Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057114.t1 | XP_003602268.1 | 96.5 | 141 | 4 | 1 | 1 | 141 | 1 | 140 | 9.80E-70 | 272.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057114.t1 | G7J7F8 | 96.5 | 141 | 4 | 1 | 1 | 141 | 1 | 140 | 7.1e-70 | 272.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057114 | MS.gene057116 | 0.982605 | 1.59E-155 | -1.69E-46 |
| MS.gene057114 | MS.gene06907 | 0.85322 | 2.76E-61 | -1.69E-46 |
| MS.gene057114 | MS.gene06909 | 0.842311 | 2.79E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene013579 | MS.gene057114 | PPI |
| MS.gene057114 | MS.gene057116 | PPI |
| MS.gene057114 | MS.gene06696 | PPI |
| MS.gene059160 | MS.gene057114 | PPI |
| MS.gene057116 | MS.gene057114 | PPI |
| MS.gene057114 | MS.gene059161 | PPI |
| MS.gene057114 | MS.gene54540 | PPI |
| MS.gene057114 | MS.gene021450 | PPI |
| MS.gene057114 | MS.gene06907 | PPI |
| MS.gene057114 | MS.gene059162 | PPI |
| MS.gene06696 | MS.gene057114 | PPI |
| MS.gene057114 | MS.gene013579 | PPI |
| MS.gene057114 | MS.gene31640 | PPI |
| MS.gene057114 | MS.gene059160 | PPI |
| MS.gene059162 | MS.gene057114 | PPI |
| MS.gene057114 | MS.gene06909 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057114.t1 | MTR_3g091680 | 96.454 | 141 | 4 | 1 | 1 | 141 | 1 | 140 | 1.37e-96 | 275 |
| MS.gene057114.t1 | MTR_1g007905 | 55.844 | 154 | 54 | 5 | 1 | 141 | 36 | 188 | 1.26e-44 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057114.t1 | AT5G53030 | 36.313 | 179 | 72 | 7 | 5 | 142 | 37 | 214 | 2.20e-17 | 76.3 |
| MS.gene057114.t1 | AT5G53030 | 35.754 | 179 | 73 | 7 | 5 | 142 | 37 | 214 | 6.32e-17 | 74.7 |
| MS.gene057114.t1 | AT4G27810 | 36.496 | 137 | 64 | 4 | 5 | 131 | 33 | 156 | 1.37e-11 | 59.7 |
Find 52 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACTGGAATTCCAATTT+TGG | 0.127410 | 3.1:+73602643 | MS.gene057114:CDS |
| CTAGCAGGTGGAGTGGTTTC+AGG | 0.269736 | 3.1:+73602668 | MS.gene057114:CDS |
| CCACCTGCTAGACCCAAAAT+TGG | 0.283838 | 3.1:-73602656 | None:intergenic |
| TCACCTACAACTGTGTTAGA+TGG | 0.315146 | 3.1:+73602568 | MS.gene057114:CDS |
| ATAGAACTCCAAGGGAAAAC+TGG | 0.328757 | 3.1:+73602629 | MS.gene057114:CDS |
| TGGTTTCAGGAAGATTAATA+AGG | 0.332948 | 3.1:+73602681 | MS.gene057114:CDS |
| CTTGAAAGGAATTGAAATCA+TGG | 0.366715 | 3.1:-73602419 | None:intergenic |
| AAAGGGAAAATCACATTTGT+GGG | 0.419238 | 3.1:+73602786 | MS.gene057114:CDS |
| TGGTAGTGCCTAGGCTTCCC+TGG | 0.422749 | 3.1:-73602454 | None:intergenic |
| CAACCTTGGAACTGCCTCCT+AGG | 0.434449 | 3.1:+73602509 | MS.gene057114:CDS |
| CAAAGGGAAAATCACATTTG+TGG | 0.438574 | 3.1:+73602785 | MS.gene057114:CDS |
| TAGATGGGCCTTACGTGGGC+CGG | 0.448015 | 3.1:+73602584 | MS.gene057114:CDS |
| ATTAATAAGGAAGGAGATGA+GGG | 0.450852 | 3.1:+73602694 | MS.gene057114:CDS |
| TGAAGGAACATCAACCTTGT+TGG | 0.458738 | 3.1:-73602548 | None:intergenic |
| TTCAAGTGGGAGGAAGCACC+AGG | 0.461383 | 3.1:+73602436 | MS.gene057114:CDS |
| CTGTATTGTCGAGCTCTGAT+TGG | 0.465440 | 3.1:-73602480 | None:intergenic |
| AGATGGGCCTTACGTGGGCC+GGG | 0.475085 | 3.1:+73602585 | MS.gene057114:CDS |
| GGAATTGAAATCATGGTTTG+AGG | 0.484375 | 3.1:-73602412 | None:intergenic |
| TTCAGGAAGATTAATAAGGA+AGG | 0.487183 | 3.1:+73602685 | MS.gene057114:CDS |
| GGAGGAAGTCCCATTTCAAA+GGG | 0.501251 | 3.1:+73602769 | MS.gene057114:CDS |
| AGGAAGCACCAGGGAAGCCT+AGG | 0.504051 | 3.1:+73602446 | MS.gene057114:CDS |
| AAGATGTCACGGGCTGCTAA+AGG | 0.520498 | 3.1:+73602748 | MS.gene057114:CDS |
| GGCCCATCTAACACAGTTGT+AGG | 0.520900 | 3.1:-73602571 | None:intergenic |
| GACATGGCCCGGCCCACGTA+AGG | 0.522178 | 3.1:-73602592 | None:intergenic |
| GATTGGGTGTGGTAGTGCCT+AGG | 0.526775 | 3.1:-73602463 | None:intergenic |
| GATTAATAAGGAAGGAGATG+AGG | 0.528348 | 3.1:+73602693 | MS.gene057114:CDS |
| AGGAGGAAGTCCCATTTCAA+AGG | 0.530152 | 3.1:+73602768 | MS.gene057114:CDS |
| TGTATTGTCGAGCTCTGATT+GGG | 0.531093 | 3.1:-73602479 | None:intergenic |
| CACCTACAACTGTGTTAGAT+GGG | 0.532506 | 3.1:+73602569 | MS.gene057114:CDS |
| AAGAAGTGAAGGACATGGCC+CGG | 0.535442 | 3.1:-73602603 | None:intergenic |
| GGTGCTTCCTCCCACTTGAA+AGG | 0.541875 | 3.1:-73602433 | None:intergenic |
| TGATTTCAATTCCTTTCAAG+TGG | 0.550003 | 3.1:+73602422 | MS.gene057114:CDS |
| TTCAATTCCTTTCAAGTGGG+AGG | 0.551211 | 3.1:+73602426 | MS.gene057114:CDS |
| TTTGGGTCTAGCAGGTGGAG+TGG | 0.556268 | 3.1:+73602661 | MS.gene057114:CDS |
| TGTGTTAGATGGGCCTTACG+TGG | 0.559160 | 3.1:+73602579 | MS.gene057114:CDS |
| CAATACAGTTAGAACAACCT+TGG | 0.565902 | 3.1:+73602495 | MS.gene057114:CDS |
| TCAACAACCAATAATGTCAT+CGG | 0.578794 | 3.1:+73602372 | None:intergenic |
| AGAATAAGGTGAAGATGTCA+CGG | 0.584256 | 3.1:+73602737 | MS.gene057114:CDS |
| GATTTCAATTCCTTTCAAGT+GGG | 0.605733 | 3.1:+73602423 | MS.gene057114:CDS |
| CTTCTTCCTATAGAACTCCA+AGG | 0.606398 | 3.1:+73602620 | MS.gene057114:CDS |
| TGTCGAGCTCTGATTGGGTG+TGG | 0.614054 | 3.1:-73602474 | None:intergenic |
| CATGGTTTGAGGTGGTGACA+TGG | 0.621041 | 3.1:-73602401 | None:intergenic |
| ATGTCACGGGCTGCTAAAGG+AGG | 0.635685 | 3.1:+73602751 | MS.gene057114:CDS |
| CAACCTAGGAGGCAGTTCCA+AGG | 0.636697 | 3.1:-73602512 | None:intergenic |
| GAATAAGGTGAAGATGTCAC+GGG | 0.637192 | 3.1:+73602738 | MS.gene057114:CDS |
| TCTAACACAGTTGTAGGTGA+AGG | 0.645082 | 3.1:-73602565 | None:intergenic |
| TTCTTCCTATAGAACTCCAA+GGG | 0.650470 | 3.1:+73602621 | MS.gene057114:CDS |
| GTGTTAGATGGGCCTTACGT+GGG | 0.653028 | 3.1:+73602580 | MS.gene057114:CDS |
| AGTTCTATAGGAAGAAGTGA+AGG | 0.661579 | 3.1:-73602614 | None:intergenic |
| ATTGAAATCATGGTTTGAGG+TGG | 0.684990 | 3.1:-73602409 | None:intergenic |
| ATAGGAAGAAGTGAAGGACA+TGG | 0.705802 | 3.1:-73602608 | None:intergenic |
| TCAAGTGGGAGGAAGCACCA+GGG | 0.732644 | 3.1:+73602437 | MS.gene057114:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAAGTATTTCTATATATAGT+TGG | + | chr3.1:73603201-73603220 | MS.gene057114:intron | 15.0% |
| !! | AAAAGAGAGAATGATAATTT+AGG | - | chr3.1:73603135-73603154 | None:intergenic | 20.0% |
| !! | AAAATGAAAGACAAATTTGA+TGG | + | chr3.1:73603165-73603184 | MS.gene057114:intron | 20.0% |
| !! | ATGTATGTAGTCTTTATTAT+AGG | - | chr3.1:73603013-73603032 | None:intergenic | 20.0% |
| !!! | AAATTAGTTTAAGGACTATT+TGG | - | chr3.1:73603095-73603114 | None:intergenic | 20.0% |
| !!! | ATTTTGCTTATATTAGTGAT+CGG | + | chr3.1:73603061-73603080 | MS.gene057114:intron | 20.0% |
| !!! | GATAGATTCAAATTAGTTTA+AGG | - | chr3.1:73603104-73603123 | None:intergenic | 20.0% |
| ! | CTAATTTGAATCTATCAAGA+TGG | + | chr3.1:73603108-73603127 | MS.gene057114:intron | 25.0% |
| !! | AAAACTGGAATTCCAATTTT+GGG | + | chr3.1:73602644-73602663 | MS.gene057114:CDS | 25.0% |
| !! | CTTTTGCTTATATTAGTGAT+TGG | - | chr3.1:73602952-73602971 | None:intergenic | 25.0% |
| !!! | TATATTGATGCAAAAGCATT+TGG | - | chr3.1:73602865-73602884 | None:intergenic | 25.0% |
| AAAGGGAAAATCACATTTGT+GGG | + | chr3.1:73602786-73602805 | MS.gene057114:CDS | 30.0% | |
| ATTAATAAGGAAGGAGATGA+GGG | + | chr3.1:73602694-73602713 | MS.gene057114:CDS | 30.0% | |
| CTTGAAAGGAATTGAAATCA+TGG | - | chr3.1:73602422-73602441 | None:intergenic | 30.0% | |
| TGATTTCAATTCCTTTCAAG+TGG | + | chr3.1:73602422-73602441 | MS.gene057114:CDS | 30.0% | |
| TGGTTTCAGGAAGATTAATA+AGG | + | chr3.1:73602681-73602700 | MS.gene057114:CDS | 30.0% | |
| TTCAGGAAGATTAATAAGGA+AGG | + | chr3.1:73602685-73602704 | MS.gene057114:CDS | 30.0% | |
| ! | GAAAACTGGAATTCCAATTT+TGG | + | chr3.1:73602643-73602662 | MS.gene057114:CDS | 30.0% |
| ! | GATTTCAATTCCTTTCAAGT+GGG | + | chr3.1:73602423-73602442 | MS.gene057114:CDS | 30.0% |
| !! | ATGTGATTTTCCCTTTGAAA+TGG | - | chr3.1:73602782-73602801 | None:intergenic | 30.0% |
| !!! | TGTGATTTTCCCTTTGAAAT+GGG | - | chr3.1:73602781-73602800 | None:intergenic | 30.0% |
| AGAATAAGGTGAAGATGTCA+CGG | + | chr3.1:73602737-73602756 | MS.gene057114:CDS | 35.0% | |
| AGTTCTATAGGAAGAAGTGA+AGG | - | chr3.1:73602617-73602636 | None:intergenic | 35.0% | |
| ATTGAAATCATGGTTTGAGG+TGG | - | chr3.1:73602412-73602431 | None:intergenic | 35.0% | |
| CAAAGGGAAAATCACATTTG+TGG | + | chr3.1:73602785-73602804 | MS.gene057114:CDS | 35.0% | |
| CAATACAGTTAGAACAACCT+TGG | + | chr3.1:73602495-73602514 | MS.gene057114:CDS | 35.0% | |
| GATTAATAAGGAAGGAGATG+AGG | + | chr3.1:73602693-73602712 | MS.gene057114:CDS | 35.0% | |
| GGAATTGAAATCATGGTTTG+AGG | - | chr3.1:73602415-73602434 | None:intergenic | 35.0% | |
| TTCTTCCTATAGAACTCCAA+GGG | + | chr3.1:73602621-73602640 | MS.gene057114:CDS | 35.0% | |
| TTGCTTATATTAGTGATCGG+AGG | + | chr3.1:73603064-73603083 | MS.gene057114:intron | 35.0% | |
| !!! | TGAGGGTAGTTTTGACTTTT+CGG | + | chr3.1:73602711-73602730 | MS.gene057114:CDS | 35.0% |
| ATAGAACTCCAAGGGAAAAC+TGG | + | chr3.1:73602629-73602648 | MS.gene057114:CDS | 40.0% | |
| ATAGGAAGAAGTGAAGGACA+TGG | - | chr3.1:73602611-73602630 | None:intergenic | 40.0% | |
| CACCTACAACTGTGTTAGAT+GGG | + | chr3.1:73602569-73602588 | MS.gene057114:CDS | 40.0% | |
| CTTCTTCCTATAGAACTCCA+AGG | + | chr3.1:73602620-73602639 | MS.gene057114:CDS | 40.0% | |
| GAATAAGGTGAAGATGTCAC+GGG | + | chr3.1:73602738-73602757 | MS.gene057114:CDS | 40.0% | |
| GCTTCCAAAAATAGCAACCT+AGG | - | chr3.1:73602529-73602548 | None:intergenic | 40.0% | |
| TCACCTACAACTGTGTTAGA+TGG | + | chr3.1:73602568-73602587 | MS.gene057114:CDS | 40.0% | |
| TCCAAAAATAGCAACCTAGG+AGG | - | chr3.1:73602526-73602545 | None:intergenic | 40.0% | |
| TCTAACACAGTTGTAGGTGA+AGG | - | chr3.1:73602568-73602587 | None:intergenic | 40.0% | |
| TGAAGGAACATCAACCTTGT+TGG | - | chr3.1:73602551-73602570 | None:intergenic | 40.0% | |
| ! | GTTTTCCCTTGGAGTTCTAT+AGG | - | chr3.1:73602629-73602648 | None:intergenic | 40.0% |
| ! | TGTATTGTCGAGCTCTGATT+GGG | - | chr3.1:73602482-73602501 | None:intergenic | 40.0% |
| ! | TTCAATTCCTTTCAAGTGGG+AGG | + | chr3.1:73602426-73602445 | MS.gene057114:CDS | 40.0% |
| ! | TTGGAATTCCAGTTTTCCCT+TGG | - | chr3.1:73602640-73602659 | None:intergenic | 40.0% |
| !! | ATTCCAATTTTGGGTCTAGC+AGG | + | chr3.1:73602653-73602672 | MS.gene057114:CDS | 40.0% |
| !!! | GAGGGTAGTTTTGACTTTTC+GGG | + | chr3.1:73602712-73602731 | MS.gene057114:CDS | 40.0% |
| !!! | GCTATTTTTGGAAGCCAACA+AGG | + | chr3.1:73602534-73602553 | MS.gene057114:CDS | 40.0% |
| !!! | TGACTTTTCGGGTCAGAATA+AGG | + | chr3.1:73602723-73602742 | MS.gene057114:CDS | 40.0% |
| AGGAGGAAGTCCCATTTCAA+AGG | + | chr3.1:73602768-73602787 | MS.gene057114:CDS | 45.0% | |
| GGAGGAAGTCCCATTTCAAA+GGG | + | chr3.1:73602769-73602788 | MS.gene057114:CDS | 45.0% | |
| ! | CTGTATTGTCGAGCTCTGAT+TGG | - | chr3.1:73602483-73602502 | None:intergenic | 45.0% |
| !!! | GCCTCCTAGGTTGCTATTTT+TGG | + | chr3.1:73602522-73602541 | MS.gene057114:CDS | 45.0% |
| AAGAAGTGAAGGACATGGCC+CGG | - | chr3.1:73602606-73602625 | None:intergenic | 50.0% | |
| CCACCTGCTAGACCCAAAAT+TGG | - | chr3.1:73602659-73602678 | None:intergenic | 50.0% | |
| GGCCCATCTAACACAGTTGT+AGG | - | chr3.1:73602574-73602593 | None:intergenic | 50.0% | |
| GTGTTAGATGGGCCTTACGT+GGG | + | chr3.1:73602580-73602599 | MS.gene057114:CDS | 50.0% | |
| TGTGTTAGATGGGCCTTACG+TGG | + | chr3.1:73602579-73602598 | MS.gene057114:CDS | 50.0% | |
| ! | AAGATGTCACGGGCTGCTAA+AGG | + | chr3.1:73602748-73602767 | MS.gene057114:CDS | 50.0% |
| ! | CATGGTTTGAGGTGGTGACA+TGG | - | chr3.1:73602404-73602423 | None:intergenic | 50.0% |
| !! | CCAATTTTGGGTCTAGCAGG+TGG | + | chr3.1:73602656-73602675 | MS.gene057114:CDS | 50.0% |
| CAACCTAGGAGGCAGTTCCA+AGG | - | chr3.1:73602515-73602534 | None:intergenic | 55.0% | |
| CAACCTTGGAACTGCCTCCT+AGG | + | chr3.1:73602509-73602528 | MS.gene057114:CDS | 55.0% | |
| CTAGCAGGTGGAGTGGTTTC+AGG | + | chr3.1:73602668-73602687 | MS.gene057114:CDS | 55.0% | |
| GGTGCTTCCTCCCACTTGAA+AGG | - | chr3.1:73602436-73602455 | None:intergenic | 55.0% | |
| TTTGGGTCTAGCAGGTGGAG+TGG | + | chr3.1:73602661-73602680 | MS.gene057114:CDS | 55.0% | |
| ! | ATGTCACGGGCTGCTAAAGG+AGG | + | chr3.1:73602751-73602770 | MS.gene057114:CDS | 55.0% |
| ! | GATTGGGTGTGGTAGTGCCT+AGG | - | chr3.1:73602466-73602485 | None:intergenic | 55.0% |
| ! | TGTCGAGCTCTGATTGGGTG+TGG | - | chr3.1:73602477-73602496 | None:intergenic | 55.0% |
| !! | TCAAGTGGGAGGAAGCACCA+GGG | + | chr3.1:73602437-73602456 | MS.gene057114:CDS | 55.0% |
| !! | TTCAAGTGGGAGGAAGCACC+AGG | + | chr3.1:73602436-73602455 | MS.gene057114:CDS | 55.0% |
| TAGATGGGCCTTACGTGGGC+CGG | + | chr3.1:73602584-73602603 | MS.gene057114:CDS | 60.0% | |
| ! | TGGTAGTGCCTAGGCTTCCC+TGG | - | chr3.1:73602457-73602476 | None:intergenic | 60.0% |
| !! | AGGAAGCACCAGGGAAGCCT+AGG | + | chr3.1:73602446-73602465 | MS.gene057114:CDS | 60.0% |
| AGATGGGCCTTACGTGGGCC+GGG | + | chr3.1:73602585-73602604 | MS.gene057114:CDS | 65.0% | |
| GACATGGCCCGGCCCACGTA+AGG | - | chr3.1:73602595-73602614 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 73602385 | 73603234 | 73602385 | ID=MS.gene057114 |
| chr3.1 | mRNA | 73602385 | 73603234 | 73602385 | ID=MS.gene057114.t1;Parent=MS.gene057114 |
| chr3.1 | exon | 73602385 | 73602807 | 73602385 | ID=MS.gene057114.t1.exon1;Parent=MS.gene057114.t1 |
| chr3.1 | CDS | 73602385 | 73602807 | 73602385 | ID=cds.MS.gene057114.t1;Parent=MS.gene057114.t1 |
| chr3.1 | exon | 73603220 | 73603234 | 73603220 | ID=MS.gene057114.t1.exon2;Parent=MS.gene057114.t1 |
| chr3.1 | CDS | 73603220 | 73603234 | 73603220 | ID=cds.MS.gene057114.t1;Parent=MS.gene057114.t1 |
| Gene Sequence |
| Protein sequence |
