AlfalfaGEDB | MS.gene31640

M. sativa cultivar XinJiangDaYe / MS.gene31640

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31640.t1	XP_013465692.1	91	189	16	1	1	189	1	188	2.20E-90	341.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31640.t1	A0A072VE41	91.0	189	16	1	1	189	1	188	1.6e-90	341.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene06909	MS.gene31640	PPI
MS.gene021450	MS.gene31640	PPI
MS.gene06907	MS.gene31640	PPI
MS.gene54540	MS.gene31640	PPI
MS.gene059162	MS.gene31640	PPI
MS.gene057114	MS.gene31640	PPI
MS.gene059161	MS.gene31640	PPI
MS.gene013579	MS.gene31640	PPI
MS.gene059160	MS.gene31640	PPI
MS.gene057116	MS.gene31640	PPI
MS.gene06696	MS.gene31640	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31640.t1	MTR_1g007905	90.526	190	17	1	1	190	1	189	1.61e-122	345
MS.gene31640.t1	MTR_3g091680	56.129	155	53	5	37	190	1	141	4.76e-45	147

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene31640.t1	AT5G53030	38.333	180	70	9	45	189	40	213	7.49e-20	84.0
MS.gene31640.t1	AT5G53030	38.333	180	70	9	45	189	40	213	8.83e-20	84.0
MS.gene31640.t1	AT4G27810	34.010	197	87	9	9	190	1	169	3.42e-11	60.1

Find 49 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGGCAGTGTGAATTT+TGG	0.173203	1.1:+11667083	MS.gene31640:CDS
TCCTCCTAGGTTGTTGTCTT+TGG	0.204117	1.1:+11666953	MS.gene31640:CDS
TTGTTACCTTCTCTTTCTTT+TGG	0.308850	1.1:-11666909	None:intergenic
GCCATCACCAACAACGGTTT+TGG	0.333830	1.1:+11667004	MS.gene31640:CDS
TAACAACGTTGTTAGAGCTT+TGG	0.381783	1.1:+11666926	MS.gene31640:CDS
CACATGGTACTTCACATTTG+TGG	0.395630	1.1:+11667237	MS.gene31640:CDS
TTGGGTCTAGTAGGTGGGGT+GGG	0.401963	1.1:+11667102	MS.gene31640:CDS
ATCATGATGATGATGAATTA+AGG	0.404054	1.1:+11666721	MS.gene31640:CDS
ACATGGTACTTCACATTTGT+GGG	0.406088	1.1:+11667238	MS.gene31640:CDS
ATAGCCATCGTTCGCGGTGG+TGG	0.423263	1.1:-11666822	None:intergenic
TTTCAAGTTGGAGTGTTGAT+GGG	0.452572	1.1:+11667168	MS.gene31640:CDS
ATCGTTCGCGGTGGTGGAGT+TGG	0.456005	1.1:-11666816	None:intergenic
TTTAAGTGGGAGGAAGCACC+AGG	0.462547	1.1:+11666855	MS.gene31640:CDS
TAGGTTGTTGTCTTTGGAGA+GGG	0.474181	1.1:+11666959	MS.gene31640:CDS
GGACCTTCCAAAACCGTTGT+TGG	0.475855	1.1:-11667011	None:intergenic
TGACATGACCTAGGCTTCCC+TGG	0.485647	1.1:-11666873	None:intergenic
TCCAAAACCGTTGTTGGTGA+TGG	0.490839	1.1:-11667005	None:intergenic
GGTGCTTCCTCCCACTTAAA+CGG	0.493829	1.1:-11666852	None:intergenic
GAGCTTTGGAACTTCCTCCT+AGG	0.498331	1.1:+11666940	MS.gene31640:CDS
TTTGGGTCTAGTAGGTGGGG+TGG	0.500229	1.1:+11667101	MS.gene31640:CDS
CACGGCCGCGTTAACGTTGG+TGG	0.513259	1.1:-11666777	None:intergenic
GACACTGCACGTCCAACGTA+TGG	0.515972	1.1:-11667032	None:intergenic
ATCGATATAGCCATCGTTCG+CGG	0.521540	1.1:-11666828	None:intergenic
GATCACAAGAGTTAAGAGAA+GGG	0.526664	1.1:+11667205	MS.gene31640:CDS
CTAGGTTGTTGTCTTTGGAG+AGG	0.533267	1.1:+11666958	MS.gene31640:CDS
TTCAAGTTGGAGTGTTGATG+GGG	0.539072	1.1:+11667169	MS.gene31640:CDS
AGGAAGCACCAGGGAAGCCT+AGG	0.540552	1.1:+11666865	MS.gene31640:CDS
CTATATCGATACCGTTTAAG+TGG	0.542009	1.1:+11666841	MS.gene31640:CDS
AACTCCACCACCGCGAACGA+TGG	0.544877	1.1:+11666818	MS.gene31640:CDS
AGATCACAAGAGTTAAGAGA+AGG	0.565850	1.1:+11667204	MS.gene31640:CDS
ATCACAAGAGTTAAGAGAAG+GGG	0.589130	1.1:+11667206	MS.gene31640:CDS
ATCGATACCGTTTAAGTGGG+AGG	0.600972	1.1:+11666845	MS.gene31640:CDS
CCAGAGCCAAAAGAAAGAGA+AGG	0.615358	1.1:+11666903	MS.gene31640:CDS
GTATAGGGATAGTAATAACA+AGG	0.626999	1.1:+11667067	MS.gene31640:CDS
CTCTTCCACCAACGTTAACG+CGG	0.628332	1.1:+11666772	MS.gene31640:CDS
TTACGCGCCATCACCAACAA+CGG	0.631163	1.1:+11666998	MS.gene31640:CDS
TCCAAAGACAACAACCTAGG+AGG	0.639929	1.1:-11666954	None:intergenic
GGTCGTGTATGACATGACCT+AGG	0.639981	1.1:-11666882	None:intergenic
TCAAGTTGGAGTGTTGATGG+GGG	0.642096	1.1:+11667170	MS.gene31640:CDS
AAAAGTCAAAACTACCCTCA+CGG	0.642581	1.1:-11667148	None:intergenic
TATATCGATACCGTTTAAGT+GGG	0.643220	1.1:+11666842	MS.gene31640:CDS
AAACACGGCCGCGTTAACGT+TGG	0.653628	1.1:-11666780	None:intergenic
AATAATAGAATTGACCGTGA+GGG	0.656653	1.1:+11667134	MS.gene31640:CDS
CTCTCCAAAGACAACAACCT+AGG	0.661580	1.1:-11666957	None:intergenic
AAATAATAGAATTGACCGTG+AGG	0.684752	1.1:+11667133	MS.gene31640:CDS
TATAGGGATAGTAATAACAA+GGG	0.698970	1.1:+11667068	MS.gene31640:CDS
AGAAGGGGAAGCTTCTCACA+TGG	0.717228	1.1:+11667221	MS.gene31640:CDS
GATATAGCCATCGTTCGCGG+TGG	0.724077	1.1:-11666825	None:intergenic
TTAAGTGGGAGGAAGCACCA+GGG	0.769568	1.1:+11666856	MS.gene31640:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAGGTATATAAAATAAGAT+AGG	-	chr1.1:11667276-11667295	None:intergenic	15.0%
!	ATTGTAGTTTCAAATGCTTA+GGG	+	chr1.1:11667321-11667340	MS.gene31640:intron	25.0%
!	TATAGGGATAGTAATAACAA+GGG	+	chr1.1:11667068-11667087	MS.gene31640:CDS	25.0%
!!	ATCATGATGATGATGAATTA+AGG	+	chr1.1:11666721-11666740	MS.gene31640:CDS	25.0%
	AAATAATAGAATTGACCGTG+AGG	+	chr1.1:11667133-11667152	MS.gene31640:CDS	30.0%
	AATAATAGAATTGACCGTGA+GGG	+	chr1.1:11667134-11667153	MS.gene31640:CDS	30.0%
	GATTGTAGTTTCAAATGCTT+AGG	+	chr1.1:11667320-11667339	MS.gene31640:intron	30.0%
	GTATAGGGATAGTAATAACA+AGG	+	chr1.1:11667067-11667086	MS.gene31640:CDS	30.0%
	TATATCGATACCGTTTAAGT+GGG	+	chr1.1:11666842-11666861	MS.gene31640:CDS	30.0%
!	TTGTTACCTTCTCTTTCTTT+TGG	-	chr1.1:11666912-11666931	None:intergenic	30.0%
!!!	GTAGTTTTGACTTTTCAAGT+TGG	+	chr1.1:11667156-11667175	MS.gene31640:CDS	30.0%
	AAAAGTCAAAACTACCCTCA+CGG	-	chr1.1:11667151-11667170	None:intergenic	35.0%
	ACATGGTACTTCACATTTGT+GGG	+	chr1.1:11667238-11667257	MS.gene31640:CDS	35.0%
	AGATCACAAGAGTTAAGAGA+AGG	+	chr1.1:11667204-11667223	MS.gene31640:CDS	35.0%
	ATCACAAGAGTTAAGAGAAG+GGG	+	chr1.1:11667206-11667225	MS.gene31640:CDS	35.0%
	CTATATCGATACCGTTTAAG+TGG	+	chr1.1:11666841-11666860	MS.gene31640:CDS	35.0%
	GATCACAAGAGTTAAGAGAA+GGG	+	chr1.1:11667205-11667224	MS.gene31640:CDS	35.0%
	TAACAACGTTGTTAGAGCTT+TGG	+	chr1.1:11666926-11666945	MS.gene31640:CDS	35.0%
!!	TTTCAAGTTGGAGTGTTGAT+GGG	+	chr1.1:11667168-11667187	MS.gene31640:CDS	35.0%
!!!	TTTTCAAGTTGGAGTGTTGA+TGG	+	chr1.1:11667167-11667186	MS.gene31640:CDS	35.0%
	CACATGGTACTTCACATTTG+TGG	+	chr1.1:11667237-11667256	MS.gene31640:CDS	40.0%
	CATGAAGAGTGAGACATGAA+TGG	-	chr1.1:11667300-11667319	None:intergenic	40.0%
	CATTCATGTCTCACTCTTCA+TGG	+	chr1.1:11667298-11667317	MS.gene31640:intron	40.0%
	GAGTGAGACATGAATGGATA+AGG	-	chr1.1:11667294-11667313	None:intergenic	40.0%
!	AACAAGGGCAGTGTGAATTT+TGG	+	chr1.1:11667083-11667102	MS.gene31640:CDS	40.0%
!	ACAAGGGCAGTGTGAATTTT+GGG	+	chr1.1:11667084-11667103	MS.gene31640:CDS	40.0%
!	GGTGGAAGAGAAAGTTTTTG+TGG	-	chr1.1:11666762-11666781	None:intergenic	40.0%
!	GTCTTTTACGACGTCGTATA+GGG	+	chr1.1:11667052-11667071	MS.gene31640:CDS	40.0%
!	TAGGTTGTTGTCTTTGGAGA+GGG	+	chr1.1:11666959-11666978	MS.gene31640:CDS	40.0%
!	TGTCTTTTACGACGTCGTAT+AGG	+	chr1.1:11667051-11667070	MS.gene31640:CDS	40.0%
!!	AATTTTGGGTCTAGTAGGTG+GGG	+	chr1.1:11667098-11667117	MS.gene31640:CDS	40.0%
!!	GAATTTTGGGTCTAGTAGGT+GGG	+	chr1.1:11667097-11667116	MS.gene31640:CDS	40.0%
!!	GTGTGAATTTTGGGTCTAGT+AGG	+	chr1.1:11667093-11667112	MS.gene31640:CDS	40.0%
!!	TGAATTTTGGGTCTAGTAGG+TGG	+	chr1.1:11667096-11667115	MS.gene31640:CDS	40.0%
!!	TTCAAGTTGGAGTGTTGATG+GGG	+	chr1.1:11667169-11667188	MS.gene31640:CDS	40.0%
	ATCGATACCGTTTAAGTGGG+AGG	+	chr1.1:11666845-11666864	MS.gene31640:CDS	45.0%
	ATCGATATAGCCATCGTTCG+CGG	-	chr1.1:11666831-11666850	None:intergenic	45.0%
	CCAGAGCCAAAAGAAAGAGA+AGG	+	chr1.1:11666903-11666922	MS.gene31640:CDS	45.0%
	CTCTCCAAAGACAACAACCT+AGG	-	chr1.1:11666960-11666979	None:intergenic	45.0%
	TCCAAAGACAACAACCTAGG+AGG	-	chr1.1:11666957-11666976	None:intergenic	45.0%
	TCCTCCTAGGTTGTTGTCTT+TGG	+	chr1.1:11666953-11666972	MS.gene31640:CDS	45.0%
!	CTAGGTTGTTGTCTTTGGAG+AGG	+	chr1.1:11666958-11666977	MS.gene31640:CDS	45.0%
!	TCCAAAACCGTTGTTGGTGA+TGG	-	chr1.1:11667008-11667027	None:intergenic	45.0%
!!	TCAAGTTGGAGTGTTGATGG+GGG	+	chr1.1:11667170-11667189	MS.gene31640:CDS	45.0%
!!!	CCTTCTCTTTCTTTTGGCTC+TGG	-	chr1.1:11666906-11666925	None:intergenic	45.0%
!!!	GTTTTGGAAGGTCCATACGT+TGG	+	chr1.1:11667020-11667039	MS.gene31640:CDS	45.0%
!!!	TCACCAACAACGGTTTTGGA+AGG	+	chr1.1:11667008-11667027	MS.gene31640:CDS	45.0%
	AGAAGGGGAAGCTTCTCACA+TGG	+	chr1.1:11667221-11667240	MS.gene31640:CDS	50.0%
	CTCTTCCACCAACGTTAACG+CGG	+	chr1.1:11666772-11666791	MS.gene31640:CDS	50.0%
	GGACCTTCCAAAACCGTTGT+TGG	-	chr1.1:11667014-11667033	None:intergenic	50.0%
	GGTCGTGTATGACATGACCT+AGG	-	chr1.1:11666885-11666904	None:intergenic	50.0%
	GGTGCTTCCTCCCACTTAAA+CGG	-	chr1.1:11666855-11666874	None:intergenic	50.0%
	TTACGCGCCATCACCAACAA+CGG	+	chr1.1:11666998-11667017	MS.gene31640:CDS	50.0%
!	GCCATCACCAACAACGGTTT+TGG	+	chr1.1:11667004-11667023	MS.gene31640:CDS	50.0%
!!	GAGCTTTGGAACTTCCTCCT+AGG	+	chr1.1:11666940-11666959	MS.gene31640:CDS	50.0%
!!	GAGTGTTGATGGGGGTGATA+AGG	+	chr1.1:11667178-11667197	MS.gene31640:CDS	50.0%
!!	TTAAGTGGGAGGAAGCACCA+GGG	+	chr1.1:11666856-11666875	MS.gene31640:CDS	50.0%
!!	TTTAAGTGGGAGGAAGCACC+AGG	+	chr1.1:11666855-11666874	MS.gene31640:CDS	50.0%
	AAACACGGCCGCGTTAACGT+TGG	-	chr1.1:11666783-11666802	None:intergenic	55.0%
	GACACTGCACGTCCAACGTA+TGG	-	chr1.1:11667035-11667054	None:intergenic	55.0%
	GATATAGCCATCGTTCGCGG+TGG	-	chr1.1:11666828-11666847	None:intergenic	55.0%
	GGTGCCTCCGACGAAAAACA+CGG	-	chr1.1:11666798-11666817	None:intergenic	55.0%
	TGACATGACCTAGGCTTCCC+TGG	-	chr1.1:11666876-11666895	None:intergenic	55.0%
	TTGGGTCTAGTAGGTGGGGT+GGG	+	chr1.1:11667102-11667121	MS.gene31640:CDS	55.0%
	TTTGGGTCTAGTAGGTGGGG+TGG	+	chr1.1:11667101-11667120	MS.gene31640:CDS	55.0%
!	AACGCGGCCGTGTTTTTCGT+CGG	+	chr1.1:11666788-11666807	MS.gene31640:CDS	55.0%
	AACTCCACCACCGCGAACGA+TGG	+	chr1.1:11666818-11666837	MS.gene31640:CDS	60.0%
	ATAGCCATCGTTCGCGGTGG+TGG	-	chr1.1:11666825-11666844	None:intergenic	60.0%
!!	AGGAAGCACCAGGGAAGCCT+AGG	+	chr1.1:11666865-11666884	MS.gene31640:CDS	60.0%
!!	ATCGTTCGCGGTGGTGGAGT+TGG	-	chr1.1:11666819-11666838	None:intergenic	60.0%
	CACGGCCGCGTTAACGTTGG+TGG	-	chr1.1:11666780-11666799	None:intergenic	65.0%
!	GCGGCCGTGTTTTTCGTCGG+AGG	+	chr1.1:11666791-11666810	MS.gene31640:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	11666693	11667347	11666693	ID=MS.gene31640
chr1.1	mRNA	11666693	11667347	11666693	ID=MS.gene31640.t1;Parent=MS.gene31640
chr1.1	exon	11666693	11667259	11666693	ID=MS.gene31640.t1.exon1;Parent=MS.gene31640.t1
chr1.1	CDS	11666693	11667259	11666693	ID=cds.MS.gene31640.t1;Parent=MS.gene31640.t1
chr1.1	exon	11667342	11667347	11667342	ID=MS.gene31640.t1.exon2;Parent=MS.gene31640.t1
chr1.1	CDS	11667342	11667347	11667342	ID=cds.MS.gene31640.t1;Parent=MS.gene31640.t1

Gene Sequence

>MS.gene31640

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGAGCAACTACAACACACCACAAAAACTTTCTCTTCCACCAACGTTAACGCGGCCGTGTTTTTCGTCGGAGGCACCAACTCCACCACCGCGAACGATGGCTATATCGATACCGTTTAAGTGGGAGGAAGCACCAGGGAAGCCTAGGTCATGTCATACACGACCAGAGCCAAAAGAAAGAGAAGGTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCGCCATCACCAACAACGGTTTTGGAAGGTCCATACGTTGGACGTGCAGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACAAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGGGTATGTCTCTTTTCCTATCTTATTTTATATACCTTATCCATTCATGTCTCACTCTTCATGGATTGTAGTTTCAAATGCTTAGGGATAG

Protein sequence

>MS.gene31640.t1

MTSENNKKLDHDDDELRSNYNTPQKLSLPPTLTRPCFSSEAPTPPPRTMAISIPFKWEEAPGKPRSCHTRPEPKEREGNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLWG