACCTTCACTTATAA+GGG - chr5.2:14227456-14227475 None:intergenic 30.0%
TTAAGAACCCTTATAAGTGA+AGG + chr5.2:14227446-14227465 MS.gene67585:CDS 30.0%
! GTGAAAATTTTGGATATCTG+TGG + chr5.2:14227005-14227024 MS.gene67585:CDS 30.0%
!! GAAATTTGTTGAAGAGTTGT+TGG + chr5.2:14226974-14226993 MS.gene67585:CDS 30.0%
!! TCAATCAGTTTTGTCTTTCT+TGG + chr5.2:14227301-14227320 MS.gene67585:CDS 30.0%
AGTGATTACAGTTCTTAGAG+AGG + chr5.2:14227253-14227272 MS.gene67585:CDS 35.0%
CAAAGTCAAAGGCAACAAAA+TGG + chr5.2:14227336-14227355 MS.gene67585:CDS 35.0%
CAACAAATTTCTCACCTTGA+CGG - chr5.2:14226965-14226984 None:intergenic 35.0%
CGTTAGAACCTTCACTTATA+AGG - chr5.2:14227457-14227476 None:intergenic 35.0%
TCTTCTCTTAGAAGGAGAAA+GGG + chr5.2:14227080-14227099 MS.gene67585:CDS 35.0%
TGGATCTTCATCAAAGTCAA+AGG + chr5.2:14227325-14227344 MS.gene67585:CDS 35.0%
TTTGTTGAAGAGTTGTTGGA+TGG + chr5.2:14226978-14226997 MS.gene67585:CDS 35.0%
! ACATGAAAGATTTGAGGCTT+TGG + chr5.2:14227481-14227500 MS.gene67585:CDS 35.0%
! GGATGGTTCAGTGAAAATTT+TGG + chr5.2:14226995-14227014 MS.gene67585:CDS 35.0%
!! ATTTGGAGCTTCATCACTTT+TGG + chr5.2:14227208-14227227 MS.gene67585:CDS 35.0%
!! GACAAAGTTGATGCAAAAGA+GGG + chr5.2:14227367-14227386 MS.gene67585:CDS 35.0%
!! TCAGTTTTGTCTTTCTTGGT+TGG + chr5.2:14227305-14227324 MS.gene67585:CDS 35.0%
!! TGACAAAGTTGATGCAAAAG+AGG + chr5.2:14227366-14227385 MS.gene67585:CDS 35.0%
CACTTCACTCTTCTCTTAGA+AGG + chr5.2:14227072-14227091 MS.gene67585:CDS 40.0%
CGTTGCACATGAAAGATTTG+AGG + chr5.2:14227475-14227494 MS.gene67585:CDS 40.0%
CTCTTCTCTTAGAAGGAGAA+AGG + chr5.2:14227079-14227098 MS.gene67585:CDS 40.0%
GAACTGTAATCACTGCAACA+AGG - chr5.2:14227247-14227266 None:intergenic 40.0%
GGTATCATGTGAAGAGAACA+TGG + chr5.2:14227388-14227407 MS.gene67585:CDS 40.0%
CTTCAATGAACTGCAGTGTG+TGG + chr5.2:14227415-14227434 MS.gene67585:CDS 45.0%
GGATATCTGTGGCATCACAA+GGG + chr5.2:14227016-14227035 MS.gene67585:CDS 45.0%
TGGATATCTGTGGCATCACA+AGG + chr5.2:14227015-14227034 MS.gene67585:CDS 45.0%
AAGGCCATTGCTCACCGTCA+AGG + chr5.2:14226948-14226967 MS.gene67585:CDS 55.0%
CTCACCTTGACGGTGAGCAA+TGG - chr5.2:14226955-14226974 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14226933 14227601 14226933 ID=MS.gene67585
chr5.2 mRNA 14226933 14227601 14226933 ID=MS.gene67585.t1;Parent=MS.gene67585
chr5.2 exon 14226933 14227601 14226933 ID=MS.gene67585.t1.exon1;Parent=MS.gene67585.t1
chr5.2 CDS 14226933 14227601 14226933 ID=cds.MS.gene67585.t1;Parent=MS.gene67585.t1
Gene Sequence

>MS.gene67585

ATGTCATCAACACAAAAGGCCATTGCTCACCGTCAAGGTGAGAAATTTGTTGAAGAGTTGTTGGATGGTTCAGTGAAAATTTTGGATATCTGTGGCATCACAAGGGATACCATGTTACAAATTAAAGAAAATGTTCAATCACTTCACTCTTCTCTTAGAAGGAGAAAGGGAGATTCAAGCATTGAAAAAAGTGTAGCCGAATATAAATTCTTCACAAAGAAGATGAAGAAAAATGTCACAAAGTTGATCACATCTTTAAAACATATGGAAAGTAAATTTGGAGCTTCATCACTTTTGGATCAAGATCAAGACCTTGTTGCAGTGATTACAGTTCTTAGAGAGGTCATTAAAATGAACTTGTCTATCTTTCAATCAGTTTTGTCTTTCTTGGTTGGATCTTCATCAAAGTCAAAGGCAACAAAATGGTTGAAAGTGACAAAGTTGATGCAAAAGAGGGTATCATGTGAAGAGAACATGGAGAACTTCAATGAACTGCAGTGTGTGGAAGCATCTTTAAGAACCCTTATAAGTGAAGGTTCTAACGTTGCACATGAAAGATTTGAGGCTTTGGAGAATGCAATTGAAAGTATAGAAAATGGTTTAGAGAATATATTTAGGTGTTTGGTTAAAACTAGAGTCTGTCTTTTGAACATCATGACTCTATCTTAG
Protein sequence

>MS.gene67585.t1

MSSTQKAIAHRQGEKFVEELLDGSVKILDICGITRDTMLQIKENVQSLHSSLRRRKGDSSIEKSVAEYKFFTKKMKKNVTKLITSLKHMESKFGASSLLDQDQDLVAVITVLREVIKMNLSIFQSVLSFLVGSSSKSKATKWLKVTKLMQKRVSCEENMENFNELQCVEASLRTLISEGSNVAHERFEALENAIESIENGLENIFRCLVKTRVCLLNIMTLS