Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073174.t1 | XP_003596706.1 | 100 | 218 | 0 | 0 | 1 | 218 | 1 | 218 | 1.00E-115 | 426 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073174.t1 | Q40194 | 94.0 | 217 | 13 | 0 | 1 | 217 | 1 | 217 | 4.6e-112 | 405.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073174.t1 | G7IM71 | 100.0 | 218 | 0 | 0 | 1 | 218 | 1 | 218 | 7.5e-116 | 426.0 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene073174 | 0.844351 | 7.98E-59 | -1.69E-46 |
| MS.gene050359 | MS.gene073174 | 0.805424 | 1.34E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene073174 | 0.805856 | 1.09E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene073174 | 0.815827 | 7.57E-52 | -1.69E-46 |
| MS.gene050945 | MS.gene073174 | 0.87035 | 1.56E-66 | -1.69E-46 |
| MS.gene050955 | MS.gene073174 | 0.801059 | 1.08E-48 | -1.69E-46 |
| MS.gene051003 | MS.gene073174 | 0.814167 | 1.77E-51 | -1.69E-46 |
| MS.gene051234 | MS.gene073174 | 0.831453 | 1.65E-55 | -1.69E-46 |
| MS.gene051343 | MS.gene073174 | 0.848225 | 7.02E-60 | -1.69E-46 |
| MS.gene051699 | MS.gene073174 | 0.83291 | 7.22E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene000342 | MS.gene073174 | PPI |
| MS.gene000694 | MS.gene073174 | PPI |
| MS.gene001096 | MS.gene073174 | PPI |
| MS.gene003158 | MS.gene073174 | PPI |
| MS.gene004991 | MS.gene073174 | PPI |
| MS.gene006652 | MS.gene073174 | PPI |
| MS.gene007373 | MS.gene073174 | PPI |
| MS.gene007571 | MS.gene073174 | PPI |
| MS.gene011573 | MS.gene073174 | PPI |
| MS.gene013307 | MS.gene073174 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073174.t1 | MTR_2g083860 | 100.000 | 218 | 0 | 0 | 1 | 218 | 1 | 218 | 2.41e-162 | 447 |
| MS.gene073174.t1 | MTR_2g075950 | 83.871 | 217 | 34 | 1 | 1 | 217 | 1 | 216 | 2.19e-135 | 379 |
| MS.gene073174.t1 | MTR_4g088090 | 76.712 | 219 | 46 | 3 | 1 | 217 | 1 | 216 | 9.68e-122 | 344 |
| MS.gene073174.t1 | MTR_2g014990 | 76.256 | 219 | 47 | 3 | 1 | 217 | 1 | 216 | 2.74e-121 | 343 |
| MS.gene073174.t1 | MTR_4g079350 | 73.973 | 219 | 52 | 3 | 1 | 217 | 1 | 216 | 4.39e-120 | 340 |
| MS.gene073174.t1 | MTR_2g090865 | 73.585 | 212 | 52 | 3 | 7 | 217 | 5 | 213 | 2.12e-112 | 320 |
| MS.gene073174.t1 | MTR_3g069510 | 68.981 | 216 | 66 | 1 | 3 | 218 | 2 | 216 | 1.26e-110 | 316 |
| MS.gene073174.t1 | MTR_4g064897 | 70.093 | 214 | 62 | 2 | 4 | 217 | 3 | 214 | 7.77e-110 | 314 |
| MS.gene073174.t1 | MTR_2g005510 | 66.038 | 212 | 72 | 0 | 7 | 218 | 9 | 220 | 4.52e-102 | 295 |
| MS.gene073174.t1 | MTR_1g052475 | 60.748 | 214 | 80 | 1 | 7 | 216 | 9 | 222 | 2.41e-95 | 278 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073174.t1 | AT1G06400 | 83.486 | 218 | 34 | 1 | 1 | 218 | 1 | 216 | 2.07e-136 | 381 |
| MS.gene073174.t1 | AT1G16920 | 82.569 | 218 | 36 | 2 | 1 | 218 | 1 | 216 | 3.47e-133 | 373 |
| MS.gene073174.t1 | AT4G18800 | 82.569 | 218 | 34 | 2 | 1 | 218 | 1 | 214 | 1.44e-131 | 369 |
| MS.gene073174.t1 | AT5G45750 | 81.651 | 218 | 38 | 1 | 1 | 218 | 1 | 216 | 3.79e-131 | 368 |
| MS.gene073174.t1 | AT5G60860 | 77.273 | 220 | 45 | 3 | 1 | 218 | 1 | 217 | 7.08e-125 | 352 |
| MS.gene073174.t1 | AT3G15060 | 75.455 | 220 | 49 | 3 | 1 | 218 | 1 | 217 | 8.05e-122 | 344 |
| MS.gene073174.t1 | AT4G18430 | 73.059 | 219 | 54 | 3 | 1 | 217 | 1 | 216 | 3.20e-119 | 338 |
| MS.gene073174.t1 | AT1G28550 | 72.146 | 219 | 57 | 3 | 1 | 217 | 1 | 217 | 4.74e-117 | 332 |
| MS.gene073174.t1 | AT2G33870 | 71.233 | 219 | 59 | 3 | 1 | 217 | 1 | 217 | 1.03e-114 | 327 |
| MS.gene073174.t1 | AT1G07410 | 70.423 | 213 | 60 | 2 | 5 | 217 | 4 | 213 | 2.35e-109 | 313 |
Find 52 sgRNAs with CRISPR-Local
Find 423 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGTGAAAGAGGATTCTT+CGG | 0.420021 | 2.3:-14077037 | MS.gene073174:CDS |
| AAAGACTTTCCATCTTCTGT+TGG | 0.448264 | 2.3:+14077231 | None:intergenic |
| AAAGCAGTTGAAGCGGGTGA+AGG | 0.591230 | 2.3:-14077099 | MS.gene073174:CDS |
| AAGACTTTCCATCTTCTGTT+GGG | 0.447210 | 2.3:+14077232 | None:intergenic |
| AAGTCATTAAGGCTCAGATT+TGG | 0.391753 | 2.3:-14082514 | MS.gene073174:CDS |
| AATATTGTGGTCATGCTTAT+TGG | 0.176164 | 2.3:-14077288 | MS.gene073174:CDS |
| ACAGACAATAAACGTGAAAG+AGG | 0.555146 | 2.3:-14077046 | MS.gene073174:CDS |
| AGCAAGAAAGCAGTTGAAGC+GGG | 0.518997 | 2.3:-14077105 | MS.gene073174:CDS |
| AGCATCAACAGTCAAACTCT+TGG | 0.503401 | 2.3:+14082538 | None:intergenic |
| AGCGAATTCAACACCAATAG+TGG | 0.433002 | 2.3:+14082562 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCAAGTCAACAAATGTA+CGG | + | chr2.3:14078103-14078122 | None:intergenic | 30.0% | |
| AACTCAATAACAGACCAATA+TGG | + | chr2.3:14079904-14079923 | None:intergenic | 30.0% | |
| AAGAACTGAACTTGTATCAA+AGG | - | chr2.3:14081067-14081086 | MS.gene073174:intron | 30.0% | |
| AAGAGAAGTAAGATTTGCAA+AGG | + | chr2.3:14078422-14078441 | None:intergenic | 30.0% | |
| AATACGACATAGTGCTTATA+TGG | - | chr2.3:14079463-14079482 | MS.gene073174:intron | 30.0% | |
| AATATTGTGGTCATGCTTAT+TGG | - | chr2.3:14082409-14082428 | MS.gene073174:intron | 30.0% | |
| ACATTTAGTAAATAGAGCAG+TGG | + | chr2.3:14081819-14081838 | None:intergenic | 30.0% | |
| ACCAGAACTTCTAGTAATAT+GGG | + | chr2.3:14080957-14080976 | None:intergenic | 30.0% | |
| ACCTCTATCCTTTATATATG+TGG | + | chr2.3:14079745-14079764 | None:intergenic | 30.0% | |
| AGAGAAGTAAGATTTGCAAA+GGG | + | chr2.3:14078421-14078440 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 14077005 | 14082714 | 14077005 | ID=MS.gene073174 |
| chr2.3 | mRNA | 14077005 | 14082714 | 14077005 | ID=MS.gene073174.t1;Parent=MS.gene073174 |
| chr2.3 | exon | 14082494 | 14082714 | 14082494 | ID=MS.gene073174.t1.exon1;Parent=MS.gene073174.t1 |
| chr2.3 | CDS | 14082494 | 14082714 | 14082494 | ID=cds.MS.gene073174.t1;Parent=MS.gene073174.t1 |
| chr2.3 | exon | 14077005 | 14077440 | 14077005 | ID=MS.gene073174.t1.exon2;Parent=MS.gene073174.t1 |
| chr2.3 | CDS | 14077005 | 14077440 | 14077005 | ID=cds.MS.gene073174.t1;Parent=MS.gene073174.t1 |
| Gene Sequence |
>MS.gene073174 ATGGCTGGATACAGAGCAGACGATGAATACGATTACCTTTTCAAACTAGTTCTAATCGGTGATTCAGGTGTTGGTAAATCCAACTTGCTTTCTAGATTCACTAGAAACGAGTTCAATTTGGAATCTAAATCCACTATTGGTGTTGAATTCGCTACCAAGAGTTTGACTGTTGATGCTAAAGTCATTAAGGCTCAGATTTGGGATACTGCTGGTCAGGAAAGGTCTCTTTTTTTCTCAACCTTTTCTTTAATTTCAATTTTTTATTTATTTGAATTTTGGTTCTGATTGTTTGTTAGAACCCTTATTGATTTGTTCTTATTTCTCAATTGATTTTTTTTTTTTTTTATTTTGATAGAAAAAATGTTAGTAAGTTAGTTGATATGTAGTAGTAGTTTTTAGGGTTCGGATTTTAGACCAAGCTCCTTTCGTATCCCGATTGATTGTTTAAATTTTGAGTTTAATTTTGATACGCTGTTAAAAGGTCGCAATAATTGGACTTTAGAAGTAATTATGATTGAAGTCAAACATTTTTGTTTATTTGCCGGTAATGAGTGATTGACAACAGTATGATTTTGTTTTTACACGGACAGTGTAGAATTCAGATTTCCTGCAGTAGTATGTGAATCACGTATTCAATCATTTTGGACATCGGTGGGTGTCCGATTAGGTCCAAAAAGTGCAGATTTAAATTTAAATTTTACTCCCAATCTTTATCGAACGACTTCCGATGTCTTGAATTCGTGAATGCGAGAAATCCTGACTGCAGGGAAGCCGGATCCGACAGTGTATATGAATTAAATCCTTAAATTTATTGATCACATTATATAGTATTATTTCAAAATCGTTCTTATTGGTTTACGAACTTCAAAGTTTTCTATAAACTGGCATTGGGGATAAGATTTTGCAAAATTATGATCAAGATGCGTGCACATGATAATTTATTGGTGTTTAAATCACTTTCAAGTGGTGTTGTCAAATAGTGGCTATAGTGGCACTATGGCGGTATGGTTTAGGGAATTTGAAAAAATTTCCACCGAGGAATGTCAGCTTTACTCACTTTCCTTTATTACCCTTAATCCTTAAAATCCACAGACGCCGTACATTTGTTGACTTGCTTTATGACCATGGTCCATGTACATACGTGACTTGTTATATAGAGTATTGTGTTGATGACACTGGTGGTCTTTGATCTCAAGATCACGTTTGAGTTATCTGTAAAGCTTCATGTGCTTTATTTTTGTACCTTTTTTTTTTATTTATTTTTATGTTTGAGCCTAACCATCACCAACCTCCTGAAATGATTAGAAAACTATTGGAAAATCATGATTGTTAAAAAATTAGTAATTCTATTTCTTAATAAGTTGTTAATCATGTACAAGTTACAACAACCCAACTGGCCCTGAACATATTTCACCCTTTGCAAATCTTACTTCTCTTTATTAATCCATGTAACCATGGTAGTCAAAATCGTGTGTTTGATCGACGATTGAATGTTTATACTTACCTCAGCGATTAAGAGTACACACACAAGAGAAATACTTGCCTGGGCACAAGCATAGATAAAAACATTCGGGCACACACACAAAACTAAAAAAAATAAAATAAAAGAGAATCAAATTAAATGATGTCAATTGATGTGACTAAATTATTTTTGTTTAATTTTGTCCATTTCATGTGAGTGTGCCCAAATAATATTGATTTGGGGTTGTGCCCATGGAAGACTTGCTCCACACACAATCGGGTTTTGGTGAAATCGGGTGGAATCAATCCCACTGGTTAATTGCAGATTCGTTATTCGTATGATCTGCGATTTCTGGGTGGGAGTTGCGACAACGGCCGCACACGCCAGGCTTACATAAACCCCTTTTCTGGTACGCGGGTTGAGAAAACGACCCATTTCCTATTAGTGGATCATAAAACCCAGTTATACTTATGTTTGTGGGTCTATCTGAAAAAATAGGATTTATAGGGGTTAAATACACGTGCCTCTTCATTTTTTCTTACAAAAACCTCTATTTTTATTTTTAAACACAAGTTACTCATGGGCGGCCTTTTCTCTGCTTCTACCTCATGGCCTTTCTCTGCCTGTGCCTCACATGTATCTCGTGCGTGTCACTCTCTATTTCTGTTCTGTAGGATTAGATGTTCATGGTGTTTTTTTACTAGTTAGTTATTTTGTTTGAGACTTATGAAGTTGAGTTCTAATTTTGTATGACTTTTTCTATTTAATTACTTATGCTTTTAAATTTTAATTTATTACTTGAAGTGTGATTTAAAGTTTTCTTTAGCTTGTATACATTATATGCACATATATGAATTTTTATGAATATATGAGTTTTATGAATTTTGTATAATCATACGATTCGTGTTCCGATTTTACCTCCCCTTTCTGATTCTGCATAAAATCTCAGTTTTAACTACCTTGCGTGTAACCTTGGTGAACTCAGGCCTAATAGTTAATACGACATAGTGCTTATATGGTTGGCGTGCTGCTGGTTACTTGTTTATTGTTTATTCTTCTACACTAGATATCTTCATTTCCCTTCTTTGTAAAACCAACACTTATGATCAAAGACGTGCATGGTGCCCGACATGTACCGATGTCTGACAACATGACACTGTCACATATGGTTACATTCAATTAATTCATTTTTTTTTTTAAATTATTACCGATATCAATGTGTTACTGTCAATGTCATGTCCAATGTTCATGTGTTTTTAAGTGCTTCATGGCGTTCCACATATATAAAGGATAGAGGTAGGTATGGAGCTGCTAAACCTAGTTGCAATGAATTGCAGTAATTAATAAGAGTATTTATAATTTGAATTCCCCCTCCTGTTCTTCTGGTGATTATCACTTAAAAAATAGAAATCATAGAAAAAGATAGTTTCCCAGCCATATTGGTCTGTTATTGAGTTGCTTTAGATTAGGAATATGAAATTTTTATTGAAGAACCCTAGTTCCAATCCTGTCCCAATGCGGATGGGTGCAACACCGGGTGTAGGAAAATGTACAGTAGAATGTAATAATAGGCAGTTAGACTGCTTTTTATGTATTAGTAATATAGTTCCCCCACCCCAAAATGCTTCAAAGGCCATTGACCTTTTCATTCATTCCCCTTACTCCCTCCGTCCCGCAATTGATGACCCATTTGAAAATGTGTAATCTTGACCTAACTTACTTTGACCATATTTTTCTACTAATATACAAAGATAAATAATATCATATAAGATGTCGTTAGATTTGTCTCGATGAGTATTTTCAAAATATCAAATTTTCATAATTTTTTCTATTATATTATTTAAGCTCAAAATTGTGCATTGGCATGCGTAATAAGGTCAACTGTGTCAAGTATTATGAGACGGAGGGAGCACCTTTTTCCCTTTATTTATCAATTATCAATGCCCCCCCCCCCCAAAAAAAAAAAAGAGCTTCCTTGTCCTCAAGGTGGAAAGAAAGGCGCCTTTAACACTAGTAGCCGTATCCCACTTGTTTTCACAATCAAGGAGGTTCAACCAGCTGGCCAATAATTCTTAATTACATGTTCAGTTCTGTCTAGTACTGAGATGTGTTCTGGGCTTTGGCTGGACCTGCAACTTGTATTCATTTGATAGCATGGGGGGAAGTGCTGCTTAGCTGCCACTACTTTCTTCAGCAAGGAGACAAGAAAGACGAGTGAACTTTGCCATGTTAATGCAATTCCGATCTTGATGCAACTTTGTCAATTCTTTCCAACACCTTGTAAGGTCTGTAAAATTGTGGACTGAGTTTCTCATTGTGGTCTATAAATTATATGACTACAATTTTGAAAGACCCATACCCAATCTGTGACTCCAATTCCCTTTGATATTTGTTAGCTCATGCTCATTGCTGATCACGAGCTTACAAATTGTTTCTCAGGTTCTGCCAGCAACTAATCTCTTTCTTCCTGTCTTGCCCCAAGGTTCTCCCATATTACTAGAAGTTCTGGTGTTTACAGTCCTAGCTGCAATTTGTATTATGGCTTTTCTCACCTTTTAGATTTCTATCTCGTTTTTAGCATGCATGTACATACAACCATCAAGAACTGAACTTGTATCAAAGGAATTTATGTCATAAGTCATAACATCATATTCACTTCTGCGGACAACTGCACAATTTGCTCTGAATATACATTTGCATTTTCCAAACCTTATCCAGTTATCCTTATTAATCTATGTAAAACTGATGAACTTATGTTTATTACATAGGTGACAGTGTTGTAAAATAGTGGCGCCATAGCTCTATGGCGTATTGGGATTTTAACAAACTGCTATTGTTTCGCGATACGCGACTTAGTACAAGGTGTTGTCAAATAGCAGCTATAGCACACTATAGCATAGCAGAGTTTTAACAAACCACCATTTTCTGCGATTCACGTTTGACAAAATTACTAGGTGGTCTGTAAGTTGTTCATATGGCAAAGATAGTAGGATGTAGGATGTAGGATGCAGCTGTTACTTGTTTCATATTATTTTGCATATACCCGTAATTTTATCCAAAAAATCTTACCTCCCTGATACACTAACTAGATATATTATGTGACTATGTTAGGATATAGAAGCAGTCATCTATTTGGGACAGTTAATTAGATGGTAGGGAATGATTTGTATAAATAGGAGGAAATCAGAATAGAGATAGCTACTCTCTACATTTGAAAAGAAACATTGAAAGAATGAGAAAGAACAGGGGAGAGAGACAGAGAGAATAGGACAGAGACTCTCATCCAAGTTTTTTATTTTTTTTTGAGGGAGAGATCTCTCATCCAAGTATTTCCCCTACTACAACAGAGCCACTGCTCTATTTACTAAATGTTTACTATACTCAATGGACCCCCTCTATCCTTGCATCCTCATATTTATACAAGATAATGCTTTAACTAACCAACTGCTGTTACTACTCCAATCCCCTTTATTCTATATCCAAACAGGCTATTACTTAAAGATATAGAATGAAGGAAAGTCAAAAGATAGTTATTGCTAGCTTGCCACATGTATCTGATGATGATACTCTATCACATCTACTCAATATATTCTGCGGTAGTGGTTCTAGTTTTGAAGCAGCCTGGTCTCTCTTGTTAGTTATTAGAATTCCTCGATTTTTTTTGCAATTGATTTTTTTTGAATAAACTGAGTTGGTCCGTGCATTTTTGTTGCAAAGCACTATAAAGAAATTAGTTATGCCGTTAACAATGGGCGGAGAAATTCTTTATATCTGTGCCTGCTACTCACTTGAACTGTGTGCTTCTGTAATAGGTACCGTGCCATCACTAGTGCTTACTACCGAGGAGCTGTCGGTGCTTTACTTGTCTATGATGTCACCAGGCGTGCCACATTTGAGAACGCTGCCAGGTGGTTGAAAGAGTTGCGAGATCACACAGACCCTAATATTGTGGTCATGCTTATTGGAAATAAGTCTGATCTCCGACACCTTGTTGCTGTCCCAACAGAAGATGGAAAGTCTTTTGCAGAGAGGGAGTCTCTCTACTTCATGGAGACTTCTGCCTTGGAAGCAACCAATGTAGAAAATGCATTCACTGAGGTTCTTTCTCAGATATACCGCATAGTGAGCAAGAAAGCAGTTGAAGCGGGTGAAGGTGGCAGTTCCTCGTCAGTCCCGTCTGTAGGACAGACAATAAACGTGAAAGAGGATTCTTCGGTTTTGAAGAGATTTGGATGCTGCTCGAACTAG |
| Protein sequence |
>MS.gene073174.t1 MAGYRADDEYDYLFKLVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATKSLTVDAKVIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDVTRRATFENAARWLKELRDHTDPNIVVMLIGNKSDLRHLVAVPTEDGKSFAERESLYFMETSALEATNVENAFTEVLSQIYRIVSKKAVEAGEGGSSSSVPSVGQTINVKEDSSVLKRFGCCSN |
