AlfalfaGEDB | MS.gene021450

M. sativa cultivar XinJiangDaYe / MS.gene021450

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021450.t1	XP_013465692.1	89.1	193	20	1	1	193	1	192	6.90E-91	343.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021450.t1	A0A072VE41	89.1	193	20	1	1	193	1	192	4.9e-91	343.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene021450	MS.gene06909	PPI
MS.gene021450	MS.gene31640	PPI
MS.gene059162	MS.gene021450	PPI
MS.gene057114	MS.gene021450	PPI
MS.gene013579	MS.gene021450	PPI
MS.gene021450	MS.gene059161	PPI
MS.gene059160	MS.gene021450	PPI
MS.gene057116	MS.gene021450	PPI
MS.gene021450	MS.gene54540	PPI
MS.gene06696	MS.gene021450	PPI
MS.gene06909	MS.gene021450	PPI
MS.gene021450	MS.gene06907	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021450.t1	MTR_1g007905	89.119	193	20	1	1	193	1	192	3.06e-123	348
MS.gene021450.t1	MTR_3g091680	54.430	158	57	5	37	193	1	144	1.38e-44	147

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene021450.t1	AT5G53030	38.043	184	73	9	45	193	40	217	1.97e-20	86.3
MS.gene021450.t1	AT5G53030	37.705	183	73	9	45	192	40	216	2.16e-19	84.0

Find 58 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGGCAGTGTGAATTT+TGG	0.173203	1.2:-11980229	MS.gene021450:CDS
TCCTCCTAGGTTGTTGTCTT+TGG	0.204117	1.2:-11980359	MS.gene021450:CDS
ATCATGATGATGATGAATTA+AGG	0.279657	1.2:-11980591	MS.gene021450:CDS
CATTCATGTCTCACTCTTCA+TGG	0.286397	1.2:-11980014	MS.gene021450:CDS
TTGTTACCTTCTCTTTCTTT+TGG	0.308850	1.2:+11980403	None:intergenic
GCCATCACCAACAACGGTTT+TGG	0.333830	1.2:-11980308	MS.gene021450:CDS
TAACAACGTTGTTAGAGCTT+TGG	0.381783	1.2:-11980386	MS.gene021450:CDS
CACATGGTACTTCACATTTG+TGG	0.395630	1.2:-11980075	MS.gene021450:CDS
TTGGGTCTAGTAGGTGGGGT+GGG	0.401963	1.2:-11980210	MS.gene021450:CDS
ACATGGTACTTCACATTTGT+GGG	0.406088	1.2:-11980074	MS.gene021450:CDS
ATAGCCGTCGTTCGCGGTGG+TGG	0.414893	1.2:+11980490	None:intergenic
GATTGTAGTTTCAAATGCTT+AGG	0.415476	1.2:-11979992	MS.gene021450:CDS
TTTAAGTGGGAGGAAGCTCC+AGG	0.425438	1.2:-11980457	MS.gene021450:CDS
TGACATGACCTAGGCTTCCC+TGG	0.444525	1.2:+11980439	None:intergenic
TAAGGTATATAAAATAAGAT+AGG	0.444783	1.2:+11980039	None:intergenic
TTTCAAGTTGGAGTGTTGAT+GGG	0.452572	1.2:-11980144	MS.gene021450:CDS
GTCGTTCGCGGTGGTGGAGT+TGG	0.473237	1.2:+11980496	None:intergenic
GGAGCTTCCTCCCACTTAAA+CGG	0.473694	1.2:+11980460	None:intergenic
TAGGTTGTTGTCTTTGGAGA+GGG	0.474181	1.2:-11980353	MS.gene021450:CDS
GAGTGAGACATGAATGGATA+AGG	0.475677	1.2:+11980021	None:intergenic
GGACCTTCCAAAACCGTTGT+TGG	0.478520	1.2:+11980301	None:intergenic
TCATGATGATGATGAATTAA+GGG	0.486963	1.2:-11980590	MS.gene021450:CDS
TCCAAAACCGTTGTTGGTGA+TGG	0.490839	1.2:+11980307	None:intergenic
ATTGTAGTTTCAAATGCTTA+GGG	0.495991	1.2:-11979991	MS.gene021450:CDS
GAGCTTTGGAACTTCCTCCT+AGG	0.498331	1.2:-11980372	MS.gene021450:CDS
TTTGGGTCTAGTAGGTGGGG+TGG	0.500229	1.2:-11980211	MS.gene021450:CDS
CACGGCCGCGTTAACGTTGG+CGG	0.510236	1.2:+11980535	None:intergenic
CATGAAGAGTGAGACATGAA+TGG	0.515826	1.2:+11980015	None:intergenic
CTAGGTTGTTGTCTTTGGAG+AGG	0.533267	1.2:-11980354	MS.gene021450:CDS
AGGAAGCTCCAGGGAAGCCT+AGG	0.538070	1.2:-11980447	MS.gene021450:CDS
TTCAAGTTGGAGTGTTGATG+GGG	0.539072	1.2:-11980143	MS.gene021450:CDS
CTATATCGATACCGTTTAAG+TGG	0.539738	1.2:-11980471	MS.gene021450:CDS
AACTCCACCACCGCGAACGA+CGG	0.539846	1.2:-11980494	MS.gene021450:CDS
GCTTCCCCTTCTCTTAACTC+TGG	0.540867	1.2:+11980102	None:intergenic
GACACAGCTCTTCCAACGTA+AGG	0.545767	1.2:+11980280	None:intergenic
ATCGATATAGCCGTCGTTCG+CGG	0.559277	1.2:+11980484	None:intergenic
GATCACCAGAGTTAAGAGAA+GGG	0.560447	1.2:-11980107	MS.gene021450:CDS
ATCACCAGAGTTAAGAGAAG+GGG	0.578795	1.2:-11980106	MS.gene021450:CDS
AGATCACCAGAGTTAAGAGA+AGG	0.583244	1.2:-11980108	MS.gene021450:CDS
GGTCGTGTGTGACATGACCT+AGG	0.597767	1.2:+11980430	None:intergenic
ATCGATACCGTTTAAGTGGG+AGG	0.600972	1.2:-11980467	MS.gene021450:CDS
CTCTTCCGCCAACGTTAACG+CGG	0.609061	1.2:-11980540	MS.gene021450:CDS
CATGATGATGATGAATTAAG+GGG	0.613597	1.2:-11980589	MS.gene021450:CDS
CCAGAGCCAAAAGAAAGAGA+AGG	0.615358	1.2:-11980409	MS.gene021450:CDS
AAACACGGCCGCGTTAACGT+TGG	0.618006	1.2:+11980532	None:intergenic
GTATAGGGATAGTAATAACA+AGG	0.626999	1.2:-11980245	MS.gene021450:CDS
TTACGCGCCATCACCAACAA+CGG	0.631163	1.2:-11980314	MS.gene021450:CDS
TCCAAAGACAACAACCTAGG+AGG	0.639929	1.2:+11980358	None:intergenic
TATATCGATACCGTTTAAGT+GGG	0.641926	1.2:-11980470	MS.gene021450:CDS
TCAAGTTGGAGTGTTGATGG+GGG	0.642096	1.2:-11980142	MS.gene021450:CDS
AAAAGTCAAAACTACCCTCA+CGG	0.642581	1.2:+11980164	None:intergenic
AATAATAGAATTGACCGTGA+GGG	0.656653	1.2:-11980178	MS.gene021450:CDS
CTCTCCAAAGACAACAACCT+AGG	0.661580	1.2:+11980355	None:intergenic
TTAAGTGGGAGGAAGCTCCA+GGG	0.684371	1.2:-11980456	MS.gene021450:CDS
AAATAATAGAATTGACCGTG+AGG	0.684752	1.2:-11980179	MS.gene021450:CDS
GATATAGCCGTCGTTCGCGG+TGG	0.697862	1.2:+11980487	None:intergenic
TATAGGGATAGTAATAACAA+GGG	0.698970	1.2:-11980244	MS.gene021450:CDS
AGAAGGGGAAGCTTCTCACA+TGG	0.717228	1.2:-11980091	MS.gene021450:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAGGTATATAAAATAAGAT+AGG	+	chr1.2:11980562-11980581	None:intergenic	15.0%
!	ATTGTAGTTTCAAATGCTTA+GGG	-	chr1.2:11980607-11980626	MS.gene021450:CDS	25.0%
!	TATAGGGATAGTAATAACAA+GGG	-	chr1.2:11980354-11980373	MS.gene021450:CDS	25.0%
!!	ATCATGATGATGATGAATTA+AGG	-	chr1.2:11980007-11980026	MS.gene021450:CDS	25.0%
!!	TCATGATGATGATGAATTAA+GGG	-	chr1.2:11980008-11980027	MS.gene021450:CDS	25.0%
	AAATAATAGAATTGACCGTG+AGG	-	chr1.2:11980419-11980438	MS.gene021450:CDS	30.0%
	AATAATAGAATTGACCGTGA+GGG	-	chr1.2:11980420-11980439	MS.gene021450:CDS	30.0%
	GATTGTAGTTTCAAATGCTT+AGG	-	chr1.2:11980606-11980625	MS.gene021450:CDS	30.0%
	GTATAGGGATAGTAATAACA+AGG	-	chr1.2:11980353-11980372	MS.gene021450:CDS	30.0%
	TATATCGATACCGTTTAAGT+GGG	-	chr1.2:11980128-11980147	MS.gene021450:CDS	30.0%
!	CATGATGATGATGAATTAAG+GGG	-	chr1.2:11980009-11980028	MS.gene021450:CDS	30.0%
!	TTGTTACCTTCTCTTTCTTT+TGG	+	chr1.2:11980198-11980217	None:intergenic	30.0%
!!!	GTAGTTTTGACTTTTCAAGT+TGG	-	chr1.2:11980442-11980461	MS.gene021450:CDS	30.0%
	AAAAGTCAAAACTACCCTCA+CGG	+	chr1.2:11980437-11980456	None:intergenic	35.0%
	ACATGGTACTTCACATTTGT+GGG	-	chr1.2:11980524-11980543	MS.gene021450:CDS	35.0%
	CTATATCGATACCGTTTAAG+TGG	-	chr1.2:11980127-11980146	MS.gene021450:CDS	35.0%
	TAACAACGTTGTTAGAGCTT+TGG	-	chr1.2:11980212-11980231	MS.gene021450:CDS	35.0%
!!	TTTCAAGTTGGAGTGTTGAT+GGG	-	chr1.2:11980454-11980473	MS.gene021450:CDS	35.0%
!!!	TTTTCAAGTTGGAGTGTTGA+TGG	-	chr1.2:11980453-11980472	MS.gene021450:CDS	35.0%
	AGATCACCAGAGTTAAGAGA+AGG	-	chr1.2:11980490-11980509	MS.gene021450:CDS	40.0%
	ATCACCAGAGTTAAGAGAAG+GGG	-	chr1.2:11980492-11980511	MS.gene021450:CDS	40.0%
	CACATGGTACTTCACATTTG+TGG	-	chr1.2:11980523-11980542	MS.gene021450:CDS	40.0%
	CATGAAGAGTGAGACATGAA+TGG	+	chr1.2:11980586-11980605	None:intergenic	40.0%
	CATTCATGTCTCACTCTTCA+TGG	-	chr1.2:11980584-11980603	MS.gene021450:CDS	40.0%
	GAGTGAGACATGAATGGATA+AGG	+	chr1.2:11980580-11980599	None:intergenic	40.0%
	GATCACCAGAGTTAAGAGAA+GGG	-	chr1.2:11980491-11980510	MS.gene021450:CDS	40.0%
!	AACAAGGGCAGTGTGAATTT+TGG	-	chr1.2:11980369-11980388	MS.gene021450:CDS	40.0%
!	ACAAGGGCAGTGTGAATTTT+GGG	-	chr1.2:11980370-11980389	MS.gene021450:CDS	40.0%
!	GTCTTTTACGACGTCGTATA+GGG	-	chr1.2:11980338-11980357	MS.gene021450:CDS	40.0%
!	TAGGTTGTTGTCTTTGGAGA+GGG	-	chr1.2:11980245-11980264	MS.gene021450:CDS	40.0%
!	TGTCTTTTACGACGTCGTAT+AGG	-	chr1.2:11980337-11980356	MS.gene021450:CDS	40.0%
!!	AATTTTGGGTCTAGTAGGTG+GGG	-	chr1.2:11980384-11980403	MS.gene021450:CDS	40.0%
!!	GAATTTTGGGTCTAGTAGGT+GGG	-	chr1.2:11980383-11980402	MS.gene021450:CDS	40.0%
!!	GTGTGAATTTTGGGTCTAGT+AGG	-	chr1.2:11980379-11980398	MS.gene021450:CDS	40.0%
!!	TGAATTTTGGGTCTAGTAGG+TGG	-	chr1.2:11980382-11980401	MS.gene021450:CDS	40.0%
!!	TTCAAGTTGGAGTGTTGATG+GGG	-	chr1.2:11980455-11980474	MS.gene021450:CDS	40.0%
	ATCGATACCGTTTAAGTGGG+AGG	-	chr1.2:11980131-11980150	MS.gene021450:CDS	45.0%
	CCAGAGCCAAAAGAAAGAGA+AGG	-	chr1.2:11980189-11980208	MS.gene021450:CDS	45.0%
	CTCTCCAAAGACAACAACCT+AGG	+	chr1.2:11980246-11980265	None:intergenic	45.0%
	TCCAAAGACAACAACCTAGG+AGG	+	chr1.2:11980243-11980262	None:intergenic	45.0%
	TCCTCCTAGGTTGTTGTCTT+TGG	-	chr1.2:11980239-11980258	MS.gene021450:CDS	45.0%
!	CTAGGTTGTTGTCTTTGGAG+AGG	-	chr1.2:11980244-11980263	MS.gene021450:CDS	45.0%
!	GGCGGAAGAGAAAGTTTTTG+TGG	+	chr1.2:11980048-11980067	None:intergenic	45.0%
!	TCCAAAACCGTTGTTGGTGA+TGG	+	chr1.2:11980294-11980313	None:intergenic	45.0%
!!	TCAAGTTGGAGTGTTGATGG+GGG	-	chr1.2:11980456-11980475	MS.gene021450:CDS	45.0%
!!!	CCTTCTCTTTCTTTTGGCTC+TGG	+	chr1.2:11980192-11980211	None:intergenic	45.0%
!!!	GTTTTGGAAGGTCCTTACGT+TGG	-	chr1.2:11980306-11980325	MS.gene021450:CDS	45.0%
!!!	TCACCAACAACGGTTTTGGA+AGG	-	chr1.2:11980294-11980313	MS.gene021450:CDS	45.0%
	AGAAGGGGAAGCTTCTCACA+TGG	-	chr1.2:11980507-11980526	MS.gene021450:CDS	50.0%
	ATCGATATAGCCGTCGTTCG+CGG	+	chr1.2:11980117-11980136	None:intergenic	50.0%
	GACACAGCTCTTCCAACGTA+AGG	+	chr1.2:11980321-11980340	None:intergenic	50.0%
	GCTTCCCCTTCTCTTAACTC+TGG	+	chr1.2:11980499-11980518	None:intergenic	50.0%
	GGACCTTCCAAAACCGTTGT+TGG	+	chr1.2:11980300-11980319	None:intergenic	50.0%
	GGAGCTTCCTCCCACTTAAA+CGG	+	chr1.2:11980141-11980160	None:intergenic	50.0%
	TTAAGTGGGAGGAAGCTCCA+GGG	-	chr1.2:11980142-11980161	MS.gene021450:CDS	50.0%
	TTACGCGCCATCACCAACAA+CGG	-	chr1.2:11980284-11980303	MS.gene021450:CDS	50.0%
	TTTAAGTGGGAGGAAGCTCC+AGG	-	chr1.2:11980141-11980160	MS.gene021450:CDS	50.0%
!	GCCATCACCAACAACGGTTT+TGG	-	chr1.2:11980290-11980309	MS.gene021450:CDS	50.0%
!!	GAGCTTTGGAACTTCCTCCT+AGG	-	chr1.2:11980226-11980245	MS.gene021450:CDS	50.0%
!!	GAGTGTTGATGGGGGTGATA+AGG	-	chr1.2:11980464-11980483	MS.gene021450:CDS	50.0%
	AAACACGGCCGCGTTAACGT+TGG	+	chr1.2:11980069-11980088	None:intergenic	55.0%
	CTCTTCCGCCAACGTTAACG+CGG	-	chr1.2:11980058-11980077	MS.gene021450:CDS	55.0%
	GGTCGTGTGTGACATGACCT+AGG	+	chr1.2:11980171-11980190	None:intergenic	55.0%
	GGTGCCTCCGACGAAAAACA+CGG	+	chr1.2:11980084-11980103	None:intergenic	55.0%
	TGACATGACCTAGGCTTCCC+TGG	+	chr1.2:11980162-11980181	None:intergenic	55.0%
	TTGGGTCTAGTAGGTGGGGT+GGG	-	chr1.2:11980388-11980407	MS.gene021450:CDS	55.0%
	TTTGGGTCTAGTAGGTGGGG+TGG	-	chr1.2:11980387-11980406	MS.gene021450:CDS	55.0%
!	AACGCGGCCGTGTTTTTCGT+CGG	-	chr1.2:11980074-11980093	MS.gene021450:CDS	55.0%
	AACTCCACCACCGCGAACGA+CGG	-	chr1.2:11980104-11980123	MS.gene021450:CDS	60.0%
	AGGAAGCTCCAGGGAAGCCT+AGG	-	chr1.2:11980151-11980170	MS.gene021450:CDS	60.0%
	GATATAGCCGTCGTTCGCGG+TGG	+	chr1.2:11980114-11980133	None:intergenic	60.0%
	ATAGCCGTCGTTCGCGGTGG+TGG	+	chr1.2:11980111-11980130	None:intergenic	65.0%
	CACGGCCGCGTTAACGTTGG+CGG	+	chr1.2:11980066-11980085	None:intergenic	65.0%
!	GCGGCCGTGTTTTTCGTCGG+AGG	-	chr1.2:11980077-11980096	MS.gene021450:CDS	65.0%
!!	GTCGTTCGCGGTGGTGGAGT+TGG	+	chr1.2:11980105-11980124	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	11979979	11980641	11979979	ID=MS.gene021450
chr1.2	mRNA	11979979	11980641	11979979	ID=MS.gene021450.t1;Parent=MS.gene021450
chr1.2	exon	11979979	11980641	11979979	ID=MS.gene021450.t1.exon1;Parent=MS.gene021450.t1
chr1.2	CDS	11979979	11980641	11979979	ID=cds.MS.gene021450.t1;Parent=MS.gene021450.t1

Gene Sequence

>MS.gene021450

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGGGCAACTACAACACACCACAAAAACTTTCTCTTCCGCCAACGTTAACGCGGCCGTGTTTTTCGTCGGAGGCACCAACTCCACCACCGCGAACGACGGCTATATCGATACCGTTTAAGTGGGAGGAAGCTCCAGGGAAGCCTAGGTCATGTCACACACGACCAGAGCCAAAAGAAAGAGAAGGTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCGCCATCACCAACAACGGTTTTGGAAGGTCCTTACGTTGGAAGAGCTGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACCAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGGGTATGTCTCTTTACCTATCTTATTTTATATACCTTATCCATTCATGTCTCACTCTTCATGGATTGTAGTTTCAAATGCTTAGGGATAGTTCTTTAA

Protein sequence

>MS.gene021450.t1

MTSENNKKLDHDDDELRGNYNTPQKLSLPPTLTRPCFSSEAPTPPPRTTAISIPFKWEEAPGKPRSCHTRPEPKEREGNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLWVCLFTYLILYTLSIHVSLFMDCSFKCLGIVL