Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046177.t1 | XP_003592861.2 | 97.5 | 634 | 15 | 1 | 1 | 633 | 1 | 634 | 0.00E+00 | 1224.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046177.t1 | Q9FQ08 | 63.1 | 626 | 218 | 5 | 10 | 633 | 7 | 621 | 5.0e-228 | 792.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046177.t1 | G7ICA9 | 97.5 | 634 | 15 | 1 | 1 | 633 | 1 | 634 | 0.0e+00 | 1224.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene87898 | MS.gene046177 | PPI |
| MS.gene046177 | MS.gene042871 | PPI |
| MS.gene046177 | MS.gene75567 | PPI |
| MS.gene046177 | MS.gene066960 | PPI |
| MS.gene046177 | MS.gene79126 | PPI |
| MS.gene32832 | MS.gene046177 | PPI |
| MS.gene046177 | MS.gene49838 | PPI |
| MS.gene79799 | MS.gene046177 | PPI |
| MS.gene046177 | MS.gene015034 | PPI |
| MS.gene046177 | MS.gene012829 | PPI |
| MS.gene046177 | MS.gene045227 | PPI |
| MS.gene046177 | MS.gene37440 | PPI |
| MS.gene046177 | MS.gene82109 | PPI |
| MS.gene046177 | MS.gene47284 | PPI |
| MS.gene046177 | MS.gene55626 | PPI |
| MS.gene046177 | MS.gene067039 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046177.t1 | MTR_1g116980 | 97.476 | 634 | 15 | 1 | 1 | 633 | 1 | 634 | 0.0 | 1280 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene046177.t1 | AT1G16970 | 63.259 | 626 | 217 | 5 | 10 | 633 | 7 | 621 | 0.0 | 819 |
Find 128 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGGAATCGTTATTATTT+TGG | 0.127811 | 1.3:-79610242 | None:intergenic |
| GGACACAATCTTCCTGTTTC+TGG | 0.163777 | 1.3:+79623174 | MS.gene046177:CDS |
| ATCCTCTCTACAATGCTATC+TGG | 0.229737 | 1.3:+79610714 | MS.gene046177:CDS |
| CATATTGTACCTGCGCAATA+AGG | 0.235695 | 1.3:-79617397 | None:intergenic |
| AATGTTTCATCTTTGATTTC+AGG | 0.249243 | 1.3:-79622246 | None:intergenic |
| GCTTACCAGGGTTGGTGATT+TGG | 0.272499 | 1.3:-79617876 | None:intergenic |
| CAAGTGATGATCAAATAAAA+AGG | 0.279053 | 1.3:+79617806 | MS.gene046177:CDS |
| GCATATGGCAATTTGATCTA+AGG | 0.291855 | 1.3:-79610406 | None:intergenic |
| TTCCCATATGAGTTAATATT+CGG | 0.307537 | 1.3:-79623218 | None:intergenic |
| CATGTCTTTCAGGATTTGAT+TGG | 0.315339 | 1.3:+79611032 | MS.gene046177:intron |
| ACAAATCAAGATGATCCTTT+TGG | 0.319342 | 1.3:+79610800 | MS.gene046177:CDS |
| TATTACTAATCTTCCTTTGA+AGG | 0.323096 | 1.3:+79612485 | MS.gene046177:CDS |
| TAGCCGAATATTAACTCATA+TGG | 0.334145 | 1.3:+79623215 | MS.gene046177:CDS |
| GAATTCAAGTCCTCTGTATA+CGG | 0.338048 | 1.3:+79622783 | MS.gene046177:CDS |
| GAGTTGAAATATTATCTTAC+AGG | 0.343089 | 1.3:+79623153 | MS.gene046177:CDS |
| TTTAAATTCACAATAGTTGA+TGG | 0.343775 | 1.3:+79611903 | MS.gene046177:CDS |
| TGGGGTGAGCTCGCTGATAC+TGG | 0.348714 | 1.3:+79622915 | MS.gene046177:CDS |
| GAAATACGTAAACACCATTT+AGG | 0.349059 | 1.3:-79610559 | None:intergenic |
| TCATAGAAAATATAATATTC+TGG | 0.351290 | 1.3:-79615852 | None:intergenic |
| GCTTCCTTCTTCCCAGAAAC+AGG | 0.372373 | 1.3:-79623186 | None:intergenic |
| TAACCTCGTCACTGGGATAA+AGG | 0.376712 | 1.3:-79615981 | None:intergenic |
| AAGAATTTACAAGACCTAAA+TGG | 0.378666 | 1.3:+79610545 | MS.gene046177:CDS |
| CGATTCCAACAAAGAATTTG+TGG | 0.378805 | 1.3:+79610254 | MS.gene046177:CDS |
| AGCTGATTTAATCAAGCGTT+TGG | 0.379332 | 1.3:+79617831 | MS.gene046177:CDS |
| GATACGTCAAACGTTTGATC+AGG | 0.384992 | 1.3:-79610929 | None:intergenic |
| AGATAAATCATGTGCATTCC+AGG | 0.390123 | 1.3:-79617601 | None:intergenic |
| GACACAATCTTCCTGTTTCT+GGG | 0.397267 | 1.3:+79623175 | MS.gene046177:CDS |
| CCTGCAGACCAGGAGTGGTC+AGG | 0.405236 | 1.3:+79622751 | MS.gene046177:intron |
| CTGGACAAGCCTTCCTCATC+AGG | 0.406253 | 1.3:-79622270 | None:intergenic |
| GTAAACCACAAATTCTTTGT+TGG | 0.415961 | 1.3:-79610259 | None:intergenic |
| ATATTGTACCTGCGCAATAA+GGG | 0.416801 | 1.3:-79617396 | None:intergenic |
| CTTTCAGGATTTGATTGGCT+TGG | 0.421972 | 1.3:+79611037 | MS.gene046177:intron |
| TTAGTTCGTCCAACTGCTCC+TGG | 0.426762 | 1.3:+79611954 | MS.gene046177:CDS |
| AGATACAGCCAAGGGCAATC+TGG | 0.428200 | 1.3:-79610350 | None:intergenic |
| TAATCTCTCAAGCAGCTTAG+TGG | 0.428314 | 1.3:-79615934 | None:intergenic |
| GTTAACTTTGTAGCCTCAGC+AGG | 0.436260 | 1.3:+79611074 | MS.gene046177:CDS |
| GAAGTGGTAACGAACATGTT+AGG | 0.438871 | 1.3:-79610297 | None:intergenic |
| CGTTTCTTGGAGGCTTCAGT+TGG | 0.440412 | 1.3:-79622846 | None:intergenic |
| GCACCTTCATTGCCCTTCAT+AGG | 0.447649 | 1.3:+79616626 | MS.gene046177:CDS |
| GAGACACTATGCAGTATTGC+AGG | 0.448682 | 1.3:+79622197 | MS.gene046177:CDS |
| TCTAATGCCCTGACCACTCC+TGG | 0.455133 | 1.3:-79622759 | None:intergenic |
| TAGGATGAGGTTATCCAATC+TGG | 0.457752 | 1.3:+79617562 | MS.gene046177:intron |
| GGTCCATGGTACAGCTCAAC+CGG | 0.459841 | 1.3:+79616647 | MS.gene046177:CDS |
| CAGGATTTGATTGGCTTGGA+AGG | 0.462987 | 1.3:+79611041 | MS.gene046177:intron |
| TCTTCCTGTTTCTGGGAAGA+AGG | 0.465621 | 1.3:+79623182 | MS.gene046177:CDS |
| GTATTTCATGTTGCTGAAGA+AGG | 0.467074 | 1.3:+79610575 | MS.gene046177:CDS |
| AGCCAGCATGGTGTCATGTC+CGG | 0.468568 | 1.3:+79610680 | MS.gene046177:CDS |
| GCATTTCAATTTCAGATTGG+AGG | 0.478258 | 1.3:+79611827 | MS.gene046177:intron |
| TAGTTCATCTCGACCTCAAT+TGG | 0.478790 | 1.3:+79617370 | MS.gene046177:CDS |
| GCTAAAGACACACAGGATCT+TGG | 0.483916 | 1.3:+79610875 | MS.gene046177:CDS |
| CTGGGATAAAGGAAAGTTGA+TGG | 0.489595 | 1.3:-79615970 | None:intergenic |
| GCTCAATCTGACCACCAGAT+TGG | 0.491292 | 1.3:-79617576 | None:intergenic |
| CCCTAAAAGATGTAGATGTC+CGG | 0.492151 | 1.3:-79615905 | None:intergenic |
| ACTTTCATTTGTGCTGATAC+CGG | 0.492393 | 1.3:+79613292 | MS.gene046177:CDS |
| CTTGTCATGTCGGATTTGAA+AGG | 0.494218 | 1.3:-79610836 | None:intergenic |
| GCAATAAGGGCGACCAATTG+AGG | 0.497738 | 1.3:-79617383 | None:intergenic |
| TCCTCTCTACAATGCTATCT+GGG | 0.498058 | 1.3:+79610715 | MS.gene046177:CDS |
| AAGAACAGTAACCTCGTCAC+TGG | 0.498647 | 1.3:-79615989 | None:intergenic |
| AATTCAAGTCCTCTGTATAC+GGG | 0.498771 | 1.3:+79622784 | MS.gene046177:CDS |
| ATAACATTCATCACCTTCAA+AGG | 0.501406 | 1.3:-79612498 | None:intergenic |
| TTAAATTCACAATAGTTGAT+GGG | 0.505751 | 1.3:+79611904 | MS.gene046177:CDS |
| AGTCTAATGTCATCAGAATA+CGG | 0.508989 | 1.3:-79617625 | None:intergenic |
| AGCCGAATATTAACTCATAT+GGG | 0.509528 | 1.3:+79623216 | MS.gene046177:CDS |
| TCTCACTTCCAGATTGCCCT+TGG | 0.513175 | 1.3:+79610342 | MS.gene046177:CDS |
| TACATACTTGTTTCCTGCTG+AGG | 0.514740 | 1.3:-79611087 | None:intergenic |
| CAGTGTTGTTCTTGTCATGT+CGG | 0.514990 | 1.3:-79610846 | None:intergenic |
| AGAGTGTGAGAACTATGATT+GGG | 0.515732 | 1.3:+79622896 | MS.gene046177:CDS |
| CATTGCCCTTCATAGGTCCA+TGG | 0.517743 | 1.3:+79616633 | MS.gene046177:CDS |
| GAGGAAAGTGAGCTTGGCAC+TGG | 0.519460 | 1.3:+79622819 | MS.gene046177:CDS |
| GGTGGTCAGATTGAGCCGCC+TGG | 0.519634 | 1.3:+79617583 | MS.gene046177:CDS |
| ATTGGTCGCCCTTATTGCGC+AGG | 0.520771 | 1.3:+79617388 | MS.gene046177:CDS |
| GGACCTATGAAGGGCAATGA+AGG | 0.522674 | 1.3:-79616629 | None:intergenic |
| GCTGTACCATGGACCTATGA+AGG | 0.524415 | 1.3:-79616639 | None:intergenic |
| TATGATGAGGAAAGTGAGCT+TGG | 0.525175 | 1.3:+79622813 | MS.gene046177:CDS |
| AGAAAGAATGGATGGTGAAG+AGG | 0.528556 | 1.3:+79610161 | None:intergenic |
| TTTATAAGGATGAAACCGTC+TGG | 0.539775 | 1.3:-79613335 | None:intergenic |
| CTGATGAGGAAGGCTTGTCC+AGG | 0.540037 | 1.3:+79622271 | MS.gene046177:CDS |
| CTGCAGACCAGGAGTGGTCA+GGG | 0.540525 | 1.3:+79622752 | MS.gene046177:intron |
| AGCGTTATTCAGATACTAGT+GGG | 0.545399 | 1.3:+79617770 | MS.gene046177:intron |
| AGGAAGAAGCTTACCAGGGT+TGG | 0.546299 | 1.3:-79617884 | None:intergenic |
| AAGAGTGTGAGAACTATGAT+TGG | 0.546419 | 1.3:+79622895 | MS.gene046177:CDS |
| TTCAGATTCAGAAGAAGAAG+TGG | 0.547042 | 1.3:-79610313 | None:intergenic |
| TTCCGGACATGACACCATGC+TGG | 0.548842 | 1.3:-79610682 | None:intergenic |
| GCTCAAGCACTTCTTCGTAA+AGG | 0.551563 | 1.3:+79610740 | MS.gene046177:CDS |
| TGTTGGTCTGTCAAGATAGT+CGG | 0.552870 | 1.3:-79610600 | None:intergenic |
| TGTTTGATAAGTTTAGCTGT+TGG | 0.553645 | 1.3:-79610617 | None:intergenic |
| AAATGCTTACCAGGAGCAGT+TGG | 0.556970 | 1.3:-79611963 | None:intergenic |
| AGTATTGCAGGCATTAGCTC+TGG | 0.560108 | 1.3:+79622209 | MS.gene046177:CDS |
| AGAATGGATGGTGAAGAGGA+AGG | 0.560673 | 1.3:+79610165 | None:intergenic |
| AGATGAAACATTACCTGATG+AGG | 0.570932 | 1.3:+79622257 | MS.gene046177:CDS |
| ATACGTCAAACGTTTGATCA+GGG | 0.574569 | 1.3:-79610928 | None:intergenic |
| GCAGGCATTAGCTCTGGAAG+AGG | 0.576363 | 1.3:+79622215 | MS.gene046177:CDS |
| AGATTCTTCTATCAATGCAC+CGG | 0.577013 | 1.3:-79613311 | None:intergenic |
| CAGCGTTATTCAGATACTAG+TGG | 0.592622 | 1.3:+79617769 | MS.gene046177:intron |
| AGGGTTTGAGAAAGAATGGA+TGG | 0.598793 | 1.3:+79610153 | None:intergenic |
| TGACATTAGACTTGTTGAAG+AGG | 0.603928 | 1.3:+79617636 | MS.gene046177:CDS |
| GTTGAAGGATTTGACAGTGG+TGG | 0.605755 | 1.3:+79623131 | MS.gene046177:intron |
| GGTCCTTTGAGAGAGCCAAA+AGG | 0.607281 | 1.3:-79610815 | None:intergenic |
| TGAGCTCGCTGATACTGGAA+AGG | 0.617414 | 1.3:+79622920 | MS.gene046177:CDS |
| ACGTTTGATCAGGGAAACTG+AGG | 0.618953 | 1.3:-79610919 | None:intergenic |
| GACGATGAAGACGATCCTGA+TGG | 0.623897 | 1.3:+79610207 | MS.gene046177:CDS |
| GAAATTAAGAGAGTTGCTAC+CGG | 0.624896 | 1.3:+79615886 | MS.gene046177:CDS |
| CGAGTCAGAGATACAGCCAA+GGG | 0.628004 | 1.3:-79610358 | None:intergenic |
| GCGAGTCAGAGATACAGCCA+AGG | 0.630923 | 1.3:-79610359 | None:intergenic |
| GATGTGTAGGAGCAATCACA+TGG | 0.632751 | 1.3:+79612454 | MS.gene046177:intron |
| CGTTTGATCAGGGAAACTGA+GGG | 0.635741 | 1.3:-79610918 | None:intergenic |
| CTGTACCATGGACCTATGAA+GGG | 0.637827 | 1.3:-79616638 | None:intergenic |
| GAAACATTACCTGATGAGGA+AGG | 0.638915 | 1.3:+79622261 | MS.gene046177:CDS |
| GAGTGTGAGAACTATGATTG+GGG | 0.639036 | 1.3:+79622897 | MS.gene046177:CDS |
| ATACCGGTTGAGCTGTACCA+TGG | 0.640613 | 1.3:-79616650 | None:intergenic |
| TACTGCATAGTGTCTCTGCA+AGG | 0.644229 | 1.3:-79622190 | None:intergenic |
| AATCACCTGCAGACCAGGAG+TGG | 0.644588 | 1.3:+79622746 | MS.gene046177:intron |
| AGAACAGTAACCTCGTCACT+GGG | 0.652217 | 1.3:-79615988 | None:intergenic |
| ACCCAGATAGCATTGTAGAG+AGG | 0.656227 | 1.3:-79610716 | None:intergenic |
| TTTCCTTTATCCCAGTGACG+AGG | 0.659770 | 1.3:+79615978 | MS.gene046177:CDS |
| GTATTCCAAATCACCAACCC+TGG | 0.666292 | 1.3:+79617871 | MS.gene046177:CDS |
| TTATTCAGATACTAGTGGGG+TGG | 0.669544 | 1.3:+79617774 | MS.gene046177:CDS |
| TGTTAGGAGATGCATCGACA+AGG | 0.676094 | 1.3:-79610281 | None:intergenic |
| TGATAGAAGAATCTACCAGA+CGG | 0.676569 | 1.3:+79613320 | MS.gene046177:CDS |
| GATGAGGTTATCCAATCTGG+TGG | 0.676798 | 1.3:+79617565 | MS.gene046177:CDS |
| ATAATTCTCCCCGTATACAG+AGG | 0.677348 | 1.3:-79622793 | None:intergenic |
| GCAGAGAGCTAAAGACACAC+AGG | 0.680327 | 1.3:+79610868 | MS.gene046177:CDS |
| GCAGTTGAAGGATTTGACAG+TGG | 0.693560 | 1.3:+79623128 | MS.gene046177:intron |
| ATACGGGGAGAATTATGATG+AGG | 0.697455 | 1.3:+79622800 | MS.gene046177:CDS |
| ATTCAAGTCCTCTGTATACG+GGG | 0.705602 | 1.3:+79622785 | MS.gene046177:CDS |
| TAAATCATGTGCATTCCAGG+CGG | 0.726525 | 1.3:-79617598 | None:intergenic |
| GCGTTATTCAGATACTAGTG+GGG | 0.735980 | 1.3:+79617771 | MS.gene046177:intron |
| GTTTGATCAGGGAAACTGAG+GGG | 0.767489 | 1.3:-79610917 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 79610168 | 79623245 | 79610168 | ID=MS.gene046177 |
| chr1.3 | mRNA | 79610168 | 79623245 | 79610168 | ID=MS.gene046177.t1;Parent=MS.gene046177 |
| chr1.3 | exon | 79610168 | 79610440 | 79610168 | ID=MS.gene046177.t1.exon1;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79610168 | 79610440 | 79610168 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79610536 | 79610964 | 79610536 | ID=MS.gene046177.t1.exon2;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79610536 | 79610964 | 79610536 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79611044 | 79611102 | 79611044 | ID=MS.gene046177.t1.exon3;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79611044 | 79611102 | 79611044 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79611842 | 79611975 | 79611842 | ID=MS.gene046177.t1.exon4;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79611842 | 79611975 | 79611842 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79612463 | 79612506 | 79612463 | ID=MS.gene046177.t1.exon5;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79612463 | 79612506 | 79612463 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79613283 | 79613359 | 79613283 | ID=MS.gene046177.t1.exon6;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79613283 | 79613359 | 79613283 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79615852 | 79615999 | 79615852 | ID=MS.gene046177.t1.exon7;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79615852 | 79615999 | 79615852 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79616607 | 79616668 | 79616607 | ID=MS.gene046177.t1.exon8;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79616607 | 79616668 | 79616607 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79617349 | 79617409 | 79617349 | ID=MS.gene046177.t1.exon9;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79617349 | 79617409 | 79617349 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79617565 | 79617657 | 79617565 | ID=MS.gene046177.t1.exon10;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79617565 | 79617657 | 79617565 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79617772 | 79617892 | 79617772 | ID=MS.gene046177.t1.exon11;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79617772 | 79617892 | 79617772 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79622190 | 79622292 | 79622190 | ID=MS.gene046177.t1.exon12;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79622190 | 79622292 | 79622190 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79622758 | 79622941 | 79622758 | ID=MS.gene046177.t1.exon13;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79622758 | 79622941 | 79622758 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| chr1.3 | exon | 79623132 | 79623245 | 79623132 | ID=MS.gene046177.t1.exon14;Parent=MS.gene046177.t1 |
| chr1.3 | CDS | 79623132 | 79623245 | 79623132 | ID=cds.MS.gene046177.t1;Parent=MS.gene046177.t1 |
| Gene Sequence |
| Protein sequence |
