Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059672.t1 | XP_003611473.1 | 100 | 438 | 0 | 0 | 1 | 438 | 1 | 438 | 7.60E-251 | 875.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059672.t1 | O23140 | 94.3 | 438 | 25 | 0 | 1 | 438 | 1 | 438 | 2.1e-241 | 835.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059672.t1 | G7JZX7 | 100.0 | 438 | 0 | 0 | 1 | 438 | 1 | 438 | 5.5e-251 | 875.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene072572 | MS.gene059672 | PPI |
| MS.gene059672 | MS.gene008864 | PPI |
| MS.gene059672 | MS.gene010105 | PPI |
| MS.gene050001 | MS.gene059672 | PPI |
| MS.gene059672 | MS.gene050768 | PPI |
| MS.gene059672 | MS.gene054375 | PPI |
| MS.gene050771 | MS.gene059672 | PPI |
| MS.gene29199 | MS.gene059672 | PPI |
| MS.gene86178 | MS.gene059672 | PPI |
| MS.gene067937 | MS.gene059672 | PPI |
| MS.gene23142 | MS.gene059672 | PPI |
| MS.gene059672 | MS.gene44324 | PPI |
| MS.gene068109 | MS.gene059672 | PPI |
| MS.gene027687 | MS.gene059672 | PPI |
| MS.gene059672 | MS.gene056431 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059672.t1 | MTR_5g014330 | 100.000 | 438 | 0 | 0 | 1 | 438 | 1 | 438 | 0.0 | 901 |
| MS.gene059672.t1 | MTR_5g014330 | 100.000 | 398 | 0 | 0 | 1 | 398 | 1 | 398 | 0.0 | 817 |
| MS.gene059672.t1 | MTR_8g069565 | 44.091 | 440 | 229 | 7 | 1 | 437 | 1 | 426 | 2.45e-125 | 370 |
| MS.gene059672.t1 | MTR_5g033500 | 43.409 | 440 | 232 | 6 | 1 | 437 | 1 | 426 | 5.41e-125 | 369 |
| MS.gene059672.t1 | MTR_5g033500 | 42.724 | 323 | 169 | 5 | 1 | 321 | 1 | 309 | 7.35e-86 | 267 |
| MS.gene059672.t1 | MTR_3g112190 | 32.230 | 453 | 274 | 9 | 6 | 438 | 3 | 442 | 1.03e-77 | 248 |
| MS.gene059672.t1 | MTR_5g066050 | 32.969 | 458 | 273 | 10 | 6 | 438 | 3 | 451 | 1.24e-74 | 241 |
| MS.gene059672.t1 | MTR_5g066050 | 32.898 | 459 | 271 | 12 | 6 | 438 | 3 | 450 | 7.53e-74 | 238 |
| MS.gene059672.t1 | MTR_7g111870 | 29.315 | 365 | 220 | 11 | 81 | 437 | 76 | 410 | 2.04e-44 | 160 |
| MS.gene059672.t1 | MTR_7g111870 | 31.395 | 258 | 145 | 7 | 81 | 330 | 76 | 309 | 2.08e-36 | 136 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059672.t1 | AT5G46630 | 94.292 | 438 | 25 | 0 | 1 | 438 | 1 | 438 | 0.0 | 859 |
| MS.gene059672.t1 | AT5G46630 | 93.947 | 413 | 25 | 0 | 1 | 413 | 1 | 413 | 0.0 | 805 |
| MS.gene059672.t1 | AT1G60780 | 42.500 | 440 | 236 | 7 | 1 | 437 | 1 | 426 | 1.54e-122 | 363 |
| MS.gene059672.t1 | AT1G10730 | 42.500 | 440 | 236 | 7 | 1 | 437 | 1 | 426 | 6.49e-120 | 356 |
| MS.gene059672.t1 | AT4G24550 | 32.898 | 459 | 271 | 8 | 6 | 438 | 4 | 451 | 1.17e-82 | 261 |
| MS.gene059672.t1 | AT4G24550 | 32.316 | 393 | 230 | 7 | 6 | 373 | 4 | 385 | 1.40e-65 | 215 |
| MS.gene059672.t1 | AT4G24550 | 32.461 | 382 | 222 | 7 | 6 | 362 | 4 | 374 | 5.61e-65 | 213 |
| MS.gene059672.t1 | AT1G56590 | 28.255 | 361 | 229 | 8 | 81 | 437 | 80 | 414 | 1.97e-40 | 149 |
Find 88 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTATTCAAATCATATTT+TGG | 0.157391 | 5.1:-7120363 | MS.gene059672:CDS |
| ACCTATTCAGATGGAGTTTC+AGG | 0.250537 | 5.1:-7114491 | MS.gene059672:intron |
| CTTATGAAGTGCTTTCTATC+TGG | 0.279752 | 5.1:-7117610 | MS.gene059672:CDS |
| AAACTAACCTTGGATGAAAA+AGG | 0.282659 | 5.1:+7118317 | None:intergenic |
| TTAAATCACGCCCTACTAAA+AGG | 0.294099 | 5.1:-7117528 | MS.gene059672:intron |
| GTTGCTTTACAGGGGAAATA+TGG | 0.297064 | 5.1:-7120795 | MS.gene059672:intron |
| TGTTCGACCAAGTTCCTTGA+TGG | 0.315007 | 5.1:+7115108 | None:intergenic |
| CAGCTATTGATTGTTTAGTT+TGG | 0.318085 | 5.1:-7114854 | MS.gene059672:CDS |
| TTTCAGGTAAAGAGTGTCTT+TGG | 0.322597 | 5.1:-7114978 | MS.gene059672:intron |
| TCGTCAAATTTACACATTGA+TGG | 0.324003 | 5.1:+7117227 | None:intergenic |
| GTAACTTGTAACGTCGCATT+TGG | 0.335147 | 5.1:+7117914 | None:intergenic |
| GTCTCTATCGCGACGATGTC+GGG | 0.365747 | 5.1:-7121889 | MS.gene059672:intron |
| GTTCCCATGTTCACAGCATC+TGG | 0.373702 | 5.1:-7114012 | MS.gene059672:CDS |
| CTTATTTACGAGCTTCTAGA+TGG | 0.387111 | 5.1:-7120300 | MS.gene059672:intron |
| ATCAGTTCAAATTCACCATC+TGG | 0.398480 | 5.1:+7117169 | None:intergenic |
| TTCAAACCCAACTTCAAATC+AGG | 0.422092 | 5.1:+7117583 | None:intergenic |
| CTGAAGGTGTGGGAAAAGAG+TGG | 0.425118 | 5.1:-7113880 | MS.gene059672:intron |
| GATGGTTGGCAATACTTTGA+AGG | 0.426750 | 5.1:+7115126 | None:intergenic |
| TGCTTTCAAGTTTGTTGTAG+AGG | 0.429659 | 5.1:-7120627 | MS.gene059672:intron |
| AAATGCGACGTTACAAGTTA+CGG | 0.430314 | 5.1:-7117912 | MS.gene059672:CDS |
| GGAATGCCTGATTTGAAGTT+GGG | 0.447084 | 5.1:-7117589 | MS.gene059672:CDS |
| TTTGCAGAAATCATGGACTT+TGG | 0.450042 | 5.1:-7118404 | MS.gene059672:intron |
| TCACACTCTCCACAATATCT+AGG | 0.454091 | 5.1:+7117758 | None:intergenic |
| TTGGGTTTGAATGACAAGAT+TGG | 0.457575 | 5.1:-7117571 | MS.gene059672:CDS |
| TGTAACGTCGCATTTGGAAC+AGG | 0.460744 | 5.1:+7117920 | None:intergenic |
| CGATGACTGAAAAGAAATCA+TGG | 0.469490 | 5.1:-7114522 | MS.gene059672:CDS |
| CGTCTCTATCGCGACGATGT+CGG | 0.469885 | 5.1:-7121890 | MS.gene059672:CDS |
| AAGCAGGATCATATGAGATT+AGG | 0.476592 | 5.1:-7113822 | MS.gene059672:CDS |
| ACCTGAAACTCCATCTGAAT+AGG | 0.479414 | 5.1:+7114490 | None:intergenic |
| TCAATTTCTGCGCTCAAGGT+TGG | 0.484700 | 5.1:+7114556 | None:intergenic |
| TGGAATGCCTGATTTGAAGT+TGG | 0.486730 | 5.1:-7117590 | MS.gene059672:CDS |
| AATGCGACGTTACAAGTTAC+GGG | 0.487086 | 5.1:-7117911 | MS.gene059672:CDS |
| GCACGTATTTGCAGAAATCA+TGG | 0.488215 | 5.1:-7118411 | MS.gene059672:intron |
| ACGGATGGAAGTAAATGTTA+AGG | 0.488849 | 5.1:-7115086 | MS.gene059672:intron |
| CATCCACCAATCTGCTTCAC+AGG | 0.489532 | 5.1:+7120722 | None:intergenic |
| AATCTTCTTATGTCGTCAAA+AGG | 0.490176 | 5.1:-7117736 | MS.gene059672:intron |
| TGGGTACAACACTGTTGAAT+GGG | 0.491741 | 5.1:-7113860 | MS.gene059672:CDS |
| GCTGTTGGTTGGCGGAGAGA+AGG | 0.495289 | 5.1:-7117887 | MS.gene059672:CDS |
| AAGGTTGGCTCAGTTTGCCC+AGG | 0.499168 | 5.1:+7114571 | None:intergenic |
| CGACCAAGTTCCTTGATGGT+TGG | 0.502376 | 5.1:+7115112 | None:intergenic |
| GTGGGTACAACACTGTTGAA+TGG | 0.504729 | 5.1:-7113861 | MS.gene059672:CDS |
| AAGTTACGGGTGCTGTTGGT+TGG | 0.507828 | 5.1:-7117898 | MS.gene059672:CDS |
| TAAACCAGATGCTGTGAACA+TGG | 0.514088 | 5.1:+7114008 | None:intergenic |
| AAGCTTTATATCACTCAAGA+AGG | 0.514636 | 5.1:-7118350 | MS.gene059672:CDS |
| TTGCCAACCATCAAGGAACT+TGG | 0.515329 | 5.1:-7115115 | MS.gene059672:CDS |
| TGACAGATACCTTAAGAAAA+CGG | 0.516213 | 5.1:+7113980 | None:intergenic |
| AAGTTCAATTTCTGCGCTCA+AGG | 0.518053 | 5.1:+7114552 | None:intergenic |
| ACCAATCTGCTTCACAGGAC+AGG | 0.518339 | 5.1:+7120727 | None:intergenic |
| ATATAAAGCTTTAAGATCTC+CGG | 0.519663 | 5.1:+7118362 | None:intergenic |
| GTTCTGCGCTGTGATGTAAC+GGG | 0.519953 | 5.1:-7117640 | MS.gene059672:CDS |
| TTACGGGTGCTGTTGGTTGG+CGG | 0.523294 | 5.1:-7117895 | MS.gene059672:CDS |
| CAAAGTATTGCCAACCATCA+AGG | 0.525550 | 5.1:-7115122 | MS.gene059672:CDS |
| GTGCAAAGATGTTTGCACTA+GGG | 0.528491 | 5.1:-7114956 | MS.gene059672:CDS |
| TGTTCTGCGCTGTGATGTAA+CGG | 0.532251 | 5.1:-7117641 | MS.gene059672:CDS |
| ATGGTTGGCAATACTTTGAA+GGG | 0.536477 | 5.1:+7115127 | None:intergenic |
| ACCTGTCCTGTGAAGCAGAT+TGG | 0.537523 | 5.1:-7120728 | MS.gene059672:CDS |
| ACAGGTGTTCCTAGATATTG+TGG | 0.545898 | 5.1:-7117767 | MS.gene059672:intron |
| GGTGCAAAGATGTTTGCACT+AGG | 0.547993 | 5.1:-7114957 | MS.gene059672:CDS |
| ACGAGTTTCACAGTCACATC+CGG | 0.553418 | 5.1:-7114894 | MS.gene059672:CDS |
| ATAATGCAAACGAAAGAACT+TGG | 0.553697 | 5.1:-7120752 | MS.gene059672:CDS |
| GACTAGACCACCTATTCAGA+TGG | 0.555206 | 5.1:-7114500 | MS.gene059672:CDS |
| TGCAGGATAAGAAAATTCCC+TGG | 0.556238 | 5.1:-7114589 | MS.gene059672:intron |
| TGCAAAGATGTTTGCACTAG+GGG | 0.556537 | 5.1:-7114955 | MS.gene059672:CDS |
| ATCAGGTATCGTATCACTGA+GGG | 0.564329 | 5.1:-7115160 | MS.gene059672:intron |
| TAAGTTGTGACTCTTTCTCA+AGG | 0.567568 | 5.1:+7117548 | None:intergenic |
| AGCACAAGCTACATTAGCAT+TGG | 0.569713 | 5.1:+7120646 | None:intergenic |
| TTATCCACAAAATCTTTCAC+CGG | 0.572212 | 5.1:-7118381 | MS.gene059672:CDS |
| TAAACAGCGGAAGCAGCCAC+CGG | 0.577176 | 5.1:+7121947 | None:intergenic |
| TTACAAGTTACGGGTGCTGT+TGG | 0.579978 | 5.1:-7117902 | MS.gene059672:CDS |
| GTTCGCTATATCACAAAAGC+AGG | 0.583623 | 5.1:-7113838 | MS.gene059672:CDS |
| TGTCCTGTGAAGCAGATTGG+TGG | 0.590779 | 5.1:-7120725 | MS.gene059672:CDS |
| GGAACTTGGTCGAACACGGA+TGG | 0.592221 | 5.1:-7115101 | MS.gene059672:CDS |
| GCAGGATAAGAAAATTCCCT+GGG | 0.598297 | 5.1:-7114588 | MS.gene059672:intron |
| AGGTTGGCTCAGTTTGCCCA+GGG | 0.608851 | 5.1:+7114572 | None:intergenic |
| TCAAGGAACTTGGTCGAACA+CGG | 0.609772 | 5.1:-7115105 | MS.gene059672:CDS |
| AAATTTGACGAGGTTCAACT+CGG | 0.616156 | 5.1:-7117215 | MS.gene059672:CDS |
| TGAAGGTGTGGGAAAAGAGT+GGG | 0.616743 | 5.1:-7113879 | MS.gene059672:intron |
| TGAAACTCCATCTGAATAGG+TGG | 0.625115 | 5.1:+7114493 | None:intergenic |
| GTTTACTTCTTGAACCTCCG+TGG | 0.631442 | 5.1:-7121929 | MS.gene059672:CDS |
| ATCAATGTGTAAATTTGACG+AGG | 0.649503 | 5.1:-7117225 | MS.gene059672:CDS |
| CACTGGATCGGAGATGCCGG+TGG | 0.655709 | 5.1:-7121963 | None:intergenic |
| AAACCAGATGCTGTGAACAT+GGG | 0.662215 | 5.1:+7114009 | None:intergenic |
| GATTGATGAGAACGTCACCA+CGG | 0.673884 | 5.1:+7121912 | None:intergenic |
| AGTTCAAATTCACCATCTGG+AGG | 0.689739 | 5.1:+7117172 | None:intergenic |
| TTCTGCGCTGTGATGTAACG+GGG | 0.698309 | 5.1:-7117639 | MS.gene059672:CDS |
| TATCAGGTATCGTATCACTG+AGG | 0.719317 | 5.1:-7115161 | MS.gene059672:intron |
| TGATGAGAACGTCACCACGG+AGG | 0.731384 | 5.1:+7121915 | None:intergenic |
| GAGGTTCAAGAAGTAAACAG+CGG | 0.750495 | 5.1:+7121934 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 7113816 | 7121972 | 7113816 | ID=MS.gene059672 |
| chr5.1 | mRNA | 7113816 | 7121972 | 7113816 | ID=MS.gene059672.t1;Parent=MS.gene059672 |
| chr5.1 | exon | 7121890 | 7121972 | 7121890 | ID=MS.gene059672.t1.exon1;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7121890 | 7121972 | 7121890 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7120628 | 7120805 | 7120628 | ID=MS.gene059672.t1.exon2;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7120628 | 7120805 | 7120628 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7120301 | 7120391 | 7120301 | ID=MS.gene059672.t1.exon3;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7120301 | 7120391 | 7120301 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7118325 | 7118419 | 7118325 | ID=MS.gene059672.t1.exon4;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7118325 | 7118419 | 7118325 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7117865 | 7117954 | 7117865 | ID=MS.gene059672.t1.exon5;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7117865 | 7117954 | 7117865 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7117737 | 7117785 | 7117737 | ID=MS.gene059672.t1.exon6;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7117737 | 7117785 | 7117737 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7117529 | 7117667 | 7117529 | ID=MS.gene059672.t1.exon7;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7117529 | 7117667 | 7117529 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7117166 | 7117282 | 7117166 | ID=MS.gene059672.t1.exon8;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7117166 | 7117282 | 7117166 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7115087 | 7115177 | 7115087 | ID=MS.gene059672.t1.exon9;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7115087 | 7115177 | 7115087 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7114852 | 7114994 | 7114852 | ID=MS.gene059672.t1.exon10;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7114852 | 7114994 | 7114852 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7114492 | 7114606 | 7114492 | ID=MS.gene059672.t1.exon11;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7114492 | 7114606 | 7114492 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7113990 | 7114034 | 7113990 | ID=MS.gene059672.t1.exon12;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7113990 | 7114034 | 7113990 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| chr5.1 | exon | 7113816 | 7113896 | 7113816 | ID=MS.gene059672.t1.exon13;Parent=MS.gene059672.t1 |
| chr5.1 | CDS | 7113816 | 7113896 | 7113816 | ID=cds.MS.gene059672.t1;Parent=MS.gene059672.t1 |
| Gene Sequence |
| Protein sequence |
