AlfalfaGEDB | MS.gene059160

M. sativa cultivar XinJiangDaYe / MS.gene059160

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059160.t1	XP_013465692.1	91	189	16	1	1	189	1	188	4.50E-91	344

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059160.t1	A0A072VE41	91.0	189	16	1	1	189	1	188	3.3e-91	344.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053166	MS.gene059160	0.813141	2.97E-51	-1.69E-46
MS.gene055701	MS.gene059160	0.824375	8.35E-54	-1.69E-46
MS.gene059160	MS.gene059161	0.915782	3.91E-85	-1.69E-46
MS.gene059160	MS.gene059162	0.989339	1.07E-177	-1.69E-46
MS.gene059160	MS.gene059609	0.8078	4.23E-50	-1.69E-46
MS.gene059160	MS.gene061506	0.812523	4.06E-51	-1.69E-46
MS.gene059160	MS.gene072375	-0.810274	1.25E-50	-1.69E-46
MS.gene059160	MS.gene074184	-0.812885	3.38E-51	-1.69E-46
MS.gene059160	MS.gene074393	-0.80244	5.60E-49	-1.69E-46
MS.gene059160	MS.gene20354	0.840173	1.02E-57	-1.69E-46
MS.gene059160	MS.gene26487	0.803512	3.36E-49	-1.69E-46
MS.gene059160	MS.gene37086	0.814266	1.68E-51	-1.69E-46
MS.gene059160	MS.gene53156	0.812178	4.83E-51	-1.69E-46
MS.gene059160	MS.gene54540	0.928305	3.48E-92	-1.69E-46
MS.gene059160	MS.gene58503	0.802962	4.37E-49	-1.69E-46
MS.gene059160	MS.gene59948	0.801638	8.19E-49	-1.69E-46
MS.gene059160	MS.gene61667	0.801775	7.68E-49	-1.69E-46
MS.gene059160	MS.gene66208	0.814665	1.37E-51	-1.69E-46
MS.gene059160	MS.gene68528	0.800055	1.72E-48	-1.69E-46
MS.gene059160	MS.gene70021	0.807625	4.61E-50	-1.69E-46
MS.gene059160	MS.gene87669	0.806836	6.78E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene013579	MS.gene059160	PPI
MS.gene059160	MS.gene057116	PPI
MS.gene057116	MS.gene059160	PPI
MS.gene059160	MS.gene057114	PPI
MS.gene059160	MS.gene06909	PPI
MS.gene06696	MS.gene059160	PPI
MS.gene059160	MS.gene059161	PPI
MS.gene059160	MS.gene54540	PPI
MS.gene059160	MS.gene06696	PPI
MS.gene059160	MS.gene06907	PPI
MS.gene059160	MS.gene021450	PPI
MS.gene059160	MS.gene013579	PPI
MS.gene059160	MS.gene059162	PPI
MS.gene059162	MS.gene059160	PPI
MS.gene057114	MS.gene059160	PPI
MS.gene059160	MS.gene31640	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059160.t1	MTR_1g007905	91.005	189	16	1	1	189	1	188	4.52e-123	346
MS.gene059160.t1	MTR_3g091680	55.844	154	53	5	37	189	1	140	6.26e-44	144

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059160.t1	AT5G53030	38.333	180	70	9	45	189	40	213	1.70e-19	82.8
MS.gene059160.t1	AT5G53030	38.333	180	70	9	45	189	40	213	1.94e-19	83.2
MS.gene059160.t1	AT4G27810	35.176	199	78	11	9	188	1	167	5.51e-11	59.3

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGGCAGTGTGAATTT+TGG	0.173203	1.2:+12138327	MS.gene059160:CDS
TCCTCCTAGGTTGTTGTCTT+TGG	0.204117	1.2:+12138197	MS.gene059160:CDS
TTGTTACCTTCTCTTTCTTT+TGG	0.308850	1.2:-12138153	None:intergenic
ACCATCACCAACAACTGTTT+TGG	0.363972	1.2:+12138248	MS.gene059160:CDS
TAACAACGTTGTTAGAGCTT+TGG	0.381783	1.2:+12138170	MS.gene059160:CDS
CACATGGTACTTCACATTTG+TGG	0.395630	1.2:+12138481	MS.gene059160:CDS
TTGGGTCTAGTAGGTGGGGT+GGG	0.401963	1.2:+12138346	MS.gene059160:CDS
ATCATGATGATGATGAATTA+AGG	0.404054	1.2:+12137965	MS.gene059160:CDS
ACATGGTACTTCACATTTGT+GGG	0.406088	1.2:+12138482	MS.gene059160:CDS
ATAGCCGTCGTTCGCGGTGG+TGG	0.414893	1.2:-12138066	None:intergenic
TTTCAAGTTGGAGTGTTGAT+GGG	0.452572	1.2:+12138412	MS.gene059160:CDS
TTTAAGTGGGAGGAAGCACC+AGG	0.462547	1.2:+12138099	MS.gene059160:CDS
GTCGTTCGCGGTGGTGGAGT+TGG	0.473237	1.2:-12138060	None:intergenic
TAGGTTGTTGTCTTTGGAGA+GGG	0.474181	1.2:+12138203	MS.gene059160:CDS
GGACCTTCCAAAACAGTTGT+TGG	0.481823	1.2:-12138255	None:intergenic
TGACATGACCTAGGCTTCCC+TGG	0.485647	1.2:-12138117	None:intergenic
GGTGCTTCCTCCCACTTAAA+CGG	0.493829	1.2:-12138096	None:intergenic
GAGCTTTGGAACTTCCTCCT+AGG	0.498331	1.2:+12138184	MS.gene059160:CDS
TTTGGGTCTAGTAGGTGGGG+TGG	0.500229	1.2:+12138345	MS.gene059160:CDS
GACACTGCACGTCCAACGTA+TGG	0.515972	1.2:-12138276	None:intergenic
GATCACAAGAGTTAAGAGAA+GGG	0.526664	1.2:+12138449	MS.gene059160:CDS
CTAGGTTGTTGTCTTTGGAG+AGG	0.533267	1.2:+12138202	MS.gene059160:CDS
TTCAAGTTGGAGTGTTGATG+GGG	0.539072	1.2:+12138413	MS.gene059160:CDS
CTATATCGATACCGTTTAAG+TGG	0.539738	1.2:+12138085	MS.gene059160:CDS
AACTCCACCACCGCGAACGA+CGG	0.539846	1.2:+12138062	MS.gene059160:CDS
AGGAAGCACCAGGGAAGCCT+AGG	0.540552	1.2:+12138109	MS.gene059160:CDS
ATCGATATAGCCGTCGTTCG+CGG	0.559277	1.2:-12138072	None:intergenic
AGATCACAAGAGTTAAGAGA+AGG	0.565850	1.2:+12138448	MS.gene059160:CDS
ATCACAAGAGTTAAGAGAAG+GGG	0.589130	1.2:+12138450	MS.gene059160:CDS
TCCAAAACAGTTGTTGGTGA+TGG	0.590521	1.2:-12138249	None:intergenic
ATCGATACCGTTTAAGTGGG+AGG	0.600972	1.2:+12138089	MS.gene059160:CDS
CCAGAGCCAAAAGAAAGAGA+AGG	0.615358	1.2:+12138147	MS.gene059160:CDS
GTATAGGGATAGTAATAACA+AGG	0.626999	1.2:+12138311	MS.gene059160:CDS
CACGGTCGTGATAACACTGG+TGG	0.632454	1.2:-12138021	None:intergenic
TCCAAAGACAACAACCTAGG+AGG	0.639929	1.2:-12138198	None:intergenic
GGTCGTGTATGACATGACCT+AGG	0.639981	1.2:-12138126	None:intergenic
TATATCGATACCGTTTAAGT+GGG	0.641926	1.2:+12138086	MS.gene059160:CDS
TCAAGTTGGAGTGTTGATGG+GGG	0.642096	1.2:+12138414	MS.gene059160:CDS
AAAAGTCAAAACTACCCTCA+CGG	0.642581	1.2:-12138392	None:intergenic
AATAATAGAATTGACCGTGA+GGG	0.656653	1.2:+12138378	MS.gene059160:CDS
CTCTCCAAAGACAACAACCT+AGG	0.661580	1.2:-12138201	None:intergenic
AAACACGGTCGTGATAACAC+TGG	0.682213	1.2:-12138024	None:intergenic
AAATAATAGAATTGACCGTG+AGG	0.684752	1.2:+12138377	MS.gene059160:CDS
GATATAGCCGTCGTTCGCGG+TGG	0.697862	1.2:-12138069	None:intergenic
TATAGGGATAGTAATAACAA+GGG	0.698970	1.2:+12138312	MS.gene059160:CDS
AGAAGGGGAAGCTTCTCACA+TGG	0.717228	1.2:+12138465	MS.gene059160:CDS
TTAAGTGGGAGGAAGCACCA+GGG	0.769568	1.2:+12138100	MS.gene059160:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATAGGGATAGTAATAACAA+GGG	+	chr1.2:12138312-12138331	MS.gene059160:CDS	25.0%
!!	ATCATGATGATGATGAATTA+AGG	+	chr1.2:12137965-12137984	MS.gene059160:CDS	25.0%
	AAATAATAGAATTGACCGTG+AGG	+	chr1.2:12138377-12138396	MS.gene059160:CDS	30.0%
	AATAATAGAATTGACCGTGA+GGG	+	chr1.2:12138378-12138397	MS.gene059160:CDS	30.0%
	GTATAGGGATAGTAATAACA+AGG	+	chr1.2:12138311-12138330	MS.gene059160:CDS	30.0%
	TATATCGATACCGTTTAAGT+GGG	+	chr1.2:12138086-12138105	MS.gene059160:CDS	30.0%
!	TTGTTACCTTCTCTTTCTTT+TGG	-	chr1.2:12138156-12138175	None:intergenic	30.0%
!!!	GTAGTTTTGACTTTTCAAGT+TGG	+	chr1.2:12138400-12138419	MS.gene059160:CDS	30.0%
	AAAAGTCAAAACTACCCTCA+CGG	-	chr1.2:12138395-12138414	None:intergenic	35.0%
	AGATCACAAGAGTTAAGAGA+AGG	+	chr1.2:12138448-12138467	MS.gene059160:CDS	35.0%
	ATCACAAGAGTTAAGAGAAG+GGG	+	chr1.2:12138450-12138469	MS.gene059160:CDS	35.0%
	CTATATCGATACCGTTTAAG+TGG	+	chr1.2:12138085-12138104	MS.gene059160:CDS	35.0%
	GATCACAAGAGTTAAGAGAA+GGG	+	chr1.2:12138449-12138468	MS.gene059160:CDS	35.0%
	TAACAACGTTGTTAGAGCTT+TGG	+	chr1.2:12138170-12138189	MS.gene059160:CDS	35.0%
!!	TTTCAAGTTGGAGTGTTGAT+GGG	+	chr1.2:12138412-12138431	MS.gene059160:CDS	35.0%
!!!	TTTTCAAGTTGGAGTGTTGA+TGG	+	chr1.2:12138411-12138430	MS.gene059160:CDS	35.0%
	CACATGGTACTTCACATTTG+TGG	+	chr1.2:12138481-12138500	MS.gene059160:CDS	40.0%
!	AACAAGGGCAGTGTGAATTT+TGG	+	chr1.2:12138327-12138346	MS.gene059160:CDS	40.0%
!	ACAAGGGCAGTGTGAATTTT+GGG	+	chr1.2:12138328-12138347	MS.gene059160:CDS	40.0%
!	ACCATCACCAACAACTGTTT+TGG	+	chr1.2:12138248-12138267	MS.gene059160:CDS	40.0%
!	GGTGGAAGAGAAAGTTTTTG+TGG	-	chr1.2:12138006-12138025	None:intergenic	40.0%
!	GTCTTTTACGACGTCGTATA+GGG	+	chr1.2:12138296-12138315	MS.gene059160:CDS	40.0%
!	TAGGTTGTTGTCTTTGGAGA+GGG	+	chr1.2:12138203-12138222	MS.gene059160:CDS	40.0%
!	TCCAAAACAGTTGTTGGTGA+TGG	-	chr1.2:12138252-12138271	None:intergenic	40.0%
!	TGTCTTTTACGACGTCGTAT+AGG	+	chr1.2:12138295-12138314	MS.gene059160:CDS	40.0%
!!	AATTTTGGGTCTAGTAGGTG+GGG	+	chr1.2:12138342-12138361	MS.gene059160:CDS	40.0%
!!	GAATTTTGGGTCTAGTAGGT+GGG	+	chr1.2:12138341-12138360	MS.gene059160:CDS	40.0%
!!	GTGTGAATTTTGGGTCTAGT+AGG	+	chr1.2:12138337-12138356	MS.gene059160:CDS	40.0%
!!	TCACCAACAACTGTTTTGGA+AGG	+	chr1.2:12138252-12138271	MS.gene059160:CDS	40.0%
!!	TGAATTTTGGGTCTAGTAGG+TGG	+	chr1.2:12138340-12138359	MS.gene059160:CDS	40.0%
!!	TTCAAGTTGGAGTGTTGATG+GGG	+	chr1.2:12138413-12138432	MS.gene059160:CDS	40.0%
	AAACACGGTCGTGATAACAC+TGG	-	chr1.2:12138027-12138046	None:intergenic	45.0%
	ATCGATACCGTTTAAGTGGG+AGG	+	chr1.2:12138089-12138108	MS.gene059160:CDS	45.0%
	CCAGAGCCAAAAGAAAGAGA+AGG	+	chr1.2:12138147-12138166	MS.gene059160:CDS	45.0%
	CTCTCCAAAGACAACAACCT+AGG	-	chr1.2:12138204-12138223	None:intergenic	45.0%
	GGACCTTCCAAAACAGTTGT+TGG	-	chr1.2:12138258-12138277	None:intergenic	45.0%
	TCCAAAGACAACAACCTAGG+AGG	-	chr1.2:12138201-12138220	None:intergenic	45.0%
	TCCTCCTAGGTTGTTGTCTT+TGG	+	chr1.2:12138197-12138216	MS.gene059160:CDS	45.0%
!	ATCACGACCGTGTTTTTCGT+CGG	+	chr1.2:12138032-12138051	MS.gene059160:CDS	45.0%
!	CTAGGTTGTTGTCTTTGGAG+AGG	+	chr1.2:12138202-12138221	MS.gene059160:CDS	45.0%
!!	TCAAGTTGGAGTGTTGATGG+GGG	+	chr1.2:12138414-12138433	MS.gene059160:CDS	45.0%
!!!	CCTTCTCTTTCTTTTGGCTC+TGG	-	chr1.2:12138150-12138169	None:intergenic	45.0%
!!!	GTTTTGGAAGGTCCATACGT+TGG	+	chr1.2:12138264-12138283	MS.gene059160:CDS	45.0%
	AGAAGGGGAAGCTTCTCACA+TGG	+	chr1.2:12138465-12138484	MS.gene059160:CDS	50.0%
	ATCGATATAGCCGTCGTTCG+CGG	-	chr1.2:12138075-12138094	None:intergenic	50.0%
	GGTCGTGTATGACATGACCT+AGG	-	chr1.2:12138129-12138148	None:intergenic	50.0%
	GGTGCTTCCTCCCACTTAAA+CGG	-	chr1.2:12138099-12138118	None:intergenic	50.0%
!!	GAGCTTTGGAACTTCCTCCT+AGG	+	chr1.2:12138184-12138203	MS.gene059160:CDS	50.0%
!!	GAGTGTTGATGGGGGTGATA+AGG	+	chr1.2:12138422-12138441	MS.gene059160:CDS	50.0%
!!	TTAAGTGGGAGGAAGCACCA+GGG	+	chr1.2:12138100-12138119	MS.gene059160:CDS	50.0%
!!	TTTAAGTGGGAGGAAGCACC+AGG	+	chr1.2:12138099-12138118	MS.gene059160:CDS	50.0%
	CACGGTCGTGATAACACTGG+TGG	-	chr1.2:12138024-12138043	None:intergenic	55.0%
	GACACTGCACGTCCAACGTA+TGG	-	chr1.2:12138279-12138298	None:intergenic	55.0%
	GGTGCCTCCGACGAAAAACA+CGG	-	chr1.2:12138042-12138061	None:intergenic	55.0%
	TGACATGACCTAGGCTTCCC+TGG	-	chr1.2:12138120-12138139	None:intergenic	55.0%
	TTGGGTCTAGTAGGTGGGGT+GGG	+	chr1.2:12138346-12138365	MS.gene059160:CDS	55.0%
	TTTGGGTCTAGTAGGTGGGG+TGG	+	chr1.2:12138345-12138364	MS.gene059160:CDS	55.0%
!	ACGACCGTGTTTTTCGTCGG+AGG	+	chr1.2:12138035-12138054	MS.gene059160:CDS	55.0%
	AACTCCACCACCGCGAACGA+CGG	+	chr1.2:12138062-12138081	MS.gene059160:CDS	60.0%
	GATATAGCCGTCGTTCGCGG+TGG	-	chr1.2:12138072-12138091	None:intergenic	60.0%
!!	AGGAAGCACCAGGGAAGCCT+AGG	+	chr1.2:12138109-12138128	MS.gene059160:CDS	60.0%
	ATAGCCGTCGTTCGCGGTGG+TGG	-	chr1.2:12138069-12138088	None:intergenic	65.0%
!!	GTCGTTCGCGGTGGTGGAGT+TGG	-	chr1.2:12138063-12138082	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	12137937	12138503	12137937	ID=MS.gene059160
chr1.2	mRNA	12137937	12138503	12137937	ID=MS.gene059160.t1;Parent=MS.gene059160
chr1.2	exon	12137937	12138503	12137937	ID=MS.gene059160.t1.exon1;Parent=MS.gene059160.t1
chr1.2	CDS	12137937	12138503	12137937	ID=cds.MS.gene059160.t1;Parent=MS.gene059160.t1

Gene Sequence

>MS.gene059160

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGAGCAACTACAACACACCACAAAAACTTTCTCTTCCACCAGTGTTATCACGACCGTGTTTTTCGTCGGAGGCACCAACTCCACCACCGCGAACGACGGCTATATCGATACCGTTTAAGTGGGAGGAAGCACCAGGGAAGCCTAGGTCATGTCATACACGACCAGAGCCAAAAGAAAGAGAAGGTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCACCATCACCAACAACTGTTTTGGAAGGTCCATACGTTGGACGTGCAGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACAAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGG

Protein sequence

>MS.gene059160.t1

MTSENNKKLDHDDDELRSNYNTPQKLSLPPVLSRPCFSSEAPTPPPRTTAISIPFKWEEAPGKPRSCHTRPEPKEREGNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLW