Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035233.t1 | XP_013458203.1 | 97 | 635 | 18 | 1 | 1 | 634 | 1 | 635 | 0.00E+00 | 1209.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035233.t1 | Q9NYV6 | 27.6 | 573 | 362 | 17 | 32 | 572 | 58 | 609 | 1.6e-35 | 152.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035233.t1 | A0A072UQX1 | 97.0 | 635 | 18 | 1 | 1 | 634 | 1 | 635 | 0.0e+00 | 1209.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035233 | MS.gene73391 | PPI |
| MS.gene035233 | MS.gene036585 | PPI |
| MS.gene035233 | MS.gene065392 | PPI |
| MS.gene035233 | MS.gene024279 | PPI |
| MS.gene035233 | MS.gene053492 | PPI |
| MS.gene035233 | MS.gene041467 | PPI |
| MS.gene035233 | MS.gene064140 | PPI |
| MS.gene035233 | MS.gene68633 | PPI |
| MS.gene035233 | MS.gene033712 | PPI |
| MS.gene035233 | MS.gene033714 | PPI |
| MS.gene035233 | MS.gene030166 | PPI |
| MS.gene035233 | MS.gene39636 | PPI |
| MS.gene035233 | MS.gene26516 | PPI |
| MS.gene035233 | MS.gene26777 | PPI |
| MS.gene035233 | MS.gene047813 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035233.t1 | MTR_4g117680 | 97.008 | 635 | 18 | 1 | 1 | 634 | 1 | 635 | 0.0 | 1266 |
| MS.gene035233.t1 | MTR_8g085920 | 87.342 | 79 | 10 | 0 | 193 | 271 | 1 | 79 | 9.62e-42 | 147 |
| MS.gene035233.t1 | MTR_4g117730 | 70.149 | 67 | 20 | 0 | 205 | 271 | 1 | 67 | 2.67e-19 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035233.t1 | AT2G34750 | 45.774 | 627 | 296 | 12 | 17 | 633 | 21 | 613 | 3.90e-174 | 509 |
| MS.gene035233.t1 | AT1G30590 | 45.513 | 624 | 300 | 13 | 17 | 634 | 17 | 606 | 5.51e-169 | 496 |
| MS.gene035233.t1 | AT1G30590 | 45.513 | 624 | 300 | 13 | 17 | 634 | 17 | 606 | 5.51e-169 | 496 |
| MS.gene035233.t1 | AT1G30590 | 45.513 | 624 | 297 | 14 | 17 | 634 | 17 | 603 | 7.88e-167 | 490 |
| MS.gene035233.t1 | AT2G39240 | 40.126 | 633 | 288 | 15 | 17 | 629 | 7 | 568 | 6.47e-126 | 384 |
| MS.gene035233.t1 | AT2G39240 | 40.126 | 633 | 288 | 15 | 17 | 629 | 7 | 568 | 6.47e-126 | 384 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTATGAAATATGGGTTTC+AGG | 0.216795 | 8.1:+9603177 | MS.gene035233:CDS |
| ACGCCCAAATCTATGAAATA+TGG | 0.220265 | 8.1:+9603169 | MS.gene035233:CDS |
| AGAAATTCAAAGGCGTATTT+AGG | 0.222570 | 8.1:-9596830 | None:intergenic |
| AAACCCATATTTCATAGATT+TGG | 0.238800 | 8.1:-9603173 | None:intergenic |
| CAACGAAGATTTATCTGTTA+TGG | 0.271233 | 8.1:+9595879 | MS.gene035233:CDS |
| TCCTGGTTGTAGGTGGAAAT+TGG | 0.276044 | 8.1:+9597678 | MS.gene035233:intron |
| AACCCATATTTCATAGATTT+GGG | 0.308026 | 8.1:-9603172 | None:intergenic |
| GGCTCCTTAGCGAAACGTTT+TGG | 0.310138 | 8.1:-9596980 | None:intergenic |
| TTCAACAAACATGGATCAAA+TGG | 0.328326 | 8.1:-9602788 | None:intergenic |
| AAATGCCCATGGAGCTAATC+TGG | 0.332385 | 8.1:+9602406 | MS.gene035233:CDS |
| ATGCTTGAGAGCGGTGAAAT+TGG | 0.343084 | 8.1:+9597521 | MS.gene035233:CDS |
| GTACCACAGAGTTGTTAAAT+AGG | 0.350184 | 8.1:+9601019 | MS.gene035233:CDS |
| TCACGACGCTGCTAAACTTT+TGG | 0.352317 | 8.1:+9596268 | MS.gene035233:CDS |
| GCGGATCTTTCCAAGGCTTT+CGG | 0.378275 | 8.1:+9602737 | MS.gene035233:CDS |
| TGGAGTTGACATGAAGAATA+AGG | 0.387882 | 8.1:+9596865 | MS.gene035233:CDS |
| GGATATGAATGATTATGATA+TGG | 0.391946 | 8.1:+9603090 | MS.gene035233:CDS |
| TCTGAAACACATAGCTGATT+TGG | 0.393971 | 8.1:+9596910 | MS.gene035233:CDS |
| TAAATTGTTCGTTACCTTCA+AGG | 0.395606 | 8.1:-9602445 | None:intergenic |
| GGCATACTGTGGACAACTAT+TGG | 0.407503 | 8.1:-9596959 | None:intergenic |
| AAGCGGCATTACCTAGTAAT+AGG | 0.412363 | 8.1:-9601522 | None:intergenic |
| AGTAGAGGAATTTCTAAAAC+AGG | 0.414435 | 8.1:+9602655 | MS.gene035233:CDS |
| CGCCCAAATCTATGAAATAT+GGG | 0.416002 | 8.1:+9603170 | MS.gene035233:CDS |
| AAAATTTACACCACATCCTT+CGG | 0.424276 | 8.1:-9601457 | None:intergenic |
| TGATGAGAAATATTATAGTA+TGG | 0.433744 | 8.1:+9597779 | MS.gene035233:CDS |
| GAACAATTTGCAAGGCAATC+AGG | 0.437527 | 8.1:+9601041 | MS.gene035233:CDS |
| GCTTGAGCTCATCGTGTCAT+TGG | 0.439526 | 8.1:+9596645 | MS.gene035233:CDS |
| CTTTCGCATGAGATCATTGA+TGG | 0.440574 | 8.1:+9602353 | MS.gene035233:CDS |
| ATAAGCTTCACTTTAAGTCT+AGG | 0.441192 | 8.1:-9602381 | None:intergenic |
| TGTTTCACAGGCTGCTTCAA+AGG | 0.445012 | 8.1:+9596757 | MS.gene035233:intron |
| AGCTTTCGAGATTGAGCTTG+TGG | 0.456347 | 8.1:+9596569 | MS.gene035233:intron |
| GGGATTATGCTCCAACTGAT+AGG | 0.461972 | 8.1:+9596590 | MS.gene035233:CDS |
| GAAGTTTCTTTCATCTGAGT+TGG | 0.464213 | 8.1:+9601839 | MS.gene035233:CDS |
| GTGTTAATCCTCCTATTACT+AGG | 0.469232 | 8.1:+9601511 | MS.gene035233:CDS |
| GTTTCACAGGCTGCTTCAAA+GGG | 0.472233 | 8.1:+9596758 | MS.gene035233:intron |
| AACGAGGAGCTCAGTGATAG+TGG | 0.472727 | 8.1:+9603025 | MS.gene035233:CDS |
| TGGATGGGATGGCAAGATAC+AGG | 0.478954 | 8.1:+9597698 | MS.gene035233:CDS |
| TGACCATCACCAGCTACTTA+AGG | 0.479692 | 8.1:+9602951 | MS.gene035233:intron |
| ATTTATCCCACCGAAAGCCT+TGG | 0.486236 | 8.1:-9602747 | None:intergenic |
| AGAAATTCCTCTACTACGCT+AGG | 0.486325 | 8.1:-9602647 | None:intergenic |
| GCTTTCGAGATTGAGCTTGT+GGG | 0.486729 | 8.1:+9596570 | MS.gene035233:intron |
| ATGTTTCCAGATTAGCTCCA+TGG | 0.488983 | 8.1:-9602412 | None:intergenic |
| GATAAATCTTCGTTGACAAT+CGG | 0.489575 | 8.1:-9595871 | None:intergenic |
| AGATACCCTTCTTGCAGTCT+CGG | 0.490560 | 8.1:+9595948 | MS.gene035233:CDS |
| GATCTTTCCAAGGCTTTCGG+TGG | 0.493557 | 8.1:+9602740 | MS.gene035233:CDS |
| CTCCATTGCAAATATGCACT+TGG | 0.495884 | 8.1:-9597727 | None:intergenic |
| AAATTGTTCGTTACCTTCAA+GGG | 0.496820 | 8.1:-9602444 | None:intergenic |
| GGTGAAATTGGAGAAATTGT+TGG | 0.498711 | 8.1:+9597533 | MS.gene035233:CDS |
| GTGATAATCGCAAGGGAGCA+AGG | 0.499704 | 8.1:-9596937 | None:intergenic |
| GAAGCGATTCATGCTGGATA+GGG | 0.499853 | 8.1:-9596446 | None:intergenic |
| ATGAATGATTATGATATGGC+CGG | 0.501292 | 8.1:+9603094 | MS.gene035233:CDS |
| AGAAGCGATTCATGCTGGAT+AGG | 0.502609 | 8.1:-9596447 | None:intergenic |
| GAAGAATAAGGTTCTGCCTC+GGG | 0.505488 | 8.1:+9596877 | MS.gene035233:CDS |
| ACATAAATTGAATCCCTTGA+AGG | 0.505777 | 8.1:+9602431 | MS.gene035233:CDS |
| GCATCATATTTCCTATCAGT+TGG | 0.506860 | 8.1:-9596601 | None:intergenic |
| TTTCATAGATTTGGGCGTGA+TGG | 0.514294 | 8.1:-9603164 | None:intergenic |
| TGTTTCCAGATTAGCTCCAT+GGG | 0.520405 | 8.1:-9602411 | None:intergenic |
| AAGAAAGAAGCGATTCATGC+TGG | 0.521142 | 8.1:-9596452 | None:intergenic |
| ATCAACTTATCCACAAGTGC+AGG | 0.525390 | 8.1:-9597569 | None:intergenic |
| GCTCGAGGCGGATCTTTCCA+AGG | 0.526827 | 8.1:+9602730 | MS.gene035233:CDS |
| ATATGATGCAGCTGATATCA+TGG | 0.528281 | 8.1:+9596615 | MS.gene035233:CDS |
| AGATTTATCTGTTATGGAGG+AGG | 0.529140 | 8.1:+9595885 | MS.gene035233:CDS |
| GGTTGTAGGTGGAAATTGGA+TGG | 0.531254 | 8.1:+9597682 | MS.gene035233:intron |
| TAGGTGGAAATTGGATGGGA+TGG | 0.531448 | 8.1:+9597687 | MS.gene035233:intron |
| TCAACAAACATGGATCAAAT+GGG | 0.531713 | 8.1:-9602787 | None:intergenic |
| ATATGGGTTTCAGGCACAGA+TGG | 0.533433 | 8.1:+9603186 | MS.gene035233:CDS |
| TACAATGCTACCTGCACTTG+TGG | 0.534558 | 8.1:+9597559 | MS.gene035233:CDS |
| ATCTTTCCAAGGCTTTCGGT+GGG | 0.536074 | 8.1:+9602741 | MS.gene035233:CDS |
| AATTCAAAGGCGTATTTAGG+AGG | 0.538124 | 8.1:-9596827 | None:intergenic |
| ATTCTTGTGGTTCAGGATGG+TGG | 0.540191 | 8.1:+9596191 | MS.gene035233:intron |
| TGTGGATAAGTTGATAGAGT+TGG | 0.541891 | 8.1:+9597577 | MS.gene035233:CDS |
| GGAATTTCTAAAACAGGCAA+AGG | 0.549923 | 8.1:+9602661 | MS.gene035233:CDS |
| TAAAACAGGCAAAGGCTGCT+CGG | 0.551011 | 8.1:+9602669 | MS.gene035233:CDS |
| CTTGACCAGCGGACAAAATG+TGG | 0.555293 | 8.1:-9602974 | None:intergenic |
| TCTTGAATGCTGTCAAAGCA+AGG | 0.555849 | 8.1:+9601107 | MS.gene035233:CDS |
| GGTTTCAGGCACAGATGGAA+GGG | 0.558054 | 8.1:+9603191 | MS.gene035233:CDS |
| GGGTTTCAGGCACAGATGGA+AGG | 0.558736 | 8.1:+9603190 | MS.gene035233:CDS |
| TGAAGAATAAGGTTCTGCCT+CGG | 0.559544 | 8.1:+9596876 | MS.gene035233:CDS |
| CTGTCAAAGCAAGGGCCGAT+TGG | 0.561772 | 8.1:+9601116 | MS.gene035233:CDS |
| AAGCAAGGGCCGATTGGCTG+AGG | 0.562906 | 8.1:+9601122 | MS.gene035233:CDS |
| TTGGGAATACCTCAGCCAAT+CGG | 0.566052 | 8.1:-9601131 | None:intergenic |
| CGAAGATTTATCTGTTATGG+AGG | 0.567769 | 8.1:+9595882 | MS.gene035233:CDS |
| TTAAATAGGAACAATTTGCA+AGG | 0.569913 | 8.1:+9601033 | MS.gene035233:CDS |
| AACTATTGGTGATAATCGCA+AGG | 0.570156 | 8.1:-9596945 | None:intergenic |
| TGCCAAGTGCATATTTGCAA+TGG | 0.570882 | 8.1:+9597725 | MS.gene035233:CDS |
| AAGACCCGCTTCAACACTCC+CGG | 0.576390 | 8.1:-9603113 | None:intergenic |
| GATGTTCTATGCATGCGCAT+TGG | 0.580066 | 8.1:+9601431 | MS.gene035233:CDS |
| TGATAATCGCAAGGGAGCAA+GGG | 0.586042 | 8.1:-9596936 | None:intergenic |
| TGAATGATTATGATATGGCC+GGG | 0.586844 | 8.1:+9603095 | MS.gene035233:CDS |
| ATGCTGGATAGGGTCAATGT+AGG | 0.592083 | 8.1:-9596436 | None:intergenic |
| CGGCATTACCTAGTAATAGG+AGG | 0.597341 | 8.1:-9601519 | None:intergenic |
| ACTATCACTGAGCTCCTCGT+TGG | 0.597389 | 8.1:-9603023 | None:intergenic |
| ATGTTTGCCTAGCGTAGTAG+AGG | 0.599342 | 8.1:+9602640 | MS.gene035233:CDS |
| GCTTACCGAGACTGCAAGAA+GGG | 0.601568 | 8.1:-9595953 | None:intergenic |
| GTTGTAGGTGGAAATTGGAT+GGG | 0.602234 | 8.1:+9597683 | MS.gene035233:intron |
| ATGGCCGGGAGTGTTGAAGC+GGG | 0.607938 | 8.1:+9603109 | MS.gene035233:CDS |
| GAAGCTTATCAAAATGCCCA+TGG | 0.616331 | 8.1:+9602395 | MS.gene035233:CDS |
| GGCTTACCGAGACTGCAAGA+AGG | 0.617000 | 8.1:-9595954 | None:intergenic |
| TATGCCAAAACGTTTCGCTA+AGG | 0.621248 | 8.1:+9596976 | MS.gene035233:CDS |
| TTCAGAGCAGCATGCACCCG+AGG | 0.627619 | 8.1:-9596893 | None:intergenic |
| ATACAAGTCAAAATTTGCCC+AGG | 0.628013 | 8.1:+9601304 | MS.gene035233:CDS |
| GAAATTGGAGAAATTGTTGG+TGG | 0.630468 | 8.1:+9597536 | MS.gene035233:CDS |
| GATAATCGCAAGGGAGCAAG+GGG | 0.631795 | 8.1:-9596935 | None:intergenic |
| CTTGAATGCTGTCAAAGCAA+GGG | 0.633580 | 8.1:+9601108 | MS.gene035233:CDS |
| TCGCATGAGATCATTGATGG+AGG | 0.634684 | 8.1:+9602356 | MS.gene035233:CDS |
| GTTCCTATTTAACAACTCTG+TGG | 0.638060 | 8.1:-9601022 | None:intergenic |
| GTTTGTTGAAGAAAAGTGAA+AGG | 0.640363 | 8.1:+9602801 | MS.gene035233:CDS |
| TATGGCCGGGAGTGTTGAAG+CGG | 0.646112 | 8.1:+9603108 | MS.gene035233:CDS |
| GCATGCGCATTGGATTCCGA+AGG | 0.647857 | 8.1:+9601441 | MS.gene035233:CDS |
| TGGTTGCTAACATCATACAA+AGG | 0.652099 | 8.1:+9601859 | MS.gene035233:CDS |
| GCATTGGATTCCGAAGGATG+TGG | 0.661172 | 8.1:+9601447 | MS.gene035233:CDS |
| AAACATCTCTGTGAGGACCA+TGG | 0.662377 | 8.1:-9601481 | None:intergenic |
| ACTATTGGTGATAATCGCAA+GGG | 0.679273 | 8.1:-9596944 | None:intergenic |
| TTTCGATGATTTGCTCGAGG+CGG | 0.688171 | 8.1:+9602718 | MS.gene035233:CDS |
| ATGGGATGGCAAGATACAGG+AGG | 0.711153 | 8.1:+9597701 | MS.gene035233:CDS |
| ATGAATCAAACATCTCTGTG+AGG | 0.746973 | 8.1:-9601488 | None:intergenic |
| AATATGTTAATGCTTGAGAG+CGG | 0.775120 | 8.1:+9597512 | MS.gene035233:CDS |
| ATATGATACATATGCCAACG+AGG | 0.781154 | 8.1:+9603009 | MS.gene035233:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 9595856 | 9603264 | 9595856 | ID=MS.gene035233 |
| chr8.1 | mRNA | 9595856 | 9603264 | 9595856 | ID=MS.gene035233.t1;Parent=MS.gene035233 |
| chr8.1 | exon | 9595856 | 9595969 | 9595856 | ID=MS.gene035233.t1.exon1;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9595856 | 9595969 | 9595856 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9596206 | 9596289 | 9596206 | ID=MS.gene035233.t1.exon2;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9596206 | 9596289 | 9596206 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9596405 | 9596476 | 9596405 | ID=MS.gene035233.t1.exon3;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9596405 | 9596476 | 9596405 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9596571 | 9596666 | 9596571 | ID=MS.gene035233.t1.exon4;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9596571 | 9596666 | 9596571 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9596767 | 9597003 | 9596767 | ID=MS.gene035233.t1.exon5;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9596767 | 9597003 | 9596767 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9597491 | 9597601 | 9597491 | ID=MS.gene035233.t1.exon6;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9597491 | 9597601 | 9597491 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9597690 | 9597800 | 9597690 | ID=MS.gene035233.t1.exon7;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9597690 | 9597800 | 9597690 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9601018 | 9601143 | 9601018 | ID=MS.gene035233.t1.exon8;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9601018 | 9601143 | 9601018 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9601257 | 9601325 | 9601257 | ID=MS.gene035233.t1.exon9;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9601257 | 9601325 | 9601257 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9601426 | 9601532 | 9601426 | ID=MS.gene035233.t1.exon10;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9601426 | 9601532 | 9601426 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9601797 | 9601880 | 9601797 | ID=MS.gene035233.t1.exon11;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9601797 | 9601880 | 9601797 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9601991 | 9602078 | 9601991 | ID=MS.gene035233.t1.exon12;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9601991 | 9602078 | 9601991 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9602333 | 9602452 | 9602333 | ID=MS.gene035233.t1.exon13;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9602333 | 9602452 | 9602333 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9602638 | 9602822 | 9602638 | ID=MS.gene035233.t1.exon14;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9602638 | 9602822 | 9602638 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| chr8.1 | exon | 9602964 | 9603264 | 9602964 | ID=MS.gene035233.t1.exon15;Parent=MS.gene035233.t1 |
| chr8.1 | CDS | 9602964 | 9603264 | 9602964 | ID=cds.MS.gene035233.t1;Parent=MS.gene035233.t1 |
| Gene Sequence |
| Protein sequence |
