Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050001.t1 | XP_003615276.1 | 99.8 | 451 | 1 | 0 | 1 | 451 | 1 | 451 | 2.60E-254 | 887.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050001.t1 | Q9SB50 | 82.5 | 451 | 78 | 1 | 1 | 451 | 2 | 451 | 3.5e-215 | 748.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050001.t1 | G7KBJ9 | 99.8 | 451 | 1 | 0 | 1 | 451 | 1 | 451 | 1.9e-254 | 887.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050001 | MS.gene07038 | 0.800076 | 1.71E-48 | -1.69E-46 |
| MS.gene050001 | MS.gene25686 | 0.806322 | 8.70E-50 | -1.69E-46 |
| MS.gene050001 | MS.gene55063 | 0.826191 | 3.11E-54 | -1.69E-46 |
| MS.gene050001 | MS.gene61288 | 0.81117 | 8.01E-51 | -1.69E-46 |
| MS.gene050001 | MS.gene75781 | 0.819109 | 1.38E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene050001 | MS.gene21470 | PPI |
| MS.gene050001 | MS.gene056431 | PPI |
| MS.gene29199 | MS.gene050001 | PPI |
| MS.gene050001 | MS.gene059672 | PPI |
| MS.gene23142 | MS.gene050001 | PPI |
| MS.gene068109 | MS.gene050001 | PPI |
| MS.gene050001 | MS.gene008864 | PPI |
| MS.gene050001 | MS.gene050768 | PPI |
| MS.gene027687 | MS.gene050001 | PPI |
| MS.gene050001 | MS.gene067937 | PPI |
| MS.gene015312 | MS.gene050001 | PPI |
| MS.gene050001 | MS.gene050771 | PPI |
| MS.gene050001 | MS.gene054375 | PPI |
| MS.gene050001 | MS.gene44324 | PPI |
| MS.gene050001 | MS.gene86178 | PPI |
| MS.gene050001 | MS.gene09656 | PPI |
| MS.gene050001 | MS.gene072572 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050001.t1 | MTR_5g066050 | 99.778 | 451 | 1 | 0 | 1 | 451 | 1 | 451 | 0.0 | 922 |
| MS.gene050001.t1 | MTR_5g066050 | 99.557 | 451 | 1 | 1 | 1 | 451 | 1 | 450 | 0.0 | 915 |
| MS.gene050001.t1 | MTR_3g112190 | 80.044 | 451 | 81 | 3 | 1 | 451 | 1 | 442 | 0.0 | 751 |
| MS.gene050001.t1 | MTR_8g069565 | 34.590 | 451 | 264 | 11 | 3 | 451 | 6 | 427 | 7.34e-84 | 264 |
| MS.gene050001.t1 | MTR_5g033500 | 33.481 | 451 | 269 | 11 | 3 | 451 | 6 | 427 | 3.00e-79 | 252 |
| MS.gene050001.t1 | MTR_5g014330 | 33.188 | 458 | 272 | 11 | 3 | 451 | 6 | 438 | 6.75e-77 | 246 |
| MS.gene050001.t1 | MTR_5g014330 | 31.765 | 425 | 257 | 10 | 3 | 418 | 6 | 406 | 5.04e-64 | 212 |
| MS.gene050001.t1 | MTR_5g033500 | 35.294 | 323 | 184 | 8 | 3 | 324 | 6 | 304 | 9.50e-58 | 194 |
| MS.gene050001.t1 | MTR_7g111870 | 30.000 | 340 | 205 | 6 | 1 | 338 | 1 | 309 | 6.93e-43 | 154 |
| MS.gene050001.t1 | MTR_7g111870 | 29.443 | 377 | 226 | 8 | 1 | 375 | 1 | 339 | 7.91e-43 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050001.t1 | AT4G24550 | 82.483 | 451 | 78 | 1 | 1 | 451 | 2 | 451 | 0.0 | 775 |
| MS.gene050001.t1 | AT4G24550 | 81.333 | 375 | 69 | 1 | 1 | 375 | 2 | 375 | 0.0 | 637 |
| MS.gene050001.t1 | AT4G24550 | 81.383 | 376 | 69 | 1 | 1 | 376 | 2 | 376 | 0.0 | 636 |
| MS.gene050001.t1 | AT1G60780 | 34.812 | 451 | 263 | 11 | 3 | 451 | 6 | 427 | 5.84e-82 | 259 |
| MS.gene050001.t1 | AT1G10730 | 35.099 | 453 | 259 | 10 | 3 | 451 | 6 | 427 | 1.39e-81 | 258 |
| MS.gene050001.t1 | AT5G46630 | 32.533 | 458 | 275 | 11 | 3 | 451 | 6 | 438 | 1.87e-75 | 243 |
| MS.gene050001.t1 | AT5G46630 | 31.713 | 432 | 262 | 10 | 3 | 425 | 6 | 413 | 2.29e-67 | 222 |
| MS.gene050001.t1 | AT1G56590 | 27.481 | 393 | 245 | 9 | 1 | 389 | 1 | 357 | 1.59e-37 | 142 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAGAGCTAAGCGAATTTC+AGG | 0.231119 | 5.1:+51190602 | None:intergenic |
| GGTAATATAACGAAAGAATC+TGG | 0.298492 | 5.1:-51185387 | MS.gene050001:CDS |
| TTGCAAATAGGAAAGAAATC+TGG | 0.318770 | 5.1:-51184494 | MS.gene050001:CDS |
| ATGCGAAAAGGAGGCTTAAA+TGG | 0.326302 | 5.1:+51187906 | None:intergenic |
| CAGGTAAAATATTTGCAAAT+AGG | 0.329271 | 5.1:-51184506 | MS.gene050001:CDS |
| TACTTTCATGTGAAAGTTGT+TGG | 0.332550 | 5.1:-51192468 | MS.gene050001:CDS |
| ATGTTTCTAGAGCAGGTTAT+AGG | 0.342498 | 5.1:-51188159 | MS.gene050001:intron |
| GTGCGGTGAAACAGATTAAT+GGG | 0.349197 | 5.1:-51185562 | MS.gene050001:intron |
| TTTCCATGAATCTGTTCGTT+TGG | 0.356258 | 5.1:-51188095 | MS.gene050001:CDS |
| AGTGCGGTGAAACAGATTAA+TGG | 0.360372 | 5.1:-51185563 | MS.gene050001:intron |
| TATTATTTGAACCAGGATTT+CGG | 0.363405 | 5.1:-51191852 | MS.gene050001:intron |
| GCACAATGTATCACAGCTTC+AGG | 0.370690 | 5.1:-51185334 | MS.gene050001:intron |
| TAATACCTGGTGGTATATTT+CGG | 0.378845 | 5.1:+51187400 | None:intergenic |
| CAAATGAAGAGCTATCTTAC+TGG | 0.380878 | 5.1:-51190629 | MS.gene050001:CDS |
| TCTGTTGTTGCAACAGAGCC+TGG | 0.386144 | 5.1:-51191583 | MS.gene050001:CDS |
| AAGATAGCTCTTCATTTGTA+TGG | 0.387761 | 5.1:+51190634 | None:intergenic |
| ATATTGACTTCTGAGATTGA+TGG | 0.388047 | 5.1:-51190659 | MS.gene050001:CDS |
| ATAGTAAATGTCATGCTAAC+AGG | 0.392558 | 5.1:+51185363 | None:intergenic |
| ACATATCTAACCCACCGATA+TGG | 0.403897 | 5.1:+51184461 | None:intergenic |
| TATCCAAACGAACAGATTCA+TGG | 0.414310 | 5.1:+51188092 | None:intergenic |
| AAGGAGGGAGGAAATCTTTG+TGG | 0.417677 | 5.1:-51191554 | MS.gene050001:CDS |
| ACCATGTGATTCCTGAGAAA+AGG | 0.428446 | 5.1:+51185508 | None:intergenic |
| GACTTTGTAACAGCTATTCC+TGG | 0.429404 | 5.1:+51191604 | None:intergenic |
| AAAAGTAGATATCACCTTAA+GGG | 0.429427 | 5.1:+51187857 | None:intergenic |
| GAACTGGAGCCTGGTGCTAC+TGG | 0.430005 | 5.1:-51185717 | MS.gene050001:CDS |
| CTGGGTCGCATGAGCCATAT+CGG | 0.434703 | 5.1:-51184475 | MS.gene050001:CDS |
| AAGTGGCTCATTGAAAATAT+AGG | 0.434746 | 5.1:+51191794 | None:intergenic |
| TACTGTTTGTTGCAACTACA+AGG | 0.435206 | 5.1:-51192443 | MS.gene050001:CDS |
| GCAGCACGTCTGTCACCTCT+TGG | 0.441008 | 5.1:-51191762 | MS.gene050001:CDS |
| GTTTGCTGACAGAATGTAGA+TGG | 0.447416 | 5.1:-51192498 | MS.gene050001:intron |
| CTTCCCAAAACTTTACTTTG+CGG | 0.450669 | 5.1:+51193096 | None:intergenic |
| GTGTTATTAAAGATTACCTT+GGG | 0.455894 | 5.1:-51192371 | MS.gene050001:CDS |
| CGAAGAGGCAGGTTCCCTTA+AGG | 0.468484 | 5.1:-51187871 | MS.gene050001:intron |
| TGGTCCCTCGCTTCTTCCAA+TGG | 0.473383 | 5.1:+51190568 | None:intergenic |
| GAAAAGGAGGCTTAAATGGC+TGG | 0.473779 | 5.1:+51187910 | None:intergenic |
| AATATCTCTGCACTTCCCCT+TGG | 0.476578 | 5.1:+51193122 | None:intergenic |
| ATATCTCTGCACTTCCCCTT+GGG | 0.479873 | 5.1:+51193123 | None:intergenic |
| GCTTAAATGGCTGGGTCATA+CGG | 0.487133 | 5.1:+51187919 | None:intergenic |
| CTGTTGTTGCAACAGAGCCT+GGG | 0.489260 | 5.1:-51191582 | MS.gene050001:CDS |
| GCTAGAATGGAGTTTAAAGA+AGG | 0.489453 | 5.1:-51185661 | MS.gene050001:intron |
| AGGCAAATAAGAAGCTAGAA+TGG | 0.493385 | 5.1:-51185674 | MS.gene050001:CDS |
| TTGGGAAGATGGCGAACTGC+AGG | 0.495134 | 5.1:-51193081 | MS.gene050001:CDS |
| GCAGGTTATAGGAGTTCCTC+TGG | 0.496138 | 5.1:-51188148 | MS.gene050001:intron |
| GACGAAGAACTGTGAGATCA+TGG | 0.497880 | 5.1:+51194215 | None:intergenic |
| ATGACTGGAAATTCACCGTC+TGG | 0.498642 | 5.1:+51187948 | None:intergenic |
| ATCAAAGCATTGATGCGAAA+AGG | 0.498854 | 5.1:+51187894 | None:intergenic |
| CGTGTTATTAAAGATTACCT+TGG | 0.501304 | 5.1:-51192372 | MS.gene050001:CDS |
| TGCAAATAGGAAAGAAATCT+GGG | 0.503739 | 5.1:-51184493 | MS.gene050001:CDS |
| AGAAGCGAGGGACCAATCTC+CGG | 0.516323 | 5.1:-51190560 | MS.gene050001:intron |
| CTTAACGACGATCTTTCCAT+TGG | 0.518614 | 5.1:-51190584 | MS.gene050001:CDS |
| CAGATCGTGGTGAGGTCCCA+AGG | 0.521788 | 5.1:-51193139 | MS.gene050001:CDS |
| TATAGGAGTTCCTCTGGCTC+AGG | 0.522359 | 5.1:-51188142 | MS.gene050001:CDS |
| AAGATAATACCTACAAAGAC+AGG | 0.540202 | 5.1:+51193050 | None:intergenic |
| TTCCTCTGGCTCAGGAGCAG+TGG | 0.541824 | 5.1:-51188134 | MS.gene050001:CDS |
| AGGCAAATTCCTACGTTGCT+CGG | 0.542245 | 5.1:-51184433 | MS.gene050001:CDS |
| CTGGTGGTATATTTCGGTAG+TGG | 0.545193 | 5.1:+51187406 | None:intergenic |
| GTAATATAACGAAAGAATCT+GGG | 0.547603 | 5.1:-51185386 | MS.gene050001:CDS |
| GTCATACGGTAATTCATGAC+TGG | 0.553624 | 5.1:+51187933 | None:intergenic |
| TGTTATCGCCACGCTGTGAA+AGG | 0.565981 | 5.1:+51194193 | None:intergenic |
| CTTACAGAAAAGATCGCAGC+AGG | 0.577072 | 5.1:+51191738 | None:intergenic |
| GAGAGAGAAGCTAACAGTCA+CGG | 0.578733 | 5.1:+51194163 | None:intergenic |
| CTTTCCATTGGAAGAAGCGA+GGG | 0.579543 | 5.1:-51190572 | MS.gene050001:CDS |
| CATCAATGCTTTGATCGAAG+AGG | 0.580364 | 5.1:-51187886 | MS.gene050001:CDS |
| AATGCTTTGATCGAAGAGGC+AGG | 0.581261 | 5.1:-51187882 | MS.gene050001:CDS |
| AACAAACAGAACTCTATCCC+TGG | 0.584190 | 5.1:-51188062 | MS.gene050001:intron |
| AAAAGCTCAAAGACGAAGGA+AGG | 0.598688 | 5.1:+51192408 | None:intergenic |
| CACTACCGAAATATACCACC+AGG | 0.599182 | 5.1:-51187405 | MS.gene050001:intron |
| CTTTATTGCAGATCGTGGTG+AGG | 0.600117 | 5.1:-51193147 | MS.gene050001:intron |
| AAAAGGAGGCTTAAATGGCT+GGG | 0.600629 | 5.1:+51187911 | None:intergenic |
| GGAAGATGGCGAACTGCAGG+AGG | 0.601110 | 5.1:-51193078 | MS.gene050001:CDS |
| TGTTGTTGCAACAGAGCCTG+GGG | 0.604072 | 5.1:-51191581 | MS.gene050001:CDS |
| TACCACTGCTCCTGAGCCAG+AGG | 0.608309 | 5.1:+51188132 | None:intergenic |
| AGTGTTTGCTGTTATACTTG+AGG | 0.609838 | 5.1:+51187444 | None:intergenic |
| TTGTAAAAGCTCAAAGACGA+AGG | 0.612228 | 5.1:+51192404 | None:intergenic |
| GTCGCATGAGCCATATCGGT+GGG | 0.614018 | 5.1:-51184471 | MS.gene050001:CDS |
| TCTTTCCATTGGAAGAAGCG+AGG | 0.615882 | 5.1:-51190573 | MS.gene050001:CDS |
| AGATCGTGGTGAGGTCCCAA+GGG | 0.619776 | 5.1:-51193138 | MS.gene050001:CDS |
| GTTGTCTGTCCAGTAGCACC+AGG | 0.621982 | 5.1:+51185708 | None:intergenic |
| AGCTGTGATACATTGTGCAT+TGG | 0.624337 | 5.1:+51185339 | None:intergenic |
| AAAGCATTGATGCGAAAAGG+AGG | 0.626027 | 5.1:+51187897 | None:intergenic |
| CGTGCTGCATCAATTACAAG+TGG | 0.630367 | 5.1:+51191777 | None:intergenic |
| GGTCGCATGAGCCATATCGG+TGG | 0.633753 | 5.1:-51184472 | MS.gene050001:CDS |
| AAGATCGCAGCAGGTCCAAG+AGG | 0.640400 | 5.1:+51191747 | None:intergenic |
| ACTGTTTGTTGCAACTACAA+GGG | 0.643129 | 5.1:-51192442 | MS.gene050001:CDS |
| TTCTTCGTCCTTTCACAGCG+TGG | 0.643685 | 5.1:-51194201 | MS.gene050001:CDS |
| CATCGAGTAACTCATACACC+AGG | 0.653609 | 5.1:+51192313 | None:intergenic |
| TGCGGTGAAACAGATTAATG+GGG | 0.660205 | 5.1:-51185561 | MS.gene050001:intron |
| AGTCTTCATTGAGAATCCCA+AGG | 0.662867 | 5.1:+51192355 | None:intergenic |
| ACTGGAAATTCACCGTCTGG+AGG | 0.672021 | 5.1:+51187951 | None:intergenic |
| GTGGGTTAGATATGTTACAC+AGG | 0.675410 | 5.1:-51184453 | MS.gene050001:CDS |
| GCATCATATCCGAGCAACGT+AGG | 0.688345 | 5.1:+51184424 | None:intergenic |
| ATAATACCTACAAAGACAGG+TGG | 0.692932 | 5.1:+51193053 | None:intergenic |
| GTTGTTGCAACAGAGCCTGG+GGG | 0.698165 | 5.1:-51191580 | MS.gene050001:CDS |
| GCGGTGAAACAGATTAATGG+GGG | 0.708079 | 5.1:-51185560 | MS.gene050001:intron |
| GATCGTGGTGAGGTCCCAAG+GGG | 0.723226 | 5.1:-51193137 | MS.gene050001:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 51184427 | 51194235 | 51184427 | ID=MS.gene050001 |
| chr5.1 | mRNA | 51184427 | 51194235 | 51184427 | ID=MS.gene050001.t1;Parent=MS.gene050001 |
| chr5.1 | exon | 51194178 | 51194235 | 51194178 | ID=MS.gene050001.t1.exon1;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51194178 | 51194235 | 51194178 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51193064 | 51193158 | 51193064 | ID=MS.gene050001.t1.exon2;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51193064 | 51193158 | 51193064 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51192305 | 51192508 | 51192305 | ID=MS.gene050001.t1.exon3;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51192305 | 51192508 | 51192305 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51191743 | 51191859 | 51191743 | ID=MS.gene050001.t1.exon4;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51191743 | 51191859 | 51191743 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51191516 | 51191644 | 51191516 | ID=MS.gene050001.t1.exon5;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51191516 | 51191644 | 51191516 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51190561 | 51190687 | 51190561 | ID=MS.gene050001.t1.exon6;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51190561 | 51190687 | 51190561 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51188063 | 51188166 | 51188063 | ID=MS.gene050001.t1.exon7;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51188063 | 51188166 | 51188063 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51187872 | 51187976 | 51187872 | ID=MS.gene050001.t1.exon8;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51187872 | 51187976 | 51187872 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51187406 | 51187500 | 51187406 | ID=MS.gene050001.t1.exon9;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51187406 | 51187500 | 51187406 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51185662 | 51185749 | 51185662 | ID=MS.gene050001.t1.exon10;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51185662 | 51185749 | 51185662 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51185510 | 51185570 | 51185510 | ID=MS.gene050001.t1.exon11;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51185510 | 51185570 | 51185510 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51185335 | 51185408 | 51185335 | ID=MS.gene050001.t1.exon12;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51185335 | 51185408 | 51185335 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| chr5.1 | exon | 51184427 | 51184525 | 51184427 | ID=MS.gene050001.t1.exon13;Parent=MS.gene050001.t1 |
| chr5.1 | CDS | 51184427 | 51184525 | 51184427 | ID=cds.MS.gene050001.t1;Parent=MS.gene050001.t1 |
| Gene Sequence |
| Protein sequence |
