Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95250.t1 | XP_013445069.1 | 95.7 | 1175 | 51 | 0 | 1 | 1175 | 1 | 1175 | 0.00E+00 | 1583.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95250.t1 | Q9SN90 | 77.1 | 1174 | 266 | 1 | 1 | 1174 | 1 | 1171 | 0.0e+00 | 1243.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95250.t1 | A0A072TZX1 | 95.7 | 1175 | 51 | 0 | 1 | 1175 | 1 | 1175 | 0.0e+00 | 1583.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050297 | MS.gene95250 | 0.802027 | 6.82E-49 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95250.t1 | MTR_8g037325 | 97.617 | 1175 | 28 | 0 | 1 | 1175 | 1 | 1175 | 0.0 | 2330 |
| MS.gene95250.t1 | MTR_1g051825 | 24.425 | 1261 | 780 | 44 | 1 | 1166 | 25 | 1207 | 6.68e-68 | 250 |
| MS.gene95250.t1 | MTR_1g051825 | 24.425 | 1261 | 780 | 44 | 1 | 1166 | 1 | 1183 | 8.58e-68 | 250 |
| MS.gene95250.t1 | MTR_8g072890 | 25.079 | 1268 | 761 | 50 | 1 | 1154 | 14 | 1206 | 5.34e-50 | 194 |
| MS.gene95250.t1 | MTR_0003s0460 | 22.624 | 1273 | 769 | 44 | 3 | 1154 | 6 | 1183 | 3.94e-36 | 149 |
| MS.gene95250.t1 | MTR_7g091420 | 25.768 | 423 | 239 | 14 | 1 | 400 | 9 | 379 | 9.04e-20 | 93.6 |
| MS.gene95250.t1 | MTR_7g091430 | 26.792 | 265 | 169 | 6 | 903 | 1154 | 572 | 824 | 3.37e-16 | 84.3 |
| MS.gene95250.t1 | MTR_1g069910 | 22.131 | 732 | 414 | 28 | 520 | 1154 | 519 | 1191 | 7.39e-13 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95250.t1 | AT5G62410 | 77.021 | 1175 | 270 | 0 | 1 | 1175 | 1 | 1175 | 0.0 | 1796 |
| MS.gene95250.t1 | AT3G47460 | 75.554 | 1174 | 287 | 0 | 1 | 1174 | 1 | 1174 | 0.0 | 1790 |
| MS.gene95250.t1 | AT3G47460 | 75.383 | 1174 | 286 | 1 | 1 | 1174 | 1 | 1171 | 0.0 | 1780 |
| MS.gene95250.t1 | AT2G27170 | 24.803 | 1270 | 765 | 40 | 1 | 1166 | 1 | 1184 | 1.93e-61 | 230 |
| MS.gene95250.t1 | AT2G27170 | 24.803 | 1270 | 765 | 40 | 1 | 1166 | 1 | 1184 | 1.93e-61 | 230 |
| MS.gene95250.t1 | AT2G27170 | 24.407 | 1139 | 682 | 39 | 1 | 1054 | 1 | 1045 | 1.26e-46 | 183 |
| MS.gene95250.t1 | AT2G27170 | 24.407 | 1139 | 682 | 39 | 1 | 1054 | 1 | 1045 | 1.26e-46 | 183 |
| MS.gene95250.t1 | AT5G48600 | 23.753 | 1263 | 781 | 46 | 1 | 1154 | 24 | 1213 | 1.67e-41 | 167 |
| MS.gene95250.t1 | AT5G48600 | 29.268 | 164 | 107 | 3 | 996 | 1154 | 1057 | 1216 | 3.87e-17 | 87.8 |
| MS.gene95250.t1 | AT3G54670 | 20.765 | 366 | 233 | 8 | 834 | 1154 | 840 | 1193 | 1.61e-12 | 72.8 |
Find 220 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGCACTTTCTCTGATTC+TGG | 0.169360 | 8.4:-60620120 | MS.gene95250:CDS |
| GATTCCATTTGCTTCGTTCT+TGG | 0.197936 | 8.4:-60628686 | MS.gene95250:CDS |
| AGGAACAAGTATTTGATAAA+TGG | 0.209184 | 8.4:-60627191 | MS.gene95250:CDS |
| CTTCTCGCAATGGCTAATTT+TGG | 0.232238 | 8.4:+60626016 | None:intergenic |
| AGCAGTCAACTGAGCTATTT+TGG | 0.235151 | 8.4:+60626689 | None:intergenic |
| ACGTTTCCTTCACAAGATTC+TGG | 0.239221 | 8.4:+60626604 | None:intergenic |
| GGAAAACAGCTACTACAAAA+TGG | 0.240659 | 8.4:-60625425 | MS.gene95250:CDS |
| TGCCGAAATTGCACTTTCTT+TGG | 0.256016 | 8.4:-60625198 | MS.gene95250:CDS |
| CGGTAATAGTGTAGAAAATA+TGG | 0.311541 | 8.4:+60620268 | None:intergenic |
| TCAAGAAAGCTGAATCCTTC+TGG | 0.316791 | 8.4:+60620221 | None:intergenic |
| CTTTGACCTATCAAAGTTCT+TGG | 0.320800 | 8.4:+60625640 | None:intergenic |
| GCAACACGCACCGTTGTTCC+TGG | 0.323171 | 8.4:-60628770 | MS.gene95250:CDS |
| AATCGAGAAAGTAGGCTAAA+AGG | 0.327465 | 8.4:-60622727 | MS.gene95250:CDS |
| AACCAAAGAAAGTGCAATTT+CGG | 0.330095 | 8.4:+60625196 | None:intergenic |
| TTCCAGCTTTGGAGAAGTTA+AGG | 0.343317 | 8.4:-60626914 | MS.gene95250:CDS |
| TATTGTTCCTTTAGTGACTT+TGG | 0.344973 | 8.4:-60620293 | MS.gene95250:intron |
| GTCATCAATCTCAGTTATCT+TGG | 0.345282 | 8.4:+60626752 | None:intergenic |
| GAAGGCCTCTGCCATTAAAA+AGG | 0.348915 | 8.4:-60626340 | MS.gene95250:CDS |
| CACAATTGAAACAGTAGCTT+TGG | 0.355453 | 8.4:+60628589 | None:intergenic |
| CTTGAGGTTAAAGTTGCATT+TGG | 0.356297 | 8.4:-60620194 | MS.gene95250:CDS |
| CGCAATCAAGGCTTGAGAAA+AGG | 0.357272 | 8.4:-60620939 | MS.gene95250:intron |
| CAGCCAAGTGGTCTTCTGAC+TGG | 0.360379 | 8.4:-60623855 | MS.gene95250:CDS |
| AATGCAATGGAATATGTCTT+TGG | 0.367641 | 8.4:-60624900 | MS.gene95250:CDS |
| GGAACAAGTATTTGATAAAT+GGG | 0.377570 | 8.4:-60627190 | MS.gene95250:CDS |
| ATACACAAGCTCTTGAAGAT+TGG | 0.378596 | 8.4:+60628634 | None:intergenic |
| CGTTTCCTTCACAAGATTCT+GGG | 0.383248 | 8.4:+60626605 | None:intergenic |
| TCAGGAGATACTTCCAGCTT+TGG | 0.383477 | 8.4:-60626925 | MS.gene95250:CDS |
| ATTACCGGTTTAAACGGTTC+TGG | 0.383578 | 8.4:-60628725 | MS.gene95250:CDS |
| GAAGGATTCAGCTTTCTTGA+TGG | 0.383695 | 8.4:-60620218 | MS.gene95250:CDS |
| AAGCTTCAGGCTGAACAATC+AGG | 0.383967 | 8.4:-60621068 | MS.gene95250:CDS |
| ACCTGCTCCACTTTACATCT+TGG | 0.384395 | 8.4:-60620081 | MS.gene95250:CDS |
| AGCCAAGTGGTCTTCTGACT+GGG | 0.390511 | 8.4:-60623854 | MS.gene95250:CDS |
| GGAGCATGAAAAGGAATATC+AGG | 0.392844 | 8.4:-60626259 | MS.gene95250:intron |
| TCCTTACAAAGAGGGTGAAA+TGG | 0.395557 | 8.4:-60625874 | MS.gene95250:CDS |
| TATCCAGGAGCAAGCTTCCT+TGG | 0.399878 | 8.4:-60621842 | MS.gene95250:CDS |
| AGAAAGTAGGCTAAAAGGTT+TGG | 0.400704 | 8.4:-60622722 | MS.gene95250:CDS |
| ACAACGAACCTGTGTCAAAT+TGG | 0.403796 | 8.4:+60628658 | None:intergenic |
| TTCCTTAACTTCTCCAAAGC+TGG | 0.407122 | 8.4:+60626912 | None:intergenic |
| TATGGTGGATGTTAAAGAAA+TGG | 0.409848 | 8.4:-60626715 | MS.gene95250:CDS |
| TCAACAAGCTGCTGTTAGAT+TGG | 0.411671 | 8.4:-60625333 | MS.gene95250:intron |
| TCCATTTCACCCTCTTTGTA+AGG | 0.412254 | 8.4:+60625873 | None:intergenic |
| GATGTTGAAAATATCAAAAC+AGG | 0.415509 | 8.4:-60625909 | MS.gene95250:CDS |
| AACGCAATTACCGGTTTAAA+CGG | 0.415856 | 8.4:-60628731 | MS.gene95250:CDS |
| GGAAGAACTCGAGAAGCTTC+AGG | 0.419712 | 8.4:-60621081 | MS.gene95250:CDS |
| CCAGGTAAAACGAATGGAAA+TGG | 0.423754 | 8.4:-60621234 | MS.gene95250:intron |
| TAATCCTCCCTTGCATTATG+AGG | 0.425595 | 8.4:+60627096 | None:intergenic |
| AAACAGTTGTTTGGTAAAAG+TGG | 0.429212 | 8.4:-60621146 | MS.gene95250:CDS |
| GATCAACAAGCTTCTTGATC+AGG | 0.430039 | 8.4:-60626943 | MS.gene95250:CDS |
| CTGAAACAATGAAAGATCAT+AGG | 0.433798 | 8.4:+60623032 | None:intergenic |
| TGAAATTACCGTTACTAGAC+AGG | 0.435488 | 8.4:-60628516 | MS.gene95250:intron |
| GGAGAGGCTTGTAATGGAAA+TGG | 0.436064 | 8.4:-60621872 | MS.gene95250:CDS |
| AAGTTGCATTTGGAAGTGTA+TGG | 0.438259 | 8.4:-60620184 | MS.gene95250:CDS |
| GAAAATGAAGCAGTGTGATA+AGG | 0.445351 | 8.4:-60621614 | MS.gene95250:CDS |
| TGTGTTTGATAATTCTGAAA+GGG | 0.445875 | 8.4:-60628570 | MS.gene95250:CDS |
| AGAGTAATCTTGAAAGGAAG+AGG | 0.446630 | 8.4:-60621540 | MS.gene95250:CDS |
| GACCTATCAAAGTTCTTGGC+AGG | 0.447396 | 8.4:+60625644 | None:intergenic |
| AACTCTTGAGGGAACAGTAT+AGG | 0.451920 | 8.4:+60625355 | None:intergenic |
| AGAATCAGTTAAAGTCAAAA+CGG | 0.455138 | 8.4:-60625977 | MS.gene95250:CDS |
| TGATTTGAAAAGTAGATTTG+AGG | 0.457698 | 8.4:-60626301 | MS.gene95250:CDS |
| GCAATCAAGGCTTGAGAAAA+GGG | 0.458181 | 8.4:-60620938 | MS.gene95250:intron |
| TCAAGCATAGACAATATCTC+TGG | 0.460101 | 8.4:+60627050 | None:intergenic |
| AATGAAATCTCTGTCGGATA+AGG | 0.460698 | 8.4:-60626640 | MS.gene95250:CDS |
| GTTTATTGTGGTGTCACTAA+AGG | 0.462324 | 8.4:-60618185 | MS.gene95250:CDS |
| CAGCTTTCTTGATGGTCTTG+AGG | 0.462965 | 8.4:-60620210 | MS.gene95250:CDS |
| GAAAACAGCTACTACAAAAT+GGG | 0.468364 | 8.4:-60625424 | MS.gene95250:CDS |
| GCAGCTTGTTGAACTCTTGA+GGG | 0.469446 | 8.4:+60625344 | None:intergenic |
| TGAAAATATCAAAACAGGAT+TGG | 0.469652 | 8.4:-60625904 | MS.gene95250:CDS |
| CAACGAGGAGAAATGCTTAG+AGG | 0.470079 | 8.4:-60626102 | MS.gene95250:CDS |
| TTAGTTGTACGGAATGGAAA+AGG | 0.480194 | 8.4:+60627138 | None:intergenic |
| GAATCAGTTAAAGTCAAAAC+GGG | 0.481292 | 8.4:-60625976 | MS.gene95250:CDS |
| TCTCCAAGGAAGCTTGCTCC+TGG | 0.485034 | 8.4:+60621839 | None:intergenic |
| TTTGCCAGAACCGTTTAAAC+CGG | 0.485861 | 8.4:+60628721 | None:intergenic |
| GTCATCTTCAAAAGACCTAA+AGG | 0.486816 | 8.4:-60622665 | MS.gene95250:intron |
| ATTGTTCACATTTAGTTGTA+CGG | 0.488274 | 8.4:+60627127 | None:intergenic |
| TGCTGAGAAGGAAGCAAGCA+TGG | 0.488461 | 8.4:-60626670 | MS.gene95250:CDS |
| TCTAACTTGGCCATTGTGCC+CGG | 0.489559 | 8.4:+60620248 | None:intergenic |
| GATTCCAAGAACGAAGCAAA+TGG | 0.490395 | 8.4:+60628682 | None:intergenic |
| GAAATTGCACTTTCTTTGGT+TGG | 0.490717 | 8.4:-60625194 | MS.gene95250:CDS |
| TCCTTCTGGAGGTTCTAACT+TGG | 0.491158 | 8.4:+60620235 | None:intergenic |
| AACTCATCAAAGTGAAAGAT+AGG | 0.492152 | 8.4:-60625598 | MS.gene95250:CDS |
| TGGTTATCAAGAAGAATTGA+AGG | 0.495383 | 8.4:-60625174 | MS.gene95250:intron |
| TAATCTTGAAAGGAAGAGGA+TGG | 0.495805 | 8.4:-60621536 | MS.gene95250:CDS |
| TTTGTTCCAGGTAAAACGAA+TGG | 0.496337 | 8.4:-60621240 | MS.gene95250:intron |
| CTGATAGGTGGCATTTAGTC+GGG | 0.497381 | 8.4:-60623919 | MS.gene95250:intron |
| ACGCAATCTCTCAGCATACT+TGG | 0.498016 | 8.4:-60625693 | MS.gene95250:CDS |
| TGGAGAAGTTAAGGAAAGAA+AGG | 0.499569 | 8.4:-60626905 | MS.gene95250:CDS |
| TTACGTGTTCACTTTGGTCC+AGG | 0.500889 | 8.4:+60620692 | None:intergenic |
| TCTTGAGAAGAAGCAGAGTA+AGG | 0.501074 | 8.4:-60626973 | MS.gene95250:CDS |
| TGAAAAGGAGAGGCTTGTAA+TGG | 0.501869 | 8.4:-60621878 | MS.gene95250:CDS |
| GTTATCAGAGATTGAGGCAA+AGG | 0.502725 | 8.4:-60623492 | MS.gene95250:intron |
| CTTTCAGTTCCTTCTCGCAA+TGG | 0.507756 | 8.4:+60626006 | None:intergenic |
| ACCTCGTCCAAGATGTAAAG+TGG | 0.509623 | 8.4:+60620074 | None:intergenic |
| ATCCTGCCAAGAACTTTGAT+AGG | 0.510683 | 8.4:-60625646 | MS.gene95250:CDS |
| TGAAATGGAAGCTTTGCAAA+AGG | 0.511355 | 8.4:-60625859 | MS.gene95250:intron |
| GAACCTGGAGGAGCATGAAA+AGG | 0.518225 | 8.4:-60626268 | MS.gene95250:CDS |
| ATTGTAACGCTGAGTTAGAT+AGG | 0.518519 | 8.4:-60626860 | MS.gene95250:CDS |
| GGTGATGCAAAGATAGCAGT+TGG | 0.519540 | 8.4:-60626071 | MS.gene95250:CDS |
| TCCAAGATGTAAAGTGGAGC+AGG | 0.519656 | 8.4:+60620080 | None:intergenic |
| AGCAGCTTGTTGAACTCTTG+AGG | 0.520161 | 8.4:+60625343 | None:intergenic |
| AATTTCATTGCAGGTGGGCA+AGG | 0.521336 | 8.4:-60625225 | MS.gene95250:intron |
| GAAAGGGGTCGGAGTCCGCT+TGG | 0.522247 | 8.4:-60628554 | MS.gene95250:CDS |
| TCACATTTAGTTGTACGGAA+TGG | 0.529589 | 8.4:+60627132 | None:intergenic |
| GGAAATGGAAGCTGTTATCC+AGG | 0.529804 | 8.4:-60621857 | MS.gene95250:CDS |
| AATGCAAGGGAGGATTACTA+AGG | 0.531218 | 8.4:-60627090 | MS.gene95250:CDS |
| ATAAACTTACGTGTTCACTT+TGG | 0.531279 | 8.4:+60620686 | None:intergenic |
| AGCTCAGTTGACTGCTGAGA+AGG | 0.531731 | 8.4:-60626682 | MS.gene95250:CDS |
| AACAGTTGTTTGGTAAAAGT+GGG | 0.531898 | 8.4:-60621145 | MS.gene95250:CDS |
| TTAGGGACATGATAATGAAA+AGG | 0.532136 | 8.4:-60621893 | MS.gene95250:intron |
| AAAATGAGCTCAAAGCTAGA+AGG | 0.533492 | 8.4:-60625935 | MS.gene95250:CDS |
| TGAGCAAAACGAGCATCATA+AGG | 0.534411 | 8.4:-60623001 | MS.gene95250:intron |
| CTTTGATAGGTCAAAGGTGA+AGG | 0.534957 | 8.4:-60625633 | MS.gene95250:CDS |
| GAAGCAATCTATAGAAGAGA+AGG | 0.535482 | 8.4:-60626358 | MS.gene95250:CDS |
| GTTGGTTATTACAGGGGAAG+CGG | 0.535512 | 8.4:-60623581 | MS.gene95250:intron |
| CGTAGGATTTGAATCCTTCG+AGG | 0.535880 | 8.4:+60628792 | None:intergenic |
| CAAGCATAGACAATATCTCT+GGG | 0.536394 | 8.4:+60627051 | None:intergenic |
| TTGATAATTCTGAAAGGGGT+CGG | 0.539419 | 8.4:-60628565 | MS.gene95250:CDS |
| TTGAGCCACACACAAAACAT+AGG | 0.541500 | 8.4:-60618544 | MS.gene95250:CDS |
| GTCTATGCTTGAGGAAGCTG+CGG | 0.541797 | 8.4:-60627039 | MS.gene95250:CDS |
| TGACATGGCTAAGACAACTA+TGG | 0.542943 | 8.4:-60626733 | MS.gene95250:CDS |
| GTGTATAAACAAGGTCAAGC+GGG | 0.543337 | 8.4:-60628617 | MS.gene95250:CDS |
| AAATCACTTTCCACACTCCC+AGG | 0.545421 | 8.4:-60618509 | MS.gene95250:intron |
| TCTGATAGGTGGCATTTAGT+CGG | 0.546853 | 8.4:-60623920 | MS.gene95250:intron |
| CAAAATTAGCCATTGCGAGA+AGG | 0.550598 | 8.4:-60626015 | MS.gene95250:CDS |
| ATGACAATAATCGAGAAAGT+AGG | 0.550766 | 8.4:-60622735 | MS.gene95250:CDS |
| TTCCGGACAAAATTTGTCGA+TGG | 0.551293 | 8.4:-60618133 | MS.gene95250:CDS |
| CTCGTGATCCTCGTAAAGCA+AGG | 0.552315 | 8.4:-60621103 | MS.gene95250:CDS |
| AAGGACGCAGTACATGCAAT+GGG | 0.553007 | 8.4:-60626886 | MS.gene95250:CDS |
| CCTTGCATTATGAGGAAATG+TGG | 0.554258 | 8.4:+60627104 | None:intergenic |
| GTCAACCTCTAGGGCAGTCA+CGG | 0.557478 | 8.4:+60625571 | None:intergenic |
| TTGTGTTTGATAATTCTGAA+AGG | 0.558341 | 8.4:-60628571 | MS.gene95250:CDS |
| CCAGGAGCGCGAATCTGAGA+GGG | 0.558584 | 8.4:-60625744 | MS.gene95250:intron |
| GAATCTCTTCCTTACAAAGA+GGG | 0.559901 | 8.4:-60625882 | MS.gene95250:CDS |
| GTAGTTGACACAGAAAGTAC+TGG | 0.560699 | 8.4:-60625446 | MS.gene95250:CDS |
| CCACATTTCCTCATAATGCA+AGG | 0.561004 | 8.4:-60627104 | MS.gene95250:CDS |
| AAGTAGGTTATCAGAGATTG+AGG | 0.562439 | 8.4:-60623498 | MS.gene95250:CDS |
| ATCAAGGAAATCTGCCTCGA+AGG | 0.564683 | 8.4:-60628806 | MS.gene95250:CDS |
| TGACCCTCTACCTGGGAGTG+TGG | 0.566185 | 8.4:+60618499 | None:intergenic |
| TGAGGAGCTCTCAAAGAACC+TGG | 0.567975 | 8.4:-60626283 | MS.gene95250:CDS |
| CAAGAGCTTGTGTATAAACA+AGG | 0.570748 | 8.4:-60628626 | MS.gene95250:CDS |
| AATTGATAGAGAAGCATGCA+TGG | 0.571327 | 8.4:-60621181 | MS.gene95250:CDS |
| TTAGTGAGAGTAATCTTGAA+AGG | 0.571501 | 8.4:-60621546 | MS.gene95250:CDS |
| GTGTTTGATAATTCTGAAAG+GGG | 0.572394 | 8.4:-60628569 | MS.gene95250:CDS |
| GGGGAAGCGGTGATCTATTG+AGG | 0.574600 | 8.4:-60623568 | MS.gene95250:CDS |
| AAAGGACGCAGTACATGCAA+TGG | 0.577163 | 8.4:-60626887 | MS.gene95250:CDS |
| AACTGTCGATGCTGCAAAGG+AGG | 0.578197 | 8.4:-60624859 | MS.gene95250:intron |
| TGATGCTAATTGATTCTCCA+AGG | 0.578693 | 8.4:+60621825 | None:intergenic |
| GGTGCAATGCAGATTGTGGT+TGG | 0.581648 | 8.4:-60627218 | MS.gene95250:intron |
| AGAGATATTGTCTATGCTTG+AGG | 0.583453 | 8.4:-60627048 | MS.gene95250:CDS |
| AGTTCTTCCCTTGCTTTACG+AGG | 0.583626 | 8.4:+60621095 | None:intergenic |
| GTATTTGTTCTAGAATGCAA+TGG | 0.584965 | 8.4:-60624913 | MS.gene95250:intron |
| CAAAACTGTCGATGCTGCAA+AGG | 0.585293 | 8.4:-60624862 | MS.gene95250:CDS |
| AGTAATGGCAATGTTTGAGA+AGG | 0.587695 | 8.4:-60620905 | MS.gene95250:CDS |
| GGGTTGTGTGCAAAAGTTGA+AGG | 0.590852 | 8.4:-60625723 | MS.gene95250:CDS |
| AGCTTCAGGCTGAACAATCA+GGG | 0.591274 | 8.4:-60621067 | MS.gene95250:intron |
| TGCAGATTGTGGTTGGAGGT+AGG | 0.592516 | 8.4:-60627211 | MS.gene95250:intron |
| AGACAATGCTATTTCTGAGG+TGG | 0.592664 | 8.4:-60626787 | MS.gene95250:CDS |
| TCTATGCTTGAGGAAGCTGC+GGG | 0.592726 | 8.4:-60627038 | MS.gene95250:CDS |
| GAAAGACTGTTCTGTGAGGG+TGG | 0.593114 | 8.4:-60621207 | MS.gene95250:CDS |
| CAAGAACTTTGATAGGTCAA+AGG | 0.593969 | 8.4:-60625639 | MS.gene95250:CDS |
| TGTCACGTTGTCAACCTCTA+GGG | 0.594997 | 8.4:+60625562 | None:intergenic |
| AACAACGGTGCGTGTTGCGT+AGG | 0.595679 | 8.4:+60628775 | None:intergenic |
| ACGGGATGAAGCTACGTCTG+TGG | 0.599890 | 8.4:-60625958 | MS.gene95250:CDS |
| AAGAGACGCTAAATGTTACC+TGG | 0.599939 | 8.4:-60620710 | MS.gene95250:CDS |
| GTGATGCAAAGATAGCAGTT+GGG | 0.602797 | 8.4:-60626070 | MS.gene95250:CDS |
| CGGAGAAATGAAATCTCTGT+CGG | 0.603080 | 8.4:-60626646 | MS.gene95250:CDS |
| CACATTTCCTCATAATGCAA+GGG | 0.604066 | 8.4:-60627103 | MS.gene95250:CDS |
| ATTGTGGTGTCACTAAAGGA+AGG | 0.604863 | 8.4:-60618181 | MS.gene95250:CDS |
| AAGGGTGAACAAGAAAGTAA+TGG | 0.605324 | 8.4:-60620920 | MS.gene95250:CDS |
| CTAACACACGATGTACATCA+AGG | 0.605499 | 8.4:-60628822 | None:intergenic |
| CAAAGACAATGCTATTTCTG+AGG | 0.606578 | 8.4:-60626790 | MS.gene95250:CDS |
| GGAATCTCTTCCTTACAAAG+AGG | 0.608720 | 8.4:-60625883 | MS.gene95250:CDS |
| TGAGCCACACACAAAACATA+GGG | 0.610273 | 8.4:-60618543 | MS.gene95250:CDS |
| GGCAATGTTTGAGAAGGCAG+AGG | 0.611737 | 8.4:-60620899 | MS.gene95250:CDS |
| TGGAATCACCAATTTGACAC+AGG | 0.612696 | 8.4:-60628666 | MS.gene95250:CDS |
| GCTGAGAAGGAAGCAAGCAT+GGG | 0.612728 | 8.4:-60626669 | MS.gene95250:CDS |
| TACACTATTACCGGGCACAA+TGG | 0.613373 | 8.4:-60620258 | MS.gene95250:CDS |
| AAAAGTTACCTGTCTAGTAA+CGG | 0.614719 | 8.4:+60628508 | None:intergenic |
| GATAACTGAGATTGATGACA+TGG | 0.621203 | 8.4:-60626748 | MS.gene95250:CDS |
| ACCACAAGTGTCACTCTCGA+AGG | 0.621839 | 8.4:-60623888 | MS.gene95250:CDS |
| CCAAGTGGTCTTCTGACTGG+GGG | 0.622001 | 8.4:-60623852 | MS.gene95250:CDS |
| GCCAAGTGGTCTTCTGACTG+GGG | 0.622632 | 8.4:-60623853 | MS.gene95250:CDS |
| GAGAAGGAAGCAAGCATGGG+CGG | 0.629277 | 8.4:-60626666 | MS.gene95250:CDS |
| TCGTGATCCTCGTAAAGCAA+GGG | 0.630792 | 8.4:-60621102 | MS.gene95250:CDS |
| GCAATGCAGATTGTGGTTGG+AGG | 0.632768 | 8.4:-60627215 | MS.gene95250:intron |
| GTCCACCGTGACTGCCCTAG+AGG | 0.633660 | 8.4:-60625576 | MS.gene95250:intron |
| ACTTTCCCAGAATCTTGTGA+AGG | 0.633794 | 8.4:-60626610 | MS.gene95250:CDS |
| ATTTCCTCATAATGCAAGGG+AGG | 0.634352 | 8.4:-60627100 | MS.gene95250:CDS |
| TGACAAGTCTAAGATCAAGA+AGG | 0.634890 | 8.4:-60620760 | MS.gene95250:CDS |
| TGTGTATAAACAAGGTCAAG+CGG | 0.636731 | 8.4:-60628618 | MS.gene95250:CDS |
| TGAGCTCAAAGCTAGAAGGA+AGG | 0.637698 | 8.4:-60625931 | MS.gene95250:CDS |
| GACATGATAATGAAAAGGAG+AGG | 0.639021 | 8.4:-60621888 | MS.gene95250:CDS |
| AAGGAAGAGGATGGAAAACG+AGG | 0.639227 | 8.4:-60621527 | MS.gene95250:intron |
| AGCATAGACAATATCTCTGG+GGG | 0.640180 | 8.4:+60627053 | None:intergenic |
| TCCACTTTACATCTTGGACG+AGG | 0.641213 | 8.4:-60620075 | MS.gene95250:intron |
| TCCAGGAGCGCGAATCTGAG+AGG | 0.643876 | 8.4:-60625745 | MS.gene95250:intron |
| ATAATAAAGAAGACACTCTG+AGG | 0.645280 | 8.4:-60626572 | MS.gene95250:CDS |
| ATTGAGGCAACTTCATGCTG+TGG | 0.645512 | 8.4:-60623552 | MS.gene95250:CDS |
| TGGCAAGAGCAGCGGCAACG+AGG | 0.651083 | 8.4:-60626117 | MS.gene95250:CDS |
| TTTGATAGGTCAAAGGTGAA+GGG | 0.652433 | 8.4:-60625632 | MS.gene95250:CDS |
| TCCTTCGAGAGTGACACTTG+TGG | 0.654927 | 8.4:+60623887 | None:intergenic |
| ACGAGAAGAGAAATCATAGT+CGG | 0.656767 | 8.4:+60621121 | None:intergenic |
| CAGTGTGATAAGGAAATCAG+TGG | 0.661322 | 8.4:-60621604 | MS.gene95250:CDS |
| AGAAAGCTGAATCCTTCTGG+AGG | 0.662853 | 8.4:+60620224 | None:intergenic |
| GGAGATATATTTCAGCCAAG+TGG | 0.666765 | 8.4:-60623867 | MS.gene95250:CDS |
| AAATGCTTAGAGGATCAACT+AGG | 0.671879 | 8.4:-60626092 | MS.gene95250:CDS |
| CATGGCTAAGACAACTATGG+TGG | 0.672953 | 8.4:-60626730 | MS.gene95250:CDS |
| CAGTCGTTGTCTGAACTGAG+TGG | 0.675822 | 8.4:-60620158 | MS.gene95250:CDS |
| GCCAAGTTAGAACCTCCAGA+AGG | 0.687058 | 8.4:-60620236 | MS.gene95250:CDS |
| CAGGAGCGCGAATCTGAGAG+GGG | 0.689108 | 8.4:-60625743 | MS.gene95250:CDS |
| AGGAGCGAAGAAGTCAACAA+AGG | 0.689164 | 8.4:-60626552 | MS.gene95250:CDS |
| AGGTGTAGGTTACTGCTGCG+AGG | 0.692776 | 8.4:-60621681 | MS.gene95250:intron |
| TCGTTGTCTGAACTGAGTGG+AGG | 0.693325 | 8.4:-60620155 | MS.gene95250:CDS |
| AACCTCTAGGGCAGTCACGG+TGG | 0.694961 | 8.4:+60625574 | None:intergenic |
| AAGCATAGACAATATCTCTG+GGG | 0.695585 | 8.4:+60627052 | None:intergenic |
| ACAGAAAGACTGTTCTGTGA+GGG | 0.698306 | 8.4:-60621210 | MS.gene95250:CDS |
| GAATGAGCTTCATATCCAAG+CGG | 0.703712 | 8.4:+60628539 | None:intergenic |
| TCGGTCGAATCCAGGAACAA+CGG | 0.713375 | 8.4:+60628760 | None:intergenic |
| GGAGCTCTCAAAGAACCTGG+AGG | 0.733098 | 8.4:-60626280 | MS.gene95250:CDS |
| AACAGAAAGACTGTTCTGTG+AGG | 0.738563 | 8.4:-60621211 | MS.gene95250:CDS |
| CTATGCTTGAGGAAGCTGCG+GGG | 0.741808 | 8.4:-60627037 | MS.gene95250:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 60618090 | 60628834 | 60618090 | ID=MS.gene95250 |
| chr8.4 | mRNA | 60618090 | 60628834 | 60618090 | ID=MS.gene95250.t1;Parent=MS.gene95250 |
| chr8.4 | exon | 60628517 | 60628834 | 60628517 | ID=MS.gene95250.t1.exon1;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60628517 | 60628834 | 60628517 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60626548 | 60627228 | 60626548 | ID=MS.gene95250.t1.exon2;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60626548 | 60627228 | 60626548 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60626260 | 60626403 | 60626260 | ID=MS.gene95250.t1.exon3;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60626260 | 60626403 | 60626260 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60625860 | 60626150 | 60625860 | ID=MS.gene95250.t1.exon4;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60625860 | 60626150 | 60625860 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60625577 | 60625762 | 60625577 | ID=MS.gene95250.t1.exon5;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60625577 | 60625762 | 60625577 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60625334 | 60625498 | 60625334 | ID=MS.gene95250.t1.exon6;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60625334 | 60625498 | 60625334 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60625175 | 60625234 | 60625175 | ID=MS.gene95250.t1.exon7;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60625175 | 60625234 | 60625175 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60624860 | 60624922 | 60624860 | ID=MS.gene95250.t1.exon8;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60624860 | 60624922 | 60624860 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60623843 | 60623934 | 60623843 | ID=MS.gene95250.t1.exon9;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60623843 | 60623934 | 60623843 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60623493 | 60623589 | 60623493 | ID=MS.gene95250.t1.exon10;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60623493 | 60623589 | 60623493 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60623002 | 60623118 | 60623002 | ID=MS.gene95250.t1.exon11;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60623002 | 60623118 | 60623002 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60622666 | 60622884 | 60622666 | ID=MS.gene95250.t1.exon12;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60622666 | 60622884 | 60622666 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60621771 | 60621911 | 60621771 | ID=MS.gene95250.t1.exon13;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60621771 | 60621911 | 60621771 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60621528 | 60621695 | 60621528 | ID=MS.gene95250.t1.exon14;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60621528 | 60621695 | 60621528 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60621068 | 60621252 | 60621068 | ID=MS.gene95250.t1.exon15;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60621068 | 60621252 | 60621068 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60620858 | 60620951 | 60620858 | ID=MS.gene95250.t1.exon16;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60620858 | 60620951 | 60620858 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60620696 | 60620784 | 60620696 | ID=MS.gene95250.t1.exon17;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60620696 | 60620784 | 60620696 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60620076 | 60620301 | 60620076 | ID=MS.gene95250.t1.exon18;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60620076 | 60620301 | 60620076 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60618510 | 60618584 | 60618510 | ID=MS.gene95250.t1.exon19;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60618510 | 60618584 | 60618510 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| chr8.4 | exon | 60618090 | 60618206 | 60618090 | ID=MS.gene95250.t1.exon20;Parent=MS.gene95250.t1 |
| chr8.4 | CDS | 60618090 | 60618206 | 60618090 | ID=cds.MS.gene95250.t1;Parent=MS.gene95250.t1 |
| Gene Sequence |
| Protein sequence |
