Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010105.t1 | XP_003615276.1 | 99.6 | 451 | 2 | 0 | 1 | 451 | 1 | 451 | 9.90E-254 | 885.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010105.t1 | Q9SB50 | 82.3 | 451 | 79 | 1 | 1 | 451 | 2 | 451 | 1.3e-214 | 746.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010105.t1 | G7KBJ9 | 99.6 | 451 | 2 | 0 | 1 | 451 | 1 | 451 | 7.2e-254 | 885.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene86178 | MS.gene010105 | PPI |
| MS.gene067937 | MS.gene010105 | PPI |
| MS.gene21470 | MS.gene010105 | PPI |
| MS.gene23142 | MS.gene010105 | PPI |
| MS.gene027687 | MS.gene010105 | PPI |
| MS.gene44324 | MS.gene010105 | PPI |
| MS.gene015312 | MS.gene010105 | PPI |
| MS.gene072572 | MS.gene010105 | PPI |
| MS.gene059672 | MS.gene010105 | PPI |
| MS.gene054375 | MS.gene010105 | PPI |
| MS.gene09656 | MS.gene010105 | PPI |
| MS.gene050768 | MS.gene010105 | PPI |
| MS.gene29199 | MS.gene010105 | PPI |
| MS.gene068109 | MS.gene010105 | PPI |
| MS.gene056431 | MS.gene010105 | PPI |
| MS.gene050771 | MS.gene010105 | PPI |
| MS.gene008864 | MS.gene010105 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010105.t1 | MTR_5g066050 | 99.557 | 451 | 2 | 0 | 1 | 451 | 1 | 451 | 0.0 | 919 |
| MS.gene010105.t1 | MTR_5g066050 | 99.335 | 451 | 2 | 1 | 1 | 451 | 1 | 450 | 0.0 | 912 |
| MS.gene010105.t1 | MTR_3g112190 | 80.266 | 451 | 80 | 3 | 1 | 451 | 1 | 442 | 0.0 | 755 |
| MS.gene010105.t1 | MTR_8g069565 | 34.368 | 451 | 265 | 11 | 3 | 451 | 6 | 427 | 6.47e-83 | 262 |
| MS.gene010105.t1 | MTR_5g033500 | 33.259 | 451 | 270 | 11 | 3 | 451 | 6 | 427 | 1.64e-78 | 250 |
| MS.gene010105.t1 | MTR_5g014330 | 33.188 | 458 | 272 | 11 | 3 | 451 | 6 | 438 | 2.87e-77 | 248 |
| MS.gene010105.t1 | MTR_5g014330 | 31.765 | 425 | 257 | 10 | 3 | 418 | 6 | 406 | 2.50e-64 | 213 |
| MS.gene010105.t1 | MTR_5g033500 | 34.985 | 323 | 185 | 8 | 3 | 324 | 6 | 304 | 8.12e-57 | 192 |
| MS.gene010105.t1 | MTR_7g111870 | 29.706 | 340 | 206 | 6 | 1 | 338 | 1 | 309 | 5.62e-42 | 152 |
| MS.gene010105.t1 | MTR_7g111870 | 29.178 | 377 | 227 | 8 | 1 | 375 | 1 | 339 | 7.62e-42 | 153 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene010105.t1 | AT4G24550 | 82.262 | 451 | 79 | 1 | 1 | 451 | 2 | 451 | 0.0 | 773 |
| MS.gene010105.t1 | AT4G24550 | 81.067 | 375 | 70 | 1 | 1 | 375 | 2 | 375 | 0.0 | 635 |
| MS.gene010105.t1 | AT4G24550 | 81.117 | 376 | 70 | 1 | 1 | 376 | 2 | 376 | 0.0 | 634 |
| MS.gene010105.t1 | AT1G60780 | 34.590 | 451 | 264 | 11 | 3 | 451 | 6 | 427 | 4.38e-81 | 257 |
| MS.gene010105.t1 | AT1G10730 | 34.879 | 453 | 260 | 10 | 3 | 451 | 6 | 427 | 9.68e-81 | 256 |
| MS.gene010105.t1 | AT5G46630 | 32.533 | 458 | 275 | 11 | 3 | 451 | 6 | 438 | 8.24e-76 | 244 |
| MS.gene010105.t1 | AT5G46630 | 31.713 | 432 | 262 | 10 | 3 | 425 | 6 | 413 | 1.00e-67 | 223 |
| MS.gene010105.t1 | AT1G56590 | 27.226 | 393 | 246 | 9 | 1 | 389 | 1 | 357 | 1.38e-36 | 139 |
Find 95 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAGAGCTAAGCGAATTTC+AGG | 0.231119 | 5.2:+56026906 | None:intergenic |
| GGTAATATAACGAAAGAATC+TGG | 0.298492 | 5.2:-56022559 | MS.gene010105:CDS |
| TTTCCATGAATCTGTTCATT+TGG | 0.309246 | 5.2:-56025269 | MS.gene010105:CDS |
| TTGCAAATAGGAAAGAAATC+TGG | 0.318770 | 5.2:-56021659 | MS.gene010105:CDS |
| ATGCGAAAAGGAGGCTTAAA+TGG | 0.326302 | 5.2:+56025080 | None:intergenic |
| CAGGTAAAATATTTGCAAAT+AGG | 0.329271 | 5.2:-56021671 | MS.gene010105:CDS |
| TACTTTCATGTGAAAGTTGT+TGG | 0.332550 | 5.2:-56028773 | MS.gene010105:CDS |
| ATGTTTCTAGAGCAGGTTAT+AGG | 0.342498 | 5.2:-56025333 | MS.gene010105:intron |
| GTGCGGTGAAACAGATTAAT+GGG | 0.349197 | 5.2:-56022735 | MS.gene010105:intron |
| AGTGCGGTGAAACAGATTAA+TGG | 0.360372 | 5.2:-56022736 | MS.gene010105:intron |
| TATTATTTGAACCAGGATTT+CGG | 0.363405 | 5.2:-56028155 | MS.gene010105:intron |
| GCACAATGTATCACAGCTTC+AGG | 0.370690 | 5.2:-56022506 | MS.gene010105:intron |
| TAATACCTGGTGGTATATTT+CGG | 0.378845 | 5.2:+56024575 | None:intergenic |
| CAAATGAAGAGCTATCTTAC+TGG | 0.380878 | 5.2:-56026933 | MS.gene010105:CDS |
| TATCCAAATGAACAGATTCA+TGG | 0.383028 | 5.2:+56025266 | None:intergenic |
| TCTGTTGTTGCAACAGAGCC+TGG | 0.386144 | 5.2:-56027886 | MS.gene010105:CDS |
| AAGATAGCTCTTCATTTGTA+TGG | 0.387761 | 5.2:+56026938 | None:intergenic |
| ATATTGACTTCTGAGATTGA+TGG | 0.388047 | 5.2:-56026963 | MS.gene010105:CDS |
| ATAGTAAATGTCATGCTAAC+AGG | 0.392558 | 5.2:+56022535 | None:intergenic |
| ACATATCTAACCCACCGATA+TGG | 0.403897 | 5.2:+56021626 | None:intergenic |
| AAGGAGGGAGGAAATCTTTG+TGG | 0.417677 | 5.2:-56027857 | MS.gene010105:CDS |
| ACCATGTGATTCCTGAGAAA+AGG | 0.428446 | 5.2:+56022681 | None:intergenic |
| GACTTTGTAACAGCTATTCC+TGG | 0.429404 | 5.2:+56027907 | None:intergenic |
| AAAAGTAGATATCACCTTAA+GGG | 0.429427 | 5.2:+56025031 | None:intergenic |
| GAACTGGAGCCTGGTGCTAC+TGG | 0.430005 | 5.2:-56022890 | MS.gene010105:CDS |
| CTGGGTCGCATGAGCCATAT+CGG | 0.434703 | 5.2:-56021640 | MS.gene010105:CDS |
| AAGTGGCTCATTGAAAATAT+AGG | 0.434746 | 5.2:+56028097 | None:intergenic |
| TACTGTTTGTTGCAACTACA+AGG | 0.435206 | 5.2:-56028748 | MS.gene010105:CDS |
| GCAGCACGTCTGTCACCTCT+TGG | 0.441008 | 5.2:-56028065 | MS.gene010105:CDS |
| GTTTGTTGACAGAATGTAGA+TGG | 0.444498 | 5.2:-56028803 | MS.gene010105:intron |
| CTTCCCAAAACTTTACTTTG+CGG | 0.450669 | 5.2:+56029464 | None:intergenic |
| GTGTTATTAAAGATTACCTT+GGG | 0.455894 | 5.2:-56028676 | MS.gene010105:CDS |
| CGAAGAGGCAGGTTCCCTTA+AGG | 0.468484 | 5.2:-56025045 | MS.gene010105:intron |
| TGGTCCCTCGCTTCTTCCAA+TGG | 0.473383 | 5.2:+56026872 | None:intergenic |
| GAAAAGGAGGCTTAAATGGC+TGG | 0.473779 | 5.2:+56025084 | None:intergenic |
| AATATCTCTGCACTTCCCCT+TGG | 0.476578 | 5.2:+56029490 | None:intergenic |
| ATATCTCTGCACTTCCCCTT+GGG | 0.479873 | 5.2:+56029491 | None:intergenic |
| GCTTAAATGGCTGGGTCATA+CGG | 0.487133 | 5.2:+56025093 | None:intergenic |
| CTGTTGTTGCAACAGAGCCT+GGG | 0.489260 | 5.2:-56027885 | MS.gene010105:CDS |
| GCTAGAATGGAGTTTAAAGA+AGG | 0.489453 | 5.2:-56022834 | MS.gene010105:intron |
| AGGCAAATAAGAAGCTAGAA+TGG | 0.493385 | 5.2:-56022847 | MS.gene010105:CDS |
| TTGGGAAGATGGCGAACTGC+AGG | 0.495134 | 5.2:-56029449 | MS.gene010105:CDS |
| GCAGGTTATAGGAGTTCCTC+TGG | 0.496138 | 5.2:-56025322 | MS.gene010105:intron |
| GACGAAGAACTGTGAGATCA+TGG | 0.497880 | 5.2:+56030562 | None:intergenic |
| ATGACTGGAAATTCACCGTC+TGG | 0.498642 | 5.2:+56025122 | None:intergenic |
| ATCAAAGCATTGATGCGAAA+AGG | 0.498854 | 5.2:+56025068 | None:intergenic |
| CGTGTTATTAAAGATTACCT+TGG | 0.501304 | 5.2:-56028677 | MS.gene010105:CDS |
| TGCAAATAGGAAAGAAATCT+GGG | 0.503739 | 5.2:-56021658 | MS.gene010105:CDS |
| AGAAGCGAGGGACCAATCTC+CGG | 0.516323 | 5.2:-56026864 | MS.gene010105:intron |
| CTTAACGACGATCTTTCCAT+TGG | 0.518614 | 5.2:-56026888 | MS.gene010105:CDS |
| CAGATCGTGGTGAGGTCCCA+AGG | 0.521788 | 5.2:-56029507 | MS.gene010105:CDS |
| TATAGGAGTTCCTCTGGCTC+AGG | 0.522359 | 5.2:-56025316 | MS.gene010105:CDS |
| AAGATAATACCTACAAAGAC+AGG | 0.540202 | 5.2:+56029418 | None:intergenic |
| TTCCTCTGGCTCAGGAGCAG+TGG | 0.541824 | 5.2:-56025308 | MS.gene010105:CDS |
| AGGCAAATTCCTACGTTGCT+CGG | 0.542245 | 5.2:-56021598 | MS.gene010105:CDS |
| CTGGTGGTATATTTCGGTAG+TGG | 0.545193 | 5.2:+56024581 | None:intergenic |
| GTAATATAACGAAAGAATCT+GGG | 0.547603 | 5.2:-56022558 | MS.gene010105:CDS |
| GTCATACGGTAATTCATGAC+TGG | 0.553624 | 5.2:+56025107 | None:intergenic |
| CTTACAGAAAAGATTGCAGC+AGG | 0.554533 | 5.2:+56028041 | None:intergenic |
| TGTTATCGCCACGCTGTGAA+AGG | 0.565981 | 5.2:+56030540 | None:intergenic |
| GAGAGAGAAGCTAACAGTCA+CGG | 0.578733 | 5.2:+56030510 | None:intergenic |
| CTTTCCATTGGAAGAAGCGA+GGG | 0.579543 | 5.2:-56026876 | MS.gene010105:CDS |
| CATCAATGCTTTGATCGAAG+AGG | 0.580364 | 5.2:-56025060 | MS.gene010105:CDS |
| AATGCTTTGATCGAAGAGGC+AGG | 0.581261 | 5.2:-56025056 | MS.gene010105:CDS |
| AACAAACAGAACTCTATCCC+TGG | 0.584190 | 5.2:-56025236 | MS.gene010105:intron |
| AAAAGCTCGAAGACGAAGGA+AGG | 0.594557 | 5.2:+56028713 | None:intergenic |
| CACTACCGAAATATACCACC+AGG | 0.599182 | 5.2:-56024580 | MS.gene010105:intron |
| CTTTATTGCAGATCGTGGTG+AGG | 0.600117 | 5.2:-56029515 | MS.gene010105:intron |
| AAAAGGAGGCTTAAATGGCT+GGG | 0.600629 | 5.2:+56025085 | None:intergenic |
| GGAAGATGGCGAACTGCAGG+AGG | 0.601110 | 5.2:-56029446 | MS.gene010105:CDS |
| TGTTGTTGCAACAGAGCCTG+GGG | 0.604072 | 5.2:-56027884 | MS.gene010105:CDS |
| TACCACTGCTCCTGAGCCAG+AGG | 0.608309 | 5.2:+56025306 | None:intergenic |
| AGTGTTTGCTGTTATACTTG+AGG | 0.609838 | 5.2:+56024619 | None:intergenic |
| GTCGCATGAGCCATATCGGT+GGG | 0.614018 | 5.2:-56021636 | MS.gene010105:CDS |
| TCTTTCCATTGGAAGAAGCG+AGG | 0.615882 | 5.2:-56026877 | MS.gene010105:CDS |
| AGATCGTGGTGAGGTCCCAA+GGG | 0.619776 | 5.2:-56029506 | MS.gene010105:CDS |
| TTGTAAAAGCTCGAAGACGA+AGG | 0.620652 | 5.2:+56028709 | None:intergenic |
| GTTGTCTGTCCAGTAGCACC+AGG | 0.621982 | 5.2:+56022881 | None:intergenic |
| AGCTGTGATACATTGTGCAT+TGG | 0.624337 | 5.2:+56022511 | None:intergenic |
| AAAGCATTGATGCGAAAAGG+AGG | 0.626027 | 5.2:+56025071 | None:intergenic |
| CGTGCTGCATCAATTACAAG+TGG | 0.630367 | 5.2:+56028080 | None:intergenic |
| AAGATTGCAGCAGGTCCAAG+AGG | 0.631025 | 5.2:+56028050 | None:intergenic |
| GGTCGCATGAGCCATATCGG+TGG | 0.633753 | 5.2:-56021637 | MS.gene010105:CDS |
| ACTGTTTGTTGCAACTACAA+GGG | 0.643129 | 5.2:-56028747 | MS.gene010105:CDS |
| TTCTTCGTCCTTTCACAGCG+TGG | 0.643685 | 5.2:-56030548 | MS.gene010105:CDS |
| CATCGAGTAACTCATACACC+AGG | 0.653609 | 5.2:+56028618 | None:intergenic |
| TGCGGTGAAACAGATTAATG+GGG | 0.660205 | 5.2:-56022734 | MS.gene010105:intron |
| AGTCTTCATTGAGAATCCCA+AGG | 0.662867 | 5.2:+56028660 | None:intergenic |
| ACTGGAAATTCACCGTCTGG+AGG | 0.672021 | 5.2:+56025125 | None:intergenic |
| GTGGGTTAGATATGTTACAC+AGG | 0.675410 | 5.2:-56021618 | MS.gene010105:CDS |
| GCATCATATCCGAGCAACGT+AGG | 0.688345 | 5.2:+56021589 | None:intergenic |
| ATAATACCTACAAAGACAGG+TGG | 0.692932 | 5.2:+56029421 | None:intergenic |
| GTTGTTGCAACAGAGCCTGG+GGG | 0.698165 | 5.2:-56027883 | MS.gene010105:CDS |
| GCGGTGAAACAGATTAATGG+GGG | 0.708079 | 5.2:-56022733 | MS.gene010105:intron |
| GATCGTGGTGAGGTCCCAAG+GGG | 0.723226 | 5.2:-56029505 | MS.gene010105:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 56021592 | 56030582 | 56021592 | ID=MS.gene010105 |
| chr5.2 | mRNA | 56021592 | 56030582 | 56021592 | ID=MS.gene010105.t1;Parent=MS.gene010105 |
| chr5.2 | exon | 56030525 | 56030582 | 56030525 | ID=MS.gene010105.t1.exon1;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56030525 | 56030582 | 56030525 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56029432 | 56029526 | 56029432 | ID=MS.gene010105.t1.exon2;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56029432 | 56029526 | 56029432 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56028610 | 56028813 | 56028610 | ID=MS.gene010105.t1.exon3;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56028610 | 56028813 | 56028610 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56028046 | 56028162 | 56028046 | ID=MS.gene010105.t1.exon4;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56028046 | 56028162 | 56028046 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56027819 | 56027947 | 56027819 | ID=MS.gene010105.t1.exon5;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56027819 | 56027947 | 56027819 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56026865 | 56026991 | 56026865 | ID=MS.gene010105.t1.exon6;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56026865 | 56026991 | 56026865 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56025237 | 56025340 | 56025237 | ID=MS.gene010105.t1.exon7;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56025237 | 56025340 | 56025237 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56025046 | 56025150 | 56025046 | ID=MS.gene010105.t1.exon8;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56025046 | 56025150 | 56025046 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56024581 | 56024675 | 56024581 | ID=MS.gene010105.t1.exon9;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56024581 | 56024675 | 56024581 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56022835 | 56022922 | 56022835 | ID=MS.gene010105.t1.exon10;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56022835 | 56022922 | 56022835 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56022683 | 56022743 | 56022683 | ID=MS.gene010105.t1.exon11;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56022683 | 56022743 | 56022683 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56022507 | 56022580 | 56022507 | ID=MS.gene010105.t1.exon12;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56022507 | 56022580 | 56022507 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| chr5.2 | exon | 56021592 | 56021690 | 56021592 | ID=MS.gene010105.t1.exon13;Parent=MS.gene010105.t1 |
| chr5.2 | CDS | 56021592 | 56021690 | 56021592 | ID=cds.MS.gene010105.t1;Parent=MS.gene010105.t1 |
| Gene Sequence |
| Protein sequence |
