AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67582

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67582.t1 MTR_5g023390 90.175 285 26 2 1 284 1 284 2.50e-180 498
MS.gene67582.t1 MTR_5g023350 91.228 285 23 2 1 284 1 284 1.72e-179 496
MS.gene67582.t1 MTR_5g023550 90.175 285 27 1 1 285 1 284 3.89e-179 495
MS.gene67582.t1 MTR_5g023520 88.462 286 31 2 1 285 1 285 2.69e-178 493
MS.gene67582.t1 MTR_5g023600 90.526 285 25 2 1 284 1 284 5.21e-178 492
MS.gene67582.t1 MTR_5g023370 89.123 285 29 2 1 284 1 284 1.22e-177 491
MS.gene67582.t1 MTR_5g023340 88.772 285 30 2 1 284 1 284 5.80e-176 487
MS.gene67582.t1 MTR_5g023530 87.943 282 34 0 3 284 4 285 2.24e-175 485
MS.gene67582.t1 MTR_5g023470 86.879 282 37 0 3 284 4 285 4.93e-173 479
MS.gene67582.t1 MTR_5g023320 87.805 287 29 4 1 285 1 283 2.60e-171 475
MS.gene67582.t1 MTR_5g023580 83.275 287 42 4 1 282 1 286 1.49e-157 443
MS.gene67582.t1 MTR_8g074450 68.041 291 86 3 1 285 1 290 9.29e-136 385
MS.gene67582.t1 MTR_8g074510 66.323 291 89 4 1 285 1 288 4.46e-132 376
MS.gene67582.t1 MTR_8g074420 67.354 291 88 3 1 285 1 290 3.39e-129 369
MS.gene67582.t1 MTR_5g023460 87.166 187 19 2 99 285 11 192 9.99e-112 321
MS.gene67582.t1 MTR_8g074530 44.138 290 153 5 2 284 17 304 6.10e-67 211
MS.gene67582.t1 MTR_5g023290 37.209 301 170 5 2 284 9 308 3.39e-55 181
MS.gene67582.t1 MTR_5g023410 91.000 100 9 0 1 100 1 100 3.77e-53 172
MS.gene67582.t1 MTR_8g074380 36.713 286 154 7 7 283 14 281 2.98e-46 158
MS.gene67582.t1 MTR_4g070530 33.793 290 184 5 1 284 8 295 3.58e-44 152
MS.gene67582.t1 MTR_5g023650 35.495 293 172 6 7 284 14 304 1.79e-43 150
MS.gene67582.t1 MTR_3g116950 37.113 291 167 10 3 283 10 294 1.00e-41 145
MS.gene67582.t1 MTR_4g070590 36.552 290 168 8 6 283 14 299 1.71e-40 142
MS.gene67582.t1 MTR_4g070540 32.192 292 184 5 3 285 10 296 4.12e-40 141
MS.gene67582.t1 MTR_4g070570 31.293 294 184 9 3 283 13 301 1.55e-37 135
MS.gene67582.t1 MTR_3g116920 34.354 294 173 11 3 283 10 296 5.64e-36 130
MS.gene67582.t1 MTR_3g115150 27.815 302 183 9 5 284 27 315 7.45e-26 104
MS.gene67582.t1 MTR_1g037820 31.489 235 142 5 51 284 9 225 6.68e-25 99.8
MS.gene67582.t1 MTR_5g023540 58.621 58 21 1 173 230 11 65 2.35e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67582.t1 AT4G35690 37.543 293 161 7 1 284 4 283 3.08e-54 177
MS.gene67582.t1 AT2G17080 36.972 284 155 6 1 283 1 261 1.58e-48 162
MS.gene67582.t1 AT2G17070 36.525 282 155 6 1 281 1 259 9.51e-47 157
MS.gene67582.t1 AT2G17680 38.284 303 155 10 1 285 4 292 2.96e-46 157
MS.gene67582.t1 AT4G35200 35.336 283 152 8 1 282 1 253 1.69e-44 151
MS.gene67582.t1 AT4G35710 36.667 300 155 10 1 285 4 283 4.46e-43 149
MS.gene67582.t1 AT4G35210 34.276 283 152 8 1 282 1 250 4.64e-42 145
MS.gene67582.t1 AT1G76240 26.552 290 194 7 5 285 29 308 7.26e-25 101
MS.gene67582.t1 AT4G35720 31.329 316 176 9 5 284 14 324 1.63e-24 100
MS.gene67582.t1 AT4G35680 30.667 300 178 8 7 284 18 309 6.56e-24 99.0
MS.gene67582.t1 AT4G35660 31.419 296 159 11 8 284 17 287 2.66e-23 97.1
MS.gene67582.t1 AT3G51400 30.976 297 169 10 1 284 1 274 1.03e-19 87.0
MS.gene67582.t1 AT1G76210 28.509 228 137 7 61 284 20 225 1.93e-16 77.0
MS.gene67582.t1 AT3G51410 31.673 281 149 8 7 284 39 279 4.55e-16 77.0
MS.gene67582.t1 AT1G20520 26.531 245 146 6 47 284 8 225 1.14e-12 66.6

Find 44 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACATATGGAAACTAAATT+TGG 0.157953 5.2:-14214471 MS.gene67582:CDS
GCAATTGAAAGTATAGAAAA+TGG 0.264764 5.2:-14214150 MS.gene67582:CDS
GCCAACCAAGAAAGACAAAA+TGG 0.341438 5.2:+14214353 None:intergenic
TGAAGATTTGTATATTTCAT+TGG 0.354547 5.2:-14214766 MS.gene67582:CDS
AGAGAACATATTTAGGCGTT+TGG 0.368589 5.2:-14214124 MS.gene67582:CDS
GATCACATCTTTAAAACATA+TGG 0.374642 5.2:-14214484 MS.gene67582:CDS
CACTTCACTCCGCTCTTAGA+AGG 0.374965 5.2:-14214590 MS.gene67582:CDS
ATGGTTTAGAGAACATATTT+AGG 0.402533 5.2:-14214131 MS.gene67582:CDS
CAAAGTCAAAGGCAACAAAA+TGG 0.408302 5.2:-14214323 MS.gene67582:CDS
TGTTCAATTCTAGTGGTGCT+TGG 0.418571 5.2:+14214867 None:intergenic
GAAAATTGTGGAAGAGTTGT+TGG 0.420097 5.2:-14214688 MS.gene67582:CDS
ACATGAAAGATTTGTGGCTT+TGG 0.427553 5.2:-14214178 MS.gene67582:CDS
CATTAGAACCTTCACTTGAA+AGG 0.429263 5.2:+14214205 None:intergenic
GGAAGAGTTGTTGGATGGTT+CGG 0.441396 5.2:-14214679 MS.gene67582:CDS
ATTAGAACCTTCACTTGAAA+GGG 0.442145 5.2:+14214206 None:intergenic
GTTGCAGTGATTACAGTTCT+CGG 0.442595 5.2:-14214411 MS.gene67582:CDS
CTCACCTTGATGGTGAGAAA+CGG 0.477848 5.2:+14214710 None:intergenic
CTCCGCTCTTAGAAGGAGAA+AGG 0.483537 5.2:-14214583 MS.gene67582:CDS
TGTTGCACATGAAAGATTTG+TGG 0.489205 5.2:-14214184 MS.gene67582:CDS
ACATATGTCATCAACACAAA+AGG 0.492531 5.2:-14214733 MS.gene67582:CDS
TAACTCTTGTTCAATTCTAG+TGG 0.494436 5.2:+14214860 None:intergenic
ACAAATCTTCAAGAAAGAAA+AGG 0.508287 5.2:+14214778 None:intergenic
TGACAAAGTTGATGCAAAAG+AGG 0.510512 5.2:-14214293 MS.gene67582:CDS
TTAAGAACCCTTTCAAGTGA+AGG 0.514521 5.2:-14214213 MS.gene67582:CDS
AGATTTGTATATTTCATTGG+AGG 0.519503 5.2:-14214763 MS.gene67582:CDS
TCCGCTCTTAGAAGGAGAAA+GGG 0.536323 5.2:-14214582 MS.gene67582:CDS
TAGATATATGTGGCATCACA+AGG 0.540397 5.2:-14214647 MS.gene67582:CDS
CCATCAAGGTGAGAAAATTG+TGG 0.548327 5.2:-14214700 MS.gene67582:CDS
TTCAAGAAAGAAAAGGCCAT+TGG 0.559275 5.2:+14214785 None:intergenic
GAAAACTATTTGAGCGAACA+TGG 0.573814 5.2:+14214904 None:intergenic
GGTAACATGTGAAGAGAACA+TGG 0.580064 5.2:-14214271 MS.gene67582:CDS
TGGCTCTTCATCAAAGTCAA+AGG 0.589744 5.2:-14214334 MS.gene67582:CDS
GACAAAGTTGATGCAAAAGA+GGG 0.591207 5.2:-14214292 MS.gene67582:CDS
TCCCTTTCTCCTTCTAAGAG+CGG 0.591308 5.2:+14214581 None:intergenic
ATTGTGGAAGAGTTGTTGGA+TGG 0.606512 5.2:-14214684 MS.gene67582:CDS
ATCAGATGTGGATGTTGATG+TGG 0.611639 5.2:+14214815 None:intergenic
ACATCTGATTCAATTACCAA+TGG 0.619749 5.2:-14214801 MS.gene67582:CDS
ATTGGTAATTGAATCAGATG+TGG 0.626315 5.2:+14214803 None:intergenic
AAGGCCGTTTCTCACCATCA+AGG 0.642576 5.2:-14214714 MS.gene67582:CDS
ATGATCTTGATTCAAAAGTG+AGG 0.650994 5.2:+14214443 None:intergenic
GAACTGTAATCACTGCAACA+AGG 0.659306 5.2:+14214415 None:intergenic
AGATATATGTGGCATCACAA+GGG 0.670882 5.2:-14214646 MS.gene67582:CDS
GTGCTTGGATGAGATTGAGA+AGG 0.684385 5.2:+14214882 None:intergenic
AGTGATTACAGTTCTCGGAG+AGG 0.692962 5.2:-14214406 MS.gene67582:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACATATGGAAACTAAATT+TGG - chr5.2:14214530-14214549 MS.gene67582:CDS 20.0%
!! TGAAGATTTGTATATTTCAT+TGG - chr5.2:14214235-14214254 MS.gene67582:CDS 20.0%
!!! ATTTTCTTTAATCTGTAACA+TGG + chr5.2:14214384-14214403 None:intergenic 20.0%
! ACAAATCTTCAAGAAAGAAA+AGG + chr5.2:14214226-14214245 None:intergenic 25.0%
! AGATTTGTATATTTCATTGG+AGG - chr5.2:14214238-14214257 MS.gene67582:CDS 25.0%
! AGTTAACAAAAATGAAGACA+TGG - chr5.2:14214159-14214178 MS.gene67582:CDS 25.0%
! ATGGTTTAGAGAACATATTT+AGG - chr5.2:14214870-14214889 MS.gene67582:CDS 25.0%
! GTTAACAAAAATGAAGACAT+GGG - chr5.2:14214160-14214179 MS.gene67582:CDS 25.0%
!! GCAATTGAAAGTATAGAAAA+TGG - chr5.2:14214851-14214870 MS.gene67582:CDS 25.0%
!! GTGAAACTTTTAGATATATG+TGG - chr5.2:14214344-14214363 MS.gene67582:CDS 25.0%
!!! GATCACATCTTTAAAACATA+TGG - chr5.2:14214517-14214536 MS.gene67582:CDS 25.0%
ACATATGTCATCAACACAAA+AGG - chr5.2:14214268-14214287 MS.gene67582:CDS 30.0%
ACATCTGATTCAATTACCAA+TGG - chr5.2:14214200-14214219 MS.gene67582:CDS 30.0%
ATGATCTTGATTCAAAAGTG+AGG + chr5.2:14214561-14214580 None:intergenic 30.0%
ATTAGAACCTTCACTTGAAA+GGG + chr5.2:14214798-14214817 None:intergenic 30.0%
TAACTCTTGTTCAATTCTAG+TGG + chr5.2:14214144-14214163 None:intergenic 30.0%
! ATTGGTAATTGAATCAGATG+TGG + chr5.2:14214201-14214220 None:intergenic 30.0%
! TCAATCCATTTTGTCTTTCT+TGG - chr5.2:14214643-14214662 MS.gene67582:CDS 30.0%
ACATGAAAGATTTGTGGCTT+TGG - chr5.2:14214823-14214842 MS.gene67582:CDS 35.0%
AGAGAACATATTTAGGCGTT+TGG - chr5.2:14214877-14214896 MS.gene67582:CDS 35.0%
AGATATATGTGGCATCACAA+GGG - chr5.2:14214355-14214374 MS.gene67582:CDS 35.0%
CAAAGTCAAAGGCAACAAAA+TGG - chr5.2:14214678-14214697 MS.gene67582:CDS 35.0%
CATTAGAACCTTCACTTGAA+AGG + chr5.2:14214799-14214818 None:intergenic 35.0%
GAAAACTATTTGAGCGAACA+TGG + chr5.2:14214100-14214119 None:intergenic 35.0%
GAAAATTGTGGAAGAGTTGT+TGG - chr5.2:14214313-14214332 MS.gene67582:CDS 35.0%
TAGATATATGTGGCATCACA+AGG - chr5.2:14214354-14214373 MS.gene67582:CDS 35.0%
TGTTGCACATGAAAGATTTG+TGG - chr5.2:14214817-14214836 MS.gene67582:CDS 35.0%
TTCAAGAAAGAAAAGGCCAT+TGG + chr5.2:14214219-14214238 None:intergenic 35.0%
! TCCATTTTGTCTTTCTTGGT+TGG - chr5.2:14214647-14214666 MS.gene67582:CDS 35.0%
! TTAAGAACCCTTTCAAGTGA+AGG - chr5.2:14214788-14214807 MS.gene67582:CDS 35.0%
!! GACAAAGTTGATGCAAAAGA+GGG - chr5.2:14214709-14214728 MS.gene67582:CDS 35.0%
!! TGACAAAGTTGATGCAAAAG+AGG - chr5.2:14214708-14214727 MS.gene67582:CDS 35.0%
ATCAGATGTGGATGTTGATG+TGG + chr5.2:14214189-14214208 None:intergenic 40.0%
ATTGTGGAAGAGTTGTTGGA+TGG - chr5.2:14214317-14214336 MS.gene67582:CDS 40.0%
CCATCAAGGTGAGAAAATTG+TGG - chr5.2:14214301-14214320 MS.gene67582:CDS 40.0%
GAACTGTAATCACTGCAACA+AGG + chr5.2:14214589-14214608 None:intergenic 40.0%
GCCAACCAAGAAAGACAAAA+TGG + chr5.2:14214651-14214670 None:intergenic 40.0%
GGTAACATGTGAAGAGAACA+TGG - chr5.2:14214730-14214749 MS.gene67582:CDS 40.0%
GTTGCAGTGATTACAGTTCT+CGG - chr5.2:14214590-14214609 MS.gene67582:CDS 40.0%
TGGCTCTTCATCAAAGTCAA+AGG - chr5.2:14214667-14214686 MS.gene67582:CDS 40.0%
TGTTCAATTCTAGTGGTGCT+TGG + chr5.2:14214137-14214156 None:intergenic 40.0%
! CCACAATTTTCTCACCTTGA+TGG + chr5.2:14214304-14214323 None:intergenic 40.0%
AGTGATTACAGTTCTCGGAG+AGG - chr5.2:14214595-14214614 MS.gene67582:CDS 45.0%
GGAAGAGTTGTTGGATGGTT+CGG - chr5.2:14214322-14214341 MS.gene67582:CDS 45.0%
GTGCTTGGATGAGATTGAGA+AGG + chr5.2:14214122-14214141 None:intergenic 45.0%
TCCGCTCTTAGAAGGAGAAA+GGG - chr5.2:14214419-14214438 MS.gene67582:CDS 45.0%
! TCCCTTTCTCCTTCTAAGAG+CGG + chr5.2:14214423-14214442 None:intergenic 45.0%
!! CTCACCTTGATGGTGAGAAA+CGG + chr5.2:14214294-14214313 None:intergenic 45.0%
AAGGCCGTTTCTCACCATCA+AGG - chr5.2:14214287-14214306 MS.gene67582:CDS 50.0%
CACTTCACTCCGCTCTTAGA+AGG - chr5.2:14214411-14214430 MS.gene67582:CDS 50.0%
CTCCGCTCTTAGAAGGAGAA+AGG - chr5.2:14214418-14214437 MS.gene67582:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14214083 14214940 14214083 ID=MS.gene67582
chr5.2 mRNA 14214083 14214940 14214083 ID=MS.gene67582.t1;Parent=MS.gene67582
chr5.2 exon 14214083 14214940 14214083 ID=MS.gene67582.t1.exon1;Parent=MS.gene67582.t1
chr5.2 CDS 14214083 14214940 14214083 ID=cds.MS.gene67582.t1;Parent=MS.gene67582.t1
Gene Sequence

>MS.gene67582

ATGGCAAGCAAATACCATGTTCGCTCAAATAGTTTTCCTTCTCAATCTCATCCAAGCACCACTAGAATTGAACAAGAGTTAACAAAAATGAAGACATGGGAAGCCACATCAACATCCACATCTGATTCAATTACCAATGGCCTTTTCTTTCTTGAAGATTTGTATATTTCATTGGAGGATCTTCTACATATGTCATCAACACAAAAGGCCGTTTCTCACCATCAAGGTGAGAAAATTGTGGAAGAGTTGTTGGATGGTTCGGTGAAACTTTTAGATATATGTGGCATCACAAGGGACACCATGTTACAGATTAAAGAAAATGTTCAATCACTTCACTCCGCTCTTAGAAGGAGAAAGGGAGATTCAAGCATCGAAACAAGTGTAGTTGAATATAAATTATTCACAAAGAAGATGAAGAAGAATGTCACAAAGTTGATCACATCTTTAAAACATATGGAAACTAAATTTGGAGCATCCTCACTTTTGAATCAAGATCATCAAGACCTTGTTGCAGTGATTACAGTTCTCGGAGAGGTGATTGCAATGAACATCTCTATCTTTCAATCCATTTTGTCTTTCTTGGTTGGCTCTTCATCAAAGTCAAAGGCAACAAAATGGTTGAAAGTGACAAAGTTGATGCAAAAGAGGGTAACATGTGAAGAGAACATGGAGAATTTCAATGAATTGCAGTGTGTCGAAGCATCTTTAAGAACCCTTTCAAGTGAAGGTTCTAATGTTGCACATGAAAGATTTGTGGCTTTGGAGAATGCAATTGAAAGTATAGAAAATGGTTTAGAGAACATATTTAGGCGTTTGGTTAAAACTAGAGTCTGTCTTTTGAACATCATGACTCAATAG
Protein sequence

>MS.gene67582.t1

MASKYHVRSNSFPSQSHPSTTRIEQELTKMKTWEATSTSTSDSITNGLFFLEDLYISLEDLLHMSSTQKAVSHHQGEKIVEELLDGSVKLLDICGITRDTMLQIKENVQSLHSALRRRKGDSSIETSVVEYKLFTKKMKKNVTKLITSLKHMETKFGASSLLNQDHQDLVAVITVLGEVIAMNISIFQSILSFLVGSSSKSKATKWLKVTKLMQKRVTCEENMENFNELQCVEASLRTLSSEGSNVAHERFVALENAIESIENGLENIFRRLVKTRVCLLNIMTQ