AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene67583

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67583.t1 MTR_5g023370 93.357 286 19 0 1 286 1 286 0.0 520
MS.gene67583.t1 MTR_5g023390 91.958 286 23 0 1 286 1 286 0.0 513
MS.gene67583.t1 MTR_5g023350 92.308 286 22 0 1 286 1 286 0.0 511
MS.gene67583.t1 MTR_5g023340 91.958 286 23 0 1 286 1 286 0.0 509
MS.gene67583.t1 MTR_5g023600 91.608 286 24 0 1 286 1 286 0.0 509
MS.gene67583.t1 MTR_5g023550 89.085 284 30 1 1 284 1 283 1.61e-176 488
MS.gene67583.t1 MTR_5g023530 87.805 287 34 1 1 286 1 287 2.26e-175 485
MS.gene67583.t1 MTR_5g023520 88.421 285 31 2 1 284 1 284 4.57e-175 484
MS.gene67583.t1 MTR_5g023320 87.719 285 31 2 1 284 1 282 6.52e-175 484
MS.gene67583.t1 MTR_5g023470 86.760 287 37 1 1 286 1 287 5.08e-173 479
MS.gene67583.t1 MTR_5g023580 84.615 286 40 2 1 282 1 286 7.66e-166 464
MS.gene67583.t1 MTR_8g074450 67.944 287 86 2 4 284 3 289 1.14e-135 385
MS.gene67583.t1 MTR_8g074420 67.361 288 88 2 3 284 2 289 6.15e-131 373
MS.gene67583.t1 MTR_8g074510 65.614 285 90 3 6 284 5 287 3.45e-130 371
MS.gene67583.t1 MTR_5g023460 85.405 185 23 1 100 284 11 191 1.52e-109 315
MS.gene67583.t1 MTR_8g074530 43.793 290 153 5 3 284 17 304 6.31e-66 208
MS.gene67583.t1 MTR_5g023290 36.667 300 172 5 3 284 9 308 1.12e-54 179
MS.gene67583.t1 MTR_5g023410 90.816 98 9 0 3 100 2 99 1.07e-51 169
MS.gene67583.t1 MTR_5g023650 36.301 292 170 4 8 284 14 304 1.04e-48 164
MS.gene67583.t1 MTR_8g074380 35.374 294 164 6 1 285 7 283 2.02e-46 159
MS.gene67583.t1 MTR_3g116950 37.024 289 167 9 5 283 11 294 2.65e-44 152
MS.gene67583.t1 MTR_4g070530 33.333 282 183 3 7 283 13 294 4.19e-43 149
MS.gene67583.t1 MTR_4g070590 35.836 293 173 8 3 283 10 299 1.10e-39 140
MS.gene67583.t1 MTR_3g116920 34.828 290 174 8 5 283 11 296 2.77e-38 137
MS.gene67583.t1 MTR_4g070570 29.492 295 191 7 2 283 11 301 5.16e-38 136
MS.gene67583.t1 MTR_4g070540 32.862 283 183 4 7 283 13 294 1.08e-37 135
MS.gene67583.t1 MTR_3g115150 28.618 304 181 10 6 286 27 317 1.63e-24 100
MS.gene67583.t1 MTR_1g037820 30.769 234 144 5 52 284 9 225 4.61e-24 97.8
MS.gene67583.t1 MTR_5g023540 54.412 68 28 1 164 231 2 66 1.35e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene67583.t1 AT4G35690 38.028 284 161 7 7 284 9 283 3.94e-54 177
MS.gene67583.t1 AT2G17680 38.176 296 152 10 7 284 9 291 5.07e-48 162
MS.gene67583.t1 AT2G17080 36.786 280 154 6 5 283 4 261 5.09e-47 158
MS.gene67583.t1 AT2G17070 35.971 278 155 6 5 281 4 259 3.07e-43 149
MS.gene67583.t1 AT4G35710 35.640 289 160 7 7 284 9 282 1.16e-42 147
MS.gene67583.t1 AT4G35200 34.050 279 154 7 5 282 4 253 4.24e-41 143
MS.gene67583.t1 AT4G35210 32.975 279 154 7 5 282 4 250 2.91e-38 135
MS.gene67583.t1 AT4G35720 30.990 313 180 5 6 284 14 324 7.28e-28 110
MS.gene67583.t1 AT4G35680 31.667 300 174 9 8 284 18 309 1.27e-27 108
MS.gene67583.t1 AT1G76240 28.571 294 182 7 5 284 28 307 4.95e-24 99.4
MS.gene67583.t1 AT4G35660 29.470 302 174 8 1 285 9 288 2.86e-23 97.1
MS.gene67583.t1 AT3G51400 31.633 294 166 10 5 285 4 275 1.03e-20 89.7
MS.gene67583.t1 AT1G76210 29.130 230 120 6 68 284 26 225 1.06e-17 80.5
MS.gene67583.t1 AT3G51410 31.429 280 150 9 8 284 39 279 7.05e-15 73.6
MS.gene67583.t1 AT1G20520 27.602 221 127 6 72 284 30 225 1.69e-13 68.9
MS.gene67583.t1 AT1G76220 29.798 198 112 6 2 191 5 183 7.11e-12 64.7

Find 48 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACATCATGACTCTATCTT+AGG 0.153901 5.2:+14218214 MS.gene67583:CDS
AAACATATGGAAACTAAATT+TGG 0.157953 5.2:+14217825 MS.gene67583:CDS
CATTAGAACCTTCACTTATA+AGG 0.159224 5.2:-14218088 None:intergenic
GGCTTCCCATGTCTTCATTT+TGG 0.197889 5.2:-14217460 None:intergenic
GCAATTGAAATGATAGAAAA+TGG 0.225171 5.2:+14218143 MS.gene67583:CDS
TGAAGATTTGTATATTTCTT+TGG 0.280108 5.2:+14217530 MS.gene67583:CDS
TTCTTCAATTCTTGTGGAGT+TGG 0.333799 5.2:-14217430 None:intergenic
AGAGAATATATTTAGGTGTT+TGG 0.341972 5.2:+14218169 MS.gene67583:CDS
ATTAGAACCTTCACTTATAA+GGG 0.348108 5.2:-14218087 None:intergenic
TCTTCAATTCTTGTGGAGTT+GGG 0.358306 5.2:-14217429 None:intergenic
GAAATTTGTGGAAGAGTTGT+TGG 0.365610 5.2:+14217608 MS.gene67583:CDS
GATCACATCTTTAAAACATA+TGG 0.374642 5.2:+14217812 MS.gene67583:CDS
CACTTCACTCTGCTCTTAGA+AGG 0.400152 5.2:+14217706 MS.gene67583:CDS
CAAAGTCAAAGGCAACAAAA+TGG 0.408302 5.2:+14217970 MS.gene67583:CDS
ATGGTTTAGAGAATATATTT+AGG 0.411004 5.2:+14218162 MS.gene67583:CDS
ACATGAAAGATTTGAGGCTT+TGG 0.411831 5.2:+14218115 MS.gene67583:CDS
TAGCTCTTCTTCAATTCTTG+TGG 0.429253 5.2:-14217436 None:intergenic
TGTTGCACATGAAAGATTTG+AGG 0.445316 5.2:+14218109 MS.gene67583:CDS
GCGAACATGGTAATTGTTGC+TGG 0.461700 5.2:-14217379 None:intergenic
TAGATATATGCGGCATTACA+AGG 0.466485 5.2:+14217649 MS.gene67583:CDS
CTCTGCTCTTAGAAGGAGAA+AGG 0.467785 5.2:+14217713 MS.gene67583:CDS
CCATCATGGTGAGAAATTTG+TGG 0.469148 5.2:+14217596 MS.gene67583:CDS
AACATCATGACTCTATCTTA+GGG 0.475458 5.2:+14218215 MS.gene67583:CDS
AATATACAAATCTTCAAGAC+AGG 0.496819 5.2:-14217523 None:intergenic
TGACAAAGTTGATGCAAAAG+AGG 0.510512 5.2:+14218000 MS.gene67583:CDS
TTAAGAACCCTTATAAGTGA+AGG 0.516729 5.2:+14218080 MS.gene67583:CDS
CCACAAATTTCTCACCATGA+TGG 0.539683 5.2:-14217596 None:intergenic
AAGCTTGTTTCTCACCATCA+TGG 0.543029 5.2:+14217582 MS.gene67583:CDS
GAAAACTGTTTGAGCGAACA+TGG 0.549885 5.2:-14217392 None:intergenic
AGCTAACCAAAATGAAGACA+TGG 0.554329 5.2:+14217454 MS.gene67583:CDS
GGTATCATGTGAAGAGAACA+TGG 0.559553 5.2:+14218022 MS.gene67583:CDS
TCTGCTCTTAGAAGGAGAAA+GGG 0.566127 5.2:+14217714 MS.gene67583:CDS
ACATCTGATTCAATTACCAC+TGG 0.584960 5.2:+14217495 MS.gene67583:CDS
TGGATCTTCATCAAAGTCAA+AGG 0.588491 5.2:+14217959 MS.gene67583:CDS
GACAAAGTTGATGCAAAAGA+GGG 0.591207 5.2:+14218001 MS.gene67583:CDS
ATGAAAATGTTAGATATATG+CGG 0.607340 5.2:+14217639 MS.gene67583:CDS
ATCAGATGTGGATGTTGATG+TGG 0.611639 5.2:-14217481 None:intergenic
TTTGTGGAAGAGTTGTTGGA+TGG 0.615570 5.2:+14217612 MS.gene67583:CDS
TTGATCTTGATTCGAAAGTG+AGG 0.621821 5.2:-14217853 None:intergenic
AGTGATTACAGTTCTTAGAG+AGG 0.623623 5.2:+14217887 MS.gene67583:CDS
ACAAATCTTCAAGACAGGAA+AGG 0.623985 5.2:-14217518 None:intergenic
AGATATATGCGGCATTACAA+GGG 0.630608 5.2:+14217650 MS.gene67583:CDS
GCTAACCAAAATGAAGACAT+GGG 0.637923 5.2:+14217455 MS.gene67583:CDS
CTTCAATGAACTGCAGTGTG+TGG 0.642952 5.2:+14218049 MS.gene67583:CDS
GAACTGTAATCACTGCAACA+AGG 0.656672 5.2:-14217878 None:intergenic
AGTGGTAATTGAATCAGATG+TGG 0.659124 5.2:-14217493 None:intergenic
TTCAAGACAGGAAAGGCCAG+TGG 0.676275 5.2:-14217511 None:intergenic
GAGTTGGGATGAGATTGAGA+AGG 0.749032 5.2:-14217414 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCTTTAATTTGTAACA+TGG - chr5.2:14217679-14217698 None:intergenic 15.0%
!! AAACATATGGAAACTAAATT+TGG + chr5.2:14217825-14217844 MS.gene67583:CDS 20.0%
!! ATGAAAATGTTAGATATATG+CGG + chr5.2:14217639-14217658 MS.gene67583:CDS 20.0%
!! ATGGTTTAGAGAATATATTT+AGG + chr5.2:14218162-14218181 MS.gene67583:CDS 20.0%
!! TGAAGATTTGTATATTTCTT+TGG + chr5.2:14217530-14217549 MS.gene67583:CDS 20.0%
! AATATACAAATCTTCAAGAC+AGG - chr5.2:14217526-14217545 None:intergenic 25.0%
! AGAGAATATATTTAGGTGTT+TGG + chr5.2:14218169-14218188 MS.gene67583:CDS 25.0%
! ATTAGAACCTTCACTTATAA+GGG - chr5.2:14218090-14218109 None:intergenic 25.0%
! GCAATTGAAATGATAGAAAA+TGG + chr5.2:14218143-14218162 MS.gene67583:CDS 25.0%
!!! GATCACATCTTTAAAACATA+TGG + chr5.2:14217812-14217831 MS.gene67583:CDS 25.0%
CATTAGAACCTTCACTTATA+AGG - chr5.2:14218091-14218110 None:intergenic 30.0%
TTAAGAACCCTTATAAGTGA+AGG + chr5.2:14218080-14218099 MS.gene67583:CDS 30.0%
!! TCAATCAGTTTTGTCTTTCT+TGG + chr5.2:14217935-14217954 MS.gene67583:CDS 30.0%
ACAAATCTTCAAGACAGGAA+AGG - chr5.2:14217521-14217540 None:intergenic 35.0%
ACATCTGATTCAATTACCAC+TGG + chr5.2:14217495-14217514 MS.gene67583:CDS 35.0%
AGATATATGCGGCATTACAA+GGG + chr5.2:14217650-14217669 MS.gene67583:CDS 35.0%
AGCTAACCAAAATGAAGACA+TGG + chr5.2:14217454-14217473 MS.gene67583:CDS 35.0%
AGTGATTACAGTTCTTAGAG+AGG + chr5.2:14217887-14217906 MS.gene67583:CDS 35.0%
AGTGGTAATTGAATCAGATG+TGG - chr5.2:14217496-14217515 None:intergenic 35.0%
CAAAGTCAAAGGCAACAAAA+TGG + chr5.2:14217970-14217989 MS.gene67583:CDS 35.0%
GAAATTTGTGGAAGAGTTGT+TGG + chr5.2:14217608-14217627 MS.gene67583:CDS 35.0%
GCTAACCAAAATGAAGACAT+GGG + chr5.2:14217455-14217474 MS.gene67583:CDS 35.0%
TAGATATATGCGGCATTACA+AGG + chr5.2:14217649-14217668 MS.gene67583:CDS 35.0%
TAGCTCTTCTTCAATTCTTG+TGG - chr5.2:14217439-14217458 None:intergenic 35.0%
TCTTCAATTCTTGTGGAGTT+GGG - chr5.2:14217432-14217451 None:intergenic 35.0%
TGGATCTTCATCAAAGTCAA+AGG + chr5.2:14217959-14217978 MS.gene67583:CDS 35.0%
TGTTGCACATGAAAGATTTG+AGG + chr5.2:14218109-14218128 MS.gene67583:CDS 35.0%
TTCTTCAATTCTTGTGGAGT+TGG - chr5.2:14217433-14217452 None:intergenic 35.0%
! ACATGAAAGATTTGAGGCTT+TGG + chr5.2:14218115-14218134 MS.gene67583:CDS 35.0%
! TTGATCTTGATTCGAAAGTG+AGG - chr5.2:14217856-14217875 None:intergenic 35.0%
!! GACAAAGTTGATGCAAAAGA+GGG + chr5.2:14218001-14218020 MS.gene67583:CDS 35.0%
!! TCAGTTTTGTCTTTCTTGGT+TGG + chr5.2:14217939-14217958 MS.gene67583:CDS 35.0%
!! TGACAAAGTTGATGCAAAAG+AGG + chr5.2:14218000-14218019 MS.gene67583:CDS 35.0%
ATCAGATGTGGATGTTGATG+TGG - chr5.2:14217484-14217503 None:intergenic 40.0%
CCACAAATTTCTCACCATGA+TGG - chr5.2:14217599-14217618 None:intergenic 40.0%
CCATCATGGTGAGAAATTTG+TGG + chr5.2:14217596-14217615 MS.gene67583:CDS 40.0%
GAAAACTGTTTGAGCGAACA+TGG - chr5.2:14217395-14217414 None:intergenic 40.0%
GAACTGTAATCACTGCAACA+AGG - chr5.2:14217881-14217900 None:intergenic 40.0%
GGTATCATGTGAAGAGAACA+TGG + chr5.2:14218022-14218041 MS.gene67583:CDS 40.0%
TCTGCTCTTAGAAGGAGAAA+GGG + chr5.2:14217714-14217733 MS.gene67583:CDS 40.0%
TTTGTGGAAGAGTTGTTGGA+TGG + chr5.2:14217612-14217631 MS.gene67583:CDS 40.0%
! AAGCTTGTTTCTCACCATCA+TGG + chr5.2:14217582-14217601 MS.gene67583:CDS 40.0%
CACTTCACTCTGCTCTTAGA+AGG + chr5.2:14217706-14217725 MS.gene67583:CDS 45.0%
CTCTGCTCTTAGAAGGAGAA+AGG + chr5.2:14217713-14217732 MS.gene67583:CDS 45.0%
CTTCAATGAACTGCAGTGTG+TGG + chr5.2:14218049-14218068 MS.gene67583:CDS 45.0%
GAGTTGGGATGAGATTGAGA+AGG - chr5.2:14217417-14217436 None:intergenic 45.0%
GCGAACATGGTAATTGTTGC+TGG - chr5.2:14217382-14217401 None:intergenic 45.0%
! GGCTTCCCATGTCTTCATTT+TGG - chr5.2:14217463-14217482 None:intergenic 45.0%
TTCAAGACAGGAAAGGCCAG+TGG - chr5.2:14217514-14217533 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 14217375 14218235 14217375 ID=MS.gene67583
chr5.2 mRNA 14217375 14218235 14217375 ID=MS.gene67583.t1;Parent=MS.gene67583
chr5.2 exon 14217375 14218235 14217375 ID=MS.gene67583.t1.exon1;Parent=MS.gene67583.t1
chr5.2 CDS 14217375 14218235 14217375 ID=cds.MS.gene67583.t1;Parent=MS.gene67583.t1
Gene Sequence

>MS.gene67583

ATGGCCAGCAACAATTACCATGTTCGCTCAAACAGTTTTCCTTCTCAATCTCATCCCAACTCCACAAGAATTGAAGAAGAGCTAACCAAAATGAAGACATGGGAAGCCACATCAACATCCACATCTGATTCAATTACCACTGGCCTTTCCTGTCTTGAAGATTTGTATATTTCTTTGGAAGATCTTCTCAATATGTCATCAACACAGAAGCTTGTTTCTCACCATCATGGTGAGAAATTTGTGGAAGAGTTGTTGGATGGTTCAATGAAAATGTTAGATATATGCGGCATTACAAGGGATGCCATGTTACAAATTAAAGAAAATGTTCAATCACTTCACTCTGCTCTTAGAAGGAGAAAGGGAGATTCAAGCATTGAAAAAAGTGTAGCAGAATATAAATTTTTCACAAAGAAGATGAAGAAGAATGTCACAAAGTTGATCACATCTTTAAAACATATGGAAACTAAATTTGGAGCTTCCTCACTTTCGAATCAAGATCAAGACCTTGTTGCAGTGATTACAGTTCTTAGAGAGGTCATTAAAATGAACTTGTCTATCTTTCAATCAGTTTTGTCTTTCTTGGTTGGATCTTCATCAAAGTCAAAGGCAACAAAATGGTTGAAAGTGACAAAGTTGATGCAAAAGAGGGTATCATGTGAAGAGAACATGGAGAACTTCAATGAACTGCAGTGTGTGGAAGCATCTTTAAGAACCCTTATAAGTGAAGGTTCTAATGTTGCACATGAAAGATTTGAGGCTTTGGAGAATGCAATTGAAATGATAGAAAATGGTTTAGAGAATATATTTAGGTGTTTGGTTAAAACTAGAGTCTGTCTTTTGAACATCATGACTCTATCTTAG
Protein sequence

>MS.gene67583.t1

MASNNYHVRSNSFPSQSHPNSTRIEEELTKMKTWEATSTSTSDSITTGLSCLEDLYISLEDLLNMSSTQKLVSHHHGEKFVEELLDGSMKMLDICGITRDAMLQIKENVQSLHSALRRRKGDSSIEKSVAEYKFFTKKMKKNVTKLITSLKHMETKFGASSLSNQDQDLVAVITVLREVIKMNLSIFQSVLSFLVGSSSKSKATKWLKVTKLMQKRVSCEENMENFNELQCVEASLRTLISEGSNVAHERFEALENAIEMIENGLENIFRCLVKTRVCLLNIMTLS