Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036585.t1 | XP_013458203.1 | 96.7 | 636 | 19 | 2 | 1 | 635 | 1 | 635 | 0.00E+00 | 1203.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036585.t1 | Q9NYV6 | 27.4 | 574 | 363 | 17 | 32 | 573 | 58 | 609 | 4.1e-36 | 154.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036585.t1 | A0A072UQX1 | 96.7 | 636 | 19 | 2 | 1 | 635 | 1 | 635 | 0.0e+00 | 1203.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035233 | MS.gene036585 | PPI |
| MS.gene033712 | MS.gene036585 | PPI |
| MS.gene036585 | MS.gene047813 | PPI |
| MS.gene26777 | MS.gene036585 | PPI |
| MS.gene036585 | MS.gene024279 | PPI |
| MS.gene73391 | MS.gene036585 | PPI |
| MS.gene036585 | MS.gene041467 | PPI |
| MS.gene036585 | MS.gene064140 | PPI |
| MS.gene036585 | MS.gene030166 | PPI |
| MS.gene036585 | MS.gene68633 | PPI |
| MS.gene053492 | MS.gene036585 | PPI |
| MS.gene033714 | MS.gene036585 | PPI |
| MS.gene26516 | MS.gene036585 | PPI |
| MS.gene036585 | MS.gene39636 | PPI |
| MS.gene065392 | MS.gene036585 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036585.t1 | MTR_4g117680 | 96.698 | 636 | 19 | 2 | 1 | 635 | 1 | 635 | 0.0 | 1259 |
| MS.gene036585.t1 | MTR_8g085920 | 84.810 | 79 | 12 | 0 | 193 | 271 | 1 | 79 | 1.29e-40 | 144 |
| MS.gene036585.t1 | MTR_4g117730 | 68.182 | 66 | 21 | 0 | 205 | 270 | 1 | 66 | 4.42e-18 | 79.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene036585.t1 | AT2G34750 | 45.527 | 626 | 300 | 11 | 17 | 634 | 21 | 613 | 4.26e-174 | 509 |
| MS.gene036585.t1 | AT1G30590 | 45.440 | 625 | 300 | 13 | 17 | 635 | 17 | 606 | 3.25e-169 | 496 |
| MS.gene036585.t1 | AT1G30590 | 45.440 | 625 | 300 | 13 | 17 | 635 | 17 | 606 | 3.25e-169 | 496 |
| MS.gene036585.t1 | AT1G30590 | 45.440 | 625 | 297 | 14 | 17 | 635 | 17 | 603 | 3.46e-167 | 491 |
| MS.gene036585.t1 | AT2G39240 | 40.063 | 634 | 288 | 15 | 17 | 630 | 7 | 568 | 1.05e-125 | 383 |
| MS.gene036585.t1 | AT2G39240 | 40.063 | 634 | 288 | 15 | 17 | 630 | 7 | 568 | 1.05e-125 | 383 |
Find 114 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTATGAAATATGGGTTTC+AGG | 0.216795 | 8.3:+8491142 | MS.gene036585:CDS |
| ACGCCCAAATCTATGAAATA+TGG | 0.220265 | 8.3:+8491134 | MS.gene036585:CDS |
| AGAAATTCAAAGGCGTATTT+AGG | 0.222570 | 8.3:-8484797 | None:intergenic |
| AAACCCATATTTCATAGATT+TGG | 0.238800 | 8.3:-8491138 | None:intergenic |
| CAACGAAGATTTATCTGTTA+TGG | 0.271233 | 8.3:+8483845 | MS.gene036585:CDS |
| TCCTGGTTGTAGGTGGAAAT+TGG | 0.276044 | 8.3:+8485664 | MS.gene036585:intron |
| AACCCATATTTCATAGATTT+GGG | 0.308026 | 8.3:-8491137 | None:intergenic |
| GGCTCCTTAGCGAAACGTTT+TGG | 0.310138 | 8.3:-8484947 | None:intergenic |
| TTCAACAAACATGGATCAAA+TGG | 0.328326 | 8.3:-8490753 | None:intergenic |
| AAATGCCCATGGAGCTAATC+TGG | 0.332385 | 8.3:+8490371 | MS.gene036585:CDS |
| ATGCTTGAGAGCGGTGAAAT+TGG | 0.343084 | 8.3:+8485507 | MS.gene036585:CDS |
| GTACCACAGAGTTGTTAAAT+AGG | 0.350184 | 8.3:+8488983 | MS.gene036585:CDS |
| TCACGACGCTGCTAAACTTT+TGG | 0.352317 | 8.3:+8484236 | MS.gene036585:CDS |
| GGCATACTGTGGACAACTAT+AGG | 0.373269 | 8.3:-8484926 | None:intergenic |
| GCGGATCTTTCCAAGGCTTT+CGG | 0.378275 | 8.3:+8490702 | MS.gene036585:CDS |
| TGGAGTTGACATGAAGAATA+AGG | 0.387882 | 8.3:+8484832 | MS.gene036585:CDS |
| TAAATTGTTTGTTACCTTCA+AGG | 0.387978 | 8.3:-8490410 | None:intergenic |
| GGATATGAATGATTATGATA+TGG | 0.391946 | 8.3:+8491055 | MS.gene036585:CDS |
| TCTGAAACACATAGCTGATT+TGG | 0.393971 | 8.3:+8484877 | MS.gene036585:CDS |
| AAGCGGCATTACCTAGTAAT+AGG | 0.412363 | 8.3:-8489486 | None:intergenic |
| AGTAGAGGAATTTCTAAAAC+AGG | 0.414435 | 8.3:+8490620 | MS.gene036585:CDS |
| CGCCCAAATCTATGAAATAT+GGG | 0.416002 | 8.3:+8491135 | MS.gene036585:CDS |
| AAAATTTACACCACATCCTT+CGG | 0.424276 | 8.3:-8489421 | None:intergenic |
| GAACAATTTGCAAGGCAATC+AGG | 0.437527 | 8.3:+8489005 | MS.gene036585:CDS |
| GCTTGAGCTCATCGTGTCAT+TGG | 0.439526 | 8.3:+8484613 | MS.gene036585:CDS |
| CTTTCGCATGAGATCATTGA+TGG | 0.440574 | 8.3:+8490318 | MS.gene036585:CDS |
| ATAAGCTTCACTTTAAGTCT+AGG | 0.441192 | 8.3:-8490346 | None:intergenic |
| AAATTGTTTGTTACCTTCAA+GGG | 0.451283 | 8.3:-8490409 | None:intergenic |
| TGACCATCTCCAGCTACTTA+AGG | 0.454991 | 8.3:+8490916 | MS.gene036585:intron |
| AGCTTTCGAGATTGAGCTTG+TGG | 0.456347 | 8.3:+8484537 | MS.gene036585:intron |
| GGGATTATGCTCCAACTGAT+AGG | 0.461972 | 8.3:+8484558 | MS.gene036585:CDS |
| GAAGTTTCTTTCATCTGAGT+TGG | 0.464213 | 8.3:+8489803 | MS.gene036585:CDS |
| GTGTTAATCCTCCTATTACT+AGG | 0.469232 | 8.3:+8489475 | MS.gene036585:CDS |
| AACGAGGAGCTCAGTGATAG+TGG | 0.472727 | 8.3:+8490990 | MS.gene036585:CDS |
| TGGATGGGATGGCAAGATAC+AGG | 0.478954 | 8.3:+8485684 | MS.gene036585:CDS |
| ATTTATCCCACCGAAAGCCT+TGG | 0.486236 | 8.3:-8490712 | None:intergenic |
| AGAAATTCCTCTACTACGCT+AGG | 0.486325 | 8.3:-8490612 | None:intergenic |
| GCTTTCGAGATTGAGCTTGT+GGG | 0.486729 | 8.3:+8484538 | MS.gene036585:intron |
| ATGTTTCCAGATTAGCTCCA+TGG | 0.488983 | 8.3:-8490377 | None:intergenic |
| GATAAATCTTCGTTGACAAT+CGG | 0.489575 | 8.3:-8483837 | None:intergenic |
| AGATACCCTTCTTGCAGTCT+CGG | 0.490560 | 8.3:+8483914 | MS.gene036585:CDS |
| GTGATAATCGCAAGGGAGCA+AGG | 0.492980 | 8.3:-8484904 | None:intergenic |
| GATCTTTCCAAGGCTTTCGG+TGG | 0.493557 | 8.3:+8490705 | MS.gene036585:CDS |
| CTCCATTGCAAATATGCACT+TGG | 0.495884 | 8.3:-8485713 | None:intergenic |
| GGTGAAATTGGAGAAATTGT+TGG | 0.498711 | 8.3:+8485519 | MS.gene036585:CDS |
| GAAGCGATTCATGCTGGATA+GGG | 0.499853 | 8.3:-8484414 | None:intergenic |
| ATGAATGATTATGATATGGC+CGG | 0.501292 | 8.3:+8491059 | MS.gene036585:CDS |
| AGAAGCGATTCATGCTGGAT+AGG | 0.502609 | 8.3:-8484415 | None:intergenic |
| GAAGAATAAGGTTCTGCCTC+GGG | 0.505488 | 8.3:+8484844 | MS.gene036585:CDS |
| ACATAAATTGAATCCCTTGA+AGG | 0.505777 | 8.3:+8490396 | MS.gene036585:CDS |
| GCATCATATTTCCTATCAGT+TGG | 0.506860 | 8.3:-8484569 | None:intergenic |
| ATTCTCGTGGTTCAGGATGG+TGG | 0.508749 | 8.3:+8484159 | MS.gene036585:intron |
| TTTCATAGATTTGGGCGTGA+TGG | 0.514294 | 8.3:-8491129 | None:intergenic |
| TGTTTCCAGATTAGCTCCAT+GGG | 0.520405 | 8.3:-8490376 | None:intergenic |
| AAGAAAGAAGCGATTCATGC+TGG | 0.521142 | 8.3:-8484420 | None:intergenic |
| ATCAACTTATCCACAAGTGC+AGG | 0.525390 | 8.3:-8485555 | None:intergenic |
| GCTCGAGGCGGATCTTTCCA+AGG | 0.526827 | 8.3:+8490695 | MS.gene036585:CDS |
| ATATGATGCAGCTGATATCA+TGG | 0.528281 | 8.3:+8484583 | MS.gene036585:CDS |
| AGATTTATCTGTTATGGAGG+AGG | 0.529140 | 8.3:+8483851 | MS.gene036585:CDS |
| GGTTGTAGGTGGAAATTGGA+TGG | 0.531254 | 8.3:+8485668 | MS.gene036585:intron |
| TAGGTGGAAATTGGATGGGA+TGG | 0.531448 | 8.3:+8485673 | MS.gene036585:intron |
| TCAACAAACATGGATCAAAT+GGG | 0.531713 | 8.3:-8490752 | None:intergenic |
| ATATGGGTTTCAGGCACAGA+TGG | 0.533433 | 8.3:+8491151 | MS.gene036585:CDS |
| TACAATGCTACCTGCACTTG+TGG | 0.534558 | 8.3:+8485545 | MS.gene036585:CDS |
| ATCTTTCCAAGGCTTTCGGT+GGG | 0.536074 | 8.3:+8490706 | MS.gene036585:CDS |
| AATTCAAAGGCGTATTTAGG+AGG | 0.538124 | 8.3:-8484794 | None:intergenic |
| TGTGGATAAGTTGATAGAGT+TGG | 0.541891 | 8.3:+8485563 | MS.gene036585:CDS |
| TGATAATCGCAAGGGAGCAA+GGG | 0.543178 | 8.3:-8484903 | None:intergenic |
| GGAATTTCTAAAACAGGCAA+AGG | 0.549923 | 8.3:+8490626 | MS.gene036585:CDS |
| TAAAACAGGCAAAGGCTGCT+CGG | 0.551011 | 8.3:+8490634 | MS.gene036585:CDS |
| CTTGACCAGCGGACAAAATG+TGG | 0.555293 | 8.3:-8490939 | None:intergenic |
| TCTTGAATGCTGTCAAAGCA+AGG | 0.555849 | 8.3:+8489071 | MS.gene036585:CDS |
| AACTATAGGTGATAATCGCA+AGG | 0.557758 | 8.3:-8484912 | None:intergenic |
| GGTTTCAGGCACAGATGGAA+GGG | 0.558054 | 8.3:+8491156 | MS.gene036585:CDS |
| GGGTTTCAGGCACAGATGGA+AGG | 0.558736 | 8.3:+8491155 | MS.gene036585:CDS |
| TGAAGAATAAGGTTCTGCCT+CGG | 0.559544 | 8.3:+8484843 | MS.gene036585:CDS |
| CTGTCAAAGCAAGGGCCGAT+TGG | 0.561772 | 8.3:+8489080 | MS.gene036585:CDS |
| AAGCAAGGGCCGATTGGCTG+AGG | 0.562906 | 8.3:+8489086 | MS.gene036585:CDS |
| TTGGGAATACCTCAGCCAAT+CGG | 0.566052 | 8.3:-8489095 | None:intergenic |
| CGAAGATTTATCTGTTATGG+AGG | 0.567769 | 8.3:+8483848 | MS.gene036585:CDS |
| TTAAATAGGAACAATTTGCA+AGG | 0.569913 | 8.3:+8488997 | MS.gene036585:CDS |
| TGCCAAGTGCATATTTGCAA+TGG | 0.570882 | 8.3:+8485711 | MS.gene036585:CDS |
| AAGACCCGCTTCAACACTCC+CGG | 0.576390 | 8.3:-8491078 | None:intergenic |
| GATGTTCTATGCATGCGCAT+TGG | 0.580066 | 8.3:+8489395 | MS.gene036585:CDS |
| TGAATGATTATGATATGGCC+GGG | 0.586844 | 8.3:+8491060 | MS.gene036585:CDS |
| ATGCTGGATAGGGTCAATGT+AGG | 0.592083 | 8.3:-8484404 | None:intergenic |
| CGGCATTACCTAGTAATAGG+AGG | 0.597341 | 8.3:-8489483 | None:intergenic |
| ACTATCACTGAGCTCCTCGT+TGG | 0.597389 | 8.3:-8490988 | None:intergenic |
| ATGTTTGCCTAGCGTAGTAG+AGG | 0.599342 | 8.3:+8490605 | MS.gene036585:CDS |
| GCTTACCGAGACTGCAAGAA+GGG | 0.601568 | 8.3:-8483919 | None:intergenic |
| GTTGTAGGTGGAAATTGGAT+GGG | 0.602234 | 8.3:+8485669 | MS.gene036585:intron |
| ATGGCCGGGAGTGTTGAAGC+GGG | 0.607938 | 8.3:+8491074 | MS.gene036585:CDS |
| GAAGCTTATCAAAATGCCCA+TGG | 0.616331 | 8.3:+8490360 | MS.gene036585:CDS |
| GGCTTACCGAGACTGCAAGA+AGG | 0.617000 | 8.3:-8483920 | None:intergenic |
| TATGCCAAAACGTTTCGCTA+AGG | 0.621248 | 8.3:+8484943 | MS.gene036585:CDS |
| TTCAGAGCAGCATGCACCCG+AGG | 0.627619 | 8.3:-8484860 | None:intergenic |
| ATACAAGTCAAAATTTGCCC+AGG | 0.628013 | 8.3:+8489268 | MS.gene036585:CDS |
| GAAATTGGAGAAATTGTTGG+TGG | 0.630468 | 8.3:+8485522 | MS.gene036585:CDS |
| GATAATCGCAAGGGAGCAAG+GGG | 0.631795 | 8.3:-8484902 | None:intergenic |
| CTTGAATGCTGTCAAAGCAA+GGG | 0.633580 | 8.3:+8489072 | MS.gene036585:CDS |
| TCGCATGAGATCATTGATGG+AGG | 0.634684 | 8.3:+8490321 | MS.gene036585:CDS |
| GTTCCTATTTAACAACTCTG+TGG | 0.638060 | 8.3:-8488986 | None:intergenic |
| GTTTGTTGAAGAAAAGTGAA+AGG | 0.640363 | 8.3:+8490766 | MS.gene036585:CDS |
| TATGGCCGGGAGTGTTGAAG+CGG | 0.646112 | 8.3:+8491073 | MS.gene036585:CDS |
| GCATGCGCATTGGATTCCGA+AGG | 0.647857 | 8.3:+8489405 | MS.gene036585:CDS |
| TGGTTGCTAACATCATACAA+AGG | 0.652099 | 8.3:+8489823 | MS.gene036585:CDS |
| GCATTGGATTCCGAAGGATG+TGG | 0.661172 | 8.3:+8489411 | MS.gene036585:CDS |
| AAACATCTCTGTGAGGACCA+TGG | 0.662377 | 8.3:-8489445 | None:intergenic |
| TTTCGATGATTTGCTCGAGG+CGG | 0.688171 | 8.3:+8490683 | MS.gene036585:CDS |
| ACTATAGGTGATAATCGCAA+GGG | 0.699606 | 8.3:-8484911 | None:intergenic |
| ATGGGATGGCAAGATACAGG+AGG | 0.711153 | 8.3:+8485687 | MS.gene036585:CDS |
| ATGAATCAAACATCTCTGTG+AGG | 0.746973 | 8.3:-8489452 | None:intergenic |
| AATATGTTAATGCTTGAGAG+CGG | 0.775120 | 8.3:+8485498 | MS.gene036585:CDS |
| ATATGATACATATGCCAACG+AGG | 0.781154 | 8.3:+8490974 | MS.gene036585:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 8483822 | 8491229 | 8483822 | ID=MS.gene036585 |
| chr8.3 | mRNA | 8483822 | 8491229 | 8483822 | ID=MS.gene036585.t1;Parent=MS.gene036585 |
| chr8.3 | exon | 8483822 | 8483935 | 8483822 | ID=MS.gene036585.t1.exon1;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8483822 | 8483935 | 8483822 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8484174 | 8484257 | 8484174 | ID=MS.gene036585.t1.exon2;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8484174 | 8484257 | 8484174 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8484373 | 8484444 | 8484373 | ID=MS.gene036585.t1.exon3;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8484373 | 8484444 | 8484373 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8484539 | 8484634 | 8484539 | ID=MS.gene036585.t1.exon4;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8484539 | 8484634 | 8484539 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8484734 | 8484970 | 8484734 | ID=MS.gene036585.t1.exon5;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8484734 | 8484970 | 8484734 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8485477 | 8485587 | 8485477 | ID=MS.gene036585.t1.exon6;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8485477 | 8485587 | 8485477 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8485676 | 8485789 | 8485676 | ID=MS.gene036585.t1.exon7;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8485676 | 8485789 | 8485676 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8488982 | 8489107 | 8488982 | ID=MS.gene036585.t1.exon8;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8488982 | 8489107 | 8488982 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8489221 | 8489289 | 8489221 | ID=MS.gene036585.t1.exon9;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8489221 | 8489289 | 8489221 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8489390 | 8489496 | 8489390 | ID=MS.gene036585.t1.exon10;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8489390 | 8489496 | 8489390 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8489761 | 8489844 | 8489761 | ID=MS.gene036585.t1.exon11;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8489761 | 8489844 | 8489761 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8489955 | 8490042 | 8489955 | ID=MS.gene036585.t1.exon12;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8489955 | 8490042 | 8489955 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8490298 | 8490417 | 8490298 | ID=MS.gene036585.t1.exon13;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8490298 | 8490417 | 8490298 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8490603 | 8490787 | 8490603 | ID=MS.gene036585.t1.exon14;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8490603 | 8490787 | 8490603 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| chr8.3 | exon | 8490929 | 8491229 | 8490929 | ID=MS.gene036585.t1.exon15;Parent=MS.gene036585.t1 |
| chr8.3 | CDS | 8490929 | 8491229 | 8490929 | ID=cds.MS.gene036585.t1;Parent=MS.gene036585.t1 |
| Gene Sequence |
| Protein sequence |
