AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033069

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033069.t1 MTR_4g109450 99.561 912 4 0 1 912 1 912 0.0 1830
MS.gene033069.t1 MTR_4g109450 99.632 816 3 0 1 816 1 816 0.0 1639
MS.gene033069.t1 MTR_7g028970 87.390 912 109 2 1 912 1 906 0.0 1600
MS.gene033069.t1 MTR_7g012820 51.438 869 394 9 1 853 83 939 0.0 871
MS.gene033069.t1 MTR_7g012820 51.438 869 394 9 1 853 12 868 0.0 871
MS.gene033069.t1 MTR_6g013660 51.557 867 396 8 1 853 79 935 0.0 866
MS.gene033069.t1 MTR_3g106160 49.107 896 426 12 5 883 95 977 0.0 822
MS.gene033069.t1 MTR_2g099075 71.698 477 125 3 400 875 4 471 0.0 670
MS.gene033069.t1 MTR_8g100040 45.478 774 334 11 131 886 222 925 0.0 652
MS.gene033069.t1 MTR_3g102830 46.205 751 325 10 126 858 218 907 0.0 644
MS.gene033069.t1 MTR_3g498725 46.515 746 320 12 131 858 218 902 0.0 644
MS.gene033069.t1 MTR_3g098310 43.855 716 324 12 162 860 296 950 0.0 563
MS.gene033069.t1 MTR_2g006950 68.095 420 94 7 461 878 1 382 0.0 544
MS.gene033069.t1 MTR_2g006950 71.371 248 51 3 657 901 365 595 1.05e-106 342
MS.gene033069.t1 MTR_3g098310 45.389 553 235 10 162 707 296 788 7.13e-145 449
MS.gene033069.t1 MTR_3g461000 45.697 337 178 3 534 867 427 761 2.27e-101 335
MS.gene033069.t1 MTR_3g461000 35.109 413 253 7 4 404 3 412 7.47e-67 239
MS.gene033069.t1 MTR_7g096480 72.674 172 23 2 716 887 96 243 5.77e-76 248
MS.gene033069.t1 MTR_7g096480 53.906 128 26 1 530 657 1 95 5.76e-33 128
MS.gene033069.t1 MTR_6g048860 48.810 168 83 2 127 292 99 265 6.43e-45 166
MS.gene033069.t1 MTR_5g098360 77.895 95 20 1 558 652 74 167 5.63e-41 148
MS.gene033069.t1 MTR_1614s0010 41.709 199 109 3 638 835 1 193 2.77e-31 121
MS.gene033069.t1 MTR_1g076440 61.628 86 33 0 631 716 158 243 5.20e-30 121
MS.gene033069.t1 MTR_1g057150 28.239 301 182 11 20 292 26 320 9.25e-22 101
MS.gene033069.t1 MTR_1g090640 27.273 297 181 11 17 285 24 313 3.48e-17 87.0
MS.gene033069.t1 MTR_4g129230 31.364 220 108 8 26 208 32 245 2.06e-14 78.2
MS.gene033069.t1 MTR_2g038200 29.845 258 144 9 20 241 26 282 1.01e-13 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033069.t1 AT1G74310 86.585 902 114 4 1 901 1 896 0.0 1602
MS.gene033069.t1 AT1G74310 87.868 816 96 1 1 816 1 813 0.0 1478
MS.gene033069.t1 AT5G15450 50.579 864 408 8 1 853 77 932 0.0 858
MS.gene033069.t1 AT4G14670 66.970 660 176 5 1 658 1 620 0.0 850
MS.gene033069.t1 AT2G25140 49.542 874 415 8 1 859 83 945 0.0 838
MS.gene033069.t1 AT5G50920 46.577 745 320 12 131 858 221 904 0.0 644
MS.gene033069.t1 AT3G48870 45.845 746 325 9 131 858 210 894 0.0 632
MS.gene033069.t1 AT3G48870 45.845 746 325 9 131 858 241 925 0.0 632
MS.gene033069.t1 AT3G48870 45.845 746 325 9 131 858 241 925 0.0 632
MS.gene033069.t1 AT3G48870 45.845 746 325 9 131 858 241 925 0.0 632
MS.gene033069.t1 AT5G51070 38.846 901 434 19 4 851 81 917 0.0 559
MS.gene033069.t1 AT4G14675 49.324 148 43 1 720 867 1 116 5.05e-42 151
MS.gene033069.t1 AT3G45450 47.727 176 74 3 176 350 151 309 6.63e-41 154
MS.gene033069.t1 AT3G52490 31.399 293 163 12 20 293 26 299 8.84e-23 105
MS.gene033069.t1 AT5G57710 30.213 235 134 10 26 234 32 262 3.09e-15 80.9
MS.gene033069.t1 AT1G07200 26.875 160 108 3 570 728 68 219 3.37e-11 67.0

Find 209 sgRNAs with CRISPR-Local

Find 252 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACCTTTCTTTCTCATTT+TGG 0.103964 8.2:-16363847 None:intergenic
CATCTCTTGAGTGGACTTTC+AGG 0.168367 8.2:+16364497 MS.gene033069:CDS
AAGAACAATCCTGTTCTTAT+TGG 0.183649 8.2:+16362280 MS.gene033069:CDS
TTTCAAGCATTGAAGACTTA+TGG 0.255904 8.2:+16362163 MS.gene033069:CDS
GGGAAGCTTGATCCTGTTAT+TGG 0.264082 8.2:+16362208 MS.gene033069:CDS
TTCTTATTCCTTGGTCCAAC+TGG 0.268223 8.2:+16363992 MS.gene033069:CDS
CTCTCTAGCCACCTTCTAAT+TGG 0.271216 8.2:-16364891 None:intergenic
ATGAGATTCATCTTGTTCTT+GGG 0.276920 8.2:+16362518 MS.gene033069:CDS
TCCAGTCGGTTCAACAATTC+TGG 0.285531 8.2:-16364581 None:intergenic
GATGAGATTCATCTTGTTCT+TGG 0.287438 8.2:+16362517 MS.gene033069:CDS
AAGCCGGTGTTGCTGTTTCT+AGG 0.291735 8.2:+16362068 MS.gene033069:CDS
GGCTAACAACCTCGGCTATT+TGG 0.297052 8.2:-16363793 None:intergenic
TAGGTTAATTGCTTTGGATA+TGG 0.306154 8.2:+16362390 MS.gene033069:CDS
TCTTCGTCGCGTCCAATAAC+AGG 0.320228 8.2:-16362220 None:intergenic
CAAGCAATTTCAAACGTTGC+TGG 0.347559 8.2:+16361824 MS.gene033069:CDS
AGCTGTTGATCAGTTAATCT+TGG 0.350267 8.2:+16362006 MS.gene033069:CDS
GGGATTGTTAATGCTGAAAC+TGG 0.353298 8.2:+16365035 MS.gene033069:CDS
AAGGCAGAACTGTGGACTTT+AGG 0.354025 8.2:+16364435 MS.gene033069:CDS
TTGTTTCTTGTGTAGGTGTA+TGG 0.362917 8.2:+16364861 MS.gene033069:intron
TGGTTGCTGGTGCGAAGTAT+AGG 0.370808 8.2:+16362419 MS.gene033069:CDS
AGGAAGTAAGAAGGCATTTC+AGG 0.374785 8.2:+16364558 MS.gene033069:CDS
ACTGGTTCCTTCTTATTCCT+TGG 0.375288 8.2:+16363983 MS.gene033069:CDS
TTGAGATCAAGAGCTGGTTT+AGG 0.379830 8.2:+16363944 MS.gene033069:CDS
AGGTTAATTGCTTTGGATAT+GGG 0.380520 8.2:+16362391 MS.gene033069:CDS
AGAGCTGCGGACCTGCGATA+TGG 0.381156 8.2:+16363671 MS.gene033069:CDS
ATATCGCAGGTCCGCAGCTC+TGG 0.381186 8.2:-16363669 None:intergenic
GAGGGGTAAAGATGGGAAAA+AGG 0.388224 8.2:+16362114 MS.gene033069:CDS
GATATGGGAGCACTGGTTGC+TGG 0.390282 8.2:+16362406 MS.gene033069:CDS
CTAGCAATCTTGCTGATGTT+AGG 0.395088 8.2:+16362371 MS.gene033069:CDS
CTCAATTGCTCATGTGATAG+AGG 0.400181 8.2:-16364623 None:intergenic
GGTTGCTGGTGCGAAGTATA+GGG 0.400576 8.2:+16362420 MS.gene033069:CDS
TGATGTTAGGTTAATTGCTT+TGG 0.410605 8.2:+16362384 MS.gene033069:CDS
ACCGAAAAGTGATGCTGTTC+AGG 0.411960 8.2:+16365094 MS.gene033069:CDS
CCTGTTCTTATTGGTGAACC+TGG 0.418659 8.2:+16362289 MS.gene033069:CDS
GCTGTTGATCAGTTAATCTT+GGG 0.422333 8.2:+16362007 MS.gene033069:CDS
TAGAATTGATGCAAGGTGTA+AGG 0.422834 8.2:-16361774 None:intergenic
GTGCTACCAGGCACTTCATC+TGG 0.425997 8.2:-16361920 None:intergenic
AAGATGCAGCATTTGAAAGA+AGG 0.427986 8.2:+16362659 MS.gene033069:CDS
TGTGGCTGAACCTAGTGTTC+CGG 0.430850 8.2:+16362696 MS.gene033069:CDS
CAGCAACGTTTGAAATTGCT+TGG 0.431831 8.2:-16361823 None:intergenic
CACTCTGTATCAAGATTGAT+TGG 0.432459 8.2:+16364112 MS.gene033069:CDS
TTCCTTGGTCCAACTGGTGT+TGG 0.432663 8.2:+16363998 MS.gene033069:CDS
CGTGGACAGCTTCGATGTAT+TGG 0.435873 8.2:+16362598 MS.gene033069:CDS
TCAAGATTGTCAATTTCCTC+AGG 0.437119 8.2:-16363030 None:intergenic
TGCTTTGGATATGGGAGCAC+TGG 0.439570 8.2:+16362399 MS.gene033069:CDS
ATTTGAAAGAAGGTTTCAAC+AGG 0.441868 8.2:+16362669 MS.gene033069:CDS
ATTCATCTTGTTCTTGGGGC+TGG 0.444791 8.2:+16362523 MS.gene033069:CDS
GTCAATTAACAGAAGCTGTA+AGG 0.446297 8.2:+16364306 MS.gene033069:CDS
ATGCTCAGCACCAAGGTTAG+AGG 0.449435 8.2:-16364477 None:intergenic
TTACATTGATGCTGGTCCTA+AGG 0.450753 8.2:+16364977 MS.gene033069:CDS
CTTCTACAAGTCTTGGATGA+TGG 0.453206 8.2:+16364395 MS.gene033069:CDS
AAAACAAACGAAGCACTTGC+TGG 0.455382 8.2:+16361707 MS.gene033069:CDS
ACTAAATCAATCGCCTTGTC+AGG 0.457433 8.2:-16362964 None:intergenic
GGAAGTTGATGATGATGAAA+TGG 0.457919 8.2:+16365139 MS.gene033069:CDS
CAACACTCTTCTACAAGTCT+TGG 0.460461 8.2:+16364388 MS.gene033069:CDS
AAGGTTTCAACAGGTTTATG+TGG 0.465704 8.2:+16362678 MS.gene033069:CDS
TGCTCGCGATCGAGTGATGC+AGG 0.466652 8.2:+16364538 MS.gene033069:CDS
CACGAGCTAGCAATGACTTC+AGG 0.466831 8.2:+16361734 MS.gene033069:CDS
CTACAACGCTTGAGGAGTAT+AGG 0.468012 8.2:+16362623 MS.gene033069:CDS
ATTCTCGAGGATTCTCAGAT+TGG 0.468883 8.2:+16362031 MS.gene033069:CDS
AGAGCTCCTCTTTGCACTAC+AGG 0.471282 8.2:+16363622 MS.gene033069:CDS
TTGTCTAGTCGGTACATAAC+CGG 0.472293 8.2:+16362811 MS.gene033069:CDS
AATCTTGAAGGTAGCACTGA+TGG 0.474434 8.2:+16363725 MS.gene033069:CDS
AATTGACATGTCGGAGTACA+TGG 0.475392 8.2:+16364084 MS.gene033069:CDS
AGGTATGTTGGACATGAAGA+AGG 0.476815 8.2:+16364281 MS.gene033069:intron
AAAGAAAGGTTGGTTGGACT+TGG 0.479433 8.2:+16363857 MS.gene033069:CDS
TTGGCTCAGAGGATTGTTAG+AGG 0.480511 8.2:+16362340 MS.gene033069:CDS
ATTCTGATTCAGATTCCAAA+TGG 0.480850 8.2:+16365071 MS.gene033069:CDS
CGAAGAATGCTAATTGTATC+CGG 0.481651 8.2:-16362715 None:intergenic
TTGATAAGCGCGGTGCTACC+AGG 0.482529 8.2:-16361932 None:intergenic
CCAGGTTCACCAATAAGAAC+AGG 0.484297 8.2:-16362289 None:intergenic
TAGAAGGTGGCTAGAGAGGA+AGG 0.489310 8.2:+16364896 MS.gene033069:CDS
TTCTACAAGTCTTGGATGAT+GGG 0.491958 8.2:+16364396 MS.gene033069:CDS
GGTGATTTGTTTAAAGAAGC+CGG 0.497778 8.2:+16362052 MS.gene033069:CDS
CAATTAGAAGGTGGCTAGAG+AGG 0.498681 8.2:+16364892 MS.gene033069:CDS
GGAAGACCTCAACAACCAAC+TGG 0.500438 8.2:+16363965 MS.gene033069:CDS
AGAGACTTGGTTGAACAAGC+AGG 0.500744 8.2:+16362187 MS.gene033069:CDS
AGTATAGTAAAGAGAAAGAG+AGG 0.501091 8.2:+16363564 MS.gene033069:CDS
AATGAGAAAGAAAGGTTGGT+TGG 0.503006 8.2:+16363851 MS.gene033069:CDS
AGATTGATTGGTGCACCACC+AGG 0.503361 8.2:+16364124 MS.gene033069:CDS
GATACAATTAGCATTCTTCG+TGG 0.503772 8.2:+16362718 MS.gene033069:CDS
AGGTTGTGACAGAGCTGTCT+AGG 0.503837 8.2:+16364916 MS.gene033069:CDS
AGCCGGTGTTGCTGTTTCTA+GGG 0.504464 8.2:+16362069 MS.gene033069:CDS
TGAGATTCATCTTGTTCTTG+GGG 0.504648 8.2:+16362519 MS.gene033069:CDS
AGTTGAAGAAGCTGAAGGGA+AGG 0.506548 8.2:+16362480 MS.gene033069:CDS
GAAAAGCTGAGGGGTAAAGA+TGG 0.507731 8.2:+16362106 MS.gene033069:CDS
TGAATTTGAGGAGAGGTTGA+AGG 0.507933 8.2:+16362444 MS.gene033069:CDS
AAAGAAGTTGAAGAAGCTGA+AGG 0.507975 8.2:+16362475 MS.gene033069:CDS
TGGATTAAAAGAGAGATATG+AGG 0.510884 8.2:+16362738 MS.gene033069:CDS
ACAACGGTTTACATTGATGC+TGG 0.514218 8.2:+16364969 MS.gene033069:CDS
CTAATTGTATCCGGAACACT+AGG 0.517001 8.2:-16362706 None:intergenic
CCAAAATGAGAAAGAAAGGT+TGG 0.517592 8.2:+16363847 MS.gene033069:CDS
GAAGTATAGGGGTGAATTTG+AGG 0.518020 8.2:+16362432 MS.gene033069:CDS
AGCTGTCCACGAGCAAGCAT+TGG 0.520046 8.2:-16362586 None:intergenic
TACATTGATGCTGGTCCTAA+GGG 0.520358 8.2:+16364978 MS.gene033069:CDS
TAGCCGATGGACTGGTATAC+CGG 0.521383 8.2:+16363811 MS.gene033069:CDS
AGACTGCACACTAGAGTTGT+GGG 0.523577 8.2:+16363884 MS.gene033069:CDS
AAAAGCTGAGGGGTAAAGAT+GGG 0.524371 8.2:+16362107 MS.gene033069:CDS
GGCGATTGATTTAGTTGATG+AGG 0.524818 8.2:+16362972 MS.gene033069:CDS
GCTTGGAAGAAAATACCGTT+AGG 0.527952 8.2:-16361806 None:intergenic
GCATACCGTGTAGAAAAGAA+TGG 0.529493 8.2:+16365011 MS.gene033069:CDS
ACAACCTCGGCTATTTGGTC+CGG 0.530467 8.2:-16363788 None:intergenic
TATGCTAAATCACTTCCCTT+AGG 0.532281 8.2:-16364993 None:intergenic
TTACTATACTTCATCTTCAG+AGG 0.533219 8.2:-16363551 None:intergenic
ATAGGGGTGAATTTGAGGAG+AGG 0.533373 8.2:+16362437 MS.gene033069:CDS
TTGGCCAAAATGAGAAAGAA+AGG 0.533425 8.2:+16363843 MS.gene033069:CDS
GGTTGCAAGGTTGCAAATGA+AGG 0.533779 8.2:+16364649 MS.gene033069:CDS
AAGGAACCAGTTGGTTGTTG+AGG 0.534620 8.2:-16363971 None:intergenic
GAGGTTGTTAGCCGATGGAC+TGG 0.535262 8.2:+16363803 MS.gene033069:CDS
ATCATCATGACCTCTAACCT+TGG 0.536499 8.2:+16364467 MS.gene033069:CDS
AGGAAATTGACAATCTTGAA+AGG 0.539527 8.2:+16363034 MS.gene033069:CDS
AACCGCGGTTGTTGAAGGGT+TGG 0.544086 8.2:+16362321 MS.gene033069:CDS
CTTATTGGTGAACCTGGTGT+TGG 0.545077 8.2:+16362295 MS.gene033069:CDS
CTAACATCAGCAAGATTGCT+AGG 0.545324 8.2:-16362370 None:intergenic
AGAATTGATGCAAGGTGTAA+GGG 0.547836 8.2:-16361773 None:intergenic
GCCAGAATTGTTGAACCGAC+TGG 0.548964 8.2:+16364580 MS.gene033069:CDS
CAGCAACAGCATTAACCGCC+TGG 0.549246 8.2:-16363913 None:intergenic
ATCACATGAGCAATTGAGAA+AGG 0.549247 8.2:+16364628 MS.gene033069:CDS
AACCCTAGAAACAGCAACAC+CGG 0.556906 8.2:-16362071 None:intergenic
AACTGAGGTTGAAAAGCTGA+GGG 0.557131 8.2:+16362096 MS.gene033069:CDS
GGTATGTTGGACATGAAGAA+GGG 0.557789 8.2:+16364282 MS.gene033069:intron
CTTGGGGCTGGTAGAACAGA+AGG 0.557876 8.2:+16362535 MS.gene033069:CDS
GAGAGATATGAGGGTCATCA+TGG 0.559334 8.2:+16362748 MS.gene033069:CDS
GGATTGTTAATGCTGAAACT+GGG 0.559679 8.2:+16365036 MS.gene033069:CDS
TAGAGTTGTGGGACAAGACC+AGG 0.560454 8.2:+16363895 MS.gene033069:CDS
GAAGACTTATGGAAGAGACT+TGG 0.562344 8.2:+16362174 MS.gene033069:CDS
TGTCAAGAAAATGAGAATTG+AGG 0.563545 8.2:+16365118 MS.gene033069:CDS
CTTCAATTGCACCATATCGC+AGG 0.563940 8.2:-16363682 None:intergenic
AAGAAGTTGAAGAAGCTGAA+GGG 0.564464 8.2:+16362476 MS.gene033069:CDS
TGCTGAGAGAGGAATTGCAT+TGG 0.564643 8.2:+16364688 MS.gene033069:CDS
CATACTTACATCTACAAGAC+GGG 0.566138 8.2:-16363121 None:intergenic
GTTGACTGAAACAGTTGGAC+CGG 0.571247 8.2:+16363769 MS.gene033069:CDS
GGACTGGTATACCGGTGACT+AGG 0.572247 8.2:+16363819 MS.gene033069:CDS
ATCTTGAAGGTAGCACTGAT+GGG 0.576475 8.2:+16363726 MS.gene033069:CDS
GTTAATCTTGGGAATTCTCG+AGG 0.577968 8.2:+16362018 MS.gene033069:CDS
GGTGCTGAGCATCTCTTGAG+TGG 0.579664 8.2:+16364488 MS.gene033069:CDS
CCAGGCACTTCATCTGGTGG+AGG 0.580312 8.2:-16361914 None:intergenic
CAGACTGCACACTAGAGTTG+TGG 0.580593 8.2:+16363883 MS.gene033069:CDS
TAGCCGAGGTTGTTAGCCGA+TGG 0.581783 8.2:+16363798 MS.gene033069:CDS
GGTATACCGGTGACTAGGCT+TGG 0.582136 8.2:+16363824 MS.gene033069:CDS
CCTCCACCAGATGAAGTGCC+TGG 0.583174 8.2:+16361914 MS.gene033069:CDS
TTGCAGCTCAATTGTCTAGT+CGG 0.583231 8.2:+16362800 MS.gene033069:CDS
CTACCAGGCACTTCATCTGG+TGG 0.583721 8.2:-16361917 None:intergenic
TTTAAACCAATGCTTGCTCG+TGG 0.585207 8.2:+16362580 MS.gene033069:CDS
GAGTGATGCAGGAAGTAAGA+AGG 0.587622 8.2:+16364549 MS.gene033069:CDS
TAACAGAAGCTGTAAGGAGA+AGG 0.589440 8.2:+16364312 MS.gene033069:CDS
AGCAATTGAGAAAGGTTGCA+AGG 0.593285 8.2:+16364636 MS.gene033069:CDS
AGAGGATTGATGAGATTCGA+AGG 0.595177 8.2:+16363582 MS.gene033069:CDS
TTGTTGAAGGGTTGGCTCAG+AGG 0.595730 8.2:+16362329 MS.gene033069:CDS
TCGATCGCGAGCAGCTTGCA+TGG 0.599673 8.2:-16364528 None:intergenic
TGGTAAAACTGAGCTTGCAA+AGG 0.600639 8.2:+16364018 MS.gene033069:CDS
ATTCTAGTTTCTGATCCTAA+CGG 0.601379 8.2:+16361791 MS.gene033069:CDS
AAACTGAGGTTGAAAAGCTG+AGG 0.602048 8.2:+16362095 MS.gene033069:CDS
CTGCCTCCTGTAGTGCAAAG+AGG 0.602843 8.2:-16363628 None:intergenic
TATTGGTGCTACAACGCTTG+AGG 0.605062 8.2:+16362615 MS.gene033069:CDS
AGTTGGACCAAGGAATAAGA+AGG 0.606835 8.2:-16363990 None:intergenic
TTAATGTTGACTGAAACAGT+TGG 0.611185 8.2:+16363764 MS.gene033069:CDS
GGCGGTTAATGCTGTTGCTG+AGG 0.611492 8.2:+16363916 MS.gene033069:CDS
TGTTTCTAGGGTTAAAACTG+AGG 0.616506 8.2:+16362081 MS.gene033069:CDS
TACAAGTCTTGGATGATGGG+AGG 0.616755 8.2:+16364399 MS.gene033069:CDS
TACCGTGTAGAAAAGAATGG+AGG 0.617392 8.2:+16365014 MS.gene033069:CDS
GGATTAAAAGAGAGATATGA+GGG 0.617828 8.2:+16362739 MS.gene033069:CDS
CAATTCCTCTCTCAGCAAGA+CGG 0.617980 8.2:-16364682 None:intergenic
GCTAGCCGTCTTGCTGAGAG+AGG 0.619045 8.2:+16364677 MS.gene033069:CDS
CCATACTTACATCTACAAGA+CGG 0.619404 8.2:-16363122 None:intergenic
TACCAACACCAGTTGGACCA+AGG 0.623035 8.2:-16364000 None:intergenic
TCATGCTCTAGAGAAAGAGA+AGG 0.626681 8.2:+16363083 MS.gene033069:CDS
GAGACTTGGTTGAACAAGCA+GGG 0.628162 8.2:+16362188 MS.gene033069:CDS
ATTTGATGATGAAAATCAGT+TGG 0.628949 8.2:+16364057 MS.gene033069:CDS
ACCGTGTAGAAAAGAATGGA+GGG 0.629952 8.2:+16365015 MS.gene033069:CDS
AGGAATAAGAAGGAACCAGT+TGG 0.632548 8.2:-16363980 None:intergenic
GGGAGGTTGACTGACGGACA+AGG 0.633232 8.2:+16364416 MS.gene033069:CDS
GCTGTATTGAGATCAAGAGC+TGG 0.635226 8.2:+16363938 MS.gene033069:CDS
TCTGGTGGAGGTGATTGAGA+TGG 0.640106 8.2:-16361902 None:intergenic
AAGAGATTCGAAGAGTTGTG+AGG 0.646944 8.2:+16362239 MS.gene033069:CDS
GTTGGTGAGAATTGACATGT+CGG 0.648441 8.2:+16364075 MS.gene033069:CDS
ATGGTGCTAGACCAATTAGA+AGG 0.648738 8.2:+16364880 MS.gene033069:CDS
AGGGCGTCATCTACCTGACA+AGG 0.649063 8.2:+16362951 MS.gene033069:intron
AGCCAACCCTTCAACAACCG+CGG 0.649201 8.2:-16362323 None:intergenic
CAGAAACTAGAATTGATGCA+AGG 0.649347 8.2:-16361781 None:intergenic
TGGTAGAACAGAAGGATCGA+TGG 0.650995 8.2:+16362543 MS.gene033069:CDS
GTATGTTGGACATGAAGAAG+GGG 0.651475 8.2:+16364283 MS.gene033069:CDS
TGCATTGGCAGTAACAGATG+CGG 0.653933 8.2:+16364703 MS.gene033069:CDS
ATATGGTGCAATTGAAGAGG+TGG 0.654225 8.2:+16363688 MS.gene033069:CDS
TCACCGGTATACCAGTCCAT+CGG 0.655399 8.2:-16363814 None:intergenic
AGAAATTGATGAAAATACAA+CGG 0.657908 8.2:+16364953 MS.gene033069:CDS
AAGGTTGCAAATGAAGGATG+TGG 0.660475 8.2:+16364655 MS.gene033069:CDS
ACGAATCGCTTTGATAAGCG+CGG 0.664960 8.2:-16361942 None:intergenic
ATACAACAACTTCGTCCAGT+CGG 0.665392 8.2:-16364595 None:intergenic
GTTGCTGGTGCGAAGTATAG+GGG 0.666198 8.2:+16362421 MS.gene033069:CDS
TCAAGAGATGCTCAGCACCA+AGG 0.666994 8.2:-16364484 None:intergenic
AGTCCATCGGCTAACAACCT+CGG 0.668166 8.2:-16363801 None:intergenic
CACTACAGGAGGCAGAGAGG+CGG 0.668262 8.2:+16363636 MS.gene033069:CDS
GTGCTAGACCAATTAGAAGG+TGG 0.668833 8.2:+16364883 MS.gene033069:CDS
GCTCCTCTTTGCACTACAGG+AGG 0.670257 8.2:+16363625 MS.gene033069:CDS
GATTGTTAATGCTGAAACTG+GGG 0.676690 8.2:+16365037 MS.gene033069:CDS
TGACGGACAAGGCAGAACTG+TGG 0.678310 8.2:+16364427 MS.gene033069:CDS
GTATGATCTAGCCAGAGCTG+CGG 0.683383 8.2:+16363658 MS.gene033069:CDS
TCAACTTGATAGTCAGCCTG+AGG 0.685328 8.2:+16363014 MS.gene033069:CDS
GATTGATTGGTGCACCACCA+GGG 0.685797 8.2:+16364125 MS.gene033069:CDS
GCGATATGGTGCAATTGAAG+AGG 0.687694 8.2:+16363685 MS.gene033069:CDS
CAAGCTGCACAGAAATCACG+TGG 0.691575 8.2:+16361971 MS.gene033069:CDS
AATCTAATAACTTACCCTGG+TGG 0.704597 8.2:-16364139 None:intergenic
TGGACCGGACCAAATAGCCG+AGG 0.708477 8.2:+16363784 MS.gene033069:CDS
TATGTTGGACATGAAGAAGG+GGG 0.714139 8.2:+16364284 MS.gene033069:CDS
TTGCACTACAGGAGGCAGAG+AGG 0.720314 8.2:+16363633 MS.gene033069:CDS
GATGATGGGAGGTTGACTGA+CGG 0.725487 8.2:+16364410 MS.gene033069:CDS
ACTGAGGTTGAAAAGCTGAG+GGG 0.737026 8.2:+16362097 MS.gene033069:CDS
AGTTGTGGGACAAGACCAGG+CGG 0.784379 8.2:+16363898 MS.gene033069:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATATCATCTTAATCTTT+AGG + chr8.2:16364778-16364797 MS.gene033069:intron 15.0%
!! GAATAAAATTTGACTAAATA+AGG - chr8.2:16363261-16363280 None:intergenic 15.0%
!! TATAATATAATAACACTTAC+TGG - chr8.2:16364761-16364780 None:intergenic 15.0%
!!! TATTAGTCTAAATAATATTC+AGG - chr8.2:16364169-16364188 None:intergenic 15.0%
!! AGAAATTGATGAAAATACAA+CGG + chr8.2:16364953-16364972 MS.gene033069:CDS 20.0%
!! ATAGAATATTTCAGACAAAA+TGG + chr8.2:16364803-16364822 MS.gene033069:intron 20.0%
!! TTAATATACATTCTAGTATG+TGG + chr8.2:16364220-16364239 MS.gene033069:intron 20.0%
! AATGGTTTAAATTGTGTATG+TGG + chr8.2:16363401-16363420 MS.gene033069:intron 25.0%
! ACTGCAATTAAAAATCTTGA+AGG + chr8.2:16363713-16363732 MS.gene033069:CDS 25.0%
! AGAGAATATAAAGAACATAC+CGG - chr8.2:16362833-16362852 None:intergenic 25.0%
! ATGGTTTAAATTGTGTATGT+GGG + chr8.2:16363402-16363421 MS.gene033069:intron 25.0%
! CATCAAAATTAATGCTATCA+TGG - chr8.2:16363193-16363212 None:intergenic 25.0%
! CTAAGTACATAACTATATTG+TGG + chr8.2:16363358-16363377 MS.gene033069:intron 25.0%
!! ATGTGTTTTGTATGTGTTAA+GGG + chr8.2:16362932-16362951 MS.gene033069:intron 25.0%
!! TATGTGTTTTGTATGTGTTA+AGG + chr8.2:16362931-16362950 MS.gene033069:intron 25.0%
!! TCATTTTGCATTGAATTTTC+AGG + chr8.2:16364261-16364280 MS.gene033069:intron 25.0%
!!! ATTTGATGATGAAAATCAGT+TGG + chr8.2:16364057-16364076 MS.gene033069:CDS 25.0%
AAATTGTGTATGTGGGATAA+TGG + chr8.2:16363409-16363428 MS.gene033069:intron 30.0%
AAGAACAATCCTGTTCTTAT+TGG + chr8.2:16362280-16362299 MS.gene033069:CDS 30.0%
AGGAAATTGACAATCTTGAA+AGG + chr8.2:16363034-16363053 MS.gene033069:CDS 30.0%
AGTACATAACTATATTGTGG+TGG + chr8.2:16363361-16363380 MS.gene033069:intron 30.0%
AGTATAGTAAAGAGAAAGAG+AGG + chr8.2:16363564-16363583 MS.gene033069:CDS 30.0%
ATGAGATTCATCTTGTTCTT+GGG + chr8.2:16362518-16362537 MS.gene033069:CDS 30.0%
ATTCTGATTCAGATTCCAAA+TGG + chr8.2:16365071-16365090 MS.gene033069:CDS 30.0%
ATTTGAAAGAAGGTTTCAAC+AGG + chr8.2:16362669-16362688 MS.gene033069:CDS 30.0%
GGATTAAAAGAGAGATATGA+GGG + chr8.2:16362739-16362758 MS.gene033069:CDS 30.0%
TAGTGGCTAATAATACTAAC+TGG + chr8.2:16364826-16364845 MS.gene033069:intron 30.0%
TATTTCAGACAAAATGGTAG+TGG + chr8.2:16364809-16364828 MS.gene033069:intron 30.0%
TGATGTTAGGTTAATTGCTT+TGG + chr8.2:16362384-16362403 MS.gene033069:CDS 30.0%
TGGATTAAAAGAGAGATATG+AGG + chr8.2:16362738-16362757 MS.gene033069:CDS 30.0%
TGTCAAGAAAATGAGAATTG+AGG + chr8.2:16365118-16365137 MS.gene033069:CDS 30.0%
TTACTATACTTCATCTTCAG+AGG - chr8.2:16363554-16363573 None:intergenic 30.0%
! AGGTTAATTGCTTTGGATAT+GGG + chr8.2:16362391-16362410 MS.gene033069:CDS 30.0%
! GTTTTGTGAGTAAATTTCTC+TGG - chr8.2:16361692-16361711 None:intergenic 30.0%
! TAGGTTAATTGCTTTGGATA+TGG + chr8.2:16362390-16362409 MS.gene033069:CDS 30.0%
! TCAAAAAGTACAACACTGTA+TGG - chr8.2:16364338-16364357 None:intergenic 30.0%
! TTAACTCTAGCTACTAGTTT+AGG - chr8.2:16364193-16364212 None:intergenic 30.0%
! TTAATGTTGACTGAAACAGT+TGG + chr8.2:16363764-16363783 MS.gene033069:CDS 30.0%
!! ATTCTAGTTTCTGATCCTAA+CGG + chr8.2:16361791-16361810 MS.gene033069:CDS 30.0%
!! GTTATTGTTGTTTCTTGTGT+AGG + chr8.2:16364854-16364873 MS.gene033069:intron 30.0%
!! TTTCAAGCATTGAAGACTTA+TGG + chr8.2:16362163-16362182 MS.gene033069:CDS 30.0%
!!! CATTGAATTTTCAGGTATGT+TGG + chr8.2:16364269-16364288 MS.gene033069:intron 30.0%
AAAGAAGTTGAAGAAGCTGA+AGG + chr8.2:16362475-16362494 MS.gene033069:CDS 35.0%
AAGAAGTTGAAGAAGCTGAA+GGG + chr8.2:16362476-16362495 MS.gene033069:CDS 35.0%
AAGATGCAGCATTTGAAAGA+AGG + chr8.2:16362659-16362678 MS.gene033069:CDS 35.0%
AAGGTTTCAACAGGTTTATG+TGG + chr8.2:16362678-16362697 MS.gene033069:CDS 35.0%
AATCTAATAACTTACCCTGG+TGG - chr8.2:16364142-16364161 None:intergenic 35.0%
ACGAATCTAATAACTTACCC+TGG - chr8.2:16364145-16364164 None:intergenic 35.0%
AGAATTGATGCAAGGTGTAA+GGG - chr8.2:16361776-16361795 None:intergenic 35.0%
AGCTGTTGATCAGTTAATCT+TGG + chr8.2:16362006-16362025 MS.gene033069:CDS 35.0%
ATCACATGAGCAATTGAGAA+AGG + chr8.2:16364628-16364647 MS.gene033069:CDS 35.0%
CACTCTGTATCAAGATTGAT+TGG + chr8.2:16364112-16364131 MS.gene033069:CDS 35.0%
CACTTGTGTTTATGCTAACA+TGG + chr8.2:16362908-16362927 MS.gene033069:intron 35.0%
CAGAAACTAGAATTGATGCA+AGG - chr8.2:16361784-16361803 None:intergenic 35.0%
CATACTTACATCTACAAGAC+GGG - chr8.2:16363124-16363143 None:intergenic 35.0%
CCAAAATGAGAAAGAAAGGT+TGG + chr8.2:16363847-16363866 MS.gene033069:CDS 35.0%
CCATACTTACATCTACAAGA+CGG - chr8.2:16363125-16363144 None:intergenic 35.0%
CGAAGAATGCTAATTGTATC+CGG - chr8.2:16362718-16362737 None:intergenic 35.0%
GATACAATTAGCATTCTTCG+TGG + chr8.2:16362718-16362737 MS.gene033069:CDS 35.0%
GATGAGATTCATCTTGTTCT+TGG + chr8.2:16362517-16362536 MS.gene033069:CDS 35.0%
GATTGTTAATGCTGAAACTG+GGG + chr8.2:16365037-16365056 MS.gene033069:CDS 35.0%
GCTGTTGATCAGTTAATCTT+GGG + chr8.2:16362007-16362026 MS.gene033069:CDS 35.0%
GGATTGTTAATGCTGAAACT+GGG + chr8.2:16365036-16365055 MS.gene033069:CDS 35.0%
GTCAATTAACAGAAGCTGTA+AGG + chr8.2:16364306-16364325 MS.gene033069:CDS 35.0%
TAGAATTGATGCAAGGTGTA+AGG - chr8.2:16361777-16361796 None:intergenic 35.0%
TATGCTAAATCACTTCCCTT+AGG - chr8.2:16364996-16365015 None:intergenic 35.0%
TCAAGATTGTCAATTTCCTC+AGG - chr8.2:16363033-16363052 None:intergenic 35.0%
TGAGATTCATCTTGTTCTTG+GGG + chr8.2:16362519-16362538 MS.gene033069:CDS 35.0%
TTCTACAAGTCTTGGATGAT+GGG + chr8.2:16364396-16364415 MS.gene033069:CDS 35.0%
TTGGCCAAAATGAGAAAGAA+AGG + chr8.2:16363843-16363862 MS.gene033069:CDS 35.0%
! AATGAGAAAGAAAGGTTGGT+TGG + chr8.2:16363851-16363870 MS.gene033069:CDS 35.0%
! CCAACCTTTCTTTCTCATTT+TGG - chr8.2:16363850-16363869 None:intergenic 35.0%
! TGATAGTTGATGTTATTCGC+AGG + chr8.2:16363490-16363509 MS.gene033069:intron 35.0%
! TTGTGAGGATTTTGTCAAGA+AGG + chr8.2:16362254-16362273 MS.gene033069:CDS 35.0%
! TTGTTTCTTGTGTAGGTGTA+TGG + chr8.2:16364861-16364880 MS.gene033069:intron 35.0%
!! AAAAAGGTTGAAAGTGCTTC+TGG + chr8.2:16362130-16362149 MS.gene033069:CDS 35.0%
!! GGAAGTTGATGATGATGAAA+TGG + chr8.2:16365139-16365158 MS.gene033069:CDS 35.0%
!! GGTGATTTGTTTAAAGAAGC+CGG + chr8.2:16362052-16362071 MS.gene033069:CDS 35.0%
!! TGTTTCTAGGGTTAAAACTG+AGG + chr8.2:16362081-16362100 MS.gene033069:CDS 35.0%
AAAAGCTGAGGGGTAAAGAT+GGG + chr8.2:16362107-16362126 MS.gene033069:CDS 40.0%
AAACTGAGGTTGAAAAGCTG+AGG + chr8.2:16362095-16362114 MS.gene033069:CDS 40.0%
AACTGAGGTTGAAAAGCTGA+GGG + chr8.2:16362096-16362115 MS.gene033069:CDS 40.0%
AAGAGATTCGAAGAGTTGTG+AGG + chr8.2:16362239-16362258 MS.gene033069:CDS 40.0%
AAGGTTGCAAATGAAGGATG+TGG + chr8.2:16364655-16364674 MS.gene033069:CDS 40.0%
AATTGACATGTCGGAGTACA+TGG + chr8.2:16364084-16364103 MS.gene033069:CDS 40.0%
ACAACGGTTTACATTGATGC+TGG + chr8.2:16364969-16364988 MS.gene033069:CDS 40.0%
ACCGTGTAGAAAAGAATGGA+GGG + chr8.2:16365015-16365034 MS.gene033069:CDS 40.0%
ACTAAATCAATCGCCTTGTC+AGG - chr8.2:16362967-16362986 None:intergenic 40.0%
AGAGGATTGATGAGATTCGA+AGG + chr8.2:16363582-16363601 MS.gene033069:CDS 40.0%
AGCAATTGAGAAAGGTTGCA+AGG + chr8.2:16364636-16364655 MS.gene033069:CDS 40.0%
AGGAATAAGAAGGAACCAGT+TGG - chr8.2:16363983-16364002 None:intergenic 40.0%
AGGTATGTTGGACATGAAGA+AGG + chr8.2:16364281-16364300 MS.gene033069:intron 40.0%
AGTTGGACCAAGGAATAAGA+AGG - chr8.2:16363993-16364012 None:intergenic 40.0%
ATACAACAACTTCGTCCAGT+CGG - chr8.2:16364598-16364617 None:intergenic 40.0%
ATATGGTGCAATTGAAGAGG+TGG + chr8.2:16363688-16363707 MS.gene033069:CDS 40.0%
ATCATCATGACCTCTAACCT+TGG + chr8.2:16364467-16364486 MS.gene033069:CDS 40.0%
ATTCTCGAGGATTCTCAGAT+TGG + chr8.2:16362031-16362050 MS.gene033069:CDS 40.0%
CAACACTCTTCTACAAGTCT+TGG + chr8.2:16364388-16364407 MS.gene033069:CDS 40.0%
CAAGCAATTTCAAACGTTGC+TGG + chr8.2:16361824-16361843 MS.gene033069:CDS 40.0%
CCGTCTTGTAGATGTAAGTA+TGG + chr8.2:16363122-16363141 MS.gene033069:intron 40.0%
CTAACATCAGCAAGATTGCT+AGG - chr8.2:16362373-16362392 None:intergenic 40.0%
CTAATTGTATCCGGAACACT+AGG - chr8.2:16362709-16362728 None:intergenic 40.0%
CTAGCAATCTTGCTGATGTT+AGG + chr8.2:16362371-16362390 MS.gene033069:CDS 40.0%
CTCAATTGCTCATGTGATAG+AGG - chr8.2:16364626-16364645 None:intergenic 40.0%
CTTCTACAAGTCTTGGATGA+TGG + chr8.2:16364395-16364414 MS.gene033069:CDS 40.0%
GAAGACTTATGGAAGAGACT+TGG + chr8.2:16362174-16362193 MS.gene033069:CDS 40.0%
GAAGTATAGGGGTGAATTTG+AGG + chr8.2:16362432-16362451 MS.gene033069:CDS 40.0%
GCATACCGTGTAGAAAAGAA+TGG + chr8.2:16365011-16365030 MS.gene033069:CDS 40.0%
GCTTGGAAGAAAATACCGTT+AGG - chr8.2:16361809-16361828 None:intergenic 40.0%
GGGATTGTTAATGCTGAAAC+TGG + chr8.2:16365035-16365054 MS.gene033069:CDS 40.0%
GGTATGTTGGACATGAAGAA+GGG + chr8.2:16364282-16364301 MS.gene033069:intron 40.0%
GTATGTTGGACATGAAGAAG+GGG + chr8.2:16364283-16364302 MS.gene033069:CDS 40.0%
GTTAATCTTGGGAATTCTCG+AGG + chr8.2:16362018-16362037 MS.gene033069:CDS 40.0%
GTTGATGTTATTCGCAGGTA+CGG + chr8.2:16363495-16363514 MS.gene033069:intron 40.0%
TAACAGAAGCTGTAAGGAGA+AGG + chr8.2:16364312-16364331 MS.gene033069:CDS 40.0%
TACATTGATGCTGGTCCTAA+GGG + chr8.2:16364978-16364997 MS.gene033069:CDS 40.0%
TACCGTGTAGAAAAGAATGG+AGG + chr8.2:16365014-16365033 MS.gene033069:CDS 40.0%
TATGTTGGACATGAAGAAGG+GGG + chr8.2:16364284-16364303 MS.gene033069:CDS 40.0%
TGAATTTGAGGAGAGGTTGA+AGG + chr8.2:16362444-16362463 MS.gene033069:CDS 40.0%
TGGTAAAACTGAGCTTGCAA+AGG + chr8.2:16364018-16364037 MS.gene033069:CDS 40.0%
TTACATTGATGCTGGTCCTA+AGG + chr8.2:16364977-16364996 MS.gene033069:CDS 40.0%
TTCTTATTCCTTGGTCCAAC+TGG + chr8.2:16363992-16364011 MS.gene033069:CDS 40.0%
TTGCAGCTCAATTGTCTAGT+CGG + chr8.2:16362800-16362819 MS.gene033069:CDS 40.0%
TTGTCTAGTCGGTACATAAC+CGG + chr8.2:16362811-16362830 MS.gene033069:CDS 40.0%
TTTAAACCAATGCTTGCTCG+TGG + chr8.2:16362580-16362599 MS.gene033069:CDS 40.0%
! AAAACAAACGAAGCACTTGC+TGG + chr8.2:16361707-16361726 MS.gene033069:CDS 40.0%
! AAAGAAAGGTTGGTTGGACT+TGG + chr8.2:16363857-16363876 MS.gene033069:CDS 40.0%
! AATCTTGAAGGTAGCACTGA+TGG + chr8.2:16363725-16363744 MS.gene033069:CDS 40.0%
! ACTGGTTCCTTCTTATTCCT+TGG + chr8.2:16363983-16364002 MS.gene033069:CDS 40.0%
! ATGGTGCTAGACCAATTAGA+AGG + chr8.2:16364880-16364899 MS.gene033069:CDS 40.0%
! CAGCAACGTTTGAAATTGCT+TGG - chr8.2:16361826-16361845 None:intergenic 40.0%
! GGCGATTGATTTAGTTGATG+AGG + chr8.2:16362972-16362991 MS.gene033069:CDS 40.0%
! GTTGGTGAGAATTGACATGT+CGG + chr8.2:16364075-16364094 MS.gene033069:CDS 40.0%
! TCAGTTTTACCAACACCAGT+TGG - chr8.2:16364010-16364029 None:intergenic 40.0%
! TCCCTCCATTCTTTTCTACA+CGG - chr8.2:16365019-16365038 None:intergenic 40.0%
! TTGAGATCAAGAGCTGGTTT+AGG + chr8.2:16363944-16363963 MS.gene033069:CDS 40.0%
!! AGGAAGTAAGAAGGCATTTC+AGG + chr8.2:16364558-16364577 MS.gene033069:CDS 40.0%
!! ATCTTGAAGGTAGCACTGAT+GGG + chr8.2:16363726-16363745 MS.gene033069:CDS 40.0%
!! TCATGCTCTAGAGAAAGAGA+AGG + chr8.2:16363083-16363102 MS.gene033069:CDS 40.0%
AACCCTAGAAACAGCAACAC+CGG - chr8.2:16362074-16362093 None:intergenic 45.0%
AAGGAACCAGTTGGTTGTTG+AGG - chr8.2:16363974-16363993 None:intergenic 45.0%
AAGGCAGAACTGTGGACTTT+AGG + chr8.2:16364435-16364454 MS.gene033069:CDS 45.0%
ACCGAAAAGTGATGCTGTTC+AGG + chr8.2:16365094-16365113 MS.gene033069:CDS 45.0%
ACGAATCGCTTTGATAAGCG+CGG - chr8.2:16361945-16361964 None:intergenic 45.0%
ACTGAGGTTGAAAAGCTGAG+GGG + chr8.2:16362097-16362116 MS.gene033069:CDS 45.0%
AGACTGCACACTAGAGTTGT+GGG + chr8.2:16363884-16363903 MS.gene033069:CDS 45.0%
AGTTGAAGAAGCTGAAGGGA+AGG + chr8.2:16362480-16362499 MS.gene033069:CDS 45.0%
ATAGGGGTGAATTTGAGGAG+AGG + chr8.2:16362437-16362456 MS.gene033069:CDS 45.0%
ATTCATCTTGTTCTTGGGGC+TGG + chr8.2:16362523-16362542 MS.gene033069:CDS 45.0%
CAATTAGAAGGTGGCTAGAG+AGG + chr8.2:16364892-16364911 MS.gene033069:CDS 45.0%
CAATTCCTCTCTCAGCAAGA+CGG - chr8.2:16364685-16364704 None:intergenic 45.0%
CCAGGTTCACCAATAAGAAC+AGG - chr8.2:16362292-16362311 None:intergenic 45.0%
CCTGTTCTTATTGGTGAACC+TGG + chr8.2:16362289-16362308 MS.gene033069:CDS 45.0%
CTCTCTAGCCACCTTCTAAT+TGG - chr8.2:16364894-16364913 None:intergenic 45.0%
CTTATTGGTGAACCTGGTGT+TGG + chr8.2:16362295-16362314 MS.gene033069:CDS 45.0%
CTTCAATTGCACCATATCGC+AGG - chr8.2:16363685-16363704 None:intergenic 45.0%
GAAAAACCGCGGTTGTTGAA+GGG + chr8.2:16362317-16362336 MS.gene033069:CDS 45.0%
GAAAAGCTGAGGGGTAAAGA+TGG + chr8.2:16362106-16362125 MS.gene033069:CDS 45.0%
GAGAGATATGAGGGTCATCA+TGG + chr8.2:16362748-16362767 MS.gene033069:CDS 45.0%
GAGGGGTAAAGATGGGAAAA+AGG + chr8.2:16362114-16362133 MS.gene033069:CDS 45.0%
GAGTGATGCAGGAAGTAAGA+AGG + chr8.2:16364549-16364568 MS.gene033069:CDS 45.0%
GCGATATGGTGCAATTGAAG+AGG + chr8.2:16363685-16363704 MS.gene033069:CDS 45.0%
GCTGTATTGAGATCAAGAGC+TGG + chr8.2:16363938-16363957 MS.gene033069:CDS 45.0%
GGGAAGCTTGATCCTGTTAT+TGG + chr8.2:16362208-16362227 MS.gene033069:CDS 45.0%
GGTTGCAAGGTTGCAAATGA+AGG + chr8.2:16364649-16364668 MS.gene033069:CDS 45.0%
GTGCTAGACCAATTAGAAGG+TGG + chr8.2:16364883-16364902 MS.gene033069:CDS 45.0%
GTGCTGCAAATGTGGGATAA+TGG + chr8.2:16363383-16363402 MS.gene033069:intron 45.0%
GTTGACTGAAACAGTTGGAC+CGG + chr8.2:16363769-16363788 MS.gene033069:CDS 45.0%
TGCTGAGAGAGGAATTGCAT+TGG + chr8.2:16364688-16364707 MS.gene033069:CDS 45.0%
TGGTAGAACAGAAGGATCGA+TGG + chr8.2:16362543-16362562 MS.gene033069:CDS 45.0%
TTGGCTCAGAGGATTGTTAG+AGG + chr8.2:16362340-16362359 MS.gene033069:CDS 45.0%
! AGAGACTTGGTTGAACAAGC+AGG + chr8.2:16362187-16362206 MS.gene033069:CDS 45.0%
! CATCTCTTGAGTGGACTTTC+AGG + chr8.2:16364497-16364516 MS.gene033069:CDS 45.0%
! CTACAACGCTTGAGGAGTAT+AGG + chr8.2:16362623-16362642 MS.gene033069:CDS 45.0%
! GAGACTTGGTTGAACAAGCA+GGG + chr8.2:16362188-16362207 MS.gene033069:CDS 45.0%
! TCAACTTGATAGTCAGCCTG+AGG + chr8.2:16363014-16363033 MS.gene033069:CDS 45.0%
! TCCAGTCGGTTCAACAATTC+TGG - chr8.2:16364584-16364603 None:intergenic 45.0%
! TGCATTGGCAGTAACAGATG+CGG + chr8.2:16364703-16364722 MS.gene033069:CDS 45.0%
!! GCATCACTTTTCGGTCCATT+TGG - chr8.2:16365089-16365108 None:intergenic 45.0%
!! GCCTGAACAGCATCACTTTT+CGG - chr8.2:16365098-16365117 None:intergenic 45.0%
!! TACAAGTCTTGGATGATGGG+AGG + chr8.2:16364399-16364418 MS.gene033069:CDS 45.0%
!! TATTGGTGCTACAACGCTTG+AGG + chr8.2:16362615-16362634 MS.gene033069:CDS 45.0%
ACAACCTCGGCTATTTGGTC+CGG - chr8.2:16363791-16363810 None:intergenic 50.0%
AGAGCTCCTCTTTGCACTAC+AGG + chr8.2:16363622-16363641 MS.gene033069:CDS 50.0%
AGATTGATTGGTGCACCACC+AGG + chr8.2:16364124-16364143 MS.gene033069:CDS 50.0%
AGGTTGTGACAGAGCTGTCT+AGG + chr8.2:16364916-16364935 MS.gene033069:CDS 50.0%
ATGCTCAGCACCAAGGTTAG+AGG - chr8.2:16364480-16364499 None:intergenic 50.0%
CAAGCTGCACAGAAATCACG+TGG + chr8.2:16361971-16361990 MS.gene033069:CDS 50.0%
CACGAGCTAGCAATGACTTC+AGG + chr8.2:16361734-16361753 MS.gene033069:CDS 50.0%
CAGACTGCACACTAGAGTTG+TGG + chr8.2:16363883-16363902 MS.gene033069:CDS 50.0%
CGTGGACAGCTTCGATGTAT+TGG + chr8.2:16362598-16362617 MS.gene033069:CDS 50.0%
GATTGATTGGTGCACCACCA+GGG + chr8.2:16364125-16364144 MS.gene033069:CDS 50.0%
GCCAGAATTGTTGAACCGAC+TGG + chr8.2:16364580-16364599 MS.gene033069:CDS 50.0%
GGAAAAACCGCGGTTGTTGA+AGG + chr8.2:16362316-16362335 MS.gene033069:CDS 50.0%
GGAAGACCTCAACAACCAAC+TGG + chr8.2:16363965-16363984 MS.gene033069:CDS 50.0%
GGCTAACAACCTCGGCTATT+TGG - chr8.2:16363796-16363815 None:intergenic 50.0%
GGTTGCTGGTGCGAAGTATA+GGG + chr8.2:16362420-16362439 MS.gene033069:CDS 50.0%
GTATGATCTAGCCAGAGCTG+CGG + chr8.2:16363658-16363677 MS.gene033069:CDS 50.0%
GTTGCTGGTGCGAAGTATAG+GGG + chr8.2:16362421-16362440 MS.gene033069:CDS 50.0%
TACCAACACCAGTTGGACCA+AGG - chr8.2:16364003-16364022 None:intergenic 50.0%
TAGAAGGTGGCTAGAGAGGA+AGG + chr8.2:16364896-16364915 MS.gene033069:CDS 50.0%
TAGAGTTGTGGGACAAGACC+AGG + chr8.2:16363895-16363914 MS.gene033069:CDS 50.0%
TCAAGAGATGCTCAGCACCA+AGG - chr8.2:16364487-16364506 None:intergenic 50.0%
TCACCGGTATACCAGTCCAT+CGG - chr8.2:16363817-16363836 None:intergenic 50.0%
TCTGGTGGAGGTGATTGAGA+TGG - chr8.2:16361905-16361924 None:intergenic 50.0%
TCTTCGTCGCGTCCAATAAC+AGG - chr8.2:16362223-16362242 None:intergenic 50.0%
TGGTTGCTGGTGCGAAGTAT+AGG + chr8.2:16362419-16362438 MS.gene033069:CDS 50.0%
TGTGGCTGAACCTAGTGTTC+CGG + chr8.2:16362696-16362715 MS.gene033069:CDS 50.0%
TTCCTTGGTCCAACTGGTGT+TGG + chr8.2:16363998-16364017 MS.gene033069:CDS 50.0%
! ACCTGGTGTTGGAAAAACCG+CGG + chr8.2:16362306-16362325 MS.gene033069:CDS 50.0%
! AGTCCATCGGCTAACAACCT+CGG - chr8.2:16363804-16363823 None:intergenic 50.0%
! TGCTTTGGATATGGGAGCAC+TGG + chr8.2:16362399-16362418 MS.gene033069:CDS 50.0%
! TTTTGGCCAAGCCTAGTCAC+CGG - chr8.2:16363833-16363852 None:intergenic 50.0%
!! AAGCCGGTGTTGCTGTTTCT+AGG + chr8.2:16362068-16362087 MS.gene033069:CDS 50.0%
!! AGCCGGTGTTGCTGTTTCTA+GGG + chr8.2:16362069-16362088 MS.gene033069:CDS 50.0%
!! GATGATGGGAGGTTGACTGA+CGG + chr8.2:16364410-16364429 MS.gene033069:CDS 50.0%
!! TAGCCGATGGACTGGTATAC+CGG + chr8.2:16363811-16363830 MS.gene033069:CDS 50.0%
!! TGTGGTGGTGCTGCAAATGT+GGG + chr8.2:16363376-16363395 MS.gene033069:intron 50.0%
!! TTGTGGTGGTGCTGCAAATG+TGG + chr8.2:16363375-16363394 MS.gene033069:intron 50.0%
!! TTGTTGAAGGGTTGGCTCAG+AGG + chr8.2:16362329-16362348 MS.gene033069:CDS 50.0%
AACCGCGGTTGTTGAAGGGT+TGG + chr8.2:16362321-16362340 MS.gene033069:CDS 55.0%
AGCCAACCCTTCAACAACCG+CGG - chr8.2:16362326-16362345 None:intergenic 55.0%
AGCTGTCCACGAGCAAGCAT+TGG - chr8.2:16362589-16362608 None:intergenic 55.0%
AGTTGTGGGACAAGACCAGG+CGG + chr8.2:16363898-16363917 MS.gene033069:CDS 55.0%
CAGCAACAGCATTAACCGCC+TGG - chr8.2:16363916-16363935 None:intergenic 55.0%
CTACCAGGCACTTCATCTGG+TGG - chr8.2:16361920-16361939 None:intergenic 55.0%
CTGCCTCCTGTAGTGCAAAG+AGG - chr8.2:16363631-16363650 None:intergenic 55.0%
GAGGTTGTTAGCCGATGGAC+TGG + chr8.2:16363803-16363822 MS.gene033069:CDS 55.0%
GCTCCTCTTTGCACTACAGG+AGG + chr8.2:16363625-16363644 MS.gene033069:CDS 55.0%
GGCGGTTAATGCTGTTGCTG+AGG + chr8.2:16363916-16363935 MS.gene033069:CDS 55.0%
GGTATACCGGTGACTAGGCT+TGG + chr8.2:16363824-16363843 MS.gene033069:CDS 55.0%
GTGCTACCAGGCACTTCATC+TGG - chr8.2:16361923-16361942 None:intergenic 55.0%
TAGCCGAGGTTGTTAGCCGA+TGG + chr8.2:16363798-16363817 MS.gene033069:CDS 55.0%
TTGCACTACAGGAGGCAGAG+AGG + chr8.2:16363633-16363652 MS.gene033069:CDS 55.0%
! ACCGCGGTTTTTCCAACACC+AGG - chr8.2:16362310-16362329 None:intergenic 55.0%
! AGGGCGTCATCTACCTGACA+AGG + chr8.2:16362951-16362970 MS.gene033069:intron 55.0%
! CTTGGGGCTGGTAGAACAGA+AGG + chr8.2:16362535-16362554 MS.gene033069:CDS 55.0%
! GGACTGGTATACCGGTGACT+AGG + chr8.2:16363819-16363838 MS.gene033069:CDS 55.0%
! GGTGCTGAGCATCTCTTGAG+TGG + chr8.2:16364488-16364507 MS.gene033069:CDS 55.0%
! TGACGGACAAGGCAGAACTG+TGG + chr8.2:16364427-16364446 MS.gene033069:CDS 55.0%
!! GATATGGGAGCACTGGTTGC+TGG + chr8.2:16362406-16362425 MS.gene033069:CDS 55.0%
!! TTGATAAGCGCGGTGCTACC+AGG - chr8.2:16361935-16361954 None:intergenic 55.0%
AGAGCTGCGGACCTGCGATA+TGG + chr8.2:16363671-16363690 MS.gene033069:CDS 60.0%
ATATCGCAGGTCCGCAGCTC+TGG - chr8.2:16363672-16363691 None:intergenic 60.0%
CACTACAGGAGGCAGAGAGG+CGG + chr8.2:16363636-16363655 MS.gene033069:CDS 60.0%
CCAGGCACTTCATCTGGTGG+AGG - chr8.2:16361917-16361936 None:intergenic 60.0%
GCTAGCCGTCTTGCTGAGAG+AGG + chr8.2:16364677-16364696 MS.gene033069:CDS 60.0%
TCGATCGCGAGCAGCTTGCA+TGG - chr8.2:16364531-16364550 None:intergenic 60.0%
TGCTCGCGATCGAGTGATGC+AGG + chr8.2:16364538-16364557 MS.gene033069:CDS 60.0%
TGGACCGGACCAAATAGCCG+AGG + chr8.2:16363784-16363803 MS.gene033069:CDS 60.0%
! CCTCCACCAGATGAAGTGCC+TGG + chr8.2:16361914-16361933 MS.gene033069:CDS 60.0%
! GGGAGGTTGACTGACGGACA+AGG + chr8.2:16364416-16364435 MS.gene033069:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 16361683 16365169 16361683 ID=MS.gene033069
chr8.2 mRNA 16361683 16365169 16361683 ID=MS.gene033069.t1;Parent=MS.gene033069
chr8.2 exon 16361683 16362832 16361683 ID=MS.gene033069.t1.exon1;Parent=MS.gene033069.t1
chr8.2 CDS 16361683 16362832 16361683 ID=cds.MS.gene033069.t1;Parent=MS.gene033069.t1
chr8.2 exon 16362953 16363134 16362953 ID=MS.gene033069.t1.exon2;Parent=MS.gene033069.t1
chr8.2 CDS 16362953 16363134 16362953 ID=cds.MS.gene033069.t1;Parent=MS.gene033069.t1
chr8.2 exon 16363512 16364146 16363512 ID=MS.gene033069.t1.exon3;Parent=MS.gene033069.t1
chr8.2 CDS 16363512 16364146 16363512 ID=cds.MS.gene033069.t1;Parent=MS.gene033069.t1
chr8.2 exon 16364283 16364760 16364283 ID=MS.gene033069.t1.exon4;Parent=MS.gene033069.t1
chr8.2 CDS 16364283 16364760 16364283 ID=cds.MS.gene033069.t1;Parent=MS.gene033069.t1
chr8.2 exon 16364876 16365169 16364876 ID=MS.gene033069.t1.exon5;Parent=MS.gene033069.t1
chr8.2 CDS 16364876 16365169 16364876 ID=cds.MS.gene033069.t1;Parent=MS.gene033069.t1
Gene Sequence

>MS.gene033069

ATGAATCCAGAGAAATTTACTCACAAAACAAACGAAGCACTTGCTGGTGCACACGAGCTAGCAATGACTTCAGGTCATGCACAAATCACTCCCTTACACCTTGCATCAATTCTAGTTTCTGATCCTAACGGTATTTTCTTCCAAGCAATTTCAAACGTTGCTGGTGAAGAATCAGCACGTGCTGTTGAACGTGTTTTGAAACAAGCATTAAAGAAACTTCCATCTCAATCACCTCCACCAGATGAAGTGCCTGGTAGCACCGCGCTTATCAAAGCGATTCGTAGAGCACAAGCTGCACAGAAATCACGTGGTGATACTCATTTAGCTGTTGATCAGTTAATCTTGGGAATTCTCGAGGATTCTCAGATTGGTGATTTGTTTAAAGAAGCCGGTGTTGCTGTTTCTAGGGTTAAAACTGAGGTTGAAAAGCTGAGGGGTAAAGATGGGAAAAAGGTTGAAAGTGCTTCTGGTGATACGAATTTTCAAGCATTGAAGACTTATGGAAGAGACTTGGTTGAACAAGCAGGGAAGCTTGATCCTGTTATTGGACGCGACGAAGAGATTCGAAGAGTTGTGAGGATTTTGTCAAGAAGGACTAAGAACAATCCTGTTCTTATTGGTGAACCTGGTGTTGGAAAAACCGCGGTTGTTGAAGGGTTGGCTCAGAGGATTGTTAGAGGTGATGTACCTAGCAATCTTGCTGATGTTAGGTTAATTGCTTTGGATATGGGAGCACTGGTTGCTGGTGCGAAGTATAGGGGTGAATTTGAGGAGAGGTTGAAGGCTGTTTTGAAAGAAGTTGAAGAAGCTGAAGGGAAGGTTATTCTTTTCATTGATGAGATTCATCTTGTTCTTGGGGCTGGTAGAACAGAAGGATCGATGGATGCTGCTAATCTTTTTAAACCAATGCTTGCTCGTGGACAGCTTCGATGTATTGGTGCTACAACGCTTGAGGAGTATAGGAAGTATGTTGAAAAAGATGCAGCATTTGAAAGAAGGTTTCAACAGGTTTATGTGGCTGAACCTAGTGTTCCGGATACAATTAGCATTCTTCGTGGATTAAAAGAGAGATATGAGGGTCATCATGGTGTTCGAATTCAAGATCGTGCGATTGTAGTTGCAGCTCAATTGTCTAGTCGGTACATAACCGGTATGTTCTTTATATTCTCTCTTCATGTTTGTGTGTTTTTGTTATTTATTTTTTATGCATTGAATGTGATGATTTCACTTGTGTTTATGCTAACATGGTATGTGTTTTGTATGTGTTAAGGGCGTCATCTACCTGACAAGGCGATTGATTTAGTTGATGAGGCATGTGCTAATGTGCGAGTTCAACTTGATAGTCAGCCTGAGGAAATTGACAATCTTGAAAGGAAGAGAATGCAACTAGAAGTTGAACTTCATGCTCTAGAGAAAGAGAAGGACAAAGCTAGCAAAGCCCGTCTTGTAGATGTAAGTATGGATGTTAACTTTTTCTTTTAATCAATGATCTCTGAATTCAAATATGCCATGATAGCATTAATTTTGATGATATATTTGACAATGAAGATATATTTATTCATTGACTGATAAATACCTTATTTAGTCAAATTTTATTCATTCAAGTAGATTTAAGCAGATGTACATTAGTTTAGTTTAATTTCATTTGTTCTAAATTAACTTCATTTTTGTAATACTAAGTACATAACTATATTGTGGTGGTGCTGCAAATGTGGGATAATGGTTTAAATTGTGTATGTGGGATAATGGTTTAAATTGTGTATCAGTTTTCATTTTATATTGTGTATTTGTAGTACTTCAATGATTCTGATAGTTGATGTTATTCGCAGGTACGGAAAGAACTCGATGACTTGAGAGACAAGCTTCAACCTCTGAAGATGAAGTATAGTAAAGAGAAAGAGAGGATTGATGAGATTCGAAGGCTGAAGCAGAAACGTGAAGAGCTCCTCTTTGCACTACAGGAGGCAGAGAGGCGGTATGATCTAGCCAGAGCTGCGGACCTGCGATATGGTGCAATTGAAGAGGTGGAAACTGCAATTAAAAATCTTGAAGGTAGCACTGATGGGAACACTGATGACAACTTAATGTTGACTGAAACAGTTGGACCGGACCAAATAGCCGAGGTTGTTAGCCGATGGACTGGTATACCGGTGACTAGGCTTGGCCAAAATGAGAAAGAAAGGTTGGTTGGACTTGGTGACAGACTGCACACTAGAGTTGTGGGACAAGACCAGGCGGTTAATGCTGTTGCTGAGGCTGTATTGAGATCAAGAGCTGGTTTAGGAAGACCTCAACAACCAACTGGTTCCTTCTTATTCCTTGGTCCAACTGGTGTTGGTAAAACTGAGCTTGCAAAGGCTCTTGCAGAGCAGCTATTTGATGATGAAAATCAGTTGGTGAGAATTGACATGTCGGAGTACATGGAACAACACTCTGTATCAAGATTGATTGGTGCACCACCAGGGTAAGTTATTAGATTCGTACCTGAATATTATTTAGACTAATAGCCTAAACTAGTAGCTAGAGTTAAATATGAATTAATATACATTCTAGTATGTGGTCTATGAGAACAAGTATCTCATTTTGCATTGAATTTTCAGGTATGTTGGACATGAAGAAGGGGGTCAATTAACAGAAGCTGTAAGGAGAAGGCCATACAGTGTTGTACTTTTTGATGAAGTTGAGAAAGCACACACATCTGTATTCAACACTCTTCTACAAGTCTTGGATGATGGGAGGTTGACTGACGGACAAGGCAGAACTGTGGACTTTAGGAACACTGTGATCATCATGACCTCTAACCTTGGTGCTGAGCATCTCTTGAGTGGACTTTCAGGAAAATGTACCATGCAAGCTGCTCGCGATCGAGTGATGCAGGAAGTAAGAAGGCATTTCAGGCCAGAATTGTTGAACCGACTGGACGAAGTTGTTGTATTTGATCCTCTATCACATGAGCAATTGAGAAAGGTTGCAAGGTTGCAAATGAAGGATGTGGCTAGCCGTCTTGCTGAGAGAGGAATTGCATTGGCAGTAACAGATGCGGCTCTAGATTATATTCTTGCTGAGAGCTATGATCCAGTAAGTGTTATTATATTATAATATCATCTTAATCTTTAGGATATAGAATATTTCAGACAAAATGGTAGTGGCTAATAATACTAACTGGTTTTTGTTATTGTTGTTTCTTGTGTAGGTGTATGGTGCTAGACCAATTAGAAGGTGGCTAGAGAGGAAGGTTGTGACAGAGCTGTCTAGGATGCTCATAAGAGAAGAAATTGATGAAAATACAACGGTTTACATTGATGCTGGTCCTAAGGGAAGTGATTTAGCATACCGTGTAGAAAAGAATGGAGGGATTGTTAATGCTGAAACTGGGGTGAAGTCTGATATTCTGATTCAGATTCCAAATGGACCGAAAAGTGATGCTGTTCAGGCTGTCAAGAAAATGAGAATTGAGGAAGTTGATGATGATGAAATGGAAGAGTGA
Protein sequence

>MS.gene033069.t1

MNPEKFTHKTNEALAGAHELAMTSGHAQITPLHLASILVSDPNGIFFQAISNVAGEESARAVERVLKQALKKLPSQSPPPDEVPGSTALIKAIRRAQAAQKSRGDTHLAVDQLILGILEDSQIGDLFKEAGVAVSRVKTEVEKLRGKDGKKVESASGDTNFQALKTYGRDLVEQAGKLDPVIGRDEEIRRVVRILSRRTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPSNLADVRLIALDMGALVAGAKYRGEFEERLKAVLKEVEEAEGKVILFIDEIHLVLGAGRTEGSMDAANLFKPMLARGQLRCIGATTLEEYRKYVEKDAAFERRFQQVYVAEPSVPDTISILRGLKERYEGHHGVRIQDRAIVVAAQLSSRYITGRHLPDKAIDLVDEACANVRVQLDSQPEEIDNLERKRMQLEVELHALEKEKDKASKARLVDVRKELDDLRDKLQPLKMKYSKEKERIDEIRRLKQKREELLFALQEAERRYDLARAADLRYGAIEEVETAIKNLEGSTDGNTDDNLMLTETVGPDQIAEVVSRWTGIPVTRLGQNEKERLVGLGDRLHTRVVGQDQAVNAVAEAVLRSRAGLGRPQQPTGSFLFLGPTGVGKTELAKALAEQLFDDENQLVRIDMSEYMEQHSVSRLIGAPPGYVGHEEGGQLTEAVRRRPYSVVLFDEVEKAHTSVFNTLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGAEHLLSGLSGKCTMQAARDRVMQEVRRHFRPELLNRLDEVVVFDPLSHEQLRKVARLQMKDVASRLAERGIALAVTDAALDYILAESYDPVYGARPIRRWLERKVVTELSRMLIREEIDENTTVYIDAGPKGSDLAYRVEKNGGIVNAETGVKSDILIQIPNGPKSDAVQAVKKMRIEEVDDDEMEE