Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03589.t1 | XP_013443148.1 | 97.8 | 274 | 6 | 0 | 1 | 274 | 1 | 274 | 1.60E-142 | 515.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03589.t1 | Q9FJJ5 | 74.6 | 264 | 64 | 2 | 1 | 263 | 1 | 262 | 7.5e-104 | 378.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03589.t1 | A0A072TJV8 | 97.8 | 274 | 6 | 0 | 1 | 274 | 1 | 274 | 1.2e-142 | 515.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene58445 | MS.gene03589 | PPI |
| MS.gene20245 | MS.gene03589 | PPI |
| MS.gene03589 | MS.gene018958 | PPI |
| MS.gene015206 | MS.gene03589 | PPI |
| MS.gene03589 | MS.gene06707 | PPI |
| MS.gene06926 | MS.gene03589 | PPI |
| MS.gene03589 | MS.gene09540 | PPI |
| MS.gene03589 | MS.gene015202 | PPI |
| MS.gene03589 | MS.gene033134 | PPI |
| MS.gene03589 | MS.gene50990 | PPI |
| MS.gene03589 | MS.gene09543 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03589.t1 | MTR_0036s0210 | 97.810 | 274 | 6 | 0 | 1 | 274 | 1 | 274 | 0.0 | 555 |
| MS.gene03589.t1 | MTR_4g108330 | 76.923 | 273 | 58 | 3 | 1 | 270 | 1 | 271 | 6.13e-155 | 433 |
| MS.gene03589.t1 | MTR_5g016520 | 58.304 | 283 | 95 | 5 | 1 | 267 | 1 | 276 | 3.85e-109 | 319 |
| MS.gene03589.t1 | MTR_2072s0010 | 92.308 | 52 | 4 | 0 | 37 | 88 | 1 | 52 | 2.58e-26 | 98.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene03589.t1 | AT5G62500 | 74.621 | 264 | 64 | 2 | 1 | 263 | 1 | 262 | 1.98e-145 | 409 |
| MS.gene03589.t1 | AT3G47690 | 72.000 | 275 | 61 | 4 | 1 | 271 | 1 | 263 | 6.62e-142 | 400 |
| MS.gene03589.t1 | AT5G67270 | 55.926 | 270 | 103 | 4 | 1 | 256 | 1 | 268 | 2.92e-103 | 304 |
Find 57 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGTCATGCTTCAGTTTC+TGG | 0.116159 | 3.4:+31982099 | None:intergenic |
| ACCGAAATGTTAAGAGTTTA+AGG | 0.172808 | 3.4:-31983688 | MS.gene03589:CDS |
| AGCTAACCTGAGTTTCTATT+TGG | 0.188642 | 3.4:+31983608 | None:intergenic |
| TCCCGTGGAGCGTAGGATTA+AGG | 0.235106 | 3.4:-31983720 | MS.gene03589:CDS |
| CGTTACTGTGATTCTGTTAA+TGG | 0.239870 | 3.4:-31984000 | MS.gene03589:CDS |
| TCCTTAAACTCTTAACATTT+CGG | 0.298932 | 3.4:+31983687 | None:intergenic |
| CCAGTTGACCTTGATATCTT+AGG | 0.307940 | 3.4:+31983511 | None:intergenic |
| GAAGTTAACAGGCTTGTTAA+AGG | 0.316943 | 3.4:-31984063 | MS.gene03589:CDS |
| CCCGTGGAGCGTAGGATTAA+GGG | 0.337587 | 3.4:-31983719 | MS.gene03589:CDS |
| GGCAGCATATTGAAGTTAAC+AGG | 0.350762 | 3.4:-31984074 | MS.gene03589:intron |
| CATTCTCCAGTTCTGATGTC+TGG | 0.371007 | 3.4:+31982876 | None:intergenic |
| TAATTCGTCAGCAGAGATTC+AGG | 0.371636 | 3.4:-31983473 | MS.gene03589:CDS |
| ATTCATTGCTAGGCTGCATC+TGG | 0.385662 | 3.4:-31988122 | MS.gene03589:intron |
| CAAAATCTCAGTGGATCTCT+TGG | 0.386039 | 3.4:-31982951 | MS.gene03589:CDS |
| TGCAAAAGAGTCGGCATTAG+AGG | 0.399645 | 3.4:-31982169 | MS.gene03589:CDS |
| GGCGACGAGTATAGGGATAA+TGG | 0.410039 | 3.4:-31989232 | MS.gene03589:CDS |
| TCAAACACTACGAATAATAC+TGG | 0.415660 | 3.4:-31983647 | MS.gene03589:CDS |
| GTCGGCATTAGAGGAAGCTC+AGG | 0.421605 | 3.4:-31982160 | MS.gene03589:CDS |
| GCTTGTTAAAGGCCGACCTT+TGG | 0.439334 | 3.4:-31984052 | MS.gene03589:CDS |
| ATGTTAGACATGACGTATCC+AGG | 0.448200 | 3.4:-31988083 | MS.gene03589:CDS |
| CCTAAGATATCAAGGTCAAC+TGG | 0.449224 | 3.4:-31983511 | MS.gene03589:CDS |
| TTGTGCATCGGCACAACCCC+TGG | 0.449890 | 3.4:+31988065 | None:intergenic |
| AGGCCGACCTTTGGACAACT+TGG | 0.450161 | 3.4:-31984043 | MS.gene03589:CDS |
| TCAAAACTATAAAGTTCTAC+AGG | 0.481633 | 3.4:-31987913 | MS.gene03589:CDS |
| TCTATGCCAGACATCAGAAC+TGG | 0.488709 | 3.4:-31982882 | MS.gene03589:CDS |
| AGGTCAACTGGAGGATCGGA+TGG | 0.491495 | 3.4:-31983499 | MS.gene03589:CDS |
| ACTACGAATAATACTGGTTC+AGG | 0.494201 | 3.4:-31983641 | MS.gene03589:CDS |
| AGTAACGTTTCAGCCATTGT+AGG | 0.497462 | 3.4:+31984016 | None:intergenic |
| CCCTTAATCCTACGCTCCAC+GGG | 0.509614 | 3.4:+31983719 | None:intergenic |
| GATACGTCATGTCTAACATC+TGG | 0.514396 | 3.4:+31988087 | None:intergenic |
| ATCAAGGTCAACTGGAGGAT+CGG | 0.530947 | 3.4:-31983503 | MS.gene03589:CDS |
| GGTCAACTGGAGGATCGGAT+GGG | 0.532130 | 3.4:-31983498 | MS.gene03589:CDS |
| GATTCAGGCCTTGTCCAAGC+AGG | 0.534074 | 3.4:-31983458 | MS.gene03589:intron |
| ACTATAATCCCGTGGAGCGT+AGG | 0.534515 | 3.4:-31983727 | MS.gene03589:CDS |
| ACCCTTAATCCTACGCTCCA+CGG | 0.538190 | 3.4:+31983718 | None:intergenic |
| TTACATTAATCAAACATTGG+AGG | 0.542731 | 3.4:-31982136 | MS.gene03589:CDS |
| CTAAGTCCAAATAGAAACTC+AGG | 0.542973 | 3.4:-31983614 | MS.gene03589:intron |
| GGCTTACATTAATCAAACAT+TGG | 0.556676 | 3.4:-31982139 | MS.gene03589:CDS |
| GTGTAGGACCTAAGATATCA+AGG | 0.558318 | 3.4:-31983519 | MS.gene03589:intron |
| GTCAACTGGAGGATCGGATG+GGG | 0.564150 | 3.4:-31983497 | MS.gene03589:CDS |
| TGTTAGACATGACGTATCCA+GGG | 0.569863 | 3.4:-31988082 | MS.gene03589:CDS |
| AAGTTGTACCTGCTTGGACA+AGG | 0.572645 | 3.4:+31983450 | None:intergenic |
| TAATCTCTCTCGCATTGAAG+AGG | 0.585059 | 3.4:-31989148 | MS.gene03589:intron |
| CAACAAGCTGAAAATTGCAA+AGG | 0.596592 | 3.4:-31987883 | MS.gene03589:intron |
| GCGTAGGATTAAGGGTGGAA+AGG | 0.606069 | 3.4:-31983711 | MS.gene03589:CDS |
| TGTGTTGCTACCTTGTGCAT+CGG | 0.608007 | 3.4:+31988053 | None:intergenic |
| GTAGAAATGAGATTCTGAGT+TGG | 0.610210 | 3.4:-31989191 | MS.gene03589:CDS |
| AGGGGTTGTGCCGATGCACA+AGG | 0.630070 | 3.4:-31988063 | MS.gene03589:intron |
| ACTCCAAGTTGTCCAAAGGT+CGG | 0.639935 | 3.4:+31984040 | None:intergenic |
| TGCTGCTGATGCAAAAGAGT+CGG | 0.643139 | 3.4:-31982178 | MS.gene03589:CDS |
| AGGAACTCCAAGTTGTCCAA+AGG | 0.653402 | 3.4:+31984036 | None:intergenic |
| TACTGATCTCAAAATCTCAG+TGG | 0.654360 | 3.4:-31982960 | MS.gene03589:CDS |
| TACTGTGATTCTGTTAATGG+TGG | 0.661559 | 3.4:-31983997 | MS.gene03589:CDS |
| ACAACTTGGAGTTCCTACAA+TGG | 0.679808 | 3.4:-31984029 | MS.gene03589:CDS |
| GTGGAGCGTAGGATTAAGGG+TGG | 0.682025 | 3.4:-31983716 | MS.gene03589:CDS |
| AAGATATCAAGGTCAACTGG+AGG | 0.692502 | 3.4:-31983508 | MS.gene03589:CDS |
| GTTAGACATGACGTATCCAG+GGG | 0.713529 | 3.4:-31988081 | MS.gene03589:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 31982104 | 31989256 | 31982104 | ID=MS.gene03589 |
| chr3.4 | mRNA | 31982104 | 31989256 | 31982104 | ID=MS.gene03589.t1;Parent=MS.gene03589 |
| chr3.4 | exon | 31989149 | 31989256 | 31989149 | ID=MS.gene03589.t1.exon1;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31989149 | 31989256 | 31989149 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31988064 | 31988132 | 31988064 | ID=MS.gene03589.t1.exon2;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31988064 | 31988132 | 31988064 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31987884 | 31987970 | 31987884 | ID=MS.gene03589.t1.exon3;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31987884 | 31987970 | 31987884 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31983985 | 31984091 | 31983985 | ID=MS.gene03589.t1.exon4;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31983985 | 31984091 | 31983985 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31983615 | 31983751 | 31983615 | ID=MS.gene03589.t1.exon5;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31983615 | 31983751 | 31983615 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31983459 | 31983535 | 31983459 | ID=MS.gene03589.t1.exon6;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31983459 | 31983535 | 31983459 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31982871 | 31982984 | 31982871 | ID=MS.gene03589.t1.exon7;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31982871 | 31982984 | 31982871 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| chr3.4 | exon | 31982104 | 31982229 | 31982104 | ID=MS.gene03589.t1.exon8;Parent=MS.gene03589.t1 |
| chr3.4 | CDS | 31982104 | 31982229 | 31982104 | ID=cds.MS.gene03589.t1;Parent=MS.gene03589.t1 |
| Gene Sequence |
| Protein sequence |
