AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67584

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67584.t1 MTR_5g023370 92.657 286 21 0 1 286 1 286 0.0 520
MS.gene67584.t1 MTR_5g023390 92.657 286 21 0 1 286 1 286 0.0 520
MS.gene67584.t1 MTR_5g023600 93.357 286 19 0 1 286 1 286 0.0 517
MS.gene67584.t1 MTR_5g023350 93.007 286 20 0 1 286 1 286 0.0 517
MS.gene67584.t1 MTR_5g023530 90.592 287 26 1 1 286 1 287 0.0 506
MS.gene67584.t1 MTR_5g023340 89.860 286 29 0 1 286 1 286 0.0 503
MS.gene67584.t1 MTR_5g023470 89.547 287 29 1 1 286 1 287 0.0 501
MS.gene67584.t1 MTR_5g023550 88.652 282 32 0 3 284 2 283 5.14e-176 487
MS.gene67584.t1 MTR_5g023320 87.719 285 31 2 1 284 1 282 1.67e-174 483
MS.gene67584.t1 MTR_5g023520 86.219 283 38 1 3 284 2 284 4.52e-173 479
MS.gene67584.t1 MTR_5g023580 82.867 286 45 2 1 282 1 286 1.24e-161 454
MS.gene67584.t1 MTR_8g074450 69.474 285 81 2 6 284 5 289 4.92e-137 389
MS.gene67584.t1 MTR_8g074510 66.316 285 88 3 6 284 5 287 6.43e-132 375
MS.gene67584.t1 MTR_8g074420 67.014 288 89 2 3 284 2 289 2.68e-129 369
MS.gene67584.t1 MTR_5g023460 86.486 185 21 1 100 284 11 191 7.63e-112 321
MS.gene67584.t1 MTR_8g074530 43.448 290 154 5 3 284 17 304 1.29e-65 207
MS.gene67584.t1 MTR_5g023290 36.667 300 172 4 3 284 9 308 1.39e-55 182
MS.gene67584.t1 MTR_5g023410 87.879 99 12 0 3 101 2 100 1.22e-50 166
MS.gene67584.t1 MTR_5g023650 35.811 296 174 4 4 284 10 304 1.20e-46 159
MS.gene67584.t1 MTR_8g074380 36.271 295 160 7 1 285 7 283 1.62e-46 159
MS.gene67584.t1 MTR_4g070530 34.386 285 176 4 7 283 13 294 1.86e-44 152
MS.gene67584.t1 MTR_3g116950 36.301 292 171 9 2 283 8 294 6.41e-43 149
MS.gene67584.t1 MTR_4g070570 31.757 296 183 9 2 283 11 301 1.06e-40 143
MS.gene67584.t1 MTR_4g070540 33.564 289 179 5 4 283 10 294 7.59e-40 140
MS.gene67584.t1 MTR_4g070590 35.374 294 173 8 3 283 10 299 1.91e-39 140
MS.gene67584.t1 MTR_3g116920 33.447 293 180 8 2 283 8 296 9.74e-37 132
MS.gene67584.t1 MTR_1g037820 30.769 234 144 5 52 284 9 225 1.92e-25 101
MS.gene67584.t1 MTR_3g115150 27.961 304 185 9 5 286 26 317 2.79e-24 100
MS.gene67584.t1 MTR_5g023540 52.174 69 30 1 163 231 1 66 8.87e-16 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67584.t1 AT4G35690 37.500 288 157 7 7 284 9 283 5.62e-54 177
MS.gene67584.t1 AT2G17080 36.170 282 157 6 3 283 2 261 2.71e-45 154
MS.gene67584.t1 AT2G17680 37.329 292 160 9 7 284 9 291 1.05e-44 153
MS.gene67584.t1 AT4G35710 36.770 291 154 9 7 284 9 282 2.27e-42 147
MS.gene67584.t1 AT2G17070 35.000 280 159 6 3 281 2 259 4.25e-42 145
MS.gene67584.t1 AT4G35200 33.452 281 157 7 3 282 2 253 1.21e-40 142
MS.gene67584.t1 AT4G35210 33.096 281 155 8 3 282 2 250 3.79e-38 135
MS.gene67584.t1 AT4G35680 30.820 305 180 10 3 284 13 309 1.29e-24 100
MS.gene67584.t1 AT4G35660 29.605 304 171 10 1 285 9 288 1.38e-23 97.8
MS.gene67584.t1 AT4G35720 29.712 313 184 6 6 284 14 324 3.70e-23 97.4
MS.gene67584.t1 AT1G76240 27.397 292 188 7 5 284 28 307 2.16e-22 94.7
MS.gene67584.t1 AT3G51400 31.081 296 169 10 3 285 2 275 2.31e-22 94.4
MS.gene67584.t1 AT1G76210 29.130 230 120 6 68 284 26 225 5.36e-18 81.3
MS.gene67584.t1 AT1G20520 29.224 219 121 7 75 284 32 225 4.22e-15 73.2
MS.gene67584.t1 AT3G51410 30.357 280 153 9 8 284 39 279 1.88e-13 69.3
MS.gene67584.t1 AT1G76220 28.620 297 154 11 2 285 5 256 1.38e-12 66.6

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATATGGAAAGTAAATT+TGG 0.150071 5.2:+14223159 MS.gene67584:CDS
GAACATCATGACTCTATCTT+AGG 0.153901 5.2:+14223548 MS.gene67584:CDS
AATCTCCCTTTCTCCTTCTA+AGG 0.157858 5.2:-14223053 None:intergenic
CATTGGAACCTTCACTTATA+AGG 0.178313 5.2:-14223422 None:intergenic
GCAATTGAAAGTATAGAAAA+TGG 0.264764 5.2:+14223477 MS.gene67584:CDS
CCACAAATTTCTCACCTTGA+TGG 0.264918 5.2:-14222930 None:intergenic
CACTTCACTCTTCCCTTAGA+AGG 0.345532 5.2:+14223040 MS.gene67584:CDS
TCCAACCAGGAAAGACAAAA+TGG 0.349375 5.2:-14223274 None:intergenic
AGAGAAAATATTTAGGCGTT+TGG 0.352912 5.2:+14223503 MS.gene67584:CDS
GTTTGAGCGAACATGGTAAT+TGG 0.359457 5.2:-14222719 None:intergenic
GAAATTTGTGGAAGAGTTGT+TGG 0.365610 5.2:+14222942 MS.gene67584:CDS
GATCACATCTTTAAAACATA+TGG 0.374642 5.2:+14223146 MS.gene67584:CDS
TTGTTCTATTCTTGTGGAGT+TGG 0.376848 5.2:-14222764 None:intergenic
TGTTCTATTCTTGTGGAGTT+GGG 0.378838 5.2:-14222763 None:intergenic
CAAAGTCAAAGGCAACAAAA+TGG 0.408302 5.2:+14223304 MS.gene67584:CDS
ACATGAAAGATTTGAGGCTT+TGG 0.411831 5.2:+14223449 MS.gene67584:CDS
GGATGGTTCAGTGAAAGTTT+TGG 0.423908 5.2:+14222963 MS.gene67584:CDS
TAGCTCTTGTTCTATTCTTG+TGG 0.426456 5.2:-14222770 None:intergenic
ATGGTTTAGAGAAAATATTT+AGG 0.435641 5.2:+14223496 MS.gene67584:CDS
CTCTTCCCTTAGAAGGAGAA+AGG 0.440449 5.2:+14223047 MS.gene67584:CDS
AAATCTTTCATGTGCAACAT+TGG 0.457668 5.2:-14223439 None:intergenic
CCATCAAGGTGAGAAATTTG+TGG 0.460090 5.2:+14222930 MS.gene67584:CDS
TGTTGCACATGAAAGATTTG+AGG 0.472738 5.2:+14223443 MS.gene67584:CDS
AACATCATGACTCTATCTTA+GGG 0.475458 5.2:+14223549 MS.gene67584:CDS
TGACAAAGTTGATGCAAAAG+AGG 0.510512 5.2:+14223334 MS.gene67584:CDS
ACAAATCTTCAAGCAAGTAA+AGG 0.525919 5.2:-14222852 None:intergenic
TTAAGAAGCCTTATAAGTGA+AGG 0.527521 5.2:+14223414 MS.gene67584:CDS
ATCTCCCTTTCTCCTTCTAA+GGG 0.536266 5.2:-14223052 None:intergenic
TGGATATCTGTGGCATCACA+AGG 0.539961 5.2:+14222983 MS.gene67584:CDS
TTCAAGCAAGTAAAGGCCAT+TGG 0.545983 5.2:-14222845 None:intergenic
GAAAACTGTTTGAGCGAACA+TGG 0.549885 5.2:-14222726 None:intergenic
AAGCTTGTTTCTCACCATCA+AGG 0.549955 5.2:+14222916 MS.gene67584:CDS
AGCTATGCAAAATGAAGACA+TGG 0.554695 5.2:+14222788 MS.gene67584:CDS
TCTTCCCTTAGAAGGAGAAA+GGG 0.559612 5.2:+14223048 MS.gene67584:CDS
GGTAACATGTGAAGAGAACA+TGG 0.580064 5.2:+14223356 MS.gene67584:CDS
TGGATCTTCATCAAAGTCAA+AGG 0.588491 5.2:+14223293 MS.gene67584:CDS
GACAAAGTTGATGCAAAAGA+GGG 0.591207 5.2:+14223335 MS.gene67584:CDS
AGTTATTACAGTTCTTAGAG+AGG 0.596107 5.2:+14223221 MS.gene67584:CDS
ATCAGATGTGGATGTTGATG+TGG 0.611639 5.2:-14222815 None:intergenic
TTTGTGGAAGAGTTGTTGGA+TGG 0.615570 5.2:+14222946 MS.gene67584:CDS
ACATCTGATTCAATTACCAA+TGG 0.619749 5.2:+14222829 MS.gene67584:CDS
ATTGGTAATTGAATCAGATG+TGG 0.626315 5.2:-14222827 None:intergenic
GGATATCTGTGGCATCACAA+GGG 0.651501 5.2:+14222984 MS.gene67584:CDS
ACTTTGATGAAGATCCAACC+AGG 0.658167 5.2:-14223287 None:intergenic
GAACTGTAATAACTGCGACA+AGG 0.693385 5.2:-14223212 None:intergenic
GCTATGCAAAATGAAGACAT+GGG 0.695872 5.2:+14222789 MS.gene67584:CDS
GAGTTGGGATGAGATTGAGA+AGG 0.749032 5.2:-14222748 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTTAGTGAAAAATTTATATT+CGG - chr5.2:14223100-14223119 None:intergenic 15.0%
!! AAACATATGGAAAGTAAATT+TGG + chr5.2:14223159-14223178 MS.gene67584:CDS 20.0%
!! ATGGTTTAGAGAAAATATTT+AGG + chr5.2:14223496-14223515 MS.gene67584:CDS 20.0%
!! GCAATTGAAAGTATAGAAAA+TGG + chr5.2:14223477-14223496 MS.gene67584:CDS 25.0%
!!! GATCACATCTTTAAAACATA+TGG + chr5.2:14223146-14223165 MS.gene67584:CDS 25.0%
AAATCTTTCATGTGCAACAT+TGG - chr5.2:14223442-14223461 None:intergenic 30.0%
ACAAATCTTCAAGCAAGTAA+AGG - chr5.2:14222855-14222874 None:intergenic 30.0%
ACATCTGATTCAATTACCAA+TGG + chr5.2:14222829-14222848 MS.gene67584:CDS 30.0%
AGAGAAAATATTTAGGCGTT+TGG + chr5.2:14223503-14223522 MS.gene67584:CDS 30.0%
AGTTATTACAGTTCTTAGAG+AGG + chr5.2:14223221-14223240 MS.gene67584:CDS 30.0%
! ATTGGTAATTGAATCAGATG+TGG - chr5.2:14222830-14222849 None:intergenic 30.0%
! TTAAGAAGCCTTATAAGTGA+AGG + chr5.2:14223414-14223433 MS.gene67584:CDS 30.0%
AGCTATGCAAAATGAAGACA+TGG + chr5.2:14222788-14222807 MS.gene67584:CDS 35.0%
CAAAGTCAAAGGCAACAAAA+TGG + chr5.2:14223304-14223323 MS.gene67584:CDS 35.0%
CATTGGAACCTTCACTTATA+AGG - chr5.2:14223425-14223444 None:intergenic 35.0%
GAAATTTGTGGAAGAGTTGT+TGG + chr5.2:14222942-14222961 MS.gene67584:CDS 35.0%
GCTATGCAAAATGAAGACAT+GGG + chr5.2:14222789-14222808 MS.gene67584:CDS 35.0%
TAGCTCTTGTTCTATTCTTG+TGG - chr5.2:14222773-14222792 None:intergenic 35.0%
TGGATCTTCATCAAAGTCAA+AGG + chr5.2:14223293-14223312 MS.gene67584:CDS 35.0%
TGTTCTATTCTTGTGGAGTT+GGG - chr5.2:14222766-14222785 None:intergenic 35.0%
TGTTGCACATGAAAGATTTG+AGG + chr5.2:14223443-14223462 MS.gene67584:CDS 35.0%
TTGTTCTATTCTTGTGGAGT+TGG - chr5.2:14222767-14222786 None:intergenic 35.0%
! ACATGAAAGATTTGAGGCTT+TGG + chr5.2:14223449-14223468 MS.gene67584:CDS 35.0%
! TCAATCCATTTTGTCTTTCC+TGG + chr5.2:14223269-14223288 MS.gene67584:CDS 35.0%
!! GACAAAGTTGATGCAAAAGA+GGG + chr5.2:14223335-14223354 MS.gene67584:CDS 35.0%
!! TGACAAAGTTGATGCAAAAG+AGG + chr5.2:14223334-14223353 MS.gene67584:CDS 35.0%
!!! GTGAAAGTTTTGGATATCTG+TGG + chr5.2:14222973-14222992 MS.gene67584:CDS 35.0%
AATCTCCCTTTCTCCTTCTA+AGG - chr5.2:14223056-14223075 None:intergenic 40.0%
ACTTTGATGAAGATCCAACC+AGG - chr5.2:14223290-14223309 None:intergenic 40.0%
ATCAGATGTGGATGTTGATG+TGG - chr5.2:14222818-14222837 None:intergenic 40.0%
ATCTCCCTTTCTCCTTCTAA+GGG - chr5.2:14223055-14223074 None:intergenic 40.0%
CCACAAATTTCTCACCTTGA+TGG - chr5.2:14222933-14222952 None:intergenic 40.0%
CCATCAAGGTGAGAAATTTG+TGG + chr5.2:14222930-14222949 MS.gene67584:CDS 40.0%
GAAAACTGTTTGAGCGAACA+TGG - chr5.2:14222729-14222748 None:intergenic 40.0%
GAACTGTAATAACTGCGACA+AGG - chr5.2:14223215-14223234 None:intergenic 40.0%
GGTAACATGTGAAGAGAACA+TGG + chr5.2:14223356-14223375 MS.gene67584:CDS 40.0%
GTTTGAGCGAACATGGTAAT+TGG - chr5.2:14222722-14222741 None:intergenic 40.0%
TCCAACCAGGAAAGACAAAA+TGG - chr5.2:14223277-14223296 None:intergenic 40.0%
TCTTCCCTTAGAAGGAGAAA+GGG + chr5.2:14223048-14223067 MS.gene67584:CDS 40.0%
TTCAAGCAAGTAAAGGCCAT+TGG - chr5.2:14222848-14222867 None:intergenic 40.0%
TTTGTGGAAGAGTTGTTGGA+TGG + chr5.2:14222946-14222965 MS.gene67584:CDS 40.0%
! AAGCTTGTTTCTCACCATCA+AGG + chr5.2:14222916-14222935 MS.gene67584:CDS 40.0%
! GGATGGTTCAGTGAAAGTTT+TGG + chr5.2:14222963-14222982 MS.gene67584:CDS 40.0%
! TCCATTTTGTCTTTCCTGGT+TGG + chr5.2:14223273-14223292 MS.gene67584:CDS 40.0%
CACTTCACTCTTCCCTTAGA+AGG + chr5.2:14223040-14223059 MS.gene67584:CDS 45.0%
CTCTTCCCTTAGAAGGAGAA+AGG + chr5.2:14223047-14223066 MS.gene67584:CDS 45.0%
GAGTTGGGATGAGATTGAGA+AGG - chr5.2:14222751-14222770 None:intergenic 45.0%
GGATATCTGTGGCATCACAA+GGG + chr5.2:14222984-14223003 MS.gene67584:CDS 45.0%
TGGATATCTGTGGCATCACA+AGG + chr5.2:14222983-14223002 MS.gene67584:CDS 45.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14222709 14223569 14222709 ID=MS.gene67584
chr5.2 mRNA 14222709 14223569 14222709 ID=MS.gene67584.t1;Parent=MS.gene67584
chr5.2 exon 14222709 14223569 14222709 ID=MS.gene67584.t1.exon1;Parent=MS.gene67584.t1
chr5.2 CDS 14222709 14223569 14222709 ID=cds.MS.gene67584.t1;Parent=MS.gene67584.t1
Gene Sequence

>MS.gene67584

ATGGCAAGCACCAATTACCATGTTCGCTCAAACAGTTTTCCTTCTCAATCTCATCCCAACTCCACAAGAATAGAACAAGAGCTATGCAAAATGAAGACATGGGAAGCCACATCAACATCCACATCTGATTCAATTACCAATGGCCTTTACTTGCTTGAAGATTTGTATATATCTTTAGAAAATCTTCTAAATATGACATCAACACAGAAGCTTGTTTCTCACCATCAAGGTGAGAAATTTGTGGAAGAGTTGTTGGATGGTTCAGTGAAAGTTTTGGATATCTGTGGCATCACAAGGGACACTATGTTACAAATTAAAGAAAATGTTCAGTCACTTCACTCTTCCCTTAGAAGGAGAAAGGGAGATTCAAGCATTGAAACAAGTGTAGCCGAATATAAATTTTTCACTAAGAAGATGAAGAAGAATGTCACAAAGTTGATCACATCTTTAAAACATATGGAAAGTAAATTTGGAGTTTCTTCACTTTTGAATCAAGATCAAGACCTTGTCGCAGTTATTACAGTTCTTAGAGAGGTCATTAAAATGAACTTGTCTATCTTTCAATCCATTTTGTCTTTCCTGGTTGGATCTTCATCAAAGTCAAAGGCAACAAAATGGTTGAAAGTGACAAAGTTGATGCAAAAGAGGGTAACATGTGAAGAGAACATGGAGAATTTCAATGAAGTGCAGTGTGTTGAAGCATCTTTAAGAAGCCTTATAAGTGAAGGTTCCAATGTTGCACATGAAAGATTTGAGGCTTTGGAGAATGCAATTGAAAGTATAGAAAATGGTTTAGAGAAAATATTTAGGCGTTTGGTTAAAACTAGAGTCTGTCTTTTGAACATCATGACTCTATCTTAG
Protein sequence

>MS.gene67584.t1

MASTNYHVRSNSFPSQSHPNSTRIEQELCKMKTWEATSTSTSDSITNGLYLLEDLYISLENLLNMTSTQKLVSHHQGEKFVEELLDGSVKVLDICGITRDTMLQIKENVQSLHSSLRRRKGDSSIETSVAEYKFFTKKMKKNVTKLITSLKHMESKFGVSSLLNQDQDLVAVITVLREVIKMNLSIFQSILSFLVGSSSKSKATKWLKVTKLMQKRVTCEENMENFNEVQCVEASLRSLISEGSNVAHERFEALENAIESIENGLEKIFRRLVKTRVCLLNIMTLS