Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47883.t1 | XP_003629947.1 | 99.5 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 2.00E-259 | 904.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47883.t1 | Q96254 | 85.8 | 444 | 63 | 0 | 1 | 444 | 1 | 444 | 6.0e-228 | 791.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47883.t1 | G7L8T3 | 99.5 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 1.5e-259 | 904.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049402 | MS.gene47883 | 0.805531 | 1.28E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene47883 | -0.805672 | 1.19E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene47883 | -0.804823 | 1.80E-49 | -1.69E-46 |
| MS.gene050833 | MS.gene47883 | 0.80061 | 1.33E-48 | -1.69E-46 |
| MS.gene051517 | MS.gene47883 | 0.803369 | 3.60E-49 | -1.69E-46 |
| MS.gene053776 | MS.gene47883 | 0.81166 | 6.27E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene47883 | 0.822003 | 2.99E-53 | -1.69E-46 |
| MS.gene055502 | MS.gene47883 | -0.810893 | 9.19E-51 | -1.69E-46 |
| MS.gene057606 | MS.gene47883 | 0.80308 | 4.13E-49 | -1.69E-46 |
| MS.gene058452 | MS.gene47883 | 0.800303 | 1.54E-48 | -1.69E-46 |
| MS.gene059677 | MS.gene47883 | -0.82307 | 1.69E-53 | -1.69E-46 |
| MS.gene060694 | MS.gene47883 | 0.804256 | 2.36E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene47883 | MS.gene065419 | PPI |
| MS.gene033585 | MS.gene47883 | PPI |
| MS.gene27927 | MS.gene47883 | PPI |
| MS.gene063577 | MS.gene47883 | PPI |
| MS.gene55072 | MS.gene47883 | PPI |
| MS.gene030951 | MS.gene47883 | PPI |
| MS.gene037879 | MS.gene47883 | PPI |
| MS.gene47882 | MS.gene47883 | PPI |
| MS.gene99137 | MS.gene47883 | PPI |
| MS.gene073174 | MS.gene47883 | PPI |
| MS.gene049680 | MS.gene47883 | PPI |
| MS.gene79211 | MS.gene47883 | PPI |
| MS.gene055394 | MS.gene47883 | PPI |
| MS.gene75479 | MS.gene47883 | PPI |
| MS.gene05029 | MS.gene47883 | PPI |
| MS.gene47883 | MS.gene061580 | PPI |
| MS.gene009580 | MS.gene47883 | PPI |
| MS.gene008351 | MS.gene47883 | PPI |
| MS.gene47883 | MS.gene017310 | PPI |
| MS.gene47883 | MS.gene48050 | PPI |
| MS.gene47883 | MS.gene26196 | PPI |
| MS.gene024151 | MS.gene47883 | PPI |
| MS.gene69853 | MS.gene47883 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47883.t1 | MTR_8g088620 | 99.550 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 0.0 | 917 |
| MS.gene47883.t1 | MTR_4g093980 | 81.193 | 436 | 82 | 0 | 1 | 436 | 1 | 436 | 0.0 | 749 |
| MS.gene47883.t1 | MTR_5g024150 | 25.587 | 426 | 235 | 13 | 5 | 356 | 18 | 435 | 1.00e-26 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene47883.t1 | AT2G44100 | 85.811 | 444 | 63 | 0 | 1 | 444 | 1 | 444 | 0.0 | 802 |
| MS.gene47883.t1 | AT3G59920 | 85.360 | 444 | 65 | 0 | 1 | 444 | 1 | 444 | 0.0 | 798 |
| MS.gene47883.t1 | AT2G44100 | 85.814 | 430 | 61 | 0 | 1 | 430 | 1 | 430 | 0.0 | 781 |
| MS.gene47883.t1 | AT5G09550 | 79.505 | 444 | 91 | 0 | 1 | 444 | 1 | 444 | 0.0 | 754 |
| MS.gene47883.t1 | AT3G06540 | 24.661 | 442 | 250 | 15 | 5 | 366 | 15 | 453 | 1.89e-20 | 94.4 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACCCATGACAGAAAATT+TGG | 0.184972 | 4.1:+11990382 | MS.gene47883:intron |
| CACTTCCCTTAATCTTATTC+AGG | 0.214984 | 4.1:+11988978 | MS.gene47883:CDS |
| GATGATCAAGGAAAGGTTAT+TGG | 0.226106 | 4.1:+11992068 | MS.gene47883:CDS |
| GGCTTTGAAGTCCCCACTTA+TGG | 0.233527 | 4.1:+11989767 | MS.gene47883:CDS |
| TTTGGGTGTTTAGTTCATTA+TGG | 0.255862 | 4.1:+11989501 | MS.gene47883:intron |
| GCACGGCTTAGCGCTGTTTA+TGG | 0.259796 | 4.1:+11990849 | MS.gene47883:CDS |
| TTCAGCTCTGGAAGAAATTC+AGG | 0.276301 | 4.1:+11989089 | MS.gene47883:intron |
| TGACAAGCCTCCTCCTCATT+TGG | 0.302661 | 4.1:+11989117 | MS.gene47883:CDS |
| AGTATATCTACCTTGCATTC+AGG | 0.307677 | 4.1:-11990894 | None:intergenic |
| AACATGTATACGTTGATAAC+TGG | 0.312028 | 4.1:+11995060 | MS.gene47883:CDS |
| CCAGGTTTCAGTTCAATTGC+AGG | 0.316392 | 4.1:-11994812 | None:intergenic |
| AGAATACGATGTAATCGTTC+TGG | 0.319703 | 4.1:+11988768 | MS.gene47883:CDS |
| ATATATCCTTTGTATGGTTT+AGG | 0.331062 | 4.1:+11990689 | MS.gene47883:CDS |
| AAACCTGGAATTGACCTTCT+AGG | 0.335092 | 4.1:+11994827 | MS.gene47883:CDS |
| GACAAGCCTCCTCCTCATTT+GGG | 0.351742 | 4.1:+11989118 | MS.gene47883:CDS |
| GGACTTCATTGGTCATGCTT+TGG | 0.364793 | 4.1:+11990423 | MS.gene47883:CDS |
| GATTGGGAGTCATTGGTGTT+GGG | 0.368885 | 4.1:-11992474 | None:intergenic |
| TCTAGGGTGGGTTCATTGAC+TGG | 0.390022 | 4.1:-11994893 | None:intergenic |
| TATTCTCCCACAAAAGCAAC+TGG | 0.403427 | 4.1:+11992506 | MS.gene47883:CDS |
| GCTTTGAAGTCCCCACTTAT+GGG | 0.406357 | 4.1:+11989768 | MS.gene47883:CDS |
| GAGTCTCTTGCACGATTTCA+AGG | 0.410458 | 4.1:+11990653 | MS.gene47883:CDS |
| CCTTACATATATCCTTTGTA+TGG | 0.411629 | 4.1:+11990683 | MS.gene47883:CDS |
| TCTTTACTCTTCTGCAGCAC+TGG | 0.413041 | 4.1:-11995686 | None:intergenic |
| TGACTTACATGTCTGACTTT+CGG | 0.427909 | 4.1:-11992530 | None:intergenic |
| CGATTGGGAGTCATTGGTGT+TGG | 0.428832 | 4.1:-11992475 | None:intergenic |
| ACCTTGTTGGTCAAGTATGA+TGG | 0.428877 | 4.1:-11992140 | None:intergenic |
| GGTCCTAGAAGGTCAATTCC+AGG | 0.437493 | 4.1:-11994830 | None:intergenic |
| CTGAATAAGATTAAGGGAAG+TGG | 0.439368 | 4.1:-11988977 | None:intergenic |
| AAAGCCTCCATGTCATTTGA+AGG | 0.440668 | 4.1:-11989750 | None:intergenic |
| TCTCACAATGTTTGCCCAAA+GGG | 0.442192 | 4.1:+11994746 | MS.gene47883:CDS |
| TTGCACCGATTGGGAGTCAT+TGG | 0.442455 | 4.1:-11992481 | None:intergenic |
| TCGAAATAAAGCAGTTGTCT+AGG | 0.450436 | 4.1:-11994910 | None:intergenic |
| CCTGCAATTGAACTGAAACC+TGG | 0.453263 | 4.1:+11994812 | MS.gene47883:CDS |
| GAGTCATTGGTGTTGGGGAT+CGG | 0.454805 | 4.1:-11992468 | None:intergenic |
| CTCTTCACAGTGTCTAGTGC+TGG | 0.455178 | 4.1:-11990478 | None:intergenic |
| GATAACACTGTGGACTTCAT+TGG | 0.464458 | 4.1:+11990412 | MS.gene47883:CDS |
| GAGAATAACTTGCACCGATT+GGG | 0.465079 | 4.1:-11992490 | None:intergenic |
| GATGTAATCGTTCTGGGAAC+TGG | 0.470059 | 4.1:+11988775 | MS.gene47883:CDS |
| ATGGATAGAAATGATTATTA+CGG | 0.478912 | 4.1:+11988946 | MS.gene47883:CDS |
| TCTCATCAACAGGTCCTAGA+AGG | 0.482856 | 4.1:-11994841 | None:intergenic |
| TTTCAAATATTCCCATAAGT+GGG | 0.483406 | 4.1:-11989779 | None:intergenic |
| GGAGAATAACTTGCACCGAT+TGG | 0.484322 | 4.1:-11992491 | None:intergenic |
| GAATACGATGTAATCGTTCT+GGG | 0.496229 | 4.1:+11988769 | MS.gene47883:CDS |
| CGAAATAAAGCAGTTGTCTA+GGG | 0.502383 | 4.1:-11994909 | None:intergenic |
| CTCTATTTCAAAGCTGTGGA+TGG | 0.502434 | 4.1:+11989574 | MS.gene47883:CDS |
| TCACTTTGAATCAACAGTAC+TGG | 0.502679 | 4.1:+11995029 | MS.gene47883:CDS |
| GTTATTGGCGTCACGTCTGA+AGG | 0.507245 | 4.1:+11992083 | MS.gene47883:CDS |
| GTTTAGTTCATTATGGCCAA+TGG | 0.508001 | 4.1:+11989508 | MS.gene47883:intron |
| CAGGTGGAATTTGATGATCA+AGG | 0.509665 | 4.1:+11992056 | MS.gene47883:intron |
| CCATACAAAGGATATATGTA+AGG | 0.512388 | 4.1:-11990683 | None:intergenic |
| AGTGGTCTTCTCTCCGTCGA+TGG | 0.517094 | 4.1:+11988817 | MS.gene47883:CDS |
| AACCCTCACTAATGTGCCAT+TGG | 0.520084 | 4.1:-11989524 | None:intergenic |
| ACCATTTCAACAGGTGAGGA+AGG | 0.530785 | 4.1:+11992407 | MS.gene47883:intron |
| TTTCAACAGGTGAGGAAGGT+TGG | 0.530894 | 4.1:+11992411 | MS.gene47883:intron |
| GCTGATCCTAAAACTCATGA+TGG | 0.539416 | 4.1:+11989849 | MS.gene47883:CDS |
| CTAGAACCCAAATGAGGAGG+AGG | 0.539538 | 4.1:-11989124 | None:intergenic |
| TCCTAAAACTCATGATGGGA+TGG | 0.544577 | 4.1:+11989854 | MS.gene47883:CDS |
| ACAGGTGAGGAAGGTTGGTA+AGG | 0.545562 | 4.1:+11992416 | MS.gene47883:intron |
| TTCTCACAATGTTTGCCCAA+AGG | 0.554054 | 4.1:+11994745 | MS.gene47883:CDS |
| TGGTTTAGGAGAGCTTCCTC+AGG | 0.555597 | 4.1:+11990703 | MS.gene47883:CDS |
| GTTGAATAAACCTGAATGCA+AGG | 0.558302 | 4.1:+11990884 | MS.gene47883:CDS |
| ATTGGGAGTCATTGGTGTTG+GGG | 0.558324 | 4.1:-11992473 | None:intergenic |
| GAGAATACGAGCAACAGAAT+AGG | 0.558919 | 4.1:-11994727 | None:intergenic |
| TATTGGTACAGGCATTTGCA+CGG | 0.559199 | 4.1:+11990832 | MS.gene47883:intron |
| CATTGGTGTTGGGGATCGGA+TGG | 0.560062 | 4.1:-11992464 | None:intergenic |
| CAAGGTACCTTCAAATGACA+TGG | 0.560179 | 4.1:+11989743 | MS.gene47883:CDS |
| GGAAGGTTGGTAAGGTTGCA+AGG | 0.561162 | 4.1:+11992424 | MS.gene47883:CDS |
| AGTCTCTTGCACGATTTCAA+GGG | 0.561340 | 4.1:+11990654 | MS.gene47883:CDS |
| CGGCTTAGCGCTGTTTATGG+AGG | 0.561599 | 4.1:+11990852 | MS.gene47883:CDS |
| GGAATTTGATGATCAAGGAA+AGG | 0.566365 | 4.1:+11992061 | MS.gene47883:CDS |
| TTCAAATATTCCCATAAGTG+GGG | 0.568438 | 4.1:-11989778 | None:intergenic |
| TTATGTTCAAGATTATAATG+AGG | 0.568766 | 4.1:+11989827 | MS.gene47883:CDS |
| CTTGACAAGAGTCACTACTA+AGG | 0.569477 | 4.1:+11989878 | MS.gene47883:CDS |
| CTTTGTCTTTAACAAAGCAA+AGG | 0.569984 | 4.1:+11989600 | MS.gene47883:CDS |
| TGGCCAATGGCACATTAGTG+AGG | 0.571838 | 4.1:+11989521 | MS.gene47883:CDS |
| AATAAAGCAGTTGTCTAGGG+TGG | 0.573026 | 4.1:-11994906 | None:intergenic |
| TCAGCTCTGGAAGAAATTCA+GGG | 0.573618 | 4.1:+11989090 | MS.gene47883:intron |
| GTATCTCTATTTCAAAGCTG+TGG | 0.574401 | 4.1:+11989570 | MS.gene47883:CDS |
| GTCTCTTGCACGATTTCAAG+GGG | 0.577654 | 4.1:+11990655 | MS.gene47883:CDS |
| CAACACCAATGACTCCCAAT+CGG | 0.583889 | 4.1:+11992476 | MS.gene47883:CDS |
| AGTCCACAGTGTTATCATCA+AGG | 0.587304 | 4.1:-11990405 | None:intergenic |
| GGCCAATGGCACATTAGTGA+GGG | 0.588085 | 4.1:+11989522 | MS.gene47883:CDS |
| GGTTACTTTGAGGCCATCGA+CGG | 0.588114 | 4.1:-11988830 | None:intergenic |
| CTGATCCTAAAACTCATGAT+GGG | 0.593086 | 4.1:+11989850 | MS.gene47883:CDS |
| TAATATTGATATGAACCCTA+AGG | 0.595316 | 4.1:+11989156 | MS.gene47883:CDS |
| TACTGTTGATTCAAAGTGAG+TGG | 0.602582 | 4.1:-11995025 | None:intergenic |
| GAAGGTTGGTAAGGTTGCAA+GGG | 0.604709 | 4.1:+11992425 | MS.gene47883:CDS |
| ATAAAGCAGTTGTCTAGGGT+GGG | 0.607677 | 4.1:-11994905 | None:intergenic |
| TTATTGGCGTCACGTCTGAA+GGG | 0.608673 | 4.1:+11992084 | MS.gene47883:CDS |
| GGGGATCGGATGGCTCATGA+TGG | 0.613814 | 4.1:-11992454 | None:intergenic |
| GTATACGTTGATAACTGGAA+AGG | 0.621367 | 4.1:+11995065 | MS.gene47883:CDS |
| GATAGAAATGATTATTACGG+AGG | 0.624916 | 4.1:+11988949 | MS.gene47883:CDS |
| TTATCAACGTATACATGTTG+AGG | 0.629009 | 4.1:-11995056 | None:intergenic |
| TATTGGCGTCACGTCTGAAG+GGG | 0.631609 | 4.1:+11992085 | MS.gene47883:CDS |
| TTACTCTTCTGCAGCACTGG+CGG | 0.636142 | 4.1:-11995683 | None:intergenic |
| AGACACTGTGAAGAGAATGA+AGG | 0.644888 | 4.1:+11990486 | MS.gene47883:CDS |
| TCTTTGCTAGAACCCAAATG+AGG | 0.649689 | 4.1:-11989130 | None:intergenic |
| TATTGCATTTGTATCAACTG+AGG | 0.650673 | 4.1:+11994775 | MS.gene47883:CDS |
| AGCTCTCCTAAACCATACAA+AGG | 0.658728 | 4.1:-11990695 | None:intergenic |
| TTGCTAGAACCCAAATGAGG+AGG | 0.659828 | 4.1:-11989127 | None:intergenic |
| ATTCTCCCACAAAAGCAACT+GGG | 0.662559 | 4.1:+11992507 | MS.gene47883:CDS |
| TCCATCATACTTGACCAACA+AGG | 0.680703 | 4.1:+11992139 | MS.gene47883:CDS |
| CTCAAAGAATGCATTCTCAG+TGG | 0.682881 | 4.1:+11988799 | MS.gene47883:CDS |
| CAGCTCTGGAAGAAATTCAG+GGG | 0.685860 | 4.1:+11989091 | MS.gene47883:intron |
| GGTACCTTCAAATGACATGG+AGG | 0.738325 | 4.1:+11989746 | MS.gene47883:CDS |
| CGGCACTGAGATCCACAGTG+AGG | 0.743753 | 4.1:-11995663 | None:intergenic |
| TCTCTTGCACGATTTCAAGG+GGG | 0.764721 | 4.1:+11990656 | MS.gene47883:CDS |
| TGGCCTTGATGATAACACTG+TGG | 0.769317 | 4.1:+11990402 | MS.gene47883:CDS |
| ACAGGTTCTAGACCTCACTG+TGG | 0.826301 | 4.1:+11995651 | MS.gene47883:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 11988760 | 11995705 | 11988760 | ID=MS.gene47883 |
| chr4.1 | mRNA | 11988760 | 11995705 | 11988760 | ID=MS.gene47883.t1;Parent=MS.gene47883 |
| chr4.1 | exon | 11988760 | 11988846 | 11988760 | ID=MS.gene47883.t1.exon1;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11988760 | 11988846 | 11988760 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11988937 | 11988999 | 11988937 | ID=MS.gene47883.t1.exon2;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11988937 | 11988999 | 11988937 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11989094 | 11989177 | 11989094 | ID=MS.gene47883.t1.exon3;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11989094 | 11989177 | 11989094 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11989514 | 11989621 | 11989514 | ID=MS.gene47883.t1.exon4;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11989514 | 11989621 | 11989514 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11989738 | 11989910 | 11989738 | ID=MS.gene47883.t1.exon5;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11989738 | 11989910 | 11989738 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11990396 | 11990507 | 11990396 | ID=MS.gene47883.t1.exon6;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11990396 | 11990507 | 11990396 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11990644 | 11990724 | 11990644 | ID=MS.gene47883.t1.exon7;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11990644 | 11990724 | 11990644 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11990843 | 11990905 | 11990843 | ID=MS.gene47883.t1.exon8;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11990843 | 11990905 | 11990843 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11992059 | 11992160 | 11992059 | ID=MS.gene47883.t1.exon9;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11992059 | 11992160 | 11992059 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11992420 | 11992544 | 11992420 | ID=MS.gene47883.t1.exon10;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11992420 | 11992544 | 11992420 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11994724 | 11994934 | 11994724 | ID=MS.gene47883.t1.exon11;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11994724 | 11994934 | 11994724 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11995012 | 11995086 | 11995012 | ID=MS.gene47883.t1.exon12;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11995012 | 11995086 | 11995012 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| chr4.1 | exon | 11995655 | 11995705 | 11995655 | ID=MS.gene47883.t1.exon13;Parent=MS.gene47883.t1 |
| chr4.1 | CDS | 11995655 | 11995705 | 11995655 | ID=cds.MS.gene47883.t1;Parent=MS.gene47883.t1 |
| Gene Sequence |
| Protein sequence |
