Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27927.t1 | XP_003629947.1 | 99.5 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 2.00E-259 | 904.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27927.t1 | Q96254 | 85.8 | 444 | 63 | 0 | 1 | 444 | 1 | 444 | 6.0e-228 | 791.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27927.t1 | G7L8T3 | 99.5 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 1.5e-259 | 904.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene27927 | MS.gene063577 | PPI |
| MS.gene27927 | MS.gene47883 | PPI |
| MS.gene27927 | MS.gene061580 | PPI |
| MS.gene037879 | MS.gene27927 | PPI |
| MS.gene27927 | MS.gene073174 | PPI |
| MS.gene055394 | MS.gene27927 | PPI |
| MS.gene47882 | MS.gene27927 | PPI |
| MS.gene27927 | MS.gene017310 | PPI |
| MS.gene27927 | MS.gene48050 | PPI |
| MS.gene27927 | MS.gene26196 | PPI |
| MS.gene27927 | MS.gene065419 | PPI |
| MS.gene27927 | MS.gene009580 | PPI |
| MS.gene05029 | MS.gene27927 | PPI |
| MS.gene69853 | MS.gene27927 | PPI |
| MS.gene033585 | MS.gene27927 | PPI |
| MS.gene55072 | MS.gene27927 | PPI |
| MS.gene27927 | MS.gene75479 | PPI |
| MS.gene27927 | MS.gene008351 | PPI |
| MS.gene27927 | MS.gene024151 | PPI |
| MS.gene27927 | MS.gene79211 | PPI |
| MS.gene27927 | MS.gene049680 | PPI |
| MS.gene99137 | MS.gene27927 | PPI |
| MS.gene030951 | MS.gene27927 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27927.t1 | MTR_8g088620 | 99.550 | 444 | 2 | 0 | 1 | 444 | 1 | 444 | 0.0 | 917 |
| MS.gene27927.t1 | MTR_4g093980 | 81.193 | 436 | 82 | 0 | 1 | 436 | 1 | 436 | 0.0 | 750 |
| MS.gene27927.t1 | MTR_5g024150 | 25.587 | 426 | 235 | 13 | 5 | 356 | 18 | 435 | 6.46e-27 | 113 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27927.t1 | AT2G44100 | 85.811 | 444 | 63 | 0 | 1 | 444 | 1 | 444 | 0.0 | 803 |
| MS.gene27927.t1 | AT3G59920 | 85.360 | 444 | 65 | 0 | 1 | 444 | 1 | 444 | 0.0 | 798 |
| MS.gene27927.t1 | AT2G44100 | 85.814 | 430 | 61 | 0 | 1 | 430 | 1 | 430 | 0.0 | 781 |
| MS.gene27927.t1 | AT5G09550 | 79.505 | 444 | 91 | 0 | 1 | 444 | 1 | 444 | 0.0 | 754 |
| MS.gene27927.t1 | AT3G06540 | 24.661 | 442 | 250 | 15 | 5 | 366 | 15 | 453 | 1.79e-20 | 94.4 |
Find 110 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGACCCATGACAGAAAATT+TGG | 0.184972 | 4.4:+12409996 | MS.gene27927:intron |
| CACTTCCCTTAATCTTATTC+AGG | 0.214984 | 4.4:+12408546 | MS.gene27927:CDS |
| TCTTTACCTGAATAAGATTA+AGG | 0.215427 | 4.4:-12408552 | None:intergenic |
| GATGATCAAGGAAAGGTTAT+TGG | 0.226106 | 4.4:+12411701 | MS.gene27927:CDS |
| GGCTTTGAAGTCCCCACTTA+TGG | 0.233527 | 4.4:+12409381 | MS.gene27927:CDS |
| TTTGGGTGTTTAGTTCATTA+TGG | 0.255862 | 4.4:+12409116 | MS.gene27927:intron |
| GCACGGCTTAGCGCTGTTTA+TGG | 0.259796 | 4.4:+12410463 | MS.gene27927:CDS |
| TTCAGCTCTGGAAGAAATTC+AGG | 0.276301 | 4.4:+12408661 | MS.gene27927:intron |
| TGACAAGCCTCCTCCTCATT+TGG | 0.302661 | 4.4:+12408689 | MS.gene27927:CDS |
| AGTATATCTACCTTGCATTC+AGG | 0.307677 | 4.4:-12410508 | None:intergenic |
| AACATGTATACGTTGATAAC+TGG | 0.312028 | 4.4:+12414706 | MS.gene27927:CDS |
| CCAGGTTTCAGTTCAATTGC+AGG | 0.318540 | 4.4:-12414458 | None:intergenic |
| AGAATACGATGTAATCGTTC+TGG | 0.319703 | 4.4:+12408337 | MS.gene27927:CDS |
| AAACCTGGAATCGACCTTCT+AGG | 0.328334 | 4.4:+12414473 | MS.gene27927:CDS |
| ATATATCCTTTGTATGGTTT+AGG | 0.331062 | 4.4:+12410303 | MS.gene27927:CDS |
| GACAAGCCTCCTCCTCATTT+GGG | 0.351742 | 4.4:+12408690 | MS.gene27927:CDS |
| GGACTTCATTGGTCATGCTT+TGG | 0.364793 | 4.4:+12410037 | MS.gene27927:CDS |
| GATTGGGAGTCATTGGTGTT+GGG | 0.368885 | 4.4:-12412106 | None:intergenic |
| CTTTACCTGAATAAGATTAA+GGG | 0.386834 | 4.4:-12408551 | None:intergenic |
| TCTAGGGTGGGTTCATTGAC+TGG | 0.390022 | 4.4:-12414539 | None:intergenic |
| TATTCTCCCACAAAAGCAAC+TGG | 0.403427 | 4.4:+12412138 | MS.gene27927:CDS |
| GCTTTGAAGTCCCCACTTAT+GGG | 0.406357 | 4.4:+12409382 | MS.gene27927:CDS |
| GAGTCTCTTGCACGATTTCA+AGG | 0.410458 | 4.4:+12410267 | MS.gene27927:CDS |
| CCTTACATATATCCTTTGTA+TGG | 0.411629 | 4.4:+12410297 | MS.gene27927:CDS |
| GGTCCTAGAAGGTCGATTCC+AGG | 0.413023 | 4.4:-12414476 | None:intergenic |
| TCTTTACTCTTCTGCAGCAC+TGG | 0.413041 | 4.4:-12415332 | None:intergenic |
| TGACTTACATGTCTGACTTT+CGG | 0.427909 | 4.4:-12412162 | None:intergenic |
| CGATTGGGAGTCATTGGTGT+TGG | 0.428832 | 4.4:-12412107 | None:intergenic |
| ACCTTGTTGGTCAAGTATGA+TGG | 0.428877 | 4.4:-12411773 | None:intergenic |
| CTGAATAAGATTAAGGGAAG+TGG | 0.439368 | 4.4:-12408545 | None:intergenic |
| AAAGCCTCCATGTCATTTGA+AGG | 0.440668 | 4.4:-12409364 | None:intergenic |
| TCTCACAATGTTTGCCCAAA+GGG | 0.442192 | 4.4:+12414392 | MS.gene27927:CDS |
| TTGCACCGATTGGGAGTCAT+TGG | 0.442455 | 4.4:-12412113 | None:intergenic |
| TCGAAATAAAGCAGTTGTCT+AGG | 0.450436 | 4.4:-12414556 | None:intergenic |
| CCTGCAATTGAACTGAAACC+TGG | 0.453263 | 4.4:+12414458 | MS.gene27927:CDS |
| GAGTCATTGGTGTTGGGGAT+CGG | 0.454805 | 4.4:-12412100 | None:intergenic |
| CTCTTCACAGTGTCTAGTGC+TGG | 0.455178 | 4.4:-12410092 | None:intergenic |
| GATAACACTGTGGACTTCAT+TGG | 0.464458 | 4.4:+12410026 | MS.gene27927:CDS |
| GAGAATAACTTGCACCGATT+GGG | 0.465079 | 4.4:-12412122 | None:intergenic |
| GATGTAATCGTTCTGGGAAC+TGG | 0.470059 | 4.4:+12408344 | MS.gene27927:CDS |
| ATGGATAGAAATGATTATTA+CGG | 0.478912 | 4.4:+12408514 | MS.gene27927:CDS |
| TCTCATCAACAGGTCCTAGA+AGG | 0.482856 | 4.4:-12414487 | None:intergenic |
| TTTCAAATATTCCCATAAGT+GGG | 0.483406 | 4.4:-12409393 | None:intergenic |
| GGAGAATAACTTGCACCGAT+TGG | 0.484322 | 4.4:-12412123 | None:intergenic |
| GAATACGATGTAATCGTTCT+GGG | 0.496229 | 4.4:+12408338 | MS.gene27927:CDS |
| CGAAATAAAGCAGTTGTCTA+GGG | 0.502383 | 4.4:-12414555 | None:intergenic |
| CTCTATTTCAAAGCTGTGGA+TGG | 0.502434 | 4.4:+12409189 | MS.gene27927:CDS |
| TCACTTTGAATCAACAGTAC+TGG | 0.502679 | 4.4:+12414675 | MS.gene27927:CDS |
| GTTATTGGCGTCACGTCTGA+AGG | 0.507245 | 4.4:+12411716 | MS.gene27927:CDS |
| GTTTAGTTCATTATGGCCAA+TGG | 0.508001 | 4.4:+12409123 | MS.gene27927:intron |
| CAGGTGGAATTTGATGATCA+AGG | 0.509665 | 4.4:+12411689 | MS.gene27927:intron |
| ACTGATCCTAAAACTCATGA+TGG | 0.512382 | 4.4:+12409463 | MS.gene27927:CDS |
| CCATACAAAGGATATATGTA+AGG | 0.512388 | 4.4:-12410297 | None:intergenic |
| AGTGGTCTTCTCTCCGTCGA+TGG | 0.517094 | 4.4:+12408386 | MS.gene27927:CDS |
| AACCCTCACTAATGTGCCAT+TGG | 0.520084 | 4.4:-12409139 | None:intergenic |
| ACCATTTCAACAGGTGAGGA+AGG | 0.530785 | 4.4:+12412039 | MS.gene27927:intron |
| TTTCAACAGGTGAGGAAGGT+TGG | 0.530894 | 4.4:+12412043 | MS.gene27927:intron |
| CTAGAACCCAAATGAGGAGG+AGG | 0.539538 | 4.4:-12408696 | None:intergenic |
| TCCTAAAACTCATGATGGGA+TGG | 0.544577 | 4.4:+12409468 | MS.gene27927:CDS |
| ACAGGTGAGGAAGGTTGGTA+AGG | 0.545562 | 4.4:+12412048 | MS.gene27927:intron |
| TTCTCACAATGTTTGCCCAA+AGG | 0.554054 | 4.4:+12414391 | MS.gene27927:CDS |
| TGGTTTAGGAGAGCTTCCTC+AGG | 0.555597 | 4.4:+12410317 | MS.gene27927:CDS |
| GTTGAATAAACCTGAATGCA+AGG | 0.558302 | 4.4:+12410498 | MS.gene27927:CDS |
| ATTGGGAGTCATTGGTGTTG+GGG | 0.558324 | 4.4:-12412105 | None:intergenic |
| GAGAATACGAGCAACAGAAT+AGG | 0.558919 | 4.4:-12414373 | None:intergenic |
| TATTGGTACAGGCATTTGCA+CGG | 0.559199 | 4.4:+12410446 | MS.gene27927:intron |
| CATTGGTGTTGGGGATCGGA+TGG | 0.560062 | 4.4:-12412096 | None:intergenic |
| CAAGGTACCTTCAAATGACA+TGG | 0.560179 | 4.4:+12409357 | MS.gene27927:CDS |
| GGAAGGTTGGTAAGGTTGCA+AGG | 0.561162 | 4.4:+12412056 | MS.gene27927:CDS |
| AGTCTCTTGCACGATTTCAA+GGG | 0.561340 | 4.4:+12410268 | MS.gene27927:CDS |
| CGGCTTAGCGCTGTTTATGG+AGG | 0.561599 | 4.4:+12410466 | MS.gene27927:CDS |
| GGAATTTGATGATCAAGGAA+AGG | 0.566365 | 4.4:+12411694 | MS.gene27927:CDS |
| TTCAAATATTCCCATAAGTG+GGG | 0.568438 | 4.4:-12409392 | None:intergenic |
| CTTGACAAGAGTCACTACTA+AGG | 0.569477 | 4.4:+12409492 | MS.gene27927:CDS |
| CTTTGTCTTTAACAAAGCAA+AGG | 0.569984 | 4.4:+12409215 | MS.gene27927:CDS |
| TGGCCAATGGCACATTAGTG+AGG | 0.571838 | 4.4:+12409136 | MS.gene27927:CDS |
| AATAAAGCAGTTGTCTAGGG+TGG | 0.573026 | 4.4:-12414552 | None:intergenic |
| TCAGCTCTGGAAGAAATTCA+GGG | 0.573618 | 4.4:+12408662 | MS.gene27927:intron |
| GTATCTCTATTTCAAAGCTG+TGG | 0.574401 | 4.4:+12409185 | MS.gene27927:CDS |
| GTCTCTTGCACGATTTCAAG+GGG | 0.577654 | 4.4:+12410269 | MS.gene27927:CDS |
| CAACACCAATGACTCCCAAT+CGG | 0.583889 | 4.4:+12412108 | MS.gene27927:CDS |
| AGTCCACAGTGTTATCATCA+AGG | 0.587304 | 4.4:-12410019 | None:intergenic |
| GGCCAATGGCACATTAGTGA+GGG | 0.588085 | 4.4:+12409137 | MS.gene27927:CDS |
| GGTTACTTTGAGGCCATCGA+CGG | 0.588114 | 4.4:-12408399 | None:intergenic |
| CTGATCCTAAAACTCATGAT+GGG | 0.591782 | 4.4:+12409464 | MS.gene27927:CDS |
| TAATATTGATATGAACCCTA+AGG | 0.595316 | 4.4:+12408728 | MS.gene27927:CDS |
| TACTGTTGATTCAAAGTGAG+TGG | 0.602582 | 4.4:-12414671 | None:intergenic |
| GAAGGTTGGTAAGGTTGCAA+GGG | 0.604709 | 4.4:+12412057 | MS.gene27927:CDS |
| ATAAAGCAGTTGTCTAGGGT+GGG | 0.607677 | 4.4:-12414551 | None:intergenic |
| TTATTGGCGTCACGTCTGAA+GGG | 0.608673 | 4.4:+12411717 | MS.gene27927:CDS |
| GGGGATCGGATGGCTCATGA+TGG | 0.613814 | 4.4:-12412086 | None:intergenic |
| GTATACGTTGATAACTGGAA+AGG | 0.621367 | 4.4:+12414711 | MS.gene27927:CDS |
| GATAGAAATGATTATTACGG+AGG | 0.624916 | 4.4:+12408517 | MS.gene27927:CDS |
| TTATCAACGTATACATGTTG+AGG | 0.629009 | 4.4:-12414702 | None:intergenic |
| TATTGGCGTCACGTCTGAAG+GGG | 0.631609 | 4.4:+12411718 | MS.gene27927:CDS |
| TTACTCTTCTGCAGCACTGG+CGG | 0.636142 | 4.4:-12415329 | None:intergenic |
| AGACACTGTGAAGAGAATGA+AGG | 0.644888 | 4.4:+12410100 | MS.gene27927:CDS |
| TCTTTGCTAGAACCCAAATG+AGG | 0.649689 | 4.4:-12408702 | None:intergenic |
| TATTGCATTTGTATCAACTG+AGG | 0.650673 | 4.4:+12414421 | MS.gene27927:CDS |
| AGCTCTCCTAAACCATACAA+AGG | 0.658728 | 4.4:-12410309 | None:intergenic |
| TTGCTAGAACCCAAATGAGG+AGG | 0.659828 | 4.4:-12408699 | None:intergenic |
| ATTCTCCCACAAAAGCAACT+GGG | 0.662559 | 4.4:+12412139 | MS.gene27927:CDS |
| TCCATCATACTTGACCAACA+AGG | 0.680703 | 4.4:+12411772 | MS.gene27927:CDS |
| CTCAAAGAATGCATTCTCAG+TGG | 0.682881 | 4.4:+12408368 | MS.gene27927:CDS |
| CAGCTCTGGAAGAAATTCAG+GGG | 0.685860 | 4.4:+12408663 | MS.gene27927:intron |
| GGTACCTTCAAATGACATGG+AGG | 0.738325 | 4.4:+12409360 | MS.gene27927:CDS |
| CGGCACTGAGATCCACAGTG+AGG | 0.743753 | 4.4:-12415309 | None:intergenic |
| TCTCTTGCACGATTTCAAGG+GGG | 0.764721 | 4.4:+12410270 | MS.gene27927:CDS |
| TGGCCTTGATGATAACACTG+TGG | 0.769317 | 4.4:+12410016 | MS.gene27927:CDS |
| ACAGGTTCTAGACCTCACTG+TGG | 0.826301 | 4.4:+12415297 | MS.gene27927:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 12408329 | 12415351 | 12408329 | ID=MS.gene27927 |
| chr4.4 | mRNA | 12408329 | 12415351 | 12408329 | ID=MS.gene27927.t1;Parent=MS.gene27927 |
| chr4.4 | exon | 12408329 | 12408415 | 12408329 | ID=MS.gene27927.t1.exon1;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12408329 | 12408415 | 12408329 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12408505 | 12408567 | 12408505 | ID=MS.gene27927.t1.exon2;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12408505 | 12408567 | 12408505 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12408666 | 12408749 | 12408666 | ID=MS.gene27927.t1.exon3;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12408666 | 12408749 | 12408666 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12409129 | 12409236 | 12409129 | ID=MS.gene27927.t1.exon4;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12409129 | 12409236 | 12409129 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12409352 | 12409524 | 12409352 | ID=MS.gene27927.t1.exon5;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12409352 | 12409524 | 12409352 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12410010 | 12410121 | 12410010 | ID=MS.gene27927.t1.exon6;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12410010 | 12410121 | 12410010 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12410258 | 12410338 | 12410258 | ID=MS.gene27927.t1.exon7;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12410258 | 12410338 | 12410258 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12410457 | 12410519 | 12410457 | ID=MS.gene27927.t1.exon8;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12410457 | 12410519 | 12410457 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12411692 | 12411793 | 12411692 | ID=MS.gene27927.t1.exon9;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12411692 | 12411793 | 12411692 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12412052 | 12412176 | 12412052 | ID=MS.gene27927.t1.exon10;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12412052 | 12412176 | 12412052 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12414370 | 12414580 | 12414370 | ID=MS.gene27927.t1.exon11;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12414370 | 12414580 | 12414370 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12414658 | 12414732 | 12414658 | ID=MS.gene27927.t1.exon12;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12414658 | 12414732 | 12414658 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| chr4.4 | exon | 12415301 | 12415351 | 12415301 | ID=MS.gene27927.t1.exon13;Parent=MS.gene27927.t1 |
| chr4.4 | CDS | 12415301 | 12415351 | 12415301 | ID=cds.MS.gene27927.t1;Parent=MS.gene27927.t1 |
| Gene Sequence |
| Protein sequence |
