Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030951.t1 | XP_003629947.1 | 99.5 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 2.00E-259 | 904.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030951.t1 | Q96254 | 85.8 | 444 | 63 | 0 | 1 | 444 | 1 | 444 | 6.0e-228 | 791.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030951.t1 | G7L8T3 | 99.5 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 1.5e-259 | 904.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene030951 | MS.gene033585 | PPI |
| MS.gene030951 | MS.gene063577 | PPI |
| MS.gene030951 | MS.gene47883 | PPI |
| MS.gene030951 | MS.gene061580 | PPI |
| MS.gene030951 | MS.gene073174 | PPI |
| MS.gene030951 | MS.gene55072 | PPI |
| MS.gene030951 | MS.gene017310 | PPI |
| MS.gene030951 | MS.gene05029 | PPI |
| MS.gene030951 | MS.gene48050 | PPI |
| MS.gene030951 | MS.gene26196 | PPI |
| MS.gene030951 | MS.gene99137 | PPI |
| MS.gene030951 | MS.gene065419 | PPI |
| MS.gene030951 | MS.gene009580 | PPI |
| MS.gene030951 | MS.gene69853 | PPI |
| MS.gene030951 | MS.gene75479 | PPI |
| MS.gene030951 | MS.gene008351 | PPI |
| MS.gene030951 | MS.gene47882 | PPI |
| MS.gene030951 | MS.gene024151 | PPI |
| MS.gene030951 | MS.gene79211 | PPI |
| MS.gene030951 | MS.gene049680 | PPI |
| MS.gene030951 | MS.gene037879 | PPI |
| MS.gene030951 | MS.gene27927 | PPI |
| MS.gene030951 | MS.gene055394 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030951.t1 | MTR_8g088620 | 99.550 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 0.0 | 917 |
| MS.gene030951.t1 | MTR_4g093980 | 81.193 | 436 | 82 | 0 | 1 | 436 | 1 | 436 | 0.0 | 749 |
| MS.gene030951.t1 | MTR_5g024150 | 25.587 | 426 | 235 | 13 | 5 | 356 | 18 | 435 | 1.00e-26 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene030951.t1 | AT2G44100 | 85.811 | 444 | 63 | 0 | 1 | 444 | 1 | 444 | 0.0 | 802 |
| MS.gene030951.t1 | AT3G59920 | 85.360 | 444 | 65 | 0 | 1 | 444 | 1 | 444 | 0.0 | 798 |
| MS.gene030951.t1 | AT2G44100 | 85.814 | 430 | 61 | 0 | 1 | 430 | 1 | 430 | 0.0 | 781 |
| MS.gene030951.t1 | AT5G09550 | 79.505 | 444 | 91 | 0 | 1 | 444 | 1 | 444 | 0.0 | 754 |
| MS.gene030951.t1 | AT3G06540 | 24.661 | 442 | 250 | 15 | 5 | 366 | 15 | 453 | 1.89e-20 | 94.4 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACCCATGACAGAAAATT+TGG | 0.184972 | 4.3:+11821383 | MS.gene030951:intron |
| CACTTCCCTTAATCTTATTC+AGG | 0.214984 | 4.3:+11819978 | MS.gene030951:CDS |
| GATGATCAAGGAAAGGTTAT+TGG | 0.226106 | 4.3:+11823069 | MS.gene030951:CDS |
| GGCTTTGAAGTCCCCACTTA+TGG | 0.233527 | 4.3:+11820768 | MS.gene030951:CDS |
| TTTGGGTGTTTAGTTCATTA+TGG | 0.255862 | 4.3:+11820502 | MS.gene030951:intron |
| GCACGGCTTAGCGCTGTTTA+TGG | 0.259796 | 4.3:+11821850 | MS.gene030951:CDS |
| TTCAGCTCTGGAAGAAATTC+AGG | 0.276301 | 4.3:+11820090 | MS.gene030951:intron |
| TGACAAGCCTCCTCCTCATT+TGG | 0.302661 | 4.3:+11820118 | MS.gene030951:CDS |
| AGTATATCTACCTTGCATTC+AGG | 0.307677 | 4.3:-11821895 | None:intergenic |
| AACATGTATACGTTGATAAC+TGG | 0.312028 | 4.3:+11826050 | MS.gene030951:CDS |
| CCAGGTTTCAGTTCAATTGC+AGG | 0.316392 | 4.3:-11825802 | None:intergenic |
| AGAATACGATGTAATCGTTC+TGG | 0.319703 | 4.3:+11819768 | MS.gene030951:CDS |
| ATATATCCTTTGTATGGTTT+AGG | 0.331062 | 4.3:+11821690 | MS.gene030951:CDS |
| AAACCTGGAATTGACCTTCT+AGG | 0.335092 | 4.3:+11825817 | MS.gene030951:CDS |
| GACAAGCCTCCTCCTCATTT+GGG | 0.351742 | 4.3:+11820119 | MS.gene030951:CDS |
| GGACTTCATTGGTCATGCTT+TGG | 0.364793 | 4.3:+11821424 | MS.gene030951:CDS |
| GATTGGGAGTCATTGGTGTT+GGG | 0.368885 | 4.3:-11823475 | None:intergenic |
| TCTAGGGTGGGTTCATTGAC+TGG | 0.390022 | 4.3:-11825883 | None:intergenic |
| TATTCTCCCACAAAAGCAAC+TGG | 0.403427 | 4.3:+11823507 | MS.gene030951:CDS |
| GCTTTGAAGTCCCCACTTAT+GGG | 0.406357 | 4.3:+11820769 | MS.gene030951:CDS |
| GAGTCTCTTGCACGATTTCA+AGG | 0.410458 | 4.3:+11821654 | MS.gene030951:CDS |
| CCTTACATATATCCTTTGTA+TGG | 0.411629 | 4.3:+11821684 | MS.gene030951:CDS |
| TCTTTACTCTTCTGCAGCAC+TGG | 0.413041 | 4.3:-11826676 | None:intergenic |
| TGACTTACATGTCTGACTTT+CGG | 0.427909 | 4.3:-11823531 | None:intergenic |
| CGATTGGGAGTCATTGGTGT+TGG | 0.428832 | 4.3:-11823476 | None:intergenic |
| ACCTTGTTGGTCAAGTATGA+TGG | 0.428877 | 4.3:-11823141 | None:intergenic |
| GGTCCTAGAAGGTCAATTCC+AGG | 0.437493 | 4.3:-11825820 | None:intergenic |
| CTGAATAAGATTAAGGGAAG+TGG | 0.439368 | 4.3:-11819977 | None:intergenic |
| AAAGCCTCCATGTCATTTGA+AGG | 0.440668 | 4.3:-11820751 | None:intergenic |
| TCTCACAATGTTTGCCCAAA+GGG | 0.442192 | 4.3:+11825736 | MS.gene030951:CDS |
| TTGCACCGATTGGGAGTCAT+TGG | 0.442455 | 4.3:-11823482 | None:intergenic |
| TCGAAATAAAGCAGTTGTCT+AGG | 0.450436 | 4.3:-11825900 | None:intergenic |
| CCTGCAATTGAACTGAAACC+TGG | 0.453263 | 4.3:+11825802 | MS.gene030951:CDS |
| GAGTCATTGGTGTTGGGGAT+CGG | 0.454805 | 4.3:-11823469 | None:intergenic |
| CTCTTCACAGTGTCTAGTGC+TGG | 0.455178 | 4.3:-11821479 | None:intergenic |
| GATAACACTGTGGACTTCAT+TGG | 0.464458 | 4.3:+11821413 | MS.gene030951:CDS |
| GAGAATAACTTGCACCGATT+GGG | 0.465079 | 4.3:-11823491 | None:intergenic |
| GATGTAATCGTTCTGGGAAC+TGG | 0.470059 | 4.3:+11819775 | MS.gene030951:CDS |
| ATGGATAGAAATGATTATTA+CGG | 0.478912 | 4.3:+11819946 | MS.gene030951:CDS |
| TCTCATCAACAGGTCCTAGA+AGG | 0.482856 | 4.3:-11825831 | None:intergenic |
| TTTCAAATATTCCCATAAGT+GGG | 0.483406 | 4.3:-11820780 | None:intergenic |
| GGAGAATAACTTGCACCGAT+TGG | 0.484322 | 4.3:-11823492 | None:intergenic |
| GAATACGATGTAATCGTTCT+GGG | 0.496229 | 4.3:+11819769 | MS.gene030951:CDS |
| CGAAATAAAGCAGTTGTCTA+GGG | 0.502383 | 4.3:-11825899 | None:intergenic |
| CTCTATTTCAAAGCTGTGGA+TGG | 0.502434 | 4.3:+11820575 | MS.gene030951:CDS |
| TCACTTTGAATCAACAGTAC+TGG | 0.502679 | 4.3:+11826019 | MS.gene030951:CDS |
| GTTATTGGCGTCACGTCTGA+AGG | 0.507245 | 4.3:+11823084 | MS.gene030951:CDS |
| GTTTAGTTCATTATGGCCAA+TGG | 0.508001 | 4.3:+11820509 | MS.gene030951:intron |
| CAGGTGGAATTTGATGATCA+AGG | 0.509665 | 4.3:+11823057 | MS.gene030951:intron |
| CCATACAAAGGATATATGTA+AGG | 0.512388 | 4.3:-11821684 | None:intergenic |
| AGTGGTCTTCTCTCCGTCGA+TGG | 0.517094 | 4.3:+11819817 | MS.gene030951:CDS |
| AACCCTCACTAATGTGCCAT+TGG | 0.520084 | 4.3:-11820525 | None:intergenic |
| ACCATTTCAACAGGTGAGGA+AGG | 0.530785 | 4.3:+11823408 | MS.gene030951:intron |
| TTTCAACAGGTGAGGAAGGT+TGG | 0.530894 | 4.3:+11823412 | MS.gene030951:intron |
| GCTGATCCTAAAACTCATGA+TGG | 0.539416 | 4.3:+11820850 | MS.gene030951:CDS |
| CTAGAACCCAAATGAGGAGG+AGG | 0.539538 | 4.3:-11820125 | None:intergenic |
| TCCTAAAACTCATGATGGGA+TGG | 0.544577 | 4.3:+11820855 | MS.gene030951:CDS |
| ACAGGTGAGGAAGGTTGGTA+AGG | 0.545562 | 4.3:+11823417 | MS.gene030951:intron |
| TTCTCACAATGTTTGCCCAA+AGG | 0.554054 | 4.3:+11825735 | MS.gene030951:CDS |
| TGGTTTAGGAGAGCTTCCTC+AGG | 0.555597 | 4.3:+11821704 | MS.gene030951:CDS |
| GTTGAATAAACCTGAATGCA+AGG | 0.558302 | 4.3:+11821885 | MS.gene030951:CDS |
| ATTGGGAGTCATTGGTGTTG+GGG | 0.558324 | 4.3:-11823474 | None:intergenic |
| GAGAATACGAGCAACAGAAT+AGG | 0.558919 | 4.3:-11825717 | None:intergenic |
| TATTGGTACAGGCATTTGCA+CGG | 0.559199 | 4.3:+11821833 | MS.gene030951:intron |
| CATTGGTGTTGGGGATCGGA+TGG | 0.560062 | 4.3:-11823465 | None:intergenic |
| CAAGGTACCTTCAAATGACA+TGG | 0.560179 | 4.3:+11820744 | MS.gene030951:CDS |
| GGAAGGTTGGTAAGGTTGCA+AGG | 0.561162 | 4.3:+11823425 | MS.gene030951:CDS |
| AGTCTCTTGCACGATTTCAA+GGG | 0.561340 | 4.3:+11821655 | MS.gene030951:CDS |
| CGGCTTAGCGCTGTTTATGG+AGG | 0.561599 | 4.3:+11821853 | MS.gene030951:CDS |
| GGAATTTGATGATCAAGGAA+AGG | 0.566365 | 4.3:+11823062 | MS.gene030951:CDS |
| TTCAAATATTCCCATAAGTG+GGG | 0.568438 | 4.3:-11820779 | None:intergenic |
| TTATGTTCAAGATTATAATG+AGG | 0.568766 | 4.3:+11820828 | MS.gene030951:CDS |
| CTTGACAAGAGTCACTACTA+AGG | 0.569477 | 4.3:+11820879 | MS.gene030951:CDS |
| CTTTGTCTTTAACAAAGCAA+AGG | 0.569984 | 4.3:+11820601 | MS.gene030951:CDS |
| TGGCCAATGGCACATTAGTG+AGG | 0.571838 | 4.3:+11820522 | MS.gene030951:CDS |
| AATAAAGCAGTTGTCTAGGG+TGG | 0.573026 | 4.3:-11825896 | None:intergenic |
| TCAGCTCTGGAAGAAATTCA+GGG | 0.573618 | 4.3:+11820091 | MS.gene030951:intron |
| GTATCTCTATTTCAAAGCTG+TGG | 0.574401 | 4.3:+11820571 | MS.gene030951:CDS |
| GTCTCTTGCACGATTTCAAG+GGG | 0.577654 | 4.3:+11821656 | MS.gene030951:CDS |
| CAACACCAATGACTCCCAAT+CGG | 0.583889 | 4.3:+11823477 | MS.gene030951:CDS |
| AGTCCACAGTGTTATCATCA+AGG | 0.587304 | 4.3:-11821406 | None:intergenic |
| GGCCAATGGCACATTAGTGA+GGG | 0.588085 | 4.3:+11820523 | MS.gene030951:CDS |
| GGTTACTTTGAGGCCATCGA+CGG | 0.588114 | 4.3:-11819830 | None:intergenic |
| CTGATCCTAAAACTCATGAT+GGG | 0.593086 | 4.3:+11820851 | MS.gene030951:CDS |
| TAATATTGATATGAACCCTA+AGG | 0.595316 | 4.3:+11820157 | MS.gene030951:CDS |
| TACTGTTGATTCAAAGTGAG+TGG | 0.602582 | 4.3:-11826015 | None:intergenic |
| GAAGGTTGGTAAGGTTGCAA+GGG | 0.604709 | 4.3:+11823426 | MS.gene030951:CDS |
| ATAAAGCAGTTGTCTAGGGT+GGG | 0.607677 | 4.3:-11825895 | None:intergenic |
| TTATTGGCGTCACGTCTGAA+GGG | 0.608673 | 4.3:+11823085 | MS.gene030951:CDS |
| GGGGATCGGATGGCTCATGA+TGG | 0.613814 | 4.3:-11823455 | None:intergenic |
| GTATACGTTGATAACTGGAA+AGG | 0.621367 | 4.3:+11826055 | MS.gene030951:CDS |
| GATAGAAATGATTATTACGG+AGG | 0.624916 | 4.3:+11819949 | MS.gene030951:CDS |
| TTATCAACGTATACATGTTG+AGG | 0.629009 | 4.3:-11826046 | None:intergenic |
| TATTGGCGTCACGTCTGAAG+GGG | 0.631609 | 4.3:+11823086 | MS.gene030951:CDS |
| TTACTCTTCTGCAGCACTGG+CGG | 0.636142 | 4.3:-11826673 | None:intergenic |
| AGACACTGTGAAGAGAATGA+AGG | 0.644888 | 4.3:+11821487 | MS.gene030951:CDS |
| TCTTTGCTAGAACCCAAATG+AGG | 0.649689 | 4.3:-11820131 | None:intergenic |
| TATTGCATTTGTATCAACTG+AGG | 0.650673 | 4.3:+11825765 | MS.gene030951:CDS |
| AGCTCTCCTAAACCATACAA+AGG | 0.658728 | 4.3:-11821696 | None:intergenic |
| TTGCTAGAACCCAAATGAGG+AGG | 0.659828 | 4.3:-11820128 | None:intergenic |
| ATTCTCCCACAAAAGCAACT+GGG | 0.662559 | 4.3:+11823508 | MS.gene030951:CDS |
| TCCATCATACTTGACCAACA+AGG | 0.680703 | 4.3:+11823140 | MS.gene030951:CDS |
| CTCAAAGAATGCATTCTCAG+TGG | 0.682881 | 4.3:+11819799 | MS.gene030951:CDS |
| CAGCTCTGGAAGAAATTCAG+GGG | 0.685860 | 4.3:+11820092 | MS.gene030951:intron |
| GGTACCTTCAAATGACATGG+AGG | 0.738325 | 4.3:+11820747 | MS.gene030951:CDS |
| CGGCACTGAGATCCACAGTG+AGG | 0.743753 | 4.3:-11826653 | None:intergenic |
| TCTCTTGCACGATTTCAAGG+GGG | 0.764721 | 4.3:+11821657 | MS.gene030951:CDS |
| TGGCCTTGATGATAACACTG+TGG | 0.769317 | 4.3:+11821403 | MS.gene030951:CDS |
| ACAGGTTCTAGACCTCACTG+TGG | 0.826301 | 4.3:+11826641 | MS.gene030951:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 11819760 | 11826695 | 11819760 | ID=MS.gene030951 |
| chr4.3 | mRNA | 11819760 | 11826695 | 11819760 | ID=MS.gene030951.t1;Parent=MS.gene030951 |
| chr4.3 | exon | 11819760 | 11819846 | 11819760 | ID=MS.gene030951.t1.exon1;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11819760 | 11819846 | 11819760 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11819937 | 11819999 | 11819937 | ID=MS.gene030951.t1.exon2;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11819937 | 11819999 | 11819937 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11820095 | 11820178 | 11820095 | ID=MS.gene030951.t1.exon3;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11820095 | 11820178 | 11820095 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11820515 | 11820622 | 11820515 | ID=MS.gene030951.t1.exon4;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11820515 | 11820622 | 11820515 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11820739 | 11820911 | 11820739 | ID=MS.gene030951.t1.exon5;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11820739 | 11820911 | 11820739 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11821397 | 11821508 | 11821397 | ID=MS.gene030951.t1.exon6;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11821397 | 11821508 | 11821397 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11821645 | 11821725 | 11821645 | ID=MS.gene030951.t1.exon7;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11821645 | 11821725 | 11821645 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11821844 | 11821906 | 11821844 | ID=MS.gene030951.t1.exon8;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11821844 | 11821906 | 11821844 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11823060 | 11823161 | 11823060 | ID=MS.gene030951.t1.exon9;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11823060 | 11823161 | 11823060 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11823421 | 11823545 | 11823421 | ID=MS.gene030951.t1.exon10;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11823421 | 11823545 | 11823421 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11825714 | 11825924 | 11825714 | ID=MS.gene030951.t1.exon11;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11825714 | 11825924 | 11825714 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11826002 | 11826076 | 11826002 | ID=MS.gene030951.t1.exon12;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11826002 | 11826076 | 11826002 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| chr4.3 | exon | 11826645 | 11826695 | 11826645 | ID=MS.gene030951.t1.exon13;Parent=MS.gene030951.t1 |
| chr4.3 | CDS | 11826645 | 11826695 | 11826645 | ID=cds.MS.gene030951.t1;Parent=MS.gene030951.t1 |
| Gene Sequence |
| Protein sequence |
