AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043576

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043576.t1 MTR_4g121550 86.154 325 40 2 1 321 1 324 0.0 576
MS.gene043576.t1 MTR_2g093790 77.329 322 26 5 1 322 1 275 1.03e-171 477
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043576.t1 AT1G03360 61.774 327 110 6 1 321 3 320 3.53e-145 412

Find 52 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGAGTATTGTCCACTTT+AGG 0.290848 2.2:+7263150 MS.gene043576:CDS
AAGGCTGGTGACATTGTTAT+AGG 0.303613 2.2:+7259673 MS.gene043576:CDS
TGGTTCCGAGTTCTGTGTTC+TGG 0.316171 2.2:+7263251 MS.gene043576:CDS
CTTTGAGTACGCGATCTTCA+TGG 0.342305 2.2:-7259540 None:intergenic
ATCTTCTATCATGTCATCTC+TGG 0.353592 2.2:-7263004 None:intergenic
ACGCGATCTTCATGGTTGAT+AGG 0.365746 2.2:-7259532 None:intergenic
AGACAAGTGAATACATTTGC+AGG 0.378721 2.2:+7263115 MS.gene043576:CDS
TTAAGACTTGTGGATTAGAT+TGG 0.389862 2.2:-7263035 None:intergenic
TATGGTATTGATTTAATACT+TGG 0.403711 2.2:+7262943 MS.gene043576:CDS
GATTTAATACTTGGCTGTAA+TGG 0.406532 2.2:+7262952 MS.gene043576:CDS
TTGGCTGTAATGGATTCATA+TGG 0.413004 2.2:+7262962 MS.gene043576:CDS
TATAACAATGTCACCAGCCT+TGG 0.414901 2.2:-7259671 None:intergenic
AAACCAACATTTCCATCATC+TGG 0.418415 2.2:+7262915 MS.gene043576:CDS
TGGTCAGAATCGTTGGAGAT+TGG 0.435250 2.2:+7259802 MS.gene043576:CDS
AGGCTGGTGACATTGTTATA+GGG 0.439117 2.2:+7259674 MS.gene043576:CDS
GTTGTATTGCAGCTTAGTAC+TGG 0.459996 2.2:+7262853 MS.gene043576:intron
TTCCATCATCTGGAGCAGTA+TGG 0.460289 2.2:+7262925 MS.gene043576:CDS
ATAACAATGTCACCAGCCTT+GGG 0.467609 2.2:-7259670 None:intergenic
CGCGATCTTCATGGTTGATA+GGG 0.468771 2.2:-7259531 None:intergenic
TGCTACTCTCTGTGGTGTTG+TGG 0.476983 2.2:+7259597 MS.gene043576:CDS
GAATATGCCCGATGGTCTAC+AGG 0.480017 2.2:+7259868 MS.gene043576:CDS
ATCTTCTGATTCTTCTGTGA+CGG 0.494275 2.2:+7259495 MS.gene043576:CDS
TTTAACACTTGAAATCATCA+AGG 0.502649 2.2:+7263179 MS.gene043576:CDS
CAAAATACCTGTAGACCATC+GGG 0.507180 2.2:-7259875 None:intergenic
TACCATACTGCTCCAGATGA+TGG 0.509092 2.2:-7262927 None:intergenic
CTGAAAATCACGAACTACTT+CGG 0.517598 2.2:-7262504 None:intergenic
TGTTAAAATGAATCCTAAAG+TGG 0.518211 2.2:-7263163 None:intergenic
TGTAGGTTGGTCAGAATCGT+TGG 0.520122 2.2:+7259795 MS.gene043576:intron
TGCAACCAGAACACAGAACT+CGG 0.532336 2.2:-7263256 None:intergenic
TCAAGATACAAACCCAAGGC+TGG 0.533344 2.2:+7259658 MS.gene043576:CDS
ATCTTCATGGTTGATAGGGA+TGG 0.535963 2.2:-7259527 None:intergenic
CTTGATATACACGACATGCT+TGG 0.537424 2.2:+7263231 MS.gene043576:CDS
CTTTCCATTGCTAGAGGCGA+AGG 0.541185 2.2:+7262334 MS.gene043576:intron
CGAGTGTAACAGAGATGAGA+AGG 0.544062 2.2:+7259404 None:intergenic
GCAAAATACCTGTAGACCAT+CGG 0.552621 2.2:-7259876 None:intergenic
TTAACACTTGAAATCATCAA+GGG 0.558961 2.2:+7263180 MS.gene043576:CDS
GTTCCAGTGCCTTCTGTAGA+CGG 0.559019 2.2:-7259459 None:intergenic
TTATTGGGAATTAAGACTTG+TGG 0.572669 2.2:-7263045 None:intergenic
TGGCTGTAATGGATTCATAT+GGG 0.574523 2.2:+7262963 MS.gene043576:CDS
GCTCCAGATGATGGAAATGT+TGG 0.581478 2.2:-7262918 None:intergenic
CATGAAGATCGCGTACTCAA+AGG 0.588607 2.2:+7259541 MS.gene043576:CDS
GATCCGTCTACAGAAGGCAC+TGG 0.590591 2.2:+7259456 MS.gene043576:CDS
TGTAATGGATTCATATGGGT+CGG 0.597510 2.2:+7262967 MS.gene043576:CDS
TCAGAAGATCCGTCTACAGA+AGG 0.616298 2.2:+7259450 MS.gene043576:CDS
GACAAGTGAATACATTTGCA+GGG 0.619292 2.2:+7263116 MS.gene043576:CDS
CATGGAACCGCTGATATCAA+CGG 0.640741 2.2:+7259565 MS.gene043576:CDS
TCTGCTATGAATATGCCCGA+TGG 0.653631 2.2:+7259860 MS.gene043576:CDS
AACTTCGCCGTTGATATCAG+CGG 0.663408 2.2:-7259572 None:intergenic
GAAGTTGTTGCTACTCTCTG+TGG 0.664301 2.2:+7259589 MS.gene043576:CDS
GATCGCGTACTCAAAGGACA+TGG 0.665690 2.2:+7259547 MS.gene043576:CDS
GCGCTCAAGATACAAACCCA+AGG 0.676543 2.2:+7259654 MS.gene043576:CDS
GTATATCAAGATTCACAGAG+AGG 0.716369 2.2:-7263218 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTACTTACTTTTATTA+AGG + chr2.2:7262436-7262455 MS.gene043576:intron 10.0%
!! AAAAAATAGTAAAAATCTCT+AGG + chr2.2:7260698-7260717 MS.gene043576:intron 15.0%
!! AAATTAATAGATAAAGATGA+GGG + chr2.2:7260909-7260928 MS.gene043576:intron 15.0%
!! TAAGTTATAAGAATCAAATA+TGG + chr2.2:7261926-7261945 MS.gene043576:intron 15.0%
!! TCTAAATTATTAAGCTTAAT+TGG - chr2.2:7261106-7261125 None:intergenic 15.0%
!! TTATTCAATGAATCTAAAAA+AGG + chr2.2:7260109-7260128 MS.gene043576:intron 15.0%
!!! GAAATAAGAAATTTTATACT+AGG + chr2.2:7260209-7260228 MS.gene043576:intron 15.0%
!!! TAAGAAAAGATTCTTTTTTT+AGG + chr2.2:7260035-7260054 MS.gene043576:intron 15.0%
!!! TACTTTTATTAAGGAAATAA+TGG + chr2.2:7262445-7262464 MS.gene043576:intron 15.0%
!! AAGAAATCAAATGTATGATA+TGG - chr2.2:7261082-7261101 None:intergenic 20.0%
!! AATTTCAAGCTATTAGAATT+TGG - chr2.2:7262311-7262330 None:intergenic 20.0%
!! ACTCAAATAATATTATGCTA+TGG - chr2.2:7260378-7260397 None:intergenic 20.0%
!! ATAGCATAATATTATTTGAG+TGG + chr2.2:7260377-7260396 MS.gene043576:intron 20.0%
!! ATTGATATCAACATTAACAT+CGG - chr2.2:7260593-7260612 None:intergenic 20.0%
!! ATTTCAAGCTATTAGAATTT+GGG - chr2.2:7262310-7262329 None:intergenic 20.0%
!! GAAATTAATAGATAAAGATG+AGG + chr2.2:7260908-7260927 MS.gene043576:intron 20.0%
!!! ATTTTAATCAGCACATAAAT+AGG + chr2.2:7260995-7261014 MS.gene043576:intron 20.0%
!!! TATGGTATTGATTTAATACT+TGG + chr2.2:7262943-7262962 MS.gene043576:CDS 20.0%
!!! TGATAATGTGTTTATAAAGT+GGG + chr2.2:7262234-7262253 MS.gene043576:intron 20.0%
! AAAGCAAGAAATCTCTATAA+AGG - chr2.2:7259907-7259926 None:intergenic 25.0%
! AAGAAGTCAAAAATATGGAA+AGG + chr2.2:7262550-7262569 MS.gene043576:CDS 25.0%
! AAGCAAGAAATCTCTATAAA+GGG - chr2.2:7259906-7259925 None:intergenic 25.0%
! AGATCTTACAGAATCAAATA+TGG - chr2.2:7261378-7261397 None:intergenic 25.0%
! AGGAAGAAGAAAAAAAAAAG+AGG + chr2.2:7262049-7262068 MS.gene043576:intron 25.0%
! ATAATGTGTTTATAAAGTGG+GGG + chr2.2:7262236-7262255 MS.gene043576:intron 25.0%
! ATCAATAGTGTCATATCTTA+CGG + chr2.2:7260772-7260791 MS.gene043576:intron 25.0%
! CACTTTATAAACACATTATC+AGG - chr2.2:7262235-7262254 None:intergenic 25.0%
! GATAATGTGTTTATAAAGTG+GGG + chr2.2:7262235-7262254 MS.gene043576:intron 25.0%
! GGAAGAAGAAAAAAAAAAGA+GGG + chr2.2:7262050-7262069 MS.gene043576:intron 25.0%
! GTTAATGTTGATATCAATGT+TGG + chr2.2:7260595-7260614 MS.gene043576:intron 25.0%
! TCTGATAGTAACTTTATGTA+TGG + chr2.2:7262737-7262756 MS.gene043576:intron 25.0%
! TGAAGTAGGTAAACTTATTT+AGG + chr2.2:7260855-7260874 MS.gene043576:intron 25.0%
! TGTTAAAATGAATCCTAAAG+TGG - chr2.2:7263166-7263185 None:intergenic 25.0%
! TTAACACTTGAAATCATCAA+GGG + chr2.2:7263180-7263199 MS.gene043576:CDS 25.0%
! TTCAAATCAAAAGCAGAAAA+CGG - chr2.2:7259737-7259756 None:intergenic 25.0%
! TTGATATCAACATTAACATC+GGG - chr2.2:7260592-7260611 None:intergenic 25.0%
! TTTAACACTTGAAATCATCA+AGG + chr2.2:7263179-7263198 MS.gene043576:CDS 25.0%
! TTTCAAGCTATTAGAATTTG+GGG - chr2.2:7262309-7262328 None:intergenic 25.0%
!! AATGTATGTTTTCATGAAGT+AGG + chr2.2:7260841-7260860 MS.gene043576:intron 25.0%
!! CTGATAATGTGTTTATAAAG+TGG + chr2.2:7262233-7262252 MS.gene043576:intron 25.0%
!! GAAATTTTATACTAGGTACT+AGG + chr2.2:7260216-7260235 MS.gene043576:intron 25.0%
!! GTAACTTTGTACTACATTTT+GGG + chr2.2:7262769-7262788 MS.gene043576:intron 25.0%
!! TCTTTTACTACTGTTACTAA+TGG + chr2.2:7262824-7262843 MS.gene043576:intron 25.0%
!! TGTAACTTTGTACTACATTT+TGG + chr2.2:7262768-7262787 MS.gene043576:intron 25.0%
!! TTTTGTCTCTTCACTAAATA+TGG - chr2.2:7262898-7262917 None:intergenic 25.0%
!!! CAAAGTTCTTTGGTTTTATA+GGG + chr2.2:7259942-7259961 MS.gene043576:intron 25.0%
!!! GTGCTGATTAAAATTTCAAA+AGG - chr2.2:7260989-7261008 None:intergenic 25.0%
!!! TATAACCAAGTACCATTTTT+GGG - chr2.2:7262638-7262657 None:intergenic 25.0%
!!! TATTGTTTTTTGTGTTGTGT+AGG + chr2.2:7259778-7259797 MS.gene043576:intron 25.0%
!!! TCTTTTCTTATATTTGAGAC+CGG + chr2.2:7260134-7260153 MS.gene043576:intron 25.0%
!!! TCTTTTCTTATATTTGAGAC+CGG - chr2.2:7260153-7260134 None:intergenic 25.0%
AAATATGGAAGATGCTGAAA+CGG - chr2.2:7261761-7261742 None:intergenic 30.0%
AACTATGTCTACAATGATTG+TGG + chr2.2:7262083-7262102 MS.gene043576:intron 30.0%
AATCAGCACATAAATAGGTA+TGG + chr2.2:7261000-7261019 MS.gene043576:intron 30.0%
ACAAAAGGTTGTTCATGTAT+CGG - chr2.2:7260658-7260677 None:intergenic 30.0%
AGCTACAAAATTTGTATGCT+TGG - chr2.2:7262712-7262731 None:intergenic 30.0%
AGTACTTTCAAAACTCAAGA+TGG - chr2.2:7261034-7261053 None:intergenic 30.0%
ATAAGAATCAAATATGGACG+AGG + chr2.2:7261932-7261951 MS.gene043576:intron 30.0%
ATCATTGTAGACATAGTTGT+TGG - chr2.2:7262081-7262100 None:intergenic 30.0%
ATGTTGATATCAATGTTGGT+AGG + chr2.2:7260599-7260618 MS.gene043576:intron 30.0%
CAAGCAAGAAGTCAAAAATA+TGG + chr2.2:7262545-7262564 MS.gene043576:CDS 30.0%
CATTGAGTAGTTAATGTATC+TGG + chr2.2:7260060-7260079 MS.gene043576:intron 30.0%
GATTTAATACTTGGCTGTAA+TGG + chr2.2:7262952-7262971 MS.gene043576:CDS 30.0%
GGTCTTACAAGAATCAAATA+TGG + chr2.2:7261793-7261812 MS.gene043576:intron 30.0%
TAAATTATGTACATGTCCAC+AGG + chr2.2:7262481-7262500 MS.gene043576:intron 30.0%
TATGTCTACAATGATTGTAG+CGG - chr2.2:7261158-7261177 None:intergenic 30.0%
TGCCAATTGCAAAACAATTA+TGG + chr2.2:7260939-7260958 MS.gene043576:intron 30.0%
TGTGGACATGTACATAATTT+AGG - chr2.2:7262482-7262501 None:intergenic 30.0%
TTATGTTTCACAAGTGTAAG+AGG + chr2.2:7260242-7260261 MS.gene043576:intron 30.0%
TTATTGGGAATTAAGACTTG+TGG - chr2.2:7263048-7263067 None:intergenic 30.0%
TTCTAAATAAATACTCCCTC+CGG + chr2.2:7260003-7260022 MS.gene043576:intron 30.0%
! AAGACTGACAGAACTTTTAT+TGG - chr2.2:7263064-7263083 None:intergenic 30.0%
! AAGCAGTTCATAATGAGTTT+TGG + chr2.2:7260464-7260483 MS.gene043576:intron 30.0%
! AATCGAGAAAACTTTTCTGA+AGG + chr2.2:7261772-7261791 MS.gene043576:intron 30.0%
! AGACTGACAGAACTTTTATT+GGG - chr2.2:7263063-7263082 None:intergenic 30.0%
! GCATACAAATTTTGTAGCTA+TGG + chr2.2:7262713-7262732 MS.gene043576:intron 30.0%
! GTATAACCAAGTACCATTTT+TGG - chr2.2:7262639-7262658 None:intergenic 30.0%
! TGTTGGTTGTGAAACAAATA+TGG + chr2.2:7261727-7261746 MS.gene043576:intron 30.0%
! TGTTGGTTGTGAAACAAATA+TGG - chr2.2:7261746-7261727 None:intergenic 30.0%
! TTGTTGTTACATTCTGTTCT+GGG - chr2.2:7261488-7261507 None:intergenic 30.0%
!! AAATATGCGCTGTATTTTTG+AGG + chr2.2:7262374-7262393 MS.gene043576:CDS 30.0%
!! ATAACCAAGTACCATTTTTG+GGG - chr2.2:7262637-7262656 None:intergenic 30.0%
!! GACCATAATTGTTTTGCAAT+TGG - chr2.2:7260944-7260963 None:intergenic 30.0%
!! GCAAACTATTCTGTTTTTCT+TGG - chr2.2:7260276-7260295 None:intergenic 30.0%
!! TGTGTCAACATTTGATTTGA+TGG + chr2.2:7260813-7260832 MS.gene043576:intron 30.0%
!! TTAAGACTTGTGGATTAGAT+TGG - chr2.2:7263038-7263057 None:intergenic 30.0%
!! TTTGTTGTTACATTCTGTTC+TGG - chr2.2:7261489-7261508 None:intergenic 30.0%
!!! ATGCTTTTTTTCTGCAATCT+GGG + chr2.2:7260727-7260746 MS.gene043576:intron 30.0%
!!! ATTTTTGACTTCTTGCTTGA+AGG - chr2.2:7262544-7262563 None:intergenic 30.0%
!!! CTTATTTTTTCCCCCAAAAA+TGG + chr2.2:7262623-7262642 MS.gene043576:intron 30.0%
!!! GCAAAGTTCTTTGGTTTTAT+AGG + chr2.2:7259941-7259960 MS.gene043576:intron 30.0%
AAACCAACATTTCCATCATC+TGG + chr2.2:7262915-7262934 MS.gene043576:CDS 35.0%
AATAGTGTCATATCTTACGG+AGG + chr2.2:7260775-7260794 MS.gene043576:intron 35.0%
ACAAGAATCAAATATGGACG+AGG + chr2.2:7261799-7261818 MS.gene043576:intron 35.0%
AGACAAGTGAATACATTTGC+AGG + chr2.2:7263115-7263134 MS.gene043576:CDS 35.0%
AGAGAAAAACTATACTGCAC+TGG + chr2.2:7263092-7263111 MS.gene043576:CDS 35.0%
AGTAATCCACGAAAAGTTAC+AGG + chr2.2:7260161-7260180 MS.gene043576:intron 35.0%
ATCATCGTAGACATAGTTGT+TGG - chr2.2:7262012-7262031 None:intergenic 35.0%
ATCTTCTATCATGTCATCTC+TGG - chr2.2:7263007-7263026 None:intergenic 35.0%
ATCTTCTGATTCTTCTGTGA+CGG + chr2.2:7259495-7259514 MS.gene043576:CDS 35.0%
ATGTAGTCAGCAAAGTTCTT+TGG + chr2.2:7259932-7259951 MS.gene043576:intron 35.0%
ATTGTTCTTTCCATTGCTAG+AGG + chr2.2:7262328-7262347 MS.gene043576:intron 35.0%
CTGAAAATCACGAACTACTT+CGG - chr2.2:7262507-7262526 None:intergenic 35.0%
GACAAGTGAATACATTTGCA+GGG + chr2.2:7263116-7263135 MS.gene043576:CDS 35.0%
GATACGATGTAACGACATTA+AGG + chr2.2:7261549-7261568 MS.gene043576:intron 35.0%
GTAACAACAAATTCACAGCA+AGG + chr2.2:7261498-7261517 MS.gene043576:intron 35.0%
GTAATCCACGAAAAGTTACA+GGG + chr2.2:7260162-7260181 MS.gene043576:intron 35.0%
GTATATCAAGATTCACAGAG+AGG - chr2.2:7263221-7263240 None:intergenic 35.0%
TACAGAATCAAATATGGACG+AGG - chr2.2:7261372-7261391 None:intergenic 35.0%
TATTTACACGCTGTATGTGT+TGG + chr2.2:7261710-7261729 MS.gene043576:intron 35.0%
TGTTGTTACATTCTGTTCTG+GGG - chr2.2:7261487-7261506 None:intergenic 35.0%
! AAGCTATTAGAATTTGGGGT+TGG - chr2.2:7262305-7262324 None:intergenic 35.0%
! AAGTAGCCTTACAATGAACT+CGG + chr2.2:7261842-7261861 MS.gene043576:intron 35.0%
! AGCTATTAGAATTTGGGGTT+GGG - chr2.2:7262304-7262323 None:intergenic 35.0%
! ATTCGTAGTGAATCGATTCT+AGG + chr2.2:7260487-7260506 MS.gene043576:intron 35.0%
! GATACATGAACAACCTTTTG+TGG + chr2.2:7260657-7260676 MS.gene043576:intron 35.0%
! GATTGTGTCTTTTCTTCAAC+AGG + chr2.2:7262029-7262048 MS.gene043576:intron 35.0%
! TTCAATGCTTTTCTTCACCA+AGG + chr2.2:7261283-7261302 MS.gene043576:intron 35.0%
! TTCTTATATTTGAGACCGGA+GGG + chr2.2:7260138-7260157 MS.gene043576:intron 35.0%
! TTCTTATATTTGAGACCGGA+GGG - chr2.2:7260157-7260138 None:intergenic 35.0%
! TTTCTTATATTTGAGACCGG+AGG + chr2.2:7260137-7260156 MS.gene043576:intron 35.0%
! TTTCTTATATTTGAGACCGG+AGG - chr2.2:7260156-7260137 None:intergenic 35.0%
!! GTTAGAGTATTGTCCACTTT+AGG + chr2.2:7263150-7263169 MS.gene043576:CDS 35.0%
!! TAACCAAGTACCATTTTTGG+GGG - chr2.2:7262636-7262655 None:intergenic 35.0%
!! TGGCTGTAATGGATTCATAT+GGG + chr2.2:7262963-7262982 MS.gene043576:CDS 35.0%
!! TGTAATGGATTCATATGGGT+CGG + chr2.2:7262967-7262986 MS.gene043576:CDS 35.0%
!! TTGGCTGTAATGGATTCATA+TGG + chr2.2:7262962-7262981 MS.gene043576:CDS 35.0%
!! TTTTTCAACCTTTGATTCGC+AGG - chr2.2:7261635-7261654 None:intergenic 35.0%
!!! GAGTTTTGAAAGTACTCGAT+TGG + chr2.2:7261038-7261057 MS.gene043576:intron 35.0%
!!! GATGCTTTTTTTCTGCAATC+TGG + chr2.2:7260726-7260745 MS.gene043576:intron 35.0%
!!! GTTTTTTGTGTTGTGTAGGT+TGG + chr2.2:7259782-7259801 MS.gene043576:intron 35.0%
AAAGGTTGTTCATGTATCGG+AGG - chr2.2:7260655-7260674 None:intergenic 40.0%
AAATCCCCAAATATGTCCCA+AGG - chr2.2:7260540-7260559 None:intergenic 40.0%
AAGGCCTATCCAAGATCTAT+TGG + chr2.2:7262133-7262152 MS.gene043576:intron 40.0%
AAGGCTGGTGACATTGTTAT+AGG + chr2.2:7259673-7259692 MS.gene043576:CDS 40.0%
AAGGTTGCTTCCAATGAACT+CGG - chr2.2:7261338-7261357 None:intergenic 40.0%
ACATCGTATCTGTGAATCAG+AGG - chr2.2:7261539-7261558 None:intergenic 40.0%
ACTTTGTACCTGCGAATCAA+AGG + chr2.2:7261624-7261643 MS.gene043576:intron 40.0%
AGGCCTATCCAAGATCTATT+GGG + chr2.2:7262134-7262153 MS.gene043576:intron 40.0%
AGGCTGCAATTGTAGAAGAT+GGG + chr2.2:7260507-7260526 MS.gene043576:intron 40.0%
AGGCTGGTGACATTGTTATA+GGG + chr2.2:7259674-7259693 MS.gene043576:CDS 40.0%
ATAACAATGTCACCAGCCTT+GGG - chr2.2:7259673-7259692 None:intergenic 40.0%
ATATTTCCAGCAGTAGGTGT+TGG - chr2.2:7261460-7261479 None:intergenic 40.0%
ATCATGTGCATCACCACAAA+AGG - chr2.2:7260673-7260692 None:intergenic 40.0%
ATGGAAGATGCTGAAACGAA+AGG + chr2.2:7261746-7261765 MS.gene043576:intron 40.0%
CAAAATACCTGTAGACCATC+GGG - chr2.2:7259878-7259897 None:intergenic 40.0%
CCAGTATGAGACAGTACAAA+TGG + chr2.2:7262666-7262685 MS.gene043576:intron 40.0%
CCATTTGTACTGTCTCATAC+TGG - chr2.2:7262669-7262688 None:intergenic 40.0%
CCTTACAAAGTATGCTGGTA+AGG - chr2.2:7262271-7262290 None:intergenic 40.0%
CCTTACCAGCATACTTTGTA+AGG + chr2.2:7262268-7262287 MS.gene043576:intron 40.0%
CTCAACCTTACAAAGTATGC+TGG - chr2.2:7262276-7262295 None:intergenic 40.0%
CTTGATATACACGACATGCT+TGG + chr2.2:7263231-7263250 MS.gene043576:CDS 40.0%
GCAAAATACCTGTAGACCAT+CGG - chr2.2:7259879-7259898 None:intergenic 40.0%
GCAGCAACAAGAACAACAAA+AGG - chr2.2:7261246-7261265 None:intergenic 40.0%
GTCTCACGTTGGATAAGATA+TGG + chr2.2:7262209-7262228 MS.gene043576:intron 40.0%
GTTGTATTGCAGCTTAGTAC+TGG + chr2.2:7262853-7262872 MS.gene043576:intron 40.0%
TAGGCTGCAATTGTAGAAGA+TGG + chr2.2:7260506-7260525 MS.gene043576:intron 40.0%
TATAACAATGTCACCAGCCT+TGG - chr2.2:7259674-7259693 None:intergenic 40.0%
TGAAACGGAAGGAAGAATTG+AGG - chr2.2:7261413-7261432 None:intergenic 40.0%
TGTATGCTTGGATCACAAAC+AGG - chr2.2:7262700-7262719 None:intergenic 40.0%
TTATTGCAAGCTTGGCATCA+AGG + chr2.2:7262114-7262133 MS.gene043576:intron 40.0%
TTGAAACCGTGACAAAGTGA+CGG - chr2.2:7261586-7261605 None:intergenic 40.0%
TTGCAGAAAAAGAGGCAGAA+AGG + chr2.2:7263274-7263293 MS.gene043576:CDS 40.0%
TTGCCCAATAGATCTTGGAT+AGG - chr2.2:7262140-7262159 None:intergenic 40.0%
TTTCCCCCAAAAATGGTACT+TGG + chr2.2:7262630-7262649 MS.gene043576:intron 40.0%
! AAAGACCCTGTAACTTTTCG+TGG - chr2.2:7260170-7260189 None:intergenic 40.0%
! AGTAGCCTTACAATGAACTC+GGG + chr2.2:7261843-7261862 MS.gene043576:intron 40.0%
! GGGACATATTTGGGGATTTT+GGG + chr2.2:7260541-7260560 MS.gene043576:intron 40.0%
! GTTCGTGATTTTCAGCATGA+TGG + chr2.2:7262512-7262531 MS.gene043576:CDS 40.0%
! TGGGACATATTTGGGGATTT+TGG + chr2.2:7260540-7260559 MS.gene043576:intron 40.0%
! TGTGCACTTCGTTCAGTTTT+GGG + chr2.2:7261681-7261700 MS.gene043576:intron 40.0%
! TGTTCTGGTTGCAGAAAAAG+AGG + chr2.2:7263266-7263285 MS.gene043576:CDS 40.0%
! TTGTGCACTTCGTTCAGTTT+TGG + chr2.2:7261680-7261699 MS.gene043576:intron 40.0%
!! ATCTTCATGGTTGATAGGGA+TGG - chr2.2:7259530-7259549 None:intergenic 40.0%
!! CAGTCTTGTTATTGCAAGCT+TGG + chr2.2:7262106-7262125 MS.gene043576:intron 40.0%
!! TATGCGCTGTATTTTTGAGG+AGG + chr2.2:7262377-7262396 MS.gene043576:CDS 40.0%
AAAGCCATGGTGAAGCAAAC+AGG + chr2.2:7261881-7261900 MS.gene043576:intron 45.0%
AACTTCGCCGTTGATATCAG+CGG - chr2.2:7259575-7259594 None:intergenic 45.0%
AAGCCATGGTGAAGCAAACA+GGG + chr2.2:7261882-7261901 MS.gene043576:intron 45.0%
ACAGTGCCTTGGGACATATT+TGG + chr2.2:7260531-7260550 MS.gene043576:intron 45.0%
ACGCGATCTTCATGGTTGAT+AGG - chr2.2:7259535-7259554 None:intergenic 45.0%
AGTGCCTTGGGACATATTTG+GGG + chr2.2:7260533-7260552 MS.gene043576:intron 45.0%
ATGGAAGATGCTGAAACGGA+AGG - chr2.2:7261424-7261443 None:intergenic 45.0%
CAAAGTATGCTGGTAAGGTG+AGG - chr2.2:7262266-7262285 None:intergenic 45.0%
CAGTGCCTTGGGACATATTT+GGG + chr2.2:7260532-7260551 MS.gene043576:intron 45.0%
CATGAAGATCGCGTACTCAA+AGG + chr2.2:7259541-7259560 MS.gene043576:CDS 45.0%
CATGGAACCGCTGATATCAA+CGG + chr2.2:7259565-7259584 MS.gene043576:CDS 45.0%
CTAGAGCATGACCTAATAGC+AGG - chr2.2:7262163-7262182 None:intergenic 45.0%
CTATTGGGCAACCTGCTATT+AGG + chr2.2:7262149-7262168 MS.gene043576:intron 45.0%
CTTTGAGTACGCGATCTTCA+TGG - chr2.2:7259543-7259562 None:intergenic 45.0%
GAAGTTGTTGCTACTCTCTG+TGG + chr2.2:7259589-7259608 MS.gene043576:CDS 45.0%
GAGTTCATTGGAAGCAACCT+TGG + chr2.2:7261337-7261356 MS.gene043576:intron 45.0%
GTCATATCTTACGGAGGCTA+GGG + chr2.2:7260781-7260800 MS.gene043576:intron 45.0%
TACCATACTGCTCCAGATGA+TGG - chr2.2:7262930-7262949 None:intergenic 45.0%
TCAAGATACAAACCCAAGGC+TGG + chr2.2:7259658-7259677 MS.gene043576:CDS 45.0%
TCAGAAGATCCGTCTACAGA+AGG + chr2.2:7259450-7259469 MS.gene043576:CDS 45.0%
TCATATCTTACGGAGGCTAG+GGG + chr2.2:7260782-7260801 MS.gene043576:intron 45.0%
TCTGCTATGAATATGCCCGA+TGG + chr2.2:7259860-7259879 MS.gene043576:CDS 45.0%
TCTTCAACAGCAGCTCAAAC+AGG - chr2.2:7261207-7261226 None:intergenic 45.0%
TGCAACCAGAACACAGAACT+CGG - chr2.2:7263259-7263278 None:intergenic 45.0%
TGGTCAGAATCGTTGGAGAT+TGG + chr2.2:7259802-7259821 MS.gene043576:CDS 45.0%
TGTCATATCTTACGGAGGCT+AGG + chr2.2:7260780-7260799 MS.gene043576:intron 45.0%
TGTGTTGAAGAGTCTCACGT+TGG + chr2.2:7262198-7262217 MS.gene043576:intron 45.0%
TTGGGAAGCTCTTAGTTGCA+GGG + chr2.2:7260559-7260578 MS.gene043576:intron 45.0%
TTTCCCTGTTTGCTTCACCA+TGG - chr2.2:7261888-7261907 None:intergenic 45.0%
TTTGGGAAGCTCTTAGTTGC+AGG + chr2.2:7260558-7260577 MS.gene043576:intron 45.0%
! CGCGATCTTCATGGTTGATA+GGG - chr2.2:7259534-7259553 None:intergenic 45.0%
! CTGAAACCGTCACTTTGTCA+CGG + chr2.2:7261577-7261596 MS.gene043576:intron 45.0%
! CTGGGACTGATTGGGTTTTT+GGG + chr2.2:7260745-7260764 MS.gene043576:intron 45.0%
! GTAAGGCTACTTTCGAACCT+TGG - chr2.2:7261834-7261853 None:intergenic 45.0%
! GTGCACTTCGTTCAGTTTTG+GGG + chr2.2:7261682-7261701 MS.gene043576:intron 45.0%
! TCTGCAATCTGGGACTGATT+GGG + chr2.2:7260737-7260756 MS.gene043576:intron 45.0%
! TGGGACTGATTGGGTTTTTG+GGG + chr2.2:7260746-7260765 MS.gene043576:intron 45.0%
! TTCCATCATCTGGAGCAGTA+TGG + chr2.2:7262925-7262944 MS.gene043576:CDS 45.0%
! TTCTGCAATCTGGGACTGAT+TGG + chr2.2:7260736-7260755 MS.gene043576:intron 45.0%
!! CTTTGAAGTGAGGCTGAGAT+AGG + chr2.2:7261306-7261325 MS.gene043576:intron 45.0%
!! GCTCCAGATGATGGAAATGT+TGG - chr2.2:7262921-7262940 None:intergenic 45.0%
!! TCTGGGACTGATTGGGTTTT+TGG + chr2.2:7260744-7260763 MS.gene043576:intron 45.0%
!! TGTAGGTTGGTCAGAATCGT+TGG + chr2.2:7259795-7259814 MS.gene043576:intron 45.0%
!! TTCACCAAGGCTTTGAAGTG+AGG + chr2.2:7261296-7261315 MS.gene043576:intron 45.0%
!! TTCACCATGGCTTTGAAGTG+AGG - chr2.2:7261875-7261894 None:intergenic 45.0%
!! TTTTCGTGGATTACTCCCTC+CGG - chr2.2:7260156-7260175 None:intergenic 45.0%
AGAAGATGGGAACAGTGCCT+TGG + chr2.2:7260520-7260539 MS.gene043576:intron 50.0%
AGGATCGCGAGTTACCTTGT+GGG + chr2.2:7260619-7260638 MS.gene043576:intron 50.0%
AGTGAGGCTGAGATAGGTTG+AGG + chr2.2:7261312-7261331 MS.gene043576:intron 50.0%
CAACAGCAGCTCAAACAGGA+GGG - chr2.2:7261203-7261222 None:intergenic 50.0%
CTTTCCATTGCTAGAGGCGA+AGG + chr2.2:7262334-7262353 MS.gene043576:intron 50.0%
GAAGATGGGAACAGTGCCTT+GGG + chr2.2:7260521-7260540 MS.gene043576:intron 50.0%
GAATATGCCCGATGGTCTAC+AGG + chr2.2:7259868-7259887 MS.gene043576:CDS 50.0%
GAGGATCAAGCAATCCCACA+AGG - chr2.2:7260636-7260655 None:intergenic 50.0%
GATCGCGTACTCAAAGGACA+TGG + chr2.2:7259547-7259566 MS.gene043576:CDS 50.0%
GCAGGTTGCCCAATAGATCT+TGG - chr2.2:7262145-7262164 None:intergenic 50.0%
GCGCTCAAGATACAAACCCA+AGG + chr2.2:7259654-7259673 MS.gene043576:CDS 50.0%
GGCTGCATATTTCCAGCAGT+AGG - chr2.2:7261466-7261485 None:intergenic 50.0%
GTTCCAGTGCCTTCTGTAGA+CGG - chr2.2:7259462-7259481 None:intergenic 50.0%
TAGGATCGCGAGTTACCTTG+TGG + chr2.2:7260618-7260637 MS.gene043576:intron 50.0%
TCAACAGCAGCTCAAACAGG+AGG - chr2.2:7261204-7261223 None:intergenic 50.0%
TCACAACCAACACCTACTGC+TGG + chr2.2:7261451-7261470 MS.gene043576:intron 50.0%
TCAGCCTCACTTCAAAGCCA+TGG + chr2.2:7261868-7261887 MS.gene043576:intron 50.0%
TCAGCCTCACTTCAAAGCCT+TGG - chr2.2:7261303-7261322 None:intergenic 50.0%
TGAGACTCTTCAACACACGC+AGG - chr2.2:7262195-7262214 None:intergenic 50.0%
TGAGGCCCGAGTTCATTGTA+AGG - chr2.2:7261851-7261870 None:intergenic 50.0%
TGGTTCCGAGTTCTGTGTTC+TGG + chr2.2:7263251-7263270 MS.gene043576:CDS 50.0%
! TAGGTTGAGGCCGAGTTCAT+TGG + chr2.2:7261325-7261344 MS.gene043576:intron 50.0%
! TGCTACTCTCTGTGGTGTTG+TGG + chr2.2:7259597-7259616 MS.gene043576:CDS 50.0%
!! ATGGCTTTGAAGTGAGGCTG+AGG - chr2.2:7261869-7261888 None:intergenic 50.0%
!! GGACTGATTGGGTTTTTGGG+GGG + chr2.2:7260748-7260767 MS.gene043576:intron 50.0%
!! GGGACTGATTGGGTTTTTGG+GGG + chr2.2:7260747-7260766 MS.gene043576:intron 50.0%
CTCTTCAACACACGCAGGAC+TGG - chr2.2:7262190-7262209 None:intergenic 55.0%
CTGTCCTTCGCCTCTAGCAA+TGG - chr2.2:7262341-7262360 None:intergenic 55.0%
GGACGAGGCTACAGAATCCA+AGG + chr2.2:7261814-7261833 MS.gene043576:intron 55.0%
GGACGAGGCTACATGATCCA+AGG - chr2.2:7261357-7261376 None:intergenic 55.0%
TCACGAACTACTTCGGCCTG+TGG - chr2.2:7262500-7262519 None:intergenic 55.0%
! GATCCGTCTACAGAAGGCAC+TGG + chr2.2:7259456-7259475 MS.gene043576:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 7259418 7263320 7259418 ID=MS.gene043576
chr2.2 mRNA 7259418 7263320 7259418 ID=MS.gene043576.t1;Parent=MS.gene043576
chr2.2 exon 7259418 7259708 7259418 ID=MS.gene043576.t1.exon1;Parent=MS.gene043576.t1
chr2.2 CDS 7259418 7259708 7259418 ID=cds.MS.gene043576.t1;Parent=MS.gene043576.t1
chr2.2 exon 7259800 7259889 7259800 ID=MS.gene043576.t1.exon2;Parent=MS.gene043576.t1
chr2.2 CDS 7259800 7259889 7259800 ID=cds.MS.gene043576.t1;Parent=MS.gene043576.t1
chr2.2 exon 7262348 7262413 7262348 ID=MS.gene043576.t1.exon3;Parent=MS.gene043576.t1
chr2.2 CDS 7262348 7262413 7262348 ID=cds.MS.gene043576.t1;Parent=MS.gene043576.t1
chr2.2 exon 7262503 7262571 7262503 ID=MS.gene043576.t1.exon4;Parent=MS.gene043576.t1
chr2.2 CDS 7262503 7262571 7262503 ID=cds.MS.gene043576.t1;Parent=MS.gene043576.t1
chr2.2 exon 7262865 7263320 7262865 ID=MS.gene043576.t1.exon5;Parent=MS.gene043576.t1
chr2.2 CDS 7262865 7263320 7262865 ID=cds.MS.gene043576.t1;Parent=MS.gene043576.t1
Gene Sequence

>MS.gene043576

ATGAGAAGGATTCAGTTTCAATTGAGTCAAACTCAGAAGATCCGTCTACAGAAGGCACTGGAACATCTCGAATCTCTATCTTCTGATTCTTCTGTGACGGTATCTGATTCCATCCCTATCAACCATGAAGATCGCGTACTCAAAGGACATGGAACCGCTGATATCAACGGCGAAGTTGTTGCTACTCTCTGTGGTGTTGTGGAGCGTGTTGACAAGTATGTTTGTGTGCGTGCTTTGCGCTCAAGATACAAACCCAAGGCTGGTGACATTGTTATAGGGCGTGTCATTGACGTAAAGTTTTATAGTTTCAATTCAACCGTTTTCTGCTTTTGATTTGAAAATGTTTCAATCGAGTAATCTTATTGTTTTTTGTGTTGTGTAGGTTGGTCAGAATCGTTGGAGATTGGATATTAATTGCAGTCAAAATGCTTATATGATGCTTTCTGCTATGAATATGCCCGATGGTCTACAGGTATTTTGCAACTCCCTTTATAGAGATTTCTTGCTTTTCATTATGTAGTCAGCAAAGTTCTTTGGTTTTATAGGGCTCTTTATCTGTGTTTGTTTGAAGAAAAGTTAGAGTCTTTCTAAATAAATACTCCCTCCGGTCTCAAATATAAGAAAAGATTCTTTTTTTAGGTTCATTGAGTAGTTAATGTATCTGGCATACATTGTAAAGCAAATACATTAATTATTCAATGAATCTAAAAAAGGAATCTTTTCTTATATTTGAGACCGGAGGGAGTAATCCACGAAAAGTTACAGGGTCTTTCTGAATAAATAATCTTTAAGAAATAAGAAATTTTATACTAGGTACTAGGTAGTTATGTTTCACAAGTGTAAGAGGTGAAACATCCAAGAAAAACAGAATAGTTTGCAGATAACAATTTTAACAATGTTTTCACGACTATGTGTAATAGAATATGAAGAATTGATTTGTTTTTTGTCGCTTTTTTGCCATAGCATAATATTATTTGAGTGGATAAAATGATGAACAAGAGTCATGTATGTATTTAGTAGTTTTATATAAAATAGTATTTGAGTATAAGCAGTTCATAATGAGTTTTGGATTCGTAGTGAATCGATTCTAGGCTGCAATTGTAGAAGATGGGAACAGTGCCTTGGGACATATTTGGGGATTTTGGGAAGCTCTTAGTTGCAGGGCAAGCTTCCCGATGTTAATGTTGATATCAATGTTGGTAGGATCGCGAGTTACCTTGTGGGATTGCTTGATCCTCCGATACATGAACAACCTTTTGTGGTGATGCACATGATTGCTGAAAAAATAGTAAAAATCTCTAGGATAGTGATGCTTTTTTTCTGCAATCTGGGACTGATTGGGTTTTTGGGGGGAATCAATAGTGTCATATCTTACGGAGGCTAGGGGTCACAACTTGTGTCAACATTTGATTTGATGGTCATCAATGTATGTTTTCATGAAGTAGGTAAACTTATTTAGGTGTAACTTTTTATATACTTTAAATTTCAAAGAAATTAATAGATAAAGATGAGGGTTACAAATGCCAATTGCAAAACAATTATGGTCATCACTTATCAAGTAGTCAAGTCCTTTTGAAATTTTAATCAGCACATAAATAGGTATGGTGCACAAACCATCTTGAGTTTTGAAAGTACTCGATTGGTTTTCTTGACTTTACATACCATATCATACATTTGATTTCTTTCCAATTAAGCTTAATAATTTAGAATAAATAGCTTAGCTTGCAGATGTCAAGACCGCTACAATCATTGTAGACATAGCTGTTGTTGTTGTTGAAAACTCCCTCCTGTTTGAGCTGCTGTTGAAGAATACATTTGATTTCTTCCTTTTGTTGTTCTTGTTGCTGCTTCAGTTGAAGAAAATCTTCAATGCTTTTCTTCACCAAGGCTTTGAAGTGAGGCTGAGATAGGTTGAGGCCGAGTTCATTGGAAGCAACCTTGGATCATGTAGCCTCGTCCATATTTGATTCTGTAAGATCTTCAGAACAGTTTCCTCAATTCTTCCTTCCGTTTCAGCATCTTCCATATTTGTTTCACAACCAACACCTACTGCTGGAAATATGCAGCCCCAGAACAGAATGTAACAACAAATTCACAGCAAGGTCTTATTTTTCTGCACCTCTGATTCACAGATACGATGTAACGACATTAAGGTGTATCTGAAACCGTCACTTTGTCACGGTTTCAAATACACGTTAACGTTGTTACTTTGTACCTGCGAATCAAAGGTTGAAAAATCAAGAACTTGTTGTCGACAATTTTTTGTGCACTTCGTTCAGTTTTGGGGATGCATATTTACACGCTGTATGTGTTGGTTGTGAAACAAATATGGAAGATGCTGAAACGAAAGGAAGAATCGAGAAAACTTTTCTGAAGGTCTTACAAGAATCAAATATGGACGAGGCTACAGAATCCAAGGTTCGAAAGTAGCCTTACAATGAACTCGGGCCTCAGCCTCACTTCAAAGCCATGGTGAAGCAAACAGGGAAAACTGTTCTGAAGATCTTATAAGTTATAAGAATCAAATATGGACGAGGCAACAAAATTCAAGATTCACTTTAAAGCTTTTTTTCAACAGCAAGAGTGAGTTTCCAACAACTATGTCTACGATGATTGTGTCTTTTCTTCAACAGGAAGAAGAAAAAAAAAAGAGGGAATTTCCAACAACTATGTCTACAATGATTGTGGCAGTCTTGTTATTGCAAGCTTGGCATCAAGGCCTATCCAAGATCTATTGGGCAACCTGCTATTAGGTCATGCTCTAGATATCCAGTCCTGCGTGTGTTGAAGAGTCTCACGTTGGATAAGATATGGCCTGATAATGTGTTTATAAAGTGGGGGCAATCCTCACCTTACCAGCATACTTTGTAAGGTTGAGTTAGTCCCAACCCCAAATTCTAATAGCTTGAAATTGTTCTTTCCATTGCTAGAGGCGAAGGACAGCTTTAGATGAGCTAAATATGCGCTGTATTTTTGAGGAGGAAGATGTCATTTGTGTAAGTGAAATCATGTTTATTAAAATTTACTTACTTTTATTAAGGAAATAATGGTTGACTTGTATCCTAAATTATGTACATGTCCACAGGCCGAAGTAGTTCGTGATTTTCAGCATGATGGCTTATACCTTCAAGCAAGAAGTCAAAAATATGGAAAGGTTTGCAGTCTTGAAAACTCTGTGAAACATAGTTCTTTTATTGACTCGCTTCTTATTTTTTCCCCCAAAAATGGTACTTGGTTATACATAGTAGCCAGTATGAGACAGTACAAATGGAACAACTTCCTGTTTGTGATCCAAGCATACAAATTTTGTAGCTATGGTTCTGATAGTAACTTTATGTATGGCTTGTAGTTGTAACTTTGTACTACATTTTGGGTTCTTAAAATTTGTTTCAATCATATTCTTCATTCTTTTACTACTGTTACTAATGGCTTTCAGTTGTATTGCAGCTTAGTACTGGTCAACTGCTTACAGTCGCACCATATTTAGTGAAGAGACAAAACCAACATTTCCATCATCTGGAGCAGTATGGTATTGATTTAATACTTGGCTGTAATGGATTCATATGGGTCGGAGAACATGTTGAAGCCAGAGATGACATGATAGAAGATCAAATAAACCAATCTAATCCACAAGTCTTAATTCCCAATAAAAGTTCTGTCAGTCTTGAAGAACAAGAGAAAAACTATACTGCACTGGAGACAAGTGAATACATTTGCAGGGCTGCTAATGCTGTTAGAGTATTGTCCACTTTAGGATTCATTTTAACACTTGAAATCATCAAGGGAGTTATTGATCTAAGCCTCTCTGTGAATCTTGATATACACGACATGCTTGGTTCCGAGTTCTGTGTTCTGGTTGCAGAAAAAGAGGCAGAAAGGAGAAGCTCAAATAAAAATCCGTAG
Protein sequence

>MS.gene043576.t1

MRRIQFQLSQTQKIRLQKALEHLESLSSDSSVTVSDSIPINHEDRVLKGHGTADINGEVVATLCGVVERVDKYVCVRALRSRYKPKAGDIVIGRVIDVGQNRWRLDINCSQNAYMMLSAMNMPDGLQRRRTALDELNMRCIFEEEDVICAEVVRDFQHDGLYLQARSQKYGKLSTGQLLTVAPYLVKRQNQHFHHLEQYGIDLILGCNGFIWVGEHVEARDDMIEDQINQSNPQVLIPNKSSVSLEEQEKNYTALETSEYICRAANAVRVLSTLGFILTLEIIKGVIDLSLSVNLDIHDMLGSEFCVLVAEKEAERRSSNKNP