AlfalfaGEDB | MS.gene82546

M. sativa cultivar XinJiangDaYe / MS.gene82546

KEGG: K11254
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF15511.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82546.t1	KCW52694.1	100	103	0	0	1	103	4	106	7.50E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82546.t1	P62785	100.0	103	0	0	1	103	1	103	1.8e-50	199.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82546.t1	M5VRN0	100.0	103	0	0	1	103	1	103	5.4e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene82546	0.806315	8.73E-50	-1.69E-46
MS.gene049328	MS.gene82546	0.80294	4.42E-49	-1.69E-46
MS.gene049821	MS.gene82546	0.88645	3.38E-72	-1.69E-46
MS.gene049850	MS.gene82546	0.803597	3.23E-49	-1.69E-46
MS.gene050310	MS.gene82546	0.826167	3.15E-54	-1.69E-46
MS.gene050648	MS.gene82546	0.809226	2.10E-50	-1.69E-46
MS.gene050689	MS.gene82546	0.80141	9.12E-49	-1.69E-46
MS.gene050838	MS.gene82546	0.803445	3.47E-49	-1.69E-46
MS.gene050901	MS.gene82546	0.880067	7.44E-70	-1.69E-46
MS.gene05092	MS.gene82546	0.814109	1.82E-51	-1.69E-46
MS.gene051263	MS.gene82546	0.818285	2.12E-52	-1.69E-46
MS.gene051279	MS.gene82546	0.800006	1.76E-48	-1.69E-46
MS.gene05157	MS.gene82546	0.843832	1.10E-58	-1.69E-46
MS.gene051664	MS.gene82546	0.83778	4.25E-57	-1.69E-46
MS.gene051719	MS.gene82546	0.831923	1.27E-55	-1.69E-46
MS.gene051720	MS.gene82546	0.851353	9.38E-61	-1.69E-46
MS.gene052133	MS.gene82546	0.859269	4.65E-63	-1.69E-46
MS.gene053122	MS.gene82546	0.844375	7.86E-59	-1.69E-46
MS.gene053144	MS.gene82546	0.809369	1.96E-50	-1.69E-46
MS.gene053171	MS.gene82546	0.80151	8.71E-49	-1.69E-46
MS.gene053226	MS.gene82546	0.822598	2.17E-53	-1.69E-46
MS.gene053541	MS.gene82546	0.821268	4.42E-53	-1.69E-46
MS.gene053542	MS.gene82546	0.827916	1.20E-54	-1.69E-46
MS.gene053581	MS.gene82546	0.845447	4.04E-59	-1.69E-46
MS.gene053599	MS.gene82546	0.800717	1.26E-48	-1.69E-46
MS.gene05364	MS.gene82546	0.830103	3.55E-55	-1.69E-46
MS.gene054142	MS.gene82546	0.801466	8.89E-49	-1.69E-46
MS.gene054584	MS.gene82546	0.813504	2.48E-51	-1.69E-46
MS.gene055144	MS.gene82546	0.803996	2.67E-49	-1.69E-46
MS.gene055465	MS.gene82546	0.842084	3.21E-58	-1.69E-46
MS.gene055466	MS.gene82546	0.82404	1.00E-53	-1.69E-46
MS.gene055468	MS.gene82546	0.826511	2.61E-54	-1.69E-46
MS.gene055469	MS.gene82546	0.8196	1.07E-52	-1.69E-46
MS.gene055537	MS.gene82546	0.819432	1.16E-52	-1.69E-46
MS.gene055580	MS.gene82546	-0.812385	4.35E-51	-1.69E-46
MS.gene05608	MS.gene82546	0.837455	5.15E-57	-1.69E-46
MS.gene056169	MS.gene82546	0.80738	5.20E-50	-1.69E-46
MS.gene056215	MS.gene82546	0.86012	2.58E-63	-1.69E-46
MS.gene056308	MS.gene82546	0.830866	2.31E-55	-1.69E-46
MS.gene057082	MS.gene82546	0.803836	2.88E-49	-1.69E-46
MS.gene057294	MS.gene82546	0.821281	4.39E-53	-1.69E-46
MS.gene057915	MS.gene82546	0.803659	3.14E-49	-1.69E-46
MS.gene05847	MS.gene82546	0.80267	5.02E-49	-1.69E-46
MS.gene05870	MS.gene82546	0.813467	2.52E-51	-1.69E-46
MS.gene05894	MS.gene82546	0.81824	2.17E-52	-1.69E-46
MS.gene059300	MS.gene82546	0.811499	6.79E-51	-1.69E-46
MS.gene059625	MS.gene82546	0.805339	1.40E-49	-1.69E-46
MS.gene059778	MS.gene82546	0.820239	7.62E-53	-1.69E-46
MS.gene05978	MS.gene82546	0.808925	2.44E-50	-1.69E-46
MS.gene060429	MS.gene82546	0.818479	1.92E-52	-1.69E-46
MS.gene060519	MS.gene82546	0.853606	2.14E-61	-1.69E-46
MS.gene060587	MS.gene82546	0.832916	7.19E-56	-1.69E-46
MS.gene061100	MS.gene82546	0.825347	4.92E-54	-1.69E-46
MS.gene061178	MS.gene82546	0.800057	1.72E-48	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene08121	MS.gene82546	PPI
MS.gene061870	MS.gene82546	PPI
MS.gene82546	MS.gene035720	PPI
MS.gene035718	MS.gene82546	PPI
MS.gene035896	MS.gene82546	PPI
MS.gene82546	MS.gene049705	PPI
MS.gene009027	MS.gene82546	PPI
MS.gene82546	MS.gene070907	PPI
MS.gene78578	MS.gene82546	PPI
MS.gene035721	MS.gene82546	PPI
MS.gene82546	MS.gene010219	PPI
MS.gene82546	MS.gene05608	PPI
MS.gene08122	MS.gene82546	PPI
MS.gene82546	MS.gene68545	PPI
MS.gene05623	MS.gene82546	PPI
MS.gene043967	MS.gene82546	PPI
MS.gene028629	MS.gene82546	PPI
MS.gene04433	MS.gene82546	PPI
MS.gene82546	MS.gene08122	PPI
MS.gene035787	MS.gene82546	PPI
MS.gene04067	MS.gene82546	PPI
MS.gene71215	MS.gene82546	PPI
MS.gene82546	MS.gene09873	PPI
MS.gene035719	MS.gene82546	PPI
MS.gene58854	MS.gene82546	PPI
MS.gene82546	MS.gene62520	PPI
MS.gene66225	MS.gene82546	PPI
MS.gene70955	MS.gene82546	PPI
MS.gene071136	MS.gene82546	PPI
MS.gene040383	MS.gene82546	PPI
MS.gene070242	MS.gene82546	PPI
MS.gene072493	MS.gene82546	PPI
MS.gene048746	MS.gene82546	PPI
MS.gene010220	MS.gene82546	PPI
MS.gene82546	MS.gene004124	PPI
MS.gene79195	MS.gene82546	PPI
MS.gene88680	MS.gene82546	PPI
MS.gene056215	MS.gene82546	PPI
MS.gene064557	MS.gene82546	PPI
MS.gene049821	MS.gene82546	PPI
MS.gene038469	MS.gene82546	PPI
MS.gene82546	MS.gene04052	PPI
MS.gene82546	MS.gene84536	PPI
MS.gene82546	MS.gene061866	PPI
MS.gene04452	MS.gene82546	PPI
MS.gene82546	MS.gene64650	PPI
MS.gene82546	MS.gene60093	PPI
MS.gene82546	MS.gene061874	PPI
MS.gene09871	MS.gene82546	PPI
MS.gene82546	MS.gene061869	PPI
MS.gene89487	MS.gene82546	PPI
MS.gene82546	MS.gene04972	PPI
MS.gene82546	MS.gene004121	PPI
MS.gene04392	MS.gene82546	PPI
MS.gene82546	MS.gene018578	PPI
MS.gene30920	MS.gene82546	PPI
MS.gene08053	MS.gene82546	PPI
MS.gene061872	MS.gene82546	PPI
MS.gene82546	MS.gene061867	PPI
MS.gene82546	MS.gene04064	PPI
MS.gene82546	MS.gene018580	PPI
MS.gene04068	MS.gene82546	PPI
MS.gene82546	MS.gene008276	PPI
MS.gene82546	MS.gene05619	PPI
MS.gene070241	MS.gene82546	PPI
MS.gene04434	MS.gene82546	PPI
MS.gene022946	MS.gene82546	PPI
MS.gene04064	MS.gene82546	PPI
MS.gene82546	MS.gene89488	PPI
MS.gene061876	MS.gene82546	PPI
MS.gene022932	MS.gene82546	PPI
MS.gene82546	MS.gene048705	PPI
MS.gene82546	MS.gene61017	PPI
MS.gene012391	MS.gene82546	PPI
MS.gene88681	MS.gene82546	PPI
MS.gene82546	MS.gene04576	PPI
MS.gene070244	MS.gene82546	PPI
MS.gene035897	MS.gene82546	PPI
MS.gene64920	MS.gene82546	PPI
MS.gene82546	MS.gene012390	PPI
MS.gene060463	MS.gene82546	PPI
MS.gene070907	MS.gene82546	PPI
MS.gene31196	MS.gene82546	PPI
MS.gene060465	MS.gene82546	PPI
MS.gene82546	MS.gene016624	PPI
MS.gene82546	MS.gene73136	PPI
MS.gene82546	MS.gene049821	PPI

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82546.t1	MTR_7g029510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_7g099610	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_5g063620	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_4g061827	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_4g061920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g462400	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g054380	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g462730	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g462510	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g070920	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g061440	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_3g462410	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_2g096100	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_8g038460	100.000	103	0	1	103	1	103	2.85e-68	199
MS.gene82546.t1	MTR_4g128150	100.000	102	0	1	102	1	102	1.67e-66	204
MS.gene82546.t1	MTR_7g029520	84.000	50	8	27	76	1	50	2.15e-21	87.0

Query id	Subject id	identity %	alignment length	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82546.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT5G59690	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT5G59970	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT3G46320	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT3G45930	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT3G53730	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT2G28740	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT1G07660	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT1G07820	100.000	103	0	1	103	1	103	2.99e-68	199
MS.gene82546.t1	AT1G07660	83.495	103	1	1	103	1	86	4.34e-51	155

Find 37 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGCCTGCGATTCGTCGTT+TGG	0.335029	3.1:-58185771	MS.gene82546:CDS
TTTCATCTCTTGAAAATGTC+TGG	0.354834	3.1:-58185878	None:intergenic
AGGCAGGGAAGAACCCTCTA+TGG	0.361242	3.1:-58185587	MS.gene82546:CDS
GGAAGAACCCTCTATGGGTT+TGG	0.426577	3.1:-58185581	MS.gene82546:CDS
GGAGGTAAGGGACTAGGAAA+GGG	0.443358	3.1:-58185845	MS.gene82546:CDS
ATCAACCTCCAAACCCATAG+AGG	0.443645	3.1:+58185573	None:intergenic
GGGAGGTAAGGGACTAGGAA+AGG	0.449480	3.1:-58185846	MS.gene82546:CDS
TGGTCGTGGTAAGGGAGGTA+AGG	0.462120	3.1:-58185858	MS.gene82546:CDS
TAGGAAAGGGAGGTGCAAAA+AGG	0.467478	3.1:-58185832	MS.gene82546:CDS
GAAAATGTCTGGTCGTGGTA+AGG	0.478730	3.1:-58185867	None:intergenic
TGTCTATGCTTTGAAGAGGC+AGG	0.485721	3.1:-58185603	MS.gene82546:CDS
AAAGATCTTCAACACACCTC+TGG	0.488735	3.1:+58185700	None:intergenic
AGAACCCTCTATGGGTTTGG+AGG	0.501382	3.1:-58185578	MS.gene82546:CDS
CGTCGTTTGGCGAGAAGAGG+TGG	0.504748	3.1:-58185758	MS.gene82546:CDS
GGTGCTTCGCGATAACATCC+AGG	0.510741	3.1:-58185801	MS.gene82546:CDS
CTCGCCAAACGACGAATCGC+AGG	0.516571	3.1:+58185767	None:intergenic
GGTAAGGGAGGTAAGGGACT+AGG	0.518213	3.1:-58185851	MS.gene82546:CDS
CAAACGACGAATCGCAGGCT+TGG	0.524661	3.1:+58185772	None:intergenic
AAAATGTCTGGTCGTGGTAA+GGG	0.526072	3.1:-58185866	MS.gene82546:CDS
GTCTATGCTTTGAAGAGGCA+GGG	0.532756	3.1:-58185602	MS.gene82546:CDS
ATTCGTCGTTTGGCGAGAAG+AGG	0.560388	3.1:-58185761	MS.gene82546:CDS
CTCTTGAAAATGTCTGGTCG+TGG	0.562247	3.1:-58185872	None:intergenic
GGTAAGGGACTAGGAAAGGG+AGG	0.563878	3.1:-58185842	MS.gene82546:CDS
GAGGAAGACAGTTACCGCTA+TGG	0.566121	3.1:-58185630	MS.gene82546:CDS
CGAGAAGAGGTGGTGTGAAG+AGG	0.574990	3.1:-58185748	MS.gene82546:CDS
TTACATACACTGAGCATGCA+AGG	0.586915	3.1:-58185652	MS.gene82546:CDS
TCGCAGGCTTGGTGATACCC+TGG	0.588643	3.1:+58185783	None:intergenic
GGCAGGGAAGAACCCTCTAT+GGG	0.597151	3.1:-58185586	MS.gene82546:CDS
GGTCGTGGTAAGGGAGGTAA+GGG	0.613033	3.1:-58185857	MS.gene82546:CDS
TCAACCTCCAAACCCATAGA+GGG	0.633837	3.1:+58185574	None:intergenic
ATGTCTGGTCGTGGTAAGGG+AGG	0.640958	3.1:-58185863	MS.gene82546:CDS
GGTGGTGTGAAGAGGATCAG+TGG	0.651491	3.1:-58185740	MS.gene82546:CDS
GTGCTTCGCGATAACATCCA+GGG	0.673472	3.1:-58185800	MS.gene82546:CDS
ATGTTGTCTATGCTTTGAAG+AGG	0.684273	3.1:-58185607	MS.gene82546:CDS
AGCATAGACAACATCCATAG+CGG	0.715505	3.1:+58185616	None:intergenic
CTGATCTATGAAGAAACCAG+AGG	0.720815	3.1:-58185716	MS.gene82546:CDS
CATACACTGAGCATGCAAGG+AGG	0.758591	3.1:-58185649	MS.gene82546:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGGTGTGTTGAAGATCTTTT+TGG	-	chr3.1:58185741-58185760	MS.gene82546:CDS	35.0%
!!	ATGTTGTCTATGCTTTGAAG+AGG	-	chr3.1:58185830-58185849	MS.gene82546:CDS	35.0%
	AAAGATCTTCAACACACCTC+TGG	+	chr3.1:58185740-58185759	None:intergenic	40.0%
	AGCATAGACAACATCCATAG+CGG	+	chr3.1:58185824-58185843	None:intergenic	40.0%
	CTGATCTATGAAGAAACCAG+AGG	-	chr3.1:58185721-58185740	MS.gene82546:CDS	40.0%
	TTACATACACTGAGCATGCA+AGG	-	chr3.1:58185785-58185804	MS.gene82546:CDS	40.0%
	TCAACCTCCAAACCCATAGA+GGG	+	chr3.1:58185866-58185885	None:intergenic	45.0%
!	AGGTGCAAAAAGGCATAGGA+AGG	-	chr3.1:58185615-58185634	MS.gene82546:CDS	45.0%
!	TAGGAAAGGGAGGTGCAAAA+AGG	-	chr3.1:58185605-58185624	MS.gene82546:CDS	45.0%
!!	GTCTATGCTTTGAAGAGGCA+GGG	-	chr3.1:58185835-58185854	MS.gene82546:CDS	45.0%
!!	TGTCTATGCTTTGAAGAGGC+AGG	-	chr3.1:58185834-58185853	MS.gene82546:CDS	45.0%
	AGAACCCTCTATGGGTTTGG+AGG	-	chr3.1:58185859-58185878	MS.gene82546:CDS	50.0%
	ATTCGTCGTTTGGCGAGAAG+AGG	-	chr3.1:58185676-58185695	MS.gene82546:CDS	50.0%
	CATACACTGAGCATGCAAGG+AGG	-	chr3.1:58185788-58185807	MS.gene82546:CDS	50.0%
	GAGGAAGACAGTTACCGCTA+TGG	-	chr3.1:58185807-58185826	MS.gene82546:CDS	50.0%
	GGAAGAACCCTCTATGGGTT+TGG	-	chr3.1:58185856-58185875	MS.gene82546:CDS	50.0%
	GGAGGTAAGGGACTAGGAAA+GGG	-	chr3.1:58185592-58185611	MS.gene82546:CDS	50.0%
	GTGCTTCGCGATAACATCCA+GGG	-	chr3.1:58185637-58185656	MS.gene82546:CDS	50.0%
!	AGGGAGGTGCAAAAAGGCAT+AGG	-	chr3.1:58185611-58185630	MS.gene82546:CDS	50.0%
	AGGCAGGGAAGAACCCTCTA+TGG	-	chr3.1:58185850-58185869	MS.gene82546:CDS	55.0%
	ATGTCTGGTCGTGGTAAGGG+AGG	-	chr3.1:58185574-58185593	MS.gene82546:CDS	55.0%
	CAAACGACGAATCGCAGGCT+TGG	+	chr3.1:58185668-58185687	None:intergenic	55.0%
	CAAGCCTGCGATTCGTCGTT+TGG	-	chr3.1:58185666-58185685	MS.gene82546:CDS	55.0%
	CGAGAAGAGGTGGTGTGAAG+AGG	-	chr3.1:58185689-58185708	MS.gene82546:CDS	55.0%
	GGCAGGGAAGAACCCTCTAT+GGG	-	chr3.1:58185851-58185870	MS.gene82546:CDS	55.0%
	GGGAGGTAAGGGACTAGGAA+AGG	-	chr3.1:58185591-58185610	MS.gene82546:CDS	55.0%
	GGTAAGGGACTAGGAAAGGG+AGG	-	chr3.1:58185595-58185614	MS.gene82546:CDS	55.0%
	GGTAAGGGAGGTAAGGGACT+AGG	-	chr3.1:58185586-58185605	MS.gene82546:CDS	55.0%
	GGTCGTGGTAAGGGAGGTAA+GGG	-	chr3.1:58185580-58185599	MS.gene82546:CDS	55.0%
	GGTGCTTCGCGATAACATCC+AGG	-	chr3.1:58185636-58185655	MS.gene82546:CDS	55.0%
	GGTGGTGTGAAGAGGATCAG+TGG	-	chr3.1:58185697-58185716	MS.gene82546:CDS	55.0%
	TGGTCGTGGTAAGGGAGGTA+AGG	-	chr3.1:58185579-58185598	MS.gene82546:CDS	55.0%
	CGTCGTTTGGCGAGAAGAGG+TGG	-	chr3.1:58185679-58185698	MS.gene82546:CDS	60.0%
	CTCGCCAAACGACGAATCGC+AGG	+	chr3.1:58185673-58185692	None:intergenic	60.0%
!!	TCGCAGGCTTGGTGATACCC+TGG	+	chr3.1:58185657-58185676	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	58185574	58185885	58185574	ID=MS.gene82546
chr3.1	mRNA	58185574	58185885	58185574	ID=MS.gene82546.t1;Parent=MS.gene82546
chr3.1	exon	58185574	58185885	58185574	ID=MS.gene82546.t1.exon1;Parent=MS.gene82546.t1
chr3.1	CDS	58185574	58185885	58185574	ID=cds.MS.gene82546.t1;Parent=MS.gene82546.t1

Gene Sequence	>MS.gene82546 ATGTCTGGTCGTGGTAAGGGAGGTAAGGGACTAGGAAAGGGAGGTGCAAAAAGGCATAGGAAGGTGCTTCGCGATAACATCCAGGGTATCACCAAGCCTGCGATTCGTCGTTTGGCGAGAAGAGGTGGTGTGAAGAGGATCAGTGGTCTGATCTATGAAGAAACCAGAGGTGTGTTGAAGATCTTTTTGGAGAATGTCATTCGTGATGCAGTTACATACACTGAGCATGCAAGGAGGAAGACAGTTACCGCTATGGATGTTGTCTATGCTTTGAAGAGGCAGGGAAGAACCCTCTATGGGTTTGGAGGTTGA
Protein sequence	>MS.gene82546.t1 MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG