AlfalfaGEDB | MS.gene059162

M. sativa cultivar XinJiangDaYe / MS.gene059162

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059162.t1	XP_013465692.1	91	189	16	1	1	189	1	188	4.50E-91	344

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059162.t1	A0A072VE41	91.0	189	16	1	1	189	1	188	3.3e-91	344.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene053166	MS.gene059162	0.828234	1.01E-54	-1.69E-46
MS.gene055701	MS.gene059162	0.841793	3.83E-58	-1.69E-46
MS.gene057156	MS.gene059162	-0.808171	3.53E-50	-1.69E-46
MS.gene059160	MS.gene059162	0.989339	1.07E-177	-1.69E-46
MS.gene059161	MS.gene059162	0.931671	2.67E-94	-1.69E-46
MS.gene059162	MS.gene059609	0.815885	7.35E-52	-1.69E-46
MS.gene059162	MS.gene060802	0.81196	5.39E-51	-1.69E-46
MS.gene059162	MS.gene061506	0.813431	2.57E-51	-1.69E-46
MS.gene059162	MS.gene072375	-0.829319	5.50E-55	-1.69E-46
MS.gene059162	MS.gene074184	-0.826309	2.91E-54	-1.69E-46
MS.gene059162	MS.gene074393	-0.815158	1.07E-51	-1.69E-46
MS.gene059162	MS.gene20354	0.848376	6.38E-60	-1.69E-46
MS.gene059162	MS.gene25913	-0.806268	8.93E-50	-1.69E-46
MS.gene059162	MS.gene28532	0.808043	3.76E-50	-1.69E-46
MS.gene059162	MS.gene28862	0.801743	7.80E-49	-1.69E-46
MS.gene059162	MS.gene29000	0.811191	7.92E-51	-1.69E-46
MS.gene059162	MS.gene37086	0.831191	1.92E-55	-1.69E-46
MS.gene059162	MS.gene38381	0.80749	4.93E-50	-1.69E-46
MS.gene059162	MS.gene49565	0.809852	1.54E-50	-1.69E-46
MS.gene059162	MS.gene51548	-0.8115	6.79E-51	-1.69E-46
MS.gene059162	MS.gene53156	0.814232	1.71E-51	-1.69E-46
MS.gene059162	MS.gene54540	0.940344	2.74E-100	-1.69E-46
MS.gene059162	MS.gene58503	0.818755	1.66E-52	-1.69E-46
MS.gene059162	MS.gene59948	0.819901	9.10E-53	-1.69E-46
MS.gene059162	MS.gene61667	0.801479	8.83E-49	-1.69E-46
MS.gene059162	MS.gene66208	0.810038	1.41E-50	-1.69E-46
MS.gene059162	MS.gene68528	0.806978	6.33E-50	-1.69E-46
MS.gene059162	MS.gene70021	0.821207	4.56E-53	-1.69E-46
MS.gene059162	MS.gene70586	-0.808032	3.78E-50	-1.69E-46
MS.gene059162	MS.gene72054	0.800188	1.62E-48	-1.69E-46
MS.gene059162	MS.gene75598	-0.800419	1.45E-48	-1.69E-46
MS.gene059162	MS.gene87669	0.822281	2.58E-53	-1.69E-46
MS.gene059162	MS.gene95377	0.801991	6.94E-49	-1.69E-46
MS.gene059162	MS.gene99702	0.801531	8.62E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene059162	MS.gene06696	PPI
MS.gene059162	MS.gene06907	PPI
MS.gene059162	MS.gene021450	PPI
MS.gene059162	MS.gene013579	PPI
MS.gene057114	MS.gene059162	PPI
MS.gene059162	MS.gene31640	PPI
MS.gene013579	MS.gene059162	PPI
MS.gene059160	MS.gene059162	PPI
MS.gene057116	MS.gene059162	PPI
MS.gene059162	MS.gene059160	PPI
MS.gene059162	MS.gene057116	PPI
MS.gene06696	MS.gene059162	PPI
MS.gene059162	MS.gene057114	PPI
MS.gene059162	MS.gene06909	PPI
MS.gene059162	MS.gene059161	PPI
MS.gene059162	MS.gene54540	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059162.t1	MTR_1g007905	91.005	189	16	1	1	189	1	188	4.52e-123	346
MS.gene059162.t1	MTR_3g091680	55.844	154	53	5	37	189	1	140	6.26e-44	144

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059162.t1	AT5G53030	38.333	180	70	9	45	189	40	213	1.70e-19	82.8
MS.gene059162.t1	AT5G53030	38.333	180	70	9	45	189	40	213	1.94e-19	83.2
MS.gene059162.t1	AT4G27810	35.176	199	78	11	9	188	1	167	5.51e-11	59.3

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAGGGCAGTGTGAATTT+TGG	0.173203	1.2:+12165445	MS.gene059162:CDS
TCCTCCTAGGTTGTTGTCTT+TGG	0.204117	1.2:+12165315	MS.gene059162:CDS
TTGTTACCTTCTCTTTCTTT+TGG	0.308850	1.2:-12165271	None:intergenic
ACCATCACCAACAACTGTTT+TGG	0.363972	1.2:+12165366	MS.gene059162:CDS
TAACAACGTTGTTAGAGCTT+TGG	0.381783	1.2:+12165288	MS.gene059162:CDS
CACATGGTACTTCACATTTG+TGG	0.395630	1.2:+12165599	MS.gene059162:CDS
TTGGGTCTAGTAGGTGGGGT+GGG	0.401963	1.2:+12165464	MS.gene059162:CDS
ATCATGATGATGATGAATTA+AGG	0.404054	1.2:+12165083	MS.gene059162:CDS
ACATGGTACTTCACATTTGT+GGG	0.406088	1.2:+12165600	MS.gene059162:CDS
ATAGCCGTCGTTCGCGGTGG+TGG	0.414893	1.2:-12165184	None:intergenic
TTTCAAGTTGGAGTGTTGAT+GGG	0.452572	1.2:+12165530	MS.gene059162:CDS
TTTAAGTGGGAGGAAGCACC+AGG	0.462547	1.2:+12165217	MS.gene059162:CDS
GTCGTTCGCGGTGGTGGAGT+TGG	0.473237	1.2:-12165178	None:intergenic
TAGGTTGTTGTCTTTGGAGA+GGG	0.474181	1.2:+12165321	MS.gene059162:CDS
GGACCTTCCAAAACAGTTGT+TGG	0.481823	1.2:-12165373	None:intergenic
TGACATGACCTAGGCTTCCC+TGG	0.485647	1.2:-12165235	None:intergenic
GGTGCTTCCTCCCACTTAAA+CGG	0.493829	1.2:-12165214	None:intergenic
GAGCTTTGGAACTTCCTCCT+AGG	0.498331	1.2:+12165302	MS.gene059162:CDS
TTTGGGTCTAGTAGGTGGGG+TGG	0.500229	1.2:+12165463	MS.gene059162:CDS
GACACTGCACGTCCAACGTA+TGG	0.515972	1.2:-12165394	None:intergenic
GATCACAAGAGTTAAGAGAA+GGG	0.526664	1.2:+12165567	MS.gene059162:CDS
CTAGGTTGTTGTCTTTGGAG+AGG	0.533267	1.2:+12165320	MS.gene059162:CDS
TTCAAGTTGGAGTGTTGATG+GGG	0.539072	1.2:+12165531	MS.gene059162:CDS
CTATATCGATACCGTTTAAG+TGG	0.539738	1.2:+12165203	MS.gene059162:CDS
AACTCCACCACCGCGAACGA+CGG	0.539846	1.2:+12165180	MS.gene059162:CDS
AGGAAGCACCAGGGAAGCCT+AGG	0.540552	1.2:+12165227	MS.gene059162:CDS
ATCGATATAGCCGTCGTTCG+CGG	0.559277	1.2:-12165190	None:intergenic
AGATCACAAGAGTTAAGAGA+AGG	0.565850	1.2:+12165566	MS.gene059162:CDS
ATCACAAGAGTTAAGAGAAG+GGG	0.589130	1.2:+12165568	MS.gene059162:CDS
TCCAAAACAGTTGTTGGTGA+TGG	0.590521	1.2:-12165367	None:intergenic
ATCGATACCGTTTAAGTGGG+AGG	0.600972	1.2:+12165207	MS.gene059162:CDS
CCAGAGCCAAAAGAAAGAGA+AGG	0.615358	1.2:+12165265	MS.gene059162:CDS
GTATAGGGATAGTAATAACA+AGG	0.626999	1.2:+12165429	MS.gene059162:CDS
CACGGTCGTGATAACACTGG+TGG	0.632454	1.2:-12165139	None:intergenic
TCCAAAGACAACAACCTAGG+AGG	0.639929	1.2:-12165316	None:intergenic
GGTCGTGTATGACATGACCT+AGG	0.639981	1.2:-12165244	None:intergenic
TATATCGATACCGTTTAAGT+GGG	0.641926	1.2:+12165204	MS.gene059162:CDS
TCAAGTTGGAGTGTTGATGG+GGG	0.642096	1.2:+12165532	MS.gene059162:CDS
AAAAGTCAAAACTACCCTCA+CGG	0.642581	1.2:-12165510	None:intergenic
AATAATAGAATTGACCGTGA+GGG	0.656653	1.2:+12165496	MS.gene059162:CDS
CTCTCCAAAGACAACAACCT+AGG	0.661580	1.2:-12165319	None:intergenic
AAACACGGTCGTGATAACAC+TGG	0.682213	1.2:-12165142	None:intergenic
AAATAATAGAATTGACCGTG+AGG	0.684752	1.2:+12165495	MS.gene059162:CDS
GATATAGCCGTCGTTCGCGG+TGG	0.697862	1.2:-12165187	None:intergenic
TATAGGGATAGTAATAACAA+GGG	0.698970	1.2:+12165430	MS.gene059162:CDS
AGAAGGGGAAGCTTCTCACA+TGG	0.717228	1.2:+12165583	MS.gene059162:CDS
TTAAGTGGGAGGAAGCACCA+GGG	0.769568	1.2:+12165218	MS.gene059162:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATAGGGATAGTAATAACAA+GGG	+	chr1.2:12165430-12165449	MS.gene059162:CDS	25.0%
!!	ATCATGATGATGATGAATTA+AGG	+	chr1.2:12165083-12165102	MS.gene059162:CDS	25.0%
	AAATAATAGAATTGACCGTG+AGG	+	chr1.2:12165495-12165514	MS.gene059162:CDS	30.0%
	AATAATAGAATTGACCGTGA+GGG	+	chr1.2:12165496-12165515	MS.gene059162:CDS	30.0%
	GTATAGGGATAGTAATAACA+AGG	+	chr1.2:12165429-12165448	MS.gene059162:CDS	30.0%
	TATATCGATACCGTTTAAGT+GGG	+	chr1.2:12165204-12165223	MS.gene059162:CDS	30.0%
!	TTGTTACCTTCTCTTTCTTT+TGG	-	chr1.2:12165274-12165293	None:intergenic	30.0%
!!!	GTAGTTTTGACTTTTCAAGT+TGG	+	chr1.2:12165518-12165537	MS.gene059162:CDS	30.0%
	AAAAGTCAAAACTACCCTCA+CGG	-	chr1.2:12165513-12165532	None:intergenic	35.0%
	AGATCACAAGAGTTAAGAGA+AGG	+	chr1.2:12165566-12165585	MS.gene059162:CDS	35.0%
	ATCACAAGAGTTAAGAGAAG+GGG	+	chr1.2:12165568-12165587	MS.gene059162:CDS	35.0%
	CTATATCGATACCGTTTAAG+TGG	+	chr1.2:12165203-12165222	MS.gene059162:CDS	35.0%
	GATCACAAGAGTTAAGAGAA+GGG	+	chr1.2:12165567-12165586	MS.gene059162:CDS	35.0%
	TAACAACGTTGTTAGAGCTT+TGG	+	chr1.2:12165288-12165307	MS.gene059162:CDS	35.0%
!!	TTTCAAGTTGGAGTGTTGAT+GGG	+	chr1.2:12165530-12165549	MS.gene059162:CDS	35.0%
!!!	TTTTCAAGTTGGAGTGTTGA+TGG	+	chr1.2:12165529-12165548	MS.gene059162:CDS	35.0%
	CACATGGTACTTCACATTTG+TGG	+	chr1.2:12165599-12165618	MS.gene059162:CDS	40.0%
!	AACAAGGGCAGTGTGAATTT+TGG	+	chr1.2:12165445-12165464	MS.gene059162:CDS	40.0%
!	ACAAGGGCAGTGTGAATTTT+GGG	+	chr1.2:12165446-12165465	MS.gene059162:CDS	40.0%
!	ACCATCACCAACAACTGTTT+TGG	+	chr1.2:12165366-12165385	MS.gene059162:CDS	40.0%
!	GGTGGAAGAGAAAGTTTTTG+TGG	-	chr1.2:12165124-12165143	None:intergenic	40.0%
!	GTCTTTTACGACGTCGTATA+GGG	+	chr1.2:12165414-12165433	MS.gene059162:CDS	40.0%
!	TAGGTTGTTGTCTTTGGAGA+GGG	+	chr1.2:12165321-12165340	MS.gene059162:CDS	40.0%
!	TCCAAAACAGTTGTTGGTGA+TGG	-	chr1.2:12165370-12165389	None:intergenic	40.0%
!	TGTCTTTTACGACGTCGTAT+AGG	+	chr1.2:12165413-12165432	MS.gene059162:CDS	40.0%
!!	AATTTTGGGTCTAGTAGGTG+GGG	+	chr1.2:12165460-12165479	MS.gene059162:CDS	40.0%
!!	GAATTTTGGGTCTAGTAGGT+GGG	+	chr1.2:12165459-12165478	MS.gene059162:CDS	40.0%
!!	GTGTGAATTTTGGGTCTAGT+AGG	+	chr1.2:12165455-12165474	MS.gene059162:CDS	40.0%
!!	TCACCAACAACTGTTTTGGA+AGG	+	chr1.2:12165370-12165389	MS.gene059162:CDS	40.0%
!!	TGAATTTTGGGTCTAGTAGG+TGG	+	chr1.2:12165458-12165477	MS.gene059162:CDS	40.0%
!!	TTCAAGTTGGAGTGTTGATG+GGG	+	chr1.2:12165531-12165550	MS.gene059162:CDS	40.0%
	AAACACGGTCGTGATAACAC+TGG	-	chr1.2:12165145-12165164	None:intergenic	45.0%
	ATCGATACCGTTTAAGTGGG+AGG	+	chr1.2:12165207-12165226	MS.gene059162:CDS	45.0%
	CCAGAGCCAAAAGAAAGAGA+AGG	+	chr1.2:12165265-12165284	MS.gene059162:CDS	45.0%
	CTCTCCAAAGACAACAACCT+AGG	-	chr1.2:12165322-12165341	None:intergenic	45.0%
	GGACCTTCCAAAACAGTTGT+TGG	-	chr1.2:12165376-12165395	None:intergenic	45.0%
	TCCAAAGACAACAACCTAGG+AGG	-	chr1.2:12165319-12165338	None:intergenic	45.0%
	TCCTCCTAGGTTGTTGTCTT+TGG	+	chr1.2:12165315-12165334	MS.gene059162:CDS	45.0%
!	ATCACGACCGTGTTTTTCGT+CGG	+	chr1.2:12165150-12165169	MS.gene059162:CDS	45.0%
!	CTAGGTTGTTGTCTTTGGAG+AGG	+	chr1.2:12165320-12165339	MS.gene059162:CDS	45.0%
!!	TCAAGTTGGAGTGTTGATGG+GGG	+	chr1.2:12165532-12165551	MS.gene059162:CDS	45.0%
!!!	CCTTCTCTTTCTTTTGGCTC+TGG	-	chr1.2:12165268-12165287	None:intergenic	45.0%
!!!	GTTTTGGAAGGTCCATACGT+TGG	+	chr1.2:12165382-12165401	MS.gene059162:CDS	45.0%
	AGAAGGGGAAGCTTCTCACA+TGG	+	chr1.2:12165583-12165602	MS.gene059162:CDS	50.0%
	ATCGATATAGCCGTCGTTCG+CGG	-	chr1.2:12165193-12165212	None:intergenic	50.0%
	GGTCGTGTATGACATGACCT+AGG	-	chr1.2:12165247-12165266	None:intergenic	50.0%
	GGTGCTTCCTCCCACTTAAA+CGG	-	chr1.2:12165217-12165236	None:intergenic	50.0%
!!	GAGCTTTGGAACTTCCTCCT+AGG	+	chr1.2:12165302-12165321	MS.gene059162:CDS	50.0%
!!	GAGTGTTGATGGGGGTGATA+AGG	+	chr1.2:12165540-12165559	MS.gene059162:CDS	50.0%
!!	TTAAGTGGGAGGAAGCACCA+GGG	+	chr1.2:12165218-12165237	MS.gene059162:CDS	50.0%
!!	TTTAAGTGGGAGGAAGCACC+AGG	+	chr1.2:12165217-12165236	MS.gene059162:CDS	50.0%
	CACGGTCGTGATAACACTGG+TGG	-	chr1.2:12165142-12165161	None:intergenic	55.0%
	GACACTGCACGTCCAACGTA+TGG	-	chr1.2:12165397-12165416	None:intergenic	55.0%
	GGTGCCTCCGACGAAAAACA+CGG	-	chr1.2:12165160-12165179	None:intergenic	55.0%
	TGACATGACCTAGGCTTCCC+TGG	-	chr1.2:12165238-12165257	None:intergenic	55.0%
	TTGGGTCTAGTAGGTGGGGT+GGG	+	chr1.2:12165464-12165483	MS.gene059162:CDS	55.0%
	TTTGGGTCTAGTAGGTGGGG+TGG	+	chr1.2:12165463-12165482	MS.gene059162:CDS	55.0%
!	ACGACCGTGTTTTTCGTCGG+AGG	+	chr1.2:12165153-12165172	MS.gene059162:CDS	55.0%
	AACTCCACCACCGCGAACGA+CGG	+	chr1.2:12165180-12165199	MS.gene059162:CDS	60.0%
	GATATAGCCGTCGTTCGCGG+TGG	-	chr1.2:12165190-12165209	None:intergenic	60.0%
!!	AGGAAGCACCAGGGAAGCCT+AGG	+	chr1.2:12165227-12165246	MS.gene059162:CDS	60.0%
	ATAGCCGTCGTTCGCGGTGG+TGG	-	chr1.2:12165187-12165206	None:intergenic	65.0%
!!	GTCGTTCGCGGTGGTGGAGT+TGG	-	chr1.2:12165181-12165200	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	12165055	12165621	12165055	ID=MS.gene059162
chr1.2	mRNA	12165055	12165621	12165055	ID=MS.gene059162.t1;Parent=MS.gene059162
chr1.2	exon	12165055	12165621	12165055	ID=MS.gene059162.t1.exon1;Parent=MS.gene059162.t1
chr1.2	CDS	12165055	12165621	12165055	ID=cds.MS.gene059162.t1;Parent=MS.gene059162.t1

Gene Sequence

>MS.gene059162

ATGACTTCAGAGAATAACAAGAAACTTGATCATGATGATGATGAATTAAGGAGCAACTACAACACACCACAAAAACTTTCTCTTCCACCAGTGTTATCACGACCGTGTTTTTCGTCGGAGGCACCAACTCCACCACCGCGAACGACGGCTATATCGATACCGTTTAAGTGGGAGGAAGCACCAGGGAAGCCTAGGTCATGTCATACACGACCAGAGCCAAAAGAAAGAGAAGGTAACAACGTTGTTAGAGCTTTGGAACTTCCTCCTAGGTTGTTGTCTTTGGAGAGGGAGAGTACTAGCAATATTTACGCACCATCACCAACAACTGTTTTGGAAGGTCCATACGTTGGACGTGCAGTGTCTTTTACGACGTCGTATAGGGATAGTAATAACAAGGGCAGTGTGAATTTTGGGTCTAGTAGGTGGGGTGGGTTGAAGAAAAATAATAGAATTGACCGTGAGGGTAGTTTTGACTTTTCAAGTTGGAGTGTTGATGGGGGTGATAAGGTGAAGATCACAAGAGTTAAGAGAAGGGGAAGCTTCTCACATGGTACTTCACATTTGTGG

Protein sequence

>MS.gene059162.t1

MTSENNKKLDHDDDELRSNYNTPQKLSLPPVLSRPCFSSEAPTPPPRTTAISIPFKWEEAPGKPRSCHTRPEPKEREGNNVVRALELPPRLLSLERESTSNIYAPSPTTVLEGPYVGRAVSFTTSYRDSNNKGSVNFGSSRWGGLKKNNRIDREGSFDFSSWSVDGGDKVKITRVKRRGSFSHGTSHLW