AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene28914

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28914.t1 MTR_8g074510 95.139 288 14 0 1 288 1 288 0.0 556
MS.gene28914.t1 MTR_8g074450 86.897 290 36 1 1 288 1 290 0.0 513
MS.gene28914.t1 MTR_8g074420 82.759 290 48 1 1 288 1 290 1.34e-166 463
MS.gene28914.t1 MTR_5g023320 67.596 287 81 5 5 288 6 283 1.34e-127 364
MS.gene28914.t1 MTR_5g023550 65.398 289 92 3 1 287 1 283 1.47e-127 364
MS.gene28914.t1 MTR_5g023600 66.434 286 88 3 4 287 5 284 3.65e-127 363
MS.gene28914.t1 MTR_5g023520 64.138 290 97 2 1 288 1 285 5.90e-127 363
MS.gene28914.t1 MTR_5g023370 65.965 285 89 3 5 287 6 284 1.86e-126 362
MS.gene28914.t1 MTR_5g023390 65.614 285 90 3 5 287 6 284 5.04e-126 360
MS.gene28914.t1 MTR_5g023340 65.263 285 91 3 5 287 6 284 4.96e-125 358
MS.gene28914.t1 MTR_5g023350 65.035 286 92 3 4 287 5 284 1.58e-124 357
MS.gene28914.t1 MTR_5g023530 63.763 287 95 4 4 287 5 285 7.88e-122 350
MS.gene28914.t1 MTR_5g023470 62.718 287 98 4 4 287 5 285 1.33e-119 344
MS.gene28914.t1 MTR_5g023580 63.958 283 98 2 5 285 6 286 4.18e-117 341
MS.gene28914.t1 MTR_5g023460 63.021 192 61 3 97 288 11 192 5.31e-77 233
MS.gene28914.t1 MTR_8g074530 45.833 288 150 4 4 287 19 304 3.55e-75 232
MS.gene28914.t1 MTR_5g023290 40.333 300 165 4 2 287 9 308 2.77e-65 207
MS.gene28914.t1 MTR_4g070530 33.910 289 178 5 6 287 13 295 8.07e-44 151
MS.gene28914.t1 MTR_5g023650 32.534 292 183 4 8 287 15 304 1.92e-41 145
MS.gene28914.t1 MTR_8g074380 31.488 289 166 5 8 286 15 281 5.11e-39 139
MS.gene28914.t1 MTR_4g070570 29.412 289 197 3 5 287 15 302 1.09e-38 138
MS.gene28914.t1 MTR_5g023410 66.000 100 32 1 1 98 1 100 1.51e-37 132
MS.gene28914.t1 MTR_3g116950 34.694 294 171 10 5 287 12 295 1.56e-37 135
MS.gene28914.t1 MTR_3g116920 32.990 291 182 8 5 287 12 297 1.78e-37 134
MS.gene28914.t1 MTR_4g070540 31.944 288 187 5 6 288 13 296 2.68e-36 131
MS.gene28914.t1 MTR_4g070590 33.106 293 179 7 6 287 14 300 8.41e-36 130
MS.gene28914.t1 MTR_3g115150 27.597 308 183 10 3 287 25 315 2.91e-24 100
MS.gene28914.t1 MTR_1g037820 29.461 241 144 5 49 287 9 225 5.16e-24 97.4
MS.gene28914.t1 MTR_5g023540 48.438 64 29 1 163 226 4 63 6.28e-12 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene28914.t1 AT4G35690 35.570 298 163 6 1 287 4 283 5.92e-53 174
MS.gene28914.t1 AT2G17680 37.785 307 154 8 1 288 4 292 1.39e-46 158
MS.gene28914.t1 AT4G35710 37.838 296 158 9 1 287 4 282 1.20e-44 153
MS.gene28914.t1 AT2G17080 36.806 288 153 8 1 286 1 261 5.43e-43 148
MS.gene28914.t1 AT4G35200 37.193 285 147 10 1 285 1 253 2.78e-39 138
MS.gene28914.t1 AT2G17070 37.063 286 151 9 1 284 1 259 8.05e-39 137
MS.gene28914.t1 AT4G35210 34.386 285 152 9 1 285 1 250 3.81e-35 127
MS.gene28914.t1 AT4G35660 32.180 289 171 8 7 287 16 287 4.95e-29 112
MS.gene28914.t1 AT4G35720 31.746 315 179 10 5 287 14 324 2.30e-26 105
MS.gene28914.t1 AT3G51400 31.399 293 176 10 1 287 1 274 2.76e-21 91.3
MS.gene28914.t1 AT1G76240 27.211 294 194 6 3 288 27 308 3.29e-21 91.7
MS.gene28914.t1 AT4G35680 28.000 300 187 8 8 287 19 309 6.39e-19 85.5
MS.gene28914.t1 AT1G76210 26.222 225 143 3 63 287 24 225 9.05e-17 77.8
MS.gene28914.t1 AT3G51410 30.249 281 154 6 8 287 40 279 4.54e-16 77.0
MS.gene28914.t1 AT1G20520 25.893 224 141 4 65 287 26 225 9.83e-12 63.9

Find 44 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGGCATGTCTTCAATTT+TGG 0.093147 8.2:-30352976 MS.gene28914:CDS
TTCACTTAAAAGAGTGTTTA+AGG 0.140662 8.2:+30352737 None:intergenic
AAGCAAATGGATAGTAAATT+TGG 0.146612 8.2:-30352993 MS.gene28914:CDS
AGATAGTTCAATGAGAATTT+TGG 0.199075 8.2:-30353189 MS.gene28914:CDS
ATGATTTGGAGAACTTATTT+AGG 0.260717 8.2:-30352638 MS.gene28914:CDS
TGAAACTATTGAGAATGATT+TGG 0.295462 8.2:-30352652 MS.gene28914:CDS
AATATATAAATCATCTATTA+AGG 0.302191 8.2:+30353295 None:intergenic
CAATGCATTTCTCACCTCTA+TGG 0.309012 8.2:+30353222 None:intergenic
AAGCCCTTCACTCTTGTCTA+AGG 0.365459 8.2:-30353115 MS.gene28914:CDS
AGATGATTTATATATTTCAT+TGG 0.368321 8.2:-30353288 MS.gene28914:CDS
ACACTCTAATCAAAGAATAA+AGG 0.396201 8.2:+30352940 None:intergenic
ACATGAAAGATTAGAGGCTT+TGG 0.438324 8.2:-30352685 MS.gene28914:CDS
ACATCATGCTCCATTCATAT+TGG 0.455596 8.2:-30353323 MS.gene28914:CDS
TGGATATTTGTGGCATCACT+AGG 0.456088 8.2:-30353169 MS.gene28914:CDS
AGATCTAGAAGGCAAACTAA+TGG 0.456112 8.2:+30353409 None:intergenic
GGCTGCACATGAAAGATTAG+AGG 0.456678 8.2:-30352691 MS.gene28914:CDS
GATCGCATCTTTGAAGCAAA+TGG 0.471046 8.2:-30353006 MS.gene28914:CDS
ATGGTGAGAAATCACTTGTT+GGG 0.479630 8.2:+30353241 None:intergenic
TATGGTGAGAAATCACTTGT+TGG 0.484011 8.2:+30353240 None:intergenic
TAAGGAAAGACCAATATGAA+TGG 0.496985 8.2:+30353313 None:intergenic
GGATACAATTCTTCAAATCA+AGG 0.500801 8.2:-30353147 MS.gene28914:CDS
TCTTGTCTAAGGAGAAGAAA+AGG 0.505857 8.2:-30353104 MS.gene28914:CDS
GTGCTAGGATGAGATCTAGA+AGG 0.513144 8.2:+30353398 None:intergenic
TTCTCCTTAGACAAGAGTGA+AGG 0.531189 8.2:+30353111 None:intergenic
TCATCTACTCTAATTGTGCT+AGG 0.541777 8.2:+30353383 None:intergenic
ACCATGGCAATCAAGTATCA+TGG 0.555053 8.2:-30353437 MS.gene28914:CDS
TCTCCTTAGACAAGAGTGAA+GGG 0.564139 8.2:+30353112 None:intergenic
ATTGAGAAATTATATTCAAG+AGG 0.564623 8.2:+30352592 None:intergenic
GGATATTTGTGGCATCACTA+GGG 0.577026 8.2:-30353168 MS.gene28914:CDS
AACAAGCTCAAGACATGGGA+AGG 0.580057 8.2:-30353353 MS.gene28914:CDS
AGGTGAGAAATGCATTGAAG+AGG 0.582191 8.2:-30353216 MS.gene28914:CDS
TTGAGAAATTATATTCAAGA+GGG 0.584586 8.2:+30352593 None:intergenic
TAATCAAAGAATAAAGGTGA+TGG 0.595163 8.2:+30352946 None:intergenic
TAGCACAATTAGAGTAGATG+AGG 0.604276 8.2:-30353381 MS.gene28914:CDS
TTTAGCAACAAATTTCCACT+TGG 0.605269 8.2:+30352833 None:intergenic
TACTAATGGAGAAAACATGC+AGG 0.606065 8.2:-30352712 MS.gene28914:CDS
ATTGGATGATCTTCTCAACA+TGG 0.611714 8.2:-30353270 MS.gene28914:CDS
AATTGAACAAGCTCAAGACA+TGG 0.618716 8.2:-30353358 MS.gene28914:CDS
CAAGTGATTTCTCACCATAG+AGG 0.629127 8.2:-30353236 MS.gene28914:CDS
ATTGAACAAGCTCAAGACAT+GGG 0.630590 8.2:-30353357 MS.gene28914:CDS
GTTGCTAAAATGATGCACAA+AGG 0.639952 8.2:-30352819 MS.gene28914:CDS
TTCAAGTGTTGAAAGAAGTG+TGG 0.668403 8.2:-30353078 MS.gene28914:CDS
CAAAGTCAAAGACAACCAAG+TGG 0.668953 8.2:-30352848 MS.gene28914:CDS
ACCATGATACTTGATTGCCA+TGG 0.702181 8.2:+30353436 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAAATCATCTATTA+AGG + chr8.2:30352732-30352751 None:intergenic 10.0%
!! AGATGATTTATATATTTCAT+TGG - chr8.2:30352736-30352755 MS.gene28914:CDS 15.0%
!! ATTGAGAAATTATATTCAAG+AGG + chr8.2:30353435-30353454 None:intergenic 20.0%
!! TTGAGAAATTATATTCAAGA+GGG + chr8.2:30353434-30353453 None:intergenic 20.0%
! AAGCAAATGGATAGTAAATT+TGG - chr8.2:30353031-30353050 MS.gene28914:CDS 25.0%
! ACACTCTAATCAAAGAATAA+AGG + chr8.2:30353087-30353106 None:intergenic 25.0%
! TAATCAAAGAATAAAGGTGA+TGG + chr8.2:30353081-30353100 None:intergenic 25.0%
! TGAAACTATTGAGAATGATT+TGG - chr8.2:30353372-30353391 MS.gene28914:CDS 25.0%
!! AGATAGTTCAATGAGAATTT+TGG - chr8.2:30352835-30352854 MS.gene28914:CDS 25.0%
!! ATGAGAATTTTGGATATTTG+TGG - chr8.2:30352845-30352864 MS.gene28914:CDS 25.0%
!! ATGATTTGGAGAACTTATTT+AGG - chr8.2:30353386-30353405 MS.gene28914:CDS 25.0%
!!! TTAAACACTCTTTTAAGTGA+AGG - chr8.2:30353289-30353308 MS.gene28914:CDS 25.0%
!!! TTCACTTAAAAGAGTGTTTA+AGG + chr8.2:30353290-30353309 None:intergenic 25.0%
GGATACAATTCTTCAAATCA+AGG - chr8.2:30352877-30352896 MS.gene28914:CDS 30.0%
TAAGGAAAGACCAATATGAA+TGG + chr8.2:30352714-30352733 None:intergenic 30.0%
TTTAGCAACAAATTTCCACT+TGG + chr8.2:30353194-30353213 None:intergenic 30.0%
! ATTTGGCATGTCTTCAATTT+TGG - chr8.2:30353048-30353067 MS.gene28914:CDS 30.0%
!!! CTTTTAAGTGAAGGTACTAA+TGG - chr8.2:30353298-30353317 MS.gene28914:CDS 30.0%
AATTGAACAAGCTCAAGACA+TGG - chr8.2:30352666-30352685 MS.gene28914:CDS 35.0%
ACATCATGCTCCATTCATAT+TGG - chr8.2:30352701-30352720 MS.gene28914:CDS 35.0%
ACATGAAAGATTAGAGGCTT+TGG - chr8.2:30353339-30353358 MS.gene28914:CDS 35.0%
AGATCTAGAAGGCAAACTAA+TGG + chr8.2:30352618-30352637 None:intergenic 35.0%
ATGGTGAGAAATCACTTGTT+GGG + chr8.2:30352786-30352805 None:intergenic 35.0%
ATTGAACAAGCTCAAGACAT+GGG - chr8.2:30352667-30352686 MS.gene28914:CDS 35.0%
ATTGGATGATCTTCTCAACA+TGG - chr8.2:30352754-30352773 MS.gene28914:CDS 35.0%
GTTGCTAAAATGATGCACAA+AGG - chr8.2:30353205-30353224 MS.gene28914:CDS 35.0%
TACTAATGGAGAAAACATGC+AGG - chr8.2:30353312-30353331 MS.gene28914:CDS 35.0%
TAGCACAATTAGAGTAGATG+AGG - chr8.2:30352643-30352662 MS.gene28914:CDS 35.0%
TATGGTGAGAAATCACTTGT+TGG + chr8.2:30352787-30352806 None:intergenic 35.0%
TCATCTACTCTAATTGTGCT+AGG + chr8.2:30352644-30352663 None:intergenic 35.0%
TCTTGTCTAAGGAGAAGAAA+AGG - chr8.2:30352920-30352939 MS.gene28914:CDS 35.0%
!! TTCAAGTGTTGAAAGAAGTG+TGG - chr8.2:30352946-30352965 MS.gene28914:CDS 35.0%
AGGTGAGAAATGCATTGAAG+AGG - chr8.2:30352808-30352827 MS.gene28914:CDS 40.0%
CAAAGTCAAAGACAACCAAG+TGG - chr8.2:30353176-30353195 MS.gene28914:CDS 40.0%
CAAGTGATTTCTCACCATAG+AGG - chr8.2:30352788-30352807 MS.gene28914:CDS 40.0%
CAATGCATTTCTCACCTCTA+TGG + chr8.2:30352805-30352824 None:intergenic 40.0%
GGATATTTGTGGCATCACTA+GGG - chr8.2:30352856-30352875 MS.gene28914:CDS 40.0%
TCTCCTTAGACAAGAGTGAA+GGG + chr8.2:30352915-30352934 None:intergenic 40.0%
TGGATATTTGTGGCATCACT+AGG - chr8.2:30352855-30352874 MS.gene28914:CDS 40.0%
TTCTCCTTAGACAAGAGTGA+AGG + chr8.2:30352916-30352935 None:intergenic 40.0%
!! GATCGCATCTTTGAAGCAAA+TGG - chr8.2:30353018-30353037 MS.gene28914:CDS 40.0%
AACAAGCTCAAGACATGGGA+AGG - chr8.2:30352671-30352690 MS.gene28914:CDS 45.0%
AAGCCCTTCACTCTTGTCTA+AGG - chr8.2:30352909-30352928 MS.gene28914:CDS 45.0%
GGCTGCACATGAAAGATTAG+AGG - chr8.2:30353333-30353352 MS.gene28914:CDS 45.0%
GTGCTAGGATGAGATCTAGA+AGG + chr8.2:30352629-30352648 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 30352590 30353456 30352590 ID=MS.gene28914
chr8.2 mRNA 30352590 30353456 30352590 ID=MS.gene28914.t1;Parent=MS.gene28914
chr8.2 exon 30352590 30353456 30352590 ID=MS.gene28914.t1.exon1;Parent=MS.gene28914.t1
chr8.2 CDS 30352590 30353456 30352590 ID=cds.MS.gene28914.t1;Parent=MS.gene28914.t1
Gene Sequence

>MS.gene28914

ATGGCAATCAAGTATCATGGTCGTTCCATTAGTTTGCCTTCTAGATCTCATCCTAGCACAATTAGAGTAGATGAGGAATTGAACAAGCTCAAGACATGGGAAGGAACATCTACATCATGCTCCATTCATATTGGTCTTTCCTTAATAGATGATTTATATATTTCATTGGATGATCTTCTCAACATGGCATCAACCCAACAAGTGATTTCTCACCATAGAGGTGAGAAATGCATTGAAGAGGTGTTAGATAGTTCAATGAGAATTTTGGATATTTGTGGCATCACTAGGGATACAATTCTTCAAATCAAGGAAAATGTTCAAGCCCTTCACTCTTGTCTAAGGAGAAGAAAAGGTGATTCAAGTGTTGAAAGAAGTGTGGTAGAATACAAATTCTTCACAAAAAAGATGAAAAAAAATGTCAACAAGTTGATCGCATCTTTGAAGCAAATGGATAGTAAATTTGGCATGTCTTCAATTTTGGAACTTGACCATCACCTTTATTCTTTGATTAGAGTGTTTAAAGAAGTCATTGCAATGAATTTGTCTATTTTTCAAATCATTTTGTCATTCTTGACTATGTCTTCATCAAAGTCAAAGACAACCAAGTGGAAATTTGTTGCTAAAATGATGCACAAAGGAGTCATAGCATGTGAAGATAACTCAGATAATGTCAATGAGTTTTTGTGTGTTGAAGCTACCTTAAACACTCTTTTAAGTGAAGGTACTAATGGAGAAAACATGCAGGCTGCACATGAAAGATTAGAGGCTTTGGAGAATGCAATTGAAACTATTGAGAATGATTTGGAGAACTTATTTAGGCGCTTAATAAAATCTAGAGCTTCCCTCTTGAATATAATTTCTCAATAG
Protein sequence

>MS.gene28914.t1

MAIKYHGRSISLPSRSHPSTIRVDEELNKLKTWEGTSTSCSIHIGLSLIDDLYISLDDLLNMASTQQVISHHRGEKCIEEVLDSSMRILDICGITRDTILQIKENVQALHSCLRRRKGDSSVERSVVEYKFFTKKMKKNVNKLIASLKQMDSKFGMSSILELDHHLYSLIRVFKEVIAMNLSIFQIILSFLTMSSSKSKTTKWKFVAKMMHKGVIACEDNSDNVNEFLCVEATLNTLLSEGTNGENMQAAHERLEALENAIETIENDLENLFRRLIKSRASLLNIISQ