Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08691.t1 | XP_003606367.1 | 99 | 203 | 2 | 0 | 99 | 301 | 7 | 209 | 2.90E-108 | 401.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08691.t1 | Q8RUN2 | 90.8 | 196 | 18 | 0 | 101 | 296 | 4 | 199 | 1.4e-98 | 360.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08691.t1 | G7JND4 | 99.0 | 203 | 2 | 0 | 99 | 301 | 7 | 209 | 2.1e-108 | 401.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene08691 | 0.827672 | 1.37E-54 | -1.69E-46 |
| MS.gene049151 | MS.gene08691 | 0.82418 | 9.28E-54 | -1.69E-46 |
| MS.gene049153 | MS.gene08691 | 0.821791 | 3.35E-53 | -1.69E-46 |
| MS.gene049260 | MS.gene08691 | 0.858607 | 7.33E-63 | -1.69E-46 |
| MS.gene049356 | MS.gene08691 | 0.820649 | 6.14E-53 | -1.69E-46 |
| MS.gene049389 | MS.gene08691 | 0.811355 | 7.30E-51 | -1.69E-46 |
| MS.gene049491 | MS.gene08691 | 0.802038 | 6.78E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene08691 | 0.874695 | 5.52E-68 | -1.69E-46 |
| MS.gene050128 | MS.gene08691 | 0.994189 | 2.91E-205 | -1.69E-46 |
| MS.gene050301 | MS.gene08691 | 0.872539 | 2.94E-67 | -1.69E-46 |
| MS.gene050529 | MS.gene08691 | 0.800635 | 1.31E-48 | -1.69E-46 |
| MS.gene050685 | MS.gene08691 | 0.844815 | 5.98E-59 | -1.69E-46 |
| MS.gene050762 | MS.gene08691 | 0.820952 | 5.23E-53 | -1.69E-46 |
| MS.gene050763 | MS.gene08691 | 0.821088 | 4.86E-53 | -1.69E-46 |
| MS.gene050971 | MS.gene08691 | 0.805973 | 1.03E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene08691 | 0.818737 | 1.68E-52 | -1.69E-46 |
| MS.gene051663 | MS.gene08691 | 0.805325 | 1.41E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene08691 | 0.840036 | 1.11E-57 | -1.69E-46 |
| MS.gene052078 | MS.gene08691 | 0.820356 | 7.16E-53 | -1.69E-46 |
| MS.gene052178 | MS.gene08691 | 0.895973 | 5.76E-76 | -1.69E-46 |
| MS.gene052188 | MS.gene08691 | 0.858478 | 8.01E-63 | -1.69E-46 |
| MS.gene052326 | MS.gene08691 | 0.804303 | 2.31E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene08691 | 0.84889 | 4.59E-60 | -1.69E-46 |
| MS.gene053073 | MS.gene08691 | 0.848807 | 4.84E-60 | -1.69E-46 |
| MS.gene05316 | MS.gene08691 | 0.903419 | 3.54E-79 | -1.69E-46 |
| MS.gene053204 | MS.gene08691 | 0.803 | 4.30E-49 | -1.69E-46 |
| MS.gene053209 | MS.gene08691 | 0.807495 | 4.92E-50 | -1.69E-46 |
| MS.gene053555 | MS.gene08691 | 0.809482 | 1.85E-50 | -1.69E-46 |
| MS.gene053557 | MS.gene08691 | 0.870692 | 1.20E-66 | -1.69E-46 |
| MS.gene053559 | MS.gene08691 | 0.848303 | 6.68E-60 | -1.69E-46 |
| MS.gene053836 | MS.gene08691 | 0.849869 | 2.45E-60 | -1.69E-46 |
| MS.gene053928 | MS.gene08691 | 0.815414 | 9.36E-52 | -1.69E-46 |
| MS.gene054010 | MS.gene08691 | 0.833886 | 4.12E-56 | -1.69E-46 |
| MS.gene054011 | MS.gene08691 | 0.846062 | 2.75E-59 | -1.69E-46 |
| MS.gene054012 | MS.gene08691 | 0.838087 | 3.54E-57 | -1.69E-46 |
| MS.gene054013 | MS.gene08691 | 0.836708 | 7.99E-57 | -1.69E-46 |
| MS.gene054123 | MS.gene08691 | 0.856429 | 3.24E-62 | -1.69E-46 |
| MS.gene054214 | MS.gene08691 | 0.850895 | 1.26E-60 | -1.69E-46 |
| MS.gene054349 | MS.gene08691 | 0.817249 | 3.64E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene08691 | 0.812348 | 4.44E-51 | -1.69E-46 |
| MS.gene055133 | MS.gene08691 | 0.823099 | 1.66E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene08691 | 0.841081 | 5.90E-58 | -1.69E-46 |
| MS.gene055390 | MS.gene08691 | 0.83157 | 1.55E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene08691 | 0.856097 | 4.05E-62 | -1.69E-46 |
| MS.gene055428 | MS.gene08691 | 0.802673 | 5.02E-49 | -1.69E-46 |
| MS.gene055604 | MS.gene08691 | 0.87018 | 1.77E-66 | -1.69E-46 |
| MS.gene056410 | MS.gene08691 | 0.806178 | 9.33E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene08691 | 0.820305 | 7.36E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene08691 | 0.830172 | 3.41E-55 | -1.69E-46 |
| MS.gene056659 | MS.gene08691 | 0.808607 | 2.85E-50 | -1.69E-46 |
| MS.gene056772 | MS.gene08691 | 0.806772 | 6.99E-50 | -1.69E-46 |
| MS.gene056902 | MS.gene08691 | 0.843763 | 1.15E-58 | -1.69E-46 |
| MS.gene056931 | MS.gene08691 | 0.80188 | 7.31E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene08691 | 0.805389 | 1.37E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene08691 | 0.814852 | 1.25E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene08691 | 0.862639 | 4.40E-64 | -1.69E-46 |
| MS.gene057449 | MS.gene08691 | 0.861915 | 7.33E-64 | -1.69E-46 |
| MS.gene057450 | MS.gene08691 | 0.866779 | 2.22E-65 | -1.69E-46 |
| MS.gene057537 | MS.gene08691 | 0.867856 | 1.00E-65 | -1.69E-46 |
| MS.gene057752 | MS.gene08691 | 0.819852 | 9.34E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene08691 | 0.810669 | 1.03E-50 | -1.69E-46 |
| MS.gene057794 | MS.gene08691 | 0.830366 | 3.06E-55 | -1.69E-46 |
| MS.gene057796 | MS.gene08691 | 0.833172 | 6.21E-56 | -1.69E-46 |
| MS.gene057927 | MS.gene08691 | 0.803011 | 4.27E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene08691 | 0.8409 | 6.58E-58 | -1.69E-46 |
| MS.gene058082 | MS.gene08691 | 0.825327 | 4.98E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene08691 | 0.84132 | 5.10E-58 | -1.69E-46 |
| MS.gene058142 | MS.gene08691 | 0.810671 | 1.03E-50 | -1.69E-46 |
| MS.gene058365 | MS.gene08691 | 0.822083 | 2.86E-53 | -1.69E-46 |
| MS.gene058433 | MS.gene08691 | 0.806361 | 8.54E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene08691 | 0.821265 | 4.43E-53 | -1.69E-46 |
| MS.gene058845 | MS.gene08691 | 0.816563 | 5.19E-52 | -1.69E-46 |
| MS.gene058928 | MS.gene08691 | 0.83986 | 1.23E-57 | -1.69E-46 |
| MS.gene058949 | MS.gene08691 | 0.819681 | 1.02E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene08691 | 0.842697 | 2.21E-58 | -1.69E-46 |
| MS.gene059228 | MS.gene08691 | 0.833284 | 5.82E-56 | -1.69E-46 |
| MS.gene059294 | MS.gene08691 | 0.863171 | 3.01E-64 | -1.69E-46 |
| MS.gene059366 | MS.gene08691 | 0.813788 | 2.14E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene08691 | 0.822057 | 2.90E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene08691 | 0.839594 | 1.44E-57 | -1.69E-46 |
| MS.gene05959 | MS.gene08691 | 0.819647 | 1.04E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene08691 | 0.811652 | 6.29E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene08691 | 0.811263 | 7.64E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene08691 | 0.829671 | 4.52E-55 | -1.69E-46 |
| MS.gene059695 | MS.gene08691 | 0.81973 | 9.96E-53 | -1.69E-46 |
| MS.gene059708 | MS.gene08691 | 0.84012 | 1.05E-57 | -1.69E-46 |
| MS.gene059807 | MS.gene08691 | 0.832438 | 9.45E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene08691 | 0.850509 | 1.62E-60 | -1.69E-46 |
| MS.gene059894 | MS.gene08691 | 0.867135 | 1.71E-65 | -1.69E-46 |
| MS.gene059899 | MS.gene08691 | 0.830009 | 3.74E-55 | -1.69E-46 |
| MS.gene059974 | MS.gene08691 | 0.844827 | 5.94E-59 | -1.69E-46 |
| MS.gene060005 | MS.gene08691 | 0.865007 | 8.06E-65 | -1.69E-46 |
| MS.gene06037 | MS.gene08691 | 0.848615 | 5.47E-60 | -1.69E-46 |
| MS.gene060495 | MS.gene08691 | 0.8911 | 5.40E-74 | -1.69E-46 |
| MS.gene060496 | MS.gene08691 | 0.823877 | 1.09E-53 | -1.69E-46 |
| MS.gene060799 | MS.gene08691 | 0.800856 | 1.18E-48 | -1.69E-46 |
| MS.gene060800 | MS.gene08691 | 0.852028 | 6.04E-61 | -1.69E-46 |
| MS.gene060801 | MS.gene08691 | 0.801636 | 8.20E-49 | -1.69E-46 |
| MS.gene060943 | MS.gene08691 | 0.81333 | 2.70E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene08691 | 0.865409 | 6.03E-65 | -1.69E-46 |
| MS.gene061051 | MS.gene08691 | -0.852444 | 4.60E-61 | -1.69E-46 |
| MS.gene061112 | MS.gene08691 | 0.824973 | 6.04E-54 | -1.69E-46 |
| MS.gene061265 | MS.gene08691 | 0.827535 | 1.48E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene034799 | MS.gene08691 | PPI |
| MS.gene08691 | MS.gene016181 | PPI |
| MS.gene08691 | MS.gene41079 | PPI |
| MS.gene08691 | MS.gene016180 | PPI |
| MS.gene33028 | MS.gene08691 | PPI |
| MS.gene08691 | MS.gene007521 | PPI |
| MS.gene08691 | MS.gene007426 | PPI |
| MS.gene08691 | MS.gene52386 | PPI |
| MS.gene24610 | MS.gene08691 | PPI |
| MS.gene08691 | MS.gene20068 | PPI |
| MS.gene051577 | MS.gene08691 | PPI |
| MS.gene08691 | MS.gene055648 | PPI |
| MS.gene22428 | MS.gene08691 | PPI |
| MS.gene08691 | MS.gene029824 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08691.t1 | MTR_4g058740 | 99.043 | 209 | 2 | 0 | 93 | 301 | 1 | 209 | 3.05e-152 | 424 |
| MS.gene08691.t1 | MTR_2g094790 | 88.517 | 209 | 24 | 0 | 93 | 301 | 1 | 209 | 1.29e-136 | 385 |
| MS.gene08691.t1 | MTR_1g064260 | 87.255 | 204 | 26 | 0 | 93 | 296 | 1 | 204 | 4.47e-131 | 371 |
| MS.gene08691.t1 | MTR_7g101290 | 87.940 | 199 | 24 | 0 | 98 | 296 | 7 | 205 | 6.90e-129 | 366 |
| MS.gene08691.t1 | MTR_0041s0100 | 87.940 | 199 | 24 | 0 | 98 | 296 | 7 | 205 | 6.90e-129 | 366 |
| MS.gene08691.t1 | MTR_3g113710 | 72.350 | 217 | 53 | 1 | 92 | 301 | 2 | 218 | 1.06e-114 | 330 |
| MS.gene08691.t1 | MTR_1g057020 | 75.377 | 199 | 49 | 0 | 98 | 296 | 2 | 200 | 5.90e-111 | 320 |
| MS.gene08691.t1 | MTR_3g113710 | 75.661 | 189 | 46 | 0 | 92 | 280 | 2 | 190 | 2.06e-106 | 310 |
| MS.gene08691.t1 | MTR_1g015830 | 68.269 | 208 | 62 | 1 | 93 | 300 | 1 | 204 | 3.73e-103 | 300 |
| MS.gene08691.t1 | MTR_1g064260 | 88.514 | 148 | 17 | 0 | 149 | 296 | 1 | 148 | 1.15e-93 | 274 |
| MS.gene08691.t1 | MTR_1g015830 | 69.079 | 152 | 47 | 0 | 149 | 300 | 1 | 152 | 1.12e-74 | 226 |
| MS.gene08691.t1 | MTR_3g113710 | 74.126 | 143 | 30 | 1 | 166 | 301 | 10 | 152 | 3.84e-72 | 219 |
| MS.gene08691.t1 | MTR_1g024145 | 71.845 | 103 | 29 | 0 | 120 | 222 | 150 | 252 | 1.06e-46 | 158 |
| MS.gene08691.t1 | MTR_1g024850 | 72.857 | 70 | 19 | 0 | 102 | 171 | 14 | 83 | 7.47e-32 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08691.t1 | AT2G28305 | 90.816 | 196 | 18 | 0 | 101 | 296 | 4 | 199 | 2.29e-131 | 372 |
| MS.gene08691.t1 | AT2G37210 | 84.211 | 209 | 32 | 1 | 92 | 300 | 2 | 209 | 1.09e-128 | 365 |
| MS.gene08691.t1 | AT3G53450 | 82.857 | 210 | 35 | 1 | 93 | 301 | 1 | 210 | 1.31e-126 | 360 |
| MS.gene08691.t1 | AT2G37210 | 76.106 | 226 | 30 | 1 | 99 | 300 | 8 | 233 | 1.21e-122 | 351 |
| MS.gene08691.t1 | AT2G28305 | 92.778 | 180 | 13 | 0 | 101 | 280 | 4 | 183 | 8.95e-122 | 347 |
| MS.gene08691.t1 | AT5G06300 | 78.894 | 199 | 42 | 0 | 98 | 296 | 2 | 200 | 6.41e-116 | 333 |
| MS.gene08691.t1 | AT5G03270 | 79.793 | 193 | 39 | 0 | 95 | 287 | 9 | 201 | 1.49e-111 | 321 |
| MS.gene08691.t1 | AT5G03270 | 79.787 | 188 | 38 | 0 | 95 | 282 | 9 | 196 | 1.57e-107 | 311 |
| MS.gene08691.t1 | AT2G35990 | 69.849 | 199 | 60 | 0 | 98 | 296 | 2 | 200 | 1.91e-107 | 311 |
| MS.gene08691.t1 | AT4G35190 | 71.782 | 202 | 57 | 0 | 98 | 299 | 2 | 203 | 1.37e-106 | 310 |
| MS.gene08691.t1 | AT5G11950 | 67.164 | 201 | 66 | 0 | 100 | 300 | 5 | 205 | 2.18e-101 | 296 |
| MS.gene08691.t1 | AT5G11950 | 67.164 | 201 | 66 | 0 | 100 | 300 | 5 | 205 | 2.18e-101 | 296 |
| MS.gene08691.t1 | AT5G11950 | 67.164 | 201 | 66 | 0 | 100 | 300 | 5 | 205 | 2.18e-101 | 296 |
| MS.gene08691.t1 | AT4G35190 | 73.545 | 189 | 50 | 0 | 98 | 286 | 2 | 190 | 2.98e-101 | 295 |
| MS.gene08691.t1 | AT2G28305 | 92.647 | 136 | 10 | 0 | 101 | 236 | 4 | 139 | 2.02e-88 | 261 |
| MS.gene08691.t1 | AT2G35990 | 70.270 | 148 | 44 | 0 | 149 | 296 | 1 | 148 | 2.74e-77 | 233 |
| MS.gene08691.t1 | AT2G35990 | 70.270 | 148 | 44 | 0 | 149 | 296 | 1 | 148 | 2.74e-77 | 233 |
| MS.gene08691.t1 | AT5G11950 | 67.105 | 152 | 50 | 0 | 149 | 300 | 1 | 152 | 2.06e-73 | 223 |
| MS.gene08691.t1 | AT5G26140 | 55.102 | 147 | 44 | 1 | 176 | 300 | 6 | 152 | 4.83e-47 | 155 |
| MS.gene08691.t1 | AT5G26140 | 54.135 | 133 | 39 | 1 | 190 | 300 | 1 | 133 | 1.47e-40 | 138 |
| MS.gene08691.t1 | AT5G26140 | 58.824 | 102 | 42 | 0 | 199 | 300 | 17 | 118 | 2.94e-36 | 127 |
Find 75 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAATATTGATTTGGTTTA+TGG | 0.095929 | 4.4:-44973300 | MS.gene08691:CDS |
| CGTTGTCATGGTTGTGTTTC+CGG | 0.116343 | 4.4:-44976489 | MS.gene08691:CDS |
| ACAATTATATGACGAGCTTT+AGG | 0.212084 | 4.4:+44967772 | None:intergenic |
| TTTCAAGTACCTCCATCTTT+TGG | 0.280036 | 4.4:+44967722 | None:intergenic |
| CAATTATATGACGAGCTTTA+GGG | 0.317659 | 4.4:+44967773 | None:intergenic |
| CCGGAAAAGAGTGTTCTTAT+AGG | 0.325458 | 4.4:-44976629 | MS.gene08691:intron |
| TCTAAGCAAAGTTGGGAAAC+TGG | 0.328148 | 4.4:-44967128 | MS.gene08691:CDS |
| TGAAAGAATCAAAGTTTAAG+AGG | 0.352776 | 4.4:-44974734 | MS.gene08691:CDS |
| GGTGTCAAGAAATATTGATT+TGG | 0.365193 | 4.4:-44973307 | MS.gene08691:CDS |
| AGGAGGCAGCATTGGTTTGA+TGG | 0.380464 | 4.4:-44973277 | MS.gene08691:CDS |
| CAGCATTGGTTTGATGGGGT+TGG | 0.384600 | 4.4:-44973271 | MS.gene08691:CDS |
| GGAGGCAGCATTGGTTTGAT+GGG | 0.391809 | 4.4:-44973276 | MS.gene08691:CDS |
| GATGATGATGCCTGCAACAT+TGG | 0.409310 | 4.4:-44976673 | MS.gene08691:CDS |
| GTTTCTCAAGCTGTTTATGA+TGG | 0.421989 | 4.4:-44973249 | MS.gene08691:CDS |
| TTCGGCGAGGCCAATGTTGC+AGG | 0.423088 | 4.4:+44976663 | None:intergenic |
| CATCATAAAATTACCTCTCT+TGG | 0.431675 | 4.4:+44973080 | None:intergenic |
| ATCTCTGTCGTCGTTAAGCT+CGG | 0.432455 | 4.4:+44976693 | None:intergenic |
| TCTCTTGGCATCAGTGTCCT+TGG | 0.437369 | 4.4:+44973095 | None:intergenic |
| TATATGCATGAAGGTGGCTA+TGG | 0.441783 | 4.4:-44968035 | MS.gene08691:intron |
| GACGGCAACGTTGGCCTCTC+CGG | 0.452022 | 4.4:+44976470 | None:intergenic |
| GTTTATGGAGGAGGCAGCAT+TGG | 0.458836 | 4.4:-44973285 | MS.gene08691:CDS |
| GGAAACTTACTGGTTTATCA+TGG | 0.459986 | 4.4:+44967964 | None:intergenic |
| GATGCAACTCTTTCATGTTG+TGG | 0.462188 | 4.4:+44967149 | None:intergenic |
| AAAGATGCTGCTATTGAGCT+TGG | 0.470913 | 4.4:-44974666 | MS.gene08691:CDS |
| CGTTGCCGTCGTCGTTGTCA+TGG | 0.473578 | 4.4:-44976457 | MS.gene08691:CDS |
| CTAAGCAAAGTTGGGAAACT+GGG | 0.478869 | 4.4:-44967127 | MS.gene08691:CDS |
| GTGGGATTGTTGAATGTAGA+TGG | 0.481596 | 4.4:-44967853 | MS.gene08691:CDS |
| AGCTTCTTGAGGTCATAACT+TGG | 0.481664 | 4.4:-44968001 | MS.gene08691:CDS |
| GATGGAGGTCGACATGTGAT+TGG | 0.485208 | 4.4:-44973231 | MS.gene08691:CDS |
| ATTGATTTGGTTTATGGAGG+AGG | 0.486097 | 4.4:-44973294 | MS.gene08691:CDS |
| ACATTGGCCTCGCCGAACAT+TGG | 0.491173 | 4.4:-44976657 | MS.gene08691:CDS |
| TTTCCGGCGAGGCCAATGTT+CGG | 0.492246 | 4.4:+44976645 | None:intergenic |
| GTTGGCGACAGCGGCAAAGT+TGG | 0.492921 | 4.4:+44976514 | None:intergenic |
| AAGTTGGGAAACTGGGAAGC+TGG | 0.495777 | 4.4:-44967120 | MS.gene08691:CDS |
| AGTTGCATCTAAGCAAAGTT+GGG | 0.497061 | 4.4:-44967135 | MS.gene08691:CDS |
| AATAAAAGCATCAGAATGCT+TGG | 0.500098 | 4.4:+44971289 | None:intergenic |
| GAATGGGAACCAACCTGGTA+AGG | 0.500409 | 4.4:+44971265 | None:intergenic |
| GGAGAAGTGAAGGCAGTAGC+AGG | 0.500561 | 4.4:-44971338 | MS.gene08691:CDS |
| AGTGGGTTTGTGCAGATCAC+TGG | 0.500630 | 4.4:-44971371 | MS.gene08691:intron |
| GTCATAACTTGGGCTCAACT+TGG | 0.508031 | 4.4:-44967990 | MS.gene08691:CDS |
| AATATTGATTTGGTTTATGG+AGG | 0.510397 | 4.4:-44973297 | MS.gene08691:CDS |
| TGTCGCCAACGTCGTTGTCA+TGG | 0.513053 | 4.4:-44976501 | MS.gene08691:CDS |
| TCGCCGAACATTGGCCTCGC+CGG | 0.513147 | 4.4:-44976648 | MS.gene08691:CDS |
| AAAAGAGCTTGTCCAAAAGA+TGG | 0.518338 | 4.4:-44967734 | MS.gene08691:CDS |
| AAGAAATAAAGAGATGGAGG+AGG | 0.519029 | 4.4:-44974766 | MS.gene08691:CDS |
| TAGAAGAAATAAAGAGATGG+AGG | 0.519415 | 4.4:-44974769 | MS.gene08691:CDS |
| GAGTTGCATCTAAGCAAAGT+TGG | 0.520245 | 4.4:-44967136 | MS.gene08691:CDS |
| GACAACGACGACGGCAACGT+TGG | 0.524614 | 4.4:+44976461 | None:intergenic |
| GAGGCAGCATTGGTTTGATG+GGG | 0.537940 | 4.4:-44973275 | MS.gene08691:CDS |
| AGTCGTTGACGTCGCTGTCA+TGG | 0.537982 | 4.4:-44976404 | MS.gene08691:CDS |
| ATTGACAAAGCTGTGGAGGA+AGG | 0.543403 | 4.4:-44967805 | MS.gene08691:CDS |
| AGAGACAGTGGGAGAAGTGA+AGG | 0.543939 | 4.4:-44971348 | MS.gene08691:CDS |
| TCTCAAGCTGTTTATGATGG+AGG | 0.551173 | 4.4:-44973246 | MS.gene08691:CDS |
| GTCAACGACTGCGGCAACGT+TGG | 0.554511 | 4.4:+44976417 | None:intergenic |
| TTATAGCTACTCTTGTTACC+AGG | 0.556974 | 4.4:+44974687 | None:intergenic |
| GCATCAAAGAAAAGCTGAGA+TGG | 0.557994 | 4.4:-44971312 | MS.gene08691:CDS |
| GACACTTGAGGAGCTTCTTG+AGG | 0.558323 | 4.4:-44968012 | MS.gene08691:CDS |
| GACAACGACGTTGGCGACAG+CGG | 0.563321 | 4.4:+44976505 | None:intergenic |
| ATGGAGGTCGACATGTGATT+GGG | 0.564949 | 4.4:-44973230 | MS.gene08691:intron |
| GAATAGAAGAAATAAAGAGA+TGG | 0.565618 | 4.4:-44974772 | MS.gene08691:CDS |
| ATCTTTCATTGACAAAGCTG+TGG | 0.565707 | 4.4:-44967812 | MS.gene08691:CDS |
| CACAACCATGACAACGACGT+TGG | 0.573420 | 4.4:+44976496 | None:intergenic |
| CACAACCATGACAACGACGA+CGG | 0.575758 | 4.4:+44976452 | None:intergenic |
| GCTTCTTGAGGTCATAACTT+GGG | 0.575878 | 4.4:-44968000 | MS.gene08691:CDS |
| ATATGCATGAAGGTGGCTAT+GGG | 0.577892 | 4.4:-44968034 | MS.gene08691:intron |
| TATTGAGCTTGGAAATGAGT+TGG | 0.583903 | 4.4:-44974655 | MS.gene08691:intron |
| TTTCATTGACAAAGCTGTGG+AGG | 0.587801 | 4.4:-44967809 | MS.gene08691:CDS |
| TCATGGTTGTGTTTCCGGAG+AGG | 0.592731 | 4.4:-44976484 | MS.gene08691:CDS |
| CAGATCACTGGAGAGACAGT+GGG | 0.608222 | 4.4:-44971359 | MS.gene08691:CDS |
| GCAGATCACTGGAGAGACAG+TGG | 0.632705 | 4.4:-44971360 | MS.gene08691:intron |
| GACAGCGACGTCAACGACTG+CGG | 0.642906 | 4.4:+44976408 | None:intergenic |
| AAGGACACTGATGCCAAGAG+AGG | 0.647568 | 4.4:-44973093 | MS.gene08691:intron |
| AGGTGGCTATGGGACACTTG+AGG | 0.653214 | 4.4:-44968024 | MS.gene08691:CDS |
| GGCGAGGCCAATGTTCGGCG+AGG | 0.660571 | 4.4:+44976650 | None:intergenic |
| AGAGCTTGTCCAAAAGATGG+AGG | 0.667572 | 4.4:-44967731 | MS.gene08691:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 44967115 | 44976733 | 44967115 | ID=MS.gene08691 |
| chr4.4 | mRNA | 44967115 | 44976733 | 44967115 | ID=MS.gene08691.t1;Parent=MS.gene08691 |
| chr4.4 | exon | 44976630 | 44976733 | 44976630 | ID=MS.gene08691.t1.exon1;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44976630 | 44976733 | 44976630 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44976400 | 44976537 | 44976400 | ID=MS.gene08691.t1.exon2;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44976400 | 44976537 | 44976400 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44974656 | 44974809 | 44974656 | ID=MS.gene08691.t1.exon3;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44974656 | 44974809 | 44974656 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44973231 | 44973328 | 44973231 | ID=MS.gene08691.t1.exon4;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44973231 | 44973328 | 44973231 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44973094 | 44973124 | 44973094 | ID=MS.gene08691.t1.exon5;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44973094 | 44973124 | 44973094 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44971279 | 44971378 | 44971279 | ID=MS.gene08691.t1.exon6;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44971279 | 44971378 | 44971279 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44967974 | 44968044 | 44967974 | ID=MS.gene08691.t1.exon7;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44967974 | 44968044 | 44967974 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44967732 | 44967875 | 44967732 | ID=MS.gene08691.t1.exon8;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44967732 | 44967875 | 44967732 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| chr4.4 | exon | 44967115 | 44967180 | 44967115 | ID=MS.gene08691.t1.exon9;Parent=MS.gene08691.t1 |
| chr4.4 | CDS | 44967115 | 44967180 | 44967115 | ID=cds.MS.gene08691.t1;Parent=MS.gene08691.t1 |
| Gene Sequence |
| Protein sequence |
