AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26196

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26196.t1 MTR_5g076590 100.000 182 0 0 1 182 19 200 6.38e-136 378
MS.gene26196.t1 MTR_3g064390 93.407 182 12 0 1 182 19 200 2.10e-129 361
MS.gene26196.t1 MTR_8g103550 66.484 182 60 1 1 182 18 198 3.78e-90 262
MS.gene26196.t1 MTR_2g102060 61.538 182 69 1 1 182 19 199 5.48e-83 244
MS.gene26196.t1 MTR_3g064390 85.606 132 19 0 1 132 19 150 1.45e-80 236
MS.gene26196.t1 MTR_1g094220 64.516 155 54 1 2 155 41 195 5.57e-72 216
MS.gene26196.t1 MTR_1g094220 61.392 158 56 2 2 154 41 198 2.71e-67 204
MS.gene26196.t1 MTR_1g094220 62.092 153 56 2 2 153 41 192 5.92e-66 202
MS.gene26196.t1 MTR_1g094220 63.699 146 52 1 2 146 41 186 8.07e-66 201
MS.gene26196.t1 MTR_8g031660 37.500 192 110 1 1 182 15 206 1.15e-46 152
MS.gene26196.t1 MTR_6g021790 44.737 152 84 0 2 153 16 167 7.23e-46 150
MS.gene26196.t1 MTR_4g064897 41.899 179 96 2 2 175 22 197 4.26e-45 148
MS.gene26196.t1 MTR_3g087160 42.763 152 87 0 3 154 20 171 5.08e-45 147
MS.gene26196.t1 MTR_8g090215 40.828 169 98 1 3 169 20 188 7.17e-45 147
MS.gene26196.t1 MTR_4g079350 37.629 194 108 2 2 182 23 216 9.23e-45 147
MS.gene26196.t1 MTR_8g089985 40.513 195 99 4 4 181 29 223 9.73e-45 147
MS.gene26196.t1 MTR_3g069510 40.722 194 102 3 2 182 22 215 1.02e-44 147
MS.gene26196.t1 MTR_8g031870 42.105 152 88 0 2 153 16 167 2.41e-44 145
MS.gene26196.t1 MTR_4g088090 38.144 194 107 2 2 182 23 216 4.70e-44 145
MS.gene26196.t1 MTR_2g014990 37.629 194 108 2 2 182 23 216 5.29e-44 145
MS.gene26196.t1 MTR_6g079550 38.660 194 106 2 2 182 22 215 8.38e-44 145
MS.gene26196.t1 MTR_2g075950 38.889 198 100 3 2 182 23 216 1.00e-43 145
MS.gene26196.t1 MTR_2g005510 47.586 145 76 0 2 146 25 169 3.19e-43 144
MS.gene26196.t1 MTR_8g031680 41.558 154 90 0 2 155 16 169 3.83e-43 143
MS.gene26196.t1 MTR_2g027660 42.763 152 87 0 2 153 16 167 4.95e-43 143
MS.gene26196.t1 MTR_3g086980 40.722 194 99 4 4 181 28 221 5.37e-43 143
MS.gene26196.t1 MTR_8g027220 41.765 170 94 1 3 167 37 206 1.77e-42 142
MS.gene26196.t1 MTR_2g083860 42.857 154 88 0 2 155 23 176 2.44e-42 141
MS.gene26196.t1 MTR_5g013900 38.554 166 102 0 4 169 21 186 3.91e-42 140
MS.gene26196.t1 MTR_7g081700 38.587 184 110 1 2 182 18 201 4.90e-42 140
MS.gene26196.t1 MTR_8g036220 46.207 145 78 0 2 146 26 170 6.39e-42 140
MS.gene26196.t1 MTR_8g031830 37.368 190 105 4 1 182 15 198 1.11e-41 139
MS.gene26196.t1 MTR_4g098950 40.397 151 90 0 4 154 21 171 1.55e-41 139
MS.gene26196.t1 MTR_1g078200 37.374 198 105 3 4 182 26 223 1.73e-41 139
MS.gene26196.t1 MTR_0262s0050 37.306 193 106 2 4 181 27 219 6.49e-41 137
MS.gene26196.t1 MTR_1g068730 36.649 191 109 4 2 181 25 214 6.61e-41 137
MS.gene26196.t1 MTR_1g052475 40.237 169 93 1 4 164 27 195 1.42e-40 137
MS.gene26196.t1 MTR_1g068740 36.649 191 109 4 2 181 25 214 1.49e-40 136
MS.gene26196.t1 MTR_8g006970 39.037 187 106 3 2 182 32 216 2.16e-40 136
MS.gene26196.t1 MTR_4g099470 40.351 171 95 3 17 182 9 177 3.18e-40 134
MS.gene26196.t1 MTR_2g005500 40.994 161 94 1 4 164 21 180 4.73e-40 135
MS.gene26196.t1 MTR_7g051940 37.436 195 102 4 4 182 25 215 1.26e-39 134
MS.gene26196.t1 MTR_1g115325 37.368 190 109 4 2 181 25 214 1.94e-39 134
MS.gene26196.t1 MTR_2g090865 40.789 152 90 0 2 153 21 172 2.81e-39 133
MS.gene26196.t1 MTR_8g006970 42.953 149 85 0 2 150 18 166 1.09e-38 130
MS.gene26196.t1 MTR_7g100020 40.000 155 92 1 2 155 25 179 2.04e-38 131
MS.gene26196.t1 MTR_7g405790 34.536 194 112 2 4 182 27 220 5.27e-38 130
MS.gene26196.t1 MTR_8g093740 37.968 187 108 4 2 181 23 208 1.13e-35 124
MS.gene26196.t1 MTR_4g063160 35.602 191 112 4 2 181 25 215 1.27e-35 124
MS.gene26196.t1 MTR_2g096550 32.447 188 119 4 2 181 25 212 1.24e-34 121
MS.gene26196.t1 MTR_8g107070 44.595 148 82 0 2 149 24 171 1.40e-34 122
MS.gene26196.t1 MTR_7g103070 37.368 190 109 4 2 182 26 214 1.09e-33 119
MS.gene26196.t1 MTR_3g096350 36.898 187 110 5 2 181 22 207 2.44e-33 118
MS.gene26196.t1 MTR_8g092020 35.484 186 113 4 2 180 22 207 5.18e-33 117
MS.gene26196.t1 MTR_1g029450 37.566 189 109 6 2 182 27 214 5.48e-33 117
MS.gene26196.t1 MTR_1g090717 36.508 189 111 5 2 182 25 212 1.32e-32 116
MS.gene26196.t1 MTR_7g117910 39.231 130 79 0 3 132 31 160 1.47e-32 116
MS.gene26196.t1 MTR_1g062760 42.484 153 85 3 3 153 25 176 1.90e-32 115
MS.gene26196.t1 MTR_8g093740 36.747 166 98 3 23 181 10 175 3.48e-30 108
MS.gene26196.t1 MTR_1g097420 38.854 157 89 3 2 151 18 174 5.10e-30 109
MS.gene26196.t1 MTR_4g069850 37.580 157 91 3 2 151 18 174 1.23e-29 108
MS.gene26196.t1 MTR_1g068730 37.815 119 74 0 2 120 25 143 1.01e-28 105
MS.gene26196.t1 MTR_1g097420 38.710 155 88 3 4 151 47 201 1.53e-28 106
MS.gene26196.t1 MTR_1g068740 37.815 119 74 0 2 120 25 143 1.58e-28 105
MS.gene26196.t1 MTR_2g101890 34.940 166 101 3 2 160 20 185 9.09e-28 103
MS.gene26196.t1 MTR_7g103070 37.975 158 88 4 34 182 2 158 1.86e-27 101
MS.gene26196.t1 MTR_4g012940 34.940 166 101 3 2 160 18 183 1.87e-27 102
MS.gene26196.t1 MTR_6g039490 34.395 157 96 4 2 151 18 174 1.22e-23 93.6
MS.gene26196.t1 MTR_3g107717 31.515 165 107 4 2 163 23 184 7.93e-21 85.9
MS.gene26196.t1 MTR_3g107707 31.515 165 107 4 2 163 23 184 8.53e-21 85.9
MS.gene26196.t1 MTR_3g107713 31.515 165 107 4 2 163 23 184 8.71e-21 85.9
MS.gene26196.t1 MTR_3g096350 39.450 109 63 3 2 108 22 129 3.51e-19 79.7
MS.gene26196.t1 MTR_3g096350 39.450 109 63 3 2 108 22 129 7.77e-19 79.3
MS.gene26196.t1 MTR_1452s0010 41.463 82 48 0 12 93 1 82 2.20e-18 76.6
MS.gene26196.t1 MTR_3g078260 31.646 158 90 6 3 146 19 172 1.12e-17 77.4
MS.gene26196.t1 MTR_8g075240 31.410 156 93 4 3 146 17 170 1.91e-17 76.6
MS.gene26196.t1 MTR_4g088055 29.487 156 96 4 3 146 17 170 4.75e-17 75.5
MS.gene26196.t1 MTR_5g022600 30.769 156 94 4 3 146 17 170 1.02e-16 74.7
MS.gene26196.t1 MTR_1686s0010 39.241 79 48 0 12 90 1 79 1.14e-16 71.6
MS.gene26196.t1 MTR_4g073250 29.677 155 95 4 4 146 18 170 4.04e-16 73.2
MS.gene26196.t1 MTR_6g087980 29.936 157 92 5 4 146 18 170 5.49e-16 72.8
MS.gene26196.t1 MTR_2g090875 28.387 155 97 4 4 146 18 170 2.26e-15 70.9
MS.gene26196.t1 MTR_4g113620 28.947 152 96 5 2 153 23 162 2.94e-15 70.9
MS.gene26196.t1 MTR_3g078260 32.710 107 65 4 3 106 19 121 8.77e-12 60.5
MS.gene26196.t1 MTR_5g092060 29.299 157 101 4 4 153 116 269 6.20e-11 60.1
MS.gene26196.t1 MTR_4g015360 67.568 37 12 0 118 154 21 57 8.13e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26196.t1 AT5G45130 80.769 182 35 0 1 182 19 200 5.42e-109 310
MS.gene26196.t1 AT5G45130 80.769 182 35 0 1 182 27 208 1.52e-108 309
MS.gene26196.t1 AT4G19640 79.121 182 38 0 1 182 19 200 3.49e-108 308
MS.gene26196.t1 AT4G19640 86.066 122 17 0 1 122 19 140 7.39e-74 219
MS.gene26196.t1 AT4G19640 83.871 124 20 0 1 124 19 142 1.16e-73 220
MS.gene26196.t1 AT3G54840 62.264 159 59 1 2 159 9 167 2.56e-71 213
MS.gene26196.t1 AT3G54840 62.264 159 59 1 2 159 43 201 3.42e-71 214
MS.gene26196.t1 AT3G54840 63.014 146 53 1 2 146 43 188 4.41e-65 198
MS.gene26196.t1 AT5G60860 38.144 194 107 2 2 182 23 216 1.23e-45 149
MS.gene26196.t1 AT4G39990 38.265 196 102 4 4 182 29 222 1.99e-45 149
MS.gene26196.t1 AT1G07410 41.146 192 102 3 2 182 22 213 2.17e-45 149
MS.gene26196.t1 AT4G18800 38.220 191 108 2 2 182 23 213 3.94e-45 148
MS.gene26196.t1 AT4G35860 44.737 152 84 0 2 153 16 167 5.70e-45 148
MS.gene26196.t1 AT5G45750 37.824 193 108 2 2 182 23 215 9.93e-45 147
MS.gene26196.t1 AT4G17160 44.966 149 82 0 2 150 16 164 1.82e-44 146
MS.gene26196.t1 AT1G16920 39.896 193 104 2 2 182 23 215 2.84e-44 146
MS.gene26196.t1 AT2G44610 43.709 151 85 0 4 154 21 171 5.75e-44 145
MS.gene26196.t1 AT1G09630 46.104 154 83 0 2 155 22 175 5.79e-44 145
MS.gene26196.t1 AT5G10260 42.105 152 88 0 3 154 20 171 5.96e-44 145
MS.gene26196.t1 AT2G22290 37.433 187 110 2 3 182 20 206 8.35e-44 144
MS.gene26196.t1 AT1G28550 42.208 154 89 0 2 155 23 176 1.26e-43 144
MS.gene26196.t1 AT1G06400 38.860 193 106 2 2 182 23 215 3.02e-43 143
MS.gene26196.t1 AT2G33870 42.857 154 88 0 2 155 23 176 3.67e-43 143
MS.gene26196.t1 AT4G18430 36.598 194 110 2 2 182 23 216 4.54e-43 143
MS.gene26196.t1 AT3G07410 40.933 193 100 3 4 182 24 216 4.84e-43 143
MS.gene26196.t1 AT3G15060 42.857 154 88 0 2 155 23 176 6.21e-43 142
MS.gene26196.t1 AT5G59150 40.404 198 98 4 2 182 22 216 1.06e-42 142
MS.gene26196.t1 AT3G46830 39.394 198 101 3 2 182 22 217 1.54e-42 142
MS.gene26196.t1 AT3G46830 39.394 198 101 3 2 182 22 217 1.54e-42 142
MS.gene26196.t1 AT4G17530 39.247 186 106 3 2 182 18 201 1.58e-42 141
MS.gene26196.t1 AT4G39890 40.909 154 90 1 2 154 19 172 2.14e-42 141
MS.gene26196.t1 AT4G17170 42.105 152 88 0 2 153 16 167 2.55e-42 141
MS.gene26196.t1 AT5G47200 39.130 184 109 1 2 182 18 201 4.27e-42 140
MS.gene26196.t1 AT5G47520 36.923 195 107 4 4 182 26 220 5.09e-42 140
MS.gene26196.t1 AT1G02130 39.572 187 105 3 2 182 18 202 6.56e-42 140
MS.gene26196.t1 AT5G65270 42.000 150 87 0 4 153 29 178 8.90e-42 140
MS.gene26196.t1 AT2G43130 38.421 190 106 2 4 182 24 213 2.17e-41 139
MS.gene26196.t1 AT3G12160 43.421 152 86 0 4 155 27 178 3.29e-41 138
MS.gene26196.t1 AT5G59840 37.500 192 106 4 2 181 25 214 3.83e-40 135
MS.gene26196.t1 AT3G11730 36.216 185 111 1 3 180 19 203 4.60e-40 135
MS.gene26196.t1 AT5G47960 41.139 158 93 0 4 161 27 184 5.69e-40 135
MS.gene26196.t1 AT1G05810 36.788 193 108 3 4 182 24 216 1.00e-39 134
MS.gene26196.t1 AT1G05810 36.788 193 108 3 4 182 70 262 2.00e-39 135
MS.gene26196.t1 AT3G46060 36.458 192 108 4 2 181 25 214 3.26e-39 133
MS.gene26196.t1 AT3G46060 36.458 192 108 4 2 181 25 214 3.26e-39 133
MS.gene26196.t1 AT3G46060 36.458 192 108 4 2 181 25 214 3.26e-39 133
MS.gene26196.t1 AT3G53610 35.385 195 106 4 2 181 25 214 1.42e-38 131
MS.gene26196.t1 AT3G53610 35.385 195 106 4 2 181 25 214 1.42e-38 131
MS.gene26196.t1 AT3G53610 35.385 195 106 4 2 181 25 214 1.42e-38 131
MS.gene26196.t1 AT5G64990 40.132 152 91 0 2 153 17 168 1.89e-38 131
MS.gene26196.t1 AT1G73640 44.444 144 80 0 3 146 24 167 2.70e-38 131
MS.gene26196.t1 AT2G31680 35.567 194 110 3 4 182 24 217 7.59e-38 130
MS.gene26196.t1 AT1G18200 41.722 151 88 0 3 153 24 174 8.41e-38 130
MS.gene26196.t1 AT4G35860 46.667 120 64 0 34 153 2 121 2.32e-37 127
MS.gene26196.t1 AT1G01200 42.384 151 86 1 4 153 40 190 5.38e-37 128
MS.gene26196.t1 AT5G03520 37.368 190 110 4 2 182 25 214 5.54e-36 125
MS.gene26196.t1 AT5G64990 38.365 159 91 1 2 153 17 175 1.08e-35 124
MS.gene26196.t1 AT3G09900 35.938 192 112 3 2 182 25 216 1.75e-35 124
MS.gene26196.t1 AT5G59150 40.361 166 79 4 34 182 3 165 2.81e-33 116
MS.gene26196.t1 AT1G22740 39.490 157 88 3 2 151 18 174 6.92e-33 116
MS.gene26196.t1 AT5G03530 38.421 190 106 6 2 182 23 210 8.02e-32 114
MS.gene26196.t1 AT5G03530 38.421 190 106 6 2 182 23 210 8.02e-32 114
MS.gene26196.t1 AT1G43890 40.260 154 89 3 2 153 23 175 8.99e-32 114
MS.gene26196.t1 AT1G43890 40.260 154 89 3 2 153 23 175 8.99e-32 114
MS.gene26196.t1 AT1G43890 40.260 154 89 3 2 153 23 175 8.99e-32 114
MS.gene26196.t1 AT5G03520 36.196 163 95 4 29 182 42 204 5.53e-31 112
MS.gene26196.t1 AT2G21880 37.179 156 91 3 2 150 19 174 1.50e-29 108
MS.gene26196.t1 AT3G18820 36.943 157 92 3 2 151 18 174 1.87e-29 108
MS.gene26196.t1 AT1G52280 35.119 168 102 3 2 162 18 185 2.50e-29 107
MS.gene26196.t1 AT4G09720 38.854 157 89 3 2 151 18 174 1.26e-28 105
MS.gene26196.t1 AT3G16100 35.542 166 100 3 2 160 18 183 1.85e-28 105
MS.gene26196.t1 AT5G39620 36.076 158 94 3 2 152 15 172 6.74e-28 103
MS.gene26196.t1 AT3G09910 34.783 161 103 2 24 182 44 204 7.61e-28 103
MS.gene26196.t1 AT3G09910 34.783 161 103 2 24 182 44 204 7.61e-28 103
MS.gene26196.t1 AT3G09910 34.783 161 103 2 24 182 44 204 7.61e-28 103
MS.gene26196.t1 AT1G49300 36.538 156 92 3 2 150 18 173 2.32e-27 102
MS.gene26196.t1 AT1G49300 36.538 156 92 3 2 150 18 173 2.32e-27 102
MS.gene26196.t1 AT3G09910 38.806 134 80 2 24 155 44 177 3.07e-26 99.4
MS.gene26196.t1 AT3G09910 38.806 134 80 2 24 155 44 177 3.07e-26 99.4
MS.gene26196.t1 AT4G09720 37.654 162 89 4 2 151 18 179 4.67e-26 99.4
MS.gene26196.t1 AT2G21880 36.735 147 86 3 11 150 3 149 6.28e-26 98.6
MS.gene26196.t1 AT2G21880 36.735 147 86 3 11 150 3 149 6.28e-26 98.6
MS.gene26196.t1 AT4G09720 36.310 168 89 4 2 151 18 185 4.85e-25 97.1
MS.gene26196.t1 AT4G09720 39.416 137 76 3 22 151 4 140 1.11e-24 94.7
MS.gene26196.t1 AT4G09720 39.416 137 76 3 22 151 4 140 1.11e-24 94.7
MS.gene26196.t1 AT4G09720 39.416 137 76 3 22 151 4 140 1.11e-24 94.7
MS.gene26196.t1 AT5G55080 29.605 152 104 3 2 153 23 171 6.07e-22 89.0
MS.gene26196.t1 AT5G55190 32.237 152 100 3 2 153 23 171 1.54e-20 85.1
MS.gene26196.t1 AT5G20010 32.237 152 100 3 2 153 23 171 1.81e-20 85.1
MS.gene26196.t1 AT5G20020 32.237 152 100 3 2 153 23 171 1.81e-20 85.1
MS.gene26196.t1 AT2G21880 32.051 156 91 4 2 150 19 166 9.08e-20 82.8
MS.gene26196.t1 AT5G46025 38.710 93 56 1 62 153 14 106 6.77e-18 75.5
MS.gene26196.t1 AT3G51300 31.410 156 93 4 3 146 17 170 8.71e-18 77.4
MS.gene26196.t1 AT2G44690 32.298 161 95 5 3 151 29 187 1.33e-17 77.4
MS.gene26196.t1 AT2G17800 32.051 156 92 4 3 146 17 170 1.43e-17 77.0
MS.gene26196.t1 AT2G17800 32.051 156 92 4 3 146 17 170 1.43e-17 77.0
MS.gene26196.t1 AT4G28950 30.968 155 91 5 4 146 18 168 1.86e-17 77.0
MS.gene26196.t1 AT4G35950 32.051 156 92 5 3 146 17 170 4.38e-17 75.5
MS.gene26196.t1 AT3G48040 29.114 158 94 5 3 146 19 172 1.84e-16 74.3
MS.gene26196.t1 AT4G35020 31.613 155 92 5 4 146 18 170 2.96e-16 73.6
MS.gene26196.t1 AT4G35020 31.613 155 92 5 4 146 18 170 2.96e-16 73.6
MS.gene26196.t1 AT4G35020 31.613 155 92 5 4 146 18 170 2.96e-16 73.6
MS.gene26196.t1 AT5G62880 31.013 158 91 6 3 146 19 172 5.91e-16 73.2
MS.gene26196.t1 AT3G51290 34.109 129 72 3 30 146 644 771 9.82e-16 74.7
MS.gene26196.t1 AT1G75840 30.128 156 95 5 3 146 17 170 1.01e-15 72.0
MS.gene26196.t1 AT1G20090 30.128 156 95 5 3 146 16 169 1.41e-15 71.6
MS.gene26196.t1 AT5G45970 28.846 156 97 4 3 146 17 170 2.43e-15 71.2
MS.gene26196.t1 AT3G48040 28.667 150 89 5 3 138 19 164 1.85e-14 68.2
MS.gene26196.t1 AT5G62880 30.667 150 86 6 3 138 19 164 5.61e-14 67.4

Find 41 sgRNAs with CRISPR-Local

Find 270 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTCAATTCCTTGAATTTC+AGG 0.172043 5.4:-57828318 MS.gene26196:intron
CCACAGTGAAATTTGAGATT+TGG 0.262162 5.4:-57826740 MS.gene26196:CDS
TTGTGCCCTTGGTAACTTCT+TGG 0.276630 5.4:+57823550 None:intergenic
CACAGTGAAATTTGAGATTT+GGG 0.299158 5.4:-57826739 MS.gene26196:CDS
CGTCATTTACTGCCAACGTC+TGG 0.316274 5.4:+57826763 None:intergenic
GTGAAACAGGAATCAACAAT+AGG 0.375057 5.4:-57826801 MS.gene26196:intron
CATTTACACGAGCGAAGAAA+TGG 0.391385 5.4:-57826408 MS.gene26196:CDS
CGTGTATATGCTGAGGAAAA+TGG 0.414206 5.4:-57824532 MS.gene26196:CDS
GCAAAAGCCACGACCATGTT+CGG 0.437301 5.4:+57825821 None:intergenic
TGAAACAGGAATCAACAATA+GGG 0.443425 5.4:-57826800 MS.gene26196:intron
TACCTTGCTTCTGAAGCTCT+TGG 0.443949 5.4:+57826383 None:intergenic
CAACCAGCGCAAAACCCAGC+AGG 0.448579 5.4:-57823530 MS.gene26196:CDS
CTTCTTGGTGATATGGGTGC+TGG 0.450654 5.4:-57828371 None:intergenic
GCAGCTCTGGTTCCTTCGGC+AGG 0.470619 5.4:+57823488 None:intergenic
TGTCTATGATATTACTAACT+TGG 0.471350 5.4:-57826646 MS.gene26196:intron
TGCTGGTAACAAAGCTGACT+TGG 0.472663 5.4:-57825801 MS.gene26196:CDS
ATAAAATATGTCATTAACAT+TGG 0.476631 5.4:+57824474 None:intergenic
TCAACAAGAACCATCCCTGC+TGG 0.485332 5.4:+57823515 None:intergenic
TGAACAACATGATGCAGCTC+TGG 0.499235 5.4:+57823475 None:intergenic
AAGCTGACTTGGAAGATAAG+AGG 0.510951 5.4:-57825790 MS.gene26196:CDS
TGATGCAGCTCTGGTTCCTT+CGG 0.524363 5.4:+57823484 None:intergenic
GTCATTTACTGCCAACGTCT+GGG 0.532033 5.4:+57826764 None:intergenic
TTAGCTCCCATGTACTATAG+AGG 0.537219 5.4:-57826687 MS.gene26196:CDS
TTTGAGATTTGGGACACAGC+AGG 0.538081 5.4:-57826729 MS.gene26196:CDS
AGCGCAAAACCCAGCAGGGA+TGG 0.543975 5.4:-57823525 MS.gene26196:CDS
ATTTACACGAGCGAAGAAAT+GGG 0.562436 5.4:-57826407 MS.gene26196:CDS
AGCAGCGCCTCTATAGTACA+TGG 0.565513 5.4:+57826680 None:intergenic
CAACAAGAACCATCCCTGCT+GGG 0.567898 5.4:+57823516 None:intergenic
AGGGAATCCGAACATGGTCG+TGG 0.578957 5.4:-57825828 MS.gene26196:CDS
TGTTGCAGGGAATCCGAACA+TGG 0.580245 5.4:-57825834 MS.gene26196:intron
AACCAGCGCAAAACCCAGCA+GGG 0.586823 5.4:-57823529 MS.gene26196:CDS
CCTTAACAAAACGCAAAACA+AGG 0.590364 5.4:+57828339 None:intergenic
AGTACATGGGAGCTAAGCTA+TGG 0.598496 5.4:+57826694 None:intergenic
GTCCAAGAGCTTCAGAAGCA+AGG 0.599811 5.4:-57826385 MS.gene26196:intron
CTTGTTGATAGACCTGCCGA+AGG 0.652869 5.4:-57823500 MS.gene26196:CDS
GGAAGCACGTGTATATGCTG+AGG 0.658380 5.4:-57824539 MS.gene26196:CDS
GAAACAGGAATCAACAATAG+GGG 0.660375 5.4:-57826799 MS.gene26196:intron
GAACAGCCAAGAAGTTACCA+AGG 0.663916 5.4:-57823556 MS.gene26196:intron
AACAGCCAAGAAGTTACCAA+GGG 0.689870 5.4:-57823555 MS.gene26196:intron
GCAGCGCCTCTATAGTACAT+GGG 0.698335 5.4:+57826681 None:intergenic
CCAAATCTCAAATTTCACTG+TGG 0.747675 5.4:+57826740 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAAAGATAAATCAAA+AGG + chr5.4:57823547-57823566 None:intergenic 10.0%
!!! AAAAATTTTAGATTGTAAAA+AGG - chr5.4:57825525-57825544 MS.gene26196:intron 10.0%
!!! ATTTATCTTTTAAATTCAAT+TGG - chr5.4:57823551-57823570 MS.gene26196:CDS 10.0%
!!! TTTATCTTTTAAATTCAATT+GGG - chr5.4:57823552-57823571 MS.gene26196:CDS 10.0%
!! AAACAATAGCTAAAAAATTT+CGG + chr5.4:57825873-57825892 None:intergenic 15.0%
!! ATAAAATATGTCATTAACAT+TGG + chr5.4:57827360-57827379 None:intergenic 15.0%
!! ATCTTATATTATAGAATGTA+TGG - chr5.4:57823917-57823936 MS.gene26196:intron 15.0%
!! ATGATATTTAAGTGTAATAA+TGG - chr5.4:57828131-57828150 MS.gene26196:intron 15.0%
!! ATTAATATATGAATTGTGAA+CGG - chr5.4:57825757-57825776 MS.gene26196:intron 15.0%
!! TAAGAAGAAATATTAGAATA+TGG + chr5.4:57825614-57825633 None:intergenic 15.0%
!! TATGTTAATTTATTTGTAGA+CGG + chr5.4:57824409-57824428 None:intergenic 15.0%
!! TTATATATAGTTTATTACTG+TGG - chr5.4:57825360-57825379 MS.gene26196:intron 15.0%
!!! AAGAAGAAACAATTTTATTT+AGG + chr5.4:57824070-57824089 None:intergenic 15.0%
!!! AATGACATATTTTATGAAAT+AGG - chr5.4:57827365-57827384 MS.gene26196:intron 15.0%
!!! GAAAACTAAACTATTTATTT+TGG - chr5.4:57825547-57825566 MS.gene26196:intron 15.0%
!!! TTTTTGACTAAATTGAAATT+GGG - chr5.4:57823701-57823720 MS.gene26196:intron 15.0%
!! AAATTCATTCACACAAATAA+TGG - chr5.4:57828164-57828183 MS.gene26196:intron 20.0%
!! TGTAGAATTCAAAATTTATG+AGG - chr5.4:57823579-57823598 MS.gene26196:intron 20.0%
!! TTATATTATAGAATGTATGG+TGG - chr5.4:57823920-57823939 MS.gene26196:intron 20.0%
!! TTTACTTAAAAGTTCAAACT+GGG - chr5.4:57824568-57824587 MS.gene26196:intron 20.0%
!!! CATAGCATGTTATATTTTTT+GGG - chr5.4:57824501-57824520 MS.gene26196:CDS 20.0%
!!! GTTTTTGACTAAATTGAAAT+TGG - chr5.4:57823700-57823719 MS.gene26196:intron 20.0%
!!! TAAGATGGTATTTTCAAATT+TGG - chr5.4:57828107-57828126 MS.gene26196:intron 20.0%
!!! TATTTTGTATATAGTAGTGT+GGG - chr5.4:57824905-57824924 MS.gene26196:intron 20.0%
!!! TCATAGCATGTTATATTTTT+TGG - chr5.4:57824500-57824519 MS.gene26196:CDS 20.0%
!!! TCCTATATTTTTTACATTAG+CGG - chr5.4:57824447-57824466 MS.gene26196:intron 20.0%
!!! TCTTATTCTTACATTTTTTC+TGG - chr5.4:57825494-57825513 MS.gene26196:intron 20.0%
!!! TTATTTTGTATATAGTAGTG+TGG - chr5.4:57824904-57824923 MS.gene26196:intron 20.0%
!!! TTTTGACTAAATTGAAATTG+GGG - chr5.4:57823702-57823721 MS.gene26196:intron 20.0%
! AACTAACCCATGATATTTAA+TGG - chr5.4:57825844-57825863 MS.gene26196:intron 25.0%
! AAGAGCTTCTTAAGAAATTA+AGG - chr5.4:57826684-57826703 MS.gene26196:CDS 25.0%
! AATTTAGCAGAAAAAATGTG+TGG + chr5.4:57826177-57826196 None:intergenic 25.0%
! ACATAATTTATATCAGTCCA+AGG - chr5.4:57827492-57827511 MS.gene26196:intron 25.0%
! ACCGCTAATGTAAAAAATAT+AGG + chr5.4:57824451-57824470 None:intergenic 25.0%
! AGTCAATCTGTTTAAAGAAT+TGG + chr5.4:57824974-57824993 None:intergenic 25.0%
! ATAAACTCCTTGAATAAAGT+GGG - chr5.4:57826904-57826923 MS.gene26196:intron 25.0%
! ATTGTACTCTCAATATAAGA+TGG - chr5.4:57828092-57828111 MS.gene26196:intron 25.0%
! ATTGTACTTCAAAGTTATAG+TGG - chr5.4:57825929-57825948 MS.gene26196:intron 25.0%
! CAATAAATAACAACAGATCT+CGG + chr5.4:57824951-57824970 None:intergenic 25.0%
! CTAAAATAATGAACTCATTC+TGG - chr5.4:57825971-57825990 MS.gene26196:intron 25.0%
! CTTTACTTAAAAGTTCAAAC+TGG - chr5.4:57824567-57824586 MS.gene26196:intron 25.0%
! GAAAAAAAACCTGAAATTCA+AGG + chr5.4:57823525-57823544 None:intergenic 25.0%
! GCAACTGTTTAAATACATAT+AGG + chr5.4:57827192-57827211 None:intergenic 25.0%
! GGTTAGATAAATGTACATAA+AGG - chr5.4:57827583-57827602 MS.gene26196:intron 25.0%
! TAAAGTGTATGTAATAGGAA+AGG + chr5.4:57827398-57827417 None:intergenic 25.0%
! TATAAACTCCTTGAATAAAG+TGG - chr5.4:57826903-57826922 MS.gene26196:intron 25.0%
! TATAGTTTCTTCAACTACAT+TGG - chr5.4:57826130-57826149 MS.gene26196:intron 25.0%
! TGAGAAATGTTGTTCAATTA+GGG - chr5.4:57824223-57824242 MS.gene26196:intron 25.0%
! TGAGTTATCAAACATTCATT+TGG - chr5.4:57824601-57824620 MS.gene26196:intron 25.0%
! TGTCTATGATATTACTAACT+TGG - chr5.4:57825185-57825204 MS.gene26196:intron 25.0%
! TTATAAGAGAAGCTATTAGT+TGG - chr5.4:57826726-57826745 MS.gene26196:CDS 25.0%
! TTGAGAAATGTTGTTCAATT+AGG - chr5.4:57824222-57824241 MS.gene26196:intron 25.0%
! TTTCCTATTACATACACTTT+AGG - chr5.4:57827397-57827416 MS.gene26196:intron 25.0%
!! AGAATGAATTGTTATGGTTT+TGG - chr5.4:57823824-57823843 MS.gene26196:intron 25.0%
!! GAATTTTATTATTCCTAGTC+TGG - chr5.4:57827621-57827640 MS.gene26196:intron 25.0%
!! TAGTCTTAAGTAAGAGAAAA+TGG - chr5.4:57828058-57828077 MS.gene26196:intron 25.0%
!! TGCAAACAACACAAATATTT+TGG + chr5.4:57827558-57827577 None:intergenic 25.0%
!! TGCTAAATTTTCAAATACCA+GGG - chr5.4:57826188-57826207 MS.gene26196:intron 25.0%
!!! TTTTTTAAATACATCGAGAG+AGG + chr5.4:57828202-57828221 None:intergenic 25.0%
AAATTAACGCAAAGTTCAGT+AGG + chr5.4:57825822-57825841 None:intergenic 30.0%
AAGTACAATTACAACCAGAA+TGG + chr5.4:57825918-57825937 None:intergenic 30.0%
AATATAGCATAGCACAAACA+TGG - chr5.4:57825702-57825721 MS.gene26196:intron 30.0%
ACTGCTTTGCTGAATTTATT+TGG - chr5.4:57825001-57825020 MS.gene26196:intron 30.0%
AGAAATGTTGTTCAATTAGG+GGG - chr5.4:57824225-57824244 MS.gene26196:intron 30.0%
AGTACAATTACAACCAGAAT+GGG + chr5.4:57825917-57825936 None:intergenic 30.0%
AGTTCTATTCTGAACATGAT+AGG - chr5.4:57823792-57823811 MS.gene26196:intron 30.0%
ATATAACATGCTATGAAGCA+TGG + chr5.4:57824496-57824515 None:intergenic 30.0%
ATTCACCTAACCAAGTTATT+TGG - chr5.4:57824662-57824681 MS.gene26196:intron 30.0%
ATTTATGAGGACAATTTCCA+TGG - chr5.4:57823592-57823611 MS.gene26196:intron 30.0%
CAACCTAAAGTGTATGTAAT+AGG + chr5.4:57827403-57827422 None:intergenic 30.0%
CAACTTTAACCCAAATAACT+TGG + chr5.4:57824675-57824694 None:intergenic 30.0%
CATAAAAAAACACATTCGTC+TGG + chr5.4:57827702-57827721 None:intergenic 30.0%
CGGTAACCATTAAATATCAT+GGG + chr5.4:57825853-57825872 None:intergenic 30.0%
CTCGATTACAAAATCATAAC+AGG + chr5.4:57823732-57823751 None:intergenic 30.0%
GAGAAATGTTGTTCAATTAG+GGG - chr5.4:57824224-57824243 MS.gene26196:intron 30.0%
GCCAAAAAAAATTTATCTCC+CGG - chr5.4:57824765-57824784 MS.gene26196:intron 30.0%
GTTGTGAGAATGAATTGTTA+TGG - chr5.4:57823818-57823837 MS.gene26196:intron 30.0%
TAACATATATGTAGCAGTGT+CGG - chr5.4:57824422-57824441 MS.gene26196:intron 30.0%
TATATGAATTGTGAACGGTT+AGG - chr5.4:57825762-57825781 MS.gene26196:intron 30.0%
TATTTGGTCTATTGTGAAAC+AGG - chr5.4:57825017-57825036 MS.gene26196:intron 30.0%
TCGGTAACCATTAAATATCA+TGG + chr5.4:57825854-57825873 None:intergenic 30.0%
TGAAACAGGAATCAACAATA+GGG - chr5.4:57825031-57825050 MS.gene26196:intron 30.0%
TTAAAGTTGTTCATGAACTC+CGG - chr5.4:57824686-57824705 MS.gene26196:intron 30.0%
TTAACCCAAATAACTTGGTT+AGG + chr5.4:57824670-57824689 None:intergenic 30.0%
TTCAATGTACAAGCTAAAGT+TGG + chr5.4:57827049-57827068 None:intergenic 30.0%
TTCACCTAACCAAGTTATTT+GGG - chr5.4:57824663-57824682 MS.gene26196:intron 30.0%
TTTATGAGGACAATTTCCAT+GGG - chr5.4:57823593-57823612 MS.gene26196:intron 30.0%
TTTCCTATACCTGTTACAAA+AGG - chr5.4:57825890-57825909 MS.gene26196:intron 30.0%
! AAATGGAAACTCTTCTTTTG+AGG - chr5.4:57827527-57827546 MS.gene26196:intron 30.0%
! AAATTTTCAAATACCAGGGA+TGG - chr5.4:57826192-57826211 MS.gene26196:intron 30.0%
! AGCTTATGCGATTTTCATTT+TGG + chr5.4:57826997-57827016 None:intergenic 30.0%
! CACAGTGAAATTTGAGATTT+GGG - chr5.4:57825092-57825111 MS.gene26196:intron 30.0%
! CTGCTAAATTTTCAAATACC+AGG - chr5.4:57826187-57826206 MS.gene26196:intron 30.0%
! TGATTATTTCTTCTGTTCAC+AGG - chr5.4:57825397-57825416 MS.gene26196:intron 30.0%
!! CATGTTGATAGTTCTACTAT+CGG - chr5.4:57826844-57826863 MS.gene26196:intron 30.0%
!! CTTTAAACAGATTGACTTGT+GGG - chr5.4:57824977-57824996 MS.gene26196:intron 30.0%
!! TCTTTAAACAGATTGACTTG+TGG - chr5.4:57824976-57824995 MS.gene26196:intron 30.0%
!!! ACCGGGAGATAAATTTTTTT+TGG + chr5.4:57824769-57824788 None:intergenic 30.0%
!!! ATGAATTGTTATGGTTTTGG+AGG - chr5.4:57823827-57823846 MS.gene26196:intron 30.0%
!!! CTTGTTTTGCGTTTTGTTAA+GGG - chr5.4:57823493-57823512 MS.gene26196:CDS 30.0%
!!! GCATGTTATATTTTTTGGGT+TGG - chr5.4:57824505-57824524 MS.gene26196:CDS 30.0%
!!! GTGTCATTTTCCTTTCTTTA+GGG - chr5.4:57825290-57825309 MS.gene26196:intron 30.0%
!!! TGTGTCATTTTCCTTTCTTT+AGG - chr5.4:57825289-57825308 MS.gene26196:intron 30.0%
!!! TTATTTTTGTCTTAACCCTC+CGG + chr5.4:57824827-57824846 None:intergenic 30.0%
AATCCTTTGTACAACCTGAT+TGG - chr5.4:57826369-57826388 MS.gene26196:intron 35.0%
ACTCTTGTATTTACAGTGTG+TGG + chr5.4:57824192-57824211 None:intergenic 35.0%
ACTTGAAGAAGCTCAGATAT+AGG + chr5.4:57826493-57826512 None:intergenic 35.0%
ACTTGAAGAATCTTCTGTGT+TGG + chr5.4:57827121-57827140 None:intergenic 35.0%
AGAACATAAATGTCCCAATC+AGG + chr5.4:57826386-57826405 None:intergenic 35.0%
AGAATCTTCTGTGTTGGAAA+TGG + chr5.4:57827115-57827134 None:intergenic 35.0%
ATACACAAAGCTATAGAGAC+TGG + chr5.4:57826272-57826291 None:intergenic 35.0%
ATACACTTACTACGTCACAA+GGG + chr5.4:57826644-57826663 None:intergenic 35.0%
ATCCTTTGTACAACCTGATT+GGG - chr5.4:57826370-57826389 MS.gene26196:intron 35.0%
ATGAGAAACTACTTGTTTCG+AGG - chr5.4:57826547-57826566 MS.gene26196:intron 35.0%
ATTTACACGAGCGAAGAAAT+GGG - chr5.4:57825424-57825443 MS.gene26196:intron 35.0%
CAGTTTCACTATAGCATCAT+AGG - chr5.4:57826104-57826123 MS.gene26196:intron 35.0%
CATAAATGTAACTGGCACAT+CGG + chr5.4:57827230-57827249 None:intergenic 35.0%
CCAAATCTCAAATTTCACTG+TGG + chr5.4:57825094-57825113 None:intergenic 35.0%
CCTACAACTGCTTATTGAAA+AGG - chr5.4:57826797-57826816 MS.gene26196:intron 35.0%
CCTTAACAAAACGCAAAACA+AGG + chr5.4:57823495-57823514 None:intergenic 35.0%
CTTCGTATAAGACTGAAGAA+GGG - chr5.4:57827753-57827772 MS.gene26196:intron 35.0%
GAAACAGGAATCAACAATAG+GGG - chr5.4:57825032-57825051 MS.gene26196:intron 35.0%
GATGTGCCAGTTACATTTAT+GGG - chr5.4:57827229-57827248 MS.gene26196:intron 35.0%
GCACTAAAATATTCCAAGCA+TGG + chr5.4:57825331-57825350 None:intergenic 35.0%
GTAGTTCCCATAAATGTAAC+TGG + chr5.4:57827238-57827257 None:intergenic 35.0%
GTGAAACAGGAATCAACAAT+AGG - chr5.4:57825030-57825049 MS.gene26196:intron 35.0%
TACACAAAGCTATAGAGACT+GGG + chr5.4:57826271-57826290 None:intergenic 35.0%
TATACACTTACTACGTCACA+AGG + chr5.4:57826645-57826664 None:intergenic 35.0%
TCTCATTCTGTTTAGTGAGA+CGG + chr5.4:57827933-57827952 None:intergenic 35.0%
TCTTCGTATAAGACTGAAGA+AGG - chr5.4:57827752-57827771 MS.gene26196:intron 35.0%
TTATCAAATGAAACCATCCC+TGG + chr5.4:57826208-57826227 None:intergenic 35.0%
TTGGTTAAACCTAAAACCCA+TGG + chr5.4:57823612-57823631 None:intergenic 35.0%
TTGTGACGTAGTAAGTGTAT+AGG - chr5.4:57826644-57826663 MS.gene26196:intron 35.0%
TTTATATCAGTCCAAGGAAG+AGG - chr5.4:57827498-57827517 MS.gene26196:intron 35.0%
! ACTTTTATGCTGCTACGTTT+TGG - chr5.4:57826227-57826246 MS.gene26196:intron 35.0%
! CCACAGTGAAATTTGAGATT+TGG - chr5.4:57825091-57825110 MS.gene26196:intron 35.0%
! CCTTTTCAATAAGCAGTTGT+AGG + chr5.4:57826800-57826819 None:intergenic 35.0%
! CTTTTATGCTGCTACGTTTT+GGG - chr5.4:57826228-57826247 MS.gene26196:intron 35.0%
! CTTTTCTAACCATAACCAAC+CGG - chr5.4:57826298-57826317 MS.gene26196:intron 35.0%
! GTTCACTCATGAACGTTTTA+TGG + chr5.4:57827795-57827814 None:intergenic 35.0%
!! ACAAGTTTTGTCACTCTGTA+GGG - chr5.4:57827889-57827908 MS.gene26196:intron 35.0%
!! ATTTGTGTTGTTTGCACTAC+TGG - chr5.4:57827562-57827581 MS.gene26196:intron 35.0%
!! GAAGCAAGGTATTTTCTGAT+TGG - chr5.4:57825460-57825479 MS.gene26196:intron 35.0%
!! TACAAGTTTTGTCACTCTGT+AGG - chr5.4:57827888-57827907 MS.gene26196:intron 35.0%
!!! ATGGTTTTGGAGGTTTGAAT+GGG - chr5.4:57823837-57823856 MS.gene26196:intron 35.0%
!!! CCTTGTTTTGCGTTTTGTTA+AGG - chr5.4:57823492-57823511 MS.gene26196:CDS 35.0%
!!! TATGGTTTTGGAGGTTTGAA+TGG - chr5.4:57823836-57823855 MS.gene26196:intron 35.0%
AACAGCCAAGAAGTTACCAA+GGG - chr5.4:57828276-57828295 MS.gene26196:intron 40.0%
AAGAATCCGAGTTTGAATCC+TGG - chr5.4:57824728-57824747 MS.gene26196:intron 40.0%
AATATTCCAAGCATGGTCTC+TGG + chr5.4:57825324-57825343 None:intergenic 40.0%
AATCCAGCCCACTTTATTCA+AGG + chr5.4:57826914-57826933 None:intergenic 40.0%
ACAGCTCGTATGAGTTACAT+AGG + chr5.4:57824125-57824144 None:intergenic 40.0%
AGAATGTATGGTGGTACTTG+TGG - chr5.4:57823929-57823948 MS.gene26196:intron 40.0%
AGTGTATAGGTGGGATTTGT+TGG - chr5.4:57826657-57826676 MS.gene26196:CDS 40.0%
ATTGTACAAGAGTCATGAGC+TGG + chr5.4:57827269-57827288 None:intergenic 40.0%
CAAGGAAGAGGAAGAGAAAA+TGG - chr5.4:57827510-57827529 MS.gene26196:intron 40.0%
CATTTACACGAGCGAAGAAA+TGG - chr5.4:57825423-57825442 MS.gene26196:intron 40.0%
CCAAATAGTTCGCTCTTAAC+CGG + chr5.4:57824708-57824727 None:intergenic 40.0%
CGATGTGCCAGTTACATTTA+TGG - chr5.4:57827228-57827247 MS.gene26196:intron 40.0%
CGTATGAGTTACATAGGTGT+CGG + chr5.4:57824119-57824138 None:intergenic 40.0%
CGTGTATATGCTGAGGAAAA+TGG - chr5.4:57827299-57827318 MS.gene26196:intron 40.0%
GAATGTATGGTGGTACTTGT+GGG - chr5.4:57823930-57823949 MS.gene26196:intron 40.0%
GACGTAGTAAGTGTATAGGT+GGG - chr5.4:57826648-57826667 MS.gene26196:CDS 40.0%
GGGTCAATTCCTTGAATTTC+AGG - chr5.4:57823513-57823532 MS.gene26196:CDS 40.0%
TATGAAGCACAAACACCTCT+AGG - chr5.4:57823972-57823991 MS.gene26196:intron 40.0%
TCATGACTCTTGTACAATGC+AGG - chr5.4:57827271-57827290 MS.gene26196:intron 40.0%
TGACGTAGTAAGTGTATAGG+TGG - chr5.4:57826647-57826666 MS.gene26196:CDS 40.0%
TGTTACAAAAGGACCCATTC+TGG - chr5.4:57825901-57825920 MS.gene26196:intron 40.0%
TTAGCTCCCATGTACTATAG+AGG - chr5.4:57825144-57825163 MS.gene26196:intron 40.0%
TTCGTCTGGTGCAAATCAAA+AGG + chr5.4:57827688-57827707 None:intergenic 40.0%
! ACTTGTTTCGAGGCTCATTT+TGG - chr5.4:57826557-57826576 MS.gene26196:intron 40.0%
! AGAATGGGTCCTTTTGTAAC+AGG + chr5.4:57825902-57825921 None:intergenic 40.0%
! CATCTTTTCCTCGTAAGTAG+TGG + chr5.4:57824871-57824890 None:intergenic 40.0%
! CATTTTCTCTTCCTCTTCCT+TGG + chr5.4:57827512-57827531 None:intergenic 40.0%
! CGGTTGGTTATGGTTAGAAA+AGG + chr5.4:57826300-57826319 None:intergenic 40.0%
! GAAATGGAGTACTAGGATCT+AGG + chr5.4:57827099-57827118 None:intergenic 40.0%
! GGACAATTTCCATGGGTTTT+AGG - chr5.4:57823600-57823619 MS.gene26196:intron 40.0%
! GGTCCTTTTGTAACAGGTAT+AGG + chr5.4:57825896-57825915 None:intergenic 40.0%
! TCTTCCTTGTTTGAAGATGC+CGG + chr5.4:57826320-57826339 None:intergenic 40.0%
! TGTGTTGGAAATGGAGTACT+AGG + chr5.4:57827106-57827125 None:intergenic 40.0%
!! AAGCTGACTTGGAAGATAAG+AGG - chr5.4:57826041-57826060 MS.gene26196:intron 40.0%
!! TTGGACGAACTTCTAGCTTA+CGG - chr5.4:57826576-57826595 MS.gene26196:intron 40.0%
!! TTTTTACATTAGCGGTGTCG+AGG - chr5.4:57824455-57824474 MS.gene26196:intron 40.0%
!!! ACTCATTCTGGTTTTGTTGC+AGG - chr5.4:57825983-57826002 MS.gene26196:intron 40.0%
!!! CTCATTCTGGTTTTGTTGCA+GGG - chr5.4:57825984-57826003 MS.gene26196:intron 40.0%
ACGTGTCATACGAGTGTCAT+AGG + chr5.4:57824025-57824044 None:intergenic 45.0%
ACTCCTTGAATAAAGTGGGC+TGG - chr5.4:57826908-57826927 MS.gene26196:intron 45.0%
AGTACATGGGAGCTAAGCTA+TGG + chr5.4:57825140-57825159 None:intergenic 45.0%
CACTTCAGCAATACCAGACT+AGG + chr5.4:57827637-57827656 None:intergenic 45.0%
CATGGTAATCGAGTTTGACC+GGG + chr5.4:57824786-57824805 None:intergenic 45.0%
CCATGGTAATCGAGTTTGAC+CGG + chr5.4:57824787-57824806 None:intergenic 45.0%
CCGGTTAAGAGCGAACTATT+TGG - chr5.4:57824705-57824724 MS.gene26196:intron 45.0%
CGAATCAAAGCAGCAATGCA+TGG + chr5.4:57826955-57826974 None:intergenic 45.0%
CGGTCAAACTCGATTACCAT+GGG - chr5.4:57824785-57824804 MS.gene26196:intron 45.0%
GAACAGCCAAGAAGTTACCA+AGG - chr5.4:57828275-57828294 MS.gene26196:intron 45.0%
GAGTCATGAGCTGGATAAGT+TGG + chr5.4:57827260-57827279 None:intergenic 45.0%
GGGCTGGATTAATTCGTTGT+TGG - chr5.4:57826924-57826943 MS.gene26196:intron 45.0%
GGTCAAACTCGATTACCATG+GGG - chr5.4:57824786-57824805 MS.gene26196:intron 45.0%
GTAACTCATACGAGCTGTGT+TGG - chr5.4:57824127-57824146 MS.gene26196:intron 45.0%
GTCATTTACTGCCAACGTCT+GGG + chr5.4:57825070-57825089 None:intergenic 45.0%
GTCTGGTATTGCTGAAGTGA+AGG - chr5.4:57827638-57827657 MS.gene26196:intron 45.0%
TACTTCGACACTCCTAAGAC+AGG - chr5.4:57824090-57824109 MS.gene26196:intron 45.0%
TAGTAGTGTGGGTGCATCAA+TGG - chr5.4:57824916-57824935 MS.gene26196:intron 45.0%
TGAACAACATGATGCAGCTC+TGG + chr5.4:57828359-57828378 None:intergenic 45.0%
TGCTGGTAACAAAGCTGACT+TGG - chr5.4:57826030-57826049 MS.gene26196:intron 45.0%
! AGCATTTTTCTCCCAGACGT+TGG - chr5.4:57825056-57825075 MS.gene26196:intron 45.0%
! AGTTCTACTATCGGTGCTTC+TGG - chr5.4:57826853-57826872 MS.gene26196:intron 45.0%
! CTAGAGTACCACTACTTACG+AGG - chr5.4:57824860-57824879 MS.gene26196:intron 45.0%
! GTCCCAATCAGGTTGTACAA+AGG + chr5.4:57826375-57826394 None:intergenic 45.0%
! GTGGGTGCATCAATGGTTTT+GGG - chr5.4:57824923-57824942 MS.gene26196:intron 45.0%
! GTTAGGCAGATTCGACTTGA+AGG - chr5.4:57825779-57825798 MS.gene26196:CDS 45.0%
! GTTCTACTATCGGTGCTTCT+GGG - chr5.4:57826854-57826873 MS.gene26196:intron 45.0%
! TACGAGTGTCATAGGAGTGT+CGG + chr5.4:57824017-57824036 None:intergenic 45.0%
! TGTGGGTGCATCAATGGTTT+TGG - chr5.4:57824922-57824941 MS.gene26196:intron 45.0%
! TTGAAGATGCCGGTTGGTTA+TGG + chr5.4:57826310-57826329 None:intergenic 45.0%
! TTGTGCCCTTGGTAACTTCT+TGG + chr5.4:57828284-57828303 None:intergenic 45.0%
! TTTGAGATTTGGGACACAGC+AGG - chr5.4:57825102-57825121 MS.gene26196:intron 45.0%
!! TACCTTGCTTCTGAAGCTCT+TGG + chr5.4:57825451-57825470 None:intergenic 45.0%
!! TTATGCTGCTACGTTTTGGG+TGG - chr5.4:57826231-57826250 MS.gene26196:intron 45.0%
!!! GTCACAAGGGCTTTTTCAGT+TGG + chr5.4:57826631-57826650 None:intergenic 45.0%
!!! TTTTCCTTTCTTTAGGGCCC+TGG - chr5.4:57825296-57825315 MS.gene26196:intron 45.0%
!!! AAAAAAAATCAATTTTTTTT+TGG + chr5.4:57823631-57823650 None:intergenic 5.0%
!!! TTTAAAATTTAATAATATGA+TGG - chr5.4:57823756-57823775 MS.gene26196:intron 5.0%
AGCAGCGCCTCTATAGTACA+TGG + chr5.4:57825154-57825173 None:intergenic 50.0%
ATCCGAGTTTGAATCCTGGC+TGG - chr5.4:57824732-57824751 MS.gene26196:intron 50.0%
CAACAAGAACCATCCCTGCT+GGG + chr5.4:57828318-57828337 None:intergenic 50.0%
CCAACCGGCATCTTCAAACA+AGG - chr5.4:57826313-57826332 MS.gene26196:intron 50.0%
CCGGTCAAACTCGATTACCA+TGG - chr5.4:57824784-57824803 MS.gene26196:intron 50.0%
CGTCATTTACTGCCAACGTC+TGG + chr5.4:57825071-57825090 None:intergenic 50.0%
CTCCAGCCAGGATTCAAACT+CGG + chr5.4:57824737-57824756 None:intergenic 50.0%
GACACTCGTATGACACGTGT+CGG - chr5.4:57824027-57824046 MS.gene26196:intron 50.0%
GCAAAAGCCACGACCATGTT+CGG + chr5.4:57826013-57826032 None:intergenic 50.0%
GCAGCGCCTCTATAGTACAT+GGG + chr5.4:57825153-57825172 None:intergenic 50.0%
GTCCAAGAGCTTCAGAAGCA+AGG - chr5.4:57825446-57825465 MS.gene26196:intron 50.0%
TCAACAAGAACCATCCCTGC+TGG + chr5.4:57828319-57828338 None:intergenic 50.0%
TGGCTAAGAATACTCCAGCC+AGG + chr5.4:57824749-57824768 None:intergenic 50.0%
TGTTGCAGGGAATCCGAACA+TGG - chr5.4:57825997-57826016 MS.gene26196:intron 50.0%
! AACATGGTCGTGGCTTTTGC+TGG - chr5.4:57826013-57826032 MS.gene26196:intron 50.0%
! CCTTGTTTGAAGATGCCGGT+TGG + chr5.4:57826316-57826335 None:intergenic 50.0%
! CTTGTTGATAGACCTGCCGA+AGG - chr5.4:57828331-57828350 MS.gene26196:CDS 50.0%
! GGAAGCACGTGTATATGCTG+AGG - chr5.4:57827292-57827311 MS.gene26196:intron 50.0%
! TGATGCAGCTCTGGTTCCTT+CGG + chr5.4:57828350-57828369 None:intergenic 50.0%
!! GCACAAACACCTCTAGGACT+AGG - chr5.4:57823978-57823997 MS.gene26196:intron 50.0%
AACCAGCGCAAAACCCAGCA+GGG - chr5.4:57828302-57828321 MS.gene26196:intron 55.0%
ACGTGTCGGACAGCTAAGAC+TGG - chr5.4:57824041-57824060 MS.gene26196:intron 55.0%
AGGGAATCCGAACATGGTCG+TGG - chr5.4:57826003-57826022 MS.gene26196:intron 55.0%
ATGAAGCATGGACTCCGACG+CGG + chr5.4:57824484-57824503 None:intergenic 55.0%
TCTTAACCCTCCGGTTCCCA+GGG + chr5.4:57824818-57824837 None:intergenic 55.0%
!! AAGCATGGTCTCTGGAGCCA+GGG + chr5.4:57825316-57825335 None:intergenic 55.0%
!! AGGTGTCGGACACCTGTCTT+AGG + chr5.4:57824105-57824124 None:intergenic 55.0%
!! CTCTAGGACTAGGTGTGTCG+CGG - chr5.4:57823988-57824007 MS.gene26196:intron 55.0%
!!! TTTTGCGCTGGTTGTGCCCT+TGG + chr5.4:57828295-57828314 None:intergenic 55.0%
AGCGCAAAACCCAGCAGGGA+TGG - chr5.4:57828306-57828325 MS.gene26196:intron 60.0%
CAACCAGCGCAAAACCCAGC+AGG - chr5.4:57828301-57828320 MS.gene26196:intron 60.0%
CGCGACACACCTAGTCCTAG+AGG + chr5.4:57823990-57824009 None:intergenic 60.0%
CTGGCTCCAGAGACCATGCT+TGG - chr5.4:57825315-57825334 MS.gene26196:intron 60.0%
CTTAACCCTCCGGTTCCCAG+GGG + chr5.4:57824817-57824836 None:intergenic 60.0%
GGAGCCAGGGCCCTAAAGAA+AGG + chr5.4:57825303-57825322 None:intergenic 60.0%
GTCTTAACCCTCCGGTTCCC+AGG + chr5.4:57824819-57824838 None:intergenic 60.0%
TACCATGGGGCCTTTCCCCT+GGG - chr5.4:57824799-57824818 MS.gene26196:intron 60.0%
TTACCATGGGGCCTTTCCCC+TGG - chr5.4:57824798-57824817 MS.gene26196:intron 60.0%
! ATCCCTGCTGGGTTTTGCGC+TGG + chr5.4:57828307-57828326 None:intergenic 60.0%
!! CAAGCATGGTCTCTGGAGCC+AGG + chr5.4:57825317-57825336 None:intergenic 60.0%
CCTTTCCCCTGGGAACCGGA+GGG - chr5.4:57824809-57824828 MS.gene26196:intron 65.0%
TTCCCAGGGGAAAGGCCCCA+TGG + chr5.4:57824804-57824823 None:intergenic 65.0%
! GCAGCTCTGGTTCCTTCGGC+AGG + chr5.4:57828346-57828365 None:intergenic 65.0%
CCCTCCGGTTCCCAGGGGAA+AGG + chr5.4:57824812-57824831 None:intergenic 70.0%
GCCTTTCCCCTGGGAACCGG+AGG - chr5.4:57824808-57824827 MS.gene26196:intron 70.0%
GGGGCCTTTCCCCTGGGAAC+CGG - chr5.4:57824805-57824824 MS.gene26196:intron 70.0%
! CGGTGTCGAGGTGTCCGCGT+CGG - chr5.4:57824467-57824486 MS.gene26196:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 57823472 57828381 57823472 ID=MS.gene26196
chr5.4 mRNA 57823472 57828381 57823472 ID=MS.gene26196.t1;Parent=MS.gene26196
chr5.4 exon 57828319 57828381 57828319 ID=MS.gene26196.t1.exon1;Parent=MS.gene26196.t1
chr5.4 CDS 57828319 57828381 57828319 ID=cds.MS.gene26196.t1;Parent=MS.gene26196.t1
chr5.4 exon 57826647 57826814 57826647 ID=MS.gene26196.t1.exon2;Parent=MS.gene26196.t1
chr5.4 CDS 57826647 57826814 57826647 ID=cds.MS.gene26196.t1;Parent=MS.gene26196.t1
chr5.4 exon 57826386 57826434 57826386 ID=MS.gene26196.t1.exon3;Parent=MS.gene26196.t1
chr5.4 CDS 57826386 57826434 57826386 ID=cds.MS.gene26196.t1;Parent=MS.gene26196.t1
chr5.4 exon 57825775 57825848 57825775 ID=MS.gene26196.t1.exon4;Parent=MS.gene26196.t1
chr5.4 CDS 57825775 57825848 57825775 ID=cds.MS.gene26196.t1;Parent=MS.gene26196.t1
chr5.4 exon 57824467 57824560 57824467 ID=MS.gene26196.t1.exon5;Parent=MS.gene26196.t1
chr5.4 CDS 57824467 57824560 57824467 ID=cds.MS.gene26196.t1;Parent=MS.gene26196.t1
chr5.4 exon 57823472 57823572 57823472 ID=MS.gene26196.t1.exon6;Parent=MS.gene26196.t1
chr5.4 CDS 57823472 57823572 57823472 ID=cds.MS.gene26196.t1;Parent=MS.gene26196.t1
Gene Sequence

>MS.gene26196

ATGGGTGCTGGAAAATCTAGCCTTGTTTTGCGTTTTGTTAAGGGTCAATTCCTTGAATTTCAGGTTTTTTTTCCTTTTGATTTATCTTTTAAATTCAATTGGGTAATTGTAGAATTCAAAATTTATGAGGACAATTTCCATGGGTTTTAGGTTTAACCAAAAAAAAATTGATTTTTTTTTCAAGTTTGAAAATTGTTTTTTTGTTGTTGCAACTGTTGATTTTGTTGTGTTTTTGACTAAATTGAAATTGGGGTTTTCCTGTTATGATTTTGTAATCGAGTGTTTTTAAAATTTAATAATATGATGGAGAAGATTAGAGAAGTTCTATTCTGAACATGATAGGATTGTTGTGAGAATGAATTGTTATGGTTTTGGAGGTTTGAATGGGATCATTCTTAGATTAGCTTTAGTGCAGAACTCTTAACTTTTTAATTTTGATTATAATATCTTATATTATAGAATGTATGGTGGTACTTGTGGGACACATGTAACTTATGAGCTATGAAGCACAAACACCTCTAGGACTAGGTGTGTCGCGGTGTCCGACACTCCTATGACACTCGTATGACACGTGTCGGACAGCTAAGACTGGTGTCCTAAATAAAATTGTTTCTTCTTTACTTCGACACTCCTAAGACAGGTGTCCGACACCTATGTAACTCATACGAGCTGTGTTGGACAACTTAAATATTTGAAACAATTTTTTCACTATTGCTTCCACACACTGTAAATACAAGAGTTACATATGTGTTGAGAAATGTTGTTCAATTAGGGGGTTGTAGACATTAAAGTTGATTATAACATTTTTAACTTTTTTGACAGTAGATGAATAAATAAAGATCAAATACTATGATATCTTGTTTCATAACTTTTTCTAATGAAATAAATTGCAAAAGTTAGATGCTTATGTATGTATTTTGATTTTCATTTTAGACCGTCTACAAATAAATTAACATATATGTAGCAGTGTCGGTGTCCTATATTTTTTACATTAGCGGTGTCGAGGTGTCCGCGTCGGAGTCCATGCTTCATAGCATGTTATATTTTTTGGGTTGGCTGAATAGATTAGTCATTACTCTCTTCGTACATAAGATACTTTACTTAAAAGTTCAAACTGGGTGTTGCTTTATGAGTTATCAAACATTCATTTGGATGTTGATCTCACAATAAATGTTTAGTAGTTGTTAACAATTCACCTAACCAAGTTATTTGGGTTAAAGTTGTTCATGAACTCCGGTTAAGAGCGAACTATTTGGAAGAATCCGAGTTTGAATCCTGGCTGGAGTATTCTTAGCCAAAAAAAATTTATCTCCCGGTCAAACTCGATTACCATGGGGCCTTTCCCCTGGGAACCGGAGGGTTAAGACAAAAATAAAATAAAAAATCATCTAGAGTACCACTACTTACGAGGAAAAGATGTTTACAAGAGTGATTATTTTGTATATAGTAGTGTGGGTGCATCAATGGTTTTGGGTTCCGAGATCTGTTGTTATTTATTGCCAATTCTTTAAACAGATTGACTTGTGGGCACTGCTTTGCTGAATTTATTTGGTCTATTGTGAAACAGGAATCAACAATAGGGGCAGCATTTTTCTCCCAGACGTTGGCAGTAAATGACGCCACAGTGAAATTTGAGATTTGGGACACAGCAGGACAAGAGAGATACCATAGCTTAGCTCCCATGTACTATAGAGGCGCTGCTGCTGCTATCATTGTCTATGATATTACTAACTTGGTATGATATCTTTTTCTCTGTATATTAAGATTTAAATGAAGAGAATATCAGTTCTGCTAGTTTGCTTCTTTCTTTCAATTTGTGTGTCATTTTCCTTTCTTTAGGGCCCTGGCTCCAGAGACCATGCTTGGAATATTTTAGTGCTTTAATTGTTTATATATAGTTTATTACTGTGGTTTTAACTTTTATATGATTATTTCTTCTGTTCACAGGAGTCATTTACACGAGCGAAGAAATGGGTCCAAGAGCTTCAGAAGCAAGGTATTTTCTGATTGGTTCTGAAGTTTTCTTATTCTTACATTTTTTCTGGAGTGAAGTAAAAATTTTAGATTGTAAAAAGGAAAACTAAACTATTTATTTTGGTTACAAATTTCTGATGCAGTCTATATGTAAAGCAATGTTGACCATATTCTAATATTTCTTCTTAGTTGAAAATAACTTTCGTCGATTCAATGAAACTATAAAGTCATATTTTATACAAAAAAAAAAATATATAATATAGCATAGCACAAACATGGTTGATAATATTAGACATGATGTATTGAGAGTGATTAATATATGAATTGTGAACGGTTAGGCAGATTCGACTTGAAGGTTCTACTTGCAGAAACACCTACTGAACTTTGCGTTAATTTGAAACTAACCCATGATATTTAATGGTTACCGAAATTTTTTAGCTATTGTTTCCTATACCTGTTACAAAAGGACCCATTCTGGTTGTAATTGTACTTCAAAGTTATAGTGGCAGCTGTAGTTGATTCGATCTAAAATAATGAACTCATTCTGGTTTTGTTGCAGGGAATCCGAACATGGTCGTGGCTTTTGCTGGTAACAAAGCTGACTTGGAAGATAAGAGGAAAGTGACAGCTGAAGTAAGTATTACTATTATTGTGTTTTCAGTTTCACTATAGCATCATAGGAATTATAGTTTCTTCAACTACATTGGTAATTAATTGCAACATTATCTCCACACATTTTTTCTGCTAAATTTTCAAATACCAGGGATGGTTTCATTTGATAACTTTTATGCTGCTACGTTTTGGGTGGTATTTGTTGTTAGTCCCAGTCTCTATAGCTTTGTGTATGAAAACCTTTTCTAACCATAACCAACCGGCATCTTCAAACAAGGAAGATTTTGTGCATTTGAGTCGTCAAATACTTGAATCCTTTGTACAACCTGATTGGGACATTTATGTTCTACATATTTTAACTAATCTGAGCATCTTCAAGTTGAGCTTTATAAATTCAATAATTCAATAATTGCTTTTAACTATTTTAACTAACCTATATCTGAGCTTCTTCAAGTTGAGCTTTATAAATTCAATAATTGCTTTTGCATCATGAGAAACTACTTGTTTCGAGGCTCATTTTGGACGAACTTCTAGCTTACGGAAGTTTCTCGATTAATTAAGAATTTAGTTCCAACTGAAAAAGCCCTTGTGACGTAGTAAGTGTATAGGTGGGATTTGTTGGATGAAAGAGCTTCTTAAGAAATTAAGGCGCAGAAGTCGATTTCAACTTATAAGAGAAGCTATTAGTTGGAACACTCAGCTGTTGCTAAATGAGTAAATGATTTCAATCTTTTCTTTTCCTACAACTGCTTATTGAAAAGGTTATTTTAGCTGACTCTAAACAAGCATGTTGATAGTTCTACTATCGGTGCTTCTGGGTTAGACTAACAAATAGTATATAAAATTATAAACTCCTTGAATAAAGTGGGCTGGATTAATTCGTTGTTGGTTTCACCATGCATTGCTGCTTTGATTCGTAAAGTCATGATATTATGTCCAAAATGAAAATCGCATAAGCTTAACTTCGAAATGTTTGTCTACAGTAATTCCAACTTTAGCTTGTACATTGAATAGTTTACTTTGCTAATTGTTCAGTGTCCTAGATCCTAGTACTCCATTTCCAACACAGAAGATTCTTCAAGTATAATCTACTATTAAAATCATTTTTTATTCTACAATAATCATTAATAGCCTATATGTATTTAAACAGTTGCATATTTTATACATATCCGATGTGCCAGTTACATTTATGGGAACTACCAACTTATCCAGCTCATGACTCTTGTACAATGCAGGAAGCACGTGTATATGCTGAGGAAAATGGTCTGTTTTTCATAGAGACGTCTGCGAAAACTGCAGCCAATGTTAATGACATATTTTATGAAATAGGTGCAAATCCTTTCCTATTACATACACTTTAGGTTGCTTCACAAGTCGTTTATTTTATTTTTACTCTTTAATTTAATTGCATAAGAATAAATCATACAAGGAAGAACATAATTTATATCAGTCCAAGGAAGAGGAAGAGAAAATGGAAACTCTTCTTTTGAGGAAATGCCAAAATATTTGTGTTGTTTGCACTACTGGTTAGATAAATGTACATAAAGGAAATGTGAACAAAATGAATTTTATTATTCCTAGTCTGGTATTGCTGAAGTGAAGGTTCTATTTCAAATGTGCAGCTGAGCCTTTTGATTTGCACCAGACGAATGTGTTTTTTTATGTTTTCTCAACATATGTAGTTTTTTTTAGTCTCTTCGTATAAGACTGAAGAAGGGTATGAATGACATTTGACCATAAAACGTTCATGAGTGAACAGTGCAGTTGATGAATTTCTTAGATATGTGACTTTTTGAACATTGTACGTTTATTGTGAGCATTTCATATAATTACAAGTTTTGTCACTCTGTAGGGTTTTTACAATAGAGCAGACCGTCTCACTAAACAGAATGAGAGAACTATACTGTTTTATTTTGTGTCTATATTCTCTTGCTTGTGCTTTTTAATTGTGTTTCATAATTTTATGTCTGTAAACTTGAACTTGTATACTAATGAAATAATAGTCTTAAGTAAGAGAAAATGGATGAGAAAGAAATTGTACTCTCAATATAAGATGGTATTTTCAAATTTGGAATGATATTTAAGTGTAATAATGGCATACATAAAAAATTCATTCACACAAATAATGGTTTCTCTATGATCCTCTCTCGATGTATTTAAAAAATATTGTGAAGATATGCTGAGATATATTCTAACATGACTTGCAATTGCTTGCGTGAACAGCCAAGAAGTTACCAAGGGCACAACCAGCGCAAAACCCAGCAGGGATGGTTCTTGTTGATAGACCTGCCGAAGGAACCAGAGCTGCATCATGTTGTTCATAA
Protein sequence

>MS.gene26196.t1

MGAGKSSLVLRFVKGQFLEFQESTIGAAFFSQTLAVNDATVKFEIWDTAGQERYHSLAPMYYRGAAAAIIVYDITNLESFTRAKKWVQELQKQGNPNMVVAFAGNKADLEDKRKVTAEEARVYAEENGLFFIETSAKTAANVNDIFYEIAKKLPRAQPAQNPAGMVLVDRPAEGTRAASCCS