Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041467.t1 | XP_013458203.1 | 97 | 635 | 18 | 1 | 1 | 634 | 1 | 635 | 0.00E+00 | 1209.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041467.t1 | Q9NYV6 | 27.7 | 573 | 361 | 17 | 32 | 572 | 58 | 609 | 7.0e-36 | 153.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041467.t1 | A0A072UQX1 | 97.0 | 635 | 18 | 1 | 1 | 634 | 1 | 635 | 0.0e+00 | 1209.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene033712 | MS.gene041467 | PPI |
| MS.gene033714 | MS.gene041467 | PPI |
| MS.gene030166 | MS.gene041467 | PPI |
| MS.gene26516 | MS.gene041467 | PPI |
| MS.gene065392 | MS.gene041467 | PPI |
| MS.gene035233 | MS.gene041467 | PPI |
| MS.gene024279 | MS.gene041467 | PPI |
| MS.gene036585 | MS.gene041467 | PPI |
| MS.gene26777 | MS.gene041467 | PPI |
| MS.gene73391 | MS.gene041467 | PPI |
| MS.gene68633 | MS.gene041467 | PPI |
| MS.gene047813 | MS.gene041467 | PPI |
| MS.gene053492 | MS.gene041467 | PPI |
| MS.gene39636 | MS.gene041467 | PPI |
| MS.gene064140 | MS.gene041467 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041467.t1 | MTR_4g117680 | 97.008 | 635 | 18 | 1 | 1 | 634 | 1 | 635 | 0.0 | 1265 |
| MS.gene041467.t1 | MTR_8g085920 | 86.076 | 79 | 11 | 0 | 193 | 271 | 1 | 79 | 4.75e-41 | 145 |
| MS.gene041467.t1 | MTR_4g117730 | 68.657 | 67 | 21 | 0 | 205 | 271 | 1 | 67 | 8.66e-19 | 81.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041467.t1 | AT2G34750 | 46.093 | 627 | 294 | 12 | 17 | 633 | 21 | 613 | 2.92e-175 | 512 |
| MS.gene041467.t1 | AT1G30590 | 45.513 | 624 | 300 | 13 | 17 | 634 | 17 | 606 | 8.98e-169 | 495 |
| MS.gene041467.t1 | AT1G30590 | 45.513 | 624 | 300 | 13 | 17 | 634 | 17 | 606 | 8.98e-169 | 495 |
| MS.gene041467.t1 | AT1G30590 | 45.513 | 624 | 297 | 14 | 17 | 634 | 17 | 603 | 1.19e-166 | 489 |
| MS.gene041467.t1 | AT2G39240 | 40.126 | 633 | 288 | 15 | 17 | 629 | 7 | 568 | 5.94e-126 | 384 |
| MS.gene041467.t1 | AT2G39240 | 40.126 | 633 | 288 | 15 | 17 | 629 | 7 | 568 | 5.94e-126 | 384 |
Find 116 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTATGAAATATGGGTTTC+AGG | 0.216795 | 8.2:+11798090 | MS.gene041467:CDS |
| ACGCCCAAATCTATGAAATA+TGG | 0.220265 | 8.2:+11798082 | MS.gene041467:CDS |
| AGAAATTCAAAGGCGTATTT+AGG | 0.222570 | 8.2:-11791779 | None:intergenic |
| AAACCCATATTTCATAGATT+TGG | 0.238800 | 8.2:-11798086 | None:intergenic |
| TCCTGGTTGTAGGTGGAAAT+TGG | 0.276044 | 8.2:+11792627 | MS.gene041467:intron |
| AACCCATATTTCATAGATTT+GGG | 0.308026 | 8.2:-11798085 | None:intergenic |
| GGCTCCTTAGCGAAACGTTT+TGG | 0.310138 | 8.2:-11791929 | None:intergenic |
| TTCAACAAACATGGATCAAA+TGG | 0.328326 | 8.2:-11797701 | None:intergenic |
| AAATGCCCATGGAGCTAATC+TGG | 0.332385 | 8.2:+11797319 | MS.gene041467:CDS |
| ATGCTTGAGAGCGGTGAAAT+TGG | 0.343084 | 8.2:+11792470 | MS.gene041467:CDS |
| GTACCACAGAGTTGTTAAAT+AGG | 0.350184 | 8.2:+11795933 | MS.gene041467:CDS |
| TCACGACGCTGCTAAACTTT+TGG | 0.352317 | 8.2:+11791217 | MS.gene041467:CDS |
| GCTTGAGCTCATTGTGTCAT+TGG | 0.362045 | 8.2:+11791594 | MS.gene041467:CDS |
| GGCATACTGTGGACAACTAT+AGG | 0.373269 | 8.2:-11791908 | None:intergenic |
| GCGGATCTTTCCAAGGCTTT+CGG | 0.378275 | 8.2:+11797650 | MS.gene041467:CDS |
| TGGAGTTGACATGAAGAATA+AGG | 0.387882 | 8.2:+11791814 | MS.gene041467:CDS |
| GGATATGAATGATTATGATA+TGG | 0.391946 | 8.2:+11798003 | MS.gene041467:CDS |
| TCTGAAACACATAGCTGATT+TGG | 0.393971 | 8.2:+11791859 | MS.gene041467:CDS |
| TAAATTGTTCGTTACCTTCA+AGG | 0.395606 | 8.2:-11797358 | None:intergenic |
| AAGCGGCATTACCTAGTAAT+AGG | 0.412363 | 8.2:-11796433 | None:intergenic |
| AGTAGAGGAATTTCTAAAAC+AGG | 0.414435 | 8.2:+11797568 | MS.gene041467:CDS |
| CGCCCAAATCTATGAAATAT+GGG | 0.416002 | 8.2:+11798083 | MS.gene041467:CDS |
| AAAATTTACACCACATCCTT+CGG | 0.424276 | 8.2:-11796368 | None:intergenic |
| TGATGAGAAATATTATAGTA+TGG | 0.433744 | 8.2:+11792728 | MS.gene041467:CDS |
| GAACAATTTGCAAGGCAATC+AGG | 0.437527 | 8.2:+11795955 | MS.gene041467:CDS |
| CTTTCGCATGAGATCATTGA+TGG | 0.440574 | 8.2:+11797266 | MS.gene041467:CDS |
| ATAAGCTTCACTTTAAGTCT+AGG | 0.441192 | 8.2:-11797294 | None:intergenic |
| TGACCATCTCCAGCTACTTA+AGG | 0.454991 | 8.2:+11797864 | MS.gene041467:intron |
| AGCTTTCGAGATTGAGCTTG+TGG | 0.456347 | 8.2:+11791518 | MS.gene041467:intron |
| GGGATTATGCTCCAACTGAT+AGG | 0.461972 | 8.2:+11791539 | MS.gene041467:CDS |
| GAAGTTTCTTTCATCTGAGT+TGG | 0.464213 | 8.2:+11796750 | MS.gene041467:CDS |
| GTGTTAATCCTCCTATTACT+AGG | 0.469232 | 8.2:+11796422 | MS.gene041467:CDS |
| AACGAGGAGCTCAGTGATAG+TGG | 0.472727 | 8.2:+11797938 | MS.gene041467:CDS |
| TGGATGGGATGGCAAGATAC+AGG | 0.478954 | 8.2:+11792647 | MS.gene041467:CDS |
| ATTTATCCCACCGAAAGCCT+TGG | 0.486236 | 8.2:-11797660 | None:intergenic |
| AGAAATTCCTCTACTACGCT+AGG | 0.486325 | 8.2:-11797560 | None:intergenic |
| GCTTTCGAGATTGAGCTTGT+GGG | 0.486729 | 8.2:+11791519 | MS.gene041467:intron |
| CAACGAAGATTTATCTGTCA+TGG | 0.487257 | 8.2:+11790820 | MS.gene041467:CDS |
| ATGTTTCCAGATTAGCTCCA+TGG | 0.488983 | 8.2:-11797325 | None:intergenic |
| AGATACCCTTCTTGCAGTCT+CGG | 0.490560 | 8.2:+11790889 | MS.gene041467:CDS |
| GTGATAATCGCAAGGGAGCA+AGG | 0.492980 | 8.2:-11791886 | None:intergenic |
| GATCTTTCCAAGGCTTTCGG+TGG | 0.493557 | 8.2:+11797653 | MS.gene041467:CDS |
| CTCCATTGCAAATATGCACT+TGG | 0.495884 | 8.2:-11792676 | None:intergenic |
| GATAAATCTTCGTTGACAAT+CGG | 0.496395 | 8.2:-11790812 | None:intergenic |
| AAATTGTTCGTTACCTTCAA+GGG | 0.496820 | 8.2:-11797357 | None:intergenic |
| GGTGAAATTGGAGAAATTGT+TGG | 0.498711 | 8.2:+11792482 | MS.gene041467:CDS |
| GAAGCGATTCATGCTGGATA+GGG | 0.499853 | 8.2:-11791395 | None:intergenic |
| ATGAATGATTATGATATGGC+CGG | 0.501292 | 8.2:+11798007 | MS.gene041467:CDS |
| AGAAGCGATTCATGCTGGAT+AGG | 0.502609 | 8.2:-11791396 | None:intergenic |
| GAAGAATAAGGTTCTGCCTC+GGG | 0.505488 | 8.2:+11791826 | MS.gene041467:CDS |
| ACATAAATTGAATCCCTTGA+AGG | 0.505777 | 8.2:+11797344 | MS.gene041467:CDS |
| GCATCATATTTCCTATCAGT+TGG | 0.506860 | 8.2:-11791550 | None:intergenic |
| ATTCTCGTGGTTCAGGATGG+TGG | 0.508749 | 8.2:+11791140 | MS.gene041467:intron |
| TTTCATAGATTTGGGCGTGA+TGG | 0.514294 | 8.2:-11798077 | None:intergenic |
| TGTTTCCAGATTAGCTCCAT+GGG | 0.520405 | 8.2:-11797324 | None:intergenic |
| AAGAAAGAAGCGATTCATGC+TGG | 0.521142 | 8.2:-11791401 | None:intergenic |
| TACAATACTACCTGCACTTG+TGG | 0.523666 | 8.2:+11792508 | MS.gene041467:CDS |
| ATCAACTTATCCACAAGTGC+AGG | 0.525390 | 8.2:-11792518 | None:intergenic |
| GCTCGAGGCGGATCTTTCCA+AGG | 0.526827 | 8.2:+11797643 | MS.gene041467:CDS |
| ATATGATGCAGCTGATATCA+TGG | 0.528281 | 8.2:+11791564 | MS.gene041467:CDS |
| GGTTGTAGGTGGAAATTGGA+TGG | 0.531254 | 8.2:+11792631 | MS.gene041467:intron |
| TAGGTGGAAATTGGATGGGA+TGG | 0.531448 | 8.2:+11792636 | MS.gene041467:intron |
| TCAACAAACATGGATCAAAT+GGG | 0.531713 | 8.2:-11797700 | None:intergenic |
| ATATGGGTTTCAGGCACAGA+TGG | 0.533433 | 8.2:+11798099 | MS.gene041467:CDS |
| ATCTTTCCAAGGCTTTCGGT+GGG | 0.536074 | 8.2:+11797654 | MS.gene041467:CDS |
| AATTCAAAGGCGTATTTAGG+AGG | 0.538124 | 8.2:-11791776 | None:intergenic |
| TGTGGATAAGTTGATAGAGT+TGG | 0.541891 | 8.2:+11792526 | MS.gene041467:CDS |
| TGATAATCGCAAGGGAGCAA+GGG | 0.543178 | 8.2:-11791885 | None:intergenic |
| GGAATTTCTAAAACAGGCAA+AGG | 0.549923 | 8.2:+11797574 | MS.gene041467:CDS |
| TAAAACAGGCAAAGGCTGCT+CGG | 0.551011 | 8.2:+11797582 | MS.gene041467:CDS |
| CTTGACCAGCGGACAAAATG+TGG | 0.555293 | 8.2:-11797887 | None:intergenic |
| TCTTGAATGCTGTCAAAGCA+AGG | 0.555849 | 8.2:+11796021 | MS.gene041467:CDS |
| AACTATAGGTGATAATCGCA+AGG | 0.557758 | 8.2:-11791894 | None:intergenic |
| GGTTTCAGGCACAGATGGAA+GGG | 0.558054 | 8.2:+11798104 | MS.gene041467:CDS |
| GGGTTTCAGGCACAGATGGA+AGG | 0.558736 | 8.2:+11798103 | MS.gene041467:CDS |
| TGAAGAATAAGGTTCTGCCT+CGG | 0.559544 | 8.2:+11791825 | MS.gene041467:CDS |
| CTGTCAAAGCAAGGGCCGAT+TGG | 0.561772 | 8.2:+11796030 | MS.gene041467:CDS |
| AAGCAAGGGCCGATTGGCTG+AGG | 0.562906 | 8.2:+11796036 | MS.gene041467:CDS |
| TTGGGAATACCTCAGCCAAT+CGG | 0.566052 | 8.2:-11796045 | None:intergenic |
| TTAAATAGGAACAATTTGCA+AGG | 0.569913 | 8.2:+11795947 | MS.gene041467:CDS |
| TGCCAAGTGCATATTTGCAA+TGG | 0.570882 | 8.2:+11792674 | MS.gene041467:CDS |
| AGATTTATCTGTCATGGAGG+AGG | 0.573181 | 8.2:+11790826 | MS.gene041467:CDS |
| ATACAAGTCAAAATTTGCCC+AGG | 0.575195 | 8.2:+11796218 | MS.gene041467:CDS |
| AAGACCCGCTTCAACACTCC+CGG | 0.576390 | 8.2:-11798026 | None:intergenic |
| GATGTTCTATGCATGCGCAT+TGG | 0.580066 | 8.2:+11796342 | MS.gene041467:CDS |
| TGAATGATTATGATATGGCC+GGG | 0.586844 | 8.2:+11798008 | MS.gene041467:CDS |
| ATGCTGGATAGGGTCAATGT+AGG | 0.592083 | 8.2:-11791385 | None:intergenic |
| TTGCAAACACTTAACAAGCT+CGG | 0.593747 | 8.2:-11791174 | None:intergenic |
| ATGCTTGCCTAGCGTAGTAG+AGG | 0.596859 | 8.2:+11797553 | MS.gene041467:CDS |
| CGGCATTACCTAGTAATAGG+AGG | 0.597341 | 8.2:-11796430 | None:intergenic |
| ACTATCACTGAGCTCCTCGT+TGG | 0.597389 | 8.2:-11797936 | None:intergenic |
| GCTTACCGAGACTGCAAGAA+GGG | 0.601568 | 8.2:-11790894 | None:intergenic |
| GTTGTAGGTGGAAATTGGAT+GGG | 0.602234 | 8.2:+11792632 | MS.gene041467:intron |
| ATGGCCGGGAGTGTTGAAGC+GGG | 0.607938 | 8.2:+11798022 | MS.gene041467:CDS |
| GAAGCTTATCAAAATGCCCA+TGG | 0.616331 | 8.2:+11797308 | MS.gene041467:CDS |
| GGCTTACCGAGACTGCAAGA+AGG | 0.617000 | 8.2:-11790895 | None:intergenic |
| TATGCCAAAACGTTTCGCTA+AGG | 0.621248 | 8.2:+11791925 | MS.gene041467:CDS |
| TTCAGAGCAGCATGCACCCG+AGG | 0.627619 | 8.2:-11791842 | None:intergenic |
| GAAATTGGAGAAATTGTTGG+TGG | 0.630468 | 8.2:+11792485 | MS.gene041467:CDS |
| GATAATCGCAAGGGAGCAAG+GGG | 0.631795 | 8.2:-11791884 | None:intergenic |
| CTTGAATGCTGTCAAAGCAA+GGG | 0.633580 | 8.2:+11796022 | MS.gene041467:CDS |
| TCGCATGAGATCATTGATGG+AGG | 0.634684 | 8.2:+11797269 | MS.gene041467:CDS |
| GTTCCTATTTAACAACTCTG+TGG | 0.638060 | 8.2:-11795936 | None:intergenic |
| GTTTGTTGAAGAAAAGTGAA+AGG | 0.640363 | 8.2:+11797714 | MS.gene041467:CDS |
| TATGGCCGGGAGTGTTGAAG+CGG | 0.646112 | 8.2:+11798021 | MS.gene041467:CDS |
| GCATGCGCATTGGATTCCGA+AGG | 0.647857 | 8.2:+11796352 | MS.gene041467:CDS |
| TGGTTGCTAACATCATACAA+AGG | 0.652099 | 8.2:+11796770 | MS.gene041467:CDS |
| GCATTGGATTCCGAAGGATG+TGG | 0.661172 | 8.2:+11796358 | MS.gene041467:CDS |
| CGAAGATTTATCTGTCATGG+AGG | 0.674048 | 8.2:+11790823 | MS.gene041467:CDS |
| TTTCGATGATTTGCTCGAGG+CGG | 0.688171 | 8.2:+11797631 | MS.gene041467:CDS |
| AAACATCTCAGTGAGGACCA+TGG | 0.694262 | 8.2:-11796392 | None:intergenic |
| ACTATAGGTGATAATCGCAA+GGG | 0.699606 | 8.2:-11791893 | None:intergenic |
| ATGGGATGGCAAGATACAGG+AGG | 0.711153 | 8.2:+11792650 | MS.gene041467:CDS |
| ATGAATCAAACATCTCAGTG+AGG | 0.761804 | 8.2:-11796399 | None:intergenic |
| AATATGTTAATGCTTGAGAG+CGG | 0.775120 | 8.2:+11792461 | MS.gene041467:CDS |
| ATATGATACATATGCCAACG+AGG | 0.781154 | 8.2:+11797922 | MS.gene041467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 11790797 | 11798177 | 11790797 | ID=MS.gene041467 |
| chr8.2 | mRNA | 11790797 | 11798177 | 11790797 | ID=MS.gene041467.t1;Parent=MS.gene041467 |
| chr8.2 | exon | 11790797 | 11790910 | 11790797 | ID=MS.gene041467.t1.exon1;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11790797 | 11790910 | 11790797 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11791155 | 11791238 | 11791155 | ID=MS.gene041467.t1.exon2;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11791155 | 11791238 | 11791155 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11791354 | 11791425 | 11791354 | ID=MS.gene041467.t1.exon3;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11791354 | 11791425 | 11791354 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11791520 | 11791615 | 11791520 | ID=MS.gene041467.t1.exon4;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11791520 | 11791615 | 11791520 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11791716 | 11791952 | 11791716 | ID=MS.gene041467.t1.exon5;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11791716 | 11791952 | 11791716 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11792440 | 11792550 | 11792440 | ID=MS.gene041467.t1.exon6;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11792440 | 11792550 | 11792440 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11792639 | 11792749 | 11792639 | ID=MS.gene041467.t1.exon7;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11792639 | 11792749 | 11792639 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11795932 | 11796057 | 11795932 | ID=MS.gene041467.t1.exon8;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11795932 | 11796057 | 11795932 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11796171 | 11796239 | 11796171 | ID=MS.gene041467.t1.exon9;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11796171 | 11796239 | 11796171 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11796337 | 11796443 | 11796337 | ID=MS.gene041467.t1.exon10;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11796337 | 11796443 | 11796337 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11796708 | 11796791 | 11796708 | ID=MS.gene041467.t1.exon11;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11796708 | 11796791 | 11796708 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11796902 | 11796989 | 11796902 | ID=MS.gene041467.t1.exon12;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11796902 | 11796989 | 11796902 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11797246 | 11797365 | 11797246 | ID=MS.gene041467.t1.exon13;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11797246 | 11797365 | 11797246 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11797551 | 11797735 | 11797551 | ID=MS.gene041467.t1.exon14;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11797551 | 11797735 | 11797551 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| chr8.2 | exon | 11797877 | 11798177 | 11797877 | ID=MS.gene041467.t1.exon15;Parent=MS.gene041467.t1 |
| chr8.2 | CDS | 11797877 | 11798177 | 11797877 | ID=cds.MS.gene041467.t1;Parent=MS.gene041467.t1 |
| Gene Sequence |
| Protein sequence |
